Updated on 2024/10/08

写真a

 
ICHINOSE HIROFUMI
 
Organization
Faculty of Agriculture Department of Agro-environmental Sciences Associate Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Agro-environmental Sciences(Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Profile
【研究活動】 担子菌の物質代謝能を支える細胞応答機構の解明とその利用を目指し、 担子菌シトクロームP450の機能解析と応用に関する研究を行っている。また担子菌をバイオプロセスへ向けた生物資源と考え、有用酵素・有用代謝産物の発掘・創出を目指している。 【教育・社会活動】研究活動を通じて学生各人の考察力・発想力・想像力を伸ばし、多様な社会で活躍できる人材の育成を目指している。特に、大学院生には知識・技術の習得にとどまらず、新しい課題を自ら発掘して課題解決策を見いだす力を養ってもらいたいと考えている。研究成果を通じた社会貢献への第一歩として、論文発表や学会発表による外部公表には積極的に取り組むよう指導している。
External link

Degree

  • PhD

Research Interests・Research Keywords

  • Research theme: Biotechnology of Basidiomycetes

    Keyword: Basidiomycetes, Cytochrome P450

    Research period: 1996.4

Awards

  • 日本生物工学会 第14回論文賞

    2006.9   日本生物工学会  

  • 日本生物工学会 第12回論文賞

    2004.9   日本生物工学会  

  • 日本化学会 第76春季年会ポスター賞

    1999.5   日本化学会  

Papers

  • Conversion and synthesis of chemicals catalyzed by fungal cytochrome P450 monooxygenases: A review. International journal

    Dani Permana, Takuya Kitaoka, Hirofumi Ichinose

    Biotechnology and bioengineering   120 ( 7 )   1725 - 1745   2023.7   ISSN:0006-3592 eISSN:1097-0290

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biotechnology and Bioengineering  

    Cytochrome P450s (also called CYPs or P450s) are a superfamily of heme-containing monooxygenases. They are distributed in all biological kingdoms. Most fungi have at least two P450-encoding genes, CYP51 and CYP61, which are housekeeping genes that play important roles in the synthesis of sterols. However, the kingdom fungi is an interesting source of numerous P450s. Here, we review reports on fungal P450s and their applications in the bioconversion and biosynthesis of chemicals. We highlight their history, availability, and versatility. We describe their involvement in hydroxylation, dealkylation, oxygenation, C═C epoxidation, C-C cleavage, C-C ring formation and expansion, C-C ring contraction, and uncommon reactions in bioconversion and/or biosynthesis pathways. The ability of P450s to catalyze these reactions makes them promising enzymes for many applications. Thus, we also discuss future prospects in this field. We hope that this review will stimulate further study and exploitation of fungal P450s for specific reactions and applications.

    DOI: 10.1002/bit.28411

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  • Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon Reviewed International journal

    Natsuki Masunaga, Takuya Kitaoka, Hirofumi Ichinose

    Microbial Biotechnology   16 ( 3 )   632 - 644   2023.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/1751-7915.14204

  • Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene Reviewed International journal

    Hirofumi Ichinose, Shota Ukeba, Takuya Kitaoka

    Enzyme Microb Technol   158   110037   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1016/j.enzmictec.2022.110037

  • Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene. Reviewed International journal

    Hirofumi Ichinose, Shota Ukeba, Takuya Kitaoka

    Enzyme and microbial technology   158   110037 - 110037   2022.8   ISSN:0141-0229 eISSN:1879-0909

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Enzyme and Microbial Technology  

    Basidiomycetes produce various sesquiterpenoids and their relevance for pharmaceutical and agricultural applications and understanding their biosynthetic machinery to produce these secondary metabolites have attracted significant interest. Because sesquiterpene synthases (STSs) and cytochrome P450 monooxygenases (P450s) play pivotal roles in the production of sesquiterpenoids, functional characterization of these enzymes is fundamentally essential. In this study, we found 11 possible STSs from the white-rot basidiomycete Phanerochaete chrysosporium (PcSTSs) and isolated nine of these as full-length cDNAs encoding a mature open reading frame. Using the isolated cDNAs, we performed heterologous expression of PcSTSs in Saccharomyces cerevisiae. Metabolic studies revealed that seven of the PcSTSs produce a series of sesquiterpene scaffolds, including (E)-α-bisabolene. Furthermore, we constructed a co-expression system of (E)-α-bisabolene synthase and P450 from P. chrysosporium (PcCYP). Semi-comprehensive screening using 120 isoforms of PcCYPs resulted in the identification of CYP5158A1 and CYP5144C8, two P450s capable of decorating (E)-α-bisabolene.

    DOI: 10.1016/j.enzmictec.2022.110037

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  • Latent Functions and Applications of Cytochrome P450 Monooxygenases from Thamnidium elegans: A Novel Biocatalyst for 14α-Hydroxylation of Testosterone. Reviewed International journal

    Dani Permana, Ksenia Niesel, Mark James Ford, Hirofumi Ichinose

    ACS omega   7 ( 16 )   13932 - 13941   2022.4   ISSN:2470-1343

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACS Omega  

    Cytochrome P450 monooxygenases (P450s) are ubiquitous enzymes with high availability and diversity in nature. Fungi provide a diverse and complex array of P450s, and these enzymes play essential roles in various secondary metabolic processes. Besides the physiological impacts of P450s on fungal life, their versatile functions are attractive for use in advanced applications of the biotechnology sector. Herein, we report gene identification and functional characterization of P450s from the zygomycetous fungus Thamnidium elegans (TeCYPs). We identified 48 TeCYP genes, including two putative pseudogenes, from the whole-genome sequence of T. elegans. Furthermore, we constructed a functional library of TeCYPs and heterologously expressed 46 TeCYPs in Saccharomyces cerevisiae. Recombinants of S. cerevisiae were then used as whole-cell biocatalysts for bioconversion of various compounds. Catalytic potentials of various TeCYPs were demonstrated through a functionomic survey to convert a series of compounds, including steroidal substrates. Notably, CYP5312A4 was found to be highly active against testosterone. Based on nuclear magnetic resonance analysis, enzymatic conversion of testosterone to 14α-hydroxytestosterone by CYP5312A4 was demonstrated. This is the first report to identify a novel fungal P450 that catalyzes the 14α-hydroxylation of testosterone. In addition, we explored the latent potentials of TeCYPs using various substrates. This study provides a platform to further study the potential use of TeCYPs as catalysts in pharmaceutical and agricultural industries and biotechnology.

    DOI: 10.1021/acsomega.2c00430

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Presentations

  • Functional library of fungal cytochrome P450 Invited International conference

    Yuuki Sanada, Hirofumi Ichinose, Hiroyuki Wariishi

    50th Anniversary Symposium on Cytochrome P450 in Fukuoka  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • Functional screening of cytochrome P450s for production of bioactive flavonoids Invited International conference

    Mayumi Hatakeyama, Hirofumi Ichinose, Hiroyuki Wariishi

    50th Anniversary Symposium on Cytochrome P450 in Fukuoka  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • Molecular and functional diversity of fungal cytochrome P450 Invited International conference

    Hirofumi Ichinose

    50th Anniversary Symposium on Cytochrome P450 in Fukuoka  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  • 真核微生物シトクロム P450 の機能ライブラリー

    眞田有規, 一瀬 博文, 割石 博之

    日本生物工学会 64回大会  2012.10 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  • 生理活性フラボノイドの産生へ向けたシトクロム P450 機能の探索

    畠山真由美, 一瀬 博文, 割石 博之

    日本生物工学会 64回大会  2012.10 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

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MISC

  • Cytochrome P450 of wood-rotting basidiomycetes and biotechnological applications

    Hirofumi Ichinose

    Biotechnology and Applied Biochemistry. 2013;60:71-81.   2013.1

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Molecular and functional diversity of fungal cytochrome P450s

    Ichinose Hirofumi

    Biol Pharm Bull. 2012;35(6):833-7.   2012.6

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • キノコがダイオキシンを壊すわけ

    割石博之, 一瀬博文

    生物工学会誌   2003.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • キノコの仲間が環境を浄化する -担子菌における芳香族及びヘテロ環化合物分解能の多様性・多機能性-

    割石博之, 一瀬博文

    化学と生物   2000.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

Industrial property rights

Patent   Number of applications: 7   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 日本化学会

  • 日本生物工学会

  • 日本木材学会

  • リグニン学会

Academic Activities

  • 組織委員、座長

    2023 International Joint Meeting of the 23rd International Conference on Cytochrome P450 and the 38th Annual Meeting of the Japanese Society for the Study of Xenobiotics  ( Japan ) 2023.9

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    Type:Competition, symposium, etc. 

  • 実行委員

    第73回日本木材学会大会  ( Japan ) 2023.3

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    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2021

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

  • 実行委員(総務)

    第67回日本木材学会大会  ( Japan ) 2017.3

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    Type:Competition, symposium, etc. 

  • 実行委員 International contribution

    50th Anniversary Symposium on Cytochrome P450 in Fukuoka  ( Japan ) 2013.12

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    Type:Competition, symposium, etc. 

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Research Projects

  • Development of Nanostructured Scaffolds for Stem Cell Culture to Directly Regulate Cellular Functions via Natural Polysaccharide Nanofibers

    Grant number:23H00345  2023.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    北岡 卓也, 一瀬 博文

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    Grant type:Scientific research funding

    林産多糖資源のセルロースや海産多糖のキチンに代表される天然多糖類は、人工合成が不可能な伸びきり鎖結晶のナノ構造体であり、ナノファイバー形状と二糖単位の規則的構造を特徴とする。一方、ヒトの体細胞を取り囲む細胞外マトリックス(ECM)も、線維形状のコラーゲンや、ヒアルロン酸などの二糖繰り返し多糖で構成されている。本研究では、この意外な構造類似性に着目し、(1)多糖ナノファイバーの形状・物性制御、(2)生体官能基の界面導入とECMの構造模倣、(3)多糖ナノ界面が幹細胞に直接働きかける作用機序の探究により、天然構造多糖が幹細胞培養の鍵を握る「ゼノフリー幹細胞制御培養基材」を創発する。

    CiNii Research

  • Search of the enzyme and the gene for terpene synthesis in Cryptomeria japonica

    Grant number:23K05339  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    藤田 弘毅, 一瀬 博文

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    Grant type:Scientific research funding

    スギは日本を代表する樹種で生産量/材積も特に大きいことから建材目的で物理的性質や
    対腐朽性は詳細に調べられている。一方、近年、匂い(心材テルペン)の居住者に対する良好な心理・生理効果も報告されていることから、スギの香り成分は付加価値創造に重要である。ところが、スギの遺伝子情報は公開されているが、匂い関連成分に関するデータベース解析は行われていない。
    そこで、主に既存データベースからデータを抽出し、スギの代表的抽出成分の生合成関連遺伝子/酵素を明らかにすることで、品種・環境で特徴的とされるテルペン組成の違いを生化学的観点から解明することを目標とする。

    CiNii Research

  • Research for anti-termite chemicals using terpenoids produced by fungal cytochrome P450monooxygenase recombinant yeast.

    Grant number:22K05778  2022.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    須原 弘登, 一瀬 博文, 加藤 政和

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    Grant type:Scientific research funding

    一般的に『精油』として知られるテルペン類の中には、α-cadinolやsandaracopimarinol、16-phyllocladanolのように殺蟻活性や抗蟻(摂食抑制)活性を示すものがあることが知られている。一方で、精油は生産量が僅かである場合が多く、多種のテルペンの混合物として得られるため、テルペンの機能性を生かした防蟻処理についてはあまり研究が進んでいない。
    <BR>
    本研究では糸状菌のSTS及び様々な化合物に対して代謝能を持つ糸状菌のCYP遺伝子を酵母に導入しテルペン類の生産・代謝を行い、これにより生産されたテルペンを用いて防蟻性能試験を行うことで、天然物であるテルペンまたはその生物変換物による防蟻処理技術の開発を目指す

    CiNii Research

  • 担子菌に封印されたセスキテルペン合成酵素:逆進化でひもとく真機能

    Grant number:22K19212  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 白色腐朽菌の環境汚染物質代謝能の意義解明及び汚染環境浄化への発展的応用

    Grant number:21H04744  2021.4 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    平井 浩文, 一瀬 博文, 鈴木 智大, 長井 薫, 森 智夫

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    Grant type:Scientific research funding

    本研究課題では、環境汚染物質代謝シトクロムP450(CYP)ライブラリーの構築、及び白色腐朽菌の与える分解代謝産物の生物学的意義の解明を遂行し、これらの知見を総合して目的CYP遺伝子を高発現させた白色腐朽菌Phanerochaete sordida YK-624株による汚染環境修復の実証試験を実施し、汎用性が極めて高い画期的浄化技術を開発し、SDGsにおける達成目標に貢献する地球環境グリーンイノベーションを推進する。

    CiNii Research

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Educational Activities

  • I am in charge of lectures on forest science and biotechnology. I strive to increase basic knowledge of student in these areas. I give specialized knowledge for students by providing opportunities to have discussion on cutting-edge research topics in seminars. In addition, I aim to improve presentation skills of students through practice.

Class subject

  • 木質微生物学

    2024.12 - 2025.2   Winter quarter

  • 製紙プロセス工学

    2024.10 - 2025.3   Second semester

  • 基幹教育セミナー

    2024.6 - 2024.8   Summer quarter

  • 森林生物工学

    2024.6 - 2024.8   Summer quarter

  • Biomaterial Science Ⅰ

    2024.6 - 2024.8   Summer quarter

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FD Participation

  • 2022.3   Role:Participation   Title:リポジトリ登録を対象とした大学改革推進経費の新指標案について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.3   Role:Participation   Title:農学研究院FD:リポジトリ登録を対象とした大学改革推進経費の新指標案について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.11   Role:Participation   Title:人を対象とする生命科学・医学系研究に関する倫理指針

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.11   Role:Participation   Title:農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role:Participation   Title:大学の研究評価の現状と農学研究院の「部局独自の評価基準」案における業績分析

    Organizer:[Undergraduate school/graduate school/graduate faculty]

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