Updated on 2024/10/12

Information

 

写真a

 
TAKEGAWA KAORU
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Professor
Contact information
メールアドレス
Tel
0928024732
Profile
Vesicle-mediated protein trafficking in yeasts. Heterologous protein production in yeast. Structure and physiological role for fungal glycoproteins.
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Research Areas

  • Life Science / Applied microbiology

Degree

  • Ph.D

Research History

  • Kyushu University Faculty of Agriculture Professor

    2008.4 - Present

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  • 昭和62年ー平成6年 香川大学農学部 助手 平成6年ー平成14年 香川大学農学部 助教授 平成14年ー平成20年 香川大学農学部 教授

Education

  • 京都大学大学院   農学研究科   食品工学専攻

    - 1986

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    Country: Japan

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Research Interests・Research Keywords

  • Research theme:Applied Microbiology

    Keyword:Applied Microbiology

    Research period: 2024

  • Research theme:応用微生物学

    Keyword:応用微生物学

    Research period: 2024

  • Research theme:Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system.

    Keyword:Fission yeast, heterologous proteins, gene expression

    Research period: 1997.4

  • Research theme:Vesicle-mediated protein transport pathways to the vacuole in yeast

    Keyword:Yeast, vacuole, vesicle trafficking, protein degradation

    Research period: 1991.10

  • Research theme:Transglycosylation activity and its application of endo-β-N-acetylglucosaminidase

    Keyword:Endoglycosidase, transglycosylation activity, neoglycoconjugates

    Research period: 1991.4

  • Research theme:Structures and physiological roles of protein glycosylation in microbial eukaryotes.

    Keyword:Glycoprotein, sugar chains

    Research period: 1984.4

Awards

  • 日本農芸化学会農芸化学奨励賞

    2000.4   日本農芸化学会   糖タンパク質糖鎖の機能解析とそのリモデリングに関する基礎および応用研究

Papers

  • Structural basis for specific cleavage of core-fucosylated N-glycans by endo-beta-N-acetylglu-cosaminidase from Cordyceps militaris. Reviewed International journal

    Seki H, #Huang Y, Arakawa T, Yamada C, Kinoshita T, Iwamoto, Higuchi Y, Takegawa K, Fushinobu S

    Journal of Biological Chemistry   2019.10

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    DOI: 10.1074/jbc.RA119.010842.

  • Identification and characterization of a novel β-D-galactosidase that releases pyruvylated ga-lactose. Reviewed International journal

    Higuchi Y., Matsufuji H., Tanuma M., Arakawa T., Mori K., Yamada C., Shofia R., Matsunaga E., Tashiro K., Fushinobu S., Takegawa K.

    Scientific Reports   8 ( 1 )   2018.9

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    Pyruvyl modification of oligosaccharides is widely seen in both prokaryotes and eukaryotes. Although the biosynthetic mechanisms of pyruvylation have been investigated, enzymes that metabolize and degrade pyruvylated oligosaccharides are not well known. Here, we searched for a pyruvylated galactose (PvGal)-releasing enzyme by screening soil samples. We identified a Bacillus strain, as confirmed by the 16S ribosomal RNA gene analysis, that exhibited PvGal-ase activity toward p-nitrophenyl-β-D-pyruvylated galactopyranose (pNP-β-D-PvGal). Draft genome sequencing of this strain, named HMA207, identified three candidate genes encoding potential PvGal-ases, among which only the recombinant protein encoded by ORF1119 exhibited PvGal-ase activity. Although ORF1119 protein displayed broad substrate specificity for pNP sugars, pNP-β-D-PvGal was the most favorable substrate. The optimum pH for the ORF1119 PvGal-ase was determined as 7.5. A BLAST search suggested that ORF1119 homologs exist widely in bacteria. Among two homologs tested, BglC from Clostridium but not BglH from Bacillus showed PvGal-ase activity. Crystal structural analysis together with point mutation analysis revealed crucial amino acids for PvGal-ase activity. Moreover, ORF1119 protein catalyzed the hydrolysis of PvGal from galactomannan of Schizosaccharomyces pombe, suggesting that natural polysaccharides might be substrates of the PvGal-ase. This novel PvGal-catalyzing enzyme might be useful for glycoengineering projects to produce new oligosaccharide structures.

    DOI: 10.1038/s41598-018-30508-4

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  • Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oli-gosaccharides and human IgG. Reviewed International journal

    2018.1

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  • A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide. Reviewed International journal

    Higuchi Y, Yoshinaga S, Yoritsune K, Tateno H, Hirabayashi J, Nakakita S, Kanekiyo M, Kakuta Y, Takegawa K

    Scientific Reports   2016.5

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  • Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea Reviewed International journal

    2015.7

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  • gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus. Reviewed International journal

    Komachi Y, Hatakeyama S, Motomatsu H, Futagami T, Kinzjakina K, Sorbado P, Ekino K, Takegawa K, Goto M, Nomura Y, Oka T

    Molecular Microbiology   2013.12

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  • The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters. Reviewed International journal

    2013.9

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    Rheb GTPaseであるTsc1-Tsc2複合体は細胞外の環境に応答して細胞成長調節において重要な役割を果たしている。分裂酵母においてTsc1-Tsc2複合体は細胞周期の進行やアミノ酸の取り込みなどを調節することが報告されていた。我々はE3ユビキチンリガーゼであるPub1とβ-アレスチン様タンパクであるAny1が細胞膜アミノ酸トランスポーターであるAat1の局在を制御していることを見出した。またPub1とAny1が分裂酵母細胞内で相互作用していることからTSC-Rhebシグナル経路がPub1やAny1を通じて細胞膜のアミノ酸トランスポーターの局在を制御していることを明らかにした。

  • Characterization of genome-reduced fission yeast strains. Reviewed International journal

    Sasaki M, Kumagai H, Takegawa K, Tohda H

    Nucleic Acid Research   2013.5

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  • Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe. Reviewed International journal

    2012.11

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    分裂酵母の糖鎖中には多量のガラクトースが含まれている。糖鎖構造解析の結果、ガラクトース残基はα1,2-およびα1,3-結合で付加している。分裂酵母ゲノム中に存在する全てのα1,2-ガラクトース転移酵素遺伝子を破壊した株を作成したところ、まだα1,3-結合のガラクトースが残っていることを見出した。そこでさらにゲノムを解析したところ、仮想糖転移酵素が3遺伝子存在することがわかり、これらを破壊した10重破壊株を作成したところ、糖鎖のガラクトース残基は完全に欠失することを明らかにした。また大腸菌で生産させたこれらの遺伝子産物が実際にα1,3-ガラクトース転移活性を示すこともわかった。以上の結果から分裂酵母に存在する新規α1,3-ガラクトース転移酵素の諸性質を明らかにすることができた。

  • Identification of galactose-specific flocculin essential for nonsexual flocculation and hyphal growth in Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Morita T, Tanaka N, Tohda H, Takegawa K

    Molecular Microbiology   2011.12

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  • Loss-of- and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis. Reviewed International journal

    Hirano Y, Matsuzawa T, Takegawa K, Sato MH

    Plant Physiology   2011.2

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  • Mannosylinositol phosphorylceramide is a main phospholipid component and required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe. Reviewed International journal

    Nakase M, Tani M, Morita T, Kitamoto HK, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, Takegawa K

    Journal of Cell Science   2010.5

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  • Role of septins in the orientation of forespore-membrane extension during sporulation in fission yeast. Reviewed International journal

    Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bahler J, Wu J-Q, Takegawa K, Tachikawa H, Pringle JR, Fukui Y

    Molecular and Cellular Biology   2010.4

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  • Critical roles of Mucin 1 glycosylation by transactivated polypeptide N-acetylgalactosaminyltransferase 6 in mammary carcinogenesis. Reviewed International journal

    Park J-H, Nishidate T, Kijima K, Ohashi T, Takegawa K, Hirata K, Nakamura Y, Katagiri T

    Cancer Research   2010.4

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  • Two Schizosaccharomyces pombe Rab7 homologs, Ypt7 and Ypt71, play antagonistic roles in the regulation of vacuolar morphology. Reviewed International journal

    Kashiwazaki J, Iwaki T, Takegawa K, Shimoda C, Nakamura T

    Traffic   2009.7

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  • A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast. Reviewed International journal

    Hirashima K, Iwaki T, Takegawa K, Giga-Hama Y, Tohda H

    Nucleic Acids Res.   2006.2

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  • Live cell imaging of β-tubulin mRNA reveals spatiotemporal expression dynamics in the filamentous fungus Aspergillus oryzae

    Keishu Kawatomi, Yuki Morita, Yoshinori Katakura, Kaoru Takegawa, Adokiye Berepiki, Yujiro Higuchi

    Scientific Reports   14 ( 1 )   13797   2024.6   ISSN:2045-2322 eISSN:2045-2322

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    In filamentous fungi, microtubules are important for polar growth and morphological maintenance and serve as rails for intracellular trafficking. The molecular mechanisms associated with microtubules have been analyzed. However, little is known about when and where tubulin, a component of microtubules, is biosynthesized in multinuclear and multicellular filamentous fungi. In this study, we visualized microtubules based on the enhanced green fluorescence protein (EGFP)-labeled α-tubulin and β-tubulin mRNA tagged by the EGFP-mediated MS2 system in living yellow Koji mold Aspergillus oryzae cells in order to understand the spatiotemporal production mechanism of tubulin. We found that mRNA of btuA, encoding for β-tubulin, localized at dot-like structures through the apical, middle and basal regions of the hyphal cells. In addition, some btuA mRNA dots showed microtubule-dependent motor protein-like dynamics in the cells. Furthermore, it was found that btuA mRNA dots were decreased in the cytoplasm just before mitosis but increased immediately after mitosis, followed by a gradual decrease. In summary, the localization and abundance of β-tubulin mRNA is spatiotemporally regulated in living A. oryzae hyphal cells.

    DOI: 10.1038/s41598-024-64531-5

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    Other Link: https://www.nature.com/articles/s41598-024-64531-5

  • Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells

    Yuki Morita, Kaoru Takegawa, Brett M. Collins, Yujiro Higuchi

    Microbiological Research   282   127653 - 127653   2024.5   ISSN:0944-5013 eISSN:1618-0623

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    DOI: 10.1016/j.micres.2024.127653

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  • Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells. Reviewed International journal

    Morita Y, Takegawa K, Collins BM, Higuchi Y

    Microbiological Research   2024.3

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  • Characterization of novel endo-β-N-acetylglucosaminidases from intestinal Barnesiella intestinihominis that hydrolyze multi-branched complex-type N-glycans. Reviewed

    2024.2

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  • Characterization of novel endo-β-N-acetylglucosaminidases from intestinal Barnesiella intestinihominis that hydrolyze multi-branched complex-type N-glycans

    Kanako Doi, Ai Mitani, Shin-ichi Nakakita, Yujiro Higuchi, Kaoru Takegawa

    Journal of Bioscience and Bioengineering   137 ( 2 )   101 - 107   2024.2   ISSN:1389-1723 eISSN:1347-4421

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    DOI: 10.1016/j.jbiosc.2023.12.004

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  • Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated beta1,3-galactose of N-linked oligosaccharides. Reviewed

    Fukunaga T, Watanabe M, Nakamichi Y, Morita T, Higuchi Y, Maekawa H, Takegawa K

    Journal of Bioscience and Bioengineering   2023.8

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    DOI: 10.1016/j.jbiosc.2023.03.002.

  • Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated β1,3-galactose of N-linked oligosaccharides.

    Takamasa Fukunaga, Masahiro Watanabe, Yusuke Nakamichi, Tomotake Morita, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa

    Journal of bioscience and bioengineering   135 ( 6 )   423 - 432   2023.6   ISSN:1389-1723 eISSN:1347-4421

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    N-linked oligosaccharides in the fission yeast Schizosaccharomyces pombe contain large amounts of d-galactose (Gal), which mainly comprises α1,2- and α1,3-linked Gal except for pyruvylated β1,3-linked Gal (PvGalβ) at the non-reducing end. The PvGalβ unit of N-glycans is important for regulating nonsexual flocculation and invasive growth, but the mechanistic basis for β-galactosylation in fission yeast is poorly understood. To gain insight into this mechanism, we have characterized three genes previously identified to be involved in PvGalβ biosynthesis (pvg2, pvg3, and pvg5), with a focus on pvg3, which is predicted to contain a domain conserved in galactosyltransferase family 31 (GT31) proteins. Fluorescent microscopy revealed that Pvg3 is stably localized at the Golgi membrane, regardless of the presence of pvg2+ or pvg5+, suggesting that Pvg2 and Pvg5 are essential for the function of Pvg3 as a β1,3-galactosyltransferase, and not for its localization to the Golgi. Mutation of the GT31 family DXD motif and GT-A fold in Pvg3 resulted in loss of catalytic activity in vivo, supporting the idea that Pvg3 is a GT-A type β1,3-galactosyltransferase. Docking simulations further indicated that Pvg3 can recognize donor and acceptor substrates suitable for β-(1 → 3) bond formation. Yeast two-hybrid assay showed that Pvg5 physically interacts with Pvg3 and the pyruvyltransferase Pvg1. Collectively, these results provide insight into β-galactosylation catalyzed by Pvg3 and the supporting role of Pvg5 in PvGalβ biosynthesis.

    DOI: 10.1016/j.jbiosc.2023.03.002

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  • KH-17, a simplified derivative of bongkrekic acid, weakly inhibits the mitochondrial ADP/ATP carrier from both sides of the inner mitochondrial membrane

    Takegawa, K; Ito, T; Yamamoto, A; Yamazaki, N; Shindo, M; Shinohara, Y

    CHEMICAL BIOLOGY & DRUG DESIGN   101 ( 4 )   865 - 872   2023.4   ISSN:1747-0277 eISSN:1747-0285

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  • 真核微生物の細胞表層糖鎖マーカーとしての酸性糖鎖の選別機構と生理的役割の解明

    竹川 薫

    発酵研究所助成研究報告集   37 ( 0 )   134   2023   ISSN:00738751 eISSN:27592553

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    Language:Japanese   Publisher:公益財団法人 発酵研究所  

    DOI: 10.60396/iforc.37.0_134

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  • Galactosylation of cell-surface glycoprotein required for hyphal growth and cell wall integrity in Schizosaccharomyces japonicus. Reviewed

    #Fukunaga T, Ohashi T, Tanaka Y, Yoshimatsu T, Higuchi Y, Maekawa H, Takegawa K

    Journal of Bioscience and Bioengineering   2022.11

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    DOI: doi: 10.1016/j.jbiosc.2022.07.014.

  • Galactosylation of cell-surface glycoprotein required for hyphal growth and cell wall integrity in Schizosaccharomyces japonicus.

    Takamasa Fukunaga, Takao Ohashi, Yutaka Tanaka, Tomoki Yoshimatsu, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa

    Journal of bioscience and bioengineering   134 ( 5 )   384 - 392   2022.11   ISSN:1389-1723 eISSN:1347-4421

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    Schizosaccharomyces japonicus is a dimorphic yeast, transiting between unicellular and hyphal growth. The glycoproteins of fission yeast contain, in addition to mannose (Man), a large number of galactose (Gal) residues. Previously, we reported that the cell-surface O-glycans of S. japonicus comprise mainly tri-saccharides (Gal-Man-Man) as a main component, in contrast to the tetra-saccharides observed in other Schizosaccharomyces species. Here we have investigated the function of cell-surface Gal residues in S. japonicus. Because disruption of gms1+, encoding the UDP-Gal transporter required for galactomannan synthesis, abolishes cell-surface galactosylation in Schizosaccharomyces pombe, we constructed a deletion mutant of the homologous gene in S. japonicus gms1Δ [gms1 (S.j)] and determined the N- and O-linked oligosaccharide structures present on the cell surface. Disruption of gms1 (S.j) resulted in a complete lack of Gal on the cell surface, indicating that Gms1 plays an essential role in supplying UDP-Gal from the cytoplasm to the Golgi lumen. Analytical microscopy of gms1Δ demonstrated that the lack of cell-surface Gal did not affect cell growth or morphology during vegetative growth. However, hyphal development was blocked in gms1Δ, even in the presence of the topoisomerase I inhibitor camptothecin, which is known to induce hyphal differentiation in wild-type S. japonicus. Collectively, these findings show that Gal-containing oligosaccharides are required for cell wall integrity during filamentous growth in S. japonicus.

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  • In vivo imaging of fluorescent albumin modified with pyruvylated-human-type complex oligosaccharide reveals sialylation-like biodistribution and kinetics. International journal

    Ryoichiro Fukuhara, Akihiro Ogura, Sho Yoshinaga, Takamasa Fukunaga, Takashi Kinoshita, Wataru Sumiyoshi, Yujiro Higuchi, Katsunori Tanaka, Kaoru Takegawa

    Bioorganic & medicinal chemistry   70   116943 - 116943   2022.9   ISSN:0968-0896 eISSN:1464-3391

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    Both pyruvylation and sialylation onto the terminus of oligosaccharides of N-glycoproteins seem to be structurally and functionally similar with a property of conferring negative charge. However, detailed molecular characteristics of pyruvylation and sialylation in vivo were elusive. Here, to investigate an effect of terminal pyruvylation to N-glycan on in vivo biodistribution and kinetics, we prepared human serum albumin (HSA) modified with pyruvylated N-glycan (PvG), conjugated with HiLyte Fluor 750 (FL750-PvGHSA). In vivo imaging by using FL750-PvGHSA revealed that terminally pyruvylated N-glycoalbumin was excreted like sialylated N-glycoalbumin, suggesting that pyruvylation mimics sialylation in in vivo biodistribution and kinetics of N-glycoproteins. Terminal pyruvylation onto N-glycans can be a potential tool for a novel glycoengineering strategy.

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  • In vivo imaging of fluorescent albumin modified with pyruvylated-human-type complex oligosaccharide reveals sialylation-like biodistribution and kinetics. Reviewed International journal

    #Fukuhara R, Ogura A, Yoshinaga S, Fukunaga T, Kinoshita T, Sumiyoshi W, Higuchi Y, Tanaka K, Takegawa K.

    Bioorganic & Medicinal Chemistry   2022.9

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    DOI: 10.1016/j.bmc.2022.116943.

  • Characterization of novel endo-β-N-acetylglucosaminidase from Bacteroides nordii that hydrolyzes multi-branched complex type N-glycans.

    Kristina Mae Bienes, Feunai Agape Papalii Tautau, Ai Mitani, Takashi Kinoshita, Shin-Ichi Nakakita, Yujiro Higuchi, Kaoru Takegawa

    Journal of bioscience and bioengineering   134 ( 1 )   7 - 13   2022.7   ISSN:1389-1723 eISSN:1347-4421

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    Endo-β-N-acetylglucosaminidases (ENGases) are enzymes that hydrolyze the N-linked oligosaccharides. Many ENGases have already been identified and characterized. However, there are still a few enzymes that have hydrolytic activity toward multibranched complex-type N-glycans on glycoproteins. In this study, one novel ENGase from Bacteroides nordii (Endo-BN) species was identified and characterized. The recombinant protein was prepared and expressed in Escherichia coli cells. This Endo-BN exhibited optimum hydrolytic activity at pH 4.0. High performance liquid chromatography (HPLC) analysis showed that Endo-BN preferred core-fucosylated complex-type N-glycans, with galactose or α2,6-linked sialic acid residues at their non-reducing ends. The hydrolytic activities of Endo-BN were also tested on different glycoproteins from high-mannose type to complex-type oligosaccharides. The reaction with human transferrin, fetuin, and α1-acid glycoprotein subsequently showed that Endo-BN is capable of releasing multi-branched complex-type N-glycans from these glycoproteins.

    DOI: 10.1016/j.jbiosc.2022.03.011

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  • Characterization of novel endo-β-N-acetylglucosaminidase from Bacteroides nordii that hydrolyzes multi-branched complex type N-glycans. Reviewed

    2022.5

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  • SIN-like pathway kinases regulate the end of mitosis in the methylotrophic yeast Ogataea polymorpha. Reviewed International journal

    Maekawa H, Jiangyan S, Takegawa K, Pereira G

    Cells   2022.5

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    DOI: doi.org/10.3390/cells11091519

  • SIN-Like Pathway Kinases Regulate the End of Mitosis in the Methylotrophic Yeast <i>Ogataea polymorpha</i>

    Maekawa, H; Jiangyan, S; Takegawa, K; Pereira, G

    CELLS   11 ( 9 )   2022.5   eISSN:2073-4409

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    The mitotic exit network (MEN) is a conserved signalling pathway essential for the termination of mitosis in the budding yeast Saccharomyces cerevisiae. All MEN components are highly conserved in the methylotrophic budding yeast Ogataea polymorpha, except for Cdc15 kinase. Instead, we identified two essential kinases OpHcd1 and OpHcd2 (homologue candidate of ScCdc15) that are homologous to SpSid1 and SpCdc7, respectively, components of the septation initiation network (SIN) of the fission yeast Schizosaccharomyces pombe. Conditional mutants for OpHCD1 and OpHCD2 exhibited significant delay in late anaphase and defective cell separation, suggesting that both genes have roles in mitotic exit and cytokinesis. Unlike Cdc15 in S. cerevisiae, the association of OpHcd1 and OpHcd2 with the yeast centrosomes (named spindle pole bodies, SPBs) is restricted to the SPB in the mother cell body. SPB localisation of OpHcd2 is regulated by the status of OpTem1 GTPase, while OpHcd1 requires the polo-like kinase OpCdc5 as well as active Tem1 to ensure the coordination of mitotic exit (ME) signalling and cell cycle progression. Our study suggests that the divergence of molecular mechanisms to control the ME-signalling pathway as well as the loss of Sid1/Hcd1 kinase in the MEN occurred relatively recently during the evolution of budding yeast.

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  • Involvement of AAA ATPase AipA in endocytosis of the arginine permease AoCan1 depending on AoAbp1 in Aspergillus oryzae

    Hiasa Reiko, Kakimoto Ken-ichi, Takegawa Kaoru, Higuchi Yujiro

    Fungal Biology   126 ( 2 )   149 - 161   2022.2   ISSN:18786146 eISSN:1878-6162

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    AAA ATPases widely exist in many organisms and function in various organelles. However, there is little information about AAA ATPase functioning in endocytosis. In Aspergillus oryzae, we previously discovered a putative AAA ATPase AipA thatwould be involved in endocytosis. Here,we further examined the function of AipA and AoAbp1 in endocytosis using enhanced green fluorescent protein (EGFP)-tagged arginine permease AoCan1 as an endocytic marker. In the ΔaipA strain, endocytosis ofAoCan1-EGFPwasmore facilitated than the control strain, suggesting that AipA negatively regulates endocytosis. In contrast, in the ΔAoabp1 strain, endocytosis of AoCan1-EGFP was delayed compared with the control strain, suggesting that AoAbp1 positively functions in endocytosis. In addition, in the ΔaipAΔAoabp1 strain, endocytosis of AoCan1-EGFP was delayed. AipA localized at the endocytic collar of the hyphal tip, only in the presence of AoAbp1, suggesting AipA functions downstream of AoAbp1 in endocytosis. Moreover, we investigated the aipA-overexpressing strain, and found that endocytosis of AoCan1-EGFP was inhibited. Furthermore, we examined strains expressing <aipA>^^^K542A or <aipA>^^^E596Q,which decreased ATPase activity, in the backgrounds of complementation or overexpression, respectively, and found that AoCan1-EGFP endocytosis was promoted. These results suggested that AAA ATPase activity of AipA is important for its function in endocytosis.

    DOI: 10.1016/j.funbio.2021.11.007

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    CiNii Research

  • Involvement of AAA ATPase AipA in endocytosis depending on AoAbp1 in Aspergillus oryzae. Reviewed International journal

    #Hiasa R, #Kakimoto K, Takegawa K, Higuchi Y

    Fungal Biology   2022.1

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    DOI: doi: 10.1016/j.funbio.2021.11.007.

  • Characterization of novel endo-β-N-acetylglucosaminidase from Bacteroides nordii that hydrolyzes multi-branched complex type N-glycans. Reviewed

    竹川 薫

    Journal of Biosciemce and Bioengineering   133   2022

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  • Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe Reviewed

    Fukunaga T., Sakurai Y., Ohashi T., Higuchi Y., Maekawa H., Takegawa K.

    Applied Microbiology and Biotechnology   105 ( 23 )   8771 - 8781   2021.12   ISSN:01757598

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    Abstract: The glycoproteins of yeast contain a large outer chain on N-linked oligosaccharides; therefore, yeast is not suitable for producing therapeutic glycoproteins for human use. Using a deletion mutant strain of α1,6-mannosyltransferase (och1Δ), we previously produced humanized N-glycans in fission yeast; however, the Schizosaccharomyces pombe och1Δ cells displayed a growth delay even during vegetative growth, resulting in reduced productivity of heterologous proteins. To overcome this problem, here we performed a genome-wide screen for genes that would suppress the growth defect of temperature-sensitive och1Δ cells. Using a genomic library coupled with screening of 18,000 transformants, we identified two genes (pwp1+, SPBC1E8.05), both encoding GPI-anchored proteins, that increased the growth rate of och1Δ cells, lacking the outer chain. We further showed that a high copy number of the genes was needed to improve the growth rate. Mutational analysis of Pwp1p revealed that the GPI-anchored region of Pwp1p is important in attenuating the growth defect. Analysis of disruptants of pwp1+ and SPBC1E8.05 showed that neither gene was essential for cell viability; however, both mutants were sensitive β-glucanase, suggesting that Pwp1p and the protein encoded by SPBC1E8.05 non-enzymatically support β-glucan on the cell-surface of S. pombe. Collectively, our work not only sheds light on the functional relationships between GPI-anchored proteins and N-linked oligosaccharides of glycoproteins in S. pombe, but also supports the application of S. pombe to the production of human glycoprotein. Key points: • We screened for genes that suppress the growth defect of fission yeast och1Δ cells. • Appropriate expression of GPI-anchored proteins alleviates the growth delay of och1Δ cells. • The GPI-anchor domain of Pwp1p is important for suppressing the growth defect of och1Δ cells.

    DOI: 10.1007/s00253-021-11649-5

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  • Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe. Reviewed International journal

    #Fukunaga T, Sakurai Y, Ohashi T, Higuchi Y, Maekawa H, Takegawa K

    Applied Microbiology and Biotechnology   2021.11

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    DOI: doi: 10.1007/s00253-021-11649-5.

  • Correlative localization analysis between mRNA and EGFP-fused protein by single-molecule FISH using an egfp prove in Aspergillus oryzae. Reviewed International journal

    #Morita Y, Katakura Y, Takegawa K, Higuchi Y

    Frontiers in Fungal Biology   2021.10

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    DOI: doi: 10.3389/ffunb.2021.72139

  • Substrate specificities of α1,2- and α1,3-galactosyltransferases and the order of galactosylation in Schizosaccharomyces pombe. Reviewed International journal

    #Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K

    2021.6

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    DOI: doi: 10.1093/glycob/cwab028

  • The fission yeast gmn2+ gene encodes an ERD1 homologue of Saccharomyces cerevisiae required for protein glycosylation and retention of luminal endoplasmic reticulum proteins. Invited Reviewed International journal

    Tanaka N, Kagami A, Hirai K, Suzuki S, Matsuura S, #Fukunaga T, Tabuchi M, Takegawa K.

    The Journal of General and Applied Microbiology   2021.3

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    DOI: doi: 10.2323/jgam.2020.07.002.

  • Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe. Reviewed International journal

    Kawano-Kawada M, Ueda T, #Mori H, Ichimura H, Takegawa K, Sekito T

    Biochimica et Biophysica Acta-Biomembranes   2021.2

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    DOI: doi: 10.1016/j.bbamem.2020.183507.

  • Identification and characterization of β-D-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus. Invited Reviewed International journal

    2021.1

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    DOI: 10.1016/j.jbiosc.2020.09.006.

  • Identification and characterization of β-D-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus Reviewed

    Matsunaga E., Tanaka Y., Toyota S., Yamada H., Oka T., Higuchi Y., Takegawa K.

    Journal of Bioscience and Bioengineering   131 ( 1 )   1 - 7   2021.1   ISSN:13891723

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    Although β-D-galactofuranosidases (Galf-ases) that hydrolyze β-D-galactofuranose (Galf)-containing oligosaccharides have been characterized in various organisms, to date no Galf-specific Galf-ase-encoding genes have been reported in Aspergillus fungi. Based on the amino acid sequences of previously identified bacterial Galf-ases, here we found two candidate Galf-specific Galf-ase genes AN2395 (gfgA) and AN3200 (gfgB) in the genome of Aspergillus nidulans. Indeed, recombinant GfgA and GfgB proteins exhibited Galf-specific Galf-ase activity, but no detectable α-L-arabinofuranosidase (Araf-ase) activity. Phylogenetic analysis of GfgA and GfgB orthologs indicated that there are two types of Aspergillus species: those containing one ortholog each for GfgA and GfgB; and those containing only one ortholog in total, among which Aspergillus fumigatus there is a representative with a single ortholog Galf-ase Afu2g14520. Unlike GfgA and GfgB, the recombinant Afu2g14520 protein showed higher Araf-ase activity than Galf-ase activity. An assay of substrate specificity revealed that although GfgA and GfgB are both exo-type Galf-ases and hydrolyze β-(1,5) and β-(1,6) linkages, GfgA hydrolyzes β-(1,6)-linked Galf-oligosaccharide more effectively as compared with GfgB. Collectively, our findings indicate that Galf-ases in Aspergillus species may have a role in cooperatively degrading Galf-containing oligosaccharides depending on environmental conditions.

    DOI: 10.1016/j.jbiosc.2020.09.006

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  • Enzyme assay of glycoproteins by endo-β-N-acetylglucosaminidases.

    Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, C. de los Reyes DM, Bienes KM, Takegawa K

    2021

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    PubMed

  • Correlative Localization Analysis Between mRNA and Enhanced Green Fluorescence Protein-Fused Protein by a Single-Molecule Fluorescence in situ Hybridization Using an egfp Probe in Aspergillus oryzae Reviewed

    Morita Y., Katakura Y., Takegawa K., Higuchi Y.

    Frontiers in Fungal Biology   2   2021

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    Although subcellular localization analysis of proteins fused with enhanced green fluorescence protein (EGFP) has been widely conducted in filamentous fungi, little is known about the localization of messenger RNAs (mRNAs) encoding the EGFP-fused proteins. In this study, we performed single-molecule fluorescence in situ hybridization (smFISH) using an egfp probe to simultaneously visualize EGFP-fused proteins and their mRNAs in the hyphal cells of the filamentous fungus Aspergillus oryzae. We investigated the subcellular localization of mRNAs encoding cytoplasmic EGFP, an actin marker protein Lifeact tagged with EGFP, and several EGFP-fused proteins AoSec22, AoSnc1, AoVam3, and AoUapC that localize to the endoplasmic reticulum (ER), the apical vesicle cluster Spitzenkörper, vacuolar membrane, and plasma membrane, respectively. Visualization of these mRNAs by smFISH demonstrated that each mRNA exhibited distinct localization patterns likely depending on the mRNA sequence. In particular, we revealed that mRNAs encoding Lifeact-EGFP, EGFP-AoSec22, EGFP-AoVam3, and AoUapC-EGFP, but not cytoplasmic EGFP and EGFP-AoSnc1, were preferentially localized at the apical cell, suggesting certain mechanisms to regulate the existence of these transcripts among hyphal regions. Our findings provide the distinct localization information of each mRNA in the hyphal cells of A. oryzae.

    DOI: 10.3389/ffunb.2021.721398

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  • Golgi localization of glycosyltransferases requires Gpp74p in Schizosaccharomyces pombe Reviewed

    Ohashi T., Hegi S., Fukunaga T., Hosomi A., Takegawa K.

    Applied Microbiology and Biotechnology   104 ( 20 )   8897 - 8909   2020.10   ISSN:01757598

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    Abstract: The majority of Golgi glycosyltransferases are type II membrane proteins with a small cytosolic tail at their N-terminus. Several mechanisms for localizing these glycosyltransferases to the Golgi have been proposed. In Saccharomyces cerevisiae, the phosphatidylinositol-4-phosphate-binding protein ScVps74p interacts with the cytosolic tail of a Golgi glycosyltransferase and contributes to its localization. In this study, we investigated whether a similar mechanism functions in the fission yeast Schizosaccharomyces pombe. First, we identified gpp74+ (GPP34 domain-containing Vps74 homolog protein), a gene encoding the S. pombe homolog of S. cerevisiae Vps74p. Deletion of the gpp74+ gene resulted in the missorting of three Golgi glycosyltransferases, SpOch1p, SpMnn9p, and SpOmh1p, to vacuoles, but not SpAnp1p, indicating Gpp74p is required for targeting some glycosyltransferases to the Golgi apparatus. Gpp74p with an N-terminal GFP-tag localized to both the Golgi apparatus and the cytosol. Golgi localization of Gpp74p was dependent on the phosphatidylinositol 4-kinase SpPik1p. Site-directed mutagenesis of hydrophobic and basic amino acids in the cytosolic tails of SpOch1p and SpMnn9p resulted in their missorting to vacuoles, indicating these cytosolic N-terminal residues are important for localization in the Golgi. Unexpectedly, no prominent alternations in protein glycosylation were observed in S. pombe gpp74Δ cells, probably due to the residual Golgi localization of some SpOch1p and SpMnn9p in these cells. Collectively, these results demonstrate that both Gpp74p-dependent and Gpp74p-independent mechanisms are responsible for the Golgi localization of glycosyltransferases to the Golgi in S. pombe. Key points: • Gpp74p is involved in the localization of glycosyltransferases to the Golgi. • The cytosolic tails of glycosyltransferases are important for Golgi localization. • Gpp74p localizes to the Golgi in a SpPik1p-dependent manner.

    DOI: 10.1007/s00253-020-10881-9

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  • Golgi localization of glycosyltransferase requires Gpp74p in Schizosaccharomyces pombe. Invited Reviewed International journal

    Ohashi T, Hegi S, #Fukunaga T, Hosomi A, Takegawa K

    Applied Microbiology and Biotechnology   2020.10

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  • Single-molecule FISH reveals subcellular localization of α-amylase and actin mRNAs in the filamentous fungus Aspergillus oryzae. Invited Reviewed International journal

    2020.10

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    DOI: 10.3389/fmicb.2020.578862

  • Characterization and functional analysis of ERAD-related AAA+ ATPase Cdc48 in Aspergillus oryzae. Invited Reviewed International journal

    #Morita Y, #Kikumatsu F, Higuchi Y, Katakura Y, Takegawa K

    Fungal Biology   124 ( 9 )   801 - 813   2020.9   ISSN:18786146

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    DOI: 10.1016/j.funbio.2020.06.004

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  • SpMnn9p and SpAnp1p form protein complex involved in mannan backbone synthesis in the fission yeast Schizosaccharomyces pombe. Reviewed

    Ohashi T, Tanaka T, Tanaka N, Takegawa K.

    Journal of Bioscience and Bioengineering   2020.8

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    DOI: 10.1016/j.jbiosc.2020.06.003.

  • Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species Reviewed

    Fukunaga T., Tanaka N., Furumoto T., Nakakita S., Ohashi T., Higuchi Y., Maekawa H., Takegawa K.

    Journal of Bioscience and Bioengineering   130 ( 2 )   128 - 136   2020.8   ISSN:13891723

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    The N- and O-linked oligosaccharides from fission yeast Schizosaccharomyces pombe not only contain large amounts of D-mannose (Man) but also contain large amounts of D-galactose (Gal). Although the galactomannans of S. pombe are mainly composed of α1,2- or α1,3-linked Gals, some of the terminal α1,2-linked Gals are found to be linked to pyruvylated β1,3-linked galactose (PvGal). We have determined the structural characteristics of the N-glycans and O-glycans in three Schizosaccharomyces species (S. japonicus, S. octosporus, and S. cryophilus) using lectin blot, 1H NMR spectroscopy, and size-fractionation high performance liquid chromatography (HPLC), and found that the galactosylation of oligosaccharides was a common feature in fission yeasts. In addition, each of the terminal Galα1,2-, Galβ1,3- and non-substituted Man residues exhibited distinct characteristics. A BLAST search of gene databases in Schizosaccharomyces identified genes homologous to pvg1 encoding pyruvyltransferase of S. pombe. These genes, when expressed in an S. pombe pvg1Δ strains, led to the pyruvylation of non-reducing terminal β-linked Gal, suggesting the biosynthetic pathway of PvGal-containing oligosaccharides is highly conserved in fission yeasts.

    DOI: 10.1016/j.jbiosc.2020.03.008

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  • Microbial α-L-rhamnosidases show a dual L-rhamnose and L-mannose hydrolyzing activity. Reviewed

    2020.7

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    DOI: 10.5458/jag.jag.JAG-2020_0005

  • Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species. Reviewed International journal

    #Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K

    Journal of Bioscience and Bioengineering   2020.4

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    DOI: 10.1016/j.jbiosc.2020.03.008.

  • Identification and characterization of a novel, versatile sialidase from a Sphingobacterium that can hydrolyze the glycosides of any sialic acid species at neutral pH Reviewed

    Iwaki Y., Matsunaga E., Takegawa K., Sato C., Kitajima K.

    Biochemical and Biophysical Research Communications   523 ( 2 )   487 - 492   2020.3   ISSN:0006291X

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    Bacterial sialidases are widely used to remove sialic acid (Sia) residues from glycans. Most of them cleave the glycosides of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) under acidic pHs; however, currently available bacterial sialidases had no activity to the glycosides of deaminoneuraminic acid (Kdn). In this study, we found a novel sialidase from Sphingobacterium sp. strain HMA12 that could cleave any of the glycosides of Neu5Ac, Neu5Gc, and Kdn. It also had a broad linkage specificity, i.e., α2,3-, α2,6-, α2,8-, and α2,9-linkages, and the optimal pH at neutral ranges, pH 6.5–7.0. These properties are particularly important when sialidases are applied for in vivo digestion of the cell surface sialosides under physiological conditions. Interestingly, 2,3-didehydro-2-deoxy-N-acetylneuraminic acid (Neu5Ac2en), which is a transition state analog-based inhibitor, competitively inhibited the enzyme-catalyzed reaction for Kdn as well as for Neu5Ac, suggesting that the active site is common to the Neu5Ac and Kdn residues. Taken together, this sialidase is versatile and useful for the in vivo research on sialo-glycoconjugates.

    DOI: 10.1016/j.bbrc.2019.12.079

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  • Identification and characterization of a novel, versatile sialidase from a Sphingobacterium that can hydrolyze the glycosides of any sialic acid species at neutral pH. Reviewed International journal

    Iwaki Y, Matsunaga E, Takegawa K, Sato C, Kitajima K

    Biochemical and Biophysical Research Communications   2020.3

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    DOI: 10.1016/j.bbrc.2019.12.079.

  • Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae. Reviewed

    Li Q, Higuchi Y, Tanabe K, Katakura Y, Takegawa K

    Journal of Bioscience and Bioengineering   2020.2

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    DOI: 10.1016/j.jbiosc.2019.12.006.

  • The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan. Reviewed International journal

    Shen L, Viljoen A, Villaume S, Maju J, Chene L, Mery A, Fabre E, Takegawa K, Lowary TL, Vincent SP, Kremer L, Guerardel Y, Mariller C

    Journal of Biological Chemistry   2020.2

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    DOI: 10.1074/jbc.RA119.011817.

  • Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and o-mannose-type galactomannans within the invasive pathogen aspergillus fumigatus Reviewed

    Chihara Y., Tanaka Y., Izumi M., Hagiwara D., Watanabe A., Takegawa K., Kamei K., Shibata N., Ohta K., Oka T.

    mSphere   5 ( 1 )   2020.1

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    ABSTRACT The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes. Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/ β-(1→6)-galactofuranosyl chains. We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains. In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus. Two paralogs exist within A. fumigatus: GfsB and GfsC. We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses. GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity. Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus. The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice. IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota. Pezizomycotina includes many plant and animal pathogens. Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan. Fungal cell wall formation processes are complicated, and information concerning glycosyltransferases is essential for understanding them. In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans. The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity. Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.

    DOI: 10.1128/mSphere.0770-19

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  • Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Gal-actomannans within the Invasive Pathogen Aspergillus fumigatus. Reviewed International journal

    2020.1

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  • 1,6-alpha-Fucosidases from Bifidobacterium longum subsp. Infantis ATCC15697 involved in the degradation of core-fucosylated N-glycan. Reviewed

    Ashida H, Fujimoto T, Kurihara S, Nakamura M, Komeno M, #Huang Y, Katayama T, Kinoshita T, Takegawa K

    Journal of Applied Glycoiscience   2020.1

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  • Galactofuranosidase from JHA 19 Streptomyces sp.: subcloning and biochemical characterization Reviewed

    Seničar M., Legentil L., Ferrières V., Eliseeva S.V., Petoud S., Takegawa K., Lafite P., Daniellou R.

    Carbohydrate Research   480   35 - 41   2019.7   ISSN:00086215

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    Despite the crucial role of the rare galactofuranose (Galf) in many pathogenic micro-organisms and our increased knowledge of its metabolism, there is still a lack of recombinant and efficient galactofuranoside hydrolase available for chemo-enzymatic synthetic purposes of specific galactofuranosyl-conjugates. Subcloning of the Galf-ase from JHA 19 Streptomyces sp. and its further overexpression lead us to the production of this enzyme with a yield of 0.5 mg/L of culture. It exhibits substrate specificity exclusively towards pNP β-D-Galf, giving a KM value of 250 μM, and the highest enzymatic efficiency ever observed of 14 mM−1 s−1. It proved to be stable to temperature up to 60 °C and to at least 4 freeze-thaw's cycles. Thus, Galf-ase demonstrated to be an efficient and stable biocatalyst with greatly improved specificity toward the galactofuranosyl entity, thus paving the way to the further development of transglycosylation and thioligation reactions.

    DOI: 10.1016/j.carres.2019.05.011

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  • Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response factors to alkaline and salt stress Reviewed

    Tominaga A., Higuchi Y., Mori H., Akai M., Suyama A., Yamada N., Takegawa K.

    Applied Microbiology and Biotechnology   103 ( 12 )   4881 - 4887   2019.6   ISSN:01757598

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    How cells of the fission yeast Schizosaccharomyces pombe respond to alkaline stress is not well understood. Here, to elucidate the molecular mechanism underlying the alkaline stress response in S. pombe, we performed DNA microarray analysis. We found that a homolog of human catechol O-methyltransferase 2 (COMT2) is highly upregulated in S. pombe cells exposed to alkaline conditions. We designated the S. pombe homolog as cmt2+ and also identified its paralog, cmt1+, in the S. pombe genome. Reverse transcription PCR confirmed that both cmt1+ and cmt2+ are upregulated within 1 h of exposure to alkaline stress and downregulated within 30 min of returning to an acidic environment. Moreover, we verified that recombinant Cmt proteins exhibit catechol O-methyltransferase activity. To further characterize the expression of cmt1+ and cmt2+, we carried out an EGFP reporter assay using their promoter sequences, which showed that both genes respond not only to alkaline but also to salt stress. Collectively, our findings indicate that the cmt promoter might be an advantageous expression system for use in S. pombe under alkaline culture conditions.

    DOI: 10.1007/s00253-019-09858-0

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  • Galactofuranosidase from JHA19 Streptomyces sp.: subcloning and biochemical characteriza-tion. Reviewed International journal

    Senicar M, Legentil L, Ferrieres V, Eliseeva SV, Petoud S, Takegawa K, Lafite P, Daniellou R

    Carbohydrate Research   2019.6

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    DOI: 10.1016/j.carres.2019.05.011.

  • Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response fac-tors to alkaline and salt stress. Reviewed International journal

    #Tominaga A, Higuchi Y, #Mori H, #Akai M, Suyama A, Yamada N, Takegawa K

    Applied Microbiology and Biotechnology   2019.5

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    DOI: 10.1007/s00253-019-09858-0.

  • Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan Reviewed

    Ota R., Okamoto Y., Vavricka C., Oka T., Matsunaga E., Takegawa K., Kiyota H., Izumi M.

    Carbohydrate Research   473   99 - 103   2019.2   ISSN:00086215

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    β-D-Galactofuranose (Galf) is a component of polysaccharides and glycoconjugates. There are few reports about the involvement of galactofuranosyltransferases and galactofuranosidases (Galf-ases) in the synthesis and degradation of galactofuranose-containing glycans. The cell walls of filamentous fungi in the genus Aspergillus include galactofuranose-containing polysaccharides and glycoconjugates, such as O-glycans, N-glycans, and fungal-type galactomannan, which are important for cell wall integrity. In this study, we investigated the synthesis of p-nitrophenyl β-D-galactofuranoside and its disaccharides by chemo-enzymatic methods including use of galactosidase. The key step was selective removal of the concomitant pyranoside by enzymatic hydrolysis to purify p-nitrophenyl β-D-galactofuranoside, a promising substrate for β-D-galactofuranosidase from Streptomyces species.

    DOI: 10.1016/j.carres.2019.01.005

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  • Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan Reviewed International journal

    2019.2

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  • 微量香気成分インドールが大麦焼酎の官能特性に及ぼす影響 Reviewed

    梶原康博、大石雅志、竹川 薫、高下秀春

    日本醸造協会誌   2019.1

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  • Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG Reviewed

    Huang Y., Higuchi Y., Kinoshita T., Mitani A., Eshima Y., Takegawa K.

    Scientific Reports   8 ( 1 )   2018.12

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    Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis. By draft genome sequencing, five candidate ENGase encoding genes were identified in the genome of this strain. Recombinant proteins, purified from Escherichia coli expressing candidate genes ORF1152, ORF1188, ORF3046 and ORF3750 exhibited fucose-containing oligosaccharides-specific ENGase activity. These ENGases exhibited optimum activities at very acidic pHs (between pH 2.3-2.5). BLAST searches using sequences of these candidate genes identified two fungal homologs of ORF1188, one in Beauveria bassiana and the other in Cordyceps militaris. Recombinant ORF1188, Beauveria and Cordyceps ENGases released the fucose-containing oligosaccharides residues from rituximab (immunoglobulin G) but not the high-mannose-containing oligosaccharides residues from RNase B, a result that not only confirmed the substrate specificity of these novel ENGases but also suggested that natural glycoproteins could be their substrates.

    DOI: 10.1038/s41598-017-17467-y

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  • Draft genome sequence of Bacillus sp. HMA207, a strain that exhibits β-D-galactosidase activ-ity to release pyruvylated galactose. Reviewed International journal

    Higuchi Y., Matsufuji H., Mori K., Matsunaga E., Tashiro K., Takegawa K.

    Microbiology Resource Announcements   7 ( 10 )   2018.9

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    The genome sequence of the Bacillus sp. strain HMA207, the culture supernatant of which exhibited -D-galactosidase activity to release pyruvylated galactose (PvGal), was examined to identify a PvGal-ase-encoding gene. We report here the result of whole-genome shotgun sequencing, which revealed putative PvGal-ase genes.

    DOI: 10.1128/MRA.01169-18

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  • Substrate specificity of Nudix hydrolases from Myxococcus xanthus. Reviewed

    Kimura Y, Yamamoto Y, Kajimoto S, Sakai A, Takegawa K

    Journal of General and Applied Microbiology   2018.5

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    DOI: 10.2323/jgam.2017.07.001

  • Catalytic Activity Profile of Polyphosphate Kinase 1 from Myxococcus xanthus Reviewed

    Kamatani S., Takegawa K., Kimura Y.

    Current Microbiology   75 ( 4 )   379 - 385   2018.4   ISSN:03438651

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    Polyphosphate kinase 1 (Ppk1) catalyzes reverse transfer of the terminal phosphate from ATP to form polyphosphate (polyP) and from polyP to form ATP, and is responsible for the synthesis of most of cellular polyPs. When Ppk1 from Myxococcus xanthus was incubated with 0.2 mM polyP60−70 and 1 mM ATP or ADP, the rate of ATP synthesis was approximately 1.5-fold higher than that of polyP synthesis. If in the same reaction the proportion of ADP in the ATP/ADP mixture exceeded one-third, the equilibrium shifted to ATP synthesis, suggesting that M. xanthus Ppk1 preferentially catalyzed ATP formation. At the same time, GTP and GDP were not recognized as substrates by Ppk1. In the absence of polyP, Ppk1 generated ATP and AMP from ADP, and ADP from ATP and AMP, suggesting that the enzyme catalyzed the transfer of a phosphate group between ADP molecules yielding ATP and AMP, thus exhibiting adenylate kinase activity.

    DOI: 10.1007/s00284-017-1391-y

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  • Catalytic activity profile of polyphosphate kinase 1 from Myxococcus xanthus. Reviewed International journal

    Kamatani S, Takegawa K, Kimura Y

    Current Microbiology   2018.4

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    DOI: 10.1007/s00284-017-1391-y.

  • Genomic sequence of Saccharomyces cerevisiae BAW-6, a yeast strain opt imal for brewing barley shochu. Reviewed International journal

    Kajiwara Y, Mori K, Tashiro K, Higuchi Y, Takegawa K, Takashita H

    Genome Announcements   6 ( 14 )   2018.4

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    DOI: 10.1128/genomeA.00228-18

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  • Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1 Reviewed

    Sugihara A., Nguyen L.C., Shamim H.M., Iida T., Nakase M., Takegawa K., Senda M., Jida S., Ueno M.

    Biochemical and Biophysical Research Communications   496 ( 4 )   1284 - 1290   2018.2   ISSN:0006291X

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    Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.

    DOI: 10.1016/j.bbrc.2018.02.001

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  • Draft genome sequence of Sphingobacterium sp. strain HMA12, which encodes endo-β-Nacetylglucosaminidases and can specifically hydrolyze fucose-containing oligosaccharides Reviewed

    Huang Y., Higuchi Y., Mori K., Yamashita R., Okino N., Tashiro K., Takegawa K.

    Genome Announcements   6 ( 8 )   2018.2

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    The genome sequence of the soil bacterium Sphingobacterium sp. strain HMA12, the culture supernatant of which exhibited endo-β-N-acetylglucosaminidase (ENGase) activity, was examined for ENGase-encoding genes. Here, we report the characterization of new genes of ENGases, obtained by whole-genome shotgun sequencing, that are capable of specifically hydrolyzing fucose-containing oligosaccharides.

    DOI: 10.1128/genomeA.01525-17

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  • Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1. Reviewed International journal

    Sugiura A, Nguyen LC, Shamim HM, Iida N, Nakamura Y, Iida T, Nakase M, Takegawa K, Senda M, Jida S, Ueno M

    Biochemistry, Biophysics and Research Communications   2018.2

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    DOI: 10.1016/j.bbrc.2018.02.001.

  • Draft genome sequence of Sphingobacterium sp. HMA12, a strain that holds endo-β-N-acetylglucosaminidases specifically hydrolyzing fucose-containing oligosaccharides. Reviewed International journal

    2018.2

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    DOI: 10.1128/genomeA.01525-17.

  • Analysis of alkaline stress response mediated by iron and copper intake in Schizosaccharomyces pombe. Reviewed

    Yujiro Higuchi, Hikari Mori, Takeo Kubota, Takegawa K

    Journal of Bioscience and Bioengineering   2018.1

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    DOI: 10.1016/j.jbiosc.2017.08.008.

  • Analysis of ambient pH stress response mediated by iron and copper intake in Schizosaccharomyces pombe Reviewed

    Higuchi Y., Mori H., Kubota T., Takegawa K.

    Journal of Bioscience and Bioengineering   125 ( 1 )   92 - 96   2018.1   ISSN:13891723

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    The molecular mechanism of tolerance to alkaline pH is well studied in model fungi Aspergillus nidulans and Saccharomyces cerevisiae. However, how fission yeast Schizosaccharomyces pombe survives under alkaline stress remains largely unknown, as the genes involved in the alkaline stress response pathways of A. nidulans and S. cerevisiae were not found in the genome of this organism. Since uptake of iron and copper into cells is important for alkaline tolerance in S. cerevisiae, here we examined whether iron and copper uptake processes were involved in conferring tolerance to alkaline stress in S. pombe. We first revealed that S. pombe wild-type strain could not grow at a pH higher than 6.7. We further found that the growths of mutants harboring disruption in the iron uptake-related gene frp1+, fio1+ or fip1+ were severely inhibited under ambient pH stress condition. In contrast, derepression of these genes, by deletion of their repressor gene fep1+, caused cells to acquire resistance to pH stress. Together, these results suggested that uptake of iron is essential for ambient pH tolerance in S. pombe. We also found that copper is required for the pH stress response because disruptants of ctr4+, ctr5+, ccc2+ and cuf1+ genes, all of which are needed for regulating intracellular Cu+, displayed ambient pH sensitivity. Furthermore, supplementing Fe2+ and Cu2+ ions to the culture media improved growth under ambient pH stress. Taken together, our results suggested that uptake of iron and copper is the crucial factor needed for the adaptation of S. pombe to ambient pH stress.

    DOI: 10.1016/j.jbiosc.2017.08.008

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  • Regulation of mating type switching by the mating type genes and RME1 in Ogataea polymorpha Reviewed

    Yamamoto K., Tran T.N.M., Takegawa K., Kaneko Y., Maekawa H.

    Scientific Reports   7 ( 1 )   2017.12

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    Saccharomyces cerevisiae and its closely related yeasts undergo mating type switching by replacing DNA sequences at the active mating type locus (MAT) with one of two silent mating type cassettes. Recently, a novel mode of mating type switching was reported in methylotrophic yeast, including Ogataea polymorpha, which utilizes chromosomal recombination between inverted-repeat sequences flanking two MAT loci. The inversion is highly regulated and occurs only when two requirements are met: haploidy and nutritional starvation. However, links between this information and the mechanism associated with mating type switching are not understood. Here we investigated the roles of transcription factors involved in yeast sexual development, such as mating type genes and the conserved zinc finger protein Rme1. We found that co-presence of mating type a1 and α2 genes was sufficient to prevent mating type switching, suggesting that ploidy information resides solely in the mating type locus. Additionally, RME1 deletion resulted in a reduced rate of switching, and ectopic expression of O. polymorpha RME1 overrode the requirement for starvation to induce MAT inversion. These results suggested that mating type switching in O. polymorpha is likely regulated by two distinct transcriptional programs that are linked to the ploidy and transmission of the starvation signal.

    DOI: 10.1038/s41598-017-16284-7

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  • Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae Reviewed

    Togo Y., Higuchi Y., Katakura Y., Takegawa K.

    Scientific Reports   7 ( 1 )   2017.12

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    Recent research in filamentous fungi has revealed that the motility of an endocytic organelle early endosome (EE) has a versatile role in many physiological functions. Here, to further examine the motility of EEs in the industrially important fungus Aspergillus oryzae, we visualized these organelles via the Rab5 homolog AoRab5 and identified AoHok1, a putative linker protein between an EE and a motor protein. The Aohok1 disruptant showed retarded mycelial growth and no EE motility, in addition to an apical accumulation of EEs and peroxisomes. We further demonstrated that the Aohok1 disruptant exhibited less sensitivity to osmotic and cell wall stresses. Analyses on the protein secretory pathway in ΔAohok1 cells showed that, although distribution of the endoplasmic reticulum and Golgi was not affected, formation of the apical secretory vesicle cluster Spitzenkörper was impaired, probably resulting in the observed reduction of the A. oryzae major secretory protein α-amylase. Moreover, we revealed that the transcript level of α-amylase-encoding gene amyB was significantly reduced in the Aohok1 disruptant. Furthermore, we observed perturbed conidial and sclerotial formations, indicating a defect in cell differentiation, in the Aohok1 disruptant. Collectively, our results suggest that EE motility is crucial for α-amylase production and cell differentiation in A. oryzae.

    DOI: 10.1038/s41598-017-16163-1

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  • Preparation and biological activities of anti-HER2 monoclonal antibodies with fully core-fucosylated homogeneous bi-antennary complex-type glycans. Reviewed

    Tsukimura W, Kurogochi M, Mori M, Osumi K, Matsuda A, Takegawa K, Furukawa K, Shirai T

    Bioscience, Biotechnology, and Biochemistry   2017.12

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    DOI: 10.1016/j.jbiosc.2017.08.008.

  • Analysis of an acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion Reviewed

    Kwon H.S., Kawaguchi K., Kikuma T., Takegawa K., Kitamoto K., Higuchi Y.

    Biochemical and Biophysical Research Communications   493 ( 1 )   481 - 486   2017.11   ISSN:0006291X

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    Acyl-CoA binding protein (ACBP) plays important roles in the metabolism of lipids in eukaryotic cells. In the industrially important filamentous fungus Aspergillus oryzae, although we have previously demonstrated that the A. oryzae ACBP (AoACBP) localizes to punctate structures and exhibits long-range motility, which is dependent on autophagy-related proteins, the physiological role of AoACBP remains elusive. Here, we describe identification and characterization of another ACBP from A. oryzae; we named this ACBP as AoAcb2 and accordingly renamed AoACBP as AoAcb1. The deduced amino acid sequence of AoAcb2 lacked a signal peptide. Phylogenetic analysis classified AoAcb2 into a clade that was same as the ACBP Acb1 of the model yeast Saccharomyces cerevisiae, but was different from that of AoAcb1. In contrast to punctate localization of AoAcb1, AoAcb2 was found to be dispersedly distributed in the cytoplasm, as was previously observed for the S. cerevisiae Acb1. Since we could not generate an Aoacb2 disruptant, we created an Aoacb2 conditional mutant that exhibited less growth under Aoacb2-repressed condition, suggesting that Aoacb2 is an essential gene for growth. Moreover, we observed that A. oryzae AoAcb2, but not A. oryzae AoAcb1, was secreted under carbon-starved condition, suggesting that AoAcb2 might be secreted via the unconventional protein secretion (UPS) pathway, just like S. cerevisiae Acb1. We also demonstrated that the unconventional secretion of AoAcb2 was dependent on the t-SNARE AoSso1, but was independent of the autophagy-related protein AoAtg1, suggesting that the unconventional secretion of AoAcb2, unlike that of S. cerevisiae Acb1, via the UPS pathway, is not regulated by the autophagy machinery. Thus, the filamentous fungus A. oryzae harbors two types of ACBPs, one of which appears to be essential for growth and undergoes unconventional secretion.

    DOI: 10.1016/j.bbrc.2017.08.166

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  • Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae. Reviewed International journal

    2017.11

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    DOI: 10.1038/s41598-017-16163-1.

  • Regulation of the mating type switching by the mating type genes and RME1 in Ogataea polymorpha. Invited Reviewed International journal

    Yamamoto K, Tran T, Takegawa K, Kaneko Y, Maekawa H

    Scientific Reports   2017.11

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    DOI: 10.1038/s41598-017-16284-7.

  • Analysis of acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion. Reviewed International journal

    HeeSu Kwon, Kouhei Kawaguchi, Takashi Kukuma, Kaoru Takegawa, Katsuhiko Kitamoto, Yujiro Higuchi

    Biochemical and Biophysical Research Communications   2017.10

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  • Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant fission yeast strains. Reviewed

    Suyama A, Higuchi Y, Urushihara M, Maeda Y, Takegawa K

    Journal of Bioscience and Bioengineering   124 ( 4 )   392 - 399   2017.8   ISSN:13891723

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    DOI: 10.1016/j.jbiosc.2017.04.015

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  • Draft genome sequence of Streptomyces sp. JHA26, a strain that harbors a PA14 domain-containing β-D-galactofuranosidase Reviewed International journal

    Matsunaga E., Higuchi Y., Mori K., Tashiro K., Takegawa K.

    Genome Announcements   5 ( 15 )   2017.6

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    The genome sequence of Streptomyces sp. strain JHA26, the culture supernatant of which exhibited β-D-galactofuranosidase (Galf-ase) activity, was analyzed to search for a Galf-ase-encoding gene. We report here the results of wholegenome shotgun sequencing and reveal the identity of a new Galf-ase gene.

    DOI: 10.1128/genomeA.00190-17

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  • GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus Reviewed International journal

    Katafuchi Y., Li Q., Tanaka Y., Shinozuka S., Kawamitsu Y., Izumi M., Ekino K., Mizuki K., Takegawa K., Shibata N., Goto M., Nomura Y., Ohta K., Oka T.

    Glycobiology   27 ( 6 )   568 - 581   2017.6   ISSN:09596658

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    Previously, we reported that GfsA is a novel galactofuranosyltransferase involved in the biosynthesis of O-glycan, the proper maintenance of fungal morphology, the formation of conidia and anti-fungal resistance in Aspergillus nidulans and A. fumigatus (Komachi Y et al., 2013. GfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus. Mol. Microbiol. 90:1054-1073). In the present paper, to gain an in depth-understanding of the enzymatic functions of GfsA in A. fumigatus (AfGfsA), we established an in vitro assay to measure galactofuranosyltransferase activity using purified AfGfsA, UDP-α-D-galactofuranose as a sugar donor, and p-nitrophenyl-β-Dgalactofuranoside as an acceptor substrate. LC/MS, 1H-NMR and methylation analyses of the enzymatic products of AfGfsA revealed that this protein has the ability to transfer galactofuranose to the C-5 position of the β-galactofuranose residue via a β-linkage. AfGfsA requires a divalent cation of manganese for maximal activity and consumes UDP-α-D-galactofuranose as a sugar donor. Its optimal pH range is 6.5-7.5 and its optimal temperature range is 20-30°C. 1H-NMR, 13C-NMR and methylation analyses of fungal-type galactomannan extracted from the δAfgfsA strain revealed that AfGfsA is responsible for the biosynthesis of β1,5-galactofuranose in the galactofuran side chain of fungal-type galactomannan. Based on these results, we conclude that AfGfsA acts as a UDP-α-D-galactofuranose: β-D-galactofuranoside β1,5-galactofuranosyltransferase in the biosynthetic pathway of galactomannans.

    DOI: 10.1093/glycob/cwx028

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  • Characterization of PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp. Reviewed International journal

    2017.5

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    DOI: 10.1080/09168451.2017.1300518.

  • Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline Reviewed

    Higuchi Y., Eshima Y., Huang Y., Kinoshita T., Sumiyoshi W., Nakakita S., Takegawa K.

    Biotechnology Letters   39 ( 1 )   157 - 162   2017.1   ISSN:01415492

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    Objectives: To establish an efficient method of chemoenzymatic modification for making N-linked oligosaccharide chains of glycoproteins structurally homogeneous, which crucially affects their bioactivities. Results: Deglycosylated-RNase B (GlcNAc-RNase B; acceptor), sialylglyco (SG)-oxazoline (donor) and an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase (Endo-CCN180H) were employed. pH 7.5 was ideal for both SG-oxazoline’s stability and Endo-CC’s transglycosylation reaction. The most efficient reaction conditions for producing glycosylated-RNase B, virtually modified completely with sialo-biantennary-type complex oligosaccharide, were: 80 μg GlcNAc-RNase B, 200 μg SG-oxazoline and 3 μg Endo-CCN180H in 20 μl 20 mM Tris/HCl pH 7.5 at 30 °C for 30–60 min. Conclusions: This transglycosylation method using SG-oxazoline and Endo-CCN180H is beneficial for producing pharmaceutical glycoproteins modified with homogenous biantennary-complex-type oligosaccharides.

    DOI: 10.1007/s10529-016-2230-0

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  • Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline. Reviewed International journal

    Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K

    Biotechnology Letters   2017.1

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    DOI: 10.1016/j.bbrc.2016.10.018.

  • Preparation and biological activities of anti-HER2 monoclonal antibodies with fully core-fucosylated homogeneous bi-antennary complex-type glycans Reviewed

    Tsukimura W., Kurogochi M., Mori M., Osumi K., Matsuda A., Takegawa K., Furukawa K., Shirai T.

    Bioscience, Biotechnology and Biochemistry   81 ( 12 )   2353 - 2359   2017   ISSN:09168451

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    Recently, the absence of a core-fucose residue in the N-glycan has been implicated to be important for enhancing antibody-dependent cellular cytotoxicity (ADCC) activity of immunoglobulin G monoclonal antibodies (mAbs). Here, we first prepared anti-HER2 mAbs having two core-fucosylated N-glycan chains with the single G2F, G1aF, G1bF, or G0F structure, together with those having two N-glycan chains with a single non-core-fucosylated corresponding structure for comparison, and determined their biological activities. Dissociation constants of mAbs with core-fucosylated N-glycans bound to recombinant Fcγ-receptor type IIIa variant were 10 times higher than those with the non-core-fucosylated N-glycans, regardless of core glycan structures. mAbs with the core-fucosylated N-glycans had markedly reduced ADCC activities, while those with the non-core-fucosylated N-glycans had high activities. These results indicate that the presence of a core-fucose residue in the N-glycan suppresses the binding to the Fc-receptor and the induction of ADCC of anti-HER2 mAbs.

    DOI: 10.1080/09168451.2017.1394813

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  • Characterization of a PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp. Reviewed

    Matsunaga E., Higuchi Y., Mori K., Yairo N., Toyota S., Oka T., Tashiro K., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   81 ( 7 )   1314 - 1319   2017   ISSN:09168451

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    As a constituent of polysaccharides and glycoconjugates, β-D-galactofuranose (Galf) exists in several pathogenic microorganisms. Although we recently identified a β-D-galactofuranosidase (Galf-ase) gene, ORF1110, in the Streptomyces strain JHA19, very little is known about the Galf-ase gene. Here, we characterized a strain, named JHA26, in the culture supernatant of which exhibited Galf-ase activity for 4-nitrophenyl β-D-galactofuranoside (pNP-β-D-Galf) as a substrate. Draft genome sequencing of the JHA26 strain revealed a putative gene, termed ORF0643, that encodes Galf-ase containing a PA14 domain, which is thought to function in substrate recognition. The recombinant protein expressed in Escherichia coli showed the Galf-specific Galf-ase activity and also released galactose residue of the polysaccharide galactomannan prepared from Aspergillus fumigatus, suggesting that this enzyme is an exo-type Galf-ase. BLAST searches using the amino acid sequences of ORF0643 and ORF1110 Galf-ases revealed two types of Galf-ases in Acti-nobacteria, suggesting that Galf-specific Galf-ases may exhibit discrete substrate specificities.

    DOI: 10.1080/09168451.2017.1300518

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  • The amino-terminal hydrophilic region of the vacuolar transporter Avt3p is dispensable for the vacuolar amino acid compartmentalization of Schizosaccahromyces pombe. Invited Reviewed International journal

    Kawano-Kawada M, Chardwiriyapreecha S, Manabe K, Sekito T, Akiyama K, Takegawa K, Kakinuma Y

    Bioscience, Biotechnology and Biochemistry   2016.12

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  • Subcellular localization of acyl-CoA binding protein in Aspergillus oryzae is regulated by autophagy machinery. Invited Reviewed International journal

    Kawaguchi K, KikumaT, Yujiro Higuchi, Takegawa K, Kitamoto K

    Biochemical and Biophysical Research Communications   2016.12

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  • Lysyl-tRNA synthetase from Myxococcus xanthus catalyzes the formation of diadenosine penta- and hexaphosphates from adenosine tetraphosphate Reviewed

    Oka M., Takegawa K., Kimura Y.

    Archives of Biochemistry and Biophysics   604   152 - 158   2016.8   ISSN:00039861

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    Myxococcus xanthus lysyl-tRNA synthetase (LysS) produces diadenosine tetraphosphate (Ap4A) from ATP in the presence of Mn2+; in the present study, it also generated Ap4 from ATP and triphosphate. When ATP and Ap4 were incubated with LysS and pyrophosphatase, first Ap4A, Ap5A, and ADP, and then Ap5, Ap6A, and Ap3A were generated. The results suggest that in the first step, LysS can form lysyl-AMP and lysyl-ADP intermediates from Ap4 and release triphosphate and diphosphate, respectively, whereas in the second step, it can produce Ap5 from lysyl-ADP with triphosphate, and Ap6A from lysyl-ADP with Ap4. In addition, in the presence of Ap4 and pyrophosphatase, but absence of ATP, LysS also generates diadenosine oligophosphates (ApnAs: n = 3–6). These results indicate that LysS has the ability to catalyze the formation of various ApnAs from Ap4 in the presence of pyrophosphatase. Furthermore, the formation of Ap4A by LysS was inhibited by tRNALys in the presence of 1 mM ATP. To the best of our knowledge, this is the first report of Ap5A and Ap6A synthesis by lysyl-tRNA synthetase.

    DOI: 10.1016/j.abb.2016.07.002

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  • Lysyl-tRNA synthetase from Myxococcus xanthus catalyzes the formation of diadenosine penta- and hexaphsphates from denosine tetraphosphate. Invited Reviewed International journal

    Oka M, Takegawa K, Kimura Y

    Archives of Biochemistry and Biophysics   2016.8

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  • A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-Type complex oligosaccharide Reviewed

    Higuchi Y., Yoshinaga S., Yoritsune K., Tateno H., Hirabayashi J., Nakakita S., Kanekiyo M., Kakuta Y., Takegawa K.

    Scientific Reports   6   2016.5

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    Pyruvylation onto the terminus of oligosaccharide, widely seen from prokaryote to eukaryote, confers negative charges on the cell surface and seems to be functionally similar to sialylation, which is found at the end of human-Type complex oligosaccharide. However, detailed molecular mechanisms underlying pyruvylation have not been clarified well. Here, we first determined the crystal structure of fission yeast pyruvyltransferase Pvg1p at a resolution of 2.46 Å. Subsequently, by combining molecular modeling with mutational analysis of active site residues, we obtained a Pvg1p mutant (Pvg1p H168C) that efficiently transferred pyruvyl moiety onto a human-Type complex glycopeptide. The resultant pyruvylated human-Type complex glycopeptide recognized similar lectins on lectin arrays as the α2,6-sialyl glycopeptides. This newly-generated pyruvylation of human-Type complex oligosaccharides would provide a novel method for glyco-bioengineering.

    DOI: 10.1038/srep26349

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  • Draft genome sequence of Bacillus clausii AKU0647, a strain that produces endo-β-N-acetylglucosaminidase A Reviewed International journal

    2016.4

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  • Draft genome sequence of Bacillus clausii AKU0647, a strain that produces endo-β-Nacetylglucosaminidase A Reviewed

    Higuchi Y., Mori K., Suyama A., Huang Y., Tashiro K., Kuhara S., Takegawa K.

    Genome Announcements   4 ( 3 )   2016

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    To comprehensively identify glycosyl hydrolase genes in the genome of Bacillus clausii strain AKU0647, which produces endo- β-N-acetylglucosaminidase A (Endo-A), we conducted whole-genome shotgun sequencing. We identified several other putative glycosyl hydrolase genes apart from the Endo-A gene, and report these findings here.

    DOI: 10.1128/genomeA.00310-16

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  • Draft genome sequence of Streptomyces sp. JHA19, a strain that possesses β-D-galactofuranosidase activity. Reviewed International journal

    2015.10

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  • Identification and characterization of a novel galactofuranose-specific β-D-galactofuranosidase from Streptomyces species Reviewed

    Matsunaga E., Higuchi Y., Mori K., Yairo N., Oka T., Shinozuka S., Tashiro K., Izumi M., Kuhara S., Takegawa K.

    PLoS ONE   10 ( 9 )   2015.9

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    β-D-galactofuranose (Galf) is a component of polysaccharides and glycoconjugates and its transferase has been well analyzed. However, no β-D-galactofuranosidase (Galf-ase) gene has been identified in any organism. To search for a Galf-ase gene we screened soil samples and discovered a strain, identified as a Streptomyces species by the 16S ribosomal RNA gene analysis, that exhibits Galf-ase activity for 4-nitrophenyl β-D-galactofuranoside (pNP-β-D-Galf) in culture supernatants. By draft genome sequencing of the strain, named JHA19, we found four candidate genes encoding Galf-ases. Using recombinant proteins expressed in Escherichia coli, we found that three out of four candidates displayed the activity of not only Galf-ase but also α-L-arabinofuranosidase (Araf-ase), whereas the other one showed only the Galf-ase activity. This novel Galf-specific hydrolase is encoded by ORF1110 and has an optimum pH of 5.5 and a Km of 4.4 mM for the substrate pNP-β-D-Galf. In addition, this enzyme was able to release galactose residue from galactomannan prepared from the filamentous fungus Aspergillus fumigatus, suggesting that natural polysaccharides could be also substrates. By the BLAST search using the amino acid sequence of ORF1110 Galf-ase, we found that there are homolog genes in both prokaryotes and eukaryotes, indicating that Galf-specific Galf-ases widely exist in microorganisms. Copyright:

    DOI: 10.1371/journal.pone.0137230

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  • Identification and characterization of a novel galactofuranose-specific β-D-galactofuranosidase from Streptomyces species. Reviewed International journal

    2015.9

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  • Enzymatic characterization of a class II lysyl-rRNA synthetase, LysS, from Myxococcus xanthus. Reviewed International journal

    Oka M, Takegawa K, Kimura Y

    Archives of Biochemistry and Biophysics   2015.7

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  • Functional expression and characterization of schizosaccharomyces pombe Avt3p as a vacuolar amino acid exporter in saccharomyces cerevisiae Reviewed

    Chardwiriyapreecha S., Manabe K., Iwaki T., Kawano-Kawada M., Sekito T., Lunprom S., Akiyama K., Takegawa K., Kakinuma Y.

    PLoS ONE   10 ( 6 )   2015.6

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    In Saccharomyces cerevisiae, Avt3p and Avt4p mediate the extrusion of several amino acids from the vacuolar lumen into the cytosol. SpAvt3p of Schizosaccharomyces pombe, a homologue of these vacuolar amino acid transporters, has been indicated to be involved in spore formation. In this study, we confirmed that GFP-SpAvt3p localized to the vacuolar membrane in S. pombe. The amounts of various amino acids increased significantly in the vacuolar pool of avt3Δ cells, but decreased in that of avt3<sup>+</sup>-overexpressing avt3Δ cells. These results suggest that SpAvt3p participates in the vacuolar compartmentalization of amino acids in S. pombe. To examine the export activity of SpAvt3p, we expressed the avt3<sup>+</sup> gene in S. cerevisiae cells. We found that the heterologously overproduced GFP-SpAvt3p localized to the vacuolar membrane in S. cerevisiae. Using the vacuolar membrane vesicles isolated from avt3<sup>+</sup>-overexpressing S. cerevisiae cells, we detected the export activities of alanine and tyrosine in an ATP-dependent manner. These activities were inhibited by the addition of a V-ATPase inhibitor, concanamycin A, thereby suggesting that the activity of SpAvt3p is dependent on a proton electrochemical gradient generated by the action of V-ATPase. In addition, the amounts of various amino acids in the vacuolar pools of S. cerevisiae cells were decreased by the overproduction of SpAvt3p, which indicated that SpAvt3p was functional in S. cerevisiae cells. Thus, SpAvt3p is a vacuolar transporter that is involved in the export of amino acids from S. pombe vacuoles. Copyright:

    DOI: 10.1371/journal.pone.0130542

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  • Enzymatic characterization of a class II lysyl-tRNA synthetase, LysS, from Myxococcus xanthus Reviewed

    Oka M., Takegawa K., Kimura Y.

    Archives of Biochemistry and Biophysics   579   33 - 39   2015.6   ISSN:00039861

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    Lysyl-tRNA synthetases efficiently produce diadenosine tetraphosphate (Ap<inf>4</inf>A) from lysyl-AMP with ATP in the absence of tRNA. We characterized recombinant class II lysyl-tRNA synthetase (LysS) from Myxococcus xanthus and found that it is monomeric and requires Mn<sup>2+</sup> for the synthesis of Ap<inf>4</inf>A. Surprisingly, Zn<sup>2+</sup> inhibited enzyme activity in the presence of Mn<sup>2+</sup>. When incubated with ATP, Mn<sup>2+</sup>, lysine, and inorganic pyrophosphatase, LysS first produced Ap<inf>4</inf>A and ADP, then converted Ap<inf>4</inf>A to diadenosine triphosphate (Ap<inf>3</inf>A), and finally converted Ap<inf>3</inf>A to ADP, the end product of the reaction. Recombinant LysS retained Ap<inf>4</inf>A synthase activity without lysine addition. Additionally, when incubated with Ap<inf>4</inf>A (minus pyrophosphatase), LysS converted Ap<inf>4</inf>A mainly ATP and AMP, or ADP in the presence or absence of lysine, respectively. These results demonstrate that M. xanthus LysS has different enzymatic properties from class II lysyl-tRNA synthetases previously reported.

    DOI: 10.1016/j.abb.2015.05.014

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  • Functional analysis of conserved motifs in a bacterial tyrosine kinase, BtkB, from Mycococcus xanthus. Reviewed

    Kato T, Shirakawa Y, Takegawa K, Kimura Y

    Journal of Biochemistry   2015.6

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  • Functional expression and characterization of Schizosaccharomyces pombe Avt3p as a vacuolar amino acid exporter in Saccharomyces cerevisiae. Reviewed International journal

    Chardwiriyapreecha S, Manabe S, Iwaki T, Kawano-kawada M, Sekito T, Lnprom S, Akiyama K, Takegawa K, Kakinuma Y

    PLOS ONE   2015.6

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  • Coordinated regulation by two VPS9 domain-containing guanine nucleotide exchange factors in small GTPase Rab5 signaling pathways in fission yeast Reviewed

    Tsukamoto Y., Kagiwada S., Shimazu S., Takegawa K., Noguchi T., Miyamoto M.

    Biochemical and Biophysical Research Communications   458 ( 4 )   802 - 809   2015.3   ISSN:0006291X

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    Abstract The small GTPase Rab5 is reported to regulate various cellular functions, such as vesicular transport and endocytosis. VPS9 domain-containing proteins are thought to activate Rab5(s) by their guanine-nucleotide exchange activities. Numerous VPS9 proteins have been identified and are structurally conserved from yeast to mammalian cells. However, the functional relationships among VPS9 proteins in cells remain unclear. Only one Rab5 and two VPS9 proteins were identified in the Schizosaccharomyces pombe genome. Here, we examined the cellular function of two VPS9 proteins and the relationship between these proteins in cellular functions. Vps901-GFP and Vps902-GFP exhibited dotted signals in vegetative and differentiated cells. vps901 deletion mutant (Δvps901) cells exhibited a phenotype deficient in the mating process and responses to high concentrations of ions, such as calcium and metals, and Δvps901Δvps902 double mutant cells exhibited round cell shapes similar to ypt5-909 (Rab5 mutant allele) cells. Deletion of both vps901 and vps902 genes completely abolished the mating process and responses to various stresses. A lack of vacuole formation and aberrant inner cell membrane structures were also observed in Δvps901Δvps902 cells by electron microscopy. These data strongly suggest that Vps901 and Vps902 are cooperatively involved in the regulation of cellular functions, such as cell morphology, sexual development, response to ion stresses, and vacuole formation, via Rab5 signaling pathways in fission yeast cells.

    DOI: 10.1016/j.bbrc.2015.02.031

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  • Functional analysis of conserved motifs in a bacterial tyrosine kinase, BtkB, from Myxococcus xanthus Reviewed

    Kato T., Shirakawa Y., Takegawa K., Kimura Y.

    Journal of Biochemistry   158 ( 5 )   385 - 392   2015.3   ISSN:0021924X

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    Myxococcus xanthus has two bacterial protein-tyrosine (BY) kinases, BtkA and BtkB. Autophosphorylation in C-terminal tyrosine-rich clusters and poly(Glu, Tyr) kinase activities of cytoplasmic catalytic domains of BtkA and BtkB were activated by the intracellular juxtamembrane regions of the second transmembrane helices. Protein kinase activity against poly(Glu, Tyr) of cytoplasmic fragment of BtkB (CF-BtkB) containing an activator region was not inhibited by serine/threonine protein kinase inhibitors. However, addition of tyrosine protein kinase inhibitors, genistein and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), at a concentration of 0.2 mM, inhibited the CF-BtkB kinase activity by 20 and 64%, respectively. A CF-BtkB mutant constructed by replacing all C-terminal tyrosine residues with phenylalanines, did not undergo autophosphorylation. Further, this mutation did not significantly affect poly(Glu, Tyr) kinase activity, suggesting that M. xanthus BtkB kinase activity is not dependent on autophosphorylation in the C-terminal tyrosine cluster. A conserved motif (ExxRxxR) of BY kinases is involved in the self-association of catalytic domains of BY kinases, necessary to accomplish trans-phosphorylation. An ExxRxxR motif mutant of CF-BtkB led to loss of autophosphorylation and poly(Glu, Tyr) kinase activities. These observations provide insights into the regulation mechanism of M. xanthus BY kinase activity.

    DOI: 10.1093/jb/mvv053

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  • Coordinated regulations by two VPS9 domain-containing guanine-nucleotide exchange factors. Reviewed International journal

    Tsukamoto Y, Kagiwada S, Shimazu S, Takegawa K, Noguchi T, Miyamoto M

    Biochem. Biophys. Res. Commun.   2015.3

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  • Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe. Reviewed International journal

    Microbiology   2015.1

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  • Draft genome sequence of Streptomyces sp. JHA19, a strain that possesses β-Dgalactofuranosidase activity Reviewed

    Matsunaga E., Higuchi Y., Mori K., Tashiro K., Kuhara S., Takegawa K.

    Genome Announcements   3 ( 5 )   2015

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    By screening for microbes that exhibit β-D-galactofuranosidase (Galf-ase) activity, a Streptomyces sp. strain, named JHA19, was isolated from a soil sample from Kagawa University, Japan, in 2010. Here, we report the results of whole-genome shotgun sequencing and found that the strain has four predicted Galf-ase genes.

    DOI: 10.1128/genomeA.01171-15

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  • Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe Reviewed International journal

    Yoritsune KI, Higuchi Y, Matsuzawa T, Takegawa K

    FEMS Yeast Research   2014.12

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  • Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe Reviewed

    Yoritsune K.I., Higuchi Y., Matsuzawa T., Takegawa K.

    FEMS Yeast Research   14 ( 7 )   1101 - 1109   2014.11   ISSN:15671356

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    The cell surface of Schizosaccharomyces pombe is negatively charged due to the presence of pyruvylated oligosaccharides, which is important for cell-cell recognition. However, the mechanism of pyruvate supply to oligosaccharides is not clearly understood. Here, we analyzed three putative phosphoenolpyruvate (PEP) transporter genes (pet1+, pet2+, and pet3+) in S. pombe, identified by sequence homology search against the Arabidopsis thaliana PEP transporter AtPPT1. Schizosaccharomyces pombe strain carrying a disruption in pet1+ (pet1Δ) or in pet2+ (pet2Δ), but not the strain carrying a disruption in pet3+ (pet3Δ), showed reduced pyruvate level on the cell surface. This reduction in pyruvate level was restored to the control level by expressing green fluorescent protein (GFP)-tagged Pet1p and Pet2p in respective disruptants. Fluorescence microscope studies revealed that GFP-tagged Pet1p and Pet2p were localized to the Golgi apparatus. Although expression of neither AtPPT1 nor AtPPT2 suppressed the pet1Δ phenotype, that of chimeric constructs, where the N-terminal regions of AtPPT1 and AtPPT2 were replaced by the N-terminal region of Pet1p, partially suppressed the pet1Δ phenotype. Furthermore, the reduction in cell surface negative charge in pet1Δ cells was restored by incubating these cells with recombinant Pvg1p and PEP. Thus, Pet1p and Pet2p are likely involved in transporting PEP from the cytoplasm into the Golgi.

    DOI: 10.1111/1567-1364.12207

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  • Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus Reviewed

    Sasaki M., Takegawa K., Kimura Y.

    FEBS Letters   588 ( 18 )   3395 - 3402   2014.9   ISSN:00145793

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    We characterized the activities of the Myxococcus xanthus ApaH-like phosphatases PrpA and ApaH, which share homologies with both phosphoprotein phosphatases and diadenosine tetraphosphate (Ap<inf>4</inf>A) hydrolases. PrpA exhibited a phosphatase activity towards p-nitrophenyl phosphate (pNPP), tyrosine phosphopeptide and tyrosine-phosphorylated protein, and a weak hydrolase activity towards Ap<inf>n</inf>A and ATP. In the presence of Mn<sup>2+</sup>, PrpA hydrolyzed Ap<inf>4</inf>A into AMP and ATP, whereas in the presence of Co<sup>2+</sup> PrpA hydrolyzed Ap<inf>4</inf>A into two molecules of ADP. ApaH exhibited high phosphatase activity towards pNPP, and hydrolase activity towards Ap<inf>n</inf>A and ATP. Mn<sup>2+</sup> was required for ApaH-mediated pNPP dephosphorylation and ATP hydrolysis, whereas Co<sup>2+</sup> was required for Ap<inf>n</inf>A hydrolysis. Thus, PrpA and ApaH may function mainly as a tyrosine protein phosphatase and an Ap<inf>n</inf>A hydrolase, respectively.

    DOI: 10.1016/j.febslet.2014.07.031

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  • Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus. Reviewed International journal

    Sasaki M, Takegawa K, Kimura Y

    FEBS Letters   2014.9

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  • Regulation of eukaryotic-like protein kinase activity of DspA from Myxococcus xanthus by autophosphorylation Reviewed

    Okamoto R., Takegawa K., Kimura Y.

    Journal of Biochemistry   155 ( 2 )   99 - 106   2014.2   ISSN:0021924X

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    A Myxococcus xanthus DspA contains 12 subdomains characteristic of eukaryotic-like protein kinases but with an atypical sequence, RDxSPHN, in the catalytic loop, different from the consensus motifs observed in Ser/Thr kinases (RDxKxxN) or Tyr kinases (RDx(A/R)A(A/R)N). DspA phosphorylated myelin basic protein (MBP) on Ser and Thr residues. Mutations of the SPHN motif within the catalytic loop to KPHN or KPEN for Ser/Thr kinases, AARN for Tyr kinases and TPHN or TSHN for Dictyostelium Tyr kinases markedly reduced autophosphorylation and kinase activities. Phosphorylation assays, Western blot analysis and mutational analysis revealed that DspA is a dual-specificity kinase that autophosphorylates on two Thr residues (Thr-199 and Thr-201) in the activation loop and two Tyr residues (Tyr-35 and Tyr-111). RD kinases such as DspA are activated by phosphorylation in the activation loop. Replacement of Thr-199 or/and Thr-201 in the DspA activation loop by alanine also almost abolished autophosphorylation and kinase activities. In addition, mutation of either Tyr-35 or Tyr-111 to phenylalanine decreased kinase activities against MBP, and double mutation abolished kinase activity. These results suggested that DspA is activated by dual autophosphorylation of Thr residues in the activation loop, and autophosphorylation on two Tyr residues of DspA are required for high-level kinase activity. © 2013 The Authors.

    DOI: 10.1093/jb/mvt101

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  • Regulation of eukaryotic-like protein kinase activity of DspA from Myxococcus xanthus by autophosphorylation. Reviewed International journal

    Okamoto R, Takegawa K, Kimura Y

    Journal of Biochemistry   2014.2

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  • The purative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans. Reviewed

    Futagami T, Seto K, Kajiwara Y, Takashita H, Omori T, Takegawa K, Goto M

    Bioscience, Biotechnology, and Biochemistry   2014.1

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  • gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus Reviewed

    Komachi Y., Hatakeyama S., Motomatsu H., Futagami T., Kizjakina K., Sobrado P., Ekino K., Takegawa K., Goto M., Nomura Y., Oka T.

    Molecular Microbiology   90 ( 5 )   1054 - 1073   2013.12   ISSN:0950382X

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    Summary: The cells walls of filamentous fungi in the genus Aspergillus have galactofuranose (Galf)-containing polysaccharides and glycoconjugates, including O-glycans, N-glycans, fungal-type galactomannan and glycosylinositolphosphoceramide, which are important for cell wall integrity. Here, we attempted to identify galactofuranosyltransferases that couple Galf monomers onto other wall components in Aspergillus nidulans. Using reverse-genetic and biochemical approaches, we identified that the AN8677 gene encoded a galactofuranosyltransferase, which we called GfsA, involved in Galf antigen biosynthesis. Disruption of gfsA reduced binding of β-Galf-specific antibody EB-A2 to O-glycosylated WscA protein and galactomannoproteins. The results of an in-vitroGalf antigen synthase assay revealed that GfsA has β1,5- or β1,6-galactofuranosyltransferase activity for O-glycans in glycoproteins, uses UDP-d-Galf as a sugar donor, and requires a divalent manganese cation for activity. GfsA was found to be localized at the Golgi apparatus based on cellular fractionation experiments. ΔgfsA cells exhibited an abnormal morphology characterized by poor hyphal extension, hyphal curvature and limited formation of conidia. Several gfsA orthologues were identified in members of the Pezizomycotina subphylum of Ascomycota, including the human pathogen Aspergillus fumigatus. To our knowledge, this is the first characterization of a fungal β-galactofuranosyltransferase, which was shown to be involved in Galf antigen biosynthesis of O-glycans in the Golgi. © 2013 John Wiley & Sons Ltd.

    DOI: 10.1111/mmi.12416

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  • Functional expression of schizosaccharomyces pombe Vba2p in the vacuolar membrane of Saccharomyces cerevisiae Reviewed

    Pongcharoen P., Kawano-Kawada M., Iwaki T., Sugimoto N., Sekito T., Akiyama K., Takegawa K., Kakinuma Y.

    Bioscience, Biotechnology and Biochemistry   77 ( 9 )   1988 - 1990   2013.10   ISSN:09168451

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    A vacuolar membrane protein, Vba2p of Schizosaccharomyces pombe, is involved in basic amino acid uptake by intact cells. Here we found evidence that Vba2p mediated ATP-dependent lysine uptake by vacuolar membrane vesicles of Saccharomyces cerevisiae. Vba2p was also responsible for quinidine sensitivity, and the addition of lysine improved cell growth on quinidine-containing media. These findings should be useful for further characterization of Vba2p.

    DOI: 10.1271/bbb.130387

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  • Functional expression of Schizosaccharomyces pombe Vba2p in vacuolar membrane of Saccharomyces cerevisiae. Reviewed

    Pongharoen P, Kawano-Kawada M, Iwaki T, Sugimoto N, Sekito T, Akiyama K, Takegawa K, Kakinuma Y

    Bioscience, Biotechnology, and Biochemistry   2013.9

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  • Ethanol-inducible gene expression using gld1+ promoter in the fission yeast Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Tohda H, Takegawa K

    Applied Microbiology and Biotechnology   2013.8

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  • Ethanol-inducible gene expression using gld1 <sup>+</sup> promoter in the fission yeast Schizosaccharomyces pombe Reviewed

    Matsuzawa T., Tohda H., Takegawa K.

    Applied Microbiology and Biotechnology   97 ( 15 )   6835 - 6843   2013.8   ISSN:01757598

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    In the fission yeast Schizosaccharomyces pombe, the gld1 + gene encoding glycerol dehydrogenase is repressed by glucose and induced by ethanol and 1-propanol. The promoter region of gld1 + was cloned into a multicopy vector designated as pEG1 for evaluation as an ethanol-inducible expression vector using EGFP as a model heterologous protein. Expression of EGFP was repressed in the presence of high glucose and induced in the presence of ethanol, low-glucose, and 1-propanol in the absence of glucose. Addition of ethanol to cells harboring pEG1-EGFP was found to be the most effective means for inducing EGFP production. Protein yields were found to increase in proportion to ethanol concentration. As a further test of effectiveness, secreted recombinant human growth hormone was produced using the pEG1 expression vector in medium containing glycerol and ethanol. The pEG1 gene expression system is an effective tool for the production of heterologous proteins under glucose-limiting conditions, including medium containing glycerol as a carbon source. © 2013 Springer-Verlag Berlin Heidelberg.

    DOI: 10.1007/s00253-013-4812-2

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  • Construction of a ligD disruptant for efficient gene targeting in white koji mold, Aspergillus kawachii. Reviewed

    Tashiro S, Futagami T, Wada S, Kajiwara Y, Takashita H, Omori T, Takahashi T, Yamada O, Takegawa K, Goto M

    Journal of General and Applied Microbiology   2013.7

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  • Ght2<sup>+</sup> is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe Reviewed

    Matsuzawa T., Hara F., Tanaka N., Tohda H., Takegawa K.

    Applied Microbiology and Biotechnology   97 ( 11 )   4957 - 4964   2013.6   ISSN:01757598

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    Schizosaccharomyces pombe has eight hexose transporter genes, ght1 + to ght8 +. Here we report that ght2 +, which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 + in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose. © 2012 Springer-Verlag Berlin Heidelberg.

    DOI: 10.1007/s00253-012-4637-4

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  • ght2+ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Hara F, Tanaka N, Tohda H, Takegawa K

    Applied Microbiology and Biotechnology   2013.5

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  • Evaluation of the inhibitory effects of chloroform on ortho-chlorophenol- and chloroethene-dechlorinating Desulfitobacterium strains. Reviewed International journal

    Futagami T, Fukaki Y, Fujihara H, Takegawa K, Goto M, Furukawa K

    AMB Express   2013.5

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  • Characterization of genome-reduced fission yeast strains Reviewed

    Sasaki M., Kumagai H., Takegawa K., Tohda H.

    Nucleic Acids Research   41 ( 10 )   5382 - 5399   2013.5   ISSN:03051048

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    The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the left arm of chromosome I, 155.4 kb in the right arm of chromosome I, 211.7 kb in the left arm of chromosome II and 121.6 kb in the right arm of chromosome II. The deletions corresponded to a loss of 223 genes of the original ∼5100. The quadruple-deletion strain, with a total deletion size of 657.3 kb, showed a decreased ability to uptake glucose and some amino acids in comparison with the parental strain. The strain also showed increased gene expression of the mating pheromone M-factor precursor and the nicotinamide adenine dinucleotide phosphate -specific glutamate dehydrogenase. There was also a 2.7-fold increase in the concentration of cellular adenosine triphosphate, and levels of the heterologous proteins, enhanced green fluorescent protein and secreted human growth hormone were increased by 1.7- and 1.8-fold, respectively. The transcriptome data from this study have been submitted to the Gene Expression Omnibus (GEO: http://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE38620 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token= vjkxjewuywgcovc&acc=GSE38620). © 2013 The Author(s) 2013.

    DOI: 10.1093/nar/gkt233

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  • The fission yeast Pvg1p shows galactose-specific pyruvyltransferase activity. Reviewed International journal

    Yoritsune Ken-ichi, Matsuzawa Tomohiko, Ohashi Takao, Takegawa Kaoru

    FEBS Letters   587 ( 7 )   917 - 921   2013.3

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  • The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast. Reviewed International journal

    Matsuzawa T, Kageyama Y, Ooishi K, Kawamukai M, Takegawa K

    FEMS Yeast Research   13(3)   259 - 266   2013.3

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  • Evaluation of the inhibitory effects of chloroform on ortho-chlorophenol- and chloroethenedechlorinating Desulfitobacterium strains Reviewed

    Futagami T., Fukaki Y., Fujihara H., Takegawa K., Goto M., Furukawa K.

    AMB Express   3   1 - 8   2013

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    Organohalide-respiring Desulfitobacterium strains are believed to play an important role in the bioremediation and natural attenuation of chlorinated aliphatic and aromatic hydrocarbons. However, several studies have reported that chloroform significantly inhibits microbial reductive dechlorination of chloroethene. In this study, we examined the effect of chloroform on several Desulfitobacterium strains, including ortho-chlorophenol-dechlorinating Desulfitobacterium dehalogenans JW/IU-1 and Desulfitobacterium hafniense DCB-2, and also the chloroethene-dechlorinating strain D. hafniense TCE1. In medium containing 3-chloro-4-hydroxyphenylacetate as an electron acceptor, chloroform inhibited the growth of strains JW/IU-1 and DCB-2. Although chloroform did not directly inhibit dechlorination of 3-chloro-4-hydroxyphenylacetate by resting cells, cells cultivated with chloroform showed decreased dechlorination activity. Moreover, transcription of the gene encoding the reductive dehalogenase CprA decreased significantly in cells cultivated with chloroform. These results indicate that chloroform inhibits the growth and dechlorination activity of strains JW/IU-1 and DCB-2 via inhibition of cprA transcription. In contrast, cultivation of strain TCE1 in the presence of chloroform gave rise to a PceA reductive dehalogenase gene-deletion variant of strain TCE1; a similar phenomenon was observed in our previous study of chloroethene-dechlorinating D. hafniense strain Y51. Our results suggest that chloroform extensively inhibits the dechlorination activity of Desulfitobacterium strains, and that the inhibitory mechanism appears to differ between ortho-chlorophenol dechlorinators and chloroethene dechlorinators. © 2013 Bel-Rhlid et al.

    DOI: 10.1186/2191-0855-3-30

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  • Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe Reviewed

    Ohashi T., Fujiyama K., Takegawa K.

    Journal of Biological Chemistry   287 ( 46 )   38866 - 38875   2012.11   ISSN:00219258

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    The oligosaccharides from fission yeast Schizosaccharomyces pombe contain large amounts of D-galactose (Gal) in addition to D-mannose (Man), in contrast to the budding yeast Saccharomyces cerevisiae. Detailed structural analysis has revealed that the Gal residues are attached to the N- and O-linked oligosaccharides via α1,2- or α1,3-linkages. Previously we constructed and characterized a septuple α-galactosyltransferase disruptant (7GalTΔ) anticipating a complete lack of α-Gal residues. However, the 7GalTΔ strain still contained oligosaccharides consisting of α1,3-linked Gal residues, indicating the presence of at least one more additional unidentified α1,3-galactosyltransferase. In this study we searched for unidentified putative glycosyltransferases in the S. pombe genome sequence and identified three novel genes, named otg1+-otg3 + (α one, three-galactosyltransferase), that belong to glycosyltransferase gene family 8 in the Carbohydrate Active EnZymes (CAZY) database. Gal-recognizing lectin blotting and HPLC analyses of pyridylaminated oligosaccharides after deletion of these three additional genes from 7GalTΔ strain demonstrated that the resultant disruptant missing 10 α-galactosyltransferase genes, 10GalTΔ, exhibited a complete loss of galactosylation. In an in vitro galactosylation assay, the otg2+ gene product had Gal transfer activity toward a pyridylaminated Man 9GlcNAc2 oligosaccharide and pyridylaminated Manα1,2-Manα1,2-Man oligosaccharide. In addition, the otg3 + gene product exhibited Gal transfer activity toward the pyridylaminated Man9GlcNAc2 oligosaccharide. Generation of an α1,3-linkage was confirmed by HPLC analysis, α-galactosidase digestion analysis, 1H NMR spectroscopy, and LC-MS/MS analysis. These results indicate that Otg2p and Otg3p are involved in α1,3- galactosylation of S. pombe oligosaccharides. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

    DOI: 10.1074/jbc.M112.347351

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  • A tyrosine phosphatase, PhpA, of Myxococcus xanthus is involved in the production of exopolysaccharide. Reviewed International journal

    Mori Y, Maeda M, Takegawa K, and Kimura Y

    Microbiology   2012.11

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  • PhpA, a tyrosine phosphatase of Myxococcus xanthus, is involved in the production of exopolysaccharide Reviewed

    Mori Y., Maeda M., Takegawa K., Kimura Y.

    Microbiology (United Kingdom)   158 ( 10 )   2546 - 2555   2012.10   ISSN:13500872

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    Protein-tyrosine phosphorylation plays a significant role in multiple cellular functions in bacteria. Bacterial tyrosine phosphatases catalyse the dephosphorylation of tyrosyl-phosphorylated proteins. Myxococcus xanthus PhpA shares homology with DNA polymerase and histidinol phosphatase family members. Recombinant His-tagged PhpA requires Mn2+ or Co2+ for phosphatase activity, and shows strict specificity for phosphorylated tyrosine residues. The km values of PhpA for p-nitrophenyl phosphate (pNPP) and phosphotyrosine peptide, RRLIEDAEpYAARG, were 803 and 139 μM, respectively. The phosphatase activity of PhpA was inhibited by sodium orthovanadate with a ki of 33 μM. phpA gene expression was observed under both vegetative and developmental conditions, but peaked during late fruiting body formation. A phpA mutant exhibited an elevated level of tyrosine phosphorylation of a 79 kDa protein and cytoplasmic tyrosine kinase, BtkA. In M. xanthus, exopolysaccharide (EPS) is essential for cell-cell adhesion and fruiting body formation. phpA mutant cells exhibited enhanced capacity for cell- cell agglutination in agglutination buffer. Under starvation conditions, phpA mutation caused early aggregation and sporulation. The EPS production assay showed that the phpA mutant produced an increased amount of EPS in comparison with the wild-type. These results indicate that PhpA may negatively regulate the production of EPS in M. xanthus. © 2012 SGM.

    DOI: 10.1099/mic.0.059824-0

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  • Function analysis of conserved amino acid residues in a Mn2+-dependent protein phosphatase, Pph3, from Myxococcus xanthus. Reviewed International journal

    Mori Y, Takegawa K, and Kimura Y

    Journal of Biochemistry   2012.9

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  • Function analysis of conserved amino acid residues in a Mn <sup>2+</sup>-dependent protein phosphatase, Pph3, from Myxococcus xanthus Reviewed

    Mori Y., Takegawa K., Kimura Y.

    Journal of Biochemistry   152 ( 3 )   269 - 274   2012.9   ISSN:0021924X

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    The Myxococcus xanthus protein phosphatase Pph3 belongs to the Mg 2+-or Mn2+-dependent protein phosphatase (PPM) family. Bacterial PPMs contain three divalent metal ions and a flap subdomain. Putative metal-or phosphate-ion binding site-specific mutations drastically reduced enzymatic activity. Pph3 contains a cyclic nucleotide monophosphate (cNMP)-binding domain in the C-terminal region, and it requires 2-mercaptoethanol for phosphatase activity; however, the C-terminal deletion mutant showed high activity in the absence of 2-mercaptoethanol. The phosphatase activity of the wild-type enzyme was higher in the presence of cAMP than in the absence of cAMP, whereas a triple mutant of the cNMP-binding domain showed slightly lower activities than those of wild-type, without addition of cAMP. In addition, mutational disruption of a disulphide bond in the wild-type enzyme increased the phosphatase activity in the absence of 2-mercaptoethanol, but not in the C-terminal deletion mutant. These results suggested that the presence of the C-terminal region may lead to the formation of the disulphide bond in the catalytic domain, and that disulphide bond cleavage of Pph3 by 2-mercaptoethanol may occur more easily with cAMP bound than with no cAMP bound. © 2012 The Authors.

    DOI: 10.1093/jb/mvs067

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  • Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Hara F, Tohda H, Uemura H, and Takegawa K

    Applied Microbiology and Biotechnology   2012.7

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  • Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe Reviewed

    Matsuzawa T., Hara F., Tohda H., Uemura H., Takegawa K.

    Applied Microbiology and Biotechnology   95 ( 2 )   441 - 449   2012.7   ISSN:01757598

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    The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1 + and fbp1 + during glucose starvation even though gld1 + and fbp1 + are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1 + was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1 + and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation. © Springer-Verlag 2012.

    DOI: 10.1007/s00253-012-3971-x

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  • Expression of budding yeast IPT1 gene produces mannosyldiinositol phosphorylceramide in fission yeast and inhibits cell growth. Reviewed International journal

    Nakase M, Tani M, and Takegawa K

    Microbiology   2012.5

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  • Expression of budding yeast IPT1 produces mannosyldiinositol phosphorylceramide in fission yeast and inhibits cell growth Reviewed

    Nakase M., Tani M., Takegawa K.

    Microbiology   158 ( 5 )   1219 - 1228   2012.5   ISSN:13500872

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    In Saccharomyces (Sacc.) cerevisiae, the final step of the complex sphingolipid biosynthetic pathway requires Ipt1p for synthesis of mannosyldiinositol phosphorylceramide [M(IP)2C]. No fission yeast equivalent to Ipt1p has been found in the Schizosaccharomyces (Schiz.) pombe genome, and the most abundant complex sphingolipid is mannosylinositol phosphorylceramide. To examine the effect of expressing Sacc. cerevisiae IPT1 (ScIPT1) in Schiz. pombe, the ScIPT1 gene was cloned into an inducible fission yeast integrative vector and expressed in wild-type Schiz. pombe. In the Schiz. pombe ScIPT1-expressing cells, M(IP)2C was detected, indicating that ScIpt1p functions in M(IP)2C synthesis in Schiz. pombe. Expression of ScIPT1 caused pleiotropic phenotypes, including aberrant morphology and mislocalization of ergosterols in the plasma membrane. Furthermore, growth of Schiz. pombe was severely impaired. We analysed the sphingolipid composition of ScIPT1-expressing cells following a prolonged lag phase, and found that M(IP)2C was not synthesized, indicating that Ipt1p had been inactivated. GFP-tagged ScIpt1 localized primarily in the Golgi apparatus in wild-type Schiz. pombe. Over time, ScIpt1p was eventually transported to the vacuolar lumen through the multivesicular body pathway. These results indicate that M(IP)2C is toxic to Schiz. pombe and that fission yeast possesses an unknown mechanism to effectively extrude toxic sphingolipids from cells. © 2012 SGM.

    DOI: 10.1099/mic.0.056184-0

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  • CUE domain-containing protein vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe Reviewed

    Nakase M., Tsukamoto Y., Hosomi A., Matsuda T., Miyamoto M., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   76 ( 4 )   652 - 659   2012.4   ISSN:09168451

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    The functions of two Schizosaccharomyces pombe Vps9- like genes, SPBC4F6.10/vps901+ and SPBC29A10.11c/ vps902+, were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of vps901+ resulted in mis-sorting and secretion of S. pombe vacuolar carboxypeptidase Cpy1p, whereas deletion of vps902+ had no effect, suggesting that only Vps901p functions in vacuolar protein transport in S. pombe. Deletion of vps901+ further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast VPS9 gene corrected the CPY mis-sorting defect in vps901Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in S. pombe.

    DOI: 10.1271/bbb.110609

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  • Guidelines for the use and interpretation of assays for monitoring autophagy Reviewed

    Klionsky D.J., Abdalla F.C., Abeliovich H., Abraham R.T., Acevedo-Arozena A., Adeli K., Agholme L., Agnello M., Agostinis P., Aguirre-Ghiso J.A., Ahn H.J.u., Ait-Mohamed O., Ait-Si-Ali S., Akematsu T., Akira S., Al-Younes H.M., Al-Zeer M.A., Albert M.L., Albin R.L., Alegre-Abarrategui J., Aleo M.F.r., Alirezaei M., Almasan A., Almonte-Becerril M., Amano A., Amaravadi R., Amarnath S., Amer A.O., Andrieu-Abadie N., Anantharam V., Ann D.K., Anoopkumar-Dukie S., Aoki H., Apostolova N., Arancia G., Aris J.P., Asanuma K., Asare N.Y.O., Ashida H., Askanas V., Askew D.S., Auberger P., Baba M., Backues S.K., Baehrecke E.H., Bahr B.A., Bai X.Y., Bailly Y., Baiocchi R., Baldini G., Balduini W., Ballabio A., Bamber B.A., Bampton E.T.W., Bánhegyi G., Bartholomew C.R., Bassham D.C., Bast R.C., Batoko H., Bay B.H., Beau I., Béchet D.M., Begley T.J., Behl C., Behrends C., Bekri S., Bellaire B., Bendall L.J., Benetti L., Berliocchi L., Bernardi H., Bernassola F., Besteiro S., Bhatia-Kissova I., Bi X., Biard-Piechaczyk M., Blum J.S., Boise L.H., Bonaldo P., Boone D.L., Bornhauser B.C., Bortoluci K.R., Bossis I., Bost F., Bourquin J.P., Boya P., Boyer-Guittaut M., Bozhkov P.V., Brady N.R., Brancolini C., Brech A., Brenman J.E., Brennand A., Bresnick E.H., Brest P., Bridges D., Bristol M.L., Brookes P.S., Brown E.J., Brumell J.H.

    Autophagy   8 ( 4 )   445 - 544   2012.4

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    In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.

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  • CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe. Reviewed

    Nakase M, Tsukamoto Y, Hosomi A, Tsuji H, Matsuda T, Miyamoto M, and Takgawa K

    Bioscience, Biotechnology, and Biochemistry   2012.4

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  • Guidelines for the use and interpretation of assays for monitoring autophagy. Invited International journal

    Klionsky DJ and 1268 authors

    Autophagy   2012.4

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  • Genome sequence of the Pantoea agglomerans strain IG1. Reviewed International journal

    Matsuzawa T, Mori K, Kadowaki T, Shimada M, Tashiro K, Kuhara S, Inagawa H, Soma G, and Takegawa K

    Journal of Bacteriology   2012.3

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  • Galactose-specific recognition system in the fission yeast Schizosaccharomyces pombe. Reviewed

    Matsuzawa T, Ohashi T, Nakase M, Yoritsune K, and Takegawa K

    Trends in Glycoscience and Glycotechnology   2012.3

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  • The ubiquitin ligase Ubr11 is essential for the oligopeptide utilization in fission yeast Schizosaccharomyces pombe. Reviewed International journal

    Kitamura K, Nakase M, Tohda H, and Takegawa K

    Eukaryotic Cell   2012.3

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  • Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid pearmease Aat1p. Reviewed International journal

    Nakase M, Nakase Y, Chardwiriyapreecha S, Kakinuma Y, Matsumoto T, and Takegawa K

    Microbiology   2012.3

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  • Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p Reviewed

    Nakase M., Nakase Y., Chardwiriyapreecha S., Kakinuma Y., Matsumoto T., Takegawa K.

    Microbiology   158 ( 3 )   659 - 673   2012.3   ISSN:13500872

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    In Schizosaccharomyces pombe, neither intracellular sorting nor ubiquitination of amino acid permeases is well understood. In the present study, we show that intracellular sorting of the amino acid permease Aat1p in S. pombe depends on the presence of a nitrogen source in the growth medium. Under nitrogen-sufficient conditions, Aat1p appeared to be stably localized at the Golgi apparatus. In contrast, under nitrogen-insufficient conditions, Aat1p was sorted to the plasma membrane. Over time, plasma membrane-localized Aat1p was internalized and sorted to the lumen of the vacuole, where it was degraded. Sorting of Aat1p to the vacuolar lumen was dependent on the ESCRT (endosomal sorting complex required for transport) complex, which is required for formation of the multivesicular body. S. pombe has three genes (pub1 +, pub2 + and pub3 +) that are homologous to the ubiquitin ligase RSP5. Under nitrogen-sufficient conditions, Aat1-GFP was missorted to the plasma membrane in pub1D cells and ubiquitinated Aat1p was not detected. These results suggest that Pub1p-mediated ubiquitination is required for retention of Aat1 at the Golgi under nitrogen-sufficient conditions. The Aat1p lysine mutant Aat1 K18, 26, 27 was completely missorted to the plasma membrane under nitrogen-rich conditions. Furthermore, Aat1 K4, 18R, Aat1 K4, 26, 27R and Aat1 K18, 26, 27K mutants were severely blocked in endocytosis. These results indicate that ubiquitination is an important determinant for localization and regulation of the Aat1p permease in S. pombe. © 2012 SGM.

    DOI: 10.1099/mic.0.053389-0

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  • Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Fujita Y, Tohda H, and Takegawa K

    Eukaryotic Cell   2012.2

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  • N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe. Reviewed International journal

    Mukaiyama H, Kodera M, Tanaka N, Takegawa, K.

    Applied Microbiology and Biotechnology   2012.2

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  • MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast. Reviewed International journal

    Matsuzawa T, Yoritsune K, and Takegawa K

    Eukaryotic Cell   2012.2

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  • N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe Reviewed

    Mukaiyama H., Kodera M., Tanaka N., Takegawa K.

    Applied Microbiology and Biotechnology   93 ( 4 )   1609 - 1618   2012.2   ISSN:01757598

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    In eukaryotic cells, aberrant proteins generated in the endoplasmic reticulum (ER) are degraded by the ER-associated degradation (ERAD) pathway. Here, we report on the ERAD pathway of the fission yeast Schizosaccharomyces pombe. We constructed and expressed Saccharomyces cerevisiae wild-type CPY (ScCPY) and CPY-G255R mutant (ScCPYY*) in S. pombe. While ScCPY was glycosylated and efficiently transported to the vacuoles in S. pombe, ScCPYY*was retained in the ER and was not processed to the matured form in these cells. Cycloheximide chase experiments revealed that ScCPYY*was rapidly degraded in S. pombe, and its degradation depended on Hrd1p and Ubc7p homologs. We also found that Mnl1p and Yos9p, proteins that are essential for ERAD in S. cerevisiae, were not required for ScCPY*degradation in S. pombe. Moreover, the null-glycosylation mutant of ScCPY, CPY*0000, was rapidly degraded by the ERAD pathway. These results suggested that N-linked oligosaccharides are not important for the recognition of luminal proteins for ERAD in S. pombe cells. © 2011 Springer-Verlag.

    DOI: 10.1007/s00253-011-3662-z

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  • Mads box transcription factor Mbx2/pvg4 regulates invasive growth and flocculation by inducing Gsf2 <sup>+</sup> expression in fission yeast Reviewed

    Matsuzawa T., Yoritsune K.i., Takegawa K.

    Eukaryotic Cell   11 ( 2 )   151 - 158   2012.2   ISSN:15359778

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    The fission yeast Schizosaccharomyces pombe exhibits invasive growth and nonsexual flocculation in response to nitrogen limitation. Gsf2, a flocculin of fission yeast, is required not only for nonsexual flocculation but also for invasive growth through the recognition of galactose residues on cell surface glycoconjugates. We found that pyruvylation negatively regulates nonsexual flocculation by capping the galactose residues of N-linked galactomannan. We investigated whether pyruvylation also regulates invasive growth. The pvg4 + gene originally was isolated as a multicopy suppressor of apvg4 mutant defective in the pyruvylation of N-linked oligosaccharides. However, we did not detect a defect in cell surface pyruvylation in the pvg4/mbx2 deletion mutant, as assessed by alcian blue staining and a Q-Sepharose binding assay. Instead, the deletion prevented invasive growth under conditions of low nitrogen and high glucose, and it reduced the adhesion and flocculation of otherwise flocculent mutants by reducing gsf2 + expression. mbx2 +-overexpressing strains exhibited nonsexual and calcium-dependent aggregation, which was inhibited in the presence of galactose but mediated by the induction ofgsf2 +. These findings indicate that Mbx2 mediates invasive growth and flocculation via the transcriptional activation ofgsf2 + in fission yeast. In addition, we found that fission yeast Mbx2 induces the nonsexual flocculation of budding yeast by the activation of FLO1. © 2012, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/EC.05276-11

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  • Galactose-Specific Recognition System in the Fission Yeast Schizosaccharomyces pombe Reviewed

    Matsuzawa T., Ohashi T., Nakase M., Yoritsune K.i., Takegawa K.

    Trends in Glycoscience and Glycotechnology   24 ( 135 )   24 - 42   2012   ISSN:09157352

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    In the fission yeast Schizosaccharomyces pombe, galactose residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases in the lumen of the Golgi apparatus. Although galactose residues are not essential for growth of S. pombe, the galactosylation of protein is required for maintenance of normal cell shape, sexual agglutination, and tolerance toward various drugs. Cell surface glycoproteins play a key role in flocculation and filamentous invasive growth in yeasts. We identified fission yeast gsf2+, encoding a flocculin that binds to galactose residues located on cell surface glycoconjugates. Flocculation and invasive growth of S. pombe is tightly controlled by gsf2+ expression. Furthermore, pyruvylation of galactose residues negatively regulates flocculation by capping galactose residues in N-linked galactomannan. S. pombe appears to have a unique galactose-specific recognition system in which Gsf2p/flocculin plays an essential role in mediating cell-cell interactions. ©2011 FCCA.

    DOI: 10.4052/tigg.24.24

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  • Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe Reviewed

    Matsuzawa T., Morita T., Tanaka N., Tohda H., Takegawa K.

    Molecular Microbiology   82 ( 6 )   1531 - 1544   2011.12   ISSN:0950382X

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    Summary: Although various mutant strains of the fission yeast Schizosaccharomyces pombe exhibit non-sexual flocculation, little is known about the mechanistic basis for this phenomenon, nor have genes encoding the implicated flocculin been identified. In the budding yeast Saccharomyces cerevisiae, the transcription factor Flo8 controls expression of some of the genes involved in non-sexual flocculation. We have found that overexpression of S. cerevisiae FLO8 induced non-sexual flocculation in S. pombe. This non-sexual flocculation was Ca 2+-dependent, and was inhibited by addition of galactose, but not by mannose, glucose or sucrose. In the FLO8-overexpressing strain, a gene designated gsf2 + (galactose-specific flocculation) was specifically induced. The gsf2 + gene was also highly expressed in lkh1Δ, tup12Δ and gsf1 mutants, all of which exhibited non-sexual flocculation dependent on gsf2 +. We show that the N-terminal region of Gsf2 recognizes galactose in mediating cell-cell interaction. Disruption of gsf2 + also abolished the adhesion phenotype and invasive growth of the wild-type strain cultured in low ammonium medium. The newly identified flocculin Gsf2 in fission yeast was not only required for non-sexual flocculation but was also required for adhesion and filamentous growth through recognition of galactose residues on cell surface glycoconjugates. © 2011 Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-2958.2011.07908.x

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  • Genome sequence of the white Koji mold, Aspergillus kawachii IFO4308 used for brewing the Japanese distilled spirit, Shochu. Reviewed International journal

    Futagami T, Mori K, Yamashita A, Wada S, Kajiwara Y, Takashita H, Omori T, Takegawa K, Tashiro K, Kuhara S, and Goto M

    Eukaryotic Cell   2011.11

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  • Genome announcement: Genome sequence of the white Koji mold aspergillus kawachii IFO 4308, used for brewing the Japanese distilled spirit shochu Reviewed

    Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.

    Eukaryotic Cell   10 ( 11 )   1586 - 1587   2011.11   ISSN:15359778

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    The filamentous fungus Aspergillus kawachii has traditionally been used for brewing the Japanese distilled spirit shochu. A. kawachii characteristically hyperproduces citric acid and a variety of polysaccharide glycoside hydrolases. Here the genome sequence of A. kawachii IFO 4308 was determined and annotated. Analysis of the sequence may provide insight into the properties of this fungus that make it superior for use in shochu production, leading to the further development of A. kawachii for industrial applications. © 2011, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/EC.05224-11

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  • Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion. Reviewed

    Hosomi A, Nakase M, Takegawa K

    Journal of Bioscience and Bioengineering   2011.10

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  • A Myxococcus xanthus bacterial tyrosine kinase, BtkA, is required for the formation of mature spores. Reviewed International journal

    Kimura Y, Yamashita S, Mori Y, Kitajima Y, and Takegawa K

    Journal of Bacteriology   2011.10

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  • Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains Reviewed

    Futagami T., Okamoto F., Hashimoto H., Fukuzawa K., Fukuzawa K., Nazir K.H.M.N.H., Wada E., Suyama A., Takegawa K., Goto M., Nakamura K., Furukawa K.

    Bioscience, Biotechnology and Biochemistry   75 ( 7 )   1268 - 1274   2011.8   ISSN:09168451

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    A microbial consortium that reductively dechlorinates trichloroethene, cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with methanogenesis was enriched from chloroethene-contaminated soil from Japan. Dechlorination activity was maintained for over 4 years. Using quantitative polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were detected. Their growth and dechlorination activities were classified into two types: one that grows by converting cis-DCE to ethene and the other that grows by converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC reductive dehalogenases were detected. Inhibitors of methanogenesis (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of cis-DCE and of VC respectively. These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides".

    DOI: 10.1271/bbb.110028

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  • Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple Dehalococcoides strains. Reviewed

    Futagami T, Okamoto F, Hashimoto H, Fukuzawa K, Higashi K, Nazir K, Wada E, Suyama A, Takegawa K, Goto M, Nakamura K, Furukawa K

    Bioscience, Biotechnology, and Biochemistry   2011.7

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  • Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus. Reviewed International journal

    Kimura Y, Mori Y, Ina Y, Takegawa K

    Journal of Bacteriology   2011.5

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  • Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus Reviewed

    Kimura Y., Mori Y., Ina Y., Takegawa K.

    Journal of Bacteriology   193 ( 10 )   2657 - 2661   2011.5   ISSN:00219193

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    A protein phosphatase, designated Pph3, from Myxococcus xanthus showed the enzymatic characteristics of PP2C-type serine/threonine protein phosphatases, which are metal ion-dependent, okadaic acid-insensitive protein phosphatases. The pph3 mutant under starvation conditions formed immature fruiting bodies and reduced sporulation. © 2011, American Society for Microbiology.

    DOI: 10.1128/JB.01357-10

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  • Analysis of processing and maturation mechanism of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe. Reviewed International journal

    Mukaiyama H, Iwaki T, Idiris A, Takegawa K

    Applied Microbiology and Biotechnology   2011.4

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  • Processing and maturation of carboxypeptidase y and alkaline phosphatase in Schizosaccharomyces pombe Reviewed

    Mukaiyama H., Iwaki T., Idiris A., Takegawa K.

    Applied Microbiology and Biotechnology   90 ( 1 )   203 - 213   2011.4   ISSN:01757598

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    Schizosaccharomyces pombe carboxypeptidase Y (CPY) is synthesized as a zymogen and transported into the vacuole where maturation and activation occurs. The 110-kDa S. pombe CPY precursor is processed twice and finally converted to a mature form consisting of polypeptides of approximately 19 and 32 kDa linked by a single disulfide bond. In Saccharomyces cerevisiae, maturation of CPY occurs mostly through the activity of vacuolar aspartyl protease Pep4p, whereas a Pep4p homolog has not been found in the S. pombe genome database. Based on analysis of protease-deficient mutants, we found that S. pombe CPY was not able to be processed or activated in isp6Δpsp3Δ double disruptants. Both Isp6p and Psp3p are subtilase-type serine proteases with related sequences. Moreover, alkaline phosphatase of S. pombe was found to be localized at the vacuolar membrane and was also unprocessed in isp6Δpsp3Δ double disruptants. Vacuolar localization of GFP-fused Isp6p and Psp3p was determined by fluorescence microscopy. These results suggest that the two serine proteases Isp6p and Psp3p are functional in the vacuole and are involved in proteolytic processing of vacuolar proteins. © 2010 Springer-Verlag.

    DOI: 10.1007/s00253-010-3031-3

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  • Atg22p, a vacuolar membrane protein involved in the amino acid compartmentalization of schizosaccharomyces pombe Reviewed

    Sugimoto N., Iwaki T., Chardwiriyapreecha S., Shimazu M., Kawano M., Sekito T., Takegawa K., Kakinuma Y.

    Bioscience, Biotechnology and Biochemistry   75 ( 2 )   385 - 387   2011.3   ISSN:09168451

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    The fission yeast Schizosaccharomyces pombe has a homolog of the budding yeast Atg22p, which is involved in spore formation (Mukaiyama H. et ah, Microbiology, 155, 3816-3826 (2009)). GFP-tagged Atg22p in the fission yeast was localized to the vacuolar membrane. Upon disruption of atg22, the amino acid levels of the cellular fraction as well as the vacuolar fraction decreased. The uptake of several amino acids, such as lysine, histidine, and arginine, was impaired in atg22A cells. S. pombe Atg22p plays an important role in the compartmentalization of amino acids.

    DOI: 10.1271/bbb.100747

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  • Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions Reviewed International journal

    2011.3

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  • Atg22p, a vacuolar membrane protein involved in amino acid compartmentalization of Schizosaccharomyces pombe. Reviewed

    Sugimoto N, Iwaki T, Chardwiriyapreecha S, Shimazu M, Sekito T, Takegawa K, Kakinuma Y

    Bioscience, Biotechnology, and Biochemistry   2011.3

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  • New insights into galactose metabolism by Schizosaccharomyces pombe: isolation of a galactose-assimilating mutant. Reviewed International journal

    Matsuzawa T, Fujita Y, Tanaka N, Tohda H, Itadani A, Takegawa K

    Journal of Bioscience and Bioengineering   2011.2

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  • New insights into galactose metabolism by Schizosaccharomyces pombe: Isolation and characterization of a galactose-assimilating mutant Reviewed

    Matsuzawa T., Fujita Y., Tanaka N., Tohda H., Itadani A., Takegawa K.

    Journal of Bioscience and Bioengineering   111 ( 2 )   158 - 166   2011.2   ISSN:13891723

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    The fission yeast Schizosaccharomyces pombe cannot use galactose as a carbon or energy source, and little is known about galactose metabolism in this species. Here we report isolation of a galactose-assimilating mutant that grows on a medium containing galactose as a sole carbon source through use of a proofreading-deficient DNA polymerase δ variant encoded by cdc6-1. Based on comparative analysis of gene expression profiles in the wild-type and the mutant (FG2-8), we found that SPBPB2B2.10c (gal7+), SPBPB2B2.12c (gal10+) and SPBPB2B2.13 (gal1+), homologous to Saccharomyces cerevisiae GAL7, GAL10 and GAL1, respectively, and SPBPB2B2.08, SPBPB2B2.09c, and SPBPB2B2.11 that localize close to the gal genes, were highly expressed and dramatically induced by addition of galactose. The gal7δ strain, carrying an integrated ura4+ marker at the gal7+ locus, grew on 5-fluoroorotic acid (5-FOA)-containing medium. In contrast, the FG2-8 gal7δ strain could not grow on 5-FOA medium. In addition, expression of gal7+, SPBPB2B2.13, gal10+ and gal1+ genes increased in the wild-type strain when carried on a vector, and these transformants grew on galactose medium. We suggest that gal7+, gal10+, and gal1+ are localized close to a chromosomal terminal repressed by gene silencing in S. pombe. In contrast, gene silencing was defective in the FG2-8 strain making galactose assimilation possible. © 2010 The Society for Biotechnology, Japan.

    DOI: 10.1016/j.jbiosc.2010.10.007

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  • Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in arabidopsis Reviewed

    Hirano T., Matsuzawa T., Takegawa K., Sato M.H.

    Plant Physiology   155 ( 2 )   797 - 807   2011.2   ISSN:00320889

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    In eukaryotic cells, PtdIns 3,5-kinase, Fab1/PIKfyve produces PtdIns (3,5) P2 from PtdIns 3-P, and functions in vacuole/ lysosome homeostasis. Herein, we show that expression of Arabidopsis (Arabidopsis thaliana) FAB1A/B in fission yeast (Schizosaccharomyces pombe) fab1 knockout cells fully complements the vacuole morphology phenotype. Subcellular localizations of FAB1A and FAB1B fused with green fluorescent protein revealed that FAB1A/B-green fluorescent proteins localize to the endosomes in root epidermal cells of Arabidopsis. Furthermore, reduction in the expression levels of FAB1A/B by RNA interference impairs vacuolar acidification and endocytosis. These results indicate that Arabidopsis FAB1A/B functions as PtdIns 3,5-kinase in plants and in fission yeast. Conditional knockdown mutant shows various phenotypes including root growth inhibition, hyposensitivity to exogenous auxin, and disturbance of root gravitropism. These phenotypes are observed also in the overproducing mutants of FAB1A and FAB1B. The overproducing mutants reveal additional morphological phenotypes including dwarfism, male-gametophyte sterility, and abnormal floral organs. Taken together, this evidence indicates that imbalanced expression of FAB1A/B impairs endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification, which causes pleiotropic developmental phenotypes mostly related to the auxin signaling in Arabidopsis. © 2010 American Society of Plant Biologists.

    DOI: 10.1104/pp.110.167981

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  • Identification of a gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica. Reviewed International journal

    Morita T, Ito E, Kitamoto HK, Fukuoka T, Imura T, Takegawa K, Kitamoto D

    Yeast   2010.11

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  • Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe. Reviewed International journal

    Ohashi T, Nakakita S, Sumiyoshi W, Takegawa K

    Journal of Biotechnology   2010.11

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  • Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe Reviewed

    Ohashi T., Nakakita S., Sumiyoshi W., Takegawa K.

    Journal of Biotechnology   150 ( 3 )   348 - 356   2010.11   ISSN:01681656

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    The early stages of N-linked glycosylation are highly conserved between fungal and mammalian cells. Such N-linked oligosaccharides are synthesized through the ordered assembly of a dolichyl pyrophosphate (Dol-PP)-linked Glc3Man9GlcNAc2 structure by the sequential actions of several glycosyltransferases located in the endoplasmic reticulum (ER). Of the glycosyltransferase genes, Saccharomyces cerevisiae ALG3 has been identified to encode the Dol-P-Man:Man5GlcNAc2-PP-Dol α1,3-mannosyltransferase, and an alg3 mutant has been shown to accumulate an Endo H-resistant M5B (Manα1,2-Manα1,2-Manα1,3(Manα1,6-)-Manβ1,4-GlcNAcβ1,4-GlcNAc) structure. Although Schizosaccharomyces pombe contains a homolog of the ALG3 gene (SPAC7D4.06c), the role of this gene in oligosaccharide biosynthesis is not at all clear. In this study, we deleted the alg3+ gene in the och1Δ mutant and analyzed the detailed oligosaccharide structures in alg3Δoch1Δ double mutant. The oligosaccharides were prepared from cell-surface glycoproteins by hydrazinolysis and fluorescent labeling with 2-aminopyridine. The labeled oligosaccharides were analyzed by high performance liquid chromatography, in combination with sequential glycosidase digestion and methylation analysis. These analyses revealed that the N-linked oligosaccharides of S. pombe alg3Δoch1Δ cells mainly consisted of two or three α-galactose-capped M5B structures. Finally, western blot analysis of recombinant human transferrin suggested that heterologously expressed glycoproteins in alg3Δoch1Δ cells have Endo H-resistant N-linked oligosaccharide structures similar to those of alg3Δoch1Δ cell-surface glycoproteins. © 2010 Elsevier B.V.

    DOI: 10.1016/j.jbiotec.2010.09.942

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  • Identification of the gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica Reviewed

    Morita T., Ito E., Kitamoto H., Takegawa K., Fukuoka T., Imura T., Kitamoto D.

    Yeast   27 ( 11 )   905 - 917   2010.11   ISSN:0749503X

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    The yeast Pseudozyma antarctica produces a large amount of glycolipid biosurfactants known as mannosylerythritol lipids (MELs), which show not only excellent surface-active properties but also versatile biochemical actions. To investigate the biosynthesis of MELs in the yeast, we recently reported expressed sequence tag (EST) analysis and estimated genes expressing under MEL production conditions. Among the genes, a contiguous sequence of 938 bp, PA_004, showed high sequence identity to the gene emt1, encoding an erythritol/mannose transferase of Ustilago maydis, which is essential for MEL biosynthesis. The predicted translation product of the extended PA_004 containing the two introns and a stop codon was aligned with Emt1 of U. maydis. The predicted amino acid sequence shared high identity (72%) with Emt1 of U. maydis, although the amino-terminal was incomplete. To identify the gene as PaEMT1 encoding an erythritol/mannose transferase of P. antarctica, the gene-disrupted strain was developed by the method for targeted gene disruption, using hygromycin B resistance as the selection marker. The obtained ΔPaEMT1 strain failed to produce MELs, while its growth was the same as that of the parental strain. The additional mannosylerythritol into culture allowed ΔPaEMT1 strain to form MELs regardless of the carbon source supplied, indicating a defect of the erythritol/mannose transferase activity. Furthermore, we found that MEL formation is associated with the morphology and low-temperature tolerance of the yeast. © 2010 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.1794

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  • Vba2p: A Vacuolar Membrane Protein Involving in Basic Amino Acid Transport in Schizosaccharomyces pombe Reviewed

    Sugimoto N, Iwaki T, Chardwiriyapreecha M, Shimazu S, Sekito T, Takegawa K, Kakinuma Y

    Bioscience, Biotechnology, and Biochemistry   2010.10

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  • Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe. Reviewed International journal

    Chardwiriyapreecha S, Mukaiyama H, Sekito T, Iwaki T, Takegawa K, Kakinuma Y

    FEBS Letters   2010.6

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  • The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe. Reviewed International journal

    Matsuzawa T, Ohashi T, Hosomi A, Tanaka N, Tohda H, Takegawa K

    Applied Microbiology and Biotechnology   2010.6

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  • Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe Reviewed

    Chardwiriyapreecha S., Mukaiyama H., Sekito T., Iwaki T., Takegawa K., Kakinuma Y.

    FEBS Letters   584 ( 11 )   2339 - 2345   2010.6   ISSN:00145793

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    We identified SPBC1685.07c of Schizosaccharomyces pombe as a novel vacuolar protein, Avt5p, with similarity to vacuolar amino acid transporters Avt5p from Saccharomyces cerevisiae. Avt5p localizes to the vacuolar membrane and upon disruption of avt5, uptake of histidine, glutamate, tyrosine, arginine, lysine or serine was impaired. During nitrogen starvation, the transient increase of vacuolar lysine transport observed for wild-type cells still occurred in the mutant cells, however, uptake of glutamate did not significantly increase in response to nitrogen starvation. Our results show that under diverse growth conditions Avt5p is involved in vacuolar transport of a selective set of amino acids. © 2010 Federation of European Biochemical Societies.

    DOI: 10.1016/j.febslet.2010.04.012

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  • Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe Reviewed

    Nakase M., Tani M., Morita T., Kitamoto H.K., Kashiwazaki J., Nakamura T., Hosomi A., Tanaka N., Takegawa K.

    Journal of Cell Science   123 ( 9 )   1578 - 1587   2010.5   ISSN:00219533

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    In Saccharomyces cerevisiae, three classes of sphingolipids contain myo-inositol - inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP) 2C]. No fission yeast equivalent of Ipt1p, the inositolphosphotransferase that synthesizes M(IP)2C from MIPC, has been found in the Schizosaccharomyces pombe genome. Analysis of the sphingolipid composition of wild-type cells confirmed that MIPC is the terminal and most abundant complex sphingolipid in S. pombe. Three proteins (Sur1p, Csg2p and Csh1p) have been shown to be involved in the synthesis of MIPC from IPC in S. cerevisiae. The S. pombe genome has three genes (SPAC2F3.01, SPCC4F11.04c and SPAC17G8.11c) that are homologues of SUR1, termed imt1+, imt2 + and imt3+, respectively. To determine whether these genes function in MIPC synthesis in S. pombe, single and multiple gene disruptants were constructed. Single imt disruptants were found to be viable. MIPC was not detected and IPC levels were increased in the triple disruptant, indicating that the three SUR1 homologues are involved in the synthesis of MIPC. GFP-tagged Imt1p, Imt2p and Imt3p localized to Golgi apparatus membranes. The MIPC-deficient mutant exhibited pleiotropic phenotypes, including defects in cellular and vacuolar morphology, and in localization of ergosterols. MIPC seemed to be required for endocytosis of a plasma-membrane-localized amino acid transporter, because sorting of the transporter from the plasma membrane to the vacuole was severely impaired in the MIPC-deficient mutant grown under nitrogen-limiting conditions. These results suggest that MIPC has multiple functions not only in the maintenance of cell and vacuole morphology but also in vesicular trafficking in fission yeast.

    DOI: 10.1242/jcs.059139

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  • Critical roles of mucin 1 glycosylation by transactivated polypeptide N-acetylgalactosaminyltransferase 6 in mammary carcinogenesis Reviewed

    Park J.H., Nishidate T., Kijima K., Ohashi T., Takegawa K., Fujikane T., Hirata K., Nakamura Y., Katagiri T.

    Cancer Research   70 ( 7 )   2759 - 2769   2010.4   ISSN:00085472

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    The structure of O-glycosylated proteins is altered in breast cancer cells, but the mechanisms of such an aberrant modification have been largely unknown. We here report critical roles of a novel druggable target, polypeptide N-acetylgalactosaminyltransferase 6 (GALNT6), which is upregulated in a great majority of breast cancers and encodes a glycosyltransferase responsible for initiating mucin-type O-glycosylation. Knockdown of GALNT6 by small interfering RNA significantly enhanced cell adhesion function and suppressed the growth of breast cancer cells. Western blot and immunostaining analyses indicated that wild-type GALNT6 protein could glycosylate and stabilize an oncoprotein mucin 1 (MUC1), which was upregulated with GALNT6 in breast cancer specimens. Furthermore, knockdown of GALNT6 or MUC1 led to similar morphologic changes of cancer cells accompanied by the increase of cell adhesion molecules β-catenin and E-cadherin. Our findings implied that overexpression of GALNT6 might contribute to mammary carcinogenesis through aberrant glycosylation and stabilization of MUC1 and that screening of GALNT6 inhibitors would be valuable for the development of novel therapeutic modalities against breast cancer. © 2010 American Association for Cancer Research.

    DOI: 10.1158/0008-5472.CAN-09-3911

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  • Autophagy in the fission yeast Schizosaccharomyces pombe. Invited Reviewed International journal

    Mukaiyama H, Nakase M, Nakamura T, Kakinuma Y, Takegawa K

    FEBS Letters   2010.4

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  • Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe. Reviewed International journal

    Mukaiyama H, Tohda H, Takegawa K

    Applied Microbiology and Biotechnology   2010.4

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  • A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling. Reviewed

    Hasehira K, Nakakita S, Miyanishi N, Sumiyoshi W, Hayashi S, Takegawa K, Hirabayashi J

    Journal of Biochemistry   2010.4

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  • Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe Reviewed

    Mukaiyama H., Tohda H., Takegawa K.

    Applied Microbiology and Biotechnology   86 ( 4 )   1135 - 1143   2010.4   ISSN:01757598

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    Although the fission yeast Schizosaccharomyces pombe has been used for high-level heterologous protein production, the productivity of secreted human serum transferrin (hTF) has been low, presumably, because the protein harbors twenty disulfide bonds and two Nglycosylation sites. In the present study, we found that overexpression of endogenous putative protein disulfide isomerase (PDI) improved productivity. Whole genome sequence analysis of S. pombe revealed five putative PDI genes and overexpression of two of them, SPAC17H9.14c and SPBC3D6.13c (SpPdi2p or SpPdi3p, respectively), significantly improved the productivity of secreted hTF. GFP-fused SpPdi2p and SpPdi3p were found to localize to the endoplasmic reticulum. Co-overexpression of SpPdi2p or SpPdi3p with hTF coupled with modifications to the growth medium reported in our previous study were able to increase the level of secreted hTF approximately 30-fold relative to conventional conditions. © 2009 Springer-Verlag.

    DOI: 10.1007/s00253-009-2393-x

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  • A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling Reviewed

    Hasehira K., Nakakita S.I., Miyanishi N., Sumiyoshi W., Hayashi S., Takegawa K., Hirabayashi J.

    Journal of Biochemistry   147 ( 4 )   501 - 509   2010.4   ISSN:0021924X

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    Rare sugars are defined as monosaccharides with extremely low natural abundances. Their natural distributions and biological functions remain to be clarified. To establish a robust analytical system that can separate, identify and quantify rare sugars, 12 d-hexoses - including five rare aldohexoses and three rare ketohexoses - were labelled with 2-aminobenzamide (2-AB), and the fluorescently tagged monosaccharides were separated by high-performance liquid chromatography (HPLC). Because the ketohexoses were much less reactive than were the aldohexoses, the reaction conditions were optimized to achieve the maximum yields (>75) for both aldohexoses and ketohexoses. The calibration curve determined for the rare ketohexose, d-psicose (Psi), was linear between 1 pmol and 1 mol and had a correlation coefficient of 0.9999. Using the developed method, the psicose content in a leaf of Itea virginica, in which the presence of psicose has previously been reported, was found to be 2.7 mg psicose/g leaf. The result proved feasibility of the method even for natural products. Because the system is a comprehensive tool, it should help answer questions concerning the biosyntheses and functions of rare hexose sugars. © 2009 The Authors.

    DOI: 10.1093/jb/mvp199

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  • Autophagy in the fission yeast Schizosaccharomyces pombe Reviewed

    Mukaiyama H., Nakase M., Nakamura T., Kakinuma Y., Takegawa K.

    FEBS Letters   584 ( 7 )   1327 - 1334   2010.4   ISSN:00145793

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    Autophagy is a non-selective degradation process in eukaryotic cells. The genome sequence of the fission yeast Schizosaccharomyces pombe has revealed that many of the genes required for autophagy are common between the fission yeast and budding yeast, suggesting that the basic machinery of autophagy is conserved between these species. Autophagy in fission yeast is specifically induced by nitrogen starvation based on monitoring a GFP-Atg8p marker. Upon nitrogen starvation, fission yeast cells exit the vegetative cell cycle and initiate sexual differentiation to produce spores. Most of the nitrogen used for de novo protein synthesis during sporulation derives from the autophagic protein degradation system. This review focuses on the recent advances in the role of autophagy in fission yeast. © 2009 Federation of European Biochemical Societies.

    DOI: 10.1016/j.febslet.2009.12.037

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  • Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast Reviewed

    Suzuki S., Matsuzawa T., Nukigi Y., Takegawa K., Tanaka N.

    Microbiology   156 ( 3 )   708 - 718   2010.3   ISSN:13500872

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    Schizosaccharomyces species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, uge1+ and gal10+. A strain deleted for uge1+ exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2% glucose), indicating that Uge1p is a major UDP-glucose/-galactose 4-epimerase under these growth conditions. In contrast, gal10+ was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1% glucose and 2% glycerol), but not in galactose-containing medium. In a uge1Δgal10Δ strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of gal7+, encoding galactose-1-phosphate uridylyltransferase, in the uge1Δ gal10Δ strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in Sch. pombe. © 2010 SGM.

    DOI: 10.1099/mic.0.035279-0

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  • Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast. Reviewed International journal

    Suzuki S, Matsuzawa T, Nukigi Y, Takegawa K, Tanaka N

    Microbiology   2010.3

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  • Engineering of protein secretion in yeast: strategies and impact on protein production. Invited Reviewed International journal

    Idiris A, Tohda H, Kumagai H, Takegawa K

    Applied Microbiology and Biotechnology   2010.3

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  • N- and O-linked oligosaccharides completely lack galactose residues in the gms1och1 mutant of Schizosaccharomyces pombe Reviewed

    Ohashi T., Takegawa K.

    Applied Microbiology and Biotechnology   86 ( 1 )   263 - 272   2010.3   ISSN:01757598

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    Unlike their counterparts in budding yeast Saccharomyces cerevisiae, the glycoproteins of Schizosaccharomyces pombe contain, in addition to α-d-mannose (Man), a large number of α-d-galactose (Gal) residues. In both yeasts, large outer chains are attached to the oligosaccharide cores of glycoproteins during export via Golgi. Formation of the yeast-specific large outer chain is initiated by α-1,6-mannosylatransferase encoded by the och1 + gene, the disruption of which blocked outer chain elongation. We previously reported that N-linked oligosaccharide structures of S. pombe och1Δ mutant consisted of Gal2-6Man9GlcNAc 2 with α-linked Gal residues attached to the core oligosaccharide moiety. The disruption of gms1 +, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, abolished cell surface galactosylation in S. pombe. In this study, we constructed a gms1Δoch1Δ double mutant and determined the N- and O-linked oligosaccharide structures present on the cell surface. Oligosaccharides were liberated from glycoproteins by hydrazinolysis and labeled with the fluorophore, 2-aminopyridine. The pyridylaminated N-linked oligosaccharides were analyzed by high-performance liquid chromatography in combination with α1,2-mannosidase digestion and partial acetolysis. These analyses revealed that the N-linked oligosaccharides of gms1Δoch1Δ cells consisted of α1,2-linked Man-extended core oligosaccharides (Man8-12GlcNAc2) from which the fission yeast-specific α-linked Gal residues were completely absent. © 2009 Springer-Verlag.

    DOI: 10.1007/s00253-009-2297-9

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  • Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus Reviewed

    Kimura Y., Kawasaki S., Yoshimoto H., Takegawa K.

    Journal of Bacteriology   192 ( 5 )   1467 - 1470   2010.3   ISSN:00219193

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    Glycine sarcosine methyltransferase (Gsm) and sarcosine dimethylglycine methyltransferase (Sdm) catalyze glycine betaine synthesis from glycine. Disruption of the M. xanthus gsmA (MXAN 7068) or sdmA (MXAN 3190) gene, encoding Gsm or Sdm homologue proteins, respectively, generated mutants that exhibited a longer lag period of growth and delayed spore germination under osmostress. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/JB.01118-09

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  • Structure of the N- and O-linked oligosaccharides that show complete loss of galactose residues from gms1och1 mutant of Schizosaccharomyces pombe. Reviewed International journal

    Ohashi T, Takegawa K

    Applied Microbiology and Biotechnology   2010.2

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  • Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus. Reviewed International journal

    Kimura Y, Kawasaki S, Yoshimoto H, Takegawa K

    Journal of Bacteriology   2010.2

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  • Enhanced protein secretion from the multiprotease-deletion fission yeast by modification of vacuolar protein sorting pathway. Reviewed International journal

    Idiris A, Tohda H, Kumagai H, Giga-Hama Y, Takegawa K

    Applied Microbiology and Biotechnology   2010.1

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  • Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway Reviewed

    Idiris A., Tohda H., Sasaki M., Okada K., Kumagai H., Giga-Hama Y., Takegawa K.

    Applied Microbiology and Biotechnology   85 ( 3 )   667 - 677   2010.1   ISSN:01757598

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    Previously, we achieved approximately 30-fold enhanced secretion of the protease-sensitive model protein human growth hormone (hGH) by multiple gene deletion of seven obstructive proteases in the fission yeast Schizosaccharomyces pombe. However, intracellular retention of secretory hGH was found in the resultant multiprotease-deficient strains. As a solution, genetic modification of the intracellular trafficking pathway that is related to intracellular retention of hGH was attempted on a protease octuple deletant strain. Vacuolar accumulation of the intracellularly retained hGH was identified by secretory expression of hGH fused with EGFP, and three vacuolar protein sorting (vps)-deficient strains, vps10Δ, vps22Δ, and vps34Δ, were determined on account of their hGH secretion efficiency. The mutant vps10Δ was found to be effective for hGH secretion, which suggested a role for vps10 in the vacuolar accumulation of the intracellularly retained hGH. Finally, vps10 deletion was performed on the protease octuple deletant strain, which led to an approximately 2-fold increase in hGH secretion. This indicated the possible application of secretory-pathway modification and multiple protease deletion for improving heterologous protein secretion from the fission yeast S. pombe. © 2009 Springer-Verlag.

    DOI: 10.1007/s00253-009-2151-0

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  • Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation Reviewed

    Mukaiyama H., Kajiwara S., Hosomi A., Giga-Hama Y., Tanaka N., Nakamura T., Takegawa K.

    Microbiology   155 ( 12 )   3816 - 3826   2009.12   ISSN:13500872

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    Autophagy is triggered when organisms sense radical environmental changes, including nutritional starvation. During autophagy, cytoplasmic components, including organelles, are enclosed within autophagosomes and are degraded upon lysosome-vacuole fusion. In this study, we show that processing of GFP-tagged Atg8 can serve as a marker for autophagy in the fission yeast Schizosaccharomyces pombe. Using this marker, 13 Atg homologues were also found to be required for autophagy in fission yeast. In budding yeast, autophagy-deficient mutants are known to be sterile, whereas in fission yeast we found that up to 30% of autophagy-defective cells with amino acid auxotrophy were able to recover sporulation when an excess of required amino acids was supplied. Furthermore, we found that approximately 15% of the autophagy-defective cells were also able to sporulate when a prototrophic strain was subjected to nitrogen starvation, which suggested that fission yeast may store sufficient intracellular nitrogen to allow partial sporulation under nitrogen-limiting conditions, although the majority of the nitrogen source is supplied by autophagy. Monitoring of the sporulation process revealed that the process was blocked non-specifically at various stages in the atg1D and atg12D mutants, possibly due to a shortage of amino acids. Taking advantage of this partial sporulation ability of fission yeast, we sought evidence for the existence of a recycling system for nitrogen sources during starvation. © 2009 SGM.

    DOI: 10.1099/mic.0.034389-0

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  • Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation. Reviewed International journal

    Mukaiyama H, Kajiwara S, Hosomi A, Giga-Hama Y, Tanaka N, Namkamura T, Takegawa K

    Microbiology   2009.12

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  • Interaction between a Ser/Thr protein kinase, SpkA, and a cAMP-dependent protein kinase regulatory subunit homolog, CbpB, from Myxococcus xanthus. Reviewed

    Kimura Y, Kakemizu A, Matsubara Y, Takegawa K

    The Journal of General and Applied Microbiology   2009.12

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  • Interaction between a Ser/Thr protein kinase, SpkA, and a cAMP-dependent protein kinase regulatory subunit homolog, CbpB, from Myxococcus xanthus Reviewed

    Kimura Y., Kakemizu A., Matsubara Y., Takegawa K.

    Journal of General and Applied Microbiology   55 ( 6 )   499 - 502   2009.12   ISSN:00221260

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    DOI: 10.2323/jgam.55.499

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  • Dextran sodium sulfate is effective enhancer for secretion of recombinant human transferrin in Schizosaccharomyces pombe. Reviewed International journal

    Mukaiyama H, Tohda H, Giga-Hama Y, Takegawa K

    Applied Microbiology and Biotechnology   2009.11

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  • The dynamin-related protein Vps1 regulates vacuole fisson, fusion and tubulation in the fission yeast, S. pombe. Reviewed International journal

    Rothlisberger S, Jourdain I, Johnson C, Takegawa K, Hyams JS

    Fungal Genetics and Biology   2009.11

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  • Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe Reviewed

    Mukaiyama H., Giga-Hama Y., Tohda H., Takegawa K.

    Applied Microbiology and Biotechnology   85 ( 1 )   155 - 164   2009.11   ISSN:01757598

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    The effect of medium supplementation on heterologous production of human serum transferrin (hTF) in the fission yeast Schizosaccharomyces pombe has been investigated. The productivity of recombinant hTF was low in wild-type S. pombe cells. To overcome this impediment, culture media supplements were screened for their ability to improve secretion of hTF. Casamino acids (CAA), which have been reported to increase heterologous protein productivity in Pichia pastoris, improved the secretion hTF by more than fourfold. An anion surfactant deoxycholate or polyethylene glycol also improved the secretion hTF. Interestingly, dextran sodium sulfate (DSS), a poly-anion surfactant, was found to enhance production of secreted hTF better than any other supplement tested. Addition of DSS in the presence of 2% CAA exhibited a synergistic effect on increasing hTF secretion, resulting in an increase of about sevenfold relative to conventional conditions. Cell growth was not found to be affected by the addition of DSS or CAA. DSS may act as a surfactant and may also facilitate the anchoring of liposomes, and these properties may contribute to efficient secretion or exocytosis through the plasma membrane. © 2009 Springer-Verlag.

    DOI: 10.1007/s00253-009-2130-5

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  • Protein O-mannosyltransferase B and C support hyphal development and differentiation in Aspergillus nidulans. Reviewed International journal

    Goto M, Harada Y, Oka T, Matsumoto S, Takegawa K, Furukawa K

    Eukaryotic Cell   2009.10

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  • Protein O-mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans Reviewed

    Goto M., Harada Y., Oka T., Matsumoto S., Takegawa K., Furukawa K.

    Eukaryotic Cell   8 ( 10 )   1465 - 1474   2009.10   ISSN:15359778

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    Aspergillus nidulans possesses threepmt genes encoding protein O-D-mannosyltransferases (Pmt). Previously, we reported that PmtA, a member of the PMT2 subfamily, is involved in the proper maintenance of fungal morphology and formation of conidia (T. Oka, T. Hamaguchi, Y. Sameshima, M. Goto, and K. Furukawa, Microbiology 150:1973-1982, 2004). In the present paper, we describe the characterization of the pmtA paralogues pmtB and pmtC. PmtB and PmtC were classified as members of the PMT1 and PMT4 subfamilies, respectively. A pmtB disruptant showed wild-type (wt) colony formation at 30°C but slightly repressed growth at 42°C. Conidiation of the pmtB disruptant was reduced to approximately 50% of that of the wt strain; in addition, hyperbranching of hyphae indicated that PmtB is involved in polarity maintenance. ApmtA and pmtB double disruptant was viable but very slow growing, with morphological characteristics that were cumulative with respect to either single disruptant. Of the three single pmt mutants, the pmtC disruptant showed the highest growth repression; the hyphae were swollen and frequently branched, and the ability to form conidia under normal growth conditions was lost. Recovery from the aberrant hyphal structures occurred in the presence of osmotic stabilizer, implying that PmtC is responsible for the maintenance of cell wall integrity. Osmotic stabilization at 42°C further enabled the pmtC disruptant to form conidiophores and conidia, but they were abnormal and much fewer than those of the wt strain. Apart from the different, abnormal phenotypes, the three pmt disruptants exhibited differences in their sensitivities to antifungal reagents, mannosylation activities, and glycoprotein profiles, indicating that PmtA, PmtB, and PmtC perform unique functions during cell growth. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/EC.00371-08

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  • Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses. Reviewed International journal

    Nakakita S, Hasehira K, Hosokawa T, Tokuda M, Izumori K, Takegawa K, Hirabayashi J

    Journal of Chromatography A   2009.7

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  • Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system. Invited Reviewed International journal

    Takegawa K, Tohda H, Sasaki M, Idiris A, Ohashi T, Mukaiyama H, Giga-Hama Y, Kumagai H

    Biotechnology and Applied Biochemistry   2009.7

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  • Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses Reviewed

    Nakakita S.i., Hasehira K., Hosokawa T., Tokuda M., Izumori K., Takegawa K., Hirabayashi J.

    Journal of Chromatography A   1216 ( 26 )   5112 - 5115   2009.6   ISSN:00219673

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    A rare sugar is considered to be a monosaccharide rarely found in nature. To investigate their natural distribution and biological roles, a robust analytical system must be used to isolate, identify, and quantify them. Herein, we report the development of such a system that can specifically quantify and chromatographically separate four aldopentoses and eight aldohexoses tagged with 2-aminopyridine. Purified monosaccharides derivatized with a pyridylamino moiety (PA-monosaccharides) are first chromatographed over a high-performance anion-exchange resin. But, because two of the PA-aldohexoses used in this study, PA-talose and PA-idose, co-elute with the common saccharides, PA-glucose and PA-mannose, respectively, a second chromatographic step, reversed-phase high-performance liquid chromatography, is used to completely separate them. Thus, as shown by the results of this study, chromatographic separation of PA-monosaccharides is achievable and provides a quantitative measurement of common and rare isomeric aldopentoses and aldohexoses. © 2009 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.chroma.2009.04.072

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  • The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides. Reviewed

    Ohashi T, Ikeda Y, Tanaka N, Nakakita S, Natsuka S, Giga-Hama Y, Takegawa K

    Bioscience, Biotechnology, and Biochemistry   2009.2

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  • Identification and characterization of a gene required for α1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe Reviewed

    Ikeda Y., Ohashi T., Tanaka N., Takegawa K.

    FEMS Yeast Research   9 ( 1 )   115 - 125   2009.2   ISSN:15671356

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    The KTR α1,2-mannosyltransferase gene family of Saccharomyces cerevisiae is responsible not only for outer-chain modifications of N-linked oligosaccharides but also for elongation of O-linked mannose residues. To identify genes involved in the elongation step of O-linked oligosaccharide chains in Schizosaccharomyces pombe, we characterized six genes, omh1 +-omh6+, that share significant sequence similarity to the S. cerevisiae KTR family. Six deletion strains were constructed, each carrying a single disrupted omh allele. All strains were viable, indicating that none of the omh genes was essential. Heterologous expression of a chitinase from S. cerevisiae in the omh mutants revealed that O-glycosylation of chitinase had decreased in omh1Δ cells, but not in the other mutants, indicating that the other omh genes do not appear to be required for O-glycan synthesis. Addition of the second α1,2-linked mannose residue was blocked in omh1Δ cells. An Omh1-GFP fusion protein was found to be localized in the Golgi apparatus. These results indicate that Omh1p plays a major role in extending α1,2-linked mannose in the O-glycan pathway in S. pombe. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

    DOI: 10.1111/j.1567-1364.2008.00458.x

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  • Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3′,5′- and 2′,3′-cAMP phosphodiesterase, and phosphatase activities Reviewed

    Kimura Y., Okazaki N., Takegawa K.

    FEBS Letters   583 ( 2 )   443 - 448   2009.1   ISSN:00145793

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    Myxococcus xanthus PdeA and PdeB, enzymes homologous to class III 3′,5′-cyclic nucleotide phosphodiesterases, hydrolyzed 3′,5′- and 2′,3′-cyclic AMP (cAMP) to adenosine, and also demonstrated phosphatase activity toward nucleoside 5′-tri-, 5′-di-, 5′- and 3′-monophosphates with highest activities for nucleoside 5′-monophosphates. The substrate specificities of PdeA and PdeB show no similarity to that of any known cNMP phosphodiesterase, nucleotidase, or phosphatase. The enzyme activities of PdeA and PdeB were stimulated by 50 μM Mn2+ or Co2+. The Km values of PdeA and PdeB for 3′,5′-cAMP, 2′,3′-cAMP, 5′-ATP, and 5′-AMP were in the low micromolar range (1.4-12.5 μM). © 2008 Federation of European Biochemical Societies.

    DOI: 10.1016/j.febslet.2008.12.044

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  • Identification and characterization of a gene required for α1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe Reviewed International journal

    2009.1

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  • Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus. Reviewed

    Kimura Y, Kakemizu A, Matsubara Y, Takegawa K

    Journal of Bioscience and Bioengineering   2009.1

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  • Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3’,5’- and 2’,3’-cAMP phosphodiesterase, and phosphatase activities. Reviewed International journal

    2009.1

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  • Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus Reviewed

    Kimura Y., Kakemizu A., Matsubara Y., Takegawa K.

    Journal of Bioscience and Bioengineering   107 ( 1 )   10 - 15   2009.1   ISSN:13891723

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    Two Ser/Thr protein kinases, SpkA and SpkB, selected from Myxococcus xanthus based on amino acid sequence similarities with the catalytic subunits of cAMP-dependent protein kinases (PKA) were synthesized using a cell-free protein synthesis system. In various protein kinase assays, purified StkA and StkB showed their highest protein kinase activities in a PKA assay using the selective PKA substrate Kemptide and in a protein kinase C (PKC) assay using the selective PKC substrate neurogranin(28-43), respectively. SpkA had apparent Km values of 45 μM and 37 μM for Kemptide and ATP, respectively. Phosphorylation of Kemptide was inhibited by a specific PKA inhibitor peptide, PKI5-24, and the IC50 and Ki values for inhibition of the SpkA activity were 117 nM and 36 nM, respectively. © 2008 The Society for Biotechnology, Japan.

    DOI: 10.1016/j.jbiosc.2008.08.002

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  • Identification of the fnx1+ and fnx2+ genes for vacuolar amino acid transporters in Schizosaccharomyces pombe. Reviewed International journal

    Chardwiriyapreecha, S., Shimazu, M., Morita, T., Sekito, T., Akiyama, K., Takegawa, K., and Kakinuma, Y.

    FEBS Letters   2008.7

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  • Identification of the fnx1<sup>+</sup> and fnx2<sup>+</sup> genes for vacuolar amino acid transporters in Schizosaccharomyces pombe Reviewed

    Chardwiriyapreecha S., Shimazu M., Morita T., Sekito T., Akiyama K., Takegawa K., Kakinuma Y.

    FEBS Letters   582 ( 15 )   2225 - 2230   2008.6   ISSN:00145793

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    We have identified the Schizosaccharomyces pombe SPBC3E7.06c gene (fnx2+) from a homology search with the fnx1+ gene involving in G0 arrest upon nitrogen starvation. Green fluorescent protein-fused Fnx1p and Fnx2p localized exclusively to the vacuolar membrane. Uptake of histidine or isoleucine by S. pombe cells was inhibited by concanamycin A, a specific inhibitor of the vacuolar H+-ATPase. Amino acid uptake was also defective in the vacuolar ATPase mutant, suggesting that vacuolar compartmentalization is critical for amino acid uptake by whole cells. In both Δfnx1 and Δfnx2 mutant cells, uptake of lysine, isoleucine or asparagine was impaired. These results suggest that fnx1+ and fnx2+ are involved in vacuolar amino acid uptake in S. pombe. © 2008 Federation of European Biochemical Societies.

    DOI: 10.1016/j.febslet.2008.05.017

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  • Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-β-N-acetylglucosaminidases. Reviewed International journal

    2008.4

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  • PXA domain-containing protein Pxa1 is required for normal vacuole function and morphology in Schizosaccharomyces pombe Reviewed

    Hosomi A., Kawanishi Y.Y., Tanaka N., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   72 ( 2 )   548 - 556   2008.3   ISSN:09168451

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    PhoX homology (PX) domain-containing proteins play critical roles in vesicular trafficking, protein sorting, and lipid modification in eukaryotic cells. Several proteins with PX domains contain an associated domain termed PXA (PX-associated). Although PXA domain-containing proteins are required for some important cellular processes, the function of the PXA domain is unknown. We identified three PXA domain-containing proteins in Schizosaccharomyces pombe. S. pombe Pxa1p (SPAC5D6.07c) contained only the PXA domain, not the PX domain. To elucidate the role of the PXA domain in eukaryotic cells, we constructed and characterized a disruption mutant, pxa1. The pxa1 disruptant contained enlarged vacuoles and exhibited mislocalization of vacuolar carboxypeptidase Y (CPY). The conversion rate from pro- to mature-CPY was greatly impaired in pxa1 cells, and fluorescence microscopy indicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. The mutants were also deficient in vacuolar sorting of a multivesicular body (MVB) marker, a ubiquitin-GFP- carboxypeptidase S (Ub-GFP-CPS) fusion protein. Taken together, these results indicate that Pxa1 protein is required for normal vacuole function and morphology in S. pombe.

    DOI: 10.1271/bbb.70666

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  • Galactinol synthase gene of Coptis japonica is involved in berberine tolerance Reviewed

    Takanashi K., Shitan N., Sugiyama A., Kamimoto Y., Hamamoto M., Iwaki T., Takegawa K., Yazaki K.

    Bioscience, Biotechnology and Biochemistry   72 ( 2 )   398 - 405   2008.3   ISSN:09168451

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    Many plant secondary metabolites show strong biological activities and are potentially also toxic to plants, while plants producing such active compounds are usually insensitive to their own metabolites, suggesting that they have species-specific detoxification mechanisms. In order to clarify the detoxification mechanism of alkaloids, we used cultured cells of Coptis japonica, which are capable of producing a yellow benzylisoquinoline alkaloid, berberine, and accumulate it in the vacuole. Unlike other plant cells that do not produce berberine, C. japonica shows strong tolerance to this alkaloid. We established a fission yeast strain that was sensitive to berberine and performed functional screening using a C. japonica cDNA library. One cDNA clone, which conferred clear berberine tolerance, encoded galactinol synthase (CjGolS). The possible role of CjGolS in berberine tolerance is discussed.

    DOI: 10.1271/bbb.70495

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  • Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Reviewed International journal

    Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., and Takegawa, K.

    Microbiology   2008.3

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  • Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe Reviewed

    Iwaki T., Iefuji H., Hiraga Y., Hosomi A., Morita T., Giga-Hama Y., Takegawa K.

    Microbiology   154 ( 3 )   830 - 841   2008.3   ISSN:13500872

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    Sterols are a major class of membrane lipids in eukaryotes. In Schizosaccharomyces pombe, sterol 24-C-methyltransferase (Erg6p), C-8 sterol isomerase (Erg2p), C-5 sterol desaturase (Erg31p, Erg32p), C-22 sterol desaturase (Erg5p) and C-24 (28) sterol reductase (Sts1p/Erg4p) have been predicted, but not yet determined, to catalyse a sequence of reactions from zymosterol to ergosterol. Disruption mutants of these genes were unable to synthesize ergosterol, and most were tolerant to the polyene drugs amphotericin B and nystatin. Disruption of erg31+ or erg32+ did not cause ergosterol deficiency or tolerance to polyene drugs, indicating that the two C-5 sterol desaturases have overlapping functions. GFP-tagged DRM (detergent-resistant membrane)-associated protein Pma1p localized to the plasma membrane in ergΔ mutants. DRM fractionation revealed that the association between Pma1-GFP and DRM was weakened in erg6Δ but not in other erg mutants. Several GFP-tagged plasma membrane proteins were tested, and an amino acid permease homologue, SPBC359.03c, was found to mislocalize to intracellular punctate structures in the ergA mutants. These results indicate that these proteins are responsible for ergosterol biosynthesis in fission yeast, similar to the situation in Saccharomyces cerevisiae. Furthermore, in fission yeast, ergosterol is important for plasma membrane structure and function and for localization of plasma membrane proteins. © 2008 SGM.

    DOI: 10.1099/mic.0.2007/011155-0

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  • PXA domain-containing protein Pxa1 is required for normal vacuole function and morphology in Schizosaccharomyces pombe. Reviewed International journal

    Hosomi, A., Kawanishi, Y., Tanaka, N., and Takegawa, K.

    Bioscience, Biotechnology, and Biochemistry   2008.2

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  • Galactinol synthase genes of Coptis japonica are involved in confers berberine tolerance in yeast Reviewed

    Takanashi, K., Shitan, N., Sugiyama, A., Kamimoto, Y., Hamamoto, M., Iwaki, T., Takegawa, K. and Yazaki, K.

    Bioscience, Biotechnology, and Biochemistry   79   2008.2

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  • A double filtering method for measuring the translational velocity of fluorescently stained cells Reviewed International journal

    Yasokawa, T., Ishimaru, I., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   91   2007.12

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  • Fission yeast Sst4p, a conserved Vps27/Hrs homolog, functions downstream of PtdIns 3-kinase Pik3p to mediate proper spore formation. Reviewed International journal

    Onishi, M., Iida, M., Koga, T., Yamada, S., Hirata, A., Iwaki, T., Takegawa, K., Fukui, Y., and Tachikawa, H.

    Eukaryot. Cell   2007.12

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  • Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe. Reviewed International journal

    Iwaki, T., Onishi, M., Ikeuchi, M., Kita, A., Sugiura, R., Giga-Hama, Y., Fukui, Y., and Takegawa, K.

    Microbiology   2007.11

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  • A double filtering method for measuring the translational velocity of fluorescently stained cells Reviewed

    Yasokawa T., Ishimaru I., Kuriyama S., Masaki T., Takegawa K., Tanaka N.

    Applied Physics Letters   91 ( 13 )   2007.10   ISSN:00036951

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    The authors propose a double filtering method to measure translational velocity for tracking fluorescently stained cells. This method employs two diffraction gratings installed in the infinity space through which the parallel pencil beam of the fluorescence passes. With this method, the change in light intensity whose period is proportional to the translational velocity of the sample can be obtained at the imaging surface. By using a sample that has a random distribution of fluorescence intensity, the authors verified that translational velocity measurements could be achieved using the proposed method. © 2007 American Institute of Physics.

    DOI: 10.1063/1.2793695

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  • Identification of the catalytic acid-base residue of Arthrobacter endo-β-N-acetylglucosaminidase by chemical rescue of inactive mutant. Reviewed

    142   2007.10

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  • Identification of the catalytic acid-base residue of arthrobacter endo-β-N-acetylglucosaminidase by chemical rescue of an inactive mutant Reviewed

    Fujita K., Sato R., Toma K., Kitahara K., Suganuma T., Yamamoto K., Takegawa K.

    Journal of Biochemistry   142 ( 3 )   301 - 306   2007.9   ISSN:0021924X

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    Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A), a member of glycoside hydrolase (GH) family 85, catalyses the hydrolysis and transglycosylation of asparagine-linked oligosaccharides of glycoproteins with retention of anomeric configuration. Glu-173 of Endo-A is a catalytically essential amino acid residue, and the corresponding residue is conserved in all GH family 85 members. The catalytic activity of Endo-A E173A mutant was rescued by the addition of sodium azide or sodium formate. Furthermore, the produced β-glycosyl azide (Man 5GlcNAc-β-N 3) retained the anomeric configuration, indicating that Glu-173 is the catalytic acid-base residue of Endo-A. This is the first identification of the catalytic residue for GH family 85 endo-β-N-acetylglucosaminidases. © 2007 The Japanese Biochemical Society.

    DOI: 10.1093/jb/mvm124

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  • A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting Mach-Zehnder interferometer. Reviewed

    Yasokawa, T., Ishimaru, I., Kondo, M., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N.

    Opt. Rev.   14   2007.8

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  • Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe Reviewed

    Iwaki T., Onishi M., Ikeuchi M., Kita A., Sugiura R., Giga-Hama Y., Fukui Y., Takegawa K.

    Microbiology   153 ( 8 )   2753 - 2764   2007.8   ISSN:13500872

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    The multivesicular body (MVB) sorting pathway is required for a number of biological processes, including downregulation of cell-surface proteins and protein sorting into the vacuolar lumen. The function of this pathway requires endosomal sorting complexes required for transport (ESCRT) composed of class E vacuolar protein sorting (Vps) proteins in Saccharomyces cerevisiae, many of which are conserved in Schizosaccharomyces pombe. Of these, sst4/vps27 (homologous to VPS27) and sst6 (similar to VPS23) have been identified as suppressors of sterility in ste12Δ (sst), although their functions have not been uncovered to date. In this report, these two sst genes are shown to be required for vacuolar sorting of carboxypeptidase Y (CPY) and an MVB marker, the ubiquitin-GFP-carboxypeptidase S (Ub-GFP-CPS) fusion protein, despite the lack of the ubiquitin E2 variant domain in Sst6p. Disruption mutants of a variety of other class E vps homologues also had defects in sorting of CPY and Ub-GFP-CPS. Sch. pombe has a mammalian AMSH homologue, sst2. Phenotypic analyses suggested that Sst2p is a class E Vps protein. Taken together, these results suggest that sorting into multivesicular bodies is dependent on class E Vps proteins, including Sst2p, in Sch. pombe. © 2007 SGM.

    DOI: 10.1099/mic.0.2007/006072-0

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  • Technique for measuring the rotational velocity of a single cell. Reviewed International journal

    Yasokawa, T., Ishizaki, T., K. Ishizaki, K. Gesho, Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   90   2007.7

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  • A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting mach-zehnder interferometer Reviewed

    Yasokawa T., Ishimaru I., Kondo M., Kuriyama S., Masaki T., Takegawa K., Tanaka N.

    Optical Review   14 ( 4 )   161 - 164   2007.7   ISSN:13406000

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    This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach-Zehnder interferometer in conjunction with proximal two-beam optical tweezers. © 2007 The Optical Society of Japan.

    DOI: 10.1007/s10043-007-0161-7

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  • Valpronic acid affects trafficking and cell wall integrity in fission yeast. Reviewed International journal

    Miyatake, M., Kuno, T., Kita, A., Katsura, K., Takegawa, K., Nabata, T., and Sugiura, R.

    Genetics   2007.5

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  • Loss of a GPI-anchored membrane protein Aah3p causes a defect of vacuolar protein sorting in Schizosaccharomyces pombe. Reviewed

    Iwaki, T., Morita, T., Tanaka, N., Giga-Hama, Y., and Takegawa, K.

    Biosci. Biotechnol. Biochem.   71   2007.4

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  • Loss of a GPI-anchored membrane protein Aah3p causes a defect in vacuolar protein sorting in Schizosaccharomyces pombe Reviewed

    Iwaki T., Morita T., Tanaka N., Giga-Hama Y., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   71 ( 2 )   623 - 626   2007.3   ISSN:09168451

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    Schizosaccharomyces pombe has four α-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Δ was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Δ, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Δ had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting.

    DOI: 10.1271/bbb.60609

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  • Method for measuring the three dimensional distribution of a fluorescent dye in a cell membrane. Reviewed International journal

    Yamamoto, K., Ishimaru, I., Fujii, Y., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   90   2007.2

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  • New amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast. Reviewed International journal

    Ma, Y., Sugiura, R., Saito, M., Koike, A., Sio, S.-O., Fujita, Y., Takegawa, K. and Kuno, T.

    Curr.Genet.   2007.2

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  • Minimum genome factory using fission yeast Schizosaccharomyces pombe, improvement of host genome for heterologous protein production. Reviewed International journal

    Giga-Hama, Y., Tohda, H., Takegawa, K., and Kumagai, H.

    Biotechnol. Appl. Biochem.   46   2007.2

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  • Displacement measurement of the depth migration of transparent cells Reviewed

    Yoshida M., Ishimaru I., Ishizaki K., Yasokawa T., Kuriyama S., Masaki T., Nakai S., Takegawa K., Tanaka N.

    Applied Physics Letters   89 ( 24 )   2006.12   ISSN:00036951

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    This letter reports a method for displacement measurement of the depth migration of transparent cells. This proposed optical spatial filtering method allows visualization of the transparent cells and determination of depth migration as a horizontal displacement positive or negative first order diffracted light on the detector surface. When the sample is displaced upward or downward from the focal plane, first and negative first order diffracted light form images at a different point as a light circle. The coordinates of these two light circles on the detector surface change places when the displacement of depth migration moves to the opposite direction. © 2006 American Institute of Physics.

    DOI: 10.1063/1.2405396

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  • Three dimentional phase-contrast Imaging for single floating cells. Reviewed International journal

    Kobayashi, H., Ishimaru, I., Yasokawa, T., Ishizaki, K., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   89   2006.12

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  • Imaging technology of three dimensional distribution for sugar chain on single living cell-membrane Reviewed

    Yamamoto K., Ishimaru I., Fujii Y., Yasokawa T., Ishizaki K., Yoshida M., Takegawa K., Tanaka N., Kuriyama S., Masaki T., Nakai S.

    Proceedings of SPIE - The International Society for Optical Engineering   6374   2006.12   ISSN:0277786X ISBN:0819464724, 9780819464729

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    We study on the imaging technology of three-dimensional distribution for sugar chain on single living cell-membrane. This technology can observe the entire cell surface. To observe the cell surface, the local area image of cell-membrane is taken by TIRF (total internal reflection fluorescence) microscopy. And by scanning the whole cell surface area, we can obtain the image of the entire cell membrane. These observed local area images can be converted into an entire surface image by the pattern matching processing. For this scanning technology, we propose the proximal two beam optical tweezers to rotate the single floating cell. This proximal two beam optical tweezers can rotate the floating single cell in the nutrient medium by light pressure. Two beams illuminate the single cell at proximal two points from below and above. The cell is trapped at the center of these two focal points. At the same time, light pressures that are generated at two focal points are made to act as rotational torque. Conventionally TIRF microscope is well known as the observation technology for the cell-membrane using the evanescent light as the exciting light. We can observe the local area images of the fluorescently labeled sugar chain that binds the glycoprotein. Using the proposed optical system, we can obtain the fluorescent distribution images on the cell-membrane.

    DOI: 10.1117/12.685893

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  • A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant. Reviewed International journal

    Iwaki, T., Giga-Hama, Y., and Takegawa, K.

    Microbiology   2006.11

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  • Displacement measurement of the depth migration of transparent cells. Reviewed International journal

    Yoshida, M., Ishimaru, I., Ishizaki, K., Yasokawa, T., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   89   2006.11

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  • Attitudinal manipulation of an optically trapped bacillary probe by controlling the distance between focal points for local dosing in cells Reviewed

    Yasokawa T., Ishimaru I., Nakagawa Y., Kuriyama S., Masaki T., Nakai S., Takegawa K., Tanaka N.

    Applied Physics Letters   89 ( 13 )   2006.10   ISSN:00036951

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    Technology for the attitudinal manipulation of medical equipment for local dosing to single living cells is presented. The authors developed a "juggling probe" which can manipulate a bacillary probe with multiple degrees of freedom and high accuracy without physical contact by using light pressure. With this technology, the authors cause two beams to focus on the probe from both the top and bottom faces. When the two focal points coincide, the probe becomes trapped immediately in a stable fixed attitude. Furthermore, the probe remains at rest in an arbitrary tilted attitude simply by controlling the distance between the two focal points. © 2006 American Institute of Physics.

    DOI: 10.1063/1.2357922

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  • Variable phase-contrast fluorescence spectrometry for fluorescently stained cells. Reviewed International journal

    Inoue, Y., Ishimaru, I., Yasokawa, T., Ishizaki, K., Yoshida, M., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   2006.10

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  • Attitudinal manipulation of an optically trapped bacillary probe by controlling the distance between focal points for local dosing in cells. Reviewed International journal

    Yasokawa, T., Ishimaru, I., Nakagawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   89   2006.10

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  • Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe Reviewed

    Fujita Y., Tohda H., Giga-Hama Y., Takegawa K.

    FEMS Yeast Research   6 ( 6 )   883 - 887   2006.9   ISSN:15671356

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    A new, heat shock-inducible expression system based on an endogenous hsp16+ promoter was developed for use in the fission yeast Schizosaccharomyces pombe. Analysis of GFP expression profiles indicated that a 1.2-kb segment of the hsp16+ promoter region was sufficient to drive expression of heterologous protein. The hsp16+ promoter was found to be activated not only by heat shock but also by other stresses including cadmium, ethanol, and oxidative stress. Two expression vectors, pHIL and pHIU, were constructed using the 1.2-kb hsp16+ promoter for inducible gene expression in Sch. pombe. This new expression system utilizes a simple induction protocol and promises to be a useful tool for analyzing gene expression in Sch. pombe. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.

    DOI: 10.1111/j.1567-1364.2006.00093.x

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  • A survey of all 11 ABC transporters in fission yeast: Two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant Reviewed

    Iwaki T., Giga-Hama Y., Takegawa K.

    Microbiology   152 ( 8 )   2309 - 2321   2006.8   ISSN:13500872

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    ATP-binding cassette (ABC) proteins transport a wide variety of substrates, including sugars, amino acids, metal ions, lipids, peptides and proteins, across membranes, and most ABC proteins contain transmembrane domains (ABC transporters). Sequencing of the Schizosaccharomyces pombe genome has allowed identification of all genes encoding ABC transporters in fission yeast. To date, six such genes have been characterized, and an additional five genes encoding ABC transporters were identified from the genome sequence. In an attempt to characterize all of the ABC transporters in fission yeast, all 11 genes were disrupted. While all the genes were found to be dispensable for cell viability, some disruptants lacked apparent phenotypes. GFP-tagged ABC transporters were localized to membranes as follows: plasma membrane (2), vacuolar membrane (4), mitochondrial membrane (2), endoplasmic reticulum membrane (2), and endosome and Golgi membranes (1). Two Cluster II. 1 proteins, Abc2p (SPAC3F10.11c) and Abc4p (SPAC30.04c), were found to be localized to vacuolar membranes, and to be responsible for accumulation of a characteristic red pigment in the vacuole of an adenine biosynthetic mutant. The doubly disrupted mutant abc2Δ abc4Δ exhibited drug sensitivity, and a decreased accumulation of monochlorobimane, suggesting that both of the proteins encoded by these genes are involved in detoxification of xenobiotics, and vacuolar sequestration of glutathione S-conjugates. © 2006 SGM.

    DOI: 10.1099/mic.0.28952-0

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  • An α-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe Reviewed

    Morita T., Tanaka N., Hosomi A., Giga-Hama Y., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   70 ( 6 )   1454 - 1463   2006.7   ISSN:09168451

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    Glycosylphosphatidylinositol (GPI)-anchored proteins are essential for normal cellular morphogenesis and have an additional role in mediating cross-linking of glycoproteins to cell wall glucan in yeast cells. Although many GPI-anchored proteins have been characterized in Saccharomyces cerevisiae, none have been reported for well-characterized GPI-anchored proteins in Schizosaccharomyces pombe to date. Among the putative GPIanchored proteins in S. pombe, four α-amylase homologs (Aah1p-Aah4p) have putative signal sequences and C-terminal GPI anchor addition signals. Disruption of aah3 + resulted in a morphological defect and hypersensitivity to cell wall-degrading enzymes. Biochemical analysis showed that Aah3p is an N-glycosylated, GPI-anchored membrane protein localized in the membrane and cell wall fractions. Conjugation and sporulation were not affected by the aah3 + deletion, but the ascal wall of aah3Δ cells was easily lysed by hydrolases. Expression of aah3 alleles in which the conserved aspartic acid and glutamic acid residues required for hydrolase activity were replaced with alanine residues failed to rescue the morphological and ascal wall defects of aah3Δ cells. Taken together, these results indicate that Aah3p is a GPI-anchored protein and is required for cell and ascal wall integrity in S. pombe.

    DOI: 10.1271/bbb.50693

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  • Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. Reviewed International journal

    Fujita, Y., Tohda, H., Giga-Hama, Y., and Takegawa, K.

    FEMS Yeast Res.   2006.7

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  • A pricise method for rotating single cells. Reviewed International journal

    Kobayashi, H., Ishimaru, I., Hyodo, R., Yasokawa, T., Ishizaki, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   88   2006.7

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  • Identification of the catalytic acid-base residue of Arthrobacter endo-β-N-acetylglucosaminidase by chemical rescue of inactive mutant. Reviewed International journal

    2006.7

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  • Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Reviewed International journal

    Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y., Tanaka, N., and Takegawa, K.

    Microbiology   2006.7

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  • Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus. Reviewed International journal

    Mai, H.T., Nomura, M., Takegawa, K., Asamizu E., Sato S., Kato T., Tabata S., and Tajima, S.

    Plant Cell Physiol.   2006.7

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  • Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus Reviewed

    Mai H.T., Nomura M., Takegawa K., Asamizu E., Sato S., Kato T., Tabata S., Tajima S.

    Plant and Cell Physiology   47 ( 7 )   829 - 838   2006.7   ISSN:00320781

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    We identified a Sed5-like clone LjSYP32-1 which contributes to nodule tissue formation and plant growth in Lotus japonicus. In the L. japonicus expressed sequence tag (EST) clone databases of Kazusa DNA Research Institute, another syntaxin-related clone (LjSYP32-2) was also detected, and the nucleotide and amino acid sequences of these two clone are very similar to each other. Real-time PCR and promoter analysis indicated that expression of LjSYP32-1 was dominant compared with LjSYP32-2 in the various plant organs. Promoter analysis and in situ hybridization revealed that LjSYP32-1 was expressed significantly in the inner cortex cell layer surrounding the infected zone of young nodules and in the meristem area of developing lateral root. To explore the function and physiological role of LjSYP32-1 in nodules and other plant organs, stable transformation lines of L. japonicus expressing either sense or antisense LjSYP32-1 were prepared. The antisense plants showed a significantly retarded plant growth phenotype, suggesting a role for LjSYP32-1 in supporting plant growth. In the same transgenic lines, the plants were capable of forming nodules, but the acetylene reduction activity was reduced by around 50% per plant. The nodules were much smaller and some nodules were fused to each other by sharing the inner cortex. The rate of occurence of such irregular nodules was twice that observed in wild-type plants. The data suggest that LjSYP32-1 contributes to the support of plant growth and normal nodule tissue differentiation. © The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.

    DOI: 10.1093/pcp/pcj054

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  • Translational velocity measurement for single floating cell based on optical Fourier transform theory. Reviewed International journal

    Ishizaki, K., Ishimaru, I., Yoshida, M., Inoue, Y., Yasokawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N.

    Appl. Phys. Lett.   88   2006.6

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  • A precise method for rotating single cells Reviewed

    Kobayashi H., Ishimaru I., Hyodo R., Yasokawa T., Ishizaki K., Kuriyama S., Masaki T., Nakai S., Takegawa K., Tanaka N.

    Applied Physics Letters   88 ( 13 )   2006.4   ISSN:00036951

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    A precise method to rotate single cells is reported. In this method, the light pressure in the optical axis direction is harnessed as a rotating torque. Two proximal points in each cell are illuminated from different directions using two beams, and a light pressure is created that acts as a rotating torque. Using this proposed method, we could control the rotational direction of a microsphere regardless of the refractive index distribution in a noncontact operation. The microsphere could be rotated using proximal two-beam optical tweezers, and the rotational velocity could be controlled by changing the light intensity. © 2006 American Institute of Physics.

    DOI: 10.1063/1.2190268

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  • Genetic and functional interaction between Ryh1 and Ypt3: Two Rab GTPases that function in S. pombe secretory pathway Reviewed

    He Y., Sugiura R., Ma Y., Kita A., Deng L., Takegawa K., Matsuoka K., Shuntoh H., Kuno T.

    Genes to Cells   11 ( 3 )   207 - 221   2006.3   ISSN:13569597

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    We have previously isolated ypt3-i5 mutant and showed that Ypt3 GTPase functions in the fission yeast secretory pathway. Here, the same genetic screen led to the isolation of ryh1-i6, a mutant allele of the ryh1+ gene encoding a homolog of Rab6. The ryh1-i6 mutant showed phenotypes that support its role in retrograde traffic from endosome to the Golgi. Interestingly, ryh1+ gene deletion was synthetically lethal with ypt3-i5 mutation. Consistently, the over-expression of the GDP-conformational mutant, Ryh1T25 N, inhibited the growth of ypt3-i5 mutant but had no effect on that of wild-type cells. Furthermore, the over-expression of the Ryh1T25N mutant inhibited the acid phosphatase glycosylation and exacerbated the cell wall integrity of ypt3-i5 mutant, but had no effect on those of wild-type cells. GFP-Ryh1 and GFP-Ypt3 both localized at the Golgi/endosome, but showed distinct subcellular localizations. The localization of GFP-Ryh1 in ypt3-i5 mutant and that of GFP-Ypt3 in ryh1-i6 mutant were distinct from those in wild-type cells. In addition, Ryh1 as well as Ypt3 were shown to be involved in acid phosphatase secretion. These results suggest that Ryh1 is involved in the secretory pathway and may have a potential overlapping function with Ypt3 in addition to its role in recycling. © 2006 The Author(s) Journal compilation © 2006 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd.

    DOI: 10.1111/j.1365-2443.2006.00935.x

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  • Chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures Reviewed

    Haneda K., Takeuchi M., Tagashira M., Inazu T., Toma K., Isogai Y., Hori M., Kobayashi K., Takeuchi M., Takegawa K., Yamamoto K.

    Carbohydrate Research   341 ( 2 )   181 - 190   2006.2   ISSN:00086215

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    Naturally occurring glycopeptides and glycoproteins usually contain more than one glycosylation site, and the structure of the carbohydrate attached is often different from site to site. Therefore, synthetic methods for preparing peptides and proteins that are glycosylated at multiple sites, possibly with different carbohydrate structures, are needed. Here, we report a chemo-enzymatic approach for accomplishing this. Complex-type oligosaccharides were introduced to the calcitonin derivatives that contained two N-acetyl-d-glucosamine (GlcNAc) residues at different sites by treatment with Mucor hiemalis endo-β-N-acetylglucosaminidase. Using this enzymatic transglycosylation reaction, three glycopeptides were produced, a calcitonin derivative with the same complex-type carbohydrate at two sites, and two calcitonin derivatives each with one complex-type carbohydrate and one GlcNAc. Starting from the derivatives with one complex-type carbohydrate and one GlcNAc, a high-mannose-type oligosaccharide was successfully transferred to the remaining GlcNAc using another endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae. Thus, we were able to obtain glycopeptides containing not only two complex-type carbohydrates, but also both complex and high-mannose-type oligosaccharides in a single molecule. Using the resultant glycosylated calcitonin derivatives, the effects of di-N-glycosylation on the structure and the activity of calcitonin were studied. The effect appeared to be predictable from the results of mono-N-glycosylated calcitonin derivatives. © 2005 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.carres.2005.11.015

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  • Homocysteine accumulation causes a defect in purine biosynthesis: further characterization of Schizosaccharomyces pombe methionine auxotrophs. Reviewed International journal

    Fujita, Y., Ukena, E., Iefuji, H., Giga-Hama, Y., and Takegawa, K.

    Microbiology   2006.2

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  • Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway. Reviewed International journal

    He, Y., Sugiura, R., Ma, Y., Kita, A., Deng, L., Takegawa, K., Matsuoka, K., Shuntoh, H., and Kuno, T.

    Genes Cells   2006.2

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  • A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast Reviewed

    Hirashima K., Iwaki T., Takegawa K., Giga-Hama Y., Tohda H.

    Nucleic Acids Research   34 ( 2 )   1 - 7   2006.2   ISSN:03051048

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    The technologies for chromosome modification developed to date are not satisfactorily universal, owing to the typical requirements for special enzymes and sequences. In the present report, we propose a new approach for chromosome modification in Schizosaccharomyces pombe that does not involve any special enzymes or sequences. This method, designated the 'Latour system', has wide applicability with extremely high efficiency, although both the basic principle and the operation are very simple. We demonstrate the ability of the Latour system to discriminate essential genes, with a long chromosomal area of 100 kb containing 33 genes deleted simultaneously and efficiently. Since no foreign sequences are retained after deletion using the Latour system, this system can be repeatedly applied at other sites. Provided that a negative selectable marker is available, the Latour system relies solely upon homologous recombination, which is highly conserved in living organisms. For this reason, it is expected that the system will be applicable to various yeasts. © The Author 2006. Published by Oxford University Press. All rights reserved.

    DOI: 10.1093/nar/gnj011

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  • Homocysteine accumulation causes a defect in purine biosynthesis: Further characterization of Schizosaccharomyces pombe methionine auxotrophs Reviewed

    Fujita Y., Ukena E., Iefuji H., Giga-Hama Y., Takegawa K.

    Microbiology   152 ( 2 )   397 - 404   2006.2   ISSN:13500872

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    Methionine synthase (EC2.1.1.14) catalyses the final step in methionine synthesis, i.e. methylation of homocysteine. A search of the Schizosaccharomyces pombe genomic database revealed a gene designated SPAC9.09, encoding a protein with significant homology to methionine synthase. Disruption of SPAC9.09 caused methionine auxotrophy, and thus the gene was identified as a methionine synthase and designated met26. The met26 mutant was found to exhibit a remarkable growth defect in the absence of adenine even in medium supplemented with methionine. This phenotype was not observed in other methionine auxotrophs. In the budding yeast Saccharomyces cerevisiae, which has been reported to utilize homocysteine in cysteine synthesis, lack of a functional methionine synthase did not cause a requirement for adenine. The introduction of genes from Sac. cerevisiae constituting the cystathionine pathway (CYS4 and CYS3) into Sch. pombe Δmet26 cells restored growth in the absence of adenine. HPLC analysis showed that total homocysteine content in Δmet26 cells was higher than in other methionine auxotrophs and that introduction of the Sac. cerevisiae cystathionine pathway decreased total homocysteine levels. These data demonstrate that accumulation of homocysteine causes a defect in purine biosynthesis in the met26 mutant. © 2006 SGM.

    DOI: 10.1099/mic.0.28398-0

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  • Chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures. Reviewed International journal

    Haneda, K., Takeuchi, M., Tagashira, M., Inazu, T., Toma, K., Isogai, Y., Hori, M., Kobayashi, K., Takeuchi, M., Takegawa, K., and Yamamoto, K.

    Carbohydr. Res   2006.1

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  • A role for fission yeast rab GTPase Ypt7p in sporulation Reviewed

    Kashiwazaki J., Nakamura T., Iwaki T., Takegawa K., Shimoda C.

    Cell Structure and Function   30 ( 2 )   43 - 49   2005.12   ISSN:03867196

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    Ypt7p, a fission yeast (Schizosaccharomyces pombe) homologue of Rab7 GTPase, mediates fusion of endosomes to vacuoles and homotypic vacuole fusion. Here, we report that Ypt7p plays important roles in sporulation. Most ypt7Δ asci produced less than four spores, which were apparently immature and germinated at low frequency. Furthermore, ypt7Δ cells were defective in development of the forespore membranes. Vacuoles in sporulating cells were found to undergo extensive homotypic vacuole fusion to form a few large compartments occupying the entire cytoplasm of asci. This extensive vacuole fusion depended on Ypt7p. © 2005 by Japan Society for Cell Biology.

    DOI: 10.1247/csf.30.43

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  • Mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe Reviewed

    Iwaki T., Fujita Y., Tanaka N., Giga-Hama Y., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   69 ( 11 )   2109 - 2116   2005.12   ISSN:09168451

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    A potential correlation between mitochondrial and vacuolar functions is known to exit in yeast. Fission yeast atm1+, SPAC15A10.01, encodes a putative half-type ABC transporter with an N-terminal mitochondrial-targeting signal. In an attempt to evaluate the possible involvement of mitochondrion in vacuole function, a functional analysis of atm1+ was performed by gene disruption. Growth of the atm1 mutant was inhibited in the presence of oxidizing agents, and S. cerevisiae Atm1p was found to complement this growth defect. atm1Δ cells exhibited defects in fluid-phase endocytosis and vacuolar fusion under hypotonic stress. GFP-tagged Atm1p was observed to be localized in the mitochondria. These data strongly suggest that fission yeast Atm1p was not only involved in protection against oxidative stress, but also played a role in vacuolar functions.

    DOI: 10.1271/bbb.69.2109

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  • Proximal two-beam optical tweezers for rotational control of living single-cells Reviewed

    Yoshida M., Ishimaru I., Ishizaki K., Yasokawa T., Kuriyama S., Masaki T., Nakai S., Takegawa K., Tanaka N.

    Proceedings of the SICE Annual Conference   3470 - 3474   2005.12

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    Currently, we study about spectroscopy-tomography of living single-cells whose diameter is about 10 μm to assist the quantitative diagnosis of early cancer. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single-cell rotating method. In these two technologies, we propose proximal two-beam optical tweezers as the precise rotating method. In this method, we harness the light pressure generated by light absorption as rotating torque. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can control the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell. © 2005 SICE.

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  • A mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe. Reviewed

    Iwaki T, Fujita Y, Tanaka N, Giga-Hama Y and Takegawa K

    Bioscience Biotechnology and Biochemistry   2005.10

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    DOI: 10.1271/bbb.69.2109.

  • Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants Reviewed

    Goldar M.M., Nishie T., Ishikura Y., Fukuda T., Takegawa K., Kawamukai M.

    Bioscience, Biotechnology and Biochemistry   69 ( 7 )   1422 - 1426   2005.9   ISSN:09168451

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    The moc1/sds23 gene was isolated to induce sexual development of a sterile strain due to overexpression of adenylate cyclase in Schizosaccharomyces pombe. Here, we studied the functional conservation between moc1/ sds23 and its two orthologs SDS23 and SDS24 in Saccharomyces cerevisiae. We observed that the temperature sensitivity, salt tolerance, cell morphology, and sterility of the Δmoc1 mutant in S. pombe were recovered by expressing either S. cerevisiae SDS23 or SDS24. We found that deletion of both SDS23 and SDS24 resulted in the production of a large vacuole that was reversed by the expression of S. pombe moc1/sds23. In these ways we found that S. pombe Moc1/Sds23 and S. cerevisiae SDS23p or SDS24p are functional homologs. In addition we found that the Δsds23 Δsds24 diploid strain reduces cell separation in forming pseudohyphal-like growth in S. cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar.

    DOI: 10.1271/bbb.69.1422

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  • Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants. Reviewed

    Goldar MM, Nishie T, Ishikura Y, Fukuda T, Takegawa K and Kawamukai M

    Bioscience, Biotechnology and Biochemistry   2005.8

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    DOI: 10.1271/bbb.69.1422.

  • Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe Reviewed

    Tanaka N., Fujita Y., Suzuki S., Morishita M., Giga-Hama Y., Shimoda C., Takegawa K.

    Biochemical and Biophysical Research Communications   330 ( 3 )   813 - 820   2005.5   ISSN:0006291X

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    Protein O-glycosylation is an essential protein modification in eukaryotic cells. In Saccharomyces cerevisiae, O-mannosylation is initiated in the lumen of the endoplasmic reticulum by O-mannosyltransferase gene products (Pmt1p-7p). A search of the Schizosaccharomyces pombe genome database revealed a total of three O-glycoside mannosyltransferase homologs (ogm1+, ogm2 +, and ogm4+), closely related to Saccharomyces cerevisiae PMT1, PMT2, and PMT4. Although individual ogm genes were not found to be essential, ogm1Δ and ogm4Δ mutants exhibited aberrant morphology and failed to agglutinate during mating. The phenotypes of the ogm4Δ mutant were not complemented by overexpression of ogm1+ or ogm2+, suggesting that each of the Ogm proteins does not have overlapping functions. Heterologous expression of a chitinase from S. cerevisiae in the ogm mutants revealed that O-glycosylation of chitinase had decreased in ogm1Δ cells. A GFP-tagged Fus1p from S. cerevisiae was specifically not glycosylated and accumulated in the Golgi in ogm4Δ cells. These results indicate that O-glycosylation initiated by Ogm proteins plays crucial physiological roles and can serve as a sorting determinant for protein transport of membrane glycoproteins in S. pombe. © 2005 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2005.03.033

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  • Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe. Reviewed International journal

    Tanaka N, Fujita Y, Suzuki S, Morishita M, Giga-Hama Y, Shimoda C and Takegawa K

    Biochemical and Biophysical Research Communiactions   2005.5

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    DOI: 10.1016/j.bbrc.2005.03.033.

  • An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus Reviewed

    Kimura Y., Ohtani M., Takegawa K.

    Journal of Bacteriology   187 ( 10 )   3593 - 3598   2005.5   ISSN:00219193

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    We have previously reported that a receptor-type adenylyl cyclase (CyaA) of Myxococcus xanthus undergoes an osmosensor mainly during spore germination (Y. Kimura et al., J. Bacteriol. 184:3578-3585, 2002). In the present study, we cloned another receptor-type adenylyl cyclase gene (cyaB) and characterized the function of the cyaB-encoded protein. Disruption of cyaB generates a mutant that showed growth retardation at high ionic (NaCl) or high nonionic (sucrose) osmolarity. When vegetative cells were stimulated with 0.15 M NaCl, the increases in intracellular cyclic AMP levels of cyaB mutant cells were lower than those of wild-type cells. Under nonionic osmostress, the cyaB mutant exhibited reduced spore germination; however, the germination rate of the cyaB mutant was significantly higher than that of the cyaA mutant. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

    DOI: 10.1128/JB.187.10.3593-3598.2005

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  • An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus. Reviewed International journal

    Kimura Y, Ohtani M and Takegawa K

    Journal of Bacteriology   2005.4

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    DOI: 10.1128/JB.187.10.3593-3598.2005.

  • Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe. Reviewed International journal

    Fujita Y, Giga-Hama Y and Takegawa K

    Yeast   2005.2

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    DOI: doi: 10.1002/yea.1201.

  • Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe Reviewed

    Fujita Y., Giga-Hama Y., Takegawa K.

    Yeast   22 ( 3 )   193 - 202   2005.2   ISSN:0749503X

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    We describe the development of a new transformation system, using multiple auxotrophic marker genes, for the fission yeast Schizosaccharomyces pombe. We developed three new auxotrophic marker genes (arg12+, tyr1+ and ade7+) and generated a new host strain, YF043, by Cre-loxP-mediated gene disruption. YF043 possessed six mutated biosynthetic genes (leu1-32, ura4-M190T, arg12::loxP, tyr1::loxP, ade7::loxP and his2::loxP). The combination of this host strain and the new selectable markers can be used for gene disruption using the same preexisting transformation systems. In addition, Sz. pombe vectors were constructed, containing selectable marker genes that complement the auxotrophies of YF043. These new vectors are available for gene disruption and heterologous protein expression in strain YF043. The new Sz. pombe host strain will be a useful tool for molecular genetic studies of Sz. pombe where multiple recombinant modifications or multiple mutations are needed. Copyright © 2005 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.1201

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  • A role for fission yeast Rab GTPase Ypt7p in sporulation. Reviewed International journal

    Kashiwazaki J, Nakamura T, Iwaki T, Takegawa K and Shimoda C

    Cell Structure and Function   2005.1

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    DOI: doi: 10.1247/csf.30.43.

  • Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe. Reviewed

    Koga T, Onishi M, Nakamura Y, Hirata A, Nakamura T, Shimoda C, Iwaki T, Takegawa K and Fukui Y

    Genes to Cells   2004.9

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    DOI: 10.1111/j.1356-9597.2004.00744.x.

  • Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe. Reviewed International journal

    Iwaki T, Tanaka N, Takagi H, Giga-Hama Y and Takegawa K

    Yeast   2004.9

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    DOI: 10.1002/yea.1134.

  • Loss of Apm1, the AP-1 clathrin-adaptor gamma1 subunit, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast. Reviewed International journal

    Kita A, Sugiura R, He Y, Deng L, Lu Y, Sio SO, Takegawa K, Sakaue M, Shuntoh H and Kuno T

    Molecular Biology of the Cell   2004.8

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    DOI: 10.1091/mbc.e03-09-0659.

  • Characterization of end4<sup>+</sup>, a gene required for endocytosis in Schizosaccharomyces pombe Reviewed

    Iwaki T., Tanaka N., Takagi H., Giga-Hama Y., Takegawa K.

    Yeast   21 ( 10 )   867 - 881   2004.7   ISSN:0749503X

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    To understand endocytic trafficking in Schizosaccharomyces pombe, we constructed an end4 disruption mutant. The end4+ gene encodes a protein homologous to Sla2p/End4p, which is essential for the assembly and function of the cytoskeleton and endocytosis in Saccharomyces cerevisiae. We characterized the fission yeast mutant end4Δ as well as ypt7Δ, which is deficient in vacuolar fusion and, hence, endocytosis. The delivery of FM4-64 to the vacuolar membrane, accumulation of Lucifer yellow CH and internalization of plasma membrane protein Map3-GFP were inhibited in the end4 mutant. Deletion of end4 resulted in pleiotropic phenotypes consistent with F-actin depolarization, including high temperature sensitivity, abnormal morphology and mating defects. Extensive missorting of carboxypeptidase Y was detected in the ypt7 mutant; however, little missorting was detected in the end4 mutant. These results indicate that End4p is essential for the internalization process and Ypt7p affects endocytosis at a post-internalization step after the intersection of the endocytic and the vacuolar protein-sorting pathways in fission yeast. Copyright © 2004 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.1134

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  • Characterization of endo--N-acetylglucosaminidase from alkaliphilic Bacillus halodurans C-125. Reviewed

    2004.7

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    DOI: 10.1271/bbb.68.1059.

  • Characterization of Schizosaccharomyces pombe mutants defective in vacuolar acidification and protein sorting. Reviewed International journal

    Iwaki T, Goa T, Tanaka N and Takegawa K

    Molecular Genetics and Genomics   2004.6

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    DOI: 10.1007/s00438-003-0971-7.

  • A simple and efficient procedure for transformation of Schizosaccharomyces pombe. Reviewed International journal

    Morita T and Takegawa K

    Yeast   2004.6

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    DOI: 10.1002/yea.1104.

  • A simple and efficient procedure for transformation of Schizosaccharomyces pombe Reviewed

    Morita T., Takegawa K.

    Yeast   21 ( 8 )   613 - 617   2004.6   ISSN:0749503X

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    We describe a simple and efficient procedure for transformation of Schizosaccharomyces pombe. Sz. pombe colonies grown on minimal (SD) plates were directly removed and suspended in a 100 μl reaction mixture containing 70 μl PLATE solution (50% polyethylene glycol-4000, 100 mM lithium acetate, 10 mM Tris-HCl, pH 4.9, and 1 mM EDTA), 10 μl plasmid DNA (1 μg), 10 μl carrier DNA (100 μg) and 10 μl sterile distilled water. After incubation at 30°C for 1 h followed by heat shock treatment at 42°C for 15 min, the reaction mixture was spread on a selection plate. The transformation efficiency obtained using the procedure was approximately 8000 transformants/μg DNA. The method is simple and time-saving, making it especially useful for a large number of samples and when a high transformation efficiency is not required. Copyright © 2004 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.1104

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  • Loss of Apm1, the μ1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast Reviewed

    Kita A., Sugiura R., Shoji H., He Y., Deng L., Lu Y., Sio S.O., Takegawa K., Sakaue M., Shuntoh H., Kuno T.

    Molecular Biology of the Cell   15 ( 6 )   2920 - 2931   2004.6   ISSN:10591524

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    Calcineurin is a highly conserved regulator of Ca2+ signaling in eukaryotes. In fission yeast, calcineurin is not essential for viability but is required for cytokinesis and Cl- homeostasis. In a genetic screen for mutations that are synthetically lethal with calcineurin deletion, we isolated a mutant, cis1-1/apm1-1, an allele of the apm1+ gene that encodes a homolog of the mammalian μ1A subunit of the clathrin-associated adaptor protein-1 (AP-1) complex. The cis1-1/apm1-1 mutant as well as the apm1-deleted (Δapm1) cells showed distinct phenotypes: temperature sensitivity; tacrolimus (FK506) sensitivity; and pleiotropic defects in cytokinesis, cell integrity, and vacuole fusion. Electron micrographs revealed that Δapm1 cells showed large vesicular structures associated with Golgi stacks and accumulated post-Golgi secretory vesicles. Δapm1 cells also showed the massive accumulation of the exocytic v-SNARE Syb1 in the Golgi/endosomes and a reduced secretion of acid phosphatase. These phenotypes observed in apm1 mutations were accentuated upon temperature up-shift and FK506 treatment. Notably, Apm1-GFP localized to the Golgi/endosomes, the spindle pole bodies, and the medial region. These findings suggest a role for Apm1 associated with the Golgi/endosome function, thereby affecting various cellular processes, including secretion, cytokinesis, vacuole fusion, and cell integrity and also suggest that calcineurin is involved in these events.

    DOI: 10.1091/mbc.E03-09-0659

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  • Disruption of plr1+ gene encoding pyridoxal reductase of Schizosaccharomyces pombe. Reviewed

    Morita T, Takegawa K and Yagi T

    Journal of Biochemistry   2004.5

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    DOI: 10.1093/jb/mvh026.

  • Characterization of endo-β-N-acetylglucosaminidase from alkaliphilic Bacillus halodurans C-125 Reviewed

    Fujita K., Takami H., Yamamoto K., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   68 ( 5 )   1059 - 1066   2004.5   ISSN:09168451

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    The genome sequencing project on alkaliphilic Bacillus halodurans C-125 revealed a putative endo-β-N-acetylglucosaminidase (Endo-BH), which consists of a signal peptide of 24 amino acids, a catalytic region of 634 amino acids exhibiting 50.1% identity with the endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A), and a C-terminal tail of 220 amino acids. Transformed Escherichia coli cells carrying the Endo-BH gene exhibited endo-β-N-acetylglucosaminidase activity. Recombinant Endo-BH hydrolyzed high-mannose type oligosaccharides and hybrid type oligosaccharides, and showed transglycosylation activity. On deletion of 219 C-terminal amino acid residues of Endo-BH, the wild type level of activity was retained, whereas with deletions of the Endo-A homolog domain, the proteins were expressed as inclusion bodies and these activities were reduced. These results suggest that the enzymatic properties of Endo-BH are similar to those of Endo-A, and that the C-terminal tail does not affect the enzyme activity. Although the C-terminal tail region is not essential for enzyme activity, the sequence is also conserved among endo-β-N-acetylglucosaminidases of various origins.

    DOI: 10.1271/bbb.68.1059

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  • A set of LoxP marker cassette for Cre-mediated multiple gene disruption in Schizosaccharomyces pombe. Reviewed

    Iwaki T and Takegawa K

    Bioscience, Biotechnology and Biochemistry   2004.4

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    DOI: 10.1271/bbb.68.545.

  • Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe. Reviewed

    Fujita Y and Takegawa K

    Bioscience, Biotechnology and Biochemistry   2004.3

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    DOI: 10.1271/bbb.68.306.

  • A set of loxP marker cassettes for cre-mediated multiple gene disruption in Schizosaccharomyces pombe Reviewed

    Iwaki T., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   68 ( 3 )   545 - 550   2004.3   ISSN:09168451

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    For functional analysis, the presence of gene families and isoenzymes often makes it necessary to delete more than one gene, while the number of marker genes is limited in Schizosaccharomyces pombe. Here we describe a loxP-flanked ura4+ cassette and Cre recombinase vector for a Cre-loxP-mediated marker removal procedure in S. pombe. This loxP-ura4-loxP cassette can be used for disruption of hmt1+ as a model target gene. We have constructed two vectors which express Cre recombinase under the control of the nmt1 or nmt41 promoter. Excisive recombination at loxP sites in the chromosome was promoted efficiently and accurately when the Cre recombinase was expressed under the control of the nmt41 promoter. In addition, ura4+ could be excised from the genome by Cre recombinase, when a single loxP site was adjacent to ura4. The use of the Cre-loxP system proved to be a practical strategy to excise a marker gene for repeated use in S. pombe.

    DOI: 10.1271/bbb.68.545

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  • Characterization of Schizosaccharomyces pombe mutants defective in vacuolar acidification and protein sorting Reviewed

    Iwaki T., Goa T., Tanaka N., Takegawa K.

    Molecular Genetics and Genomics   271 ( 2 )   197 - 207   2004.3   ISSN:16174615

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    The vacuolar H+ -ATPases (V-ATPases) are ATP-dependent proton pumps responsible for acidification of intracellular compartments in eukaryotic cells. To investigate the functional roles of the V-ATPase in Schizosaccharomyces pombe, the gene vma1 encoding subunit A or vma3 encoding subunit c was disrupted. Both deletion mutants lost the capacity for vacuolar acidification in vivo, and showed sensitivity to neutral pH or high concentrations of divalent cations including Ca2+. The delivery of FM4-64 to the vacuolar membrane and accumulation of Lucifer Yellow CH were strongly inhibited in the vma1 and vma3 mutants. Moreover, deletion of the S. pombe vma1 or vma3+ gene resulted in pleiotropic phenotypes consistent with lack of vacuolar acidification, including the missorting of vacuolar carboxypeptidase Y, abnormal vacuole morphology, and mating defects. These findings suggest that V-ATPase is essential for endocytosis, ion and pH homeostasis, and for intracellular targeting of vacuolar proteins and vacuolar biogenesis in S. pombe. © Springer-Verlag 2004.

    DOI: 10.1007/s00438-003-0971-7

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  • Pmr1, a P-type ATPase, and Pdt1, an Nramp Homolog, cooperatively regulate in cell morphogenesis in fission yeast: The importance of Mn2+ homeostasis. Reviewed

    Maeda T, Sugiura R, Deng L, He Y, Lu Y, Fujita Y, Takegawa K, Shuntoh H and Kuno T

    Genes to Cells   2004.2

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    DOI: 10.1111/j.1356-9597.2004.00699.x.

  • Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe Reviewed

    Fujita Y., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   68 ( 2 )   306 - 311   2004.2   ISSN:09168451

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    Cysteine synthase catalyzes the formation of cysteine from O-acetylserine, and is the key enzyme for de novo cysteine biosynthesis in Schizosaccharomyces pombe. An examination of the 5. pombe database revealed that two gene products are predicted to encode proteins homologous to eukaryotic cysteine synthases. Disruption of one of these candidates, cys1a+ (SPBC36.04), caused an obvious cysteine auxotrophy, while disruption of cys1b+ (SPAC3A12.17c) had no effect on the growth phenotype. Furthermore, overexpression of cys1b+ did not complement the cysteine auxotrophic phenotype of cys1a mutant cells. These results indicated that cys1a+, not cys1b+, primarily functions in the biosynthesis of cysteine in S. pombe cells. We constructed a bacterial-S. pombe shuttle vector containing cys1a+ as a selective marker gene. The combination of the cysteine auxotroph and new vector could be useful for the expression of a heterologous protein.

    DOI: 10.1271/bbb.68.306

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  • Disruption of the plr1<sup>+</sup> Gene Encoding Pyridoxal Reductase of Schizosaccharomyces pombe Reviewed

    Morita T., Takegawa K., Yagi T.

    Journal of Biochemistry   135 ( 2 )   225 - 230   2004.2   ISSN:0021924X

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    Pyridoxal (PL) reductase encoded by the plr1+ gene practically catalyzes the irreversible reduction of PL by NADPH to form pyridoxine (PN). The enzyme has been suggested to be involved in the salvage synthesis of pyridoxal 5′-phosphate (PLP), a coenzyme form of vitamin B6, or the excretion of PL as PN from yeast cells. In this study, a PL reductase-disrupted (plr1Δ) strain was constructed and its phenotype was examined. The plr1Δ cells showed almost the same growth curve as that of wild-type cells in YNB and EMM media. In EMM, the plr1Δ strain became flocculent at the late stationary phase for an unknown reason. The plr1Δ cells showed low but measurable PL reductase activity catalyzed by some other protein(s) than the enzyme encoded by the plr1+ gene, which maintained the flow of "PL → PN → PNP → PLP" in the salvage synthesis of PLP. The total vitamin B6 and pyridoxamine 5′-phosphate contents in the plr1Δ cells were significantly lower than those in the wild-type ones. The percentages of the PLP amount as to the other vitamin B6 compounds were similar in the two cell types. The amount of PL in the culture medium of the disruptant was significantly higher than that in the wild-type. In contrast, PN was much higher in the latter than the former. The plr1Δ cells accumulated a 6.1-fold higher amount of PL than the wild-type ones when they were incubated with PL. The results showed that PL reductase encoded by the plr1+ gene is involved in the excretion of PL after reducing it to PN, and may not participate in the salvage pathway for PLP synthesis.

    DOI: 10.1093/jb/mvh026

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  • Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast: The importance of Mn<sup>2+</sup> homeostasis Reviewed

    Maeda T., Sugiura R., Kita A., Saito M., Deng L., He Y., Lu Y., Fujita Y., Takegawa K., Shuntoh H., Kuno T.

    Genes to Cells   9 ( 1 )   71 - 82   2004.1   ISSN:13569597

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    Schizosaccharomyces pombe pmr1+ gene is homologous to Saccharomyces cerevisiae PMR1 gene, which encodes the P-type Ca2+/Mn2+-ATPase. Addition of Mn2+, as well as Ca2+, to the medium induced pmr1+ gene expression in a calcineurin-dependent manner. The pmr1 knockout (Δpmr1) cells exhibited hypersensitivity to EGTA. A screen for high gene dosage-suppressors of the EGTA-hypersensitive phenotype of Δpmr1 led to the identification of pdt1+ gene, which encodes an Nramp-related metal transporter. The Δpmr1 cells showed round cell morphology. Although Δpdt1 cells appeared normal in the regular medium, it showed round cell morphology similar to that of the Δpmr1 cells when Mn2+ was removed from the medium. The removal of Mn2+ also exacerbated the round morphology of the Δpmr1 cells. The Δpmr1 Δpdt1 double mutants grew very slowly and showed extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn2+, but not Ca2+, to the medium completely suppressed the morphological defects, while both Mn2+ and Ca2+ markedly improved the slow growth of the double mutants. These results suggest that Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn2+ homeostasis, and that calcineurin functions as a Mn2+ sensor as well as a Mn2+ homeo-stasis regulator.

    DOI: 10.1111/j.1356-9597.2004.00699.x

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  • Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe Reviewed

    Takegawa K., Hosomi A., Iwaki T., Fujita Y., Morita T., Tanaka N.

    Biochemical and Biophysical Research Communications   311 ( 1 )   77 - 82   2003.11   ISSN:0006291X

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    Intracellular vesicle trafficking is mediated by a set of SNARE proteins in eukaryotic cells. Several SNARE proteins are required for vacuolar protein transport and vacuolar biogenesis in Saccharomyces cerevisiae. A search of the Schizosaccharomyces pombe genome database revealed a total of 17 SNARE-related genes. Although no homologs of Vam3p, Nyv1p, and Vam7p have been found in S. pombe, we identified one SNARE-like protein that is homologous to S. cerevisiae Pep12p. However, the disruptants transport vacuolar hydrolase CPY (SpCPY) to the vacuole normally, suggesting that the Pep12 homolog is not required for vacuolar protein transport in S. pombe cells. To identify the SNARE protein(s) involved in Golgi-to-vacuole protein transport, we have deleted four SNARE homolog genes in S. pombe. SpCPY was significantly missorted to the cell surface on deletion of one of the SNARE proteins, Fsv1p (SPAC6F12.03c), with no apparent S. cerevisiae ortholog. In addition, sporulation, endocytosis, and in vivo vacuolar fusion appear to be normal in fsv1Δ cells. These results showed that Fsv1p is mainly involved in vesicle-mediated protein transport between the Golgi and vacuole in S. pombe cells. © 2003 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2003.09.179

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  • Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe. Reviewed International journal

    Takegawa K, Hosomi A, Iwaki T, Fujita Y, Morita T and Tanaka N

    Biochemical and Biophysical Research Communications   2003.10

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    DOI: doi: 10.1016/j.bbrc.2003.09.179.

  • Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in Schizosaccharomyces pombe. Reviewed

    Onishi M, Nakamura Y, Koga T, Takegawa K and Fukui Y

    Bioscience, Biotechnology and Biochemistry   2003.8

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    DOI: doi: 10.1271/bbb.67.1772.

  • Characterization of vps33<sup>+</sup>, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe Reviewed

    Iwaki T., Osawa F., Onishi M., Koga T., Fujita Y., Hosomi A., Tanaka N., Fukui Y., Takegawa K.

    Yeast   20 ( 10 )   845 - 855   2003.7   ISSN:0749503X

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    From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33+ gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33+ gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells. Copyright © 2003 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.1011

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  • Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe. Reviewed International journal

    Iwaki T, Osawa F, Onishi M, Koga T, Fujita Y, Hosomi A, Tanaka N, Fukui Y and Takegawa K

    Yeast   2003.6

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    DOI: doi: 10.1002/yea.1011.

  • Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae. Reviewed International journal

    Takegawa K, Tokudomi S, Bhuiyan MSA, Tabuchi M, Fujita Y, Iwaki T, Utsumi S and Tanaka N

    Current Genetics   2003.4

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    DOI: doi: 10.1007/s00294-002-0357-0.

  • Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharamyces cerevisiae Reviewed

    Takegawa K., Tokudomi S., Bhuiyan M.S.A., Tabuchi M., Fujita Y., Iwaki T., Utsumi S., Tanaka N.

    Current Genetics   42 ( 5 )   252 - 259   2003.2   ISSN:01728083

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    To investigate the intracellular transport mechanism of the vacuolar carboxypeptidase of Schizosaccharomyces pombe (SpCPY), SpCPY was expressed in Saccharomyces cerevisiae and its biosynthesis and sorting were examined. When Sac. cerevisiae prc1Δ, devoid of intrinsic (Sc) CPY activity, was transformed with a plasmid carrying the Sch. pombe cpy1+ gene, CPY activity was restored. Pulse-chase experiments revealed that SpCPY is initially synthesized in a pro-precursor form and then converted to a heterodimer, the mature form, in Sac. cerevisiae cells. SpCPY was not processed into intermediate or mature forms in pep4 mutant cells, indicating that SpCPY was proteolytically cleaved in a PEP4-dependent manner in Sac. cerevisiae. Several vps mutants, which are defective in vacuolar protein-sorting, exhibited a defect in the maturation of SpCPY. Moreover, the maturation of SpCPY was severely inhibited in a vps10 strain, although the pro- segment of SpCPY does not contain a QRPL-like sequence, which is the putative targeting signal of ScCPY. When SpCPY was expressed in a wild-type strain, more than 90% of ScCPY was normally sorted to the vacuole, indicating that SpCPY does not compete with ScCPY for vacuolar sorting.In contrast, expression of SpCPY resulted in a missorting of a ScCPY-invertase fusion protein to the cell surface. These results suggested that there are two different binding sites for SpCPY and ScCPY on Vps10p and that the binding of SpCPY to Vps10p interferes with the binding of a ScCPY-invertase fusion protein.

    DOI: 10.1007/s00294-002-0357-0

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  • Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe. Reviewed International journal

    Onishi M, Koga T, Morita R, Nakamura Y, Nakamura T, Shimoda C, Takegawa K, Hirata A and Fukui Y

    Yeast   2003.2

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    DOI: doi: 10.1002/yea.953.

  • Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in schizosaccharomyce pombe Reviewed

    Onishi M., Nakamura Y., Koga T., Takegawa K., Fukui Y.

    Bioscience, Biotechnology and Biochemistry   67 ( 8 )   1772 - 1779   2003.1   ISSN:09168451

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    The ste12+ gene of Schizosaccharomyces pombe codes for a phosphatidylinositol (PI) 3-phosphate 5′-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12Δ cells were screened for. Most of the mutant genes turned out to be recessive. Six genes were cloned and the open reading frames responsible for the suppressor activity were identified. They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. Disruption of these genes resulted in suppression of the defects of the ste12Δ cells, including low mating efficiency and formation of large vacuoles. Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system. © 2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry.

    DOI: 10.1271/bbb.67.1772

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  • Enzymatic synthesis of neoglycoconjugates by transglycosylation with endo-β-N-acetylglucosaminidase A Reviewed

    Takegawa K., Fan J.

    Methods in Enzymology   362   64 - 74   2003   ISSN:00766879

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    DOI: 10.1016/S0076-6879(03)01006-1

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  • High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1. Reviewed International journal

    Nita-Lazar M, Chevolot L, Iwahara S, Takegawa K, Furmanek A and Lienart Y.

    Acta Biochimica Polonica   2002.10

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  • Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway, and its connection to calcineurin function. Reviewed International journal

    Cheng H, Sugiura R, Wu W, Fujita M, Lu Y, Sio SO, Kawai R, Takegawa K, Shuntoh H and Kuno T.

    Molecular Biology of the Cell   2002.8

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    DOI: doi: 10.1091/mbc.01-09-0463.

  • An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress Reviewed

    Kimura Y., Mishima Y., Nakano H., Takegawa K.

    Journal of Bacteriology   184 ( 13 )   3578 - 3585   2002.6   ISSN:00219193

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    An adenylyl cyclase gene (cyaA) present upstream of an osmosensor protein gene (mokA) was isolated from Myxococcus xanthus, cyaA encoded a polypeptide of 843 amino acids with a predicted molecular mass of 91,187 Da. The predicted cyaA gene product had structural similarity to the receptor-type adenylyl cyclases that are composed of an amino-terminal sensor domain and a carboxy-terminal catalytic domain of adenylyl cyclase. In reverse transcriptase PCR experiments, the transcript of the cyaA gene was detected mainly during development and spore germination. A cyaA mutant, generated by gene disruption, showed normal growth, development, and germination. However, a cyaA mutant placed under conditions of ionic (NaCl) or nonionic (sucrose) osmostress exhibited a marked reduction in spore formation and spore germination. When wild-type and cyaA mutant cells at developmental stages were stimulated with 0.2 M NaCl or sucrose, the mutant cells increased cyclic AMP accumulation at levels similar to those of the wild-type cells. In contrast, the mutant cells during spore germination had mainly lost the ability to respond to high-ionic osmolarity. In vegetative cells, the cyaA mutant responded normally to osmotic stress. These results suggested that M. xanthus CyaA functions mainly as an ionic osmosensor during spore germination and that CyaA is also required for osmotic tolerance in fruiting formation and sporulation.

    DOI: 10.1128/JB.184.13.3578-3585.2002

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  • An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress. Reviewed International journal

    Kimura Y, Mishima Y, Nakano H and Takegawa K.

    Journal of Bacteriology   2002.4

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    DOI: doi: 10.1128/JB.184.13.3578-3585.2002.

  • Transfer of high-mannose type oligosaccharides to disaccharides by endo--N-acetylglucosaminidase from Arthrobacter protophormiae. Reviewed

    2002.2

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  • High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1 Reviewed

    Nita-Lazar M., Chevolot L., Iwahara S., Takegawa K., Furmanek A., Lienart Y.

    Acta Biochimica Polonica   49 ( 4 )   1019 - 1027   2002   ISSN:0001527X

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    So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fractions (named in this work inducer I, II, III) to trigger responses in Rubus protoplasts has been examined. It was found that inducer III was the most efficient as it elicited changes in the levels of phenylpropanoid pathway intermediates in relation to phenylalanine-ammonia lyase (PAL) activation.

    DOI: 10.18388/abp.2002_3762

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  • A novel disaccharide substrate having 1,2-oxazoline moiety for detection of transglycosylating activity of endoglycosidases Reviewed

    Fujita M., Shoda S., Haneda K., Inazu T., Takegawa K., Yamamoto K.

    Biochimica et Biophysica Acta - General Subjects   1528 ( 1 )   9 - 14   2001.9   ISSN:03044165

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    A disaccharide substrate of Manβ1-4GlcNAc-oxazoline 2 was designed and synthesized as a novel probe for detection of the transglycosylating activity of endoglycosidases. A regio- and stereoselective transglycosylation reaction of 2 to GlcNAcβ1-O-pNP or Dns-Asn(GlcNAc)-OH catalyzed by endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) and endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has been demonstrated for the first time, resulting in the core trisaccharide derivative Manβ1-4GlcNAcβ1-4GlcNAcβ1-O-pNP 8 (or -(Dns)Asn-OH). Interestingly, the transglycosylation proceeds irreversibly; the resulting trisaccharide 8 was not hydrolyzed by Endo-M and Endo-A. Based on these results, a new mechanism including an oxazolinium ion intermediate has been proposed for the endoglycosidase-catalyzed hydrolysis or transglycosylation. © 2001 Elsevier Science B.V. All rights reserved.

    DOI: 10.1016/S0304-4165(01)00164-7

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  • Biosynthetic Pathway and Physiological Role of Galactose-Containing Oligosaccharides in Fission Yeast Schizosaccharomyces pombe Reviewed

    Tanaka N., Takegawa K.

    Trends in Glycoscience and Glycotechnology   13 ( 73 )   519 - 532   2001.9   ISSN:09157352

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    In recent years, the fission yeast Schizosaccharomyces pombe has attracted interest as a promising unicellular eukaryote model for studies on the biosynthetic pathway and oligosaccharide structures of glycoproteins. The glycoproteins of S. pombe contain a large amount of galactose in addition to mannose, indicating that S. pombe is equipped with mechanisms for galactosylation of glycoprotein, like mammalian cells. To elucidate the physiological role of galactosylation, we isolated an S. pombe mutant (gmsl) defective in protein galactosylation. We found that disruption of the ginsl+ gene (Δgmsl) in S. pombe led to a complete loss of cell surface galactosylation, due to a defect in the transport of UDP-galactose as substrate for galactosyltransferase from cytosol into the lumen of the Golgi apparatus. Therefore, the Δgmsl strain is very useful for the analysis of the phenotypes of S. pombe cells lacking galactose residues and for the elucidation of the galactosylation mechanism. Although galactose residues are not essential for growth of S. pombe cells, the galactosylation of protein is required for the maintenance of normal cell shape, sexual agglutination, tolerance toward various drugs, and non-sexual flocculation in S. pombe.

    DOI: 10.4052/tigg.13.519

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  • Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity Reviewed

    Tanaka N., Konomi M., Osumi M., Takegawa K.

    Yeast   18 ( 10 )   903 - 914   2001.8   ISSN:0749503X

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    In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1+, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Δgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1+ was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with β-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δgms1 cells, while other phenotypes were weakly suppressed. The Δgms1 (h90) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe. Copyright © 2001 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.740

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  • A novel disaccharide substrate having 1,2-oxazoline moiety for detection of transglycosylating activity of endoglycosidases. Reviewed International journal

    Fujita M, Shoda S, Haneda K, Inazu T, Takegawa K and Yamamoto K

    Biochimica et Biophysica Acta   2001.8

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    DOI: doi: 10.1016/s0304-4165(01)00164-7.

  • Identification of amino acid residues essential for the substrate specificity of Flavobacterium sp. Endo-β-N-acetylglucosaminidase Reviewed

    Fujita K., Nakatake R.I., Yamabe K., Watanabe A., Asada Y., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   65 ( 7 )   1542 - 1548   2001.7   ISSN:09168451

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    The gene encoding the endo-β-N-acetylglucosaminidase from Flavobacterium sp. (Endo-Fsp) was sequenced. The Endo-Fsp gene was overexpressed in Escherichia coli cells, and was purified from inclusion bodies after denaturation by 8 M urea. The renatured Endo-Fsp had the same optimum pH and substrate specificity as the native enzyme. Endo-Fsp had 60% sequence identity with the endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H), and the putative catalytic residues were conserved. Site-directed mutagenesis was done at conserved residues based on the three-dimensional structure and mutagenesis of Endo-H. The mutant of Glu-128, corresponding to Glu-132 in Endo-H and identified as an active site residue, was inactivated. Mutagenesis around the predicted active site of Endo-Fsp reduced the enzymatic activity. Moreover, the hydrolytic activity toward hybrid-type oligosaccharides was decreased compared to that toward high-mannose type oligosaccharides by mutagenesis of Asp-126 and Asp-127. Therefore, site-directed mutagenesis of some of these conserved residues indicates that the predicted active sites are essential to the enzymatic activity of Endo-Fsp, and may have similar roles in catalysis as their counterparts in Endo-H.

    DOI: 10.1271/bbb.65.1542

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  • Functional characterization of Gms1p/UDP-galactose transporter in Schizosaccharomyces pombe Reviewed

    Tanaka N., Takegawa K.

    Yeast   18 ( 8 )   745 - 757   2001.6   ISSN:0749503X

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    Galactosylation of glycoproteins in the fission yeast Schizosaccharomyces pombe requires the transport of UDP-galactose as substrate for the galactosyltransferase into the lumen of the Golgi apparatus, which is achieved by the UDP-galactose transporter. We isolated a mutant (gms1) that is deficient in galactosylation of cell surface glycoproteins in Sz. pombe, and found that the gms1+ gene encodes a UDP-galactose transporter. In the prediction of secondary structure of the Gms1 protein, an eight-membrane-spanning structure was obtained. Fluorescent microscopy revealed the functional Gms1-GFP fusion protein to be stably localized at the Golgi membrane. Sequencing analysis of the coding region of Gms1p derived from galactosylation-defective mutants identified a single amino acid mutation (A102T or A258E) located within the putative transmembrane region, helix 2 or helix 7, respectively. The mutagenized Gms1(A102T or A258E)p exhibited loss of UDP-galactose transport activity but no change in the localization to the Golgi membrane. The C-terminal truncated Gms1p mutants demonstrated that the C-terminal hydrophilic region was dispensable for targeting and function as UDP-galactose transporter at the Golgi membrane. We suggest that the putative eighth (the most C-terminus-proximal) transmembrane helix of Gms1p is critical to targeting from ER to the Golgi membrane. Copyright © 2001 John Wiley & Sons, Ltd.

    DOI: 10.1002/yea.725

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  • Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity. Reviewed International journal

    Tanaka N, Konomi M, Osumi M and Takegawa K

    Yeast   2001.6

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    DOI: doi: 10.1002/yea.740.

  • Identification of amino acid residues essential for the substrate specificity of Flavobacterium sp. endo-beta-N-acetylglucosaminidase. Reviewed

    Fujita K, Nakatake R, Yamabe K, Watanabe A, Asada Y and Takegawa K

    Bioscience, Biotechnology, and Biochemistry   2001.6

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    DOI: doi: 10.1271/bbb.65.1542.

  • Nystatin sensitive and vacuolar protein sorting defective (vps) mutants of Saccharomyces cerevisiae: Their isolation and characterization. Reviewed International journal

    Bhuiyan MSA, Ito Y, Tanaka N, Sadik G, Fujita K, Nakamura A, Biswas MH, Fukui H and Takegawa K

    Pakistan Journal of Biological Sciences   2001.5

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  • Functional characterization of Gms1p/UDP-galactose transporter in Schizosaccharomyces pombe. Reviewed International journal

    Tanaka N and Takegawa K

    Yeast   2001.5

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    DOI: doi: 10.1002/yea.725.

  • Functional characterization of alanine racemase from Schizosaccharomyces pombe: A eucaryotic counterpart to bacterial alanine racemase Reviewed

    Uo T., Yoshimura T., Tanaka N., Takegawa K., Esaki N.

    Journal of Bacteriology   183 ( 7 )   2226 - 2233   2001.4   ISSN:00219193

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    Schizosaccharomyces pombe has an open reading frame, which we named alr1+, encoding a putative protein similar to bacterial alanine racemase. We cloned the alr1+ gene in Escherichia coli and purified the gene product (Alr1p), with an Mr of 41,590, to homogeneity. Alr1p contains pyridoxal 5′-phosphate as a coenzyme and catalyzes the racemization of alanine with apparent Km and Vmax values as follows: for L-alanine, 5.0 mM and 670 μmol/min/mg, respectively, and for D-alanine, 2.4 mM and 350 μmol/min/mg, respectively. The enzyme is almost specific to alanine, but L-serine and L-2-aminobutyrate are racemized slowly at rates 3.7 and 0.37% of that of L-alanine, respectively. S. pombe uses D-alanine as a sole nitrogen source, but deletion of the alr1+ gene resulted in retarded growth on the same medium. This indicates that S. pombe has catabolic pathways for both enantiomers of alanine and that the pathway for L-alanine coupled with racemization plays a major role in the catabolism of D-alanine. Saccharomyces cerevisiae differs markedly from S. pombe: S. cerevisiae uses L-alanine but not D-alanine as a sole nitrogen source. Moreover, D-alanine is toxic to S. cerevisiae. However, heterologous expression of the alr1+ gene enabled S. cerevisiae to grow efficiently on D-alanine as a sole nitrogen source. The recombinant yeast was relieved from the toxicity of D-alanine.

    DOI: 10.1128/JB.183.7.2226-2233.2001

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  • Tryptophan-216 is essential for the transglycosylation activity of endo--N-acetylglucosaminidase A. Reviewed International journal

    2001.4

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    DOI: doi: 10.1006/bbrc.2001.4836

  • Functional characterization of alanine racemase from Schizosaccharomyces pombe: A eucaryotic counterpart to bacterial alanine racemase. Reviewed International journal

    Uo T, Yoshimura T, Tanaka N, Takegawa K and Esaki N

    Journal of Bacteriology   2001.3

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    DOI: doi: 10.1128/JB.183.7.2226-2233.2001

  • Chemoenzymatic synthesis of neoglycoproteins using transglycosylation with endo-beta-N-acetylglucosaminidase A. Reviewed International journal

    Fujita K and Takegawa K

    Biochemical and Biophysical Research Communications   2001.3

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    DOI: doi: 10.1006/bbrc.2001.4631

  • Myxococcus xanthus mokA encodes a histidine kinase-response regulator hybrid sensor required for development and osmotic tolerance Reviewed

    Kimura Y., Nakano H., Terasaka H., Takegawa K.

    Journal of Bacteriology   183 ( 4 )   1140 - 1146   2001.2   ISSN:00219193

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    A gene, mokA, encoding a protein with similarities to histidine kinase-response regulator hybrid sensor, was cloned from a Myxococcus xanthus genomic library. The predicted mokA gene product was found to contain three domains: an amino-terminal input domain, a central transmitter domain, and a carboxy-terminal receiver domain, mokA mutants placed under starvation conditions exhibited reduced sporulation. Mutation of mokA also caused marked growth retardation at high osmolarity. These results indicated that M. xanthus MokA is likely a transmembrane sensor that is required for development and osmotic tolerance. The putative function of MokA is similar to that of the hybrid histidine kinase, DokA, of the eukaryotic slime mold Dictyostelium discoideum.

    DOI: 10.1128/JB.183.4.1140-1146.2001

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  • Myxococcus xanthus mokA encodes a histidine kinase-response regulator hybrid sensor required for development and osmotic torelance. Reviewed International journal

    Kimura Y, Nakano H, Terasaka H and Takegawa K

    Journal of Bacteriology   2001.2

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    DOI: doi: 10.1128/JB.183.4.1140-1146.2001

  • Chemoenzymatic synthesis of neoglycoproteins using transglycosylation with endo-β-N-acetylglucosaminidase A Reviewed

    Fujita K., Takegawa K.

    Biochemical and Biophysical Research Communications   282 ( 3 )   678 - 682   2001   ISSN:0006291X

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    A novel chemoenzymatic approach to synthesize neoglycoproteins containing high-mannose-type oligosaccharides is described. p-Isothiocyanatophenyl-β-D-glucopyranoside (Glc-ITC) was transferred to the reducing end of the high-mannose-type oligosaccharides using a transglycosylation activity of endo-β-N-acetylglucosaminidase A (Endo-A). A novel oligosaccharide, Man6GlcNAc-Glc-ITC, was synthesized as a coupling reagent for lysyl and N-terminal residues of the protein moiety. The neoglycoconjugate was coupled with several nonglycosylated proteins such as ribonuclease A, lysozyme, and α-lactalbumin. Between one and four high-mannose-type oligosaccharides were incorporated per molecule of these proteins. This method should be very useful for the synthesis of neoglycoproteins with homogeneous high-mannose-type oligosaccharides. © 2001 Academic Press.

    DOI: 10.1006/bbrc.2001.4631

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  • Plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase Reviewed

    Fujita K., Asada Y., Yamamoto K., Takegawa K.

    Journal of Bioscience and Bioengineering   90 ( 4 )   462 - 464   2000.10   ISSN:13891723

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    The transglycosylation activity of Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A) was used for the enzymatic synthesis of a novel oligosaccharide, Man6GlcNAc-5-bromo-4-chloro-3-indolyl-β-glucoside (Man6GlcNAc-Glc-β-X). Various endo-β-N-acetylglucosaminidases hydrolyzed this oligosaccharide, producing Man6GlcNAc and Glc-β-X. The E. coli strains coexpressing Endo-A and β-glucosidase formed blue colonies in the presence of Man6GlcNAc-Glc-β-X. Therefore, endo-β-N-acetylglucosaminidase activity could be directly detected by the plate assay. This simple plate assay is useful for screening microorganisms for endo-β-N-acetylglucosaminidase activity.

    DOI: 10.1016/S1389-1723(01)80021-9

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  • A plate assay for endo-beta-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-beta-N-acetylglucosaminidase. Reviewed

    Fujita K, Asada Y, Yamamoto K and Takegawa K

    Journal of Bioscience and Bioengineering   2000.8

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  • Synthesis of neoglycoenzymes with homogeneous N-linked oligosaccharides using immobilized endo-beta-N-acetylglucosaminidase A. Reviewed International journal

    Fujita K, Tanaka N, Sano M, Kato I, Asada Y and Takegawa K

    Biochemical and Biophysical Research Communications   2000.6

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    DOI: doi: 10.1006/bbrc.1999.1963.

  • The active oxygen responce of raspberry protoplasts to O-glycans of Fusarium sp. M7-1. Reviewed International journal

    Nita-Lazar M, Iwahara S, Takegawa K and Lienart Y

    Journal of Plant Physiology   2000.4

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  • Role of oligosaccharides in microbial glycoproteins and synthetic methods of neoglycoproteins Reviewed

    Takegawa K.

    Nippon Nogeikagaku Kaishi   74 ( 11 )   1237 - 1246   2000   ISSN:00021407

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    DOI: 10.1271/nogeikagaku1924.74.1237

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  • Functional analysis of the human NRAMP family expressed in fission yeast Reviewed

    Tabuchi M., Yoshida T., Takegawa K., Kishi F.

    Biochemical Journal   344 ( 1 )   211 - 219   1999.11   ISSN:02646021

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    The Bcg/Ity/Lsh locus in the mouse genome regulates macrophage activation for antimicrobial activity against intracellular pathogens, and the positional cloning of this locus identified the Nramp1 (natural resistance-associated macrophage protein) gene. Nramp2 was initially isolated as a homologue of Nramp1. Recently, the rat divalent metal transporter DMT1 was identified electrophysiologically, and was found to be an isoform of Nramp2, a mutation which was subsequently identified in rats suffering from hereditary iron-deficiency anaemia. Despite the 64% amino acid sequence identity of Nramp1 and Nramp2, no divalent metal transport activity has yet been detected from Nramp1, and the function of Nramp1 on the molecular level is still unclear. To investigate the divalent metal transport activity of NRAMP molecules, we constructed four chimeric NRAMP genes by swapping the domains of human NRAMP1 and NRAMP2 with each other. The functional characteristics of wild-type NRAMP1, NRAMP2 and their chimeras were determined by expression in the divalent metal transporter-disrupted strain of fission yeast, pdt1Δ, and we analysed the divalent metal transport activity by complementation of the EGTA- and pH-sensitive phenotype of pdt1Δ. Replacement of the N-terminal cytoplasmic domain of NRAMP2 with the NRAMP1 counterpart resulted in inactive chimeras, indicating that the functional difference between NRAMP1 and NRAMP2 is located in this region. However, results obtained with the reverse construct and other chimeras indicated that these regions are not solely responsible for the differences in EGTA- and pH-sensitivity of NRAMP1 and NRAMP2. These findings indicate that NRAMP1 itself cannot represent the divalent metal transport activity in S. pombe and the additional protein segments of the molecules located elsewhere in NRAMP1 are also functionally distinct from their NRAMP2 counterparts.

    DOI: 10.1042/0264-6021:3440211

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  • Functional analysis of the human NRAMP family expressed in fission yeast. Reviewed International journal

    Tabuchi M, Yoshida T, Takegawa K and Kishi F

    Biochemical Journal   1999.9

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  • Schizosaccharomyces pombe UDP-galactose transporter: identification of its functional form through cDNA cloning and expression in mammalian cells. Reviewed International journal

    Segawa H, Ishida N, Takegawa K and Kawakita M

    FEBS Letters   1999.7

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    DOI: doi: 10.1016/s0014-5793(99)00596-7.

  • Cell-surface galactosylation is essential for non-sexual flocculation in Schizosaccharomyces pombe. Reviewed International journal

    Tanaka N, Awai A, Bhuiyan MSA, Fujita K, Fukui H and Takegawa K

    Journal of Bacteriology   1999.5

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    DOI: doi: 10.1128/JB.181.4.1356-1359.1999.

  • Nystatin effects on vacuolar function in Saccharomyces cerevisiae. Reviewed International journal

    Bhuiyan MSA, Ito Y, Nakamura A, Tanaka N, Fujita K, Fukui H and Takegawa K

    Bioscience, Biotechnology, and Biochemistry   1999.5

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    DOI: doi: 10.1271/bbb.63.1075.

  • Chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosaccharide by endo--N-acetylglucosaminidase from Arthrobacter protophormiae. Reviewed

    1999.3

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  • Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe Reviewed

    Tanaka N., Awai A., Bhuiyan M.S.A., Fujita K., Fukui H., Takegawa K.

    Journal of Bacteriology   181 ( 4 )   1356 - 1359   1999.2   ISSN:00219193

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    We have isolated fission yeast mutants that constitutively flocculate upon growth in liquid media. One of these mutants, the gsf1 mutant, was found to cause dominant, nonsexual, and calcium-dependent aggregation of cells into flocs. Its flocculation was inhibited by the addition of galactose but was not affected by the addition of mannose or glucose, unlike Saccharomyces cerevisiae FLO mutants. The gsf1 mutant coflocculated with Schizosaccharomyces pombe wild-type cells, while no coflocculation was found with galactose-deficient (gms1Δ) cells. Moreover, flocculation of the gsf1 mutant was also inhibited by addition of cell wall galactomannan from wild- type cells but not from gms1Δ cells. These results suggested that galactose residues in the cell wall glycoproteins may be receptors of gsf1-mediated flocculation, and therefore cell surface galactosylation is required for nonsexual flocculation in S. pombe.

    DOI: 10.1128/jb.181.4.1356-1359.1999

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  • Nystatin effects on vacuolar function in saccharomyce Reviewed

    Bhuiyan M.S.A., Ito Y., Nakamura A., Tanaka N., Fujita K., Fukui H., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   63 ( 6 )   1075 - 1082   1999.1   ISSN:09168451

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    The effects of nystatin, a polyene antibiotic, was studied in Saccharomyces cerevisiae by isolating and characterizing nystatin-sensitive mutants. We isolated a number of nystatin-sensitive mutants by ethylmethane sulfonate mutagenesis. One of these mutants, the nss1 mutant, was characterized in detail. The mutant was sensitive to stresses such as high temperature or high concentrations of monovalent and divalent cations. The nss1 mutants showed severe vacuolar protein sorting and vacuolar morphology defects. The nss1 mutant was demonstrated to have a mutational lesion in the known VPS16 gene, which is essential for vacuolar protein sorting in S. cerevisiae. All of the vacuolar deficient mutants (vps11, vps16, vps18, and vps33) were sensitive to nystatin. Nystatin was found to cause extensive enlargement of the vacuole in wild-type S. cerevisiae cells. These results are discussed with special reference to the vacuolar function of S. cerevisiae. © 1999, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.63.1075

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  • Chemo-enzymatic synthesis of a calcitonin derivative containing a high- mannose type oligosaccharide by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae Reviewed

    Yamamoto K., Haneda K., Iguchi R., Inazu T., Mizuno M., Takegawa K., Kondo A., Kato I.

    Journal of Bioscience and Bioengineering   87 ( 2 )   175 - 179   1999   ISSN:13891723

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    Chemo-enzymatic addition of a high-mannose type oligosaccharide to eel calcitonin (CT), a calcium-regulating hormone, was examined. The endo-β-N- acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) transglycosylated the Man6-GlcNAc moiety from an ovalbumin-derived high- mannose type glycosyl asparagine, Asn(Man6-GlcNAc2)-OH, to a calcitonin derivative, [Asn(GlcNAc)3)-CT, in which the N-acetyl-D-glucosamine (GlcNAc) is attached to the third L-asparagine (Asn) residue of the peptide, and a calcitonin derivative containing a high-mannose type oligosaccharide, [Asn(Man6-GlcNAc2)3]-CT, was synthesized. The optimal reaction conditions for the synthesis of [Asn(Man6-GlcNAc2)3]-CT from Asn(Man6-GlcNAc2)-OH and [Asn(GlcNAc)3]-CT catalyzed by Endo-A were examined. The transglycosylation yield relative to the concentration of the [Asn(GlcNAc)3- CT added was 32.7%, and 4.42 mg of [Asn(Man6-GlcNAc2)3]-CT was prepared.

    DOI: 10.1016/S1389-1723(99)89008-2

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  • Isolation and characterization of high-osmolarity-sensitive mutants of fission yeast Reviewed

    Aiba H., Kawaura R., Yamamoto E., Yamada H., Takegawa K., Mizuno T.

    Journal of Bacteriology   180 ( 19 )   5038 - 5043   1998.10   ISSN:00219193

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    For the fission yeast Schizosaccharomyces pombe, adaptation to high- osmolarity medium is mediated by a mitogen-activated protein (MAP) kinase cascade, involving the Wis1 MAP kinase kinase and the Sty1 MAP kinase. The MAP kinase pathway transduces an osmotic signal and accordingly regulates the expression of the downstream target gene (gpd1+) that encodes NADH-dependent glycerol-3-phosphate dehydrogenase, in order to adaptively accumulate glycerol inside the cells as an osmoprotectant. We previously characterized a set of high-osmolarity-sensitive S. pombe mutants, including wis1, sty1, and gpd1. In this study, we attempted to further isolate novel osmolarity- sensitive mutants. For some of the mutants isolated, profiles of glycerol production in response to the osmolarity of the growth medium were indistinguishable from that of the wild-type cells, suggesting that they are novel types. They were classified into three distinct types genetically and, thus, were designated hos1, hos2, and hos3 (high osmolarity sensitive) mutants. One of them, the hos1 mutant, was characterized in detail. The hos1 mutant was demonstrated to have a mutational lesion in the known ryh1+ gene, which encodes a small GTP-binding protein. Disruption of the ryh1+ gene results not only in osmosensitivity but also in temperature sensitivity for growth. It was also found that the Δryh1 mutant is severely sterile. These results are discussed with special reference to the osmoadaptation of S. pombe.

    DOI: 10.1128/jb.180.19.5038-5043.1998

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  • Isolation and characterization of high-osmolarity-sensitive mutants in fission yeast. Reviewed International journal

    Aiba H, Kawaura R, Yamamoto E, Yamada H, Takegawa K and Mizuno T

    Journal of Bacteriology   1998.7

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    DOI: doi: 10.1128/JB.180.19.5038-5043.1998.

  • Isolation and characterization of an invertase and its repressor genes from Schizosaccharomyces pombe. Reviewed International journal

    Tanaka N, Ohuchi N, Osaka Y, Mukai Y, Ohtani Y, Tabuchi M, Bhuiyan MSA, Fukui H, Harashima S and Takegawa K

    Biochemical and Biophysical Research Communications   1998.5

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    DOI: doi: 10.1006/bbrc.1998.8406.

  • Synthesis of a high-mannose-type oligosaccharide glycopeptide analog containing a glucose-asparagine linkage. Reviewed International journal

    Deras IL, Takegawa K, Kondo A, Kato I and Lee YC

    1998.5

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    DOI: doi: 10.1016/s0960-894x(98)00306-0.

  • Isolation and characterization of an invertase and its repressor genes from Schizosaccharomyces pombe Reviewed

    Tanaka N., Ohuchi N., Mukai Y., Osaka Y., Ohtani Y., Tabuchi M., Bhuiyan M.S.A., Fukui H., Harashima S., Takegawa K.

    Biochemical and Biophysical Research Communications   245 ( 1 )   246 - 253   1998.4   ISSN:0006291X

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    PCR was used to isolate an invertase homolog gene from the fission yeast Schizosaccharomyces pombe. The cloned inv1+ gene encodes a protein of 581 amino acids with 16 potential asparagine-linked glycosylation sites, and has 39% and 38% identity to the Schwanniomyces occidentalis and Saccharomyces cerevisiae SUC2 invertases. When the inv1+ gene was disrupted, S. pombe strains lacked detectable invertase activity. This result showed that the inv1+ gene encodes only one active invertase in S. pombe cells. The transcription of inv1+ is repressed in the presence of glucose. The transcription of inv1+ was not affected in cyr1Δ strain which lacks adenylate cyclase activity, unlike transcription of S. pombe fbp1+ gene. We have identified an S. pombe gene (scr1+) that encodes a homolog of the Aspergillus nidulans CREA which is required for glucose repression of the glyconeogenic pathway. Although the deletion of scr1+ did not influence the transcription of fbp1+ gene, glucose repression of the inv1+ gene was severely affected. These results showed that glucose repression of inv1+ gene is dependent on scr1+ gene, and S. pombe cAMP signalling pathway may not be essential for glucose repression of inv1+ gene.

    DOI: 10.1006/bbrc.1998.8406

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  • Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase: A substrate for colorimetric detection of endo-β-N-acetylglucosaminidase activity Reviewed

    Takegawa K., Fujita K., Fan J.Q., Tabuchi M., Tanaka N., Kondo A., Iwamoto H., Kato I., Lee Y.C., Iwahara S.

    Analytical Biochemistry   257 ( 2 )   218 - 223   1998.3   ISSN:00032697

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    The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of a novel oligosaccharide, Man6GlcNAc-p-nitrophenylα-D-glucose (Man6GlcNAc-Glc- pNP). The reaction was efficiently induced in aqueous solution containing dimethyl sulfoxide. In the medium containing 20% (v/v) dimethyl sulfoxide with 0.1 M Glc-pNP as an acceptor, the transglycosylation attained yields of 75% by high-performance anion-exchange chromatography. The structure of Man6GlcNAc-Glc-pNP was confirmed by ion mass spectrometry and 400 MHz 1H NMR specrometry. Various endo-β-N-acetylglucosaminidases hydrolyzed this oligosaccharide and Man6GlcNAc and Glc-pNP were released from the oligosaccharide by endo-β-N-acetylglucosaminidase digestion. We have established a new procedure for the colorimetric detection of endo-βN- acetylglucosaminidase activity using Man6GlcNAc-Glc-pNP, which is simple as that for other exoglycosidase assays with pNP-glycosides as substrates.

    DOI: 10.1006/abio.1997.2543

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  • Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-beta-N-acetylglucosaminidase: a substrate for colorimetric detection of endo-beta-N-acetylglucosaminidase activity. Reviewed International journal

    Takegawa K, Fujita K, Fan J-Q, Tabuchi M, Tanaka N, Kondo A, Iwamoto H, Kato I, Lee YC and Iwahara S

    Analytical Biochemistry   1998.3

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    DOI: doi: 10.1006/abio.1997.2543.

  • Production and characterization of extracellular uronic acid containing-glycoproteins from Fusarium oxysporum. Reviewed

    Takegawa K, Satoh K, Nahrowi R, Jikibara T and Iwahara S

    Journal of Fermentation and Bioengineering   1997.5

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  • Vacuolar protein sorting in fission yeast: cloning, biosynthesis, transport, and processing of carboxypeptidase Y from Schizosaccharomyces pombe. Reviewed International journal

    Tabuchi M, Iwaihara O, Ohtani Y, Ohuchi N, Sakurai J, Morita T, Iwahara S and Takegawa K

    Journal of Bacteriology   1997.5

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    DOI: doi: 10.1128/jb.179.13.4179-4189.1997.

  • The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation. Reviewed International journal

    Tabuchi M, Tanaka N, Iwahara S and Takegawa K

    Biochemical and Biophysical Research Communications   1997.5

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    DOI: doi: 10.1006/bbrc.1997.6239.

  • Cloning, sequencing, and expression of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in Escherichia coli. Reviewed International journal

    Takegawa K, Yamabe K, Fujita K, Tabuchi M, Mita M, Watanabe A, Asada Y, Sano M, Kondo A, Kato I and Iwahara S

    Archives of Biochemistry and Biophysics   1997.3

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    DOI: doi: 10.1006/abbi.1996.9803.

  • Cloning, sequencing, and expression of Arthrobacter protophormiae Endo- β-N-acetylglucosaminidase in Escherichia coli Reviewed

    Takegawa K., Yamabe K., Fujita K., Tabuchi M., Mita M., Izu H., Watanabe A., Asada Y., Sano M., Kondo A., Kato I., Iwahara S.

    Archives of Biochemistry and Biophysics   338 ( 1 )   22 - 28   1997.2   ISSN:00039861

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    The gene encoding endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) was cloned, and its nucleotide sequence was determined. A single open reading frame consisting of 1935 base pairs and encoding a polypeptide composed of signal peptides of 24 amino acids and a mature protein of 621 amino acids was found. The primary structure of Endo-A exhibited significant homology with F01F. 10 gene product from Caenorhabditis elegans and weak homology with peptide-N4-(N-acetyl-β-D- glucosaminyl)asparagine amidase from Flavobacterium meningosepticum and chitinase from Streptomyces olivaceoviridis. However, the enzyme had no significant homology with any previously reported endo-β-N- acetylglucosaminidases. Transformed Escherichia coli cells carrying the 4.5- kb fragment expressed Endo-A activity. This enzyme activity was detected in the medium as well as in the periplasmic space of cells under the control of the Endo-A gene promoter. The recombinant Endo-A was efficiently isolated from the periplasmic space of the cells. N-terminal sequence analysis revealed that native and recombinant Endo-A have the same N-terminus. Recombinant and native Endo-A also showed very similar optimum pH profiles and transglycosylation activity.

    DOI: 10.1006/abbi.1996.9803

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  • Production and characterization of extracellular uronic acid-containing glycoproteins from Fusarium oxysporum Reviewed

    Takegawa K., Satoh K., Ramli N., Jikibara T., Iwahara S.

    Journal of Fermentation and Bioengineering   83 ( 2 )   197 - 200   1997   ISSN:0922338X

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    Fusarium and Giberella species were found to secrete acidic polysaccharides when grown in Czapex-Dox medium. Fusarium oxysporum showed the highest rate of secretion of acidic sugars into the growth medium. The acidic sugar-containing glycoprotein from F. oxysporum was purified from the culture filtrate to apparent homogeneity on ultracentrifugation analysis, by ammonium sulfate precipitation, Toyopearl HW-55 gel filtration and charcoal chromatography. The purified acidic sugar-containing glycoprotein has a 10% protein content and a content of 40% acidic sugars as glucuronic acid, and the neutral sugars present in this glycoprotein are mainly mannose, galactose, and glucose. The protein moiety of the glycoprotein contains a high proportion of serine and threonine residues. Treatment of the glycoprotein with alkaline solution resulted in an increase in absorbance at 241 nm, and alkaline borohydride treatment resulted in a marked decrease in the number of serine and threonine residues. We conclude that the glycoprotein has O-linked sugar chains which are mainly attached to serine and threonine residues of the protein moiety. The primary structure of the acidic polysaccharide from F. oxysporum was analyzed mainly by NMR spectrometry. The main parts of this polysaccharide have structures similar to those of uronic acid-containing polysaccharide from Fusarium sp. M7-1.

    DOI: 10.1016/S0922-338X(97)83583-0

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  • Isolation and characterization of a glycosylation mutant from Schizosaccharomyces pombe. Reviewed

    Takegawa K, Tanaka N, Tabuchi M and Iwahara S

    Bioscience, Biotechnology, and Biochemistry   1996.7

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    DOI: doi: 10.1271/bbb.60.1156.

  • Transfer of Man9GlcNAc to L-fucose by endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae. Reviewed International journal

    Fan J-Q, Huynh LH, Reinhold BB, Reinhold VN, Takegawa K, Iwahara S, Kondo A, Kato I and Lee YC

    Glycoconjugate Journal   1996.5

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    DOI: doi: 10.1007/BF00731453.

  • Isolation and identification of a choline-linked mannobiose in the glycoproteins of Fusarium sp. M7-1. Reviewed

    Iwahara S, Suemori N and Takegawa K

    Bioscience, Biotechnology, and Biochemistry   1996.3

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    DOI: doi: 10.1271/bbb.60.349.

  • Isolation and identification of a choline-linked mannobiose in the glycoproteins of fusarium sp. M7-1 Reviewed

    Iwahara S., Suemori N., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   60 ( 2 )   349 - 350   1996.1   ISSN:09168451

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    An unidentified oligosaccharide was isolated from an oligomer mixture derived by alkaline borohydride treatment from glycoproteins of Fusarium sp. M7-1. The isolated compound was identified as O-α-d-Mannopyranosyl (1 → 2)-d-Mannitol-6-phosphocholine by NMR and Ms spectrometry. © 1996, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.349

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  • Degradation of β1→6 Galactofuranoside Linkages in the Polysaccharide of Fusarium sp. M7-1 by Endo-β-galactofuranosidase from Bacillus sp Reviewed

    Iwahara S., Mishima T., Ramli N., Takegawa K.

    Bioscience, Biotechnology and Biochemistry   60 ( 6 )   957 - 961   1996.1   ISSN:09168451

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    A polysaccharide, in which the main part of the side chains were depleted, was prepared from the acidic polysaccharides of Fusarium sp. M7–1 by digestion with lyase of Cellulomonas sp. and mild acid treatment. This polysaccharide was degraded into several fragments, neutral oligosaccharides, neutral polysaccharide, and acidic polysaccharide, by an enzyme, endo-β -galactofuranosidase, produced by Bacillus sp. The main components of the oligosaccharides were isolated and identified as Galf β→6Gal, Glαxl→2Galfβ1→6Gal fβ1→ Glcxl →2Gal fβ1 →6Gal fβ1 →6Gal, Glcxl →2Galfβ 1 →6(Glcαl→2)Gal fβ1 →6Gal and Glcαl →2Ga fβ1 →6(Glcαl → 2)Gal fβ1 →6Galfβ1 →6Gal. The molecular mass of the neutral polysaccharide fragment was estimated to be about 6000 Da by gel filtration chromatography. The polysaccharide fragment consisted of an α1→6 linked mannan main chain to which various sugars, namely Glc, Man, and Rha were attached through α1→3 (or 2) linkages. The molecular mass of the acidic polysaccharide fragment was estimated to be about 6000 Da from the amounts of the reducing terminal galactose. The chemical structures of the oligosaccharides derived from the acidic polysaccharide fragment by mild acid hydrolysis were identified as reported structural units [Iwahara et al., J. Biochem., 112, 355–359 (1992)]. The structure of the mild-acid-resistant part of the acidic polysaccharide fragment was assumed to be a polyuronide to which various sugars such as Glc, Man, and GlcNAc are attached as the side chains. The linkage modes of each sugar are not clear. © 1996, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.957

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  • Isolation and characterization of a glycosylation mutant from schizosaccharomyces pombe Reviewed

    Takegawa K., Tanaka N., Tabuchi M., Iwahara S.

    Bioscience, Biotechnology and Biochemistry   60 ( 7 )   1156 - 1159   1996.1   ISSN:09168451

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    N-Linked oligosaccharides were elongated by glycosylation with mannose and galactose residues in the secretory pathway of Schizosaccharomyces pombe. The wild-type S. pombe cells were agglutinated by the additions of not only concanavalin A lectin, which is specific for mannose residues, but also PNA (from Arachis hypogaea) and RCA (Ricinus communis) lectins, which are specific for terminal galactose residues. By PNA-binding selection, we isolated an S. pombe mutant defective in protein glycosylation. The mutant cells, named gmsl, were not agglutinated by PNA or RCA. In contrast, agglutination of the gmsl cells by the addition of concanavalin A was markedly increased. Structural studies on N-linked oligosaccharides from gmsl mutant cells showed that the number of x-l,2-linked galactose residues wes markedly reduced, and unsubstituted x-l,6-linked polymannose outer chains were attached to the core oligosaccharides. © 1996, Taylor & Francis Group, LLC. All rights reserved.

    DOI: 10.1271/bbb.60.1156

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  • Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology. Reviewed International journal

    Takegawa K, DeWald DB and Emr SD

    Journal of Cell Science   1995.10

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    DOI: doi: 10.1242/jcs.108.12.3745.

  • A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: Biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development Reviewed

    Zhou K., Takegawa K., Emr S.D., Firtel R.A.

    Molecular and Cellular Biology   15 ( 10 )   5645 - 5656   1995.10   ISSN:02707306

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    Three groups of phosphatidylinositol (PI) kinases convert PI into PI(3)phosphate, PI(4)phosphate, PI(4,5) bisphosphate, and PI(3,4,5)trisphosphate. These phosphoinositides have been shown to function in vesicle-mediated protein sorting, and they serve as second-messenger signaling molecules for regulating cell growth. To further elucidate the mechanism of regulation and function of phosphoinositides, we cloned genes encoding five putative PI kinases from Dictyostelium discoideum. Database analysis indicates that D. discoideum PIK1 (DdPIK1), -2, and -3 are most closely related to the mammalian p110 PI 3-kinase, DdPIK5 is closest to the yeast Vps34p PI 3-kinase, and DdPIK4 is most homologous to PI 4-kinases. Together with other known PI kinases, a superfamily of PI kinase genes has been defined, with all of the encoded proteins sharing a common highly conserved catalytic core domain. DdPIK1, -2, and -3 may have redundant functions because disruption of any single gene had no effect on D. discoideum growth or development. However, strains in which both of the two most highly related genes, DdPIK1 and DdPIK2, were disrupted showed both growth and developmental defects, while double knockouts of DdPIK1 and DdPIK3 and DdPIK2 and DdPIK3 appear to be lethal. The ΔDdpik1 ΔDdpik2 null cells were smaller than wild-type cells and grew slowly both in association with bacteria and in axenic medium when attached to petri plates but were unable to grow in suspension in axenic medium. When ΔDdpik1 ΔDdpik2 null cells were plated for multicellular development, they formed aggregates having multiple tips and produced abnormal fruiting bodies. Antisense expression of DdPIK5 (a putative homolog of the Saccharomyces cerevisiae VPS34) led to a defect in the growth of D. discodeum cells on bacterial lawns and abnormal development. DdPIK5 complemented the temperature-sensitive growth defect of a Schizosaccharomyces pombe ΔSvps34 mutant strain, suggesting DdPIK5 encodes a functional homolog of yeast Vps34p. These observations indicate that in D. discoideum, different PI kinases regulate distinct cellular processes, including cell growth, development, and protein trafficking.

    DOI: 10.1128/MCB.15.10.5645

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  • A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum : biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development. Reviewed International journal

    Zhou K, Takegawa K, Emr SD and Firtel RA

    Molecular and Cellular Biology   1995.8

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    DOI: doi: 10.1128/MCB.15.10.5645.

  • Enhanced transglycosylation activity of Arthrobacter protophormiae endo-β-N-acetylglucosaminidase in media containing organic solvents Reviewed

    Fan J.Q., Takegawa K., Iwahara S., Kondo A., Kato I., Abeygunawardana C., Lee Y.C.

    Journal of Biological Chemistry   270 ( 30 )   17723 - 17729   1995.7   ISSN:00219258

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    The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (endo-A) was enhanced by inclusion of organic solvents in the reaction mixture. In aqueous solution, the transglycosylation yield relative to starting substrate was 32% using Man9GlcNAc2Asn as donor and 0.5 M GlcNAc as acceptor. However, in the media containing 30% (v/v) acetone, dioxane, N,N-dimethylformamide, or dimethyl sulfoxide with 0.5 M GlcNAc as acceptor, the transglycosylation attained yields of 89, 13, 28, and 75%, respectively, as analyzed by high performance anion exchange chromatography. The enzyme was stable in media containing up to 30% acetone, 30% dimethyl sulfoxide, or 20% N,N-dimethylformainide at 37 °C for at least 30 min. The acceptor (GlcNAc) concentration must be greater than 0.2 M for efficient transglycosylation. Electrospray mass spectrometry analysis of the transglycosylation product obtained in 30% acetone with Man5GlcNAc2Asn as donor and methyl α-2-acetamido-2-deoxy-D-glucopyranoside as acceptor showed a mass ion of m/z 1249.4, consistent with the expected molecular weight. Analysis by 1H NMR of the product revealed that transglycosylation occurred at the C-4 of GlcNAc and the linkage was of the β-configuration. In the acetone-containing medium, Glc, Man, 2-deoxy-Glc, and methyl α-D-GlcNAc can serve as a good acceptor as GlcNAc. Less favorable acceptors are xylose, fructose, 6-deoxy-Glc, and 3-O-methyl-D-glucose. On the other hand, GalNAc, Gal, allose, and 3-deoxyGlc could not serve as acceptors of the enzyme transglycosylation.

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  • Enhanced transglycosylation activity of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in media containing organic solvents. Reviewed International journal

    Fan J-Q, Takegawa K, Iwahara S, Kondo A, Kato I, Abeygunawardana C and Lee YC

    The Journal of Biological Chemistry   1995.7

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    DOI: doi: 10.1074/jbc.270.30.17723.

  • Synthesis of neoglycoconjugates by transglycosylation with Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase : Demonstration of a macro-cluster effect for mannose-binding proteins. Reviewed International journal

    Fan J-Q, Quesenberry MS, Takegawa K, Iwahara S, Kondo A, Kato I and Lee YC

    The Journal of Biological Chemistry   1995.7

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    DOI: doi: 10.1074/jbc.270.30.17730.

  • Isolation and characterization of novel endo-beta-galactofuranosidase from Bacillus sp. Reviewed

    Ramli N, Fujinaga M, Tabuchi M, Takegawa K and Iwahara S

    Bioscience, Biotechnology, and Biochemistry   1995.6

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    DOI: doi: 10.1271/bbb.59.1856.

  • Degradation of beta1-6galactofuranoside linkages in the polysaccharide of Fusarium sp. M7-1 by endo-beta-galactofuranosidase from Bacillus sp. Invited Reviewed International journal

    Iwahara S, Ramli N and Takegawa K

    Bioscience, Biotechnology, and Biochemistry   1995.5

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    DOI: doi: 10.1271/bbb.60.957.

  • Vesicle-mediated protein transport: Regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. Reviewed International journal

    Stack JH, DeWald DB, Takegawa K and Emr SD

    Journal of Cell Biology   1995.5

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    DOI: doi: 10.1083/jcb.129.2.321.

  • Isolation and identification of novel acidic oligosaccharides derived from glycoproteins of Fusarium sp. M7-1. Reviewed

    Iwahara S, Suemori N, Ramli N, and Takegawa K

    Bioscience, Biotechnology, and Biochemistry   1995.3

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    DOI: doi: 10.1271/bbb.59.1082.

  • Synthesis of neoglycoproteins using oligosaccharide-transfer activity with endo-beta-N-acetylglucosaminidase. Reviewed International journal

    Takegawa K, Tabuchi M, Yamaguchi S, Kondo A, Kato I and Iwahara S

    The Journal of Biological Chemistry   1995.2

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    DOI: doi: 10.1074/jbc.270.7.3094.

  • Isolation and Characterization of a Novel Endo-β-galactofuranosidase from Bacillus sp Reviewed

    Ramli N., Fujinaga M., Tabuchi M., Takegawa K., Iwahara S.

    Bioscience, Biotechnology, and Biochemistry   59 ( 10 )   1856 - 1860   1995   ISSN:09168451

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    A soil bacterium capable of growing on a polysaccharide containing β(1→6)galactofuranoside residues derived from the acidic polysaccharide of Fusarium sp. as a carbon source has been isolated. From various bacteriological characteristics, the organism was identified as a Bacillus sp. The bacterium produced β-galactofuranosidase inductively in the culture media. The most effective inducer for the β-galacto-furanosidase production was a polysaccharide containing β(1→5) or β(1→6)-linked galactofuranoside residues, but gum arabic, gum guar, gum ghati, arabinogalactam, araban, and pectic acid did not induce the enzyme. The enzyme had three different molecular weight forms. The low molecular-weight form was purified by a combination of Toyopearl HW-55 and DEAE-Toyopearl 650S column chromatographies, and preparative polyacrylamide gel electrophoresis. The molecular weight of the enzyme was estimated to be 67,000 by SDS-polyacrylamide gel electrophoresis. The enzyme was most active at pH 6 and 37°C, and was stable between pH 4 to 8 at 5oC. The action of the enzyme was inhibited by the addition of Cd2+, Co2+, Hg2+, Zn2+, iodoacetic acid, and EDTA. The purified enzyme cleaved β(1→5) and β(1→6)-linked galactofuranosyl chains. Based upon the mode of liberation of galactofuranosyl residues from pyridylamino-β(1→6)-linked galactofuranoside oligomers, the enzyme can be classified as an endo-β-galactofuranosidase that randomly hydrolyzes the linkage. © 1995, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb.59.1856

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  • Isolation and Identification of Novel Acidic Oligosaccharides Derived from Glycoproteins of Fusarium Sp. M7-1 Reviewed

    Iwahara S., Suemori N., Ramli N., Takegawa K.

    Bioscience, Biotechnology, and Biochemistry   59 ( 6 )   1082 - 1085   1995   ISSN:09168451

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    Four novel oligosaccharide chains were isolated from the glycoproteins of Fusarium sp. M7-1. Their chemical structures were resolved mainly by 400 MHz NMR spectrometry and mass spectrometry. The following structures were identified. Man α1→2Man-ol-6-P, Manα1→2Manα1→2Man-ol-6-P, Rhaα1→2Manα1→2Manα1→2Man-ol-6-P, and GlcNAcα1→4GlcAα1→2(GlcNAcα1→4)GlcAα1→2Galfβ1→6(Rhaα1→2)Man-ol. © 1995, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb.59.1082

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  • AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain Reviewed

    Welters P., Takegawa K., Emr S.D., Chrispeels M.J.

    Proceedings of the National Academy of Sciences of the United States of America   91 ( 24 )   11398 - 11402   1994.11   ISSN:00278424

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    The cDNA encoding phosphatidylinositol (PI) 3-kinase was cloned from Arabidopsis thaliana, and the derived amino acid sequence (AtVPS34) has a significantly higher homology to yeast PI 3-kinase (VPS34) than to the mammalian (p110). The protein has two conserved domains: a catalytic site with the ATP-binding site near the C terminus and a calcium-dependent lipid- binding domain near the N terminus. The plant cDNA does not rescue a yeast vps34 deletion mutant, but a chimeric gene in which the coding sequence for the C-terminal third of VPS34 is replaced by the corresponding sequence from the plant gene does rescue the yeast mutant. PI 3-kinase activity is detectable in extracts from plants that overexpress the plant PI 3-kinase. Expression of antisense constructs gives rise to second-generation transformed plants severely inhibited in growth and development.

    DOI: 10.1073/pnas.91.24.11398

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  • Preparation of beta (1-6)-linked galactofuranoside oligomers from the acidic polysaccharide of Fusarium sp. M7-1. Reviewed

    Ramli N, Shinohara H, Takegawa K and Iwahara S

    Journal of Fermentation and Bioengineering   1994.7

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  • Degradation of unsaturated polysaccharide derived from acidic polysaccharide of Fusarium sp. M7-1 by a bacterium isolated from soil. Reviewed

    Ramli N, Takegawa K and Iwahara S

    Journal of Fermentation and Bioengineering   1994.5

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  • AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana is an essential protein with homology to a calcium-dependent lipid binding domain. Reviewed International journal

    Welters P, Takegawa K, Emr S and Chrispeels MJ

    The Proceedings of the National Academy of Sciences   1994.5

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    DOI: doi: 10.1073/pnas.91.24.11398.

  • Preparation of β(1→6)-linked galactofuranoside oligomers from the acidic polysaccharide of Fusarium sp. M7-1 Reviewed

    Ramli N., Shinohara H., Takegawa K., Iwahara S.

    Journal of Fermentation and Bioengineering   78 ( 5 )   341 - 345   1994   ISSN:0922338X

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    This paper describes a method of preparating β(1→6)-linked galactofuranoside oligomers. The main structure of the acidic polysaccharide of Fusarium sp. M7-1 has a linear chain composed of β(1→6)-linked galactofuranose residues. For preparation of the β(1→6) galactofuranoside oligomers, glucuronic acid residues were converted to unsaturated sugars using an acidic polysaccharide lyase from Cellulomonas sp. Mild acid hydrolysis was found to be very effective in preparing the β(1→6) galactofuranoside oligomers from the unsaturated polysaccharide of Fusarium sp. M7-1. After treatment with 1 M acetic acid at 100°C for 5 h, the unsaturated polysaccharide was separated into oligomers, and the primary structures of the β(1→6) galactofuranoside oligomers were resolved, mainly by 400-MHz 1H-NMR spectrometry. These oligomers were resistant to commercial β-d-galactosidases. © 1994.

    DOI: 10.1016/0922-338X(94)90277-1

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  • Degradation of unsaturated polysaccharide derived from acidic polysaccharide of Fusarium sp. M7-1 by a bacterium isolated from soil Reviewed

    Ramli N., Takegawa K., Iwahara S.

    Journal of Fermentation and Bioengineering   77 ( 5 )   572 - 574   1994   ISSN:0922338X

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    A soil bacterium, strain no. 19, capable of using unsaturated polysaccharide derived from acidic polysaccharide of Fusarium sp. M7-1 as a sole source of carbon was isolated. The bacterium degraded about 70% of the total sugar content. Results from analysis of the degraded polysaccharide showed that the bacterium degraded the β(1→6) galactofuranoside linkage as well as the unsaturated glucuronic acid residues linked to the galactofuranoside residues via the α(1→2) linkage. © 1994.

    DOI: 10.1016/0922-338X(94)90133-3

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  • Isolation and identification of ethyl--N-acetylglucosaminide from yeast extract. Reviewed

    1993.7

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  • The presence of trehalose-containing oligosaccharides in yeast extract. Reviewed

    Iwahara S, Takegawa K, Kawaguchi K and Okamoto G

    Bioscience, Biotechnology, and Biochemistry   1993.5

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    DOI: doi: 10.1271/bbb.57.1220.

  • Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Reviewed International journal

    Schu PV, Takegawa K, Fry MJ, Stack JH, Waterfield MD and Emr SD

    Science   1993.4

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    DOI: doi: 10.1126/science.8385367.

  • Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting Reviewed

    Schu P.V., Takegawa K., Fry M.J., Stack J.H., Waterfield M.D., Emr S.D.

    Science   260 ( 5104 )   88 - 91   1993   ISSN:00368075

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    The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.

    DOI: 10.1126/science.8385367

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  • Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: IV. Isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain. Reviewed

    Iwahara S, Maeyama T, Mishima T, Jikibara T, Takegawa K and Iwamoto H

    Journal of Biochemistry   1992.7

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    DOI: 10.1093/oxfordjournals.jbchem.a123905.

  • Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins. Reviewed

    Jikibara T, Tada K, Takegawa K and Iwahara S

    Journal of Biochemistry   1992.3

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    DOI: 10.1093/oxfordjournals.jbchem.a123742.

  • Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: III. The primary structures of the acidic polysaccharides of the glycoproteins. Reviewed

    Jikibara T, Takegawa K and Iwahara S

    Journal of Biochemistry   1992.3

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    DOI: 10.1093/oxfordjournals.jbchem.a123743.

  • Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: I. Isolation and some properties of the glycoproteins. Reviewed

    Jikibara T, Takegawa K and Iwahara S

    Journal of Biochemistry   1992.1

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    DOI: 10.1093/oxfordjournals.jbchem.a123741

  • Isolation of two endo-β-N-acetylglucosaminidases with different specificities from Pseudomonas sp Reviewed

    Takegawa K., Naitoh T., Iwahara S.

    Biochemistry International   24 ( 5 )   793 - 799   1991.12   ISSN:01585231

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    Two endo-β-N-acetylglucosaminidases (PI and PII) have been isolated from the culture fluid of Pseudomonas sp. The substrate specificity of the PI enzyme was very similar to that of Endo-H from Streptomyces plicatus. On the contrary, the PII enzyme had a novel substrate specificity that degraded both high-mannose type and hybrid type oligosaccharides derived from ovalbumin, and the core structure of complex type oligosaccharides derived from human transferrin and porcine pancreatic lipase.

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  • Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger Reviewed

    Takegawa K., Konjo A., Iwamoto H., Fujiwara K., Hosokawa Y., Kato I., Hiromi K., Iwahara S.

    Biochemistry International   25 ( 1 )   181 - 190   1991.12   ISSN:01585231

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    The primary structure of the N-linked sugar chains of glucose oxidase from Aspergillus niger was investigated. These sugar chains were released from the polypeptide backbone by hydrazinolysis, and the reducing ends of the sugar chains were pyridylaminated. HPLC of the pyridylamino sugar chains with an amide-silica column showed at least seven sugar chain peaks. Chemical and exoglycosidase, digestion and 400MHz 1H-NMR studies of the sugar chains of lower molecular weight showed that these were novel oligomannose-type sugar chains, (Man)5-7(GlcNAc)2, with the structure: ±Manα1→3Manα1→3(Manα1→6)Manα1→6 (±Manα1→3Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc.

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  • Determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-β-N-acetylglucosaminidase Reviewed

    Takegawa K., Yoshikawa M., Mishima T., Nakoshi M., Iwahara S.

    Biochemistry International   25 ( 3 )   585 - 592   1991.12   ISSN:01585231

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    Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was tested for its capacity to release N-linked sugar chains from native yeast invertase. The enzyme liberated about 80% of the sugar chains from the native invertase. Deglycosylated invertase was digested by chymotrypsin or pepsin, and twelve N-acetylglucosamine-containing glycopeptides were isolated. The amino acid sequences of these glycopeptides were analyzed by a protein sequencer, and the elution position of 4-L-aspartylglycosylamine was directly identified by conventional sequencing. The endo-β-N-acetylglucosaminidase was found to remove mainly nine sugar chains from native invertase.

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  • Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae Reviewed

    Takegawa K., Yamaguchi S., Kondo A., Kato I., Iwahara S.

    Biochemistry International   25 ( 5 )   829 - 835   1991.12   ISSN:01585231

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    The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

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  • Complete amino acid sequence of endo‐β‐N‐acetylglucosaminidase from Flavobacterium sp. Reviewed

    TAKEGAWA K., MIKAMI B., IWAHARA S., MORITA Y., YAMAMOTO K., TOCHIKURA T.

    European Journal of Biochemistry   202 ( 1 )   175 - 180   1991.11   ISSN:00142956

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    The complete amino acid sequence of endo‐β‐N‐acetylglucosaminidase from Flavobacterium sp. has been determined by analysis of peptides after cleavage with lysyl endopeptidase, pepsin and chymotrypsin. The protein consists of a single polypeptide chain consisting of 267 amino acid residues and a molecular mass of 27972 Da. The sequence of Flavobacterium endo‐β‐N‐acetylglucosaminidase is very close to that of the Streptomyces enzyme (endo‐H), having 60% similarity and very similar hydropathy profiles. Similarities were also found between Flavobacterium endo‐β‐N‐acetylglucosaminidase and chitinases from Bacillus circulans, Serratia marcescens and Phaseolus vulgaris. Copyright © 1991, Wiley Blackwell. All rights reserved

    DOI: 10.1111/j.1432-1033.1991.tb16359.x

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  • Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae. Reviewed International journal

    Takegawa K, Yamaguchi S, Kondo A, Kato I and Iwahara S

    Biochemistry International   1991.7

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  • Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae. Reviewed International journal

    Takegawa K, Yamaguchi S, Kondo A, Iwamoto H, Nakoshi M, Kato I and Iwahara S

    Biochemistry International   1991.6

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  • Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger. Reviewed International journal

    Takegawa K, Kondo A, Iwamoto H, Fujiwara K, Hosokawa Y, Kato I, Hiromi K and Iwahara S

    Biochemistry International   1991.6

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  • Determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-N-acetylglucosaminidase. Reviewed International journal

    Takegawa K, Yoshikawa M, Mishima T, Nakoshi M and Iwahara S

    Biochemistry International   1991.6

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  • Complete amino acid sequence of endo-beta-N-acetylglucosaminidase from Flavobacterium sp. Reviewed International journal

    Takegawa K, Mikami B, Iwahara S, Morita Y, Yamamoto K and Tochikura T

    Europian Journal of Biochemistry   1991.5

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    DOI: 10.1111/j.1432-1033.1991.tb16359.x.

  • Isolation of two endo-beta-N-acetylglucosaminidases with different specificities from Pseudomonas sp. Reviewed International journal

    Takegawa K, Naitoh T and Iwahara S

    Biochemistry International   1991.5

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  • Purification and properties of chondroitinase produced by a bacterium isolated from soil. Reviewed International journal

    Takegawa K, Iwahara K and Iwahara S

    Journal of Fermentation and Bioengineering   1991.4

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  • Purification and characterization of a novel lyase from Cellulomonas sp. that degrades Fusarium and Gibberella acidic polysaccharides. Reviewed

    Takegawa K, Yamaguchi S, Miki S, Jikibara T and Iwahara S

    Agricultural and Biological Chemistry   1991.3

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  • Primary structure of an O-linked sugar chain derived from glucose oxidase of Aspergillus niger Reviewed

    Takegawa K., Fujiwara K., Iwahara S., Yamamoto K., Tochikura T.

    Agricultural and Biological Chemistry   55 ( 3 )   883 - 884   1991.3   ISSN:00021369

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    DOI: 10.1080/00021369.1991.10870652

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  • Activation of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae by various sugars. Reviewed

    Takegawa K, Nakoshi M, Yamamoto K, Tochikura T and Iwahara S

    Journal of Fermentation and Bioengineering   1991.2

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  • Primary structure of an O-linked sugar chain derived from glucose oxidase of Aspergillus niger. Reviewed

    Takegawa K, Fujiwara K, Iwahara S, Yamamoto K and Tochikura T

    Agricultural and Biological Chemistry   1991.1

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  • Purification and properties of chondroitinase produced by a bacterium isolated from soil Reviewed

    Takegawa K., Iwahara K., Iwahara S.

    Journal of Fermentation and Bioengineering   72 ( 2 )   128 - 131   1991   ISSN:0922338X

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    A Gram-positive bacterium isolated from a soil sample capable of growing on chondroitin sulfate as a carbon source has been isolated. From various bacteriological characteristics, the organism was identified as either an Aureobacterium or a Curtobacterium. When grown on chondroitin sulfate, the bacterium secreted a chondroitinase (EC 4.2.2.4 or EC 4.2.2.5) into the culture medium. The enzyme was purified to homogeneity as demonstrated by polyacrylamide gel electrophoresis and has an apparent molecular weight of about 81,000. It was inhibited by Hg2+, Zn2+, Fe2+ and Cu2+ ions. The enzyme hydrolyzed chondroitin sulfate-A, -C, chondroitin and hyaluronic acid, although the hydrolysis rate for hyaluronic acid was low. On the other hand, the enzyme did not act on iduronic acid-containing mucopolysaccharides such as dermatan sulfate, heparin and heparan sulfate. A decrease in viscosity and gel filtration analyses showed that the purified enzyme degrades the substrate endolytically at the initial reaction. © 1991.

    DOI: 10.1016/0922-338X(91)90323-9

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  • Purification and Characterization of a Novel Lyase from Cellulomonas sp. that Degrades Fusarium and Gibberella Acidic Polysaccharides Reviewed

    Takegawa K., Yamaguchi S., Miki S., Jikibara T., Iwahara S.

    Agricultural and Biological Chemistry   55 ( 8 )   1969 - 1975   1991   ISSN:00021369

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    A Cellulomonas sp. isolated from soil produced a novel lyase that degraded the acidic polysaccharide of Fusarium sp. M7-1 with the formation of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. DEAE-Toyopearl 650M column chromatography showed three lyase activity peaks (fractions I, II, and III). The major fraction was purified to homogeneity by polyacrylamide gel electrophoresis analysis, and its molecular weight was 74,000. The optimum pH was 6.5 to 8.0 and the stable pH range was 6.0 to 8.0. The purified enzyme did not degrade glucuronic or galacturonic acid-containing polysaccharides such as chondroitin, hyaluronic acid, pectin, or pectic acid. However, the purified enzyme specifically degraded various Fusarium and Gibberella acidic polysaccharides, and unsaturated sugars were produced with the release of mannose and O-β-D-mannopyranosyl-(1 → 2)-D-mannose. These results suggest that the acidic polysaccharides derived from Fusarium and Gibberella have similar structures. © 1991, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb1961.55.1969

    Scopus

  • Primary Structure of an 0-Linked Sugar Chain Derived from Glucose Oxidase of Aspergillus niger Reviewed

    Takegawa K., Fujiwara K., Iwahara S., Yamamoto K., Tochikura T.

    Agricultural and Biological Chemistry   55 ( 3 )   883 - 884   1991   ISSN:00021369

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    DOI: 10.1271/bbb1961.55.883

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  • Activation of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae by various sugars Reviewed

    Takegawa K., Nakoshi M., Yamamoto K., Tochikura T., Iwahara S.

    Journal of Fermentation and Bioengineering   71 ( 4 )   278 - 280   1991   ISSN:0922338X

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    Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was activated by the addition of glucose, mannose, N-acetylglucosamine, and β-allose. While the enzyme did not appear to be significantly affected by the addition of galactose or N-acetylgalactosamine. These results indicate that the C-4 and C-6 positions of the monosaccharide are the most important for enzyme activation. Moreover, the enzyme was activated by the addition of disaccharides such as cellobiose, gentiobiose, and di-N-acetylchitobiose, but not by polysaccharides such as starch and yeast mannan. In the presence of N-acetylglucosamine, the enzyme activation occurred well over pH 4.0 and the Km value of the enzyme for (Man)6(GlcNAc)2-Asn-dansyl changes from 1.2 mM to 3.2 mM. © 1991.

    DOI: 10.1016/0922-338X(91)90282-L

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  • Effect of deglycosylation of polymannose chains on the properties of yeast external invertase. Reviewed

    Takegawa K, Miki S and Iwahara S

    Journal of Fermentation and Bioengineering   1990.8

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  • Identification of O-beta-D-glucopyranosyluronate-(1-2)-D-galactose isolated from the acidic polysaccharide of Fusarium sp. M7-1. Reviewed International journal

    Iwahara S, Jikibara T and Takegawa K

    Agricultural and Biological Chemistry   1990.6

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Purification and properties of low-molecular-weight alpha-mannosidase from Cellulomonas sp. Reviewed International journal

    Takegawa K, Miki S, Jikibara T and Iwahara S

    Journal of Fermentation and Bioengineering   1990.3

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  • Beta-eliminative cleavage of the acidic polysaccharide of Fusarium sp. M7-1 by an enzyme preparation of Cellulomonas sp. Reviewed International journal

    Iwahara S, Jikibara T, Miki S and Takegawa K

    Agricultural and Biological Chemistry   1990.1

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  • Purification and properties of a low-molecular-weight α-mannosidase from Cellulomonas sp. Reviewed

    Takegawa K., Miki S., Jikibara T., Iwahara S.

    Journal of Fermentation and Bioengineering   69 ( 2 )   129 - 131   1990   ISSN:0922338X

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    Cellulomonas sp. isolated from soil produces a high level of α-mannosidase (α-mannanase) inductively in culture fluid. The enzyme had two different molecular weight forms, and the properties of the high-molecular-weight form were reported previously (Takegawa, K. et al.: Biochim. Biophys. Acta, 991, 431-437, 1989). The low-molecular-weight α-mannosidase was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was over 150,000 by gel filtration. Unlike the high-molecular-weight form, the low-molecular-weight enzyme readily hydrolyzed α-1,2- and α-1,3-linked mannose chains. © 1990.

    DOI: 10.1016/0922-338X(90)90201-7

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  • Effect of deglycosylation of polymannose chains on the properties of yeast external invertase Reviewed

    Takegawa K., Miki S., Iwahara S.

    Journal of Fermentation and Bioengineering   70 ( 2 )   131 - 133   1990   ISSN:0922338X

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    α-Mannosidase from Cellulomonas sp. released about 70% of the polymannose chains from yeast external invertase. Native and mannose-depleted yeast invertases were compared with regard to various enzymatic properties. It was found that their catalytic activity and thermal and pH stability were identical. However, the mannose-depleted invertase was more sensitive to proteinases such as pronase and subtilisin. The mannose-depleted invertase was less stable than the native enzyme when incubated with sodium dodecyl sulfate. These results show that the polymannose chains of yeast invertase contribute to the high stability of the enzyme. © 1990.

    DOI: 10.1016/0922-338X(90)90285-5

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  • Identification of O-α-D-Glucopyranosyluronate-( 1 →2)- D -galactose Isolated from the Acidic Polysaccharide of Fusarium sp. M7-1 Reviewed

    Iwahara S., Jikibara T., Takegawa K.

    Agricultural and Biological Chemistry   54 ( 6 )   1559 - 1561   1990   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    DOI: 10.1271/bbb1961.54.1559

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  • Induction and purification of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin. Reviewed International journal

    Takegawa K, Nakoshi M, Iwahara S, Yamamoto K and Tochikura T

    Applied and Environmental Microbiology   1989.10

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    DOI: 10.1128/aem.55.12.3107-3112.1989.

  • Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger. Reviewed

    Takegawa K., Fujiwara K., Iwahara S., Yamamoto K., Tochikura T.

    Biochemistry and cell biology = Biochimie et biologie cellulaire   67 ( 8 )   460 - 464   1989.8   ISSN:08298211

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    Endo-beta-N-acetylglucosaminidase from Flavobacterium sp. released about 30% of the N-linked sugar chains from the glucose oxidase of Aspergillus niger. To elucidate the role of the carbohydrate moiety, the enzymatic properties of native and carbohydrate-depleted glucose oxidases were compared. It was found that their catalytic activities and thermal and pH stabilities were identical. However, the carbohydrate-depleted glucose oxidase was more rapidly precipitated by the addition of trichloroacetic acid and ammonium sulfate than the native enzyme. These results show that the N-linked sugar chains of the glucose oxidase contributed to the high solubility of the enzyme in water.

    DOI: 10.1139/o89-072

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  • Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger. Reviewed International journal

    Takegawa K, Fujiwara K, Iwahara S, Yamamoto K and Tochikura T

    Biochemistry and Cell Biology   1989.7

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    DOI: 10.1139/o89-072.

  • Purification and characterization of exo-α-d-mannosidase from a Cellulomonas sp. Reviewed

    Takegawa K., Miki S., Jikibara T., Iwahara S.

    BBA - General Subjects   991 ( 3 )   431 - 437   1989.6   ISSN:03044165

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    A Gram-positive bacterium isolated from a soil sample was found to produce a high level of α-mannosidase in culture media. The organism was identified as Cellulomonas sp. from various bacteriological characteristics. The production of the enzyme was strongly induced by yeast extract. To purify the enzyme, the bacterium was first grown in a glucose medium, and then the cells were transferred to an inducing medium containing only yeast extract. The enzyme had two different molecular weight forms. The high-molecular-weight form was purified from the inducing medium by column chromatography. The enzyme was purified to homogeneity by ultracentrifugal analysis. The enzyme was strongly inactivated by Hg2+, Cu2+, Zn2+, EDTA and p-chloromercuribenzoic acid. The molecular weight of the enzyme was estimated to be about 450 000 by gel filtration. The purified enzyme could liberate only mannose from native yeast mannan and the optimum pH was 6.5-8.0. The enzyme rapidly cleaved α-1,2- and α-1,6-linked mannose chains, but the hydrolysis rate for α-1,3 linkage was very low. In addition, the purified enzyme showed only slight activity towards p-nitrophenyl-α-d-mannoside and did not hydrolyze O-α-d-mannopyranosyl(1 → 2)-d-mannitol. © 1989.

    DOI: 10.1016/0304-4165(89)90069-X

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  • Action of beta-mannosidases on O--D-mannopyranosyl-(1-2)-D-mannose. Invited Reviewed

    1989.5

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  • Action of β-mannosidases on o-β-D-mannopyranosyl-(L→2)-D-mannose Reviewed

    Takegawa K., Miki S., Osaka F., Jikibara T., Iwahara S.

    Agricultural and Biological Chemistry   53 ( 4 )   1179 - 1180   1989.4   ISSN:00021369

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    DOI: 10.1080/00021369.1989.10869413

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  • Purification and characterization of exo-alpha-D-mannosidase from a Cellulomonas sp. Reviewed International journal

    Takegawa K, Miki S, Jikibara T and Iwahara S

    Biochimica et Biophysica Acta   1989.3

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  • Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus. Reviewed International journal

    Takegawa K, Kawasaki N, Iwahara S, Yamamoto K, Tochikura T, Mikami B and Morita Y

    Biochimica et Biophysica Acta   1989.1

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    DOI: 10.1016/s0304-4165(89)80018-2.

  • Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus Reviewed

    Takegawa K., Kawasaki N., Iwahara S., Yamamoto K., Tochikura T., Mikami B., Morita Y.

    Biochimica et Biophysica Acta - General Subjects   990 ( 1 )   98 - 100   1989   ISSN:03044165

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    The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-β-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNAc-ol. © 1989, Elsevier Science Publishers B.V. (Biomedical Division). All rights reserved.

    DOI: 10.1016/S0304-4165(89)80018-2

    Scopus

  • Action of β-Mannosidases on O-β-D-Mannopyranosyl-(1-&gt;2)-D-mannose Reviewed

    Takegawa K., Miki S., Osaka F., Jikibara T., Iwahara S.

    Agricultural and Biological Chemistry   53 ( 4 )   1179 - 1180   1989   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    DOI: 10.1271/bbb1961.53.1179

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  • Induction and purification of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin Reviewed

    Takegawa K., Nakoshi M., Iwahara S., Yamamoto K., Tochikura T.

    Applied and Environmental Microbiology   55 ( 12 )   3107 - 3112   1989   ISSN:00992240

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    Arthrobacter protophormiae produced a high level of extracellular endo-β-N-acetylglucosaminidase when cells were grown in a medium containing ovalbumin. The enzyme was incuded by the glycopeptide fraction of ovalbumin prepared by pronase digestion. Production of the enzyme was also induced by glycoproteins such as yeast invertase and bovine ribonuclease B but not by monosaccharides such as mannose, N-acetylglucosamine, and galactose. The enzyme was purified to homogeneity as demonstrated by polyacrylamide gel electrophoresis and has an apparent molecular weight of about 80,000. The enzyme showed a broad optimum pH in the range of pH 5.0 to 11.0. The enzyme hydrolyzed all heterogeneous ovalbumin glycopeptides, although the hydrolysis rates for hybrid type glycopeptides were very low. The substrate specificity of A. protophormiae endo-β-N-acetylglucosaminidase was very similar to that of Endo-C(II) from Clostridium perfringens. Therefore, the enzyme induction by A. protophormiae seems to have a close relation to the substrate specificity of the enzyme.

    DOI: 10.1128/aem.55.12.3107-3112.1989

    Scopus

  • Deglycosylated glucoamylase from rhizopus niveus is precipitated by flavobacterium sp. Endo-β- n-acetylglucosaminidase Reviewed

    Takegawa K., Inami M., Yamamoto K., Kumagai H., Tochikura T., Mikami B., Morita Y.

    Agricultural and Biological Chemistry   52 ( 11 )   2941 - 4942   1988.11   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    DOI: 10.1080/00021369.1988.10869167

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  • Deglycosylated glucoamylase from Rhizopus niveus is precipitated by Flavobacterium sp. endo-beta-N-acetylglucosaminidase. Reviewed

    Takegawa K, Inami M, Yamamoto K, Kumagai H, Tochikura T, Mikami B and Morita Y

    Agricultural and Biological Chemistry   1988.9

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  • Elucidation of the role of sugar chains in glucoamylase using endo-β-N-acetylglucosaminidase from Flavobacterium sp. Reviewed

    Takegawa K., Inami M., Yamamoto K., Kumagai H., Tochikura T., Mikami B., Morita Y.

    Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular   955 ( 2 )   187 - 193   1988.7   ISSN:01674838

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    Language:English   Publisher:Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular  

    Endo-β-N-acetylglucosaminidase (glycopeptide-d-mannosyl-N4-(N-acetyl-d-glucosaminyl)2-asparagine1,4-N-acetyl-β-glucosaminohydrolase, EC 3.2.1.96), homogenized from the culture filtrate of Flavobacterium sp., could liberate about 50% of the sugar chains from the glucoamylase of Rhizopus niveus. The native and carbohydrate-depleted glucoamylases were compared in their various enzymatic properties. It was found that they were identical in their catalytic activities. However, the carbohydrate-depleted glucoamylase was less thermally stable than the native glucoamylase. Moreover, the carbohydrate-depleted glucoamylase was more sensitive to proteinases such as pronase, subtilisin and trypsin. These results suggest that the sugar chains of the glucoamylase contribute to the high stability of the enzyme. However, circular dichroism spectra of the native and carbohydrate-depleted glucoamylase were found to be identical. © 1988.

    DOI: 10.1016/0167-4838(88)90192-6

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  • Elucidation of the role of sugar chains in glucoamylase using endo-beta-N-acetylglucosaminidase from Flavobacterium sp. Reviewed International journal

    Takegawa K, Inami M, Yamamoto K, Kumagai H, Tochikura T, Mikami B and Morita Y

    Biochimica et Biophysica Acta   1988.7

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  • Effect of yeast extract on endo--N-acetylglucosaminidase production by a Flavobacterium sp. Reviewed

    1988.3

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  • Deglycosylated Glucoamylase from Rhizopus niveus is Precipitated by Flavobacterium sp. Endo-β-N-acetylglucosaminidase Reviewed

    Takegawa K., Kumagai H., Inami M., Yamamoto K., Tochikura T., Mikami B., Morita Y.

    Agricultural and Biological Chemistry   52 ( 11 )   2941 - 2942   1988   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    DOI: 10.1271/bbb1961.52.2941

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  • Effect of Yeast Extract on Endo-β-N-acetylglucosaminidase Production by a Flavobacterium sp Reviewed

    Kadowaki S., Takegawa K., Yamamoto K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   52 ( 8 )   2105 - 2106   1988   ISSN:00021369

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    DOI: 10.1271/bbb1961.52.2105

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  • Effect of protease digestion on the activity of sugardepleted enzymes prepared with endo-β-N-acetylglucosaminidase from Flavobacterium sp Reviewed

    Yamamoto K., Takegawa K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   51 ( 6 )   1481 - 1487   1987.6   ISSN:00021369

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    Endo-β-N-acetylglucosaminidase, purified to homogeneity from the culture filtrate of a Flavobacterium sp., liberated the carbohydrate chains from yeast invertase. About 90% of the carbohydrate associated with this glycoprotein was removed by the endo-β-N-acetylglucosaminidase. The native and carbohydrate-depleted enzymes were compared and found to exhibit similar catalytic activities, thermal stabilities and pH-activity profiles. However, the carbohydrate-depleted invertase was more susceptible to proteases with relatively broad specificities such as subtilisin and pronase, as found on examination of the enzyme activity and electrophoresis. On the other hand, trypsin did not have such an effect on the enzyme activities of the native and carbohydrate-depleted enzymes. The endo-β-N-acetylglucosaminidase also released carbohydrate chains from the purified β-N-acetylhexosaminidase of Penicillium oxalicum. Although the native and carbohydrate-depleted β-N-acetylhexosaminidases did not differ significantly in their stabilities or pH-activity profiles, the carbohydrate-depleted form was more susceptible to proteolysis by subtilisin, pronase and trypsin. From these results, it would appear that the carbohydrate of a glycosylated enzyme plays a role in protecting the enzyme from proteolysis. © 1987 by the Agricultural Chemical Society of Japan.

    DOI: 10.1080/00021369.1987.10868264

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  • Effect of protease digestion on the activity of sugar-depleted enzymes prepared with endo--N-acetylglucosaminidase from Flavobacterium sp. Reviewed

    1987.1

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  • Effect of Protease Digestion on the Activity of Sugar-depleted Enzymes Prepared with Endo-β-N-acetylglucosaminidase from Flavobacterium sp Reviewed

    Yamamoto K., Takegawa K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   51 ( 6 )   1481 - 1487   1987   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    Endo-β-N-acetylglucosaminidase, purified to homogeneity from the culture filtrate of a Flavobacterium sp., liberated the carbohydrate chains from yeast invertase. About 90% of the carbohydrate associated with this glycoprotein was removed by the The native and carbohydrate-depleted enzymes were compared and found to exhibit similar catalytic activities, thermal stabilities and pH-activity profiles. However, the carbohydrate-depleted invertase was more susceptible to proteases with relatively broad specificities such as subtilisin and pronase, as found on examination of the enzyme activity and electrophoresis. On the other hand, trypsin did not have such an effect on the enzyme activities of the native and carbohydrate-depleted enzymes. The also released carbohydrate chains from the purified of Penicillium oxalicum. Although the native and carbohydrate-depleted did not differ significantly in their stabilities or pH-activity profiles, the carbohydrate-depleted form was more susceptible to proteolysis by subtilisin, pronase and trypsin. From these results, it would appear that the carbohydrate of a glycosylated enzyme plays a role in protecting the enzyme from proteolysis. © 1987, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb1961.51.1481

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  • Elucidation of the role of sugar chains in glycosylated-enzymes using endo-b-n-acetylglu- cosaminidase from flavobacterium sp. Reviewed

    Yamamoto K., Takegawa K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   50 ( 8 )   2167 - 2169   1986.8   ISSN:00021369

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    DOI: 10.1080/00021369.1986.10867721

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  • Elucidation of the role of sugar chains in glycosylated-enzymes using endo--N -acetylglucosaminidase from Flavobacterium sp. Reviewed

    1986.5

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  • The release of oligosaccharides from glycoproteins by endo--N-acetylglucosaminidase of Flavobacterium sp. Reviewed

    1986.3

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  • Purification and characterization of endo-β-iv-acetyl-glucosaminidase from a flavobacterium sp. Reviewed

    Yamamoto K., Kadowaki S., Takegawa1 K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   50 ( 2 )   421 - 429   1986.2   ISSN:00021369

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    A gram negative bacterium isolated from soil was found to produce a high level of endo-fi-N-acetylglucosaminidase in the culture medium. The organism was identified as a Flavobacterium sp. from various bacteriological characteristics. The enzyme from the Flavobacterium sp. was purified to homogeneity from culture broth by fractionation with ammonium sulfate and column chromatographies on DEAE-cellulose, hydroxylapatite, and Sephadex G-150 and G-100. The molecular weight of the enzyme was estimated to be 27, 000 and 30, 000 by gel filtration and SDS-polyacrylamide gel electrophoresis, respectively, and it appeared to consist of a single polypeptide chain. The optimal pH for activity was 5.0 to 6.0 and the stable pH range was 5-7. The Michaelis constant was 0.30 mM with dansyl-Asn-(GlcNAc)2(Man)6 as the substrate. The enzyme hydrolyzed oligosaccharides of native ovalbumin, bovine pancreatic ribonucléase B and a yeast invertase. © 1986 by the Agricultural Chemical Society of Japan.

    DOI: 10.1080/00021369.1986.10867399

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  • Purification and characterization of endo--N-acetylglucosaminidase from a Flavobacterium sp. Reviewed

    1986.1

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  • Elucidation of the Role of Sugar Chains in Glycosylated-enzymes Using Endo-β-N-acetylglucosaminidase from Flavobacterium sp. Reviewed

    Yamamoto K., Takegawa K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   50 ( 8 )   2167 - 2169   1986.1   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    DOI: 10.1271/bbb1961.50.2167

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  • Purification and Characterization of Endo-β-N-acetylglucosaminidase from a Flavobacterium sp Reviewed

    Yamamoto K., Kadowaki S., Takegawa K., Kumagai H., Tochikura T.

    Agricultural and Biological Chemistry   50 ( 2 )   421 - 429   1986   ISSN:00021369

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    Language:English   Publisher:Agricultural and Biological Chemistry  

    A gram negative bacterium isolated from soil was found to produce a high level of Endo-β-N-acetylglucosaminidase in the culture medium. The organism was identified as a Flavobacterium sp. from various bacteriological characteristics. The enzyme from the Flavobacterium sp. was purified to homogeneity from culture broth by fractionation with ammonium sulfate and column chromatographies on DEAE-cellulose, hydroxylapatite, and Sephadex G-150 and G-100. The molecular weight of the enzyme was estimated to be 27,000 and 30,000 by gel filtration and SDS-polyacrylamide gel electrophoresis, respectively, and it appeared to consist of a single polypeptide chain. The optimal pH for activity was 5.0 to 6.0 and the stable pH range was 5~7. The Michaelis constant was 0.30 mM with dansyl-Asn-(GlcNAc)2(Man)6 as the substrate. The enzyme hydrolyzed oligosaccharides of native ovalbumin, bovine pancreatic ribonuclease B and a yeast invertase. © 1986, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

    DOI: 10.1271/bbb1961.50.421

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    Microbial Production: From Genome Design to Cell Engineering  2014.11    ISBN:9784431546078, 4431546065, 9784431546061

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    The fission yeast Schizosaccharomyces pombe is a promising host for production of heterologous proteins. However, the oligosaccharide structures of yeasts, including S. pombe, differ significantly from those of mammalian cells and humans. In S. pombe, galactose residues are transferred to oligosaccharide moieties of glycoproteins by galactosyltransferases in the lumen of the Golgi apparatus. Therefore, UDP-galactose, a substrate for galactosyltransferases, should be synthesized in the cytosol, and transported into the Golgi apparatus by a UDP-galactose transporter. Because S. pombe cannot use galactose as a carbon or energy source, little is known about galactose metabolism in this species. A galactose-assimilating mutant of S. pombe that was able to grow in minimal galactose medium was isolated. Through DNA microarray analysis of gene expression profiles in the wild type and the mutant, three gal genes (gal7 +, gal10 +, and gal1 +) involved in galactose utilization were found to be highly expressed in the mutant. Although galactose residues are not essential for growth of S. pombe, galactosylation of protein is required for maintenance of normal cell shape, tolerance toward various drugs, and nonsexual flocculation. We identified fission yeast gsf2 +, encoding a flocculin that binds galactose residues located on cell-surface glycoconjugates by DNA microarray analysis. S. pombe appears to have a unique galactose-specific recognition system in which Gsf2/flocculin plays an essential role in mediating cell-cell interactions.

    DOI: 10.1007/978-4-431-54607-8_10

    Scopus

  • Microbial Production -From Genome Design to Cell Engineering- Insights into metabolism and the galactose recognition system from microarray analysis in the fission yeast Schizosaccharomyces pombe.

    Kaoru Takegawa, Tomohiko Matsuzawa( Role: Joint author)

    Springer  2014.2 

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    Responsible for pages:p109-118   Language:English   Book type:General book, introductory book for general audience

  • 酵母の生命科学と生物工学 -産業応用から基礎科学へ- 6章 タンパク質の分泌

    竹川 薫, 松沢智彦( Role: Joint author)

    化学同人  2013.8 

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    Responsible for pages:p83-96   Language:Japanese   Book type:Scholarly book

  • 微生物を活用した新世代の有用物質生産技術 第3章 4項オミックス情報を用いた分裂酵母の改変

    竹川 薫、松沢智彦、東田英毅( Role: Joint author)

    シーエムシー出版  2012.9 

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    Responsible for pages:p70-77   Language:Japanese   Book type:Scholarly book

  • バイオ医薬品開発における糖鎖技術 第3編 合成 第8章 エンドAの構造から糖タンパク質合成の最適条件を探る

    竹川 薫、藤田清貴( Role: Joint author)

    シーエムシー出版  2011.9 

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    Language:Japanese   Book type:General book, introductory book for general audience

  • Experimental Glycoscience (Glycochemistry)

    Takegawa, K., and Yamamoto, K.

    Springer  2008.7 

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    Responsible for pages:Endoglycosidases (Glycoproteins). p182-185   Language:English   Book type:Scholarly book

  • 微生物機能を活用した革新的生産技術の最前線 第4章 分裂酵母のミニマムゲノムファクトリー

    浜 祐子、竹川 薫( Role: Joint author)

    シーエムシー出版  2007.9 

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    Responsible for pages:p49-61   Language:Japanese   Book type:Scholarly book

  • 酵母のすべて

    竹川 薫( Role: Joint author)

    シュプリンガージャパン  2007.9 

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    Responsible for pages:第III章 3.10 液胞, p78-83   Language:Japanese   Book type:General book, introductory book for general audience

  • 遺伝子から見た応用微生物学

    竹川 薫( Role: Joint author)

    朝倉書店  2007.7 

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    Responsible for pages:第3章 微生物遺伝学と遺伝子工学, p30-50   Language:Japanese   Book type:Scholarly book

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Presentations

  • 分裂酵母のガラクトース特異的な細胞間認識システムと物質生産への利用 Invited

    竹川 薫

    第20回酵母合同シンポジウム  2012.9 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京都大学   Country:Japan  

  • 分裂酵母の染色体改変技術を利用した異種タンパク質生産法の開発 Invited

    竹川 薫

    日本農芸化学会2012年度大会  2012.3 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京都女子大学   Country:Japan  

  • Biosynthesis and intracellular trafficking of galactose-containing oligosaccharides in fission yeast. Invited

    Takegawa Kaoru

    2011.9 

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    Event date: 2011.9

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母を用いた異種糖タンパク質生産システムの開発 Invited

    竹川 薫

    応用糖質学会近畿支部会  2010.7 

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    Event date: 2010.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:大阪市立大学   Country:Japan  

  • 真核微生物の細胞表層ガラクトース含有糖鎖の生合成機構と生理的役割 Invited

    竹川 薫

    日本セルロース学会大会  2010.7 

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    Event date: 2010.7

    Presentation type:Oral presentation (invited, special)  

    Venue:徳島文理大学   Country:Japan  

  • 分裂酵母のアミノ酸トランスポーターの局在に重要なスフィンゴ糖脂質の機能と細胞内アミノ酸調節における液胞の役割について Invited

    竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • ガラクトース含有糖鎖の微生物における生合成と役割 Invited

    竹川 薫

    第20回糖鎖科学コーンソーシアムシンポジウム  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 真核微生物の糖鎖末端に存在するガラクトースの生理的役割について

    竹川 薫

    第96回日本生化学会大会  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡国際会議場   Country:Japan  

  • Barnesiella 属腸内細菌の ENGase を用いた N-型複合型糖鎖の資化機構解析

    土井 佳奈子、樋口 裕次郎、竹川 薫

    第42回日本糖質学会年会  2023.9 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鳥取   Country:Japan  

  • 分裂酵母の液胞形成に関与するHOPS複合体の機能解析

    植木 慈, 大久保皓平, 竹川 薫

    第60回化学関連支部合同九州大会  2023.7 

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    Event date: 2023.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombeの細胞質局在アミラーゼホモログの機能解析

    板村稜, 吉川莉乃, 竹川 薫

    第60回化学関連支部合同九州大会  2023.7 

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    Event date: 2023.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける Rab7 GTPaseの生理機能解析

    杉本憲亮、竹川 薫、樋口裕次郎

    第28回日本生物工学会九州支部佐賀大会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Aspergillus nidulans における全 β-D–ガラクトフラノシダーゼ遺伝子の探索

    関口 仁、山田久恵、豊田早紀、松永恵美子、竹川 薫

    第28回日本生物工学会九州支部佐賀大会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 油脂酵母Lipomyces starkeyiのガラクトース含有糖鎖の構造および生合成機構の解析

    森島悠輝、高久洋暁、田中 大、大橋貴生、福永嵩大、竹川 薫

    第28回日本生物工学会九州支部佐賀大会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 黄麹菌 Aspergillus oryzaeにおけるグルコアミラーゼmRNA のライブセルイメージング

    守田湧貴、竹川 薫、樋口裕次郎

    第28回日本生物工学会九州支部佐賀大会  2022.12 

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    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Localization of microtubule constituent proteins in Aspergillus oryzae

    Kawatomi K., Morita Y., Takegawa K and Higuchi Y

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Characterization of novel ENGases from B. intestinihominis that hydrolyze complex type N-glycans

    Doi K., Higuchi Y. and Takegawa K.

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Studies on the function of fission yeast GPI-anchored amylase homologues.

    Yoshikawa R., Nakakita S. and Takegawa K

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Physiological analysis of early endosome dynamics in the endocytic pathway of Aspergillus oryzae

    Hatano W., Takegawa K. and Higuchi Y

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of metabolic pathway of rare sugar 5-KF produced by acetic acid bacteria in yeast

    Noyori Y., Sato R., Takaku H., Takeshita K. and Takegawa K

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Application of optogenetics to control intracellular protein localization in Aspergillus oryzae

    Kawanishi K. Takegawa K., Higuchi Y

    JSBBA West 5th Student Forum  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるエンドサイトーシス関連因子AipAと新規相互作用タンパク質の解析

    日浅 怜子、竹川 薫、樋口 裕次郎

    第21回糸状菌分子生物学コンファレンス  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌生細胞におけるグルコアミラーゼmRNAの時空間制御機構

    守田湧貴、竹川 薫、樋口 裕次郎

    第21回糸状菌分子生物学コンファレンス  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母のアミラーゼホモログ遺伝子の機能解析

    板村 稜、吉川莉乃、竹川 薫

    第39回YEAST WORKSHOP  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母細胞表層糖鎖のピルビン酸付加に関与するII型膜貫通タンパク質 Pvg2,Pvg5 の機能解析

    福永嵩大、渡邊真宏、中道優介、森田友岳、竹川 薫

    第39回YEAST WORKSHOP  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:高知県立県民文化ホール   Country:Japan  

  • 油脂酵母 Lipomyces starkeyi の糖鎖合成欠損変異株の取得とその解析

    森島悠輝、高久洋暁、田中大、大橋貴生、福永嵩大、竹川 薫

    第39回YEAST WORKSHOP  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:高知県立県民文化ホール   Country:Japan  

  • 希少糖 5-ケト-D-フルクトースの油脂酵母 Lipomyces starkeyi における代謝経路の解析

    野寄裕暉慧、佐藤里佳子、高久洋暁、竹下 圭、竹川 薫

    第39回YEAST WORKSHOP  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:高知県立県民文化ホール   Country:Japan  

  • Barnesiella 属腸内細菌のエンドグリコシダーゼの諸性質に関する研究

    土井佳奈子、篠田あかり、中山二郎、樋口裕次郎、竹川 薫

    第41回日本糖質学会年会  2022.9 

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    Event date: 2022.9 - 2022.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:大阪大学吹田キャンパス   Country:Japan  

  • 分裂酵母における β1,3-ガラクトース転移酵素の機能解析

    福永嵩大、渡邊真宏、中道優介、森田友岳、竹川 薫

    第41回日本糖質学会年会  2022.9 

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    Event date: 2022.9 - 2022.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:大阪大学吹田キャンパス   Country:Japan  

  • 希少糖 5-ケト-D-フルクトースの油脂酵母 Lipomyces starkeyi における代謝経路の解析

    野寄裕暉慧、佐藤里佳子、高久洋暁、竹下 圭、竹川 薫

    日本農芸化学会2022年度西日本支部大会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学文教キャンパス   Country:Japan  

  • 黄麹菌生細胞におけるグルコアミラーゼ mRNA の時空間的局在解析

    守田湧貴、竹川 薫、樋口裕次郎

    日本農芸化学会2022年度西日本支部大会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学文教キャンパス   Country:Japan  

  • 黄麹菌 Aspergillus oryzae のエンドサイトーシス関連 AAA ATPase AipA の相互作用タンパク質の探索

    日浅怜子、竹川 薫、樋口裕次郎

    日本農芸化学会2022年度西日本支部大会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学文教キャンパス   Country:Japan  

  • 酵母 Yarrowia lipolytica における細胞壁および分泌糖タンパク質の N-型糖鎖構造の解析

    吉松朋紀、田中 大、大橋貴生、福永嵩大、福田良一、竹川 薫

    日本農芸化学会2022年度西日本支部大会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学文教キャンパス   Country:Japan  

  • ガラクトース資化能を有する分裂酵母変異株の遺伝子発現解析

    新原麻結、角井康貢、佐藤政充、竹川 薫

    第55回酵母遺伝学フォーラム研究報告会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:沖縄科学技術大学院大学   Country:Japan  

  • メタノール資化酵母 Ogataea polymorpha の M 期制御機構の解析

    前川裕美、福山 和、Lisa-Marie、Shen Jiangyan、竹川 薫

    第55回酵母遺伝学フォーラム研究報告会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄科学技術大学院大学   Country:Japan  

  • Schizosaccharomyces japonicus 細胞表層におけるガラクトース糖鎖の機能解析

    福永嵩大、大橋貴生、田中 大、吉松朋紀、竹川 薫

    第55回酵母遺伝学フォーラム研究報告会  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:沖縄科学技術大学院大学   Country:Japan  

  • 黄麹菌におけるアミラーゼmRNAの生細胞可視化解析

    守田湧貴、竹川 薫、樋口裕次郎

    第44回蛋白質と酵素の構造と機能に関する九州シンポジウム  2022.8 

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    Event date: 2022.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎県矢太楼南館   Country:Japan  

  • 黄麹菌のエンドサイトーシス経路における初期エンドソーム動態の生理機能解析

    幡野若奈, 竹川 薫, 樋口裕次郎

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Barnesiella属腸内細菌ゲノムに存在する エンドグリコシダーゼの諸性質に関する研究

    土井佳奈子、篠田あかり、中山二郎、竹川 薫

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 酢酸菌が生産する希少糖5-ケトフルクトースの酵母による代謝経路の解析

    野寄裕暉慧、竹下 圭、竹川 薫

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母GPI-アンカー型アミラーゼホモログの機能に関する研究

    吉川莉乃、中北慎一、竹川 薫

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麴菌におけるエルゴチオネイン生合成酵素の細胞内局在解析

    井上慶士, 杉本憲亮, 竹川 薫

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌におけるアクチンケーブルに関する細胞内局在解析

    金橋毬乃, 守田湧貴, 竹川 薫, 樋口裕次郎

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌における初期エンドソーム動態とオートファジーに関する解析,

    黒瀬 葵, 幡野若奈, 平川優希, 竹川 薫, 樋口裕次郎

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌における光遺伝学的手法による細胞内タンパク質局在制御の適用,

    河西建輔, 竹川 薫, 樋口裕次郎

    第59回化学関連支部合同九州大会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 細胞膜タンパク質の同時多色検出を可能にする「ヒト細胞直交性」加水分解酵素の開拓

    山中皓太、金子諒右、立石宙也、谷戸謙太、新居輝樹、岸村顕広、神谷典穂、竹川薫、森健、片山佳樹

    日本分析化学会  2022.5 

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    Event date: 2022.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:茨城大学水戸キャンパス   Country:Japan  

  • GH159酵素のβガラクトフラノシダーゼ活性の機能解析

    小泉 舞華、Shen Jiangyan、田中 大、松永 恵美子、伊藤 文恵、佐々木 雅人、竹川 薫、柴田 信之

    日本薬学会第142年会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Structures and functions of cell wall polysaccharides in various yeast species Invited

    Kaoru Takegawa

    Internationa Symposium on Microbial Glycoconjugates and the GySpace Alliance: from micro to macro glycoscience.  2022.3 

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    Event date: 2022.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 黄麹菌におけるα-アミラーゼmRNAのライブセルイメージング

    守田 湧貴,竹川 薫,樋口 裕次郎

    日本農芸化学会2022年度大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母における複数の液胞タンパク質輸送に関わる新規VPS遺伝子の解析

    稲川智章、大久保和真、樋口裕次郎、竹川 薫

    日本農芸化学会2022年度大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 糖鎖をめぐる微生物の生存戦略 Invited

    竹川 薫

    香川大学応用生命化学研究センター第13回公開シンポジウム  2022.2 

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    Event date: 2022.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  • 酢酸菌が生産する希少糖5-KFを資化できる酵母の代謝経路の解析

    野寄裕暉慧、竹下 圭、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzae のエンドサイトーシス関連因子AipA とAoAbp1がエンドサイトーシスに及ぼす影響の解析

    日浅怜子、竹川 薫、樋口裕次郎

    第20回糸状菌分子生物学コンファレンス  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzae におけるegfpプローブを用いたsmFISHによるmRNA局在解析

    守田湧貴、竹川 薫、樋口裕次郎

    第20回糸状菌分子生物学コンファレンス  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母ガラクトース資化株の諸性質の解析

    中山伊都、前川裕美、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母のガラクトース資化関連遺伝子の発現解析

    新原麻結、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Yarrowia lipolyticaの糖鎖合成欠損変異株の取得と諸性質の解析

    吉松朋紀、田中 大、大橋貴生、福永嵩大、福田良一、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母ゲノム中のトランスポゾン様領域への外来遺伝子導入

    満田友希子、藤木真優、前川裕美、樋口裕次郎、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母におけるDUF3844ドメイン含有タンパク質の機能解析

    稲川智章、大久保和真、樋口裕次郎、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母におけるガラクトース含有糖鎖の生合成機構解析

    福永嵩大、田中直孝、古本敏夫、中北愼一、大橋貴生、樋口裕次郎、前川裕美、竹川 薫

    第38回YEAST WORKSHOP  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母のGPI-アンカー型アミラーゼホモログ遺伝子破壊株の諸性質の解析

    吉川莉乃、中北慎一、竹川 薫

    第38回YEAST WORKSHOP  2021.10 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • アルキル-L-ソルボシドの皮膚状態悪化要因菌選択的増殖抑制活性について

    竹下 圭、竹川 薫、石井知彦、古本敏夫

    日本防菌防黴学会第48回年次大会  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるsmFISH法を用いたEGFP融合タンパク質とmRNAの局在解析

    守田 湧貴,竹川 薫,樋口 裕次郎

    日本農芸化学会西日本・中四国・関西支部 2021年度合同鹿児島大会  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeのエンドサイトーシスにおけるAipAとAoAbp1の関係性の解析

    日浅 怜子、竹川 薫、樋口 裕次郎

    第12回トランスポーター研究会九州部会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける光遺伝学的手法導入の試み

    河西 建輔,守田 湧貴,竹川 薫,樋口 裕次郎

    第58回化学関連支部合同九州大会  2021.7 

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    Event date: 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Characterization and substrate soecificity of family 159 glycoside hydrolases from bacte-rial genome databases.

    Shen J, Matsunaga E, Takegawa K

    2021.7 

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    Event date: 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 酢酸菌が生産する希少糖5-KFの分裂酵母による資化経路の解析

    野寄裕暉慧、山下諒子、竹下 圭、竹川 薫

    第58回化学関連支部合同九州大会  2021.7 

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    Event date: 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける後期エンドソームに関わるRab GTPaseの解析

    杉本 憲亮,竹川 薫,樋口 裕次郎

    第58回化学関連支部合同九州大会  2021.7 

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    Event date: 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態と液胞形成に関する解析

    幡野 若奈,高田 歩未,竹川 薫,樋口 裕次郎

    第58回化学関連支部合同九州大会  2021.7 

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    Event date: 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母における推定αガラクトース転移酵素10遺伝子の基質特異性解析

    #福永嵩大、田中直孝、古本敏夫、中北愼一、大橋貴生、樋口裕次郎、前川裕美、竹川 薫

    令和3年度日本生化学会九州支部例会  2021.6 

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    Event date: 2021.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 分裂酵母に特有な新規VPS遺伝子の機能解析

    #稲川 智章,大久保 和真,樋口 裕次郎,竹川 薫

    日本農芸化学会2021年度仙台大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学(一部オンライン)   Country:Japan  

  • Schizosaccharomyces pombeにおける推定α-ガラクトース転移酵素10遺伝子の機能解析

    #福永嵩大、田中直孝、古本敏夫、中北愼一、大橋貴生、樋口裕次郎、前川裕美、竹川 薫

    日本農芸化学会2021年度仙台大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学(一部オンライン)   Country:Japan  

  • 黄麹菌Aspergillus oryzaeのエンドサイトーシスにおけるAAA ATPaseAipAの分子機構解析

    #日浅 怜子,竹川 薫,樋口 裕次郎

    日本農芸化学会2021年度仙台大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学(一部オンライン)   Country:Japan  

  • 黄麹菌 Aspergillus oryzae におけるAoCdc48の生理機能解析

    #守田 湧貴,竹川 薫,樋口 裕次郎

    日本農芸化学会2021年度仙台大会  2021.3 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学(一部オンライン)   Country:Japan  

  • Analysis of unconventional protein secretion for AoSod1 in Aspergillus oryzae.

    Kubota K, Takegawa K, Higuchi Y

    JSBBA West 3rd Student Forum  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Association analysis between early endosome dynamics and kojic acid secretory produc-tion in Aspergillus oryzae.

    #Hirakawa Y, Takegawa K, Higuchi Y

    JSBBA West 3rd Student Forum  2020.12 

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    Event date: 2020.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Immobilization of 1,6-α-L-fucosidase and endo-beta-N-acetylglucosaminidase for defuco-sylation of N-glycan.

    2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • Integration of foreign genes into transposon-like gene sequences in fission yeast.

    JSBBA West 3rd Student Forum  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of outcrossing and inbreeding in Ogataea polymorpha.

    #Kai N, Takegawa K, Maekawa H

    JSBBA West 3rd Student Forum  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 酸性糖鎖の構成成分としてのピルビン酸の生物界における分布と機能

    竹川 薫

    比較グライコーム研究会  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:帯広畜産大学(一部オンライン)   Country:Japan  

  • Ogataea polymorpha における SPOC 機構の解析

    #福山 和,竹川 薫,前川裕美

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるエンドサイトーシス関連AAA ATPaseAipAとAoAbp1の機能解析

    #日浅怜子,竹川 薫,樋口裕次郎

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 黄麹菌のエンドサイトーシス経路における初期エンドソーム動態の寄与

    #高田歩未,竹川 薫,樋口裕次郎

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • フザリウム属糸状菌の細胞壁および糖タンパク質に存在するグルクロン酸含有糖鎖を分解する酵素の探索

    #古賀朋美,樋口裕次郎,竹川 薫

    2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態とコウジ酸生産性に関する解析

    #平川優希,竹川 薫,樋口裕次郎

    糸状菌分子生物学研究会若手の会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAoSod1タンパク質特殊分泌に関する解析

    #久保田夏帆,竹川 薫,樋口裕次郎

    糸状菌分子生物学研究会若手の会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeのタンパク質品質管理に関与するAoCdc48の機能性解析

    #守田湧貴, 竹川 薫, 樋口裕次郎

    糸状菌分子生物学研究会若手の会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeのエンドサイトーシス関連因子AipAとAoAbp1の機能解析

    #日浅怜子、柿本健一、竹川 薫、樋口裕次郎

    糸状菌分子生物学研究会若手の会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄麹菌Aspergillus oryzaeの初期エンドソーム動態に関する細胞内生理機能解析

    #高田歩未, 竹川 薫, 樋口裕次郎

    糸状菌分子生物学研究会若手の会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • Schizosaccahromyces属4種の細胞表層糖鎖の構造比較解析

    #福永嵩大、田中直孝、古本敏夫、中北愼一、大橋貴生、樋口裕次郎、前川裕美、竹川 薫

    令和2年度日本生化学会九州支部例会  2020.5 

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    Event date: 2020.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 中性領域で働く基質特異性の広い万能シアリダーゼの発見と性質

    岩木佑弥、松永恵美子、竹川 薫、佐藤ちひろ、北島 健

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • Schizosaccharomyces属における細胞表層糖鎖の構造解析

    福永嵩大、田中直孝、中北慎一、樋口裕次郎、前川裕美、竹川 薫

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • Aspergillus nidulansが生産する2つのβ-D-ガラクトフラノシダーゼの基質特異性および諸性質の解析

    松永恵美子、豊田早紀、山田久恵、田中 大、樋口裕次郎、竹川 薫

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • N結合型糖鎖欠損糖タンパク質を分泌生産する麹菌AoGlycoDelete株の諸性質解析

    李 秋実、田辺佳奈、小谷哲也、竹川 薫、樋口裕次郎

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAAAATPaseAipAとAoAbp1のエンドサイトーシスにおける機能解析

    日浅怜子、柿本健一、竹川 薫、樋口裕次郎

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAoCdc48の機能及び有用物質分泌生産能に関する解析

    守田湧勇、菊松風大、竹川 薫、樋口裕次郎

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • 黄麹菌におけるα-アミラーゼmRNAの可視化解析

    樋口裕次郎、竹川 薫

    日本農芸化学会2020年度福岡大会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • サナギタケ由来糖鎖切断酵素の結晶構造解析 Invited

    関 陽香、黄 一博、荒川孝俊、山田千早、木下崇司、岩本将吾、樋口裕次郎、竹川 薫、伏信進矢

    第11回MLFシンポジウム第37回PFシンポジウム  2020.3 

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    Event date: 2020.3

    Language:Japanese  

    Venue:ザ・ヒロサワ・シティ会館(茨城県県立市民文化センター)   Country:Japan  

  • Substrate specificity and structure of microbial endo-β-N-acetylglucosaminidases. Invited International conference

    2020.3 

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    Event date: 2020.3

    Language:English  

    Venue:Soka University   Country:Japan  

  • Fusarium属糸状菌の生産するグルクロン酸含有分泌多糖を分解する酵素の探索

    古賀朋美、樋口裕次郎、竹川 薫

    日本生物工学会第26回九州支部長崎大会  2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学水産学部   Country:Japan  

  • 分裂酵母の核細胞質で機能するマンノース転移酵素Omh6pの機能解析

    下村琴音、前川裕美、竹川 薫

    日本生物工学会第26回九州支部長崎大会  2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学水産学部   Country:Japan  

  • 分裂酵母特有なアルカリストレス応答および遺伝子発現機構の解析

    森 日香里、冨永陽大、樋口裕次郎、竹川 薫

    日本生物工学会第26回九州支部長崎大会  2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学水産学部   Country:Japan  

  • 黄麹菌Aspergilus oryzaeにおけるAAA ATPase AipAはエンドサイトーシスの制御に関わっている

    日浅怜子、柿本健一、竹川 薫、樋口裕次郎

    日本生物工学会第26回九州支部長崎大会  2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学水産学部   Country:Japan  

  • Identification of a novel gene required for vacuolar protein transport in fission yeast.

    Inagawa T, Okubo K, Higuchi Y, Takegawa K

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of the mating-type switiching mechanism in Ogataea polymorpha.

    Kai N, Takegawa K, Maekawa H

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis on the relationship between early endosome dynamics and other organelles in Aspergillus oryzae.

    Takata A, Takegawa K, Higuchi Y

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Functional analysis of putative glycosyl transferase Omh6p localized to the nucleus and cytoplasm in fission yeast.

    Shimomura K, Maekawa H, Takegawa K

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Production of human DNaseI using the fission yeast expression system.

    Iwai R, Murai K, Maekawa H, Higuchi Y, Takegawa K

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of spindle orientation checkpoint mechanism in methylotrophic yeast Ogataea polymorpha.

    Fukuyama N, Takegawa K, Maekawa H

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of substrate specificity of putative α-L-arabinofuranosidases in Aspergillus nidu-lans.

    2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:Fukuoka City Science Museum (福岡市科学館)   Country:Japan  

  • Involvement of AoCdc48 in useful material productivity in Aspergillus oryzae.

    Morita Y, Kikumatsu F, Takegawa K, Higuchi Y

    JSBBA West 2nd Student Forum  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 酢酸菌の生産する希少糖5-ケトフルクトースを介した酵母との相互作用解析

    山下諒子、竹下 圭、竹川 薫

    日本農芸化学会2019年度西日本・中四国支部合同沖繩大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • Asperdillus nidulansゲノム中に存在するα-L-アラビノフラノシダーゼ関連酵素の基質特異性の解析

    山田久恵,松永恵美子,樋口裕次郎,竹川 薫

    日本農芸化学会2019年度西日本・中四国支部合同沖繩大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態と他の細胞小器官との関連性解析

    高田歩未、竹川 薫、樋口裕次郎

    第19回糸状菌分子生物学コンファレンス  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeの分泌経路におけるSMタンパク質の機能解析

    原 爽太郎、竹川 薫、樋口裕次郎

    第19回糸状菌分子生物学コンファレンス  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるN結合型欠損糖タンパク質分泌生産株の解析

    李 秋実、竹川 薫、樋口裕次郎

    第19回糸状菌分子生物学コンファレンス  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAoCdc48の機能解析

    守田湧貴、菊松風大、竹川 薫、樋口裕次郎

    第19回糸状菌分子生物学コンファレンス  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道大学   Country:Japan  

  • 酢酸菌における希少糖5−ケトフルクトース(5-KF)の生合成経路の解明および5-KFを介した酵母との相互作用解析

    山下諒子、竹下 圭、竹川 薫

    第37回イーストワークショップ  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:熊本大学   Country:Japan  

  • 分裂酵母ゲノムに存在するトランスポゾン領域への外来遺伝子組込み効率向上の検討

    満田友希子、藤木真優、前川裕美、樋口裕次郎、竹川 薫

    第37回イーストワークショップ  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:熊本大学   Country:Japan  

  • 分裂酵母特有の液胞タンパク質輸送関連遺伝子SPBC1709.03の機能解析

    稲川智章、大久保和真、樋口裕次郎、竹川 薫

    第37回イーストワークショップ  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:熊本大学   Country:Japan  

  • 分裂酵母の核と細胞質に局在するマンノース転移酵素Omh6pの機能解析

    下村琴音、前川裕美、竹川 薫

    第37回イーストワークショップ  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:熊本大学   Country:Japan  

  • 分裂酵母に特有なアルカリストレス応答経路の解明

    森 日香里、富永陽大、樋口裕次郎、竹川 薫

    第37回イーストワークショップ  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:熊本大学   Country:Japan  

  • 黄麹菌におけるN結合型糖鎖欠損糖タンパク質分泌株の解析

    李 秋実、竹川 薫、樋口 裕次郎

    第43回蛋白質と酵素の構造と機能に関する九州シンポジウム  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡市国民宿舎マリンテラスあしや   Country:Japan  

  • Schizosaccharomyces pombeにおけるピルビン酸含有糖鎖生合成経路の解析

    福永嵩大、樋口裕次郎、前川裕美、田中直孝、竹川 薫

    第52回酵母遺伝学フォーラム研究報告会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:静岡県清水文化会館マリナート   Country:Japan  

  • 分裂酵母に特有なアルカリストレス応答経路の解析

    森 日香里、樋口裕次郎、竹川 薫

    第52回酵母遺伝学フォーラム研究報告会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:静岡県清水文化会館マリナート   Country:Japan  

  • Characterization of a novel galactosidase acting on arabinogalactan of Mycobacterium tuberculosis. International conference

    Shen L, Viljoen A, Villaume S, Chene L, Vincent S, Takegawa K, Joe M, Lowary TL, Kre-mer L, Guerardel Y, Mariller C

    25th International Symposium On Glycoconjugates  2019.8 

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    Event date: 2019.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Italy  

  • Endo-SB, Endo-CoM変異型における糖転移活性の基質特異性について

    三谷 藍、住吉 渉、木下崇司、竹川 薫

    第38回日本糖質学会年会  2019.8 

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    Event date: 2019.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋大学   Country:Japan  

  • 基質特異性の広いSphingobacterium属由来万能シアリダーゼの発見と性質解析

    岩城佑弥、竹川 薫、佐藤ちひろ、北島 健

    第38回日本糖質学会年会  2019.8 

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    Event date: 2019.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋大学   Country:Japan  

  • Aspergillus属糸状菌の菌糸体形成に重要なβ-D-ガラクトフラノシダーゼの役割

    松永恵美子、山田久恵、樋口裕次郎、岡 拓二、田中 大、竹川 薫

    第38回日本糖質学会年会  2019.8 

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    Event date: 2019.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋大学   Country:Japan  

  • Production of heterologous glycoproteins in fission yeast and transglycosylation of com-plex-type oligosaccharides using microbial endoglycosidases. Invited International conference

    Takegawa K, Huang Y, Kinoshita T, Mitani A, Eshima Y, Higuchi Y

    Joint International Symposium of Korean Scociety of Life Science and Interdisciplinary Society of Genetic & Genomic Medicine  2019.8 

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    Event date: 2019.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Korea, Republic of  

  • The class C Vps complex functions at multiple stages of the vacuolar transport pathway in Schizosaccharomyces pombe. Invited International conference

    Takegawa K

    EMBO Workshop on Fission Yeast 10th International Meeting (POMBE2019)  2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Spain  

  • 糸状菌Aspergillus nidulansの菌糸体形成におけるβ-D-ガラクトフラノシダーゼの役割について

    山田久恵, 豊田早紀, 松永恵美子, 樋口裕次郎, 竹川 薫

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAocdc48変異株の表現型解析

    守田湧貴, 菊松風大, 竹川 薫, 樋口裕次郎

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるタンパク質特殊分泌経路関連因子の解析

    久保田夏帆, Kwon HeeSu, 竹川 薫, 樋口裕次郎

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeの固体培養における初期エンドソーム動態の寄与

    高田歩未, 竹川 薫, 樋口裕次郎

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母の細胞質と核に局在するマンノース転移酵素Omh6pの機能解析

    下村琴音, 前川裕美, 竹川 薫

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるコウジ酸生産と初期エンドソーム動態との関連性解析

    平川優希, 竹川 薫, 樋口裕次郎

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • メタノール資化酵母Ogatae polymorphaに特徴的な染色体逆位を介した接合型変換機構の解析

    甲斐菜摘, 福山 和, 前川裕美, 竹川 薫

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母のゲノムに存在するトランスポゾン様遺伝子配列への外来遺伝子の組込み

    満田友希子, 藤木真優, 前川裕美, 樋口裕次郎, 竹川 薫

    第56回化学関連支部合同九州大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 基質特異性の異なる微生物由来ENGaseの比較

    竹川 薫

    第20回比較グライコーム研究会  2019.6 

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜市帆船日本丸メモリアルパーク   Country:Japan  

  • Schizosaccharomyces 属におけるピルビン酸含有糖鎖の構造解析

    福永嵩大、樋口裕次郎、前川裕美、田中直孝、竹川 薫

    日本農芸化学会 2019年度大会  2019.3 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農業大学   Country:Japan  

  • 非性的凝集素Gsf2を介した分裂酵母の細胞間認識機構の解析

    藤野友輔,樋口裕次郎,竹川 薫

    第25回日本生物工学会九州支部鹿児島大会  2018.12 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母に特有なアルカリストレス応答と遺伝子発現機構の解析

    森日香里,富永陽大,樋口裕次郎,竹川 薫

    第25回日本生物工学会九州支部鹿児島大会  2018.12 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • 昆虫病原糸状菌Beauveria bassianaゲノムに存在するGH18ファミリーエンドグリコシダーゼの諸性質の解析

    野田滉陽,黄 一博,樋口裕次郎,竹川 薫

    第25回日本生物工学会九州支部鹿児島大会  2018.12 

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    Event date: 2018.12 - 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • Analysis of molecular mechanism of functionally unknown AAA ATPase in eukaryotic mi-croorganisms.

    Hiasa R, Kakimoto K, Takegawa K, Higuchi Y

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Microbial a-L-rhamnosidase from GH78 and GH106 families revealed dual a-L-rhamnosidase and a-L-mannosidase hydrolyzing activity.

    Papali-Tautau A, Matsunaga E, Izumi M, Higuchi Y, Takegawa K

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Functional analysis of Bub2 checkpoint in the methylotropic yeast Ogataea polymorpha.

    Bienes K, Takegawa K, Maekawa H

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of alkaline stress response and gene expression mechanism in Schizosaccha-romyces pombe.

    Mori H, Tominaga A, Higuchi Y, Takegawa K

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of proteins involved in the biosynthesis of pyruvylated oligosaccharides in fis-sion yeasts.

    Fukunaga T, Higuchi Y, Maekawa H, Takegawa K

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of production mechanism of 5-keto-D-fructose by acetic acid bacteria.

    Yamashita R, Higuchi Y, Maekawa H, Takegawa K

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Analysis of the mating-type switching mechanism in Ogataea polymorpha.

    Fukuyama N, Takegawa K, Maekawa H

    JSBBA West 1st Student Forum  2018.11 

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    Event date: 2018.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 黄麹菌 Aspergillus oryzaeにおける有用物質生産に関与する SM タンパク質の解析

    原爽太郎、竹川 薫、樋口裕次郎

    第18回糸状菌分子生物学コンファレンス  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シティホールプラザアオーレ長岡   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における Cdc48 オルソログの有用物質生産性への関与

    菊松風大、竹川 薫、樋口裕次郎

    第18回糸状菌分子生物学コンファレンス  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:シティホールプラザアオーレ長岡   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における糖鎖欠損型分泌糖タンパク質生産

    李 秋実、竹川 薫、樋口裕次郎

    第18回糸状菌分子生物学コンファレンス  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シティホールプラザアオーレ長岡   Country:Japan  

  • 分裂酵母による有用物質生産―できることとできないことー Invited

    竹川 薫

    第16回新産業酵母研究会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:産総研・臨海副都心センター   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombe によるヒトDNaseⅠ生産条件の検討

    村井香織、福谷早紀、前川裕美、樋口裕次郎、竹川 薫

    第36回イーストワークショップ  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:愛媛大学大学会館   Country:Japan  

  • Schizosaccharomyces属における細胞表層糖鎖のピルビン酸付加に関与するタンパク質の解析

    福永嵩大、樋口裕次郎、前川裕美、竹川 薫

    第36回イーストワークショップ  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:愛媛大学大学会館   Country:Japan  

  • 分裂酵母の細胞質局在推定マンノース転移酵素をコードするomh6遺伝子破壊株の諸性質の解析

    下村琴音、前川裕美、竹川 薫

    第36回イーストワークショップ  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:愛媛大学大学会館   Country:Japan  

  • メタノール資化性酵母Ogataea polymorphaの接合型変換機構の解析

    福山 和、竹川 薫、前川裕美

    第36回イーストワークショップ  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:愛媛大学大学会館   Country:Japan  

  • 微量香気成分インドールが本格焼酎の官能特性に及ぼす影響

    梶原康博、大石雅志、竹川 薫、高下秀春

    平成30年度日本醸造学会大会  2018.10 

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    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学弥生講堂   Country:Japan  

  • 糖タンパク質の糖鎖を根本から切断する生物学的意味を考える

    竹川 薫

    第4回ENGase研究会  2018.10 

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    Event date: 2018.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:理化学研究所(和光市)   Country:Japan  

  • 麹菌Aspergillus oryzaeにおけるCdc48オルソログと物質生産性の解析

    菊松風大、竹川 薫、樋口裕次郎

    農芸化学会平成30年度西日本支部大会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母細胞表層糖鎖のピルビン酸付加に関与する推定β1,3-ガラクトース転移酵素の機能解析

    福永嵩大、樋口裕次郎、前川裕美、竹川 薫

    農芸化学会平成30年度西日本支部大会  2018.9 

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    Event date: 2018.9

    Language:Japanese  

    Venue:崇城大学   Country:Japan  

  • 子嚢菌Cordyceps militaris 由来GH18エンド-β-N-アセチルグルコサミニダーゼの結晶構造解析

    関 陽香、荒川考俊、黄一博、樋口裕次郎、江島康成、竹川 薫、伏信進也

    日本応用糖質科学会平成30年度大会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:秋田県立大学生物資源科学部秋田キャンパス   Country:Japan  

  • 分裂酵母のアルカリストレス応答と遺伝子発現機構の解析

    森 日香里、富永陽大、樋口裕次郎、竹川 薫

    酵母遺伝学フォーラム第51回研究報告会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • Aspergillus nidulansに存在する2つのβ-D-ガラクトフラノシダーゼの機能解析

    松永恵美子、豊田早紀、山田久恵、田中 大、樋口裕次郎、竹川 薫

    第37回日本糖質学会年会  2018.8 

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    Event date: 2018.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:仙台国際センター   Country:Japan  

  • Aspergillus nidulansに存在するβ-D-ガラクトフラノシダーゼの解析

    松永恵美子、豊田早紀、岡 拓二、樋口裕次郎、竹川 薫

    日本生化学会平成30年度九州支部例会  2018.7 

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    Event date: 2018.6 - 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態欠損株の諸性質解析

    髙田歩未、都甲祐介、竹川薫、樋口裕次郎

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母のアルカリストレス応答と遺伝子発現機構の解析

    森 日香里、富永陽大、樋口裕次郎、竹川 薫

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Aspergillus nidulansにおけるβ-D-ガラクトフラノシダーゼ遺伝子発現調節機構の解析

    山田久恵、豊田早紀、松永恵美子、樋口裕次郎、竹川 薫

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Functional characterization of pyruvyltransferase Pvg1 in fission yeast species

    Risa Shofia, Fukunaga, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa

    2018.6 

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    Event date: 2018.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • メタノール資化酵母Ogataea polymorphaがもつユニークな接合型システムの解析

    福山 和、竹川 薫、前川裕美

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Evolutional conservation of mitotic regulation in the methylotrophic yeast Ogataea poly-morpha.

    Kristina Bienes, Kaoru Takegawa, Hiromi Maekawa

    2018.6 

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    Event date: 2018.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 黄麹菌Aspergillus oryzaeによる異種タンパク質生産における発現コンストラクトの影響

    白澤慧祐, 李秋実, 竹川薫, 樋口裕次郎

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母の推定マンノース転移酵素をコードするomh6遺伝子破壊株の諸性質の解析

    下村琴音、前川裕美、竹川 薫

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態のコウジ酸分泌への関与

    都甲祐介、竹川 薫、樋口裕次郎

    日本農芸化学会2018年度大会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学   Country:Japan  

  • 糖類や糖鎖を介した微生物と高等動物の相互作用

    竹川 薫

    日本農芸化学会2018年度大会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名城大学   Country:Japan  

  • 天然型単糖メチル-L-ソルボシドの機能性について

    竹下 圭、古本敏夫、竹川 薫

    かがわ糖質バイオフォーラム第10回シンポジウム  2018.1 

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    Event date: 2018.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:かがわ国際会議場   Country:Japan  

  • 微生物に特有な細胞表層糖鎖の合成と分解に関わる酵素の利用 Invited

    竹川 薫

    かがわ糖質バイオフォーラム、複合糖質・糖鎖研究会  2018.1 

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    Event date: 2018.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川県高松市   Country:Japan  

  • 糖タンパク質の糖鎖構造を均一にする技術 Invited

    竹川 薫

    九州大学農学部・大学院生物資源環境科学府・大学院農学研究院 「新キャンパス」キックオフ  2018.12 

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    Event date: 2017.12 - 2018.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本橋ライフサイエンスビル   Country:Japan  

  • 糸状菌Aspergillus nidulansの糖代謝に関わる-D-Galactofuranosidaseの機能解析

    豊田早紀、八色奈央、松永恵美子、樋口裕次郎、岡 拓ニ、後藤正利、竹川 薫

    第24回生物工学会九州支部沖縄大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • 白麹菌におけるZn(II)2Cys6型推定転写因子NosAとRosAの機能解析

    木本大地、門岡千尋、田代智史、梶原康博、髙下秀春、竹川薫

    第24回生物工学会九州支部沖縄大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • 抗体のN-結合型糖鎖を効率良く遊離可能な新しい特異性を持ったエンドグリコシダーゼの特性解析

    Yibo Huang、樋口裕次郎、木下崇司、三谷 藍、江島康成、竹川 薫

    第24回生物工学会九州支部沖縄大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • Aspergillus nidulansに存在する-D-ガラクトフラノシダーぜ活性をもつ酵素の探索

    松永恵美子、小野健太郎、豊田早紀、樋口裕次郎、竹川 薫

    第24回生物工学会九州支部沖縄大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:琉球大学   Country:Japan  

  • Schizosaccharomyces pombeにおけるAAA ATPaseの薬剤排出に関する機能解析

    日浅怜子、宋 原準、樋口裕次郎、竹川 薫

    第35回YEAST WORKSHOP  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川県高松市   Country:Japan  

  • 分裂酵母における鉄および銅イオンの取り込みとpHストレス条件での生育に関する解析

    森 日香里、久保田健夫、樋口裕次郎、竹川 薫

    第35回YEAST WORKSHOP  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川県高松市   Country:Japan  

  • Ogataea polymorphaの接合型変換とその制御機構の解析

    前川裕美、山本勝良、Thi TN Tran、金子嘉信、竹川 薫

    第35回YEAST WORKSHOP  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川県高松市   Country:Japan  

  • 新しい基質特異性を持ったエンドグリコシダーゼの探索とその応用 Invited

    竹川 薫

    第8回グライコバイオロジクス研究会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢商工会議所   Country:Japan  

  • Aspergillus nidulans の糖代謝に関連する β-D-Galactofuranosidase の機能解析

    豊田早紀,八色奈央,松永恵美子,樋口裕次郎,岡 拓二,後藤正利,竹川 薫

    第17回糸状菌分子生物学コンファレンス  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:佐賀市立東与賀文化ホール   Country:Japan  

  • Aspergillus oryzae における分泌経路に関与する SM タンパク質の解析

    原爽太朗,竹川 薫,樋口裕次郎

    第17回糸状菌分子生物学コンファレンス  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:佐賀市立東与賀文化ホール   Country:Japan  

  • 黄麹菌 Aspergillus oryzae のエンドサイトーシス関連タンパク質 AipA 及び相互作用因子の解析

    柿本健一,竹川 薫, 樋口裕次郎

    第17回糸状菌分子生物学コンファレンス  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:佐賀市立東与賀文化ホール   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における小胞体関連分解因子に関する解析

    菊松風大,竹川 薫,樋口裕次郎

    第17回糸状菌分子生物学コンファレンス  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:佐賀市立東与賀文化ホール   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における初期エンドソーム動態の有用物質生産への関与

    都甲祐介,竹川薫,樋口裕次郎

    第17回糸状菌分子生物学コンファレンス  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:佐賀市立東与賀文化ホール   Country:Japan  

  • Analysis of unconventional protein secretion of acyl-CoA binding protein in Aspergillus oryzae

    Hee Su K, Kawaguchi K, Kikuma T, Kitamoto K, Takegawa K, Higuchi Y

    2017.11 

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    Event date: 2017.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Endo-SBs, novel endo-β-N- acetylglucosaminidases from Sphingobacterium species with fucose-containing oligosaccharides-specific endoglycosidase activity

    2017.11 

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    Event date: 2017.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • Functional analysis of two galactofuranosidases in Aspergillus nidulans

    Toyota S, Yairo N, Matsunaga E, Higuchi Y, Oka T, Goto M, Takegawa K

    AFELiSA 2017  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • α-L- Rhamnosidases from GH78 and GH106 families revealed dual α-L- rhamnose and α-L- mannoside hydrolyzing activity

    2017.11 

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    Event date: 2017.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • ピルビン酸化糖鎖の微生物における生合成と役割 Invited

    竹川 薫

    第15回糖鎖科学コンソーシアムシンポジウム  2017.10 

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    Event date: 2017.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母において鉄および銅イオンの取り込みはpHストレス条件下における生育に重要である

    森日香里,久保田健夫,樋口裕次郎,竹川薫

    日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学   Country:Japan  

  • Aspergillus nidulansに存在するα-L-アラビノフラノシダーゼの探索

    松永恵美子,小野健太郎,豊田早紀,樋口裕次郎,竹川薫

    日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学   Country:Japan  

  • Identification and characterization of novel fucose-containing oligosaccharides-specific endo-β-N-acetylglucosaminidases from Sphingobacterium species

    日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会  2017.9 

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    Event date: 2017.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学   Country:Japan  

  • Microbial α-L-rhamnosidases reveal dual α-L-rhamnose and α-L-mannose hydrolyz-ing activity

    日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会  2017.9 

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    Event date: 2017.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学   Country:Japan  

  • ⻩麹菌Aspergillus oryzaeの固体培養における初期エンドソーム動態に関する解析

    都甲祐介,樋口裕次郎,竹川薫

    日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪府立大学   Country:Japan  

  • Molecular dissection of unconventional secretion of acyl-CoA binding protein in Aspergillus oryzae

    Hee Su K, Kawaguchi K, Kikuma T, Kitamoto K, Takegawa K, Higuchi Y

    2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Aspergillus oryzae が有する 2 つのアシル CoA 結合タンパク質の細胞生化学的解析

    樋口裕次郎、クウォン ヒースー、川口航平、菊間隆志、北本勝ひこ、竹川 薫

    第69回日本生物工学会大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:早稲田大学   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における分泌糖タンパク質に関する解析 Invited

    李 秋実、竹川 薫、樋口裕次郎

    第69回日本生物工学会大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:早稲田大学   Country:Japan  

  • 分裂酵母を宿主とした異種糖タンパク質の分泌生産システムの構築

    福谷早紀、陶山明子、中北愼一、片倉喜範、樋口裕次郎、竹川 薫

    酵母遺伝学フォーラム第50回研究報告会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • メタノール資化酵母Ogataea polymorphaの接合型変換に関与する因子の検索

    山本勝良、Thi N. M. Tran、竹川薫、金子嘉信、前川裕美

    酵母遺伝学フォーラム第50回研究報告会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • Aspergillus nidulansに存在するα-L-アラビノフラノシダーゼの諸性質の解析

    松永恵美子、小野健太郎、八色奈央、豊田早紀、樋口裕次郎、竹川 薫

    第36回日本糖質学会年会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:旭川市民文化会館   Country:Japan  

  • 分裂酵母のアルカリー中性条件下における生育におよぼす鉄および銅イオンの影響について

    森 日香里、久保田健夫、樋口裕次郎、竹川 薫

    第54回化学関連支部合同九州大会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Unconventional secretion of acyl-CoA binding protein in Aspergillus oryzae

    Kwon Hee Su, Kouhei Kawaguchi, Takashi Kikuma, Katsuhiko Kitamoto, Kaoru Takega-wa, Yujiro Higuchi

    2017.7 

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    Event date: 2017.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母における非性的凝集素Gsf2のガラクトース鎖認識部位の探索

    藤野友輔、陶山明子、樋口裕次郎、竹川 薫

    第54回化学関連支部合同九州大会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母ゲノム上に存在するガラクトース転移酵素の機能解析

    吉田武史、樋口裕次郎、竹川 薫

    第54回化学関連支部合同九州大会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • グリコシダーゼを微生物から探索するー問題点と解決法—

    竹川 薫

    比較グライコーム研究会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学伊都キャンパス   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおいて初期エンドソーム動態は細胞の分化およびタンパク質分泌に関与する

    樋口 裕次郎, 都甲祐介, 竹川 薫

    2017年度日本農芸化学会大会  2017.3 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるエンドサイトーシス関連因子AipAの機能解析

    柿本健一, 竹川 薫, 樋口 裕次郎

    第16回糸状菌分子生物学コンファレンス  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宇治おうばくプラザ   Country:Japan  

  • Dissection of acyl-CoA binding protein secretion in Aspergillus oryzae

    Hee Su K, Kawaguchi K, Kikuma T, Kitamoto K, Takegawa K, Higuchi Y

    2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Aspergillus fumigatusのガラクトフラノース転移酵素(AfGfsA)の酵素的諸性質の解析

    川満洋平, 李秋実, 田中大, 浴野圭輔, Takegawa K, 後藤正利, 柴田信之, 太田一良, 岡拓二

    第16回糸状菌分子生物学コンファレンス  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宇治おうばくプラザ   Country:Japan  

  • Aspergillus nidulansにおけるβ-D-Galactofuranosidaseの機能解析

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 後藤正利, 竹川 薫

    第16回糸状菌分子生物学コンファレンス  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宇治おうばくプラザ   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態解析

    都甲祐介, 竹川 薫, 樋口 裕次郎

    第16回糸状菌分子生物学コンファレンス  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宇治おうばくプラザ   Country:Japan  

  • 酵素法と化学合成法を用いたガラクトフラノシド誘導体の合成(第ニ報)

    太田涼, 小岩美月, 竹川 薫, 岡 拓ニ, 清田洋正, 泉 実

    2016年日本化学会中国四国支部大会  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川大学   Country:Japan  

  • 分裂酵母の非性的凝集素 Gsf2 のガラクトース鎖認識機構の解析

    藤野友輔, 陶山明子, 樋口 裕次郎, 竹川 薫

    第34回YEAST WORKSHOP  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:島根大学松江キャンパス   Country:Japan  

  • Schizosaccharomyces pombe の液胞タンパク質輸送機構の解析

    大久保和真, 樋口 裕次郎, Takegawa K

    第34回YEAST WORKSHOP  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:島根大学松江キャンパス   Country:Japan  

  • 分裂酵母の液胞膜タンパク質の輸送に重要な Vps18p の機能解析及び 相互作用するタンパク質の探索

    大久保皓平, 落石 悟, 樋口 裕次郎, 竹川 薫

    第34回YEAST WORKSHOP  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:島根大学松江キャンパス   Country:Japan  

  • 分裂酵母 Schizosaccharomyces pombe における代謝改変がクエン酸 生産に及ぼす影響

    桑野梨沙, 陶山明子, 川向 誠, 竹川 薫

    第34回YEAST WORKSHOP  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:島根大学松江キャンパス   Country:Japan  

  • 分裂酵母 Schizosaccharomyces pombe のα1,2-およびα1,3-ガラクトース転移酵素の機能解析

    吉田武史, 樋口 裕次郎, 竹川 薫

    第34回YEAST WORKSHOP  2016.11 

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    Event date: 2016.11 - 2016.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:島根大学松江キャンパス   Country:Japan  

  • Streptomyces属放線菌由来の2つのβ-D-ガラクトフラノシダーぜの比較

    松永恵美子, 八色奈央, 豊田早紀, 岡 拓二, 樋口 裕次郎, Takegawa K

    第68回日本生物工学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:富山国際会議場   Country:Japan  

  • Bacillus 属細菌由来ピルビン酸化ガラクトース含有糖鎖分解酵素の諸性質の解析

    松藤仁美, 樋口 裕次郎, Takegawa K

    第68回日本生物工学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:富山国際会議場   Country:Japan  

  • 糸状菌 Aspergillus nidulans における β-D-Galactofuranosidase の機能解析

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 後藤正利, Takegawa K

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Molecular mechanisms of acyl-CoA binding protein secretion in Aspergillus oryzae

    Hee Su K, Kawaguchi K, Kikuma T, Kitamoto K, Takegawa K, Higuchi Y

    2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 黄麹菌 Aspergillus oryzae におけるエンドサイトーシス関連機能未知タンパク質 AipA の機能解析

    柿本健一, 竹川 薫, 樋口 裕次郎

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Aspergillus nidulans の分生子形成に関与する糖転移酵素様機能未知膜タンパク質の解析

    川満洋平, 浴野圭輔, 竹川 薫, 後藤正利, 太田一良, 岡 拓二

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Aspergillus fumigatus における新規ガラクトフラノース転移酵素の探索および機能解析

    李秋実, 浴野圭輔, 竹川 薫, 後藤正利, 太田一良, 岡 拓二

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Schizosaccharomyces pombe における液胞局在セリンプロテアーゼの輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Microbial α-L-rhamnosidases show a dual L-rhamnose and L-mannose hydrolyzing activity

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Transglycosylation activity of Coprinopsis cinerea endoglycosidase mutants enabling the synthesis of glycoproteins using synthetic sialo-complex-type sugar oxazoline

    Huang Y, Eshima Y, Kinoshita T, Sumiyoshi W, Nakakita S, Higuchi Y, Takegawa K

    2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Bacillus 属細菌由来ピルビン酸化ガラクトース含有糖鎖を分解する酵素の諸性質の解析

    松藤仁美, 伏信進矢, 樋口 裕次郎, Takegawa K

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • 各種微生物由来 β-D-ガラクトフラノシダーゼの諸性質の比較

    松永恵美子, 八色奈央, 豊田早紀, 岡 拓二, 樋口 裕次郎, Takegawa K

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • 分裂酵母の Catechol-O-methyltransferase ホモログタンパク質の諸性質の解析

    赤井真, 中山倫子, 陶山明子, 山田 直隆, 竹川 薫

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2016.9 - 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • Schizosaccharomyces pombe を宿主とした異種糖タンパク質の分泌生産システムの構築

    福谷早紀, 陶山明子, 中北愼一, 樋口 裕次郎, 竹川 薫

    第49回酵母遺伝学フォーラム研究報告会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーサイド舞子ビラ(神戸)   Country:Japan  

  • 分裂酵母における2つのVPS9ドメインタンパク質は低分子量Gタンパク質Rab5のシグナル伝達を協調して制御する

    塚本雄太, 鍵和田 聡, 嶋津小百合, 竹川 薫, 野田哲子, 宮本昌明

    第49回酵母遺伝学フォーラム研究報告会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーサイド舞子ビラ(神戸)   Country:Japan  

  • Schizosaccharomyces pombeにおける液胞局在プロテアーゼの輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    第49回酵母遺伝学フォーラム研究報告会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーサイド舞子ビラ(神戸)   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送に重要なVps18p の機能解析と相互作用するタンパク質の探索

    大久保皓平, 落石 悟, 樋口 裕次郎, 竹川 薫

    第49回酵母遺伝学フォーラム研究報告会  2015.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーサイド舞子ビラ(神戸)   Country:Japan  

  • エンドグリコシダーゼを利用した糖タンパク質糖鎖改変技術 Invited

    竹川 薫

    第35回日本糖質学会年会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:高知市   Country:Japan  

  • Coprinopsis cinerea 由来endo-β-N-acetylglucosaminidase (Endo-CC)の温度、pHの安定性について

    木下崇司, 住吉 渉, 江島康成, 樋口 裕次郎, 中北慎一, 平林 淳, 竹川 薫

    第35回日本糖質学会年会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:高知市   Country:Japan  

  • 糖鎖改変トラスツズマブの調製とその活性

    月村 亘, 森 昌子, 大隅賢二, 戸治野真美, 高嶋 晶, 菅原州一, 弘瀬友理子, 白井 孝, 水野真盛, 天野純子, 黒河内政樹, 工藤 純, 木下崇司, 竹川 薫, 松田昭生

    第35回日本糖質学会年会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:高知市   Country:Japan  

  • Streptomyces属放線菌由来のβ-D-ガラクトフラノシダーぜについて

    松永恵美子, 豊田早紀, 八色奈央, 岡拓二, 樋口 裕次郎, 竹川 薫

    第40回蛋白質と酵素の構造と機能に関する九州シンポジウム  2016.8 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:指宿   Country:Japan  

  • 微生物におけるエンドグリコシダーゼの役割と糖鎖機能解析への応用 Invited

    竹川 薫

    第40回蛋白質と酵素の構造と機能に関する九州シンポジウム  2016.8 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:指宿   Country:Japan  

  • 分裂酵母ホスファチジルイノシトール-4-キナーゼPik1はテロメア保護因子pot1破壊株の生育に必要である

    杉浦あさみ, 治田翔平, 中瀬 舞, 竹川 薫, 上野 勝

    第49回酵母遺伝学フォーラム研究報告会  2016.9 

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    Event date: 2016.8 - 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーサイド舞子ビラ(神戸)   Country:Japan  

  • Vps901 and Vps902, VPS9-containing proteins, cooperate in the regulation of Rab5 GTPase signaling in fission yeast. International conference

    Tsukamoto Y, Kagiwada S, Shimazu S, Takegawa K, Noguchi T, Miyamoto M

    12th International Congress of Cell Biology  2016.7 

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    Event date: 2016.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Czech Republic  

  • 黄麹菌Aspergillus oryzaeにおけるエンドサイトーシス関連AAA ATPase AipAの機能解析

    柿本健一, 竹川 薫, 樋口 裕次郎

    第53回化学関連支部合同九州大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Analysis of secretion machinery of acyl-CoA binding proteins in Aspergillus oryzae.

    2016.7 

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    Event date: 2016.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Bacterial α-L-rhamnosidases show a dual rhamnose- and L-mannose-hydrolyzing activity.

    第53回化学関連支部合同九州大会  2016.7 

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    Event date: 2016.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • Efficient transfer of oligosaccharides onto proteins by combined use of Coprinus cinerea endoglycosidase and synthetic sialo-complex-type sugar oxazoline.

    Huang Y, Eshima Y, Kinoshita T, Sumiyoshi W, Nakakita S, Higuchi Y, Takegawa K

    2016.7 

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    Event date: 2016.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 糸状菌Aspergillus nidulansの糖代謝に関連するβ-D-ガラクトフラノシダーゼの機能解析

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 竹川 薫

    第53回化学関連支部合同九州大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombe を宿主とした異種糖タンパク質の分泌生産

    福谷早紀, 陶山明子, 樋口 裕次郎, 竹川 薫

    第53回化学関連支部合同九州大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送に重要なVps18pの機能解析と相互作用するタンパク質の探索

    大久保皓平, 落石悟, 樋口 裕次郎, 竹川 薫

    第53回化学関連支部合同九州大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおける推定AAA ATPase AipAと相互作用する因子の解析

    柿本健一, 竹川 薫, 樋口 裕次郎

    日本生化学会九州支部例会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • 糸状菌Aspergillus nidulansの糖代謝に関連するβ-D-ガラクトフラノシダーゼの機能解析

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 後藤正利, 竹川 薫

    日本生化学会九州支部例会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombe を宿主とした異種糖タンパク質の分泌生産

    福谷早紀, 陶山明子, 樋口 裕次郎, 竹川 薫

    日本生化学会九州支部例会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送に重要なVps18pの機能解析及び相互作用するタンパク質の探索

    大久保皓平, 落石悟, 樋口 裕次郎, 竹川 薫

    日本生化学会九州支部例会  2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学   Country:Japan  

  • エンドグリコシダーゼによる糖鎖改変技術開発とそのバイオ医薬品トラスツマブへの応用

    白井 孝, 黒河内政樹, 月村 亘, 森 昌子, 大隅賢二, 戸治野真美, 高嶋 晶, 菅原州一, 弘瀬友理子, 高柳 淳, 水野真盛, 天野純子, 松田昭生, 正田晋一郎, 木下崇司, 竹川 薫, 冨田正浩

    日本農芸化学会2016年度大会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • エンドグリコシダーゼ:糖鎖の機能・構造解析用試薬からバイオ医薬品製造ツールへの変遷

    竹川 薫

    日本農芸化学会2016年度大会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • 真核微生物におけるAipA様AAA ATPaseの機能解析、日本農芸化学会2016年度大会

    樋口 裕次郎, 柿本健一, 宋 原準, 竹川 薫

    日本農芸化学会2016年度大会  2016.3 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌市   Country:Japan  

  • Research on physiological roles of early endosome motility in filamentous fungi

    Higuchi Y, Takegawa K

    Protein Trafficking and Intracellular Signaling of Plant and Fungal Cells (Progress 100: Second International Symposium)  2016.2 

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    Event date: 2016.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Biosynthesis and intracellular trafficking of galactose-containing oligosaccharides in Schizosaccharomyces pombe. Invited

    Takegawa K

    Protein Trafficking and Intracellular Signaling of Plant and Fungal Cells (Progress 100: Second International Symposium)  2016.2 

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    Event date: 2016.2

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 分裂酵母の細胞内小胞輸送経路を改変した異種タンパク質分泌生産向上株の取得

    副田大介, 竹川 薫

    微生物学の新たな発展、ゲノムから機能・実用に関する九州シンポジウム  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎sい   Country:Japan  

  • 分裂酵母における液胞局在プロテアーゼの輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    微生物学の新たな発展、ゲノムから機能・実用に関する九州シンポジウム  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎市   Country:Japan  

  • 担子菌由来のエンドグリコシダーゼを利用した均一糖鎖を持つ糖タンパク質の合成

    江島康成, 竹川 薫

    微生物学の新たな発展、ゲノムから機能・実用に関する九州シンポジウム  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎市   Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖を分解する酵素の探索と同定

    松藤仁美, 樋口 裕次郎, 竹川 薫

    微生物学の新たな発展、ゲノムから機能・実用に関する九州シンポジウム  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎市   Country:Japan  

  • Schizosaccharomyces pombeにおける2つの液胞局在セリンプロテアーゼの輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    日本生物工学会第22回九州支部宮崎大会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による異種タンパク質分泌生産向上株の創製

    副田大介, 竹川 薫

    日本生物工学会第22回九州支部宮崎大会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 担子菌由来のエンド-β-N-アセチルグルコサミニダーゼ変異体を用いた均一糖鎖含有糖タンパク質の酵素合成

    江島康成, 竹川 薫

    日本生物工学会第22回九州支部宮崎大会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • Bacillus属細菌由来ピルビン酸化ガラクトース含有糖鎖分解酵素の同定と諸性質の解析

    松藤仁美, 樋口 裕次郎, 竹川 薫

    日本生物工学会第22回九州支部宮崎大会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • コアフコース含有糖鎖を持つ糖鎖改変トラスツズマブの調製とその活性

    月村 亘, 森 昌子, 大隅賢二, 戸治野真美, 高島 晶, 菅原州一, 弘瀬有理子, 高柳 淳, 水野真盛, 天野純子, 松田昭生, 木下崇司, 竹川 薫, 黒河内政樹, 白井 孝

    第88回日本生化学会大会  2015.12 

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    Event date: 2015.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸市   Country:Japan  

  • 組換え糖タンパク質生産システムの開発とその応用 Invited

    竹川 薫

    第25回WSフォーラム  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • Aspergillus nidulansの分生子形成に関与する糖転移酵素様機能未知膜タンパク質の解析

    川満洋平, 石井千尋, 浴野圭輔, 竹川 薫, 後藤 正利, 岡 拓二

    第15回糸状菌分子生物学コンファレンス  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ルミエール府中(東京)   Country:Japan  

  • Aspergillus nidulansにおけるβ-D-ガラクトフラノシダーゼの諸性質の解析

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 後藤 正利, 竹川 薫

    第15回糸状菌分子生物学コンファレンス  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ルミエール府中(東京)   Country:Japan  

  • Aspergillus oryzaeにおけるエンドサイトーシス関連AAA ATPase AipAと相互作用する因子の解析

    柿本健一, 竹川 薫, 樋口 裕次郎

    第15回糸状菌分子生物学コンファレンス  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ルミエール府中(東京)   Country:Japan  

  • Aspergillus fumigatusの推定ガラクトフラノース転移酵素遺伝子群の機能解析

    李 秋実, 片渕由佳子, 浴野圭輔, 竹川 薫, 後藤 正利, 岡 拓二

    第15回糸状菌分子生物学コンファレンス  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ルミエール府中(東京)   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース転移酵素の機能解析

    片淵由佳子, 泉 実, 浴野圭輔, 竹川 薫, 後藤 正利, 岡 拓二

    第15回糸状菌分子生物学コンファレンス  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ルミエール府中(東京)   Country:Japan  

  • 酵素法と化学合成法を用いたガラクトフラノシド誘導体の合成

    太田 涼, 篠塚早紀, 竹川 薫, 岡 拓二, 清田洋正, 泉 実

    2015日本化学会中国四国支部大会  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  • 分裂酵母の液胞膜局在タンパク質の輸送に必須なVps18タンパク質の機能解析

    大久保皓平, 落石 悟, 竹川 薫

    第33回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • 細胞内小胞輸送経路を改変した分裂酵母株による異種タンパク質分泌生産能の向上

    副田大介, Takegawa K

    第33 回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • 出芽酵母の静置培養における硫黄含有香気性化合物の生成経路の解析

    東瀬戸俊太郎, 梶原康博, 高下秀春, Takegawa K

    第33 回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送経路の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    第33回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • 分裂酵母が生産するピルビン酸化ガラクトース含有糖鎖を分解する酵素の探索と諸性質の解析

    松藤仁美, 樋口 裕次郎, 竹川 薫

    第33回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • 分裂酵母における異種タンパク質生産条件の検討

    福谷早紀, 陶山明子, 竹川 薫

    第33回YEAST WORKSHOP  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:せとうち児島ホテル(岡山)   Country:Japan  

  • Analysis of secretion pathway of acyl-CoA binding protein AoAcb2 in Aspergillus oryzae. International conference

    Kwon HS, Kawaguchi K, Kikuta T, Kitamoto K, Takegawa K, Higuchi Y

    International Symposium on Agricultural, Food, Environment and Life Science in Asia  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Vacuolar sorting mechanisms of fission yeast serine proteinases. International conference

    Ohkubo K, Higuchi Y, Takegawa K

    International Symposium on Agricultural, Food, Environment and Life Science in Asia  2015.11 

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    Event date: 2015.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Identification and characterization of endo-β-N-acetylglucosaminidases from basidiomycete. International conference

    2015.11 

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    Event date: 2015.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Kurayoshi-city, Tottori   Country:Japan  

  • 分泌輸送小胞の膜融合促進による分裂酵母の異種タンパク質生産への影響

    竹川 薫

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus fumigatusの推定ガラクトフラノース転移酵素遺伝子群の機能解析

    李 秋実, 片淵由佳子, 浴野圭輔, 竹川 薫, 後藤 正利, 岡 拓二

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus nidulansの分生子形成に関与する糖転移酵素様機能未知膜タンパク質の解析

    川満洋平, 石井千尋, 浴野圭輔, 竹川 薫, 後藤 正利, 岡 拓二

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus nidulansのガラクトフラノース転移酵素 (GfsA) の機能解析

    片渕由佳子, 浴野圭輔, 泉 実, 竹川 薫, 後藤 正利, 岡 拓二

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖を分解する酵素の検索および同定と特性解析

    松藤仁美, 森 一樹, 田代 康介, 久原 哲, 樋口 裕次郎, 竹川 薫

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • 複合型糖鎖を遊離・転移する担子菌Coprinopsis cinerea由来のエンド-β-N-アセチルグルコサミニダーゼの諸性質の解析

    江島康成, 竹川 薫

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • 代謝改変分裂酵母によるグルコースを炭素源とした3-ヒドロキシプロピオン酸高生産

    陶山明子, 竹川 薫

    第67回日本生物工学会大会  2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • GH2ファミリー由来エキソ-β-D-ガラクトフラノシダーゼの基質特異性の解析

    松永恵美子, 八色奈央, 豊田早紀, 岡 拓二, 樋口 裕次郎, 竹川 薫

    日本農芸化学会中四国支部・西日本支部合同大会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛媛大学   Country:Japan  

  • Bacillus属細菌が生産するピルビン酸化ガラクトース含有糖鎖分解酵素の同定と諸性質の解析

    松藤仁美, 樋口 裕次郎, 竹川 薫

    日本農芸化学会中四国支部・西日本支部合同大会  2015.9 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛媛大学   Country:Japan  

  • 黄麹菌 Aspergillus oryzae における初期エンドソーム動態関連因子の解析

    都甲祐介, 竹川 薫, 樋口 裕次郎

    日本農芸化学会2016年度西日本支部大会  2016.9 

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    Event date: 2015.9 - 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:長崎大学   Country:Japan  

  • 分裂酵母における2つの液胞局在セリンプロテアーゼの輸送機構の解析

    大久保和真

    酵母遺伝学フォーラム第48回研究報告会  2015.9 

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    Event date: 2015.8 - 2015.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:広島大学   Country:Japan  

  • 代謝を改変した分裂酵母によるグルコースからの3-ヒドロキシプロピオン酸高生産

    陶山明子, 竹川 薫

    酵母遺伝学フォーラム第48回研究報告会  2015.9 

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    Event date: 2015.8 - 2015.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島大学   Country:Japan  

  • 均一糖鎖を持つトランスツズマブ(6) コアフコース含有糖鎖を持つIgGの調製

    月村 亘, 森 昌子, 黒河内政樹, 大隅賢二, 高島 晶, 高柳 淳, 水野真盛, 天野純子, 松田昭生, 木下崇司, 竹川 薫, 白井 孝

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • Endo型糖鎖の活性測定系の開発

    中北愼一, 竹川 薫, 平林 淳

    第34回日本糖質学会年会  2015.7 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • Coprinopsis cinerea由来endo-β-N-acetylglucosaminidase(Endo-CC)の基質特異性の解析

    木下崇司, 江島康成, 住吉 渉, 中北愼一, 平林 淳, 竹川 薫

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • GH2ファミリーに属するエキソ-β-D-ガラクトフラノシダーゼの特性解析

    松永恵美子, 八色奈央, 豊田早紀, 岡 拓二, 竹川 薫

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖を分解する酵素の同定と諸性質の解析

    松藤仁美, 森 一樹, 田代 康介, 久原 哲, 樋口 裕次郎, 竹川 薫

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • ピルビン酸転移酵素変異体を用いた新奇酸性複合型糖鎖の合成

    竹川 薫, 吉永 将, 頼経健一, 舘野浩章, 平林 淳, 中北愼一, 角田 佳充, 樋口 裕次郎

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • オキサゾリン化SGの安定性

    住吉 渉, 木下崇司, 内海圭一郎, 中北慎一, 竹川 薫, 平林 淳

    第34回日本糖質学会年会  2015.8 

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    Event date: 2015.7 - 2015.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母のVPS9ドメインタンパク質は協調してRab5シグナル伝達を制御する

    塚本雄太, 鍵和田 聡, 嶋津小百合, 竹川 薫, 野口哲子, 宮本昌明

    第67回日本細胞生物学会大会  2015.6 

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    Event date: 2015.6 - 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • 黄麹菌Aspergillus oryzaeにおけるAAA ATPase AipA相互作用因子の探索

    柿本健一, 竹川 薫, 樋口 裕次郎

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • バイオ医薬品製造に向けた糖鎖改変・分析技術の開発 Invited

    竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母の液胞膜局在タンパク質の輸送に必須なVps18タンパク質の機能解析

    大久保皓平, 落石 悟, 樋口 裕次郎, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • Aspergillus nidulansにおけるβ-ガラクトフラノシダーゼの生理的役割

    豊田早紀, 八色奈央, 松永恵美子, 樋口 裕次郎, 後藤 正利, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母における異種糖タンパク質の分泌生産条件の検討

    福谷早紀, 陶山明子, 樋口 裕次郎, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖の分解に必要な酵素の同定と諸性質の解析

    松藤仁美, 森 一樹, 田代 康介, 久原 哲, 樋口 裕次郎, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母糖鎖中のピルビン酸化ガラクトースの生合成に関与するタンパク質の機能解析

    庭山智史, 樋口 裕次郎, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母の液胞局在セリンプロテアーゼの液胞への輸送シグナルと輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    第52回化学関連支部合同九州大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • Neo-human-type oligosaccharide that imitates sialylation developed by structural analysis of fission yeast pyruvyltransferase Pvg1p. International conference

    Takegawa K, Yoshinaga S, Yoritsune K, Tateno H, Hirabayashi J, Nakakita S, Kanekiyo M, Kakuta Y, Higuchi Y

    8th International Fission Yeast Meeting  2015.6 

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    Event date: 2015.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Analysis of vacuolar targeting signal and sorting pathways of fission yeast serine proteinases International conference

    Okubo K, Higuchi Y, Takegawa K

    8th International Fission Yeast Meeting  2015.6 

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    Event date: 2015.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Two VPS9 proteins cooperate in the regulation of Rab5 signaling in fission yeast. International conference

    Tsukamoto Y, Kagiwada S, Shimazu S, Takegawa K, Noguchi T, Miyamoto M

    8th International Fission Yeast Meeting  2015.6 

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    Event date: 2015.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant fission yeast strains. International conference

    Suyama A, Takegawa K

    8th International Fission Yeast Meeting  2015.6 

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    Event date: 2015.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖は分解できるのか Invited

    竹川 薫

    比較グライコーム研究会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 代謝経路を改変した分裂酵母による3-ヒドロキシプロピオン酸の高生産

    陶山明子, 竹川 薫

    日本生化学会九州支部例会  2015.5 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母における液胞局在セリンプロテアーゼの輸送機構の解析

    大久保和真, 樋口 裕次郎, 竹川 薫

    日本生化学会九州支部例会  2015.5 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母におけるピルビン酸化ガラクトースの生合成に関与するタンパク質の機能解析

    庭山智史, 樋口 裕次郎, 竹川 薫

    日本生化学会九州支部例会  2015.5 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • ピルビン酸化ガラクトース含有糖鎖を分解する酵素の同定と諸性質の解析

    松藤仁美, 森 一樹, 田代 康介, 久原 哲, 樋口 裕次郎, 竹川 薫

    日本生化学会九州支部例会  2015.5 

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    Event date: 2015.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 糖タンパク質を分解するための微生物の戦略 Invited

    竹川 薫

    エンドグリコシダーゼ会議  2015.4 

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    Event date: 2015.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:弘前大学   Country:Japan  

  • 植物病原性糸状菌Ustilago maydisの感染時における初期エンドソーム動態の生理学的意義

    樋口 裕次郎, Gero Steinberg, 竹川 薫

    日本農芸化学会2015年岡山大会  2015.3 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  • 分裂酵母のゲノム情報を活用した染色体組込み型異種タンパク質生産系の構築

    藤木真優, アリムジャン イディリス, 竹川 薫

    日本農芸化学会2015年岡山大会  2015.3 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  • 分裂酵母プロテアーゼ遺伝子多重破壊株による3-ヒドロキシプロピオン酸の高生産

    陶山明子, 漆原正浩, 竹川 薫

    日本農芸化学会2015年岡山大会  2015.3 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース転移酵素の機能解析

    片淵由佳子, 篠塚早紀, 泉 実, 浴野圭輔, 竹川 薫, 後藤 正利, 野村善幸, 岡 拓二

    日本農芸化学会2015年岡山大会  2015.3 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース転移酵素の機能解析

    片渕由佳子, 篠塚早紀, 泉 実, 浴野圭輔, 竹川 薫, 後藤 正利, 野村善幸, 岡 拓二

    第14回糸状菌コンファレンス  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  • 白麹菌における推定転写制御因子NosAとRosAの解析

    二神泰基, 田代智史, 梶原康博, 高下秀春, 竹川 薫, 玉置尚徳, 後藤 正利

    第14回糸状菌コンファレンス  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  • 分裂酵母のゴルジ体膜に局在するロンボイド型プロテアーゼの機能解析

    野村勇太, 東 玲那, 渋谷大介, 竹川 薫, 田淵光昭, 田中直孝

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 植物病原菌Ustilago maydisにおける初期エンドソーム動態の意義

    樋口 裕次郎, Gero Steinberg, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • フマル酸分泌生産能を向上させた分裂酵母の分子育種

    陶山明子, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母におけるTCA回路関連遺伝子破壊株の有機酸生産への影響

    前田祐華, 陶山明子, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母における小胞体関連分解機構に関与するタンパク質の機能解析

    藤崎 響, 向山博幸, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母における液胞膜タンパク質の輸送におけるVps18pの機能解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母のトランスポゾン様遺伝子配列を利用した新たな異種タンパク質生産系の構築

    藤木真優, アリムジャン イディリス, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母糖鎖合成欠損株の細胞壁安定化に機能するGPIアンカー型タンパク質

    桜井雄希, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母におけるアルカリストレスに応答するCOMTホモログ遺伝子の発現解析

    森 暁平, 富永陽大, 樋口 裕次郎, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 分裂酵母におけるAAA ATPaseの機能解析

    宋 原準, 樋口 裕次郎, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 酵母の嫌気性培養における揮発性含硫香気性化合物の生成経路の解析

    東瀬戸俊太郎, 梶原康博, 高下秀春, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による効率的な異種タンパク質分泌生産

    副田大介, 八木聖史, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombeにおける液胞局在セリンプロテアーゼの輸送機構の解析

    大久保和真, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • 分裂酵母N-結合型糖鎖のピルビン酸化ガラクトース生合成に関わるタンパク質の機能解析

    庭山智史, 頼経健一, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • Aspergillus属糸状菌のガラクトフラノース含有糖鎖の代謝に関わるβ-D-ガラクトフラノシダーゼの諸性質の解析

    八色奈央, 松永恵美子, 小野健太郎, 竹川 薫

    第32回Yeast Workshop  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ビュー・ポートくれ (広島県)   Country:Japan  

  • ビールの泡持ちを低下させる液胞プロテアーゼPrAと関連するタンパク質の解析

    佐伯 圭, 陶山明子, 竹川 薫, 佐藤雅英, 執行達朗

    2014年度第24回BCOJ年次大会  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:星陵会館(東京都)   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース含有糖鎖の代謝に関わる酵素の解析

    八色奈央, 松永恵美子, 小野健太郎, 樋口 裕次郎, 後藤 正利, 竹川 薫

    第6回日本醸造学会若手シンポジウム  2014.10 

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    Event date: 2014.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北とぴあ つつじホール   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース転移酵素の機能解析

    片淵由佳子, 浴野圭輔, 竹川 薫, 後藤 正利, 野村善幸, 岡 拓二

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による異種タンパク質分泌生産への影響

    副田大介, 八木聖史, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母の代謝改変がクエン酸蓄積におよぼす影響

    前田祐華, 陶山明子, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母における液胞膜タンパク質の輸送に関わるVps18pの機能解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Asperigllus nidulansゲノム中に存在するα-L-アラビノフラノシダーゼとβ-D-ガラクトフラノシダーゼの諸性質の比較

    八色奈央, 松永恵美子, 小野健太郎, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • ピルビン酸転移酵素変異体を用いたピルビン酸含有N-結合型糖鎖の酵素合成

    吉永 将, 頼経健一, 中北慎一, 舘野浩章, 平林 淳, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母のゲノム中に複数存在するトランスポゾン様遺伝子配列を利用した異種タンパク質生産系の構築

    藤木真優, アリムジャン イディリス, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母糖鎖合成欠損株の生育向上に機能するGPIアンカー型タンパク質の機能解析

    桜井雄希, 竹川 薫

    平成26年度日本農芸化学会西日本支部大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Asperigllus nidulansゲノム中に存在するα-L-アラビノフラノシダーゼ関連酵素の諸性質の解析

    八色 奈央, 松永 恵美子, 小野 健太郎, 竹川 薫

    第66回日本生物工学会大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  • 白麴菌Aspergillus kawachiiのクエン酸高生産に関わる遺伝子の探索

    後藤 正利, 田代智史, 二神泰基, 竹川 薫, 梶原康博, 高下秀春

    第66回日本生物工学会大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  • 分裂酵母のゲノム中に複数存在するトランスポゾン様遺伝子配列を利用した異種タンパク質生産系の構築

    藤木真優, アリムジャン イディリス, 竹川 薫

    第66回日本生物工学会大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  • Streptomyces属放線菌が生産する新規β-D-ガラクトフラノシダーゼの遺伝子同定と諸性質の解析

    松永恵美子, 八色奈央, 岡 拓二, 竹川 薫

    第66回日本生物工学会大会  2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  • 担子菌類ゲノムに存在するエンド-β-N-アセチルグルコサミニダーゼの諸性質の解析

    江島康成, 竹川 薫

    第33回日本糖質学会年会  2014.8 

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    Event date: 2014.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • Streptomyces属が生産するβ-D-ガラクトフラノシダーゼの諸性質の解析

    松永恵美子, 八色奈央, 岡 拓二, 竹川 薫

    第33回日本糖質学会年会  2014.8 

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    Event date: 2014.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • Analysis of beer form stability-affecting vacuolar protease PrA and its relevant proteins. International conference

    Kei Saeki, Akiko Suyama, Kaoru Takegawa, Masahide Sato, Tatsuro Shigyo

    2014 ASBC Annual Meeting  2014.6 

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    Event date: 2014.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  • 分裂酵母におけるエタノール非生産株の諸性質の解析

    副田 大介, 陶山 明子, 竹川 薫

    平成26年度日本生化学会九州支部例会  2014.5 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 担子菌Coprinopsis cinerea由来エンド-β-N-アセチルグルコサミニダーゼの諸性質の解析

    江島康成, 竹川 薫

    平成26年度日本生化学会九州支部例会  2014.5 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • Streptomyces属放線菌から産生されたβ-D-ガラクトフラノシダーゼの諸性質の解析

    松永恵美子, 八色奈央, 岡 拓二, 竹川 薫

    平成26年度日本生化学会九州支部例会  2014.5 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母におけるフマル酸排出トランスポーターの探索

    陶山明子, 竹川 薫

    平成26年度日本生化学会九州支部例会  2014.5 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 出芽酵母の静置培養における揮発性含硫香気性化合物の生成経路の解析

    東瀬戸俊太郎, 梶原康博, 高下秀春, 竹川 薫

    平成26年度日本生化学会九州支部例会  2014.5 

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    Event date: 2014.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • Aspergillus nidulansのGfsAはO-グリカンのGalfを合成する糖転移酵素である

    岡 拓二, 二神泰基, 浴野圭輔, 竹川 薫, 後藤正利, 野村善幸

    日本農芸化学会2014年度大会  2014.3 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学生田キャンパス(神奈川県川崎市)   Country:Japan  

  • ビールの泡持ちを低下させる酵母液胞プロテアーゼPrAと関連タンパク質の解析

    佐伯 圭, 陶山明子, 竹川 薫, 佐藤雅英, 執行達朗

    日本農芸化学会2014年度大会  2014.3 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学生田キャンパス(神奈川県川崎市)   Country:Japan  

  • 白麹菌Aspergillus kawachiiのクエン酸高生産に関わる遺伝子の探索

    田代智史, 二神泰基, 森一樹, 和田正太郎, 田代康介, 久原哲, 梶原康博, 高下秀春, 竹川薫, 後藤正利

    日本農芸化学会2014年度大会  2014.3 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学生田キャンパス(神奈川県川崎市)   Country:Japan  

  • 分裂酵母ロンボイドプロテアーゼの役割と切断機構の解析

    東 玲那, 渋谷大介, 竹川 薫, 田淵光昭, 田中直孝

    日本農芸化学会2014年度大会  2014.3 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:明治大学生田キャンパス(神奈川県川崎市)   Country:Japan  

  • 分裂酵母のゴルジ体における糖鎖へのピルビン酸付加機構の解析 Invited

    竹川 薫

    第二回オルガネラホメオスタシス研究センター研究発表会  2014.2 

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    Event date: 2014.2 - 2013.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 白麹菌Aspergillus kawachiiの糖質加水分解酵素の網羅的機能解析

    平嶋宏樹, 山下彩夏, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Aspergillus nidulansにおけるugeB遺伝子の機能解析

    田中麻左人, 二神泰基, 浴野圭輔, 竹川 薫, 後藤正利, 野村善幸, 岡 拓二

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母のアルカリストレス応答に関わるCOMTホモログ遺伝子の発現解析

    富永陽大, 竹川 薫

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による異種タンパク質分泌生産能の向上

    八木聖史, 竹川 薫

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 担子菌Coprinopsis cinereaゲノムに存在するエンド-β-N-アセチルグルコサミニダーゼの諸性質の解析

    江島康成, 竹川 薫

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Streptomyces属放線菌が生産するガラクトフラノース特異的なβ-D-ガラクトフラノシダーゼ遺伝子の同定及び諸性質の解析

    松永恵美子, 八色奈央, 森 一樹, 田代康介, 久原 哲, 竹川 薫

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 白麹菌Aspergillus kawachiiにおける推定有機酸トランスポーターの機能解析

    田代智史, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 黄麹菌Aspergillus oryzaeの細胞壁ストレス耐性に関わる機能未知遺伝子の解析

    徳永奈央, 妹尾史子, 二神泰基, 竹川 薫, 岩下和裕, 後藤正利

    第20回日本生物工学会九州支部佐賀大会  2013.12 

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    Event date: 2013.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • Aspergillus nidulansのGsfAは0-グリカンのGalfを合成する糖転移酵素である

    元松 遥, 二神泰基, 浴野圭輔, 竹川 薫, 後藤正利, 野村善幸, 岡 拓二

    第13回糸状菌分子生物学コンファレンス  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:つくば国際会議場   Country:Japan  

  • Aspergillus oryzaeの細胞壁ストレス耐性に関わる機能未知遺伝子の解析

    徳永奈央, 妹尾史子, 二神泰基, 竹川 薫, 岩下和裕, 後藤正利

    第13回糸状菌分子生物学コンファレンス  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:つくば国際会議場   Country:Japan  

  • 分裂酵母のカテコール-O-メチルトランスフェラーゼホモログタンパク質の酵素活性の解析

    中山倫子, 富永陽大, 山田直隆, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 過剰発現により分裂酵母糖鎖合成欠損株の生育を回復させるpwp1+遺伝子の機能解析

    桜井雄希, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による生育への影響と異種タンパク質分泌生産能の向

    八木聖史, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 糸状菌のガラクトフラノース特異的なβ-D-ガラクトフラノシダーゼ遺伝子の同定と諸性質の解析

    八色奈央, 松永恵美子, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母のアルカリストレスによって高発現するCOMTホモログ遺伝子のプロモーター領域の解析

    富永陽大, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母における3-ヒドロキシプロピオン酸耐性機構の解析

    前田祐華, 陶山明子, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母のエタノール非生産株の代謝改変が生育に及ぼす影響

    副田大介, 陶山明子, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母由来ピルビン酸転移酵素Pvg1pの基質特異性の改変

    吉永 将, 頼経健一, 兼清美帆, 角田佳充, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母における小胞体関連分解機構の解析

    藤崎 響, 向山博幸, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母のゲノム中に複数存在するトランスポゾン様遺伝子配列を利用した異種タンパク質生産系の構築

    藤木真優, アリムジャン・イディリス, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母の静置培養における含硫アミノ酸の代謝経路の解析

    東瀬戸俊太郎, 久保田健夫, 梶原康博, 高下秀春, 竹川 薫

    第31回Yeast Workshop  2013.11 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 白麹菌Aspergillus kawachiiにおける推定有機酸トランスポーターの機能解析

    田代智史, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    第5回日本醸造学会若手シンポジウム  2013.10 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北とぴあ(東京都北区)   Country:Japan  

  • 白麹菌Aspergillus kawachii の糖質加水分解酵素の網羅的機能解析

    平嶋宏樹, 山下彩夏, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    第5回日本醸造学会若手シンポジウム  2013.10 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北とぴあ(東京都北区)   Country:Japan  

  • 分裂酵母の液胞膜局在複数膜貫通タンパク質の輸送に関わるVps18pの解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    第46回酵母遺伝学フォーラム研究報告会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東北学院大学土樋キャンパス   Country:Japan  

  • 分裂酵母のアルカリ条件下で発現が上昇するCOMTホモログ遺伝子の解析

    富永陽大, 久保田健夫, 竹川 薫

    第46回酵母遺伝学フォーラム研究報告会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東北学院大学土樋キャンパス   Country:Japan  

  • 分裂酵母の糖鎖合成欠損株の生育低下を相補するpwp1+遺伝子の機能解析

    桜井雄希, 竹川 薫

    第46回酵母遺伝学フォーラム研究報告会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東北学院大学土樋キャンパス   Country:Japan  

  • 分裂酵母の有機酸排出に関与するトランスポーターの探索

    陶山明子, 竹川 薫

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • ガラクトフラノシド誘導体の合成と酵素加水分解

    篠塚早紀, 松本唯希, 中村健太郎, 八色奈央, 松永恵美子, 竹川 薫, 中島修平, 泉 実

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • 分裂酵母のピルビン酸転移酵素を利用したピルビン酸含有複合型糖鎖の合成

    吉永 将, 頼経健一, 竹川 薫

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • ガラクトフラノース特異的なβ-D-ガラクトフラノシダーゼ遺伝子の同定と諸性質の解析

    八色奈央, 松永恵美子, 篠塚早紀, 泉 実, 竹川 薫

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • 分裂酵母ゲノムに存在するトランスポゾン様遺伝子配列を利用した異種タンパク質生産系の構築

    藤木真優, アリムジャン・イディリス, 竹川 薫

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送と液胞融合に関与するSNAREタンパク質の機能解析

    八木聖史, 細見 昭, 竹川 薫

    日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:県立広島大学   Country:Japan  

  • ガラクトース特異的なピルビン酸転移活性を持つ分裂酵母Pvg1pの諸性質の解析

    竹川 薫, 頼経健一, 兼清美帆, 大橋貴生, 吉永 将, 角田佳充

    第32回日本糖質学会年会  2013.8 

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    Event date: 2013.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪市   Country:Japan  

  • 放線菌が生産するβ-D-ガラクトフラノシダーゼ遺伝子の同定

    松永恵美子, 八色奈央, 森 一樹, 田代康介, 久原 哲, 竹川 薫

    第32回日本糖質学会年会  2013.8 

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    Event date: 2013.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:大阪市   Country:Japan  

  • 細胞壁ストレス耐性に関わる黄麹菌の機能未知遺伝子の機能解析

    徳永奈央, 妹尾史子, 二神泰基, 竹川 薫, 岩下和裕, 後藤正利

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • Streptomyces属放線菌が生産するガラクトフラノース特異的なβ-D-ガラクトフラノシダーゼの諸性質の解析

    松永恵美子, 八色奈央, 森 一樹, 田代康介, 久原 哲, 竹川 薫

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 分裂酵母のアルカリ条件下で高発現するCOMTホモログ遺伝子の解析

    富永陽大, 久保田健夫, 竹川 薫

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 代謝経路を改変した分裂酵母によるフマル酸の分泌生産

    陶山明子, 竹川 薫

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 分裂酵母のSNARE遺伝子の過剰発現による異種タンパク質生産への影響

    八木聖史, 細見 昭, 竹川 薫

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 白麹菌Aspergillus kawachiiの糖質加水分解酵素の網羅的機能解析

    山下彩夏, 平嶋宏樹, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 白麹菌における遺伝子工学実験宿主の開発

    田代智史, 二神泰基, 梶原康博, 高下秀春, 竹川 薫, 後藤正利

    生物工学会若手セミナー  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:シーガイヤ(宮崎市)   Country:Japan  

  • 分裂酵母のカテコール O-メチルトランスフェラーゼホモログタンパク質Cmt1およびCmt2の酵素活性の解析

    中山倫子, 富永陽大, 山田直隆, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母ピルビン酸転移酵素Pvg1pの触媒活性に関与するアミノ酸の同定

    吉永 将, 頼経健一, 兼清美帆, 角田佳充, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 過剰発現により分裂酵母の糖鎖合成欠損株の生育が回復する遺伝子の取得と解析

    桜井雄希, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母における小胞体可溶性タンパク質の局在に必須なC末端配列の解析

    藤崎 響, 向山博幸, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 非必須遺伝子を大規模に削除した分裂酵母株の創製と物質生産への利用

    藤木真優, 佐々木真弓, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • Streptomyces属放線菌が生産するβ-D-ガラクトフラノシダーゼの諸性質の解析

    八色奈央, 松永恵美子, 森 一樹, 田代康介, 久原 哲, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母の液胞膜タンパク質の輸送に重要なVps18タンパク質の機能解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    第50回化学関連支部合同九州大会  2013.7 

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    Event date: 2013.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州市   Country:Japan  

  • Isolation of multicopy suppressors of the temperature-sensitive phenotype of the och1 mutation in Schizosaccharomyces pombe. International conference

    Yuki Sakurai, Kaoru Takegawa

    The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology.  2013.7 

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    Event date: 2013.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Galactose-specific recognition system in fission yeast. Invited International conference

    Kaoru Takegawa

    The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology.  2013.7 

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    Event date: 2013.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Identification and characterization of galactofuranoside-specific beta-D-galactofuranosidase from Streptomyces sp. International conference

    Nao Yairo, Emiko Matsunaga, Kazuki Mori, Kousuke Tashiro, Satoru Kuhara, Kaoru Takegawa

    The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology.  2013.7 

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    Event date: 2013.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Identification of amino acid residues essential for the activity of fission yeast puryvyltransferase Pvg1p. International conference

    Sho Yoshinaga, Ken-ichi Yoritsune, Kaoru Takegawa

    The 3rd Austria/Japan Seminar on Comparative and Developmental Glycobiology.  2013.7 

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    Event date: 2013.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母ピルビン酸転移酵素Pvg1pの構造と糖鎖工学への応用

    竹川 薫

    平成25年度比較グライコーム研究会  2013.6 

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    Event date: 2013.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • 分裂酵母の糖鎖合成欠損株の生育向上に寄与する遺伝子の探索

    桜井雄希, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母ピルビン酸転移酵素Pvg1pの活性に重要なアミノ酸の同定

    吉永 将, 頼経健一, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母における小胞体可溶性タンパク質の局在化シグナルの解析

    藤崎 響, 向山博幸, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母の非必須遺伝子を大規模に削除した株の創製と諸性質の解析

    藤木真優, 佐々木真弓, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 放線菌が生産するβ-D-ガラクトフラノシダーゼの諸性質の解析

    八色奈央, 松永恵美子, 森 一樹, 田代康介, 久原 哲, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母の液胞膜に局在する膜タンパク質の輸送機構の解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    日本生化学会九州支部例会  2013.5 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:佐賀大学   Country:Japan  

  • 分裂酵母の非性的凝集に関わるgsf1遺伝子の解析

    景山瑶子, 伊藤有紀, 大石和義, 松沢智彦, 竹川 薫, 川向 誠

    日本農芸化学会2013年度大会  2013.3 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東北大学   Country:Japan  

  • 微生物の糖鎖を介した細胞間認識機構の解明 Invited

    竹川 薫

    かがわ糖質バイオフォーラム複合糖質・糖鎖研究会  2013.1 

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    Event date: 2013.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:香川県高松市   Country:Japan  

  • Characterization of Schizosaccharomyces pombe Fnx2p and Vba2p as vacuolar amino acid transporters

    Pogsanat Pongcharoen, Miyuki Kawano-Kawada, Takayuki Sakito, Kaoru Takegawa, and Yoshimi Kakinuma

    2012.12 

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    Event date: 2012.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母の細胞凝集を抑制する転写因子Gsf1の解析

    松沢智彦、景山瑶子、川向 誠、竹川 薫

    第85回日本生化学会大会  2012.12 

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    Event date: 2012.12

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡市   Country:Japan  

  • 焼酎麹菌Aspergillus kawachiiの糖質加水分解酵素の網羅的解析

    山下彩夏、二神泰基、梶原康博、高下秀春、大森俊郎、竹川 薫、後藤正利

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • 分裂酵母エタノール誘導性プロモーターを利用した遺伝子発現システムの構築

    松沢智彦、竹川 薫

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • 分裂酵母における新規ホスホエノールピルビン酸トランスポーターの同定

    頼経健一・松沢智彦・竹川 薫

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • Aspergillus nidulansの異なるGHファミリーに属するα-L-アラビノフラノシダーゼの基質特異性の解析

    小野健太郎、松永恵美子、後藤正利、竹川 薫

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • Bacillus sp.由来α-L-ラムノシダーゼの基質特異性の解析

    森ひとみ、川原愛子、松沢智彦、田中直孝、泉 実、竹川 薫

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • Aspergillus nidulansにおける糖転移酵素PmtC基質タンパク質の同定

    上原拓磨、二神泰基、豊浦利枝子、岩下和裕、梶原康博、高下秀春、大森俊郎、竹川 薫、後藤正利

    第19回日本生物工学会九州支部大分大会  2012.12 

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    Event date: 2012.12

    Presentation type:Oral presentation (general)  

    Venue:別府大学   Country:Japan  

  • 分裂酵母のゴルジ体膜結合型転写因子の切断に関与するロンボイドプロテアーゼの機能解析

    東 玲那、渋谷大介、竹川 薫、田淵光昭、田中直孝

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母の代謝改変が有機酸蓄積に及ぼす影響

    陶山明子・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母におけるエタノール誘導性遺伝子発現システムの構築

    松沢智彦・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母におけるホスホエノールピルビン酸の新規トランスポーターの同定

    頼経健一・松沢智彦・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母のアルカリストレス応答に関わるcmt1及びcmt2遺伝子の解析

    富永陽大・久保田健夫・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現による影響

    八木聖史・細見 昭・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母における小胞体局在タンパク質の局在化シグナルの解析

    藤崎 響・向山博幸・松沢智彦・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母の液胞膜に局在する複数膜貫通タンパク質の輸送機構の解析

    落石 悟・平田晋也・中瀬 舞・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母の非必須遺伝子を大規模に削除した株の諸性質の解析

    藤木真優・佐々木真弓・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 放線菌と糸状菌が生産するβ-D-ガラクトフラノシダーゼの諸性質の比較

    八色奈央・小野健太郎・松永恵美子・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母の種々の糖鎖合成欠損変異株の糖鎖構造と生育に及ぼす影

    桜井雄希・大橋貴生・松沢智彦・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母の糖鎖へピルビン酸を転移するPvg1pの活性に重要なアミノ酸の同定

    吉永 将・頼経健一・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母のカテコール-O-メチルトランスフェラーゼ(COMT)ホモログタンパク質の諸性質の解析

    中山倫子・富永陽大・山田直隆・竹川 薫

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母ERGIC及びマーカータンパク質Emp43pの機能/構造解析

    梨子木健人、鈴木章太郎、竹川 薫、田淵光昭、田中直孝

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • 分裂酵母のゴルジ層板形成に関与するタンパク質Gmp1の機能解析

    児子隆英、工藤麻友美、松沢智彦、竹川 薫、田淵光昭、田中直孝

    第30回YEAST WORKSHOP  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:山口市   Country:Japan  

  • Aspergillus nidulansのガラクトフラナン生合成に関与する遺伝子の機能解析

    元松 遥、畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    第12回糸状菌分子生物学コンファレンス  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋市   Country:Japan  

  • Aspergillus fumigatusのガラクトフラノース転移酵素遺伝子の探索

    畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    第12回糸状菌分子生物学コンファレンス  2012.11 

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    Event date: 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋市   Country:Japan  

  • Avt3p is a vacuolar amino acid exporter involving spore formation of Schizosaccharomyces pombe. International conference

    Chardwiriyapreecha S, Manabe K, Iwaki T, Kawano-Kawada M, Sekito T., Akiyama K, Takegawa K, and Kakinuma Y

    6th International Symposium on Autophagy in Okinawa  2012.10 

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    Event date: 2012.10 - 2012.11

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母におけるエタノール誘導性プロモーターの機能解析と応用

    松沢智彦、竹川 薫

    第64回日本生物工学会大会  2012.10 

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    Event date: 2012.10

    Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  • Aspergillus nidulansにおけるMid2様タンパク質の機能解析

    二神泰基, 瀬戸和史, 梶原康博,高下秀春,大森俊郎, 竹川 薫, 後藤正利

    第64回日本生物工学会大会  2012.10 

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    Event date: 2012.10

    Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  • 白麹菌Aspergillus kawachiiの遺伝子工学実験宿主の開発

    田代智史、二神泰基, 梶原康博,高下秀春,大森俊郎, 竹川 薫, 後藤正利

    第64回日本生物工学会大会  2012.10 

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    Event date: 2012.10

    Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  • Aspergillus nidulansのガラクトフラノース転移酵素遺伝子の探索

    畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 代謝経路を改変した分裂酵母によるフマル酸の分泌生産

    陶山明子・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母の凝集を誘導する転写因子Mbx2の機能解析

    松沢智彦・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母のゴルジ体に局在する新規ホスホエノールピルビン酸トランスポーターの同定

    頼経健一・松沢智彦・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母のプロテインジスルフィドイソメラーゼを利用した異種タンパク質の分泌生産

    富永陽大・小谷哲也・向山博幸・松沢智彦・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母の異種タンパク質分泌生産に関与するSNAREタンパク質の解析

    八木聖史・細見 昭・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母の液胞タンパク質輸送に必須なVps18タンパク質の機能解析

    落石 悟・平田晋也・中瀬 舞・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母の糖鎖合成欠損変異株の糖鎖構造が生育に及ぼす影響

    桜井雄希・大橋貴生・松沢智彦・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母の大規模遺伝子削除株の諸性質の解析

    藤木真優・佐々木真弓・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母におけるタンパク質の小胞体局在化シグナルの解析

    藤崎 響・向山博幸・松沢智彦・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 放線菌が生産するβ-D-ガラクトフラノシダーゼの諸性質の解析

    八色奈央・松永恵美子・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母ピルビン酸転移酵素Pvg1pの活性に重要なアミノ酸の同定

    吉永 将・頼経健一・竹川 薫

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 焼酎麹菌Aspergillus kawachiiの糖質加水分解酵素の網羅的探索

    山下彩夏、二神泰基、梶原康博、高下秀春、大森俊郎、竹川 薫、後藤正利

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus nidulansのガラクトフラナン生合成に関与する遺伝子の機能解析

    元松 遥、畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus nidulansのガラクトフラナン生合成に関与する遺伝子の機能解析

    元松 遥、畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • Aspergillus nidulansにおけるugeB遺伝子の機能解析

    田中麻左人、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    平成24年度日本農芸化学会西日本支部及び日本栄養・食糧学会九州・沖縄支部合同大会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • 黄麹菌の機能未知遺伝子の機能同定

    徳永奈央、妹尾史子、二神泰基、竹川 薫、岩下和裕、後藤正利

    第4回日本醸造学会若手シンポジウム  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • 白麹菌Aspergillus kawachiiにおける遺伝子工学実験宿主の開発

    田代智史、二神泰基、梶原康博、高下秀春、大森俊郎、竹川 薫、後藤正利

    第4回日本醸造学会若手シンポジウム  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • 糸状菌のガラクトフラナン合成に関わるガラクトフラノース転移酵素遺伝子の同定

    岡 拓二、小町祐司、畠山信太郎、元松 遥、二神泰基、Kizjakina K、Sobrado P、浴野圭輔、竹川 薫、後藤正利、野村善幸

    第31回日本糖質学会年会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • 分裂酵母における細胞表層ガラクトース鎖および糖鎖へのピルビン酸化の生理的役割

    竹川 薫、松沢智彦、頼経健一

    第31回日本糖質学会年会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:鹿児島市   Country:Japan  

  • Genomic sequence of the shochu koji mold Aspergillus kawachii. International conference

    Futagami T, Mori K, Yamashita A, Wada S, Kajiwara Y, Takashita H, Omori T, Takegawa K, Tashiro K, Kuhara S, and Goto M

    International Biotechnology Symposium and Exhibition 2012  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Country:Korea, Republic of  

  • 分裂酵母のゴルジ体膜に局在するコイルドコイルタンパク質Gmp1の機能解析

    児子隆英、工藤真友美、松沢智彦、竹川 薫、田淵光昭、田中直孝

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • 分裂酵母における糖鎖のピルビン酸付加に関与するホスホエノールピルビン酸トランスポーターの同定

    頼経 健一、松沢 智彦、竹川 薫

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • プロテインジスルフィドイソメラーゼPdi1pを用いた分裂酵母異種タンパク質分泌生産系の構築

    富永陽大、向山博幸、松沢智彦、竹川 薫

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • 分裂酵母の非性的凝集を誘導する転写因子Mbx2の解析

    松沢智彦、竹川 薫

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • ゴルジ体膜結合型転写因子の切断に関与するロンボイドプロテアーゼの機能解析

    東 玲那、渋谷大介、竹川 薫、田淵光昭、田中直孝

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • 分裂酵母ERGIC及びマーカータンパク質Emp43pの解析

    梨子木健人、鈴木章太郎、竹川 薫、田淵光昭、田中直孝

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • 分裂酵母新規凝集素の同定と機能解析

    松沢智彦、竹川 薫

    日本生化学会生物工学若手研究者の集い  2012.6 

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    Event date: 2012.6 - 2012.7

    Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  • 分裂酵母におけるガラクトーストランスポーターの同定

    松沢智彦、竹川 薫

    日本生化学会九州支部例会  2012.5 

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    Event date: 2012.5

    Presentation type:Oral presentation (general)  

    Venue:福岡大学   Country:Japan  

  • 分裂酵母におけるプロテインジスルフィドイソメラーゼPdi1pを用いた異種タンパク質分泌生産系の構築

    富永陽大、向山博幸、松沢智彦、竹川 薫

    日本生化学会九州支部例会  2012.5 

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    Event date: 2012.5

    Presentation type:Oral presentation (general)  

    Venue:福岡大学   Country:Japan  

  • Aspergillus nidulansのガラクトフラナン生合成に関与する遺伝子の機能解析

    岡 拓二、元松遥、畠山信太郎、浴野圭輔、二神泰基、後藤正利、竹川 薫、野村善幸

    日本農芸化学会2012年度大会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:京都女子大学   Country:Japan  

  • 分裂酵母におけるグリセロール資化関連遺伝子のエタノールによる発現誘導機構の解析

    松沢智彦、竹川 薫

    日本農芸化学会2012年度大会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:京都女子大学   Country:Japan  

  • 出芽酵母における分裂酵母液胞アミノ酸トランスポーターFnx1の機能発現

    真鍋邦男、Soracom Chardwiriyapreecha、河田美幸、秋山浩一、関藤孝之、竹川 薫、柿沼喜己

    日本農芸化学会2012年度大会  2012.3 

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    Event date: 2012.3

    Presentation type:Oral presentation (general)  

    Venue:京都女子大学   Country:Japan  

  • 糖鎖構造及び機能解析用ツールとして利用可能なグリコシダーゼの探索と特性解析 Invited

    竹川 薫

    かがわ糖質バイオフォーラム第4回シンポジウム  2012.2 

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    Event date: 2012.2

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 生体内における糖鎖の多様な役割と微生物 Invited

    竹川 薫

    サイエンスカフェ  2012.2 

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    Event date: 2012.2

    Presentation type:Oral presentation (general)  

    Venue:博多駅   Country:Japan  

  • Aspergillus fumigatus のガラクトフラノース転移酵素遺伝子の探索

    畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母におけるエタノールによるグリセロール資化誘導機構の

    松沢智彦、竹川 薫

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 土壌より分離した放線菌が生産するβ-D-ガラクトフラノシダーゼの諸性質の解析

    松永恵美子、小野健太郎、竹川 薫

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母におけるエンドソーム及び液胞膜の融合に関するタンパク質の機能解析

    平田晋也、中瀬 舞、竹川 薫

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母におけるエイソソーム局在Pil1ホモログタンパク質の機能解析

    石津佳祐、中瀬 舞、竹川 薫

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • Aspergillus nidulans の分生子形成に関与する糖転移酵素様機能未知膜タンパク質の解析

    石井千尋、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • Aspergillus nidulans のガラクトマンナン生合成に関与する遺伝子の機能解析

    元松遥、畠山信太郎、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母の細胞質に局在する推定糖転移酵素Omh6pの機能解析

    駒田哲也、大橋貴生、竹川 薫

    第18回日本生物工学会九州支部福岡大会  2011.12 

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    Event date: 2011.12 - 2011.5

    Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 白麹菌Aspergillus kawachii IFO 4308株のゲノム解析

    二神泰基,森 一樹,山下彩夏,和田正太郎,梶原康博,高下秀春,大森俊郎,竹川 薫,田代康介,久原 哲,後藤正利

    糸状菌分子生物学コンファレンス  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 酵母の糖鎖認識システム Invited

    竹川 薫

    第2回糖鎖科学中部拠点プログラム講演会  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • 分裂酵母の糖鎖修飾及びBipの漏出に関与するGmn2タンパク質の機能解析

    平井啓介、岡本紘一、竹川薫、田淵光昭、田中直孝

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母におけるユビキチン依存的なアミノ酸トランスポーターの輸送メカニズムの解析

    中瀬 舞、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母において凝集素Gsf2の発現を誘導する新奇転写因子Mbx2/Pvg4の機能解析

    松沢智彦、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母の細胞質に局在する推定マンノース転移酵素Omh6pの機能解析

    駒田哲也、大橋貴生、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母におけるエンドソーム膜及び液胞膜の融合に関与するタンパク質の機能解析

    平田晋也、中瀬 舞、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母におけるPil1ホモログタンパク質の機能解析

    石津佳祐、中瀬 舞、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母の酸性糖鎖合成に関与するPvg1pの諸性質の解析

    頼経健一、松沢智彦、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • Aspergillus nidulansゲノムに存在するα-L-アラビノフラノシダーゼの基質特異性の解析

    小野健太郎、松沢智彦、竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • プロテインジスルフィドイソメラーゼPdi1pを用いた分裂酵母異種タンパク質分泌生産系の構築

    富永陽大、向山博幸、松沢智彦、 竹川 薫

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母のゴルジ体膜に局在するロンボイドプロテアーゼの機能解析

    東玲那、渋谷大介、竹川薫、田淵光昭、田中直孝

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • 分裂酵母のERGIC様コンパートメントに局在するEmp43pの解析

    梨子木健人、鈴木章太郎、竹川薫、田淵光昭、田中直孝

    第29回イーストワークショップ  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • Involvement of Vba2p and Atg22p in vacuolar amino acid compartmentalization in Schizosaccharomyces pombe.

    Sugimoto N, Iwaki T, Chardwiriyapreecha S, Shimazu M, Kawano M, Sekito T, Takegawa K, and Kakinuma Y

    2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母Ssp2キナーゼによる転写抑制因子Scr1のリン酸化とグルコース抑制解除

    松沢智彦、竹川 薫

    第84回日本生化学大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • Aspergillus 属糸状菌が有するα-L-Arabinofranosidaseのβ-D-Galactofuranosidase活性の解析

    小野健太郎、松沢智彦、大橋貴生、後藤正利、竹川 薫

    日本農芸化学会西日本・中四国支部合同大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 分裂酵母におけるグリセロール資化のエタノールによる促進機構の解析

    松沢智彦、竹川 薫

    日本農芸化学会西日本・中四国支部合同大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 酵母RNase/MRPのTAP法による調製

    服部淳子、中瀬 舞、竹川 薫、角田佳充、木村 誠

    日本農芸化学会西日本・中四国支部合同大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 分裂酵母ピルビン酸転移酵素Pvg1pの大腸菌における発現と精製酵素の諸性質の解析

    頼経健一、松沢智彦、大橋貴生、竹川 薫

    日本農芸化学会西日本・中四国支部合同大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • Aspergillus nidulansにおけるkre9ホモログ遺伝子の機能解析

    上原拓磨、瀬戸和史、二神泰基、大森俊郎、竹川 薫、後藤正利

    日本農芸化学会西日本・中四国支部合同大会  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:宮崎大学   Country:Japan  

  • 分裂酵母のゴルジ層板形成に関与するタンパク質の機能解析

    児子隆英、工藤麻友美、松沢智彦、竹川薫、田淵光昭、田中直孝

    第29回イーストワークショップ  2011.9 

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    Event date: 2011.9

    Presentation type:Oral presentation (general)  

    Venue:香川   Country:Japan  

  • Quest for the genes encoding O-glycosylated proteins that are responsible for the maintenance of cell morphology in Aspergillus nidulans. International conference

    Seto K, Futagami T, Kajiwara Y, Takashita H, Omori T, Takegawa K, and Goto M

    International Union of Microbiological Societies 2011 Congress  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母におけるアミノ酸パーミアーゼAat1の細胞内輸送機構の解析

    中瀬 舞、竹川 薫

    酵母遺伝学フォーラム第44回研究報告会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 分裂酵母のガラクトース特異的新奇凝集素Gsf2の同定と機能解析

    松沢智彦、田中直考、竹川 薫

    酵母遺伝学フォーラム第44会研究報告会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 分裂酵母のガラクトース含有糖鎖のピルビン酸付加に関与するピルビン酸転移酵素Pvg1pの機能解析

    頼経健一、松沢智彦、大橋貴生、竹川 薫

    酵母遺伝学フォーラム第44回研究報告会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 分裂酵母のERGIC様コンパートメントに局在するEmp43pの解析

    梨子木健人、鈴木 章太郎、竹川 薫、田淵 光昭、田中 直孝

    酵母遺伝学フォーラム第44回研究報告会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 分裂酵母におけるヒトハイマンノース型糖鎖の生産

    大橋貴生、藤山和仁、竹川 薫

    酵母遺伝学フォーラム第44回研究報告会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • Aspergillus 属糸状菌が生産する3つのGHファミリーに属するα-L-アラビノフラノシダーゼが有するガラクトフラノシターゼ活性の解析

    小野健太郎、松沢智彦、大橋貴生、後藤正利、竹川 薫

    第48回化学関連支部合同九州大会  2011.7 

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    Event date: 2011.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:北九州   Country:Japan  

  • 分裂酵母における糖鎖のピルビン酸化を担うピルビン酸転移酵素Pvg1pの機能解析

    頼経健一、大橋貴生、松沢智彦、竹川 薫

    第48回化学関連支部合同九州大会  2011.7 

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    Event date: 2011.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:北九州   Country:Japan  

  • Identification and analyses of VPS9 domain-containing factors in fission yeast.

    Tsukamoto Y, Matsuda T, Tsuji H, Takegawa K, and Miyamoto M

    2011.6 

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    Event date: 2011.6

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Identification of gsf2: gene essential for nonsexual folocculation and hyphal growth in Schizosaccharomyces pombe. International conference

    Matsuzawa T, Morita T, Tanaka N, and Takegawa K

    The 6th international fission yeast meeting  2011.6 

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    Event date: 2011.6

    Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • 分裂酵母における凝集素の同定と機能解析

    松沢智彦、竹川 薫

    日本生化学会九州支部例会  2011.5 

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    Event date: 2011.5

    Presentation type:Oral presentation (general)  

    Venue:久留米大学   Country:Japan  

  • ガラクトース含有糖鎖へのピルビン酸付加に関与する分裂酵母Pvg1pの機能解析

    頼経健一、大橋貴生、松沢智彦、竹川 薫

    日本生化学会九州支部例会  2011.5 

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    Event date: 2011.5

    Presentation type:Oral presentation (general)  

    Venue:久留米大学   Country:Japan  

  • Aspergillus 属糸状菌が生産するα-L-アラビノフラノシダーゼのガラクトフラノースに対する作用

    小野健太郎、松沢智彦、大橋貴生、後藤正利、竹川 薫

    日本生化学会九州支部例会  2011.5 

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    Event date: 2011.5

    Presentation type:Oral presentation (general)  

    Venue:久留米大学   Country:Japan  

  • 分裂酵母の非性的凝集を担うタンパク質の同定と解析

    松沢智彦、竹川 薫

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • 分裂酵母におけるガラクトース含有糖鎖のピルビン酸付加に関与するPvg1pの機能解析

    頼経健一、松沢智彦、大橋貴生、竹川 薫

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • Aspergillus 属におけるβ-D-Galactofuranosidase遺伝子の探索

    小野健太郎、大橋貴生、松沢智彦、後藤正利、竹川 薫

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  • Aspergillus nidulansにおけるマンノシルトランスフェラーゼ遺伝子群の機能解析

    井上大輔,大橋貴生,二神泰基,高下秀春,大森俊郎,竹川 薫,後藤正利

    第17回生物工学会九州支部大会  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • Aspergillus nidulansにおけるKre9ホモログ遺伝子の機能解析

    瀬戸和史,二神泰基,大森俊郎,竹川 薫,後藤正利

    第17回生物工学会九州支部大会  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • CoA transferase抑制による高ブタノール生産株の育種

    田中和佳,岡田啓介,吉野貞蔵,竹川 薫

    第17回生物工学会九州支部大会  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • アセトン・ブタノール菌Clostridium saccharoperbutylacetonicum N1-4における代謝転換因子の精製と機能解析

    高本 裕,吉野貞蔵,竹川 薫

    第17回生物工学会九州支部大会  2010.12 

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    Event date: 2010.12

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • シアル酸のプロトタイプとしてのピルビン酸化ガラクトースの機能、かがわ糖質バイオフォーラム Invited

    竹川 薫

    第2回機能糖鎖研究会シンポジウム  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:高松   Country:Japan  

  • ゴルジ体膜に存在するロンボイドプロテアーゼの機能解析

    渋谷大介、中井慶輔、竹川 薫、田中直孝

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母におけるガラクトース含有糖鎖のピルビン酸化に関わるpvg1遺伝子の機能解析

    頼経健一、大橋貴生、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母の非性的凝集及び偽菌糸形成に関与するタンパク質の解析

    松沢智彦、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Oral presentation (general)  

    Venue:福山   Country:Japan  

  • 分裂酵母におけるアルカリ条件下の鉄及び銅イオンの取り込みに関与する遺伝子の解析

    久保田健夫、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母における液胞膜局在アミノ酸トランスポーターの機能解析

    豊原英明、向山博幸、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母のエンドソームや液胞の膜融合に関与するタンパク質の解析

    平田晋也、中瀬 舞、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母におけるエイソソーム構成タンパク質ホモログの機能解析

    石津佳祐、中瀬 舞、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母糖鎖合成欠損株の細胞壁合成阻害剤を用いた生育改善

    駒田哲也、松沢智彦、大橋貴生、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • β-D-Galactofuranosidaseをコードする遺伝子の探索

    小野健太郎、大橋貴生、後藤正利、竹川 薫

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 分裂酵母のERGIC様コンパートメントに局在するEmp43pの解析

    梨子木健人、鈴木章太郎、竹川 薫、田中直孝

    第28回イーストワークショップ  2010.11 

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    Event date: 2010.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:福山   Country:Japan  

  • 染色体工学技術を利用した分裂酵母の有用物質生産システムの構築 Invited

    竹川 薫、東田英毅

    日本生物工学会  2010.10 

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    Event date: 2010.10

    Presentation type:Symposium, workshop panel (public)  

    Venue:宮崎   Country:Japan  

  • 分裂酵母のゴルジ層板に関与するタンパク質の解析

    舟木大伍、松沢智彦、工藤麻友美、竹川 薫、田中直孝

    日本農芸化学会中四国支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:香川大学   Country:Japan  

  • Bacillus sp.由来a-L-Rhamnosidaseの希少糖に対する作用に関する研究

    川原愛子、宮西伸光、田中直孝、泉 実、竹川 薫

    日本農芸化学会中四国支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:香川大学   Country:Japan  

  • Aspergillus nidulansにおけるマンノース転移酵素遺伝子群の機能解析

    井上大輔、大橋貴生、二神泰基、高下秀春、大森俊郎、竹川 薫、後藤正利

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 出芽酵母由来IPT1遺伝子発現による分裂酵母スフィンゴ糖脂質の組成変化と細胞内応答

    中瀬 舞、谷 元洋、竹川 薫

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母アミノ酸トランスポーターの栄養条件及び窒素飢餓条件下における細胞内局在の解析

    松永恵美子、中瀬 舞、竹川 薫

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母のガラクトース代謝関連遺伝子の発現と機能解析

    松沢智彦、竹川 薫

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母の弱アルカリ条件下における鉄及び銅イオンの取り込みに関与する遺伝子の解析

    久保田健夫、竹川 薫

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母液胞膜局在アミノ酸トランスポーターAvt3の液胞形成における役割

    豊原英明、向山博行、竹川 薫

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • Aspergillus nidulansにおける分生子形成に関する機能未知膜タンパク質の解析

    石井千尋、小町裕司、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • Aspergillus nidulansのガラクトマンナン合成に関与する遺伝子の機能解析

    小町裕司、浴野圭輔、二神泰基、竹川 薫、後藤正利、野村善幸、岡 拓二

    日本農芸化学会西日本支部大会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:崇城大学   Country:Japan  

  • 分裂酵母の分泌経路に関与するレクチン様タンパク質の解析

    梨子木健人、鈴木章太郎、竹川 薫、田中直考

    酵母遺伝学フォーラム第43回研究報告会  2010.9 

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    Event date: 2010.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:奈良   Country:Japan  

  • 分裂酵母における出芽酵母IPT遺伝子発現によるスフィンゴ糖脂質の改変とその影響

    中瀬 舞、谷 元洋、竹川 薫

    酵母遺伝学フォーラム第43回研究報告会  2010.9 

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    Event date: 2010.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:奈良   Country:Japan  

  • 分裂酵母ガラクトース資化株の取得と解析

    松沢智彦、藤田康子、田中直孝、板谷有希子、竹川 薫

    酵母遺伝学フォーラム第43回研究報告会  2010.9 

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    Event date: 2010.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:奈良   Country:Japan  

  • TSC-Rhb-TORシグナル伝達経路におけるアミノ酸透過酵素の制御機構

    中瀬由起子、村井朋香、中瀬 舞、竹川 薫、松本智裕

    酵母遺伝学フォーラム第43回研究報告会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:奈良   Country:Japan  

  • 分裂酵母の糖鎖修飾及びBipの漏出に関与するGmn2pの機能解析

    平井啓介、岡本紘一、竹川 薫、田中直考

    酵母遺伝学フォーラム第43回研究報告会  2010.9 

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    Event date: 2010.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:奈良   Country:Japan  

  • A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling International conference

    K. Hasehira, S. Nakakita, N. Miyanishi, W. Sumiyoshi, S. Hayashi, K. Takegawa, and J. Hirabayashi

    25th International Carbohydrate Symposium; ICS2010  2010.8 

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    Event date: 2010.8

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Biosynthesis and physiological role of pyruvylated galactose in fission yeast. International conference

    K. Takegawa, T. Ohashi, and N. Tanaka

    25th International Carbohydrate Symposium; ICS2010  2010.8 

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    Event date: 2010.8

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Identification and characterization of novel α1,3-galactosyltransferase genes in the fission yeast Schizosaccharomyces pombe International conference

    2010.8 

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    Event date: 2010.8

    Presentation type:Symposium, workshop panel (public)  

    Venue:Tokyo   Country:Japan  

  • Characterization of two different types of UDP-glucose/galactose 4-epimerase involved in glycosylation in fission yeast International conference

    T. Matsuzawa, Y. Nukigi, S. Suzuki, K. Takegawa, and N. Tanaka

    25th International Carbohydrate Symposium; ICS2010  2010.8 

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    Event date: 2010.8

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Mannosylinositol phosphorylceramide is a main phospholipid component and required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe International conference

    M. Nakase, M. Tani, J. Kashiwazaki, T. Nakamura, N. Tanaka, and K. Takegawa

    25th International Carbohydrate Symposium; ICS2010  2010.8 

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    Event date: 2010.8

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 糸状菌Aspergillus nidulansの糖転移酵素Pmtの基質探索

    瀬戸和史、二神泰基、竹川 薫、後藤正利

    第47回化学関連支部合同九州大会  2010.7 

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    Event date: 2010.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母のグリセロール代謝経路とその制御機構の解析

    松沢智彦、田中直考、竹川 薫

    第47回化学関連支部合同九州大会  2010.7 

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    Event date: 2010.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 真核微生物のガラクトース含有糖鎖の生合成と生理的役割 Invited

    竹川 薫

    平成22年度比較グライコーム研究会  2010.6 

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    Event date: 2010.6

    Presentation type:Oral presentation (general)  

    Venue:名古屋大学   Country:Japan  

  • 糖転移酵素のゴルジ体局在に関与する分裂酵母Vps74タンパク質の機能解析

    枌誠次郎、大橋貴生、竹川 薫

    日本生化学会九州支部例会  2010.5 

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    Event date: 2010.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母におけるC末端ER局在化シグナルとERD2ホモログ遺伝子の解析

    向山博幸、東田英毅、竹川 薫

    日本生化学会九州支部例会  2010.5 

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    Event date: 2010.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母におけるグルコース抑制解除機構の解析

    松沢智彦、藤田康子、竹川 薫

    日本生化学会九州支部例会  2010.5 

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    Event date: 2010.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島大学   Country:Japan  

  • 分裂酵母に2種類存在するUDP-ガラクトース 4-エピメラーゼのガラクトース鎖修飾への役割

    田中 直孝、鈴木 章太郎、松沢 智彦、抜木 弥生、竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母のガラクトース資化株の取得とガラクトース代謝関連遺伝子の解析

    松沢智彦、久保田健夫、藤田康子、田中直孝、板谷有希子、竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母α1,2ガラクトース転移酵素多重破壊株の糖鎖構造解析

    大橋貴生、竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母におけるアミノ酸トランスポーターの輸送メカニズムの解析

    中瀬 舞、竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母カルボキシペプチダーゼYの細胞内プロセッシングと成熟化機構の解明

    向山博幸、東田英穀、竹川 薫

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • Aspergillus nidulansの2つのUDP-グルコース/UDP-ガラクトース 4-エピメラーゼ遺伝子の機能解析

    岡 拓二、浴野 圭輔、二神 泰基、竹川 薫、後藤 正利、野村 善幸

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母のゴルジ層板で機能するタンパク質の解析

    船木大伍、松沢智彦、工藤麻友美、竹川薫、田中直孝

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 分裂酵母の糖鎖修飾及びBiPの漏出に関与するGmn2pの解析

    平井 啓介、岡本 紘一、細見 昭、竹川 薫、田中 直孝

    2010年日本農芸化学会大会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  • 希少糖に作用する微生物由来グリコシダーゼの特性解析とその応用 Invited

    竹川 薫

    第1会機能糖鎖研究会シンポジウム  2010.3 

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    Event date: 2010.3

    Presentation type:Symposium, workshop panel (public)  

    Venue:高松ホテルニューフロンティア   Country:Japan  

  • 分裂酵母ゲノム上の2つのUDP-グルコース/UDP-ガラクトース4-エピメラーゼ遺伝子の機能解析

    田中直孝、松沢智彦、竹川 薫

    第4回日本ゲノム微生物学会年会  2010.3 

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    Event date: 2010.3

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母ガラクトース資化株の取得とガラクトース代謝関連遺伝子の発現解析

    松沢智彦、竹川 薫

    第4回日本ゲノム微生物学会年会  2010.3 

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    Event date: 2010.3

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母の細胞膜タンパク質の局在化に重要なスフィンゴ脂質の機能解析

    中瀬 舞、竹川 薫

    第4回日本ゲノム微生物学会年会  2010.3 

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    Event date: 2010.3

    Presentation type:Oral presentation (general)  

    Venue:九州大学医学部   Country:Japan  

  • 希少糖等を用いた医薬品中間体及び化成品等の原料の開発 Invited

    竹川 薫

    かがわ糖質バイオフォーラム第1回シンポジウム  2010.2 

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    Event date: 2010.2

    Presentation type:Symposium, workshop panel (public)  

    Venue:かがわ国際会議場   Country:Japan  

  • Role of Avt8p in vacuolar amino compartmentalization of Schizosaccharomyces pombe.

    S. Chardwiriyapreecha, H. Mukaiyama, S. Kato, T. Sekito, T. Iwaki, K. Takegawa and Y. Kakinuma

    2009.12 

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    Event date: 2009.12

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母細胞膜局在アミノ酸トランスポーター変異体の発現により引き起こされる細胞死の解析

    中瀬 舞、竹川 薫

    第16回生物工学会九州支部大会  2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母ガラクトース転移酵素多重破壊株の諸性質の解析

    大橋貴生、竹川 薫

    第16回生物工学会九州支部大会  2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母カルボキシペプチダーゼYのプロセッシングと成熟化機構の解析

    向山博幸、竹川 薫

    第16回生物工学会九州支部大会  2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • A vacuolar transporter Avt8p for acidic amino acids in Schizosaccharomyces pombe.

    S. Chardwiriyapreecha, T. Iwaki, T. Sekito, K. Takegawa, Y. Kakinuma

    2009.11 

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    Event date: 2009.11

    Country:Japan  

  • Aspergillus nidulans のGT31ファミリーに属する機能未知糖転移酵素遺伝子の機能解析

    小町裕司、岡拓二、竹川薫、後藤正利、野村善幸

    第9回糸状菌コンファレンス  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:東京大学農学部   Country:Japan  

  • 分裂酵母のゴルジ体膜で機能するロンボイドプロテアーゼの機能解析

    渋谷大介、竹川 薫、田中直孝

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母アミノ酸トランスポーターの細胞内輸送に重要なリジン残基の解析

    中瀬 舞、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母ガラクトース資化株の諸性質の解析

    松沢智彦、田中直孝、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母糖転移酵素のゴルジ膜局在に重要なVps74タンパク質の機能解析

    枌誠次郎、大橋貴生、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母液胞膜局在アミノ酸トランスポーターの機能解析

    豊原英明、向山博幸、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母のアルカリ条件下における鉄及び銅イオンの役割について

    久保田健夫、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母Png1タンパク質の機能解析

    石津佳祐、大橋貴生、向山博幸、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母の細胞質に局在するマンノース転移酵素の機能解析

    駒田哲也、池田裕香、田中直孝、大橋貴生、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • 分裂酵母の糖類の資化性を利用した液胞タンパク質マーカーの構築

    平田晋也、大橋貴生、向山博幸、竹川 薫

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • BiPの漏出及び糖鎖修飾に関与する分裂酵母Gmn2タンパク質の解析

    平井啓介、岡本紘一、細見 昭、竹川 薫、田中直孝

    第27回イーストワークショップ  2009.11 

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    Event date: 2009.11

    Presentation type:Oral presentation (general)  

    Venue:九州工業大学(飯塚)   Country:Japan  

  • Aspergillus nidulansにおけるmannosyltransferase遺伝子群の機能解析

    井上大輔、大橋貴生、二神泰基、大森俊郎、竹川 薫、後藤正利

    2009年日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部、日本食品科学工学会西日本支部合同大会  2009.10 

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    Event date: 2009.10

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • 分裂酵母における細胞質から液胞へのタンパク質輸送経路の解析

    豊原英明、向山博幸、竹川 薫

    2009年日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部、日本食品科学工学会西日本支部合同大会  2009.10 

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    Event date: 2009.10

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • 分裂酵母のアルカリ耐性に関与する遺伝子の解析

    久保田健夫、竹川 薫

    2009年日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部、日本食品科学工学会西日本支部合同大会  2009.10 

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    Event date: 2009.10

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • Aspergillus nidulans のGT31ファミリーに属する新規糖転移酵素遺伝子の機能解析

    小町裕司、岡拓二、竹川薫、後藤正利、野村善幸

    2009年日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部、日本食品科学工学会西日本支部合同大会  2009.10 

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    Event date: 2009.10

    Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  • Biosynthesis and physiological role of pyruvylated galactose in Schizosaccharomyces pombe. International conference

    K. Takegawa, T. Ohashi, Y. Ikeda, and N. Tanaka

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Involvement of Avt8p in vacuolar amino acid uptake of fission yeast cells. International conference

    S. Chardwiriyapreecha, H. Mukaiyama, T. Sekito, T. Iwaki, K. Takegawa and Y. Kakinuma

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Country:Japan  

  • Mannosylinositol phosphorylceramide is a main phospholipid component and required for proper localization of plasma membrane proteins in fission yeast. International conference

    M. Nakase, and K. Takegawa

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Country:Japan  

  • Improvement of secretory production of heterologous proteins in fission yeast. International conference

    H. Mukaiyama, H. Tohda, and K. Takegawa

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Country:Japan  

  • Characterization of galactosyltransferase-deficient mutants in fission yeast. International conference

    T. Ohashi, H. Tohda, N. Tanaka, and K. Takegawa

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Country:Japan  

  • The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe. International conference

    T. Matsuzawa, T. Ohashi, N. Tanaka, and K. Takegawa

    The 5th International Fission Yeast Meeting  2009.10 

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    Event date: 2009.10

    Country:Japan  

  • Physiological roles of pyruvylated galactose and galactofuranoside in microbial eukaryotes. Invited

    2009.10 

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    Event date: 2009.10

    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 分裂酵母のゴルジ体膜で機能するロンボイドプロテアーゼの機能解析

    渋谷大介、竹川 薫、田中直孝

    第82回日本生化学会大会  2009.10 

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    Event date: 2009.10

    Venue:神戸ポートアイランド   Country:Japan  

  • 分裂酵母におけるガラクトース転移酵素遺伝子欠損株の諸性質の解析

    大橋貴生、東田英毅、田中直孝、竹川 薫

    第42回酵母遺伝学フォーラム研究報告会  2009.7 

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    Event date: 2009.7

    Venue:つくばノバホール   Country:Japan  

  • 分裂酵母におけるアミノ酸パーミアーゼの局在化機構とユビキチン化の解析

    中瀬 舞、竹川 薫

    第42回酵母遺伝学フォーラム研究報告会  2009.7 

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    Event date: 2009.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:つくばノバホール   Country:Japan  

  • 不均衡変異導入法による分裂酵母ガラクトース資化株の取得と解析

    松沢智彦、久保田健夫、藤田康子、田中直孝、板谷有希子、竹川 薫

    第42回酵母遺伝学フォーラム研究報告会  2009.7 

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    Event date: 2009.7

    Venue:つくばノバホール   Country:Japan  

  • 分裂酵母における異種タンパク質の分泌生産性を向上させる諸条件の検討

    向山博幸、東田英毅、竹川 薫

    第42回酵母遺伝学フォーラム研究報告会  2009.7 

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    Event date: 2009.7

    Venue:つくばノバホール   Country:Japan  

  • 分裂酵母のスフィンゴ糖脂質の機能と細胞膜アミノ酸トランスポーターの局在化機構の解析

    中瀬 舞、竹川 薫

    第46回化学関連支部合同九州大会  2009.7 

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    Event date: 2009.7

    Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場   Country:Japan  

  • 分裂酵母を用いた有用物質生産システムの開発 Invited

    竹川 薫

    日本農芸化学会西日本支部例会  2009.5 

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    Event date: 2009.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:サッポロビール日田工場   Country:Japan  

  • 分裂酵母におけるスフィンゴ糖脂質の組成と機能解析

    中瀬 舞、竹川 薫

    平成21年度日本生化学会九州支部例会  2009.5 

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    Event date: 2009.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母ガラクトース転移酵素遺伝子群の機能解析

    大橋貴生、浜 祐子、竹川 薫

    平成21年度日本生化学会九州支部例会  2009.5 

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    Event date: 2009.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母PDIホモログ遺伝子の過剰発現による異種タンパク質の分泌強化

    向山博幸、浜 祐子、竹川 薫

    平成21年度日本生化学会九州支部例会  2009.5 

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    Event date: 2009.5

    Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部   Country:Japan  

  • 分裂酵母Schizosaccharomyces pombeを宿主として発現系の工夫 —最適化をめざして—

    浜 祐子、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Symposium, workshop panel (public)  

    Venue:福岡市   Country:Japan  

  • Bacillus sp.由来α-L-ラムノシダーゼはβ-L-マンノシダーゼ活性を示す

    河原愛子、宮西伸光、田中直孝、泉 実、平林 淳、橋本 渉、村田幸作、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母におけるスフィンゴ糖脂質の機能と複数膜貫通タンパク質輸送メカニズムの解析

    中瀬 舞、田中直孝、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母のドリコールリン酸オリゴ糖生合成に関与する糖転移酵素欠損株の糖鎖構造解析

    大橋貴生、浜 祐子、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母PDIホモログ遺伝子の過剰発現によるヒト由来トランスフェリンの分泌強化

    向山博幸、 浜 祐子、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • Identification of a novel SPBC1685.07c gene involving in vacuolar amino acid transport in Schizosacchoromyces pombe

    Chardwiriyapreecha, S., Iwaki, T., Sekito, T., Takegawa, K., and Kakinuma, Y.

    2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Aspergills nidulansのUDP-ガラクトピラノースムターゼ遺伝子の機能解析

    松島 享生、岡 拓二、川森 真菜、小町 裕司、竹川 薫、後藤 正利、野村 善幸

    日本農芸化学会2009年度大会  2008.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母のゴルジマトリックスで機能するGrp1pの解析

    松沢智彦、岡本鉱一、村松慶幸、竹川 薫、田中直孝

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母のゴルジ体膜で機能するロンボイド型プロテアーゼの解析

    渋谷大介、中井慶輔、松沢智彦、竹川 薫、田中直孝

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 分裂酵母におけるα-マンノシダーゼの細胞内局在に関する研究

    田中直孝、大堀康平、浅田 歩、西村文宏、竹川 薫

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • Aspergillus nidulans O‐結合型糖鎖合成酵素の基質タンパク質の探索

    藤原絵美、古田吉史、大森俊郎、伊本亮、軸屋博之、竹川薫、後藤正利

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • アセトン・ブタノール菌ヒドロゲナーゼ低下株とその性質

    吉野貞藏、岡田啓介、藤本 瞳、後藤正利、竹川 薫、中山俊一

    日本農芸化学会2009年度大会  2009.3 

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    Event date: 2009.3

    Presentation type:Oral presentation (general)  

    Venue:福岡市   Country:Japan  

  • 希少糖等を用いた医薬品中間体及び化成品等の原料の開発 Invited

    竹川 薫

    かがわ糖質バイオフォーラム第1回シンポジウム  2009.2 

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    Event date: 2009.2

    Presentation type:Oral presentation (general)  

    Venue:香川県   Country:Japan  

  • Functional analysis of Golgi-localized rhomboid proteases in fission yeast International conference

    Tanaka, N., Shibutani, D., Nakai, K., and Takegawa, K.

    ASCB Conference  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Country:United States  

  • 分裂酵母におけるハイマンノース型糖鎖の糖タンパク質と相互作用する生体内レクチン様タンパク質の機能解析

    鈴木章太郎、松沢智彦、竹川 薫、田中直孝

    第31回日本分子生物学会年会・第81回日本生化学会大会合同大会  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  • 分裂酵母のUDP-ガラクトース生合成メカニズ厶の解析

    松沢智彦、抜木弥生、竹川 薫、田中直孝

    第31回日本分子生物学会年会・第81回日本生化学会大会合同大会  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Venue:神戸市   Country:Japan  

  • Aspergills nidulansのUDP-ガラクトピラノースムターゼの機能解析

    松島享生、岡 拓二、川森真菜、小町裕司、竹川薫、後藤正利、野村善幸

    日本生物工学会九州支部大会  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Venue:熊本県   Country:Japan  

  • クロロエチレン脱塩素化コンソーシアムにおける脱塩素化機構の解明

    福澤耕太郎、岡元冬樹、後藤正利、竹川 薫、古川謙介

    日本生物工学会九州支部大会  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Venue:熊本県   Country:Japan  

  • 酵母を用いた異種糖タンパク質分泌生産におけるグリコシレーションの役割 Invited

    竹川 薫

    第6回糖鎖科学コンソーシアム(JCGG)シンポジウム  2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  • Frontal analysis of interaction between fucose-specific lectins and L-galactose. International conference

    Teraoka, M., Miyanishi, N., Tanaka, N., Takegawa, K., and Hirabayashi, J.

    Rare Sugar Congress 2008  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Substrate specificity of a-L-rhamnosidase from Bacillus sp. toward various rare sugars International conference

    Kawahara, A., MIyanishi, N., Tanaka, N., Izumi, M., Hirabayashi, J., Hashimoto, W., Murata, K., and Takegawa, K.

    Rare Sugar Congress 2008  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • Aspergillus nidulansの糖鎖構造の解析と糖転移酵素の探索

    大久保有祐、大橋貴生、竹川 薫、後藤正利

    第8回糸状菌分子生物学コンファレンス  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:石川県   Country:Japan  

  • Aspergillus nidulansの糖転移酵素Pmtの基質タンパク質の探索

    伊本亮、松本翔、軸屋博之、藤原絵美、大森俊郎、竹川薫、後藤正利

    第8回糸状菌分子生物学コンファレンス  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:石川県   Country:Japan  

  • 分裂酵母におけるα-マンノシダーゼの細胞内局在に関する研究

    大堀康平、浅田 歩、西村文宏、竹川 薫、田中直孝

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母の糖転移酵素の局在に関与するVps74タンパク質の機能解析

    枌誠次郎、大橋貴生、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根   Country:Japan  

  • 分裂酵母における複数膜貫通タンパク質の局在化機構とスフィンゴ糖脂質の機能

    中瀬 舞、岩城知子、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母の液胞形成に関与するクラスC Vpsタンパク質の機能解析

    吉田浩二、中瀬 舞、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母の液胞膜局在アミノ酸トランスポーターの機能解析

    豊原英明、向山博幸、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母のグルコース抑制解除に関与するキナーゼの機能解析

    久保田健夫、藤田康子、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母のゴルジ体膜に局在するロンボイド型プロテアーゼの解析

    渋谷大介、中井慶輔、松沢智彦、竹川 薫、田中直孝

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母のUDP-Gal生合成経路の解析

    松沢智彦、鈴木章太郎、抜木弥生、竹川 薫、田中直孝

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母におけるGARP複合体の解析:分裂酵母におけるGARP複合体の解析

    開美裕季、斎江朋子、田中直孝、竹川 薫

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • 分裂酵母のN-結合型糖転移酵素複合体の解析

    柏矢恭兵、田中孝典、竹川 薫、田中直孝

    第26回イーストワークショップ  2008.11 

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    Event date: 2008.11

    Presentation type:Oral presentation (general)  

    Venue:島根県   Country:Japan  

  • Protein O-glycosylation affects morphology and differentiation of Aspergillus nidulans. International conference

    Goto, M., Takegawa, K., and Furukawa, K.

    13th International Biotechnology Symposium  2008.10 

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    Event date: 2008.10

    Country:China  

  • Escherichia coliによるプロパノール生産

    石橋 優、河邊唯明、後藤正利、竹川 薫、吉野貞蔵

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • Aspergillus nidulansの糖鎖転移酵素Pmtの基質タンパク質の探索

    伊本 亮、松本 翔、軸屋博之、竹川 薫、後藤正利

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 偏性嫌気性脱塩素化細菌に及ぼすクロロホルムの影響

    深城裕子、二神泰基、後藤正利、竹川 薫、古川謙介

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 分裂酵母における糖転移酵素のゴルジ体局在化機構の解析

    枌誠次郎、大橋貴生、竹川 薫

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 分裂酵母のN-結合型糖鎖生合成に関与する糖転移酵素欠損株の糖鎖構造解析

    大橋貴生、 田中直孝、 浜 祐子、竹川 薫

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 分裂酵母PDIホモログ遺伝子の過剰発現による異種タンパク質の分泌強化

    向山博幸、 浜 祐子、竹川 薫

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 分裂酵母における複数膜貫通タンパク質の細胞内輸送および局在化機構の解析

    中瀬 舞、 田中直孝、竹川 薫

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • Aspergillus nidulansの糖鎖構造解析と糖転移酵素の探索

    大久保有祐、大橋貴生、竹川 薫、後藤正利

    平成20年度日本農芸化学会西日本支部大会  2008.9 

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    Event date: 2008.9

    Venue:長崎市   Country:Japan  

  • 分裂酵母のGRIP/GRABドメインを持つゴルジマトリックスタンパク質の解析

    岡本紘一、村松慶幸、竹川 薫、田中直孝

    第41回酵母遺伝学フォーラム  2008.9 

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    Event date: 2008.9

    Venue:札幌市   Country:Japan  

  • 分裂酵母のグリセロール資化メカニズムの解析とグリセロール資化株の作製

    松沢智彦、田中直孝、竹川 薫

    第41回酵母遺伝学フォーラム  2008.9 

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    Event date: 2008.9

    Venue:札幌市   Country:Japan  

  • 分裂酵母のゴルジ体膜に局在するロンボイド型プロテアーゼの機能解析

    田中直孝、渋谷大介、中井慶輔、竹川 薫

    第41回酵母遺伝学フォーラム  2008.9 

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    Event date: 2008.9

    Venue:札幌市   Country:Japan  

  • 部位特異的変異導入によるendo-β-N-acetylglucosaminidase A(Endo-A)の糖転移効率の向上

    藤田清貴、北原兼文、菅沼俊彦、山本憲二、竹川 薫

    第28回日本糖質学会年会  2008.8 

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    Event date: 2008.8

    Venue:つくば市   Country:Japan  

  • 分裂酵母がもつ二つのRab7ホモログYpt7とYpt71が拮抗的に液胞形態を制御する

    柏崎 隼、岩城知子、竹川 薫、下田 親、中村太郎

    第60回日本細胞生物学会大会  2008.7 

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    Event date: 2008.6 - 2008.7

    Venue:横浜市   Country:Japan  

  • 分裂酵母の細胞内小胞輸送経路の解析と異種タンパク質生産への応用 Invited

    竹川 薫、浜 裕子

    第18回酵母合同シンポジウム  2008.6 

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    Event date: 2008.6

    Venue:神戸市   Country:Japan  

  • 分裂酵母のタンパク質品質管理におけるIRE1 ホモログ遺伝子の機能解析

    中井慶輔、田中直孝、竹川 薫

    第49回日本生化学会中国・四国支部例会  2008.5 

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    Event date: 2008.5

    Venue:香川   Country:Japan  

  • 分裂酵母のアミノ酸トランスポーターの細胞内動態に影響を及ぼす因子の解析

    中瀬 舞、田中直孝、竹川 薫

    第49回日本生化学会中国・四国支部例会  2008.5 

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    Event date: 2008.5

    Venue:香川   Country:Japan  

  • 分裂酵母のゴルジ体膜に存在するロンボイド型プロテアーゼの解析

    渋谷大介、竹川 薫、田中直孝

    第49回日本生化学会中国・四国支部例会  2008.5 

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    Event date: 2008.5

    Venue:香川   Country:Japan  

  • 分裂酵母の細胞質局在マンノース転移酵素の機能解析

    池田裕香、田中直孝、竹川 薫

    第49回日本生化学会中国・四国支部例会  2008.5 

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    Event date: 2008.5

    Venue:香川   Country:Japan  

  • 分裂酵母のグリセロール代謝経路に関与する遺伝子の解析

    松沢智彦、田中直孝、竹川 薫

    第49回日本生化学会中国・四国支部例会  2008.5 

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    Event date: 2008.5

    Venue:香川   Country:Japan  

  • 組換え糖タンパク質医薬品製造に有用な酵素類の探索と利用 Invited

    竹川 薫

    複合糖質・糖鎖研究会  2015.1 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:高松市   Country:Japan  

  • 酵母と糸状菌の細胞表層ガラクトース鎖の生合成と生理的役割 Invited

    竹川 薫

    未来創成微生物学寄附講座開設5周年シンポジウム  2014.4 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学農学部   Country:Japan  

  • 酵母の静置培養における揮発性含硫香気性化合物の生成経路の解析

    東瀬戸俊太郎, 梶原康博, 高下秀春, 竹川 薫

    第51回化学関連支部合同九州大会  2014.6 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • 担子菌類ゲノム中に存在するエンド-β-N-アセチルグルコサミニダーゼの諸性質の解析

    江島康成, 竹川 薫

    第51回化学関連支部合同九州大会  2014.6 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母におけるエタノール非生産株の生育能の改善

    副田 大介, 陶山明子, 竹川 薫

    第51回化学関連支部合同九州大会  2014.6 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • 分裂酵母におけるヒドロキシ酸およびその類似体の耐性機構の解析

    前田祐華, 陶山明子, 竹川 薫

    第51回化学関連支部合同九州大会  2014.6 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北九州市   Country:Japan  

  • 組換え糖タンパク質医薬品製造に必要なツール開発—新規酵素の検索と最適化— Invited

    竹川 薫

    2014.11 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:臨床研究情報センター(神戸市)   Country:Japan  

  • 鎖末端のガラクトースをめぐる真核微生物の生存戦略 Invited

    竹川 薫

    第8回多糖の未来フォーラム  2014.11 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 分裂酵母糖鎖合成欠損株の細胞壁安定化に寄与するGPIアンカー型タンパク質の機能解析

    桜井雄希, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • 2本鎖複合型糖鎖を遊離転移する担子菌由来Endo-CC1,Endo-CC2の諸性質の解析

    江島康成, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • 分裂酵母の液胞膜に局在する膜貫通タンパク質の輸送機構の解析

    落石 悟, 平田晋也, 中瀬 舞, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • ピルビン酸転移酵素変異体を用いた新奇ピルビン酸含有糖鎖の酵素合成

    吉永 将, 頼経健一, 中北慎一, 舘野浩章, 平林 淳, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • 分裂酵母の代謝改変による有機酸生産への影響

    前田祐華, 陶山明子, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • 分裂酵母のSNARE関連遺伝子の過剰発現が異種タンパク質分泌生産に与える影響

    副田大介, 八木聖史, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース含有糖鎖の代謝に関わる酵素の諸性質の解析

    八色奈央, 松永恵美子, 小野健太郎, 樋口 裕次郎, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • 分裂酵母のゲノム情報を活用した新しい異種タンパク質生産系の構築

    藤木真優, アリムジャン イディリス, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • 初期エンドソーム動態の生理学的役割に関する解析

    樋口 裕次郎, Gero Steinberg, 竹川 薫

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • 白麹菌Aspergillus kawachiiの2つの糖質加水分解酵素(GH128)の機能解析

    平嶋宏樹, 榎本亜希子, 梶原康博, 高下秀春, 竹川 薫, 後藤 正利

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • Aspergillus nidulansにおけるガラクトフラノース転移酵素の機能解析

    片渕由佳子, 篠塚早紀, 泉 実, 浴野圭輔, 竹川 薫, 後藤 正利, 野村善幸, 岡 拓二

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本大学   Country:Japan  

  • 糸状菌の推定ガラクトフラノース転移酵素遺伝子群の機能解析

    李 秋実, 片渕由佳子, 浴野圭輔, 竹川 薫, 後藤 正利, 野村善幸, 岡 拓二

    日本生物工学会第21回九州支部熊本大会  2014.12 

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    Venue:熊本大学   Country:Japan  

  • 酵母のグライコバイオロジーとグライコテクノロジーへの応用 Invited

    竹川 薫

    第6回KAGAWA機能糖鎖フォーラムシンポジウム  2008.3 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:高松市   Country:Japan  

  • 分裂酵母におけるガラクトース含有糖鎖の生合成経路と生理的機能 Invited

    竹川 薫

    シンポジウム「酵母の糖鎖生物学とその応用」  2008.2 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:つくば市   Country:Japan  

  • GH159酵素のβガラクトフラノシダーゼ活性の機能解析

    小泉 舞華, Shen Jiangyan, 田中 大, 松永 恵美子, 伊藤 文恵, 佐々木 雅人, 竹川 薫, 柴田 信之

    日本薬学会年会要旨集  2022.3  (公社)日本薬学会

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MISC

  • Guidelines for the use and interpretation of assays for monitoring autophagy.

    Klionsky DJ and 1268 authors

    Autophagy   2012.4

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Galactose-specific recognition system in the fission yeast Schizosaccharomyces pombe.

    Matsuzawa T, Ohashi T, Nakase M, Toritsune K, Takegawa K

    Trends Glycosci. Glycotechnol.   2012.3

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  • Engineering of protein secretion in yeast: strategies and impact on protein production.

    Idiris A, Tohda H, Kumagai H, Takegawa K

    Appl. Microbiol. Biotechnol.   2010.3

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  • Autophagy in the fission yeast Schizosaccharomyces pombe.

    Mukaiyama H, Nakase M, Nakamura T, Kakinuma Y, Takegawa K

    FEBS Lett.   2010.3

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  • Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system.

    Takegawa K, Tohda H, Sasaki M, Idiris A, Ohashi T, Mukaiyama H, Giga-Hama Y, Kumagai H

    Biotechnol Appl Biochem.   2009.12

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  • バイオ技術を活用した育種酵母による新たな酒造りを目指して Reviewed

    竹川 薫

    生物試料分析   2023.12

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  • 「味変」清酒のすすめ

    竹川 薫

    醸造協会誌   2022.11

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  • Enzyme assay of glycoproteins by endo-beta-N-acetylglucosaminidase. Reviewed

    #de los Reyes DM, #Bienes KM, Takegawa K

    GlycoPODv2   2021.12

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  • Author Correction: Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG (Scientific Reports, (2018), 8, 1, (246), 10.1038/s41598-017-17467-y)

    Huang Y., Higuchi Y., Kinoshita T., Mitani A., Eshima Y., Takegawa K.

    Scientific Reports   10 ( 1 )   2020.12

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    Publisher:Scientific Reports  

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    DOI: 10.1038/s41598-020-63704-2

    Scopus

  • Author Correction: Identification and characterization of a novel β-D-galactosidase that releases pyruvylated galactose (Scientific Reports, (2018), 8, 1, (12013), 10.1038/s41598-018-30508-4)

    Higuchi Y., Matsufuji H., Tanuma M., Arakawa T., Mori K., Yamada C., Shofia R., Matsunaga E., Tashiro K., Fushinobu S., Takegawa K.

    Scientific Reports   10 ( 1 )   2020.12

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    Publisher:Scientific Reports  

    This Article contains typographical errors in the Materials and Methods section under subheading ‘Accession Numbers’. “The nucleotide sequences of the ORF1119, ORF4395 and ORF4971 genes have been deposited in the DDBJ/ EMBL/GenBank under the accession nos. LC306881, LC306883 and LC306885, respectively.” should read: “The nucleotide sequences of the ORF1119, ORF4395 and ORF4971 genes have been deposited in the DDBJ/ EMBL/GenBank under the accession nos. LC317050, LC317052 and LC317053, respectively.”.

    DOI: 10.1038/s41598-020-60002-9

    Scopus

  • エンドグリコシダーゼを用いたバイオ医薬品糖タンパク質の糖鎖改変技術

    竹川 薫、樋口裕次郎

    バイオサイエンスとインダストリー   2020.5

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  • 農芸化学らしさを世界に伝える

    竹川 薫

    化学と生物   2018.7

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  • ピルビン酸含有糖鎖の生物における分布と役割

    竹川 薫, Yujiro Higuchi

    化学と生物   2017.6

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  • 九州学生本格焼酎プログラム(QSP)について

    林 圭, 伊南敏雄, 竹川 薫, 鮫島吉廣, 水光正仁

    B&I バイオサイエンスとインダストリー   2015.6

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  • Construction of a ligD disruptant for efficient gene targeting in white koji mold, aspergillus kawachii

    Tashiro S., Futagami T., Wada S., Kajiwara Y., Takashita H., Omori T., Takahashi T., Yamada O., Takegawa K., Goto M.

    Journal of General and Applied Microbiology   59 ( 3 )   257 - 260   2013.7   ISSN:00221260

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    Publisher:Journal of General and Applied Microbiology  

    DOI: 10.2323/jgam.59.257

    Scopus

  • Genome sequence of Pantoea agglomerans strain IG1

    Matsuzawa T., Mori K., Kadowaki T., Shimada M., Tashiro K., Kuhara S., Inagawa H., Soma G.i., Takegawa K.

    Journal of Bacteriology   194 ( 5 )   1258 - 1259   2012.3   ISSN:00219193

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    Publisher:Journal of Bacteriology  

    Pantoea agglomerans is a Gram-negative bacterium that grows symbiotically with various plants. Here we report the 4.8-Mb genome sequence of P. agglomerans strain IG1. The lipopolysaccharides derived from P. agglomerans IG1 have been shown to be effective in the prevention of various diseases, such as bacterial or viral infection, lifestyle-related diseases. This genome sequence represents a substantial step toward the elucidation of pathways for production of lipopolysaccharides. © 2012, American Society for Microbiology.

    DOI: 10.1128/JB.06674-11

    Scopus

  • A Myxococcus xanthus bacterial tyrosine kinase, BtkA, is required for the formation of mature spores

    Kimura Y., Yamashita S., Mori Y., Kitajima Y., Takegawa K.

    Journal of Bacteriology   193 ( 20 )   5853 - 5857   2011.10   ISSN:00219193

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    Publisher:Journal of Bacteriology  

    A Myxococcus xanthus cytoplasmic bacterial tyrosine kinase, BtkA, showed phosphorylation activity in the presence of Exo. Phosphorylated BtkA was expressed late after starvation induction and early after glycerol induction. The btkA mutant was unable to complete maturation to heat-and sonication-resistant spores under both starvation- and glycerol-induced developmental conditions. © 2011, American Society for Microbiology.

    DOI: 10.1128/JB.05750-11

    Scopus

  • 染色体工学技術を利用した分裂酵母の有用物質生産システムの構築

    竹川 薫、佐々木真弓、東田英毅

    生物工学会誌   2011.9

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  • コールドスプリングハーバー 糖鎖生物学(第2版)

    鈴木康夫、木全弘治 監訳

    丸善株式会社   2010.12

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  • Engineering of protein secretion in yeast: Strategies and impact on protein production

    Idiris A., Tohda H., Kumagai H., Takegawa K.

    Applied Microbiology and Biotechnology   86 ( 2 )   403 - 417   2010.3   ISSN:01757598

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    Publisher:Applied Microbiology and Biotechnology  

    Yeasts combine the ease of genetic manipulation and fermentation of a microorganism with the capability to secrete and modify foreign proteins according to a general eukaryotic scheme. Their rapid growth, microbiological safety, and high-density fermentation in simplified medium have a high impact particularly in the large-scale industrial production of foreign proteins, where secretory expression is important for simplifying the downstream protein purification process. However, secretory expression of heterologous proteins in yeast is often subject to several bottlenecks that limit yield. Thus, many studies on yeast secretion systems have focused on the engineering of the fermentation process, vector systems, and host strains. Recently, strain engineering by genetic modification has been the most useful and effective method for overcoming the drawbacks in yeast secretion pathways. Such an approach is now being promoted strongly by current post-genomic technology and system biology tools. However, engineering of the yeast secretion system is complicated by the involvement of many cross-reacting factors. Tight interdependence of each of these factors makes genetic modification difficult. This indicates the necessity of developing a novel systematic modification strategy for genetic engineering of the yeast secretion system. This mini-review focuses on recent strategies and their advantages for systematic engineering of yeast strains for effective protein secretion. © 2010 Springer-Verlag.

    DOI: 10.1007/s00253-010-2447-0

    Scopus

  • Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

    Takegawa K., Tohda H., Sasaki M., Idiris A., Ohashi T., Mukaiyama H., Giga-Hama Y., Kumagai H.

    Biotechnology and Applied Biochemistry   53 ( 4 )   227 - 235   2009.8   ISSN:08854513

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    Publisher:Biotechnology and Applied Biochemistry  

    The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc. pombe is considered an attractive host for the production of heterologous proteins. Expression vectors for high-level expression in Sc. pombe have been developed and many foreign proteins have been successfully expressed. However, further improvements in the protein-expressing host systems are still required for the production of heterologous proteins involved in post-translationalmodification, metabolism and intracellular trafficking. This minireview focuses on recent advances in heterologous protein production by use of engineered fission-yeast strains. © 2009 Portland Press Ltd.

    DOI: 10.1042/BA20090048

    Scopus

  • Minimum genome factory using fission yeast Schizosaccharomyces pombe, improvement of host genome for heterologous protein production.

    Giga-Hama, Y., Tohda, H., Takegawa, K. and Kumagai, H.

    Biotechnol. Appl. Biochem.   2007.12

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  • 分裂酵母のゲノム情報を活用した異種タンパク質生産

    竹川 薫

    化学と生物   2006.2

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  • 分裂酵母のポストゲノム研究-最小ゲノムセットを持ったモデル真核生物-

    東田英毅、浜 祐子、竹川 薫

    バイオサイエンスとインダストリー   2004.6

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  • Vesicle-mediated protein trasnport pathways to the vacuole in Schizosaccharomyces pombe.

    Takegawa, K., Iwaki, T., Fujita, Y., Morita, T., Hosomi, A. and Tanaka, N.

    Cell Struct. Funct.   2003.12

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  • Enzymatic synthesis of neoglycoconjugates by transglycosylation with endo-beta-N-acetylglucosaminidase A.

    Takegawa, K. and Fan, J.-Q.

    Methods Enzymol.   2003.12

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  • Biosynthetic pathway and physiological role of galactose-containing oligosaccharides in fission yeast Schizosaccharomyces pombe.

    Tanaka, N. and Takegawa, K.

    Trends Glycosci. Glycotechnol.   2001.12

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Industrial property rights

Patent   Number of applications: 2   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 日本農芸化学会

  • American Society for Microbiology

  • 酵母研究会

  • 日本生化学会

  • 日本分子生物学会

  • 日本糖質学会

  • 日本生物工学会

  • バイオインダストリー協会

  • 応用微生物学研究協議会

  • 酵母遺伝学フォーラム

  • バイオインダストリー協会

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  • 日本分子生物学会

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  • 日本生化学会

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  • 日本生物工学会

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  • 日本糖質学会

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  • 日本農芸化学会

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  • 酵母研究会

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  • 酵母遺伝学フォーラム

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  • American Society for Microbiology

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Committee Memberships

  • Organizer   Domestic

    2024.4 - 2026.3   

  • バイオインダストリー協会   常任幹事   Domestic

    2024.4 - 2026.3   

  • Organizer   Domestic

    2022.4 - 2024.3   

  • バイオインダストリー協会   常任幹事   Domestic

    2022.4 - 2024.3   

  • Executive   Domestic

    2021.6 - 2023.6   

  • 日本農芸化学会   大会担当理事   Domestic

    2021.6 - 2023.6   

  • Executive   Domestic

    2017.5 - 2019.4   

  • 日本農芸化学会   西日本支部長   Domestic

    2017.5 - 2019.4   

  • 日本農芸化学会   西日本支部長   Domestic

    2017.4 - 2019.3   

  • Steering committee member   Domestic

    2016.4 - 2020.3   

  • Councilor   Domestic

    2010.4 - 2011.3   

  • 日本農芸化学会   西日本支部評議員   Domestic

    2009.4 - Present   

  • 日本農芸化学会   学術活動強化委員会委員   Domestic

    2009.4 - Present   

  • 日本農芸化学会   西日本支部評議員  

    2009.4   

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  • 日本農芸化学会   学術活動強化委員会委員  

    2009.4   

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  • 日本生化学会   九州支部評議員   Domestic

    2008.4 - Present   

  • 日本生化学会   九州支部評議員  

    2008.4   

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  • 日本生化学会   中四国支部評議員  

    2006   

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    Committee type:Academic society

    日本生化学会

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  • 日本生物工学会   西日本支部評議員  

    2005   

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    Committee type:Academic society

    日本生物工学会

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  • Steering committee member   Domestic

    2004.4 - Present   

  • 酵母遺伝学フォーラム   運営委員  

    2004.4   

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  • 酵母遺伝学フォーラム   評議員  

    2004   

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    Committee type:Academic society

    酵母遺伝学フォーラム

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  • 日本農芸化学会   中四国支部評議員  

    2002   

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    Committee type:Academic society

    日本農芸化学会

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  • Councilor   Domestic

    1999.4 - Present   

  • 日本糖質学会   評議員  

    1999   

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    Committee type:Academic society

    日本糖質学会

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Academic Activities

  • 世話人および講演者

    大隅基礎科学創成財団小中高生と最先端研究者とのふれ合いの集い  ( 九州大学医学部百年講堂 ) 2024.1

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    Type:Competition, symposium, etc. 

    Number of participants:600

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

  • Journal of Biochemistry

    2022.4 - 2024.3

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    Type:Academic society, research group, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

  • 総務

    日本農芸化学会2019年度福岡大会  ( 九州大学 ) 2020.3

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    Type:Competition, symposium, etc. 

    Number of participants:4,000

  • 世話人

    大隅基礎科学創成財団創発セミナー  ( 福岡市 ) 2019.11

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:10

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:10

  • 座長(Chairmanship)

    日本生化学会九州支部例会  ( 九州大学医学部 ) 2014.5

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本農芸化学会2012年度大会  ( 京都 ) 2012.3

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    Type:Competition, symposium, etc. 

  • 世話人

    酵母遺伝学フォーラム第44回研究報告会  ( 九州大学医学部百年講堂 ) 2011.9

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    Type:Competition, symposium, etc. 

    Number of participants:250

  • 司会と座長

    かがわ糖質バイオフォーラム 第2回機能糖鎖研究会シンポジウム  ( 香川県高松市 ) 2010.11

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    Type:Competition, symposium, etc. 

    Number of participants:80

  • 座長(Chairmanship)

    日本農芸化学会西日本支部大会  ( 崇城大学 ) 2010.9

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    Type:Competition, symposium, etc. 

  • 司会と座長

    かがわ糖質バイオフォーラム 第1回機能糖鎖研究会シンポジウム  ( 香川県高松市 ) 2010.3

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • 司会と座長

    第7回KAGAWA機能糖鎖フォーラムシンポジウム  ( 香川県高松市 ) 2009.9 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:150

  • 第7回KAGAWA機能糖鎖フォーラムシンポジウム

    ( 香川県高松市 Japan ) 2009.9

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  • 座長(Chairmanship)

    日本生化学会  ( 香川県高松市 ) 2008.5 - Present

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    Type:Competition, symposium, etc. 

  • 日本生化学会

    ( 香川県高松市 Japan ) 2008.5

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    Type:Competition, symposium, etc. 

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  • 司会と座長

    第6回KAGAWA機能糖鎖フォーラムシンポジウム  ( 香川県高松市 ) 2008.3 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:100

  • 第6回KAGAWA機能糖鎖フォーラムシンポジウム

    ( 香川県高松市 Japan ) 2008.3

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    Type:Competition, symposium, etc. 

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  • 座長(Chairmanship)

    酵母の糖鎖生物学とその応用  ( 茨城県つくば市 ) 2008.2 - Present

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    Type:Competition, symposium, etc. 

  • 酵母の糖鎖生物学とその応用

    ( 茨城県つくば市 Japan ) 2008.2

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  • 科学技術振興機構シーズ発掘試験査読評価委員

    Role(s): Review, evaluation

    科学技術振興機構  2006.4 - 2008.3

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    Type:Scientific advice/Review 

  • 日本学術振興会科学研究費委員会専門委員

    Role(s): Review, evaluation

    日本学術振興会  2005.4 - 2007.3

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    Type:Scientific advice/Review 

  • 文部科学省科学技術・学術審議会専門委員(研究計画・評価分科会)

    Role(s): Review, evaluation

    文部科学省  2000.4 - 2002.3

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    Type:Scientific advice/Review 

  • 経済産業省産業構造審議会臨時委員

    Role(s): Review, evaluation

    経済産業省  2000.4 - 2001.3

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    Type:Scientific advice/Review 

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Research Projects

  • 油脂生産酵母の細胞表層ガラクトース含有糖鎖の構造・機能解析と物質生産への利用

    2022.5 - 2023.3

    受託研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 真核微生物細胞壁の完全なαグルカン合成に必須なアミラーゼホモログの特異性解析

    Grant number:23K23511  2022.4 - 2025.3

    科学研究費助成事業  基盤研究(B)

    竹川 薫

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    Grant type:Scientific research funding

    真核微生物である酵母には、グルカン、マンナン、キチンを主な構成成分とする強固な細胞壁が存在する。申請者は出芽酵母ゲノム中には存在しない、アミラーゼと高い相同性を示す遺伝子が分裂酵母には7遺伝子存在しており、これらの中でaah3と名付けた遺伝子破壊株では、分裂酵母細胞壁の構造に異常を示すことを見出した。そこで本研究では、これらアミラーゼホモログ遺伝子(産物)が、分裂酵母の細胞壁合成に、どのように関与しているのか、遺伝子破壊株の緒性質の解析や、基質特異性の解析などを通じて明らかにすることを目的としている。

    CiNii Research

  • 真核微生物細胞壁の完全なαグルカン合成に必須なアミラーゼホモログの特異性解析

    Grant number:22H02244  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 真核微生物の細胞表層糖鎖マーカーとしての酸性糖鎖の選別機構と生理的役割の解明

    2021.6 - 2023.6

    受託研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 酵母と酢酸菌の希少糖類を介した共生メカニズムの解析と発酵食品への利用

    2021.6 - 2022.6

    受託研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 均一糖鎖含有バイオ医薬品をワンステップで合成する技術による最適糖鎖構造の探索

    Grant number:21K19090  2021 - 2023

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

    竹川 薫

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    Authorship:Principal investigator  Grant type:Scientific research funding

    本研究ではまず、固定化脱糖鎖用ENGaseと固定化糖鎖転移用ENGaseの最適反応条件の検討を行う。さらに糖タンパク質の糖鎖の根本部分に存在するフコース残基を遊離するため、ENGaseとフコシダーゼを固定化したカラムによるGlcNAc-タンパク質の効率的生産の検討を行う。これらの条件検討により脱糖鎖&糖鎖付加が可能な固定化カラムが完成すれば、均一糖鎖への変換を実施して、種々のN-結合型糖鎖を付加した場合のバイオ医薬品(具体的には抗体IgGを用いる)の活性評価を実施する予定である。

    CiNii Research

  • ヒト皮膚マイクロバイオームのバランス維持による悪玉菌の活性抑制型皮膚用剤の高精度生産技術の開発

    2020 - 2022

    戦略的基盤技術高度化支援事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • ピルビン酸含有酸性糖鎖の生物界における分布および生合成と生理的役割の解明

    Grant number:17H03966  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 焼酎酵母の醸造特性に関与する遺伝子群の解明に関する共同研究

    2014.12 - 2016.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 分裂酵母のピルビン酸転移酵素変異体を用いた新奇糖鎖の酵素合成と糖鎖工学への応用

    Grant number:26292054  2014 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 下面ビール酵母W34株の液胞タンパク質輸送メカニズム解明の検討

    2013.4 - 2014.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 「非可食性植物由来化学品製造プロセス技術開発プロジェクト」

    2013 - 2015

    NEDO事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 均一糖鎖糖タンパク質製造用の酵素とシアリル糖鎖誘導体の大量生産方法の開発

    2013 - 2015

    戦略的基盤技術高度化支援事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 汎用有機酸製造用酵母の構築

    2012.4 - 2013.3

    共同研究

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  • 代謝機能解析及び物質生産用宿主として利用可能な分裂酵母大規模遺伝子削除株の取得

    Grant number:23651206  2011 - 2013

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • シアル酸のプロトタイプである分裂酵母ピルビン酸化ガラクトースの生合成及び機能解明

    Grant number:23380204  2011 - 2013

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 糖類の代謝経路を改変した酵母による有用物質生産系の確立 竹川 (松沢)

    2011 - 2012

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Scientific research funding

  • 化学品原料の転換・多様化を可能とする革新グリーン技術の開発:バイオマスを原料として有機酸の高効率製造方法開発とその品種展開の研究開発

    2010

    グリーン・サステナブルケミカルプロセス基盤技術開発

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 遺伝子技術をベースにした酵母の育種研究

    2010

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    Grant type:Donation

  • 分裂酵母を用いた化成品生産プラットフォームの研究開発

    2010

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    Grant type:Donation

  • 遺伝子技術をベースにした酵母の育種研究

    2009.4 - 2015.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 不均衡変異導入法による分裂酵母の宿主細胞機能の開発

    2008.7 - 2009.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 分裂酵母高性能宿主細胞創製技術の研究

    2008.4 - 2009.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 特徴ある糖質の機能を生かした健康バイオ産業の創出

    2008 - 2011

    都市エリア産学官連携促進事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 分裂酵母細胞内の分泌経路における異常糖タンパク質の認識および分解機構の解明

    Grant number:19380051  2007 - 2009

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 分裂酵母の自食作用による液胞の蛋白分解機構と液胞内アミノ酸の生理的役割の解明

    Grant number:19044029  2007 - 2008

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 微生物機能を活用した高度製造基盤技術開発—分裂酵母高性能宿主細胞創製技術の研究開発—

    2006 - 2011

    新エネルギー・産業技術総合開発機構(NEDO)

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 分裂酵母の小胞輸送におけるホスファチジルイノシトールリン酸結合蛋白質の機能解析

    2004 - 2006

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 糖鎖工学における重要なツールとしての微生物エンドグリコシダーゼの基礎から応用まで

    2004

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 宿主細胞創製技術の開発—分裂酵母MGFの創製—

    2001 - 2006

    新エネルギー・産業技術総合開発機構(NEDO)

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 分裂酵母のオルガネラ輸送シグナルを用いた異種タンパク質生産システムの構築と実用化

    2000 - 2002

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 植物の生存戦略における液胞機能の総合的理解

    1998 - 2002

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 変異酵母を利用するダイズタンパク質の分泌生産システムの構築と食品利用化

    1998 - 2000

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • エンド型グリコシダーゼの特異的な活性を利用した新しい糖鎖工学的 研究の展開

    1997 - 2000

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 分裂酵母の液胞タンパク質輸送に関与する遺伝子群の解明とその応用 に関する研究

    1997 - 1998

    科学研究費助成事業  奨励研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 植物細胞における空胞系(Vacuolar system)の動態の分子機構

    1996 - 1997

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 酵母のイノシトールリン脂質を介した分泌タンパク質輸送機構の解明

    1996

    科学研究費助成事業  奨励研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

▼display all

Class subject

  • 生命の科学B

    2023.12 - 2024.2   Winter quarter

  • 発酵化学特論

    2023.10 - 2023.12   Fall quarter

  • 発酵微生物学Ⅱ

    2023.6 - 2023.8   Summer quarter

  • 基礎生化学Ⅱ

    2023.6 - 2023.8   Summer quarter

  • Microbiology Ⅱ

    2023.6 - 2023.8   Summer quarter

  • 遺伝子組換え生物の利用と制御

    2023.4 - 2023.9   First semester

  • 発酵微生物学Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 微生物生産工学特論

    2023.4 - 2023.6   Spring quarter

  • Microbiology Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 応用生命化学発展実験

    2022.10 - 2023.3   Second semester

  • 生命の科学B

    2021.12 - 2022.2   Winter quarter

  • 発酵化学特論

    2021.10 - 2021.12   Fall quarter

  • Master's Thesis Research ⅠI

    2021.4 - 2022.3   Full year

  • 卒業研究(応用生命化学分野)

    2021.4 - 2022.3   Full year

  • 科学英語(応用生命化学分野)

    2021.4 - 2021.9   First semester

  • 発酵微生物学

    2021.4 - 2021.9   First semester

  • 応用生命化学実験

    2021.4 - 2021.9   First semester

  • Seminar in a Specified Field ⅠI

    2021.4 - 2021.6   Spring quarter

  • 微生物生産工学特論

    2021.4 - 2021.6   Spring quarter

  • Microbiology

    2021.4 - 2021.6   Spring quarter

  • 生命の科学B

    2020.12 - 2021.2   Winter quarter

  • 応用生命化学発展実験

    2020.10 - 2021.3   Second semester

  • 微生物学基礎実験(応用生命化学分野)

    2020.10 - 2021.3   Second semester

  • 発酵化学特論

    2020.10 - 2020.12   Fall quarter

  • 卒業研究(応用生命化学分野)

    2020.4 - 2021.3   Full year

  • 発酵微生物学

    2020.4 - 2020.9   First semester

  • 実地見学(応用生命化学分野)

    2020.4 - 2020.9   First semester

  • 科学英語(応用生命化学分野)

    2020.4 - 2020.9   First semester

  • 応用生命化学実験

    2020.4 - 2020.9   First semester

  • Microbiology

    2020.4 - 2020.6   Spring quarter

  • 微生物生産工学特論

    2020.4 - 2020.6   Spring quarter

  • Master's Thesis Research Ⅰ

    2020.4 - 2020.6   Spring quarter

  • Seminar in a Specified Field Ⅰ

    2020.4 - 2020.6   Spring quarter

  • 生命の科学B

    2019.12 - 2020.2   Winter quarter

  • 遺伝子組換え生物の利用と制御

    2019.10 - 2020.3   Second semester

  • 発酵化学特論

    2019.10 - 2019.12   Fall quarter

  • 修士論文

    2019.4 - 2020.3   Full year

  • Master's Thesis Research Ⅱ

    2019.4 - 2019.6   Spring quarter

  • 微生物生産工学特論

    2019.4 - 2019.6   Spring quarter

  • Seminar in a Specified Field Ⅲ

    2019.4 - 2019.6   Spring quarter

  • 生命の科学B

    2018.12 - 2019.2   Winter quarter

  • システム生物工学プロジェクト演習

    2018.12 - 2019.2   Winter quarter

  • 遺伝子組換え生物の利用と制御

    2018.10 - 2019.3   Second semester

  • システム生物工学演習第1

    2018.10 - 2019.3   Second semester

  • 分子微生物学・バイオマス資源化学特別研究第2

    2018.10 - 2019.3   Second semester

  • 微生物学基礎実験(応用生命化学分野)

    2018.10 - 2019.3   Second semester

  • 発酵化学特論

    2018.10 - 2018.12   Fall quarter

  • 卒業研究

    2018.4 - 2019.3   Full year

  • システム生物工学特別研究第一

    2018.4 - 2019.3   Full year

  • システム生物工学特別研究第一

    2018.4 - 2019.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第1

    2018.4 - 2019.3   Full year

  • 発酵微生物学

    2018.4 - 2018.9   First semester

  • 科学英語II

    2018.4 - 2018.9   First semester

  • Microbiology

    2018.4 - 2018.9   First semester

  • 微生物生産工学特論

    2018.4 - 2018.6   Spring quarter

  • 生命の科学B

    2017.12 - 2018.2   Winter quarter

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2017.10 - 2018.3   Second semester

  • 遺伝子組換え生物の利用と制御

    2017.10 - 2018.3   Second semester

  • 分子微生物学・バイオマス資源化学特別研究第1

    2017.4 - 2018.3   Full year

  • 農学入門II

    2017.4 - 2018.3   Full year

  • 卒業研究

    2017.4 - 2018.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第2

    2017.4 - 2018.3   Full year

  • 微生物生産工学特論

    2017.4 - 2017.9   First semester

  • Microbiology

    2017.4 - 2017.9   First semester

  • 微生物学基礎実験(応用生命化学分野)

    2017.4 - 2017.9   First semester

  • 発酵微生物学

    2017.4 - 2017.9   First semester

  • 科学英語II

    2017.4 - 2017.9   First semester

  • 生物化学

    2016.10 - 2017.3   Second semester

  • 分子生物学概論

    2016.10 - 2017.3   Second semester

  • 生物化学

    2015.10 - 2016.3   Second semester

  • 応用生命化学発展実験

    2015.10 - 2016.3   Second semester

  • 卒業研究

    2015.4 - 2016.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第二

    2015.4 - 2016.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第一

    2015.4 - 2016.3   Full year

  • 発酵微生物学

    2015.4 - 2015.9   First semester

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2015.4 - 2015.9   First semester

  • 微生物生産工学特論

    2015.4 - 2015.9   First semester

  • 科学英語II

    2015.4 - 2015.9   First semester

  • 科学英語I

    2014.10 - 2015.3   Second semester

  • 応用生命化学発展実験

    2014.10 - 2015.3   Second semester

  • 分子生物学概論

    2014.10 - 2015.3   Second semester

  • 生物化学

    2014.10 - 2015.3   Second semester

  • 卒業研究

    2014.4 - 2015.3   Full year

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2014.4 - 2015.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第二

    2014.4 - 2015.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第一

    2014.4 - 2015.3   Full year

  • 発酵微生物学

    2014.4 - 2014.9   First semester

  • 微生物生産工学特論

    2014.4 - 2014.9   First semester

  • 基礎化学B

    2014.4 - 2014.9   First semester

  • 科学英語II

    2014.4 - 2014.9   First semester

  • 科学英語I

    2013.10 - 2014.3   Second semester

  • 発酵化学特論

    2013.10 - 2014.3   Second semester

  • 分子生物学概論

    2013.10 - 2014.3   Second semester

  • 生物化学

    2013.10 - 2014.3   Second semester

  • 分子微生物学・バイオマス資源化学特別研究第二

    2013.4 - 2014.3   Full year

  • 応用生物科学概要

    2013.4 - 2014.3   Full year

  • 卒業研究

    2013.4 - 2014.3   Full year

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2013.4 - 2014.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第一

    2013.4 - 2014.3   Full year

  • 応用生命化学発展実験

    2013.4 - 2013.9   First semester

  • 発酵学

    2013.4 - 2013.9   First semester

  • 微生物生産工学特論

    2013.4 - 2013.9   First semester

  • 基礎化学B

    2013.4 - 2013.9   First semester

  • 科学英語II

    2013.4 - 2013.9   First semester

  • 発酵化学特論

    2012.10 - 2013.3   Second semester

  • 科学英語I

    2012.10 - 2013.3   Second semester

  • 生物化学

    2012.10 - 2013.3   Second semester

  • 分子生物学概論

    2012.10 - 2013.3   Second semester

  • 応用生物科学概要

    2012.4 - 2013.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第二

    2012.4 - 2013.3   Full year

  • 分子微生物学・バイオマス資源化学特別研究第一

    2012.4 - 2013.3   Full year

  • 分子微生物学・バイオマス資源化学プロジェクト演習

    2012.4 - 2013.3   Full year

  • 卒業研究

    2012.4 - 2013.3   Full year

  • 発酵学

    2012.4 - 2012.9   First semester

  • 応用生命化学発展実験

    2012.4 - 2012.9   First semester

  • 科学英語II

    2012.4 - 2012.9   First semester

  • 基礎化学B

    2012.4 - 2012.9   First semester

  • 微生物生産工学特論

    2012.4 - 2012.9   First semester

  • 微生物生産工学特論

    2010.10 - 2011.3   Second semester

  • 発酵学

    2010.4 - 2010.9   First semester

  • 応用微生物学特論

    2010.4 - 2010.9   First semester

  • 発酵学

    2009.4 - 2009.9   First semester

  • 応用微生物学特論

    2009.4 - 2009.9   First semester

  • 生物機能科学通論

    2009.4 - 2009.9   First semester

  • 応用生物科学概要

    2009.4 - 2009.9   First semester

  • 応用微生物学特論

    2008.4 - 2008.9   First semester

  • 発酵学

    2008.4 - 2008.9   First semester

  • 応用生物科学概要

    2008.4 - 2008.9   First semester

  • 生物機能科学通論

    2008.4 - 2008.9   First semester

▼display all

FD Participation

  • 2016.2   Role:Moderator   Title:第3回農学研究員FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2010.9   Role:Participation   Title:G30 農学部国際コースについて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2009.8   Role:Participation   Title:農学研究院部門組織再編の基本方針等について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2008.12   Role:Participation   Title:「生物資源環境科学府の組織再編」について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2008.4   Role:Participation   Title:農学系に係る研究の活性化について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2008.4   Role:Participation   Title:新任教員の研修

    Organizer:University-wide

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Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2017  名古屋大学農学研究院  Classification:Part-time lecturer  Domestic/International Classification:Overseas 

    Semester, Day Time or Duration:後期

  • 2014  鹿児島大学大学院農学研究院  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2014  放送大学  Classification:Intensive course  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:7/2-3

  • 2010  香川大学  Classification:Affiliate faculty  Domestic/International Classification:Japan 

  • 2010  石川県立大学生物資源環境学部大学院  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2009  香川大学  Classification:Affiliate faculty  Domestic/International Classification:Japan 

  • 2009  神戸大学理学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2008  徳島大学大学院薬学研究科  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2008  香川大学  Classification:Affiliate faculty  Domestic/International Classification:Japan 

  • 2006  徳島大学大学院薬学研究科  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2005  京都大学大学院農学研究科  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2002  山口大学工学部大学院  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2001  高知大学農学部大学院  Classification:Intensive course  Domestic/International Classification:Japan 

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Other educational activity and Special note

  • 2021  Class Teacher 

  • 2013  Class Teacher 

Social Activities

  • かがわ糖質バイオフォーラム第2回機能糖鎖研究会シンポジウム

    かがわ糖質バイオフォーラム  香川県高松市  2010.11

     More details

    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • かがわ糖質バイオフォーラム第1回機能糖鎖研究会シンポジウム

    かがわ糖質バイオフォーラム  香川県高松市  2010.3

     More details

    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 東筑高校「総合的な学習の時間」講師

    2009.10

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • 第7回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2008.9

     More details

    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第6回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2008.3

     More details

    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第5回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2007.10

     More details

    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第4回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2007.1

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    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第3回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2006.7

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    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第2回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2006.1

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    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第1回KAGAWA機能糖鎖フォーラムシンポジウム

    KAGAWA機能糖鎖フォーラム  香川県高松市  2005.9

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    Audience: General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

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Media Coverage

  • バイオ医薬品の効率的な開発に関する伏見製薬所との共同研究 Newspaper, magazine

    日本経済新聞(四国版)  2013.11

     More details

    バイオ医薬品の効率的な開発に関する伏見製薬所との共同研究

  • 戦略的基盤技術高度化支援事業に関する伏見製薬所との共同研究 Newspaper, magazine

    読売新聞(四国版)  2013.10

     More details

    戦略的基盤技術高度化支援事業に関する伏見製薬所との共同研究

Acceptance of Foreign Researchers, etc.

  • Bioresouce Research Center

    Acceptance period: 2019.4 - 2019.9   (Period):1 month or more

    Nationality:Pakistan

    Business entity:Private/Foundation