Updated on 2024/09/30

Information

 

写真a

 
HINO MASATO
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Assistant Professor
Faculty of Agriculture Institute of Genetic Resources（Joint Appointment）
Faculty of Agriculture Insect Science and Creative Entomology Center（Joint Appointment）
School of Agriculture Department of Bioresource and Bioenvironment（Joint Appointment）
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology（Joint Appointment）
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928024818
Profile
カイコの系統維持を行う。また、保存しているカイコを用いてカイコ遺伝子研究も行う。さらに、産業昆虫であるカイコを使用した組換えタンパク質生産にも携わる。
Homepage
External link

Degree

  • Doctor of Agriculture

Research Interests・Research Keywords

  • Research theme：Generation of novel silkworm cultured cells.

    Keyword：Silkworm, Cultured cell

    Research period： 2021.10

  • Research theme：Research on sanitary insect-borne viruses Research on sanitary insects

    Keyword：Insect, Virus

    Research period： 2020.4 - 2024.3

  • Research theme：Construction of Silkworm Artificial Chromosomes Studies on Silkworm Chromosome Structure

    Keyword：Silkworm, Artificial Chromosomes, Chromosome Structure

    Research period： 2020.4

  • Research theme：Production of useful materials using the silkworm, Bombyx mori.

    Keyword：Silkworm, Useful materials, Recombinant proteins

    Research period： 2014.9

Awards

  • 九州大学生物資源環境科学府賞

    2017.3  

  • 九州大学学生後援会学術研究賞

    2016.3   九州大学学生後援会  

  • Best Student Poster Presentation

    2015.4   International Symposium on Basic and Applied Research on Sericulture and Insect Sciences  

  • Outstanding Young Scholar Award

    2015.4   The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  

  • 九州大学農学部賞

    2015.3  

  • 九州大学山川賞

    2013.12   九州大学  

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Papers

  • Efficient and Accurate BmNPV Bacmid Editing System by Two-step Golden Gate Assembly

    Takeru Ebihara, Misaki Shibuya, Ayaka Yamaguchi, Masato Hino, Jae Man Lee, Takahiro Kusakabe, Hiroaki Mon

    Journal of Virological Methods   330   115029 - 115029   2024.12   （ ISSN:0166-0934 eISSN:1879-0984 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jviromet.2024.115029

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  • Blowflies are potential vector for avian influenza virus at enzootic area in Japan. International journal

    Ryosuke Fujita, Takuji Tachi, Masato Hino, Kosuke Nagata, Masahiro Saiki, Mizue Inumaru, Yukiko Higa, Kentaro Itokawa, Nozomi Uemura, Ryo Matsumura, Izumi Kai, Kyoko Sawabe, Mutsuo Kobayashi, Haruhiko Isawa, Takahiro Kusakabe, Kazunori Matsuo, Shinji Kasai

    Scientific reports   14 ( 1 )   10285 - 10285   2024.5   （ ISSN:2045-2322 ）

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    High pathogenicity avian influenza (HPAI) poses a significant threat to both domestic and wild birds globally. The avian influenza virus, known for environmental contamination and subsequent oral infection in birds, necessitates careful consideration of alternative introduction routes during HPAI outbreaks. This study focuses on blowflies (genus Calliphora), in particular Calliphora nigribarbis, attracted to decaying animals and feces, which migrate to lowland areas of Japan from northern or mountainous regions in early winter, coinciding with HPAI season. Our investigation aims to delineate the role of blowflies as HPAI vectors by conducting a virus prevalence survey in a wild bird HPAI-enzootic area. In December 2022, 648 Calliphora nigribarbis were collected. Influenza virus RT-PCR testing identified 14 virus-positive samples (2.2% prevalence), with the highest occurrence observed near the crane colony (14.9%). Subtyping revealed the presence of H5N1 and HxN1 in some samples. Subsequent collections in December 2023 identified one HPAI virus-positive specimen from 608 collected flies in total, underscoring the potential involvement of blowflies in HPAI transmission. Our observations suggest C. nigribarbis may acquire the HPAI virus from deceased wild birds directly or from fecal materials from infected birds, highlighting the need to add blowflies as a target of HPAI vector control.

    DOI： 10.1038/s41598-024-61026-1

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  • Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori

    Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe

    Insects   14 ( 8 )   684 - 684   2023.8   （ ISSN:2075-4450 eISSN:2075-4450 ）

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    Language：Others   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Spermatogenesis is an important process in reproduction and is conserved across species, but in Bombyx mori, it shows peculiarities, such as the maintenance of spermatogonia by apical cells and fertilization by dimorphic spermatozoa. In this study, we attempted to characterize the genes expressed in the testis of B. mori, focusing on aspects of expression patterns and gene function by transcriptome comparisons between different tissues, internal testis regions, and Drosophila melanogaster. The transcriptome analysis of 12 tissues of B. mori, including those of testis, revealed the widespread gene expression of 20,962 genes and 1705 testis-specific genes. A comparative analysis of the stem region (SR) and differentiated regions (DR) of the testis revealed 4554 and 3980 specific-enriched genes, respectively. In addition, comparisons with D. melanogaster testis transcriptome revealed homologs of 1204 SR and 389 DR specific-enriched genes that were similarly expressed in equivalent regions of Drosophila testis. Moreover, gene ontology (GO) enrichment analysis was performed for SR-specific enriched genes and DR-specific enriched genes, and the GO terms of several biological processes were enriched, confirming previous findings. This study advances our understanding of spermatogenesis in B. mori and provides an important basis for future research, filling a knowledge gap between fly and mammalian studies.

    DOI： 10.3390/insects14080684

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  • High yield production of norovirus GII.4 virus-like particles using silkworm pupae and evaluation of their protective immunogenicity. International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Shintaro Sato, Atsushi Masuda, Masahiro Taniguchi, Ryosuke Fujita, Hiroshi Ushijima, Keisuke Morimoto, Takeru Ebihara, Masato Hino, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe

    Vaccine   41 ( 3 )   766 - 777   2023.1   （ ISSN:0264-410X eISSN:1873-2518 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    Noroviruses (NoVs) are one of the major causes of acute viral gastroenteritis in humans. Virus-like particles (VLPs) without genomes that mimic the capsid structure of viruses are promising vaccine candidates for the prevention of NoVs infection. To produce large amounts of recombinant protein, including VLPs, the silkworm-expression vector system (silkworm-BEVS) is an efficient and powerful tool. In this study, we constructed a recombinant baculovirus that expresses VP1 protein, the major structural protein of NoV GII.4. Expression analysis showed that the baculovirus-infected silkworm pupae expressed NoV VP1 protein more efficiently than silkworm larval fat bodies. We obtained about 4.9 mg of purified NoV VP1 protein from only five silkworm pupae. The purified VP1 protein was confirmed by dynamic light scattering and electron microscopy to form VLPs of approximately 40 nm in diameter. Antisera from mice immunized with the antigen blocked NoV VLPs binding to histo-blood group antigens of pig gastric mucin and also blocked NoV infection in intestinal epithelial cells derived from human induced pluripotent stem (iPS) cells. Our findings demonstrated that NoV VLP eliciting protective antibodies could be obtained in milligram quantities from a few silkworm pupae using the silkworm-BEVS.

    DOI： 10.1016/j.vaccine.2022.12.015

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  • Characterization of a Novel Heterochromatin Protein 1 Homolog “HP1c” in the Silkworm, Bombyx mori Reviewed

    Masato Hino, Tsuneyuki Tatsuke, Akihiro Morio, Hiroaki Mon, Jae Man Lee, Akitsu Masuda, Kohei Kakino, Yoshino Tonooka, Takahiro Kusakabe

    Insects   13 ( 7 )   631 - 631   2022.7   （ ISSN:2075-4450 eISSN:2075-4450 ）

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    Language：Others   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Heterochromatin protein 1 plays an important role in chromatin structure and gene expression regulation. Three HP1 genes have been found in Homo sapiens, and five HP1 genes have been reported in Drosophila melanogaster. On the other hand, in Bombyx mori, only two HP1 genes, BmHP1a and BmHP1b, were reported. In this research, we have reported the molecular and functional characterization of a novel Bombyx mori HP1 gene (BmHP1c), which had stronger transcriptional repression activity than BmHP1a. BmHP1a and BmHP1b is reported to form homo- and heterodimers, but in co-immunoprecipitation experiments, no homo- or hetero-dimer formation of BmHP1c with the other silkworm HP1s is detected. The intracellular localization of BmHP1c is not only in the nucleus but also in the cytoplasm like mammalian HP1γ. In contrast to human HP1a and b, all three BmHP1s were localized preferentially in the regions poorly stained with DAPI. Interestingly, the double knockdown of BmHP1a and b, but not BmHP1c with a or b, arrested the cell cycle at the G2/M phase. These results suggest that BmHP1c is not essential for cell progression and plays a different role than BmHP1a and BmHP1b.

    DOI： 10.3390/insects13070631

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  • Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development Reviewed International journal

    Miyu Tanaka, Tsuguru Fujii, Hiroaki Mon, Jae Man Lee, Kohei Kakino, Hisayoshi Fukumori, Takeru Ebihara, Takumi Nagasato, Masato Hino, Yoshino Tonooka, Takato Moriyama, Ryosuke Fujita, Yutaka Banno, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   143   103737 - 103737   2022.4   （ ISSN:0965-1748 eISSN:1879-0240 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    The ovary is an important organ in reproduction. In insects, especially lepidopteran insects, the oocytes and reproductive organs develop rapidly during the pupal stage. Despite their drastic morphological changes, the molecular mechanisms of ovary development are not fully understood. In this study, it is found that forkhead box transcription factor L2, member 1 (FoxL21), which is known to be involved in ovarian differentiation and maintenance in vertebrates, is required for the development of the ovary in the silkworm, Bombyx mori. FoxL21 was expressed in the ovary and ovariole during the larval and pupal stage, respectively. In silkworms in which FoxL21 was knocked out by genome editing, multiple ovarian dysfunctions, such as, abnormal egg formation, thinning of the ovariole sheaths, and defective connection of the oviductus geminus with the ovariole were observed. Finally, ovarian transplantation experiments using the knockout silkworms revealed that FoxL21 functions in the ovariole, but not in the oviductus geminus.

    DOI： 10.1016/j.ibmb.2022.103737

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  • Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants Reviewed International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Hiroaki Mon, Keita Sato, Kosuke Oyama, Yasuteru Sakurai, Jiro Yasuda, Daisuke Takahashi, Tadashi Ueda, Yuri Kato, Motohiro Nishida, Noriko Karasaki, Kohei Kakino, Takeru Ebihara, Takumi Nagasato, Masato Hino, Ayaka Nakashima, Kengo Suzuki, Yoshino Tonooka, Miyu Tanaka, Takato Moriyama, Hirokazu Nakatake, Ryosuke Fujita, Takahiro Kusakabe

    Frontiers in Immunology   12   803647 - 803647   2022.1   （ ISSN:1664-3224 eISSN:1664-3224 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causing a spread of coronavirus disease 2019 (COVID-19) globally. In order to end the COVID-19 pandemic, an effective vaccine against SARS-CoV-2 must be produced at low cost and disseminated worldwide. The spike (S) protein of coronaviruses plays a pivotal role in the infection to host cells. Therefore, targeting the S protein is one of the most rational approaches in developing vaccines and therapeutic agents. In this study, we optimized the expression of secreted trimerized S protein of SARS-CoV-2 using a silkworm-baculovirus expression vector system and evaluated its immunogenicity in mice. The results showed that the S protein forming the trimeric structure was the most stable when the chicken cartilage matrix protein was used as the trimeric motif and could be purified in large amounts from the serum of silkworm larvae. The purified S protein efficiently induced antigen-specific antibodies in mouse serum without adjuvant, but its ability to induce neutralizing antibodies was low. After examining several adjuvants, the use of Alum adjuvant was the most effective in inducing strong neutralizing antibody induction. We also examined the adjuvant effect of paramylon from <italic>Euglena gracilis</italic> when administered with the S protein. Our results highlight the effectiveness and suitable construct design of the S protein produced in silkworms for the subunit vaccine development against SARS-CoV-2.

    DOI： 10.3389/fimmu.2021.803647

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  • Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice Reviewed

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Takeru Ebihara, Kohei Kakino, Masato Hino, Ryosuke Fujita, Hiroaki Mon, Takahiro Kusakabe

    Veterinary Research   52 ( 1 )   2021.12

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    DOI： 10.1186/s13567-021-00971-5

  • Non-molting dwarf (nm-d) as a mutant of Bombyx mori with a defect in purine synthesis Reviewed

    Tsuguru Fujii, Kohei Kakino, Hisayoshi Fukumori, Masato Hino, Jae Man Lee, Takahiro Kusakabe, Yutaka Banno

    Insect Biochemistry and Molecular Biology   138   103636 - 103636   2021.11

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    DOI： 10.1016/j.ibmb.2021.103636

  • Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System Reviewed

    Takeru Ebihara, Akitsu Masuda, Daisuke Takahashi, Masato Hino, Hiroaki Mon, Kohei Kakino, Tsuguru Fujii, Ryosuke Fujita, Tadashi Ueda, Jae Man Lee, Takahiro Kusakabe

    Molecular Biotechnology   2021.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-021-00373-0

  • Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system Reviewed

    Ryosuke Fujita, Masato Hino, Takeru Ebihara, Takumi Nagasato, Akitsu Masuda, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Kohei Kakino, Ryo Nagai, Miyu Tanaka, Yoshino Tonooka, Takato Moriyama, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   529 ( 2 )   257 - 262   2020.8

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    DOI： 10.1016/j.bbrc.2020.06.020

  • Efficient production of recombinant T7 endonuclease I using silkworm-baculovirus expression vector system Reviewed

    Kohei Kakino, Akitsu Masuda, Masato Hino, Takeru Ebihara, Jian Xu, Hiroaki Mon, Ryosuke Fujita, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   23 ( 3 )   694 - 700   2020.8

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  • Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system Reviewed

    Masahiko Kobayashi, Jian Xu, Kohei Kakino, Akitsu Masuda, Masato Hino, Naoki Fujimoto, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Hiroshi Iida, Masateru Takahashi, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   23 ( 1 )   268 - 273   2020.4

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    DOI： 10.1016/j.aspen.2019.12.014

  • Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells Reviewed

    Masato Hino, Noriko Karasaki, Tsuneyuki Tatsuke, Daisuke Morokuma, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   89 ( 1 )   9 - 16   2020.1

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    DOI： 10.11416/jibs.89.1_009

  • Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System Reviewed

    61 ( 8 )   622 - 630   2019.8

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    Type IB DNA topoisomerases are enzymes to change the topological state of DNA molecules and are essential in studying replication, transcription, and recombination of nucleic acids in vitro. DNA topoisomerase IB from Vaccinia virus (vTopIB) is a 32 kDa, type I eukaryotic topoisomerase, which relaxed positively and negatively supercoiled DNAs without Mg2+ and ATP. Although vTopIB has been effectively produced in E. coli expression system, no studies remain available to explore an alternative platform to express recombinant vTopIB (rvTopIB) in a higher eukaryote, where the one can expect post-translational modifications that affect the activity of rvTopIB. Here in this study, rvTopIB with N-terminal tags was constructed and expressed in a silkworm–baculovirus expression vector system (silkworm–BEVS). We developed a simple two consecutive chromatography purification to obtain highly pure rvTopIB. The final yield of rvTopIB obtained from a baculovirus-infected silkworm larva was 83.25 μg. We also evaluated the activity and function of rvTopIB by the DNA relaxation activity assays using a negatively supercoiled pUC19 plasmid DNA as a substrate. With carefully assessing optimized conditions for the reaction buffer, we found that divalent ions, Mg2+, Mn2+, Ca2+, as well as ATP stimulate the DNA relaxation activity by rvTopIB. The functional and active form of rvTopIB, together with the yields of the protein we obtained, suggests that silkworm–BEVS would be a potential alternative platform to produce eukaryotic topoisomerases on an industrial scale.

    DOI： 10.1007/s12033-019-00184-4

  • Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system Reviewed

    22 ( 2 )   404 - 408   2019.6

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    The O-glycosidase, endo-α-N-acetylgalactosaminidase from Enterococcus faecalis (endoEF) catalyzes the cleavage of core 1 and core 3 type O-linked disaccharides between GalNAc and serine or threonine residues from glycoproteins. The endoEF has broad substrate specificity and thus is extensively utilized for the structural and functional analysis of the O-linked glycans. In this study, we expressed and purified the recombinant endoEF (rEndoEF) by using the silkworm-baculovirus expression vector system (Silkworm-BEVS) and confirmed the deglycosylation activity of rEndoEF targeting reporter glycoproteins, which was equivalent to the commercial O-glycosidase. Thus, our study provides important clues to produce highly active rEndoEF O-glycosidases employing silkworm-BEVS as an alternative.

    DOI： 10.1016/j.aspen.2019.01.009

  • Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system Reviewed

    Takumi Yano, Jae Man Lee, Jian Xu, Yoshiki Morifuji, Akitsu Masuda, Masato Hino, Daisuke Morokuma, Ryosuke Fujita, Masateru Takahashi, Takahiro Kusakabe, Hiroaki Mon

    Journal of Asia-Pacific Entomology   22 ( 2 )   453 - 457   2019.6

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    DOI： 10.1016/j.aspen.2019.02.008

  • A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells Reviewed

    Jian Xu, Akihiro Morio, Daisuke Morokuma, Yudai Nagata, Masato Hino, Akitsu Masuda, Zhiqing Li, Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee

    Applied Microbiology and Biotechnology   102 ( 20 )   8783 - 8797   2018.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-018-9309-6

  • Production of a biologically active human basic fibroblast growth factor using silkworm-baculovirus expression vector system Reviewed

    Akitsu Masuda, Jian Xu, Kosuke Minamihata, Genki Kagawa, Yusei Hamada, Yoshiki Morifuji, Takumi Yano, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   21 ( 2 )   716 - 720   2018.6

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    DOI： 10.1016/j.aspen.2018.05.002

  • A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology Reviewed

    Morokuma D, Hino M, Tsuchioka M, Masuda A, Mon H, Fujiyama K, Kajiura H, Kusakabe T, Lee JM

    36 ( 1 )   15 - 24   2018.3

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    A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology

  • Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm–Baculovirus Expression System Reviewed

    2018.1

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    Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.

    DOI： 10.1007/s12033-018-0127-y

  • Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae Reviewed

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Tetsuo Sato, Shizuka Hayashi, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Takahiro Kusakabe

    Journal of General Virology   99 ( 7 )   917 - 926   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/jgv.0.001087

  • Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase Reviewed

    Akitsu Masuda, Kosuke Minamihata, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Jae Man Lee

    Journal of Insect Biotechnology and Sericology   87 ( 2 )   53 - 60   2018.1

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    DOI： 10.11416/jibs.87.2_053

  • Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   493 ( 2 )   971 - 978   2017.11

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    DOI： 10.1016/j.bbrc.2017.09.107

  • Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori Reviewed

    Ming Ming Ji, Jae Man Lee, Hiroaki Mon, Kazuhiro Iiyama, Tsuneyuki Tatsuke, Daisuke Morokuma, Masato Hino, Mami Yamashita, Kazuma Hirata, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   89   86 - 96   2017.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2017.08.006

  • Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   490 ( 2 )   134 - 140   2017.8

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    DOI： 10.1016/j.bbrc.2017.06.008

  • Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System Reviewed

    Mami Yamashita, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Masato Hino, Hiroaki Mon, Masateru Takahashi, Samir M. Hamdan, Kosuke Sakashita, Kazuhiro Iiyama, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Molecular Biotechnology   59 ( 6 )   221 - 233   2017.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-017-0008-9

  • Expression and Characterization of Human β-1, 4-Galactosyltransferase 1 (β4GalT1) Using Silkworm–Baculovirus Expression System Reviewed

    59 ( 4-5 )   151 - 158   2017.5

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    Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches have been reported to alter N-linked glycan structures of glycoproteins derived from insects into terminally sialylated complex-type N-glycans. In the light of those studies, we also proposed in vitro maturation of N-glycan with mass-produced and purified glycosyltransferases by silkworm–BEVS. β-1,4-Galactosyltransferase 1 (β4GalT1) is known as one of type II transmembrane enzymes that transfer galactose in a β-1, 4 linkage to accepter sugars, and a key enzyme for further sialylation of N-glycans. In this study, we developed a large-scale production of recombinant human β4GalT1 (rhβ4GalT1) with N- or C-terminal tags in silkworm–BEVS. We demonstrated that rhβ4GalT1 is N-glycosylated and without mucin-type glycosylation. Interestingly, we found that purified rhβ4GalT1 from silkworm serum presented higher galactosyltransferase activity than that expressed from cultured mammalian cells. We also validated the UDP-galactose transferase activity of produced rhβ4GalT1 proteins by using protein subtracts from silkworm silk gland. Taken together, rhβ4GalT1 from silkworms can become a valuable tool for producing high-quality recombinant glycoproteins with mammalian-like N-glycans.

    DOI： 10.1007/s12033-017-0003-1

  • Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus Reviewed

    Jianping Chen, Jian Xu, Masato Hino, Mami Yamashita, Kazuma Hirata, Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   19 ( 3 )   753 - 760   2016.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2016.07.007

  • High-level expression and purification of biologically active human IL-2 using silkworm-baculovirus expression vector system Reviewed

    Masato Hino, Takuji Kawanami, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Mami Yamashita, Noriko Karasaki, Tuneyuki Tatsuke, Hiroaki Mon, Kazuhiro Iiyama, Noriho Kamiya, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   19 ( 2 )   313 - 317   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2016.03.014

  • Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori Reviewed

    Masato Hino, Daisuke Morokuma, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   85 ( 2 )   21 - 29   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.85.2_021

  • Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system Reviewed

    Daisuke Morokuma, Jian Xu, Hiroaki Mon, Kazuma Hirata, Masato Hino, Shoko Kuboe, Mami Yamashita, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   18 ( 2 )   303 - 309   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2015.03.006

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Presentations

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MISC

  • カイコ精巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析

    柿野耕平, 門宏明, 佐藤昌直, 藤井告, 日野真人, 李在萬, 藤田龍介, 日下部宜宏

    蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集   92nd   2022

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  • 特集「ポストNGSの昆虫科学を考える」 ポストNGS時代のカイコ研究

    日野 真人

    蚕糸・昆虫バイオテック   2021.6

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    Language：Japanese  

    DOI： 10.11416/konchubiotec.90.1_035

Professional Memberships

  • 日本衛生動物学会南日本支部

    2021.9 - Present

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  • The Japan Society of Medical Entomology and Zoology

    2021.1 - Present

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  • The Japanese Society of Sericultural Science, Kyushu Branch

  • The Japanese Society of Sericultural Science

  • The Molecular Biology Society of Japan

  • The Japan Society of Medical Entomology and Zoology

  • 日本衛生動物学会南日本支部

  • The Japanese Society of Sericultural Science, Kyushu Branch

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  • The Japanese Society of Sericultural Science

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  • The Molecular Biology Society of Japan

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Committee Memberships

  • 第 75 回 日本寄生虫学会 南日本支部大会 第 72 回 日本衛生動物学会 南日本支部大会   事務局長  

    2023.10   

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    Committee type：Other

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  • Steering committee member   Domestic

    2023.3 - 2025.2   

  • 日本蚕糸学会 若手の会   運営委員  

    2023.3 - 2025.2   

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    Committee type：Academic society

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  • 令和4年度 蚕糸・昆虫機能利用学術講演会 ― 日本蚕糸学会第 92 回大会 ―   事務局  

    2022.3   

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    Committee type：Academic society

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  • Steering committee member   Domestic

    2021.4 - 2022.3   

  • 日本蚕糸学会九州支部   支部委員、広報幹事、講演集編集委員   Domestic

    2021.4 - 2022.3   

  • 日本蚕糸学会九州支部   支部委員、広報幹事、講演集編集委員  

    2021.4 - 2022.3   

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    Committee type：Academic society

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Academic Activities

  • 事務局長

    第75回日本寄生虫学会・第72回日本衛生動物学会 南日本支部合同大会  （ 九州大学 ） 2023.10

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    Type：Competition, symposium, etc. 

  • 運営・企画

    令和 5 年度 日本蚕糸学会若手の会主催 「蚕糸学生交流講演会」  （ オンライン ） 2023.9

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    Type：Competition, symposium, etc. 

  • 学術論文等の審査

    Role(s)： Peer review

    2023.8

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：1

  • 展示企画・運営

    ふくおか大昆虫展 inももち  （ TNC放送会館1Fエントランス ） 2023.7 - 2023.8

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    Type：Competition, symposium, etc. 

  • 実行委員会

    第 6 回 九州大学昆虫科学・新産業創生研究センター&第 79 回昆虫病理研究会 合同シンポジウム  （ 九州大学（オンラインハイブリッド） ） 2022.11

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    Type：Competition, symposium, etc. 

  • 事務局

    令和４年度　蚕糸・昆虫機能利用学術講演会　日本蚕糸学会第９２回大会  （ オンライン ） 2022.3

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    Type：Competition, symposium, etc. 

  • 実行委員会

    2021 年度 九州大学大学院農学研究院附属 昆虫科学・新産業創生研究センターシンポジウム  （ 九州大学 ） 2021.8

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    Type：Competition, symposium, etc. 

  • 実行委員

    2020年度 九州大学大学院農学研究院附属 昆虫科学・新産業創生研究センターシンポジウム  （ 九州大学（オンラインハイブリッド） ） 2021.3

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    Type：Competition, symposium, etc. 

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Research Projects

  • カイコ断片染色体を利用した人工染色体作製と断片染色体脱落率決定因子の同定

    Grant number：22K14900  2022 - 2024

    日本学術振興会  科学研究費助成事業  若手研究

    日野 真人

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    Authorship：Principal investigator  Grant type：Scientific research funding

    カイコは重要な実験昆虫、産業昆虫の１つであり、有用組換えタンパク質生産などに利用されている。その中で、大量の外来遺伝子導入が必要とされているが、大量の遺伝子導入手法は確立されておらず、システムの開発が求められている。そこで、本研究ではカイコまだら系統の持つサイズの小さな断片染色体を利用し大量遺伝子導入に使用可能なカイコ人工染色体作製を試みる。また、複数のまだら系統が存在し、系統によっては断片染色体が細胞から脱落しやすい。そこで、本研究では複数の断片染色体を比較解析し、カイコ細胞において染色体が維持されるのに必要な領域を決定する。

    CiNii Research

  • カイコHP1のヘテロクロマチン形成における役割の解明

    2022

    令和４年度大学院農学研究院若手教員支援事業(チャレンジ研究支援（Ⅰ型）)

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

  • 北海道石狩マクンベツ湿原に生息する蚊より分離されたHubei mosquito virus 4 (Ishikari strain)の解析

    2020

    令和２年度農学研究院若手教員支援事業 チャレンジ研究支援(I型)

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

  • カイコの複製分子基盤の解明と人工染色体構築

    2017 - 2019

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 複製誘導因子を利用したカイコ人工染色体の構築

    2016

    九州大学QRECアカデミック・チャレンジ研究資金補助

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

Class subject

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 農業生物科学プロジェクト演習

    2023.10 - 2023.12   Fall quarter

  • 実験で学ぶ自然科学

    2023.6 - 2023.8   Summer quarter

  • 修士論文研究Ⅱ

    2023.4 - 2024.3   Full year

  • 農業生物科学ティーチング演習

    2023.4 - 2024.3   Full year

  • 演示技法Ⅰ

    2023.4 - 2024.3   Full year

  • 演示技法Ⅱ

    2023.4 - 2024.3   Full year

  • 特別演習Ⅱ

    2023.4 - 2023.9   First semester

  • 農業生物科学プロジェクト演習

    2023.4 - 2023.6   Spring quarter

  • 農業生物科学演習第二

    2022.10 - 2023.3   Second semester

  • 農業生物科学演習第一

    2022.10 - 2023.3   Second semester

  • 農業生物科学プロジェクト演習

    2022.10 - 2022.12   Fall quarter

  • 修士論文研究Ⅰ

    2022.4 - 2023.3   Full year

  • 農業生物科学ティーチング演習

    2022.4 - 2023.3   Full year

  • 演示技法Ⅰ

    2022.4 - 2023.3   Full year

  • 演示技法Ⅱ

    2022.4 - 2023.3   Full year

  • 特別演習Ⅰ

    2022.4 - 2022.9   First semester

  • 農業生物科学プロジェクト演習

    2022.4 - 2022.6   Spring quarter

  • 農業生物科学演習第二

    2021.10 - 2022.3   Second semester

  • 農業生物科学演習第一

    2021.10 - 2022.3   Second semester

  • 農業生物科学プロジェクト演習

    2021.10 - 2021.12   Fall quarter

  • 自然科学総合実験

    2021.6 - 2021.8   Summer quarter

  • 演示技法Ⅱ

    2021.4 - 2022.3   Full year

  • 農業生物科学ティーチング演習

    2021.4 - 2022.3   Full year

  • 演示技法Ⅰ

    2021.4 - 2022.3   Full year

  • 農業生物科学プロジェクト演習

    2021.4 - 2021.6   Spring quarter

  • 農業生物科学演習第二

    2020.12 - 2021.2   Winter quarter

  • 農業生物科学演習第一

    2020.12 - 2021.2   Winter quarter

  • 農業生物科学演習第二

    2020.10 - 2020.12   Fall quarter

  • 農業生物科学プロジェクト演習

    2020.10 - 2020.12   Fall quarter

  • 農業生物科学演習第一

    2020.10 - 2020.12   Fall quarter

  • 演示技法Ⅱ

    2020.4 - 2021.3   Full year

  • 農業生物科学ティーチング演習

    2020.4 - 2021.3   Full year

  • 演示技法Ⅰ

    2020.4 - 2021.3   Full year

  • 農業生物科学プロジェクト演習

    2020.4 - 2020.6   Spring quarter

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FD Participation

  • 2024.4   Role：Participation   Title：【全学FD】自殺防止メンタルヘルス研修会

    Organizer：University-wide

  • 2024.3   Role：Participation   Title：有体物管理センターの業務および成果有体物収入の配分率の変更について

    Organizer：University-wide

  • 2023.11   Role：Participation   Title：農学研究院FD：遺伝子組換え実験の安全管理について

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2022.12   Role：Participation   Title：電子教材著作権講習会

    Organizer：University-wide

  • 2022.3   Role：Participation   Title：農学研究院FD：リポジトリ登録を対象とした大学改革推進経費の新指標案について

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.12   Role：Participation   Title：電子教材著作権講習会

    Organizer：University-wide

  • 2021.9   Role：Participation   Title：JST 次世代研究者挑戦的研究プログラム 説明会

    Organizer：University-wide

  • 2021.7   Role：Participation   Title：農学研究院FD「科研費を獲りにいこう！　科研費獲得の技術と工夫」

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.4   Role：Participation   Title：オンライン授業実施の”いろは”

    Organizer：University-wide

  • 2021.4   Role：Participation   Title：令和３年度 第１回全学ＦＤ（新任教員の研修）

    Organizer：University-wide

  • 2021.4   Role：Participation   Title：2021年度新任教員教育セミナー

    Organizer：University-wide

  • 2020.12   Role：Participation   Title：大学の研究評価の現状と農学研究院の「部局独自の評価基準」案における業績分析

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.9   Role：Participation   Title：科研費を獲りにいこう！　勝ち抜く気合と技術

    Organizer：[Undergraduate school/graduate school/graduate faculty]

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Other educational activity and Special note

  • 2022  Class Teacher 

  • 2021  Class Teacher 

Outline of Social Contribution and International Cooperation activities

  • Participated in a bilateral exchange program with Shanghai Jiao Tong University

Social Activities

  • 特別講座「カイコが支えるヒトの未来」 病気予防と昆虫食～リアルなカイコや食品をみてみよう

    Role(s)： Lecturer

    九州環境管理協会  2024.2

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    Type：Seminar, workshop

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  • 特別講座「カイコが支えるヒトの未来」 病気予防と昆虫食～リアルなカイコや食品をみてみよう

    九州環境管理協会  2024.2

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    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

  • ふくおか大昆虫展inももち ～世界の昆虫と九州大学の研究～

    Role(s)： Planner,　Organizing member

    九州大学総合研究博物館、九州大学昆虫科学・新産業創生研究センター、TNCプロジェクト  2023.7 - 2023.8

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    Type：Other

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  • ふくおか大昆虫展inももち　～世界の昆虫と九州大学の研究～

    九州大学総合研究博物館、九州大学昆虫科学・新産業創生研究センター、TNCプロジェクト  TNC放送会館1F特設会場  2023.7

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    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Other

    九州大学は日本でもっとも昆虫研究がさかんです。新種を発見したり図鑑を作ったり進化の道筋を研究するような基礎的な学問から、昆虫をもとにワクチンなどの医薬品を作ったり、翅のたたみ方を最先端の応用分野に活かすなどたくさんの研究者が昆虫を通じてさまざまな研究を進めています。また九州大学は日本でもっとも多くの昆虫標本を所蔵しその数は400万点を超えます。この展示では大学で行われている研究を紹介するとともにたくさんの標本の中からよりすぐりの美麗な世界の昆虫約4000点を展示しました。また医療や食の分野でも活躍する昆虫研究も紹介し、子供たちが楽しく学べる内容で構成しました。