Updated on 2024/09/27

Information

 

写真a

 
MASUDA YOSHIMITSU
 
Organization
Faculty of Agriculture Department of Bioscience and Biotechnology Assistant Professor
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928024794
Profile
We are mainly aiming to improve food safety and guarantee high-quality products by studying on food-born pathogens such as Salmonella, Campylobacter, and Listeria. My study is aiming to evaluate the influence of toxin-antitoxin systems of those bacteria on their stress tolerance and clarify these mechanisms. Especially, my study is focusing on the influence of toxin-antitoxin system on 1) heat stress conditions and 2) injured cells caused by heat treatment. Also, we are working on establishment of novel bacteriophage treatment to control food-born pathogens.
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External link

Degree

  • PhD (Agricultural Science)

Research History

  • 平成24年4月〜11月                  学術研究員、九州大学大学院農学研究院生命機能科学部門 分子微生物学・バイオマス資源科学講座 微生物工学研究室 平成24年12月〜平成26年1月   ポスドク研究員、Molecular Genetics、 GBB、 Groningen, オランダ王国 平成26年3月〜平成28年2月      JSPS海外特別研究員、Molecular Genetics、GBB、University of Groningen、オランダ王国

Research Interests・Research Keywords

  • Research theme:Genetical modification of bacteriophage by genome editing and LLB-phage application

    Keyword:bacteriophage, genome editing, CRISPR-Cas, bacteriocin, leaderless bacteriocin

    Research period: 2022.4 - 2025.3

  • Research theme:Study on stress tolerance of pathogenic bacterial persister cells

    Keyword:pathogenic bacteria, stress tolerance, Persister cells

    Research period: 2020.4 - 2023.3

  • Research theme:Establishment of novel food-born pathogen control method by leaderless bacteriocin producing phage

    Keyword:food-born pathogen, phage, leaderless bacteriocin

    Research period: 2018.4 - 2022.3

  • Research theme:Role of toxin-antitoxin system in bacterial heat tolerance

    Keyword:Food-born pathogen, heat tolerance, toxin-antitoxin, persiter

    Research period: 2017.4 - 2020.3

  • Research theme:Biosynthetic analyses on circular and leaderless bacteriocins produced by lactic acid bacteria

    Keyword:bacteriocin, lactic acid bacteria, leaderless bacteriocin, circular bacteriocin

    Research period: 2012.4 - 2012.11

  • Research theme:Study on novel bacteriocins from lactic acid bacteria, circular and leaderless bacteriocins

    Keyword:bacteriocin, lactic acid bacteria, leaderless bacteriocin, circular bacteriocin, multiple-bacteriocin producing bacteria

    Research period: 2009.4 - 2012.3

  • Research theme:Study on structural and characteristic analyses of novel bacteriocins produced by Weissella hellenica QU 13

    Keyword:bacteriocin, lactic acid bacteria, antimicrobial peptide

    Research period: 2007.4 - 2009.3

Awards

  • 令和4年度日本食品科学工学会奨励賞

    2022.8   日本食品科学工学会   「細菌の熱耐性および熱損傷の機構に関する研究」

  • 日本農芸化学会西日本支部奨励賞

    2021.3   公益社団法人日本農芸化学会   TAシステム制御下のPersister集団とインドール産生が細菌の耐熱性に及ぼす影響

Papers

  • Application of endolysin LysSTG2 as a potential biocontrol agent against planktonic and biofilm cells of Pseudomonas on various food and food contact surfaces Reviewed International journal

    Yu Zhang, Hung-Hsin Huang, Hoang Minh Duc, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Food Control   131   2022.1

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    DOI: https://doi.org/10.1016/j.foodcont.2021.108460

  • Construction of Leaderless-Bacteriocin-Producing Bacteriophage Targeting E. coli and Neighboring Gram-Positive Pathogens Reviewed International journal

    9 ( 1 )   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/Spectrum.00141-21

    Repository Public URL: https://hdl.handle.net/2324/7236452

  • Role of Toxin-Antitoxin-Regulated Persister Population and Indole in Bacterial Heat Tolerance Reviewed International journal

    Yoshimitsu Masuda, Erika Sakamoto, Ken-ichi Honjoh, Takahisa Miyamoto

    Applied and Environmental Microbiology   86 ( 16 )   2020.8

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    DOI: 10.1128/AEM.00935-20

    Repository Public URL: https://hdl.handle.net/2324/7236460

  • Antibacterial effect and mechanism of theaflavin against <i>Listeria monocytogenes</i> and its application on apple skins

    Lin, YZ; Shen, CK; Zhao, JX; Wang, C; Obara, M; Maung, AT; Morita, M; Abdelaziz, MNS; Masuda, Y; Honjoh, KI; Miyamoto, T

    JOURNAL OF FOOD SCIENCE   2024.9   ISSN:0022-1147 eISSN:1750-3841

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    Theaflavin 3,3′-digallate (TF3), a major polyphenolic component of black tea, exhibits antibacterial effects against many foodborne pathogens. However, the antibacterial mechanisms of TF3 against Listeria monocytogenes remain unclear. In this study, we investigated the effects of TF3 on viability, biofilm, and membrane function of L. monocytogenes by the conventional plating method, crystal violet staining, and microscopy using fluorescent dyes JC-1 and Laurdan, respectively. It was found that TF3 showed excellent antibacterial activity against L. monocytogenes with the minimum inhibitory concentration of 62.5 mg/L. The viable count determined on TSA decreased by 3 log after the treatment for 2 h with TF3 at 62.5 mg/L. The viable count determined on TSA containing 4% NaCl decreased by more than 4 log after the treatment for 30 min with TF3 at the same concentration, suggesting that TF3 gave damage on the cells, enhancing the antibacterial action of 4% NaCl, but the damage was recoverable in the absence of 4% NaCl. To explore the antibacterial mechanisms of TF3, the effects of TF3 on membrane potential and membrane fluidity were investigated. TF3 reduced both membrane potential and fluidity of L. monocytogenes at 62.5 mg/L, suggesting that TF3 damaged the structural integrity of the cell membrane. TF3 reduced biofilm mass of mature biofilm of L. monocytogenes. Moreover, THEAFLAVIN TF40, a commercially available Camellia sinensis leaf extract containing TF3, reduced viable count of L. monocytogenes by 2 log on apple skin. These results suggest the potential of theaflavins as a natural anti-Listeria disinfectant.

    DOI: 10.1111/1750-3841.17321

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  • Applications of bacteriophage in combination with nisin for controlling multidrug-resistant Bacillus cereus in broth and various food matrices

    Abdelaziz, MNS; Maung, AT; El-Telbany, M; Lwin, SZC; Mohammadi, TN; Zayda, M; Wang, C; Damaso, CH; Lin, YZ; Masuda, Y; Honjoh, KI; Miyamoto, T

    FOOD RESEARCH INTERNATIONAL   191   114685   2024.9   ISSN:0963-9969 eISSN:1873-7145

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    This study focused on the isolation and characterization of bacteriophages with specific activity against toxin-producing and multidrug-resistant strains of Bacillus cereus sensu stricto (B. cereus s. s.). Ten different samples yielded six bacteriophages by utilizing the double-layer agar technique. The most promising phage, vB_BceS-M2, was selected based on its broad host range and robust lytic activity against various B. cereus s. s. strains. The phage vB_BceS-M2 had a circular double-stranded DNA genome of 56,482 bp. This phage exhibited stability over a wide range of temperatures and pH values, which is crucial for its potential application in food matrices. The combined effect of phage vB_BceS-M2 and nisin, a widely used antimicrobial peptide, was investigated to enhance antimicrobial efficacy against B. cereus in food. The results suggested that nisin showed synergy and combined effect with the phage, potentially overcoming the growth of phage-resistant bacteria in the broth. Furthermore, practical applications were conducted in various liquid and solid food matrices, including whole and skimmed milk, boiled rice, cheese, and frozen meatballs, both at 4 and 25 °C. Phage vB_BceS-M2, either alone or in combination with nisin, reduced the growth rate of B. cereus in foods other than whole milk. The combination of bacteriophage and nisin showed promise for the development of effective antimicrobial interventions to counteract toxigenic and antibiotic-resistant B. cereus in food.

    DOI: 10.1016/j.foodres.2024.114685

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  • Single and combined application of bacteriophage and cinnamon oils against pathogenic Listeria monocytogenes in milk and smoked salmon

    Maung, AT; Abdelaziz, MNS; Mohammadi, TN; Lwin, SZC; El-Telbany, M; Zhao, JX; Wang, C; Lin, YZ; Shen, CK; Zayda, M; Masuda, Y; Honjoh, KI; Miyamoto, T

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   421   110797   2024.8   ISSN:0168-1605 eISSN:1879-3460

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    Language:English   Publisher:International Journal of Food Microbiology  

    Nowadays, the discovery of alternative natural antimicrobial substances such as bacteriophages, essential oils, and other physical and chemical agents is developing in the food industry. In this study, nine bacteriophages were isolated from various parts of raw chickens and exhibited lytic activities against L. monocytogenes and various Listeria spp. The characterization of phage vB_LmoS-PLM9 was stable at 4 to 50 °C and pH range from 4 to 10. Phage vB_LmoS-PLM9 had a circular, double-stranded genomic DNA with 38,345 bp having endolysin but no antibiotic resistance or virulence genes. Among the eight essential oils tested at 10 %, cinnamon bark, and cassia oils showed the strongest antilisterial activities. The combined use of phage vB_LmoS-PLM9 and cinnamon oils indicated higher efficiency than single treatments. The combination of phage (MOI of 10) and both cinnamon oils (0.03 %) reduced the viable counts of L. monocytogenes and inhibited the regrowth of resistant cell populations in broth at 30 °C. Furthermore, treatment with the combination of phage (MOI of 100) and cinnamon oil (0.125 %) was effective in milk, especially at 4 °C by reducing the viable count to less than lower limit of detection. These results suggest combining phage and cinnamon oil is a potential approach for controlling L. monocytogenes in milk.

    DOI: 10.1016/j.ijfoodmicro.2024.110797

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  • Effects of EGCg and theaflavins on emetic toxin-producing <i>Bacillus cereus </i>in vitro

    Yuan, L; Matsuda, A; Abdelaziz, MNS; Masuda, Y; Honjoh, K; Miyamoto, T

    EUROPEAN FOOD RESEARCH AND TECHNOLOGY   2024.8   ISSN:1438-2377 eISSN:1438-2385

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    Publisher:European Food Research and Technology  

    Bacillus cereus is a spore-forming foodborne pathogen that causes emetic and diarrheal food poisoning. An emetic strain of B. cereus produces spores and an emetic toxin called cereulide, which exhibits high heat resistance. In this study, we investigated the effects of epigallocatechin gallate (EGCg), theaflavin-3’-gallate (TF2b), and theaflavin-3,3’-gallate (TF3), polyphenolic components of tea extracts, on the survivability, spores, and toxin of B. cereus. Among them, EGCg at 62.5 μg/mL and 250 μg/mL showed bacteriostatic and bactericidal effects on B. cereus by damaging the cellular membrane, causing leakage of cellular nucleic acid related substances, and thus inhibiting the growth of B. cereus. However, no significant effects were shown on spore germination by EGCg, TF2b, or TF3 at a concentration of 250 μg/mL in this investigation. Their inhibitory effects were primarily confined to the outgrowth of daughter cells after spore germination. Furthermore, quantitative polymerase chain reaction (qPCR) showed that they suppressed the activity of cesA, a gene associated with cereulide production. At concentrations of 125 µg/mL for TF2b and 62.5 µg/mL for TF3, a discernible attenuation of cytotoxic effects induced by cereulide on HEp-2 cells was evident.

    DOI: 10.1007/s00217-024-04593-x

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  • Identification of Enterococcus spp. from food sources by matrix-assisted laser desorption ionization-time of flight mass spectrometry and characterization of virulence factors, antibiotic resistance, and biofilm formation

    Wang, C; El-Telbany, M; Lin, YZ; Zhao, JX; Maung, AT; Abdelaziz, MNS; Nakayama, M; Masuda, Y; Honjoh, KI; Miyamoto, T

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   420   110768   2024.8   ISSN:0168-1605 eISSN:1879-3460

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    Language:English   Publisher:International Journal of Food Microbiology  

    The continuous detection of multi-drug-resistant enterococci in food source environments has aroused widespread concern. In this study, 198 samples from chicken products, animal feces, raw milk, and vegetables were collected in Japan and Egypt to investigate the prevalence of enterococci and virulence characterization. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed for species identification and taxonomic analysis of the isolates. The results showed that the rates of most virulence genes (efaA, gelE, asa1, ace, and hyl) in the Japanese isolates were slightly higher than those in the Egyptian isolates. The rate of efaA was the highest (94.9 %) among seven virulence genes detected, but the cylA gene was not detected in all isolates, which was in accordance with γ-type hemolysis phenotype. In Enterococcus faecalis, the rate of kanamycin-resistant strains was the highest (84.75 %) among the antibiotics tested. Moreover, 78 % of E. faecalis strains exhibited multi-drug resistance. Four moderately vancomycin-resistant strains were found in Egyptian isolates, but none were found in Japanese isolates. MALDI-TOF MS analysis correctly identified 98.5 % (68/69) of the Enterococcus isolates. In the principal component analysis dendrogram, strains isolated from the same region with the same virulence characteristics and similar biofilm-forming abilities were characterized by clustered distribution in different clusters. This finding highlights the potential of MALDI-TOF MS for classifying E. faecalis strains from food sources.

    DOI: 10.1016/j.ijfoodmicro.2024.110768

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  • Genetic Characterization, Antibiotic Resistance, and Virulence Genes Profiling of <i>Bacillus cereus</i> Strains from Various Foods in Japan

    Abdelaziz, MNS; Zayda, MG; Maung, AT; El-Telbany, M; Mohammadi, TN; Lwin, SZC; Linn, KZ; Wang, C; Yuan, L; Masuda, Y; Honjoh, K; Miyamoto, T

    ANTIBIOTICS-BASEL   13 ( 8 )   2024.8   ISSN:2079-6382

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    Bacillus cereus sensu stricto is a foodborne pathogen that causes food poisoning. Their spore and biofilm-forming abilities persist in various environments and foods. This study investigated the prevalence, virulence, antibiotic resistance, and genetic diversity of B. cereus s. s. strains isolated from various food samples. Of 179 samples, 22.34% were positive for B. cereus s. s., with significantly high detection rates in milk products and raw chicken meat. Forty strains were isolated from positive samples. Matrix-assisted laser desorption ionization/time of flight mass spectrometry analysis revealed nine distinct clusters and multi-locus sequence typing revealed 34 sequence types including 23 novel sequences, demonstrating high genetic diversity among the isolates. PCR analysis revealed that all the strains contained at least one toxin gene, but none contained the cytK gene. Antibiotic resistance tests revealed that all isolates were classified as multidrug-resistant, with high resistance levels, particularly to β-lactam antibiotics and vancomycin, but were susceptible to gentamicin. All isolates showed variations in biofilm formation. This study highlights the significant public health risk due to B. cereus s. s. and underscores the need for stringent monitoring and control measures in food production to manage antimicrobial resistance and ensure food safety.

    DOI: 10.3390/antibiotics13080774

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  • haracterization and antibacterial activity of highly thermo- and pH-stable endolysin LysCPQ7 and its application as a biocontrol agent against Clostridium perfringens in milk and cheese Reviewed International journal

    @Tahir Noor Mohammadi, #Yunzhi Lin, #Aye Thida Maung, @Cunkuan Shen, @Junxin Zhao, #Mohamed El-Telbany, @Mahmoud Zayda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Food Control   156   2024.2

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    https://www.sciencedirect.com/science/article/pii/S0956713523005571

    DOI: https://doi.org/10.1016/j.foodcont.2023.110157

    Other Link: 著作権侵害(出版社等)により不許可

    Repository Public URL: 100044

  • Characterization and antibacterial activity of highly thermo- and pH-stable endolysin LysCPQ7 and its application as a biocontrol agent against <i>Clostridium perfringens</i> in milk and cheese

    Mohammadi, TN; Lin, YZ; Maung, AT; Shen, CK; Zhao, JX; El-Telbany, M; Zayda, M; Masuda, Y; Honjoh, KI; Miyamoto, T

    FOOD CONTROL   156   2024.2   ISSN:0956-7135 eISSN:1873-7129

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    Phage-encoded peptidoglycanases (phage endolysins) are hydrolyzing enzymes that break peptidoglycan bonds within infected bacterial cell walls at the end of the lytic cycle. They are promising antibacterial agents capable of controlling major foodborne pathogens. Here, we cloned, overexpressed, and purified the phage-encoded protein LysCPQ7, a putative endolysin from the Clostridium perfringens phage CPQ7. The predicted amino acid sequence indicated that LysCPQ7 exhibited amidase activity to digest the peptidoglycan bond between muramic acid and peptide in bacterial cell walls. LysCPQ7 was characterized as an alkalophilic and thermostable endolysin. It exhibited the highest lytic activity against C. perfringens cells at pH values ranging from 9.0 to 11.0, and was stable at temperatures from −20 to 60 °C. The lytic activity of LysCPQ7 was not significantly (p < 0.05) influenced in the absence/presence of 100, 200, and 300 mM of NaCl. The antibacterial test confirmed that LysCPQ7 reduced the viability of C. perfringens cells by 4.1 log after 6 h of incubation in broth. LysCPQ7 significantly (p < 0.05) decreased the viability of C. perfringens in artificially-contaminated milk and sliced cheese under refrigeration and room temperatures. Our results showed that LysCPQ7 has great potential as a newly emerging biocontrol agent against C. perfringens in dairy and fermented food products.

    DOI: 10.1016/j.foodcont.2023.110157

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  • Characterization of selected phages for biocontrol of food-spoilage pseudomonads

    Johno, D; Zhang, Y; Mohammadi, TN; Zhao, JX; Lin, YZ; Wang, C; Lu, Y; Abdelaziz, MNS; Maung, AT; Lin, CY; El-Telbany, M; Lwin, SZC; Damaso, CH; Masuda, Y; Honjoh, KI; Miyamoto, T

    INTERNATIONAL MICROBIOLOGY   27 ( 4 )   1333 - 1344   2024.1   ISSN:1139-6709 eISSN:1618-1905

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    Pseudomonas spp., such as P. fluorescens group, P. fragi, and P. putida, are the major psychrophilic spoilage bacteria in the food industry. Bacteriophages (phages) are a promising tool for controlling food-spoilage and food-poisoning bacteria; however, there are few reports on phages effective on food-spoilage bacteria such as Pseudomonas spp. In this study, 12 Pseudomonas phages were isolated from chicken and soil samples. Based on the host range and lytic activity at 30 °C and 4 °C and various combinations of phages, phages vB_PflP-PCS4 and vB_PflP-PCW2 were selected to prepare phage cocktails to control Pseudomonas spp. The phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 showed the strongest lytic activity and retarded regrowth of P. fluorescens and P. putida at 30 °C, 8 °C, and 4 °C at a multiplicity of infection of 100. Nucleotide sequence analysis of the genomic DNA indicated that vB_PflP-PCS4 and vB_PflP-PCW2 phages were lytic phages of the Podoviridae family and lacked tRNA, toxin, or virulence genes. A novel endolysin gene was found in the genomic DNA of phage vB_PflP-PCS4. The results of this study suggest that the phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 is a promising tool for the biocontrol of psychrophilic food-spoilage pseudomonads during cold storage and distribution.

    DOI: 10.1007/s10123-023-00479-2

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  • Screening of genes involved in phage-resistance of Escherichia coli and effects of substances interacting with primosomal protein A on the resistant bacteria

    Lin, CY; Murayama, T; Futada, K; Tanaka, S; Masuda, Y; Honjoh, K; Miyamoto, T

    JOURNAL OF APPLIED MICROBIOLOGY   135 ( 1 )   2024.1   ISSN:1364-5072 eISSN:1365-2672

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    Language:English   Publisher:Journal of Applied Microbiology  

    Aims: The study was to identify the genes involved in phage resistance and to develop an effective biocontrol method to improve the lytic activity of phages against foodborne pathogens. Methods and results: A total of 3,909 single gene-deletion mutants of Escherichia coli BW25113 from the Keio collection were individually screened for genes involved in phage resistance. Phage S127BCL3 isolated from chicken liver, infecting both E. coli BW25113 and O157: H7, was characterized and used for screening. The 10 gene-deletion mutants showed increased susceptibility to phage S127BCL3. Among them, priA gene-deletion mutant strain showed significant susceptibility to the phages S127BCL3 and T7. Furthermore, we investigated the substances that have been reported to inhibit the function of primosomal protein A (PriA) and were used to confirm increased phage susceptibility in E. coli BW25113 (Parent strain) and O157: H7. Conclusion: PriA inhibitors at a low concentration showed combined effects with phage against E. coli O157: H7 and delayed the regrowth rate of phage-resistant cells.

    DOI: 10.1093/jambio/lxad318

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  • Identification of Low Temperature Inducible Genes of Lactuca sativa by Using Suppression Subtractive Hybridization Method

    HONJOH Ken–ichi, OKANO Hitomi, SASAKI Mika, KUROKAWA Masaru, KIMURA Taiki, SHIBATA Kyosuke, MASUDA Yoshimitsu, MIYAMOTO Takahisa

    Journal of the Faculty of Agriculture, Kyushu University   69 ( 1 )   11 - 23   2024   ISSN:00236152

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    Lettuce (Lactuca sativa) is one of important vegetables taken as raw state. It is expected to be kept as high quality under low temperature conditions, especially freezing state. However, there are few research regarding to its molecular response to abiotic stress of lettuce. As we reported previously, lettuce plants acquire low levels of freeze tolerance. In the present paper, we have isolated 192 cDNA clones corresponding to cold induced genes of lettuce plant by using a PCR–based suppression subtractive hybridization method. Most clones were categorized into 62 distinct known genes based on homology search. Out of the corresponding genes, 45 genes were confirmed to be low–temperature–inducible with reverse transcriptionqPCR. Some of the genes encoded stress–related proteins, such as late embryogenesis abundant proteins including dehydrin, which were expected to be involved in enhancement of freezing tolerance. On the other hand, some of proteins encoded by genes were suspected to be involved in suppressing the enhancement of freezing tolerance, such as vacuolar processing enzyme (VPE), adagio protein, and gigantea–like protein. In particular, VPE have been reported to be associated with program cell death, suggesting that it is negatively involved in freezing tolerance of lettuce.

    DOI: 10.5109/7169359

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  • Effects of flavonoids on the phage susceptibility of <i>Escherichia coli</i> and on the transcription of chaperone gene <i>dnaK</i>

    Lin Chen-Yu, Futada Koshiro, Kyaw Phyo Htet Htet, Tanaka Shota, El-Telbany Mohamed, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa

    Food Science and Technology Research   30 ( 2 )   205 - 212   2024   ISSN:13446606 eISSN:18813984

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    Language:English   Publisher:Japanese Society for Food Science and Technology  

    <p>In this study, the <i>dnaK</i> gene-deletion mutant strain of <i>Escherichia coli</i> BW25113 showed a higher susceptibility than the wild-type strain of <i>E. coli</i> BW25113 to phage S127BCL3. Flavonoids, myricetin and quercetin which had been reported to suppress the role of DnaK were tested to examine their effects on the phage susceptibility of <i>E. coli</i> BW25113. A 6-h pretreatment with 500 µmol/L myricetin or quercetin increased the phage susceptibility of <i>E. coli</i> BW25113. A similar result was observed in <i>E. coli</i> O157:H7. Real-time quantitative polymerase chain reaction (qPCR) was conducted to investigate the effects of flavonoids on the transcription of chaperone genes (<i>dnaK, dnaJ, groEL</i>, and <i>grpE</i>) in <i>E. coli.</i> Pretreatment of wild-type <i>E. coli</i> BW25113 with flavonoids decreased the transcription of chaperone genes. This is the first report demonstrating the enhancement of the phage susceptibility of both <i>E. coli</i> BW25113 and <i>E. coli</i> O157:H7 by flavonoids. The results of this study on the combined effects of flavonoids involved in foods and phages on <i>E. coli</i> provide scientific bases for development of a novel biocontrol method of foodborne bacteria.</p>

    DOI: 10.3136/fstr.fstr-d-23-00186

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  • Potential application of phage vB_EfKS5 to control Enterococcus faecalis and its biofilm in food

    El-Telbany Mohamed, Lin Chen-Yu, Abdelaziz Marwa Nabil, Maung Aye Thida, El-Shibiny Ayman, Mohammadi Tahir Noor, Zayda Mahmoud, Wang Chen, Zar Chi Lwin Su, Zhao Junxin, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa, El Mohamed

    AMB Express   13 ( 1 )   2023.11   eISSN:21910855

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    Contaminated food with antibiotic-resistant Enterococcus spp. could be the vehicle for transmitting Enterococcus to humans and accordingly cause a public health problem. The accumulation of biogenic amines produced by Enterococcus faecalis (E. faecalis) in food may have cytological effects. Bacteriophages (phage in short) are natural antimicrobial agents and can be used alone or in combination with other food preservatives to reduce food microbial contaminants. The aim of this study was to isolate a novel phage against E. faecalis and determine its host range to evaluate its potential application. Bacteriophage, vB_EfKS5, with a broad host range, was isolated to control the growth of E. faecalis. The vB_EfKS5 genome is 59,246 bp in length and has a GC content of 39.7%. The computational analysis of phage vB_EfKS5 genome confirmed that it does not contain any lysogenic, toxic, or virulent genes. Phage vB_EfKS5 exhibited lytic activity against most E. faecalis isolates with different multiplicities of infections and it infected 75.5% (22/29) of E. faecalis isolates and 42.3% (3/7) of E. faecium isolates. It was also able to destroy the biofilm formed by E. faecalis with different MOIs. Phage vB_EfKS5 alone or in combination with nisin could control the growth of E. faecalis in broth and milk. Based on its high productivity, stability, short latent period, and large burst size, phage vB_EfKS5 has a high potential for applications both in food and medical applications.

    CiNii Research

  • Potential application of phage vB_EfKS5 to control Enterococcus faecalis and its biofilm in food

    El-Telbany, M; Lin, CY; Abdelaziz, MN; Maung, AT; El-Shibiny, A; Mohammadi, TN; Zayda, M; Wang, C; Lwin, SZC; Zhao, JX; Masuda, Y; Honjoh, KI; Miyamoto, T; El, M

    AMB EXPRESS   13 ( 1 )   130   2023.11   ISSN:2191-0855

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    Contaminated food with antibiotic-resistant Enterococcus spp. could be the vehicle for transmitting Enterococcus to humans and accordingly cause a public health problem. The accumulation of biogenic amines produced by Enterococcus faecalis (E. faecalis) in food may have cytological effects. Bacteriophages (phage in short) are natural antimicrobial agents and can be used alone or in combination with other food preservatives to reduce food microbial contaminants. The aim of this study was to isolate a novel phage against E. faecalis and determine its host range to evaluate its potential application. Bacteriophage, vB_EfKS5, with a broad host range, was isolated to control the growth of E. faecalis. The vB_EfKS5 genome is 59,246 bp in length and has a GC content of 39.7%. The computational analysis of phage vB_EfKS5 genome confirmed that it does not contain any lysogenic, toxic, or virulent genes. Phage vB_EfKS5 exhibited lytic activity against most E. faecalis isolates with different multiplicities of infections and it infected 75.5% (22/29) of E. faecalis isolates and 42.3% (3/7) of E. faecium isolates. It was also able to destroy the biofilm formed by E. faecalis with different MOIs. Phage vB_EfKS5 alone or in combination with nisin could control the growth of E. faecalis in broth and milk. Based on its high productivity, stability, short latent period, and large burst size, phage vB_EfKS5 has a high potential for applications both in food and medical applications.

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  • Isolation, characterization of Enterococcus phages and their application in control of E. faecalis in milk

    Wang, C; Zhao, JX; Lin, YZ; Yuan, L; El-Telbany, M; Maung, AT; Abdelaziz, MNS; Masuda, Y; Honjoh, KI; Miyamoto, T

    JOURNAL OF APPLIED MICROBIOLOGY   134 ( 11 )   2023.11   ISSN:1364-5072 eISSN:1365-2672

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    Aims: Isolation and characterization of Enterococcus phages and application of phage cocktail to control E. faecalis in milk. Methods and Results: For phage isolations, double layer agar method was used. Host range of the phages were determined by the spot test. Twelve phages with varying host ranges were isolated. Phages PEF1, PEF7b, and PEF9 with different host ranges and lytic activities were selected for phage cocktails. Compared to two-phages cocktails tested, the cocktail containing all the three phages displayed stronger antibacterial and biofilm removal activities. The cocktail treatment reduced viable E. faecalis in biofilm by 6 log within 6 h at both 30◦C and 4◦C. In milk, the cocktail gradually reduced the viable count of E. faecalis and the count reached below the lower limit of detection at 48 h at 4◦C. Conclusion: The strong bactericidal and biofilm removal activities of the phage cocktail suggest the potential of this cocktail as a natural biocontrol agent for combating E. faecalis in milk.

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  • Biocontrol of<i> Salmonella</i><i> Typhimurium</i> in milk, lettuce, raw pork meat and ready-to-eat steamed-chicken breast by using a novel bacteriophage with broad host range

    Zhao, JX; Lin, YZ; Wang, C; Zayda, M; Maung, AT; Mohammadi, TN; Duc, HM; Yu, P; Ma, MM; Gong, DM; Sato, J; Masuda, Y; Honjoh, K; Miyamoto, T; Zeng, ZL

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   402   110295   2023.10   ISSN:0168-1605 eISSN:1879-3460

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    Salmonella spp., one of the most frequently reported bacteria, causes foodborne illness and economic losses. Due to the threat of increasing antibiotic resistant foodborne pathogens, application of bacteriophages as novel antibacterial agents in food matrices has become an emerging strategy. In this study, a novel Salmonella phage PS3-1 with high lytic activity against Salmonella Typhimurium was identified from previously isolated phages. PS3-1 belonged to the class Caudoviricetes with a broad host range, and had relatively short latent period (15 min), large burst size (92 PFU/cell), high pH stability (pH 3.0–11.0) and thermal tolerance (4–60 °C). Genome sequencing analysis showed that PS3-1 genome consisted of 107,110 bp DNA, without antibiotic resistance and virulence related genes. The results of growth curve and time-kill assay showed that PS3-1 not only inhibited the growth of S. Typhimurium, but also effectively decreased the viable cell counts (0.30–4.72 log) after 24-h incubation at 7, 25 and 37 °C (P < 0.05). Moreover, >1.28 log of established biofilm cells were effectively removed after 24-h treatment with PS3-1. Besides, PS3-1 significantly reduced the viability of S. Typhimurium in milk, lettuce, raw pork meat and ready-to-eat steamed-chicken breast at different temperatures (P < 0.05). These results demonstrated that PS3-1 may be an excellent antibacterial agent for controlling S. Typhimurium in food industry.

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  • Comparison of prevalence, characterization, antimicrobial resistance and pathogenicity of foodborne Listeria monocytogenes in recent 5 years in Japan Reviewed International journal

    #Aye Thida Maung, #Marwa Nabil Sayed Abdelaziz, @Tahir Noor Mohammadi, #Junxin Zhao, #Mohamed EI-Telbany, @Motokazu Nakayama, @Kaori Matsusita, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Microbial Pathogenesis   183   2023.10

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    https://www.sciencedirect.com/science/article/pii/S0882401023003662

    DOI: https://doi.org/10.1016/j.micpath.2023.106333.

    Other Link: 著作権侵害(出版社等)により不許可

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  • Antimicrobial resistance and biofilm formation of Escherichia coli in a Vietnamese Pangasius fish processing facility Reviewed International journal

    @Phan Nguyen Trang, @Tong Thi Anh Ngoc, Yoshimitsu Masuda, Ken-ichi Hohjoh, Takahisa Miyamoto

    Heliyon   9 ( 10 )   2023.10

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    DOI: https://doi.org/10.1016/j.heliyon.2023.e20727.

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  • Comparison of prevalence, characterization, antimicrobial resistance and pathogenicity of foodborne<i> Listeria</i><i> monocytogenes</i> in recent 5 years in Japan

    Maung, AT; Abdelaziz, MNS; Mohammadi, TN; Zhao, JX; EI-Telbany, M; Nakayama, M; Matsusita, K; Masuda, Y; Honjoh, KI; Miyamoto, T

    MICROBIAL PATHOGENESIS   183   106333   2023.10   ISSN:0882-4010 eISSN:1096-1208

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    This study investigated the prevalence, serotype, antimicrobial resistance (AMR), virulence potential, and biofilm formation of Listeria monocytogenes isolated in 2022 in Japan and compared their profiles with those of isolates in 2012 and 2017. A total of 85 chicken samples were randomly collected from different supermarkets in Fukuoka in 2022. L. monocytogenes were isolated by conventional method and characterized by MALDI-TOF MS. Among 85 samples tested in 2022, 9 (10.6%) were positive for L. monocytogenes and 17 strains were isolated from the positive samples. The isolates were serotyped as 1/2b (41.2%), 3a (29.4%), 3b (23.5%) and 1/2a (5.9%). Antimicrobial susceptibility tests of the 2022 isolates showed susceptibility to majority of the antibiotics, except cefoxitin, oxacillin, and fosfomycin. Compared to the previous surveillance results, the prevalence of L. monocytogenes in 2022 (10.6%) was significantly lower (p < 0.05) than those of the isolates in 2017 (24%) and 2012 (52.9%). The distribution of serotypes 1/2a and 1/2b decreased over time, and serotype 4b was not detected in the 2022 isolates. The proportion of multidrug resistant strains in 2022 (16.7%) was significantly lower than those in 2012 (46.7%) and 2017 (82.6%). Moreover, a total of 36 isolates (12 isolates/ year) were used to detect the virulence genes (hlyA, plcA, clpC, and inlA) and biofilm-forming capacity. Most of the isolates from different years harboured four virulence genes. The biofilm formation of the 2022 isolates was significantly weaker (p < 0.05) than those of the 2012 and 2017 isolates. Thus, despite the low rates of contamination in chicken meat and AMR of the isolates, virulent L. monocytogenes contamination in food should still be acknowledged.

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  • Antimicrobial resistance and biofilm formation of Escherichia coli in a Vietnamese Pangasius fish processing facility

    Trang, PN; Ngoc, TTA; Masuda, Y; Hohjoh, KI; Miyamoto, T

    HELIYON   9 ( 10 )   e20727   2023.10   ISSN:24058440 eISSN:2405-8440

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    This study aimed to investigate the occurrence, antibiotic resistance, and biofilm formation of Escherichia coli in the Vietnamese Pangasius fish processing facility. Among 144 samples including Pangasius fish, wash water, food contact surfaces, and personnel gloves, 18 E. coli isolates was detected and characterized. The E. coli was detected most frequently in wash water samples (22%, 8/36), followed by Pangasius fish (18%, 8/45). According to the antibiotic susceptibility test by the disc diffusion method, isolates showed the highest resistance against sulfamethoxazole/trimethoprim (45%), followed by tetracycline (39%), whereas all the E. coli isolates were susceptible to meropenem and fosfomycin. Notably, 39% of the isolates (7/18) were found to be multidrug resistant while no E. coli isolates were confirmed as extended-spectrum β-lactamase producers by the double-disk synergy test. The potency to form biofilm on the polystyrene surface of E. coli isolates indicated that 44% of the isolates (8/18) were classified as weak, 39% (7/18) as moderate, and 17% (3/18) as strong biofilm formers. Interestingly, multidrug resistant E. coli isolates were observed in moderate and strong biofilm producers. Additionally, either slightly acidic hypochlorous water with 40 mg/L of available chlorine or sodium hypochlorite with 100 mg/L of available chlorine exhibited a significant reduction in biofilm mass and biofilm cells of E. coli isolates. This study may provide helpful information about the actual state of E. coli isolates for effective control in the fish processing plant.

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  • The recognition, control, and stress tolerance of bacterial persister cells

    Masuda Yoshimitsu

    Nippon Shokuhin Kagaku Kogaku Kaishi   70 ( 8 )   311 - 318   2023.8   ISSN:1341027X eISSN:18816681

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    <p>Bacterial persister cells are metabolically dormant cells in which cell activities such as protein synthesis and cell division are somehow drastically slowed down, so that they can survive under intensive antibiotic treatment that targets such cell activities. This review summarizes information about persister cells such as their history, definition, mechanism of formation, threats to clinical environments, and how to control them. In addition to such information, this review also introduces our recent studies on the physical stress tolerance of persister cells and their possible control in food environments. We demonstrated that some bacterial persister cells are indeed highly heat and acid tolerant compared to normal cells, although diversity was discovered in persister cell populations. We also found that indole and its derivatives or acetic acid are promising candidates for persister cell eradication in food environments. We believe that this review will aid in wider attention to and understanding of the importance of these quiet yet clever bacterial persister cells.</p>

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  • Biocontrol of Salmonella Typhimurium in milk, lettuce, raw pork meat and ready-to-eat steamed-chicken breast by using a novel bacteriophage with broad host range Reviewed International journal

    Junxin Zhaoa,, Yunzhi Lin, Chen Wang, Mahmoud Zayd, Aye Thida Maung, Tahir Noor Mohammadi, Hoang Minh Duc, Ping Yu, Maomao Ma, Deming Gong, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Zheling Zeng

    International Journal of Food Microbiology   402   2023.6

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  • The Use of Phage Cocktail and Various Antibacterial Agents in Combination to Prevent the Emergence of Phage Resistance

    Duc, HM; Zhang, Y; Hoang, SM; Masuda, Y; Honjoh, KI; Miyamoto, T

    ANTIBIOTICS-BASEL   12 ( 6 )   2023.6   ISSN:2079-6382

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    Bacterial food poisoning cases due to Salmonella and E. coli O157:H7 have been linked with the consumption of a variety of food products, threatening public health around the world. This study describes the combined effects of a phage cocktail (STG2, SEG5, and PS5), EDTA, nisin, and polylysine against the bacterial cocktail consisting of S. typhimurium, S. enteritidis, and E. coli O157:H7. Overall, phage cocktail (alone or in combination with nisin or/and polylysine) not only showed great antibacterial effects against bacterial cocktail at different temperatures (4 °C, 24 °C, and 37 °C), but also totally inhibited the emergence of phage resistance during the incubation period. These results suggest that the combination of phages with nisin or/and polylysine has great potential to simultaneously control S. typhimurium, S. enteritidis, and E. coli O157:H7.

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  • The Use of Phage Cocktail and Various Antibacterial Agents in Combination to Prevent the Emergence of Phage Resistance Reviewed International journal

    @Duc, Hoang Minh, @Yu Zhang, @Son Minh Hoang, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    Antibiotics   12 ( 6 )   1089   2023.5

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    https://www.mdpi.com/2079-6382/12/6/1077

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  • Biofilm Formation From Listeria monocytogenes Isolated From Pangasius Fish-processing Plants Reviewed International journal

    Phan Nguyen Trang, Tong Thi Anh Ngoc, Yoshimitsu Masuda, Ken-ichi Hohjoh, Takahisa Miyamoto,

    Journal of Food Protection   86 ( 3 )   109802   2023.3

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  • Biofilm Formation From Listeria monocytogenes Isolated From Pangasius Fish-processing Plants Reviewed International journal

    Phan Nguyen Trang, Tong Thi Anh Ngoc, Yoshimitsu Masuda, Ken-ichi Hohjoh, Takahisa Miyamoto

    Journal of Food Protection   86 ( 3 )   - 100044   2023.3

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  • Biofilm Formation From Listeria monocytogenes Isolated From Pangasius Fish-processing Plants

    Trang P.N., Ngoc T.T.A., Masuda Y., Hohjoh K.I., Miyamoto T.

    Journal of Food Protection   26 ( 3 )   2023.3   ISSN:0362028X

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    Biofilm formation of Listeria monocytogenes in food processing environments cause potential source of cross-contamination to foodstuffs; hence, the control of biofilm is currently addressed to find effective solutions for preventing biofilm formation or eliminating the established one. Forty‐five strains of Listeria monocytogenes isolated from Pangasius fish‐processing plants were studied for their capability to form a biofilm on 96‐well microtiter plate by using the conventional crystal violet staining. Additionally, the inhibitory effect of biofilm formation by food additives including monascus pigment and ε‐polylysine was examined. The average OD value showing biofilm mass of all 45 strains L. monocytogenes increased with an increasing temperature and time (p < 0.05). Monascus pigment and ε‐polylysine significantly decreased biofilm formation by 80 ± 5.5% and 20 ± 5.9%, respectively, at the tested concentration (p < 0.05) Further, the effects of lysozyme (0.1 mg/mL) alone or in combination with slightly acidic hypochlorous water (SAHW) with 40 mg/L available chlorine or sodium hypochlorite (NaOCl) with 100 mg/L available chlorine against 7‐d established biofilm of L. monocytogenes were investigated. The results indicated that slightly acidic hypochlorous water alone exhibited significant antibacterial activity (p < 0.05), decreasing the viable count by 5.2 ± 0.5 log CFU/mL. It seems that sequential treatment of lysozyme and SAHW showed an additional efficacy against biofilm of L. monocytogenes on polystyrene plate surface, reducing 70% of biomass of biofilm and 7.6 ± 0.3 log of biofilm viable cells (p < 0.05). Additionally, SAHW exhibited greater bactericidal activity against viable biofilm cells than NaOCl did. This result reveals that SAHW is a promising disinfectant agent against L. monocytogenes and the potential alternative to NaOCl in practice.

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  • Genomic characterization and application of a novel bacteriophage STG2 capable of reducing planktonic and biofilm cells of Salmonella

    Duc, HM; Zhang, Y; Son, HM; Huang, HH; Masuda, Y; Honjoh, KI; Miyamoto, T

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   385   109999   2023.1   ISSN:0168-1605 eISSN:1879-3460

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    As one major foodborne pathogen, Salmonella can cause serious food poisoning outbreaks worldwide. Bacteriophage therapy is increasingly considered as one of the promising antibacterial agents for the biocontrol of foodborne pathogens. In the current study, a lytic phage STG2 capable of infecting S. enteritidis and S. typhimurium was characterized, and its efficacy in reducing these foodborne pathogens in both planktonic and biofilm forms was evaluated on cabbage and various surfaces. Genomic characterization revealed that phage STG2 was Siphoviridae phage (Epseptimavirus genus) with a dsDNA genome comprising of 114,275 bp and its genome does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing (>2 Log) planktonic cells on cabbage as well as the biofilms formed on cabbage, polystyrene, and stainless steel, suggesting that phage STG2 is capable of simultaneously controlling both S. enteritidis and S. typhimurium contaminations on food and food-related surfaces.

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  • Genomic characterization and application of a novel bacteriophage STG2 capable of reducing planktonic and biofilm cells of Salmonella Invited Reviewed International journal

    Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    International Journal of Food Microbiology   2023.1

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  • Mechanism for inhibition of cytotoxicity of Shiga toxin by luteolin Reviewed International journal

    Yuan L, Nakamichi R, Hirata Y, Matsuda A, Shinohara Y, Yamada A, Masuda Y, Honjoh KI, Miyamoto T.

    Toxicol In Vitro   87   108460   2023.1   ISSN:0887-2333 eISSN:1879-3177

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  • Characterization of novel antimicrobial peptides designed on the basis of amino acid sequence of peptides from egg white hydrolysate. International journal

    Cunkuan Shen, Yunzhi Lin, Tahir Noor Mohammadi, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    International journal of food microbiology   378   109802 - 109802   2022.10   ISSN:0168-1605 eISSN:1879-3460

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    Salmonella enterica subsp. enterica serotype Typhimurium (S. Typhimurium) is one of the most prevalent foodborne pathogens responsible for food poisoning and is spread through the consumption of contaminated poultry products. In this study, four antimicrobial peptides (AMPs) with varying hydrophobicity and helical structure-forming tendencies were designed and synthesized based on the amino acid sequences of peptides from egg white hydrolysate. Two of these AMPs, P1R3 (KSWKKHVVSGFFLR) and P1C (KSWKKHVVSGFFLRLWVHKK), exhibited inhibitory activity against S. Typhimurium and compromised its biofilm-forming ability. Investigation of their modes of action revealed that P1R3 and P1C interact with and permeabilize the cytoplasmic membrane of bacteria, leading to membrane potential dissipation, damage to membrane integrity, and consequent bacterial death. P1R3 also bound to S. Typhimurium DNA, resulting in DNA aggregation or precipitation. Moreover, both peptides showed negligible cytotoxicity to Vero cells, and P1C displayed significant antimicrobial activity in chicken meat. Peptides P1R3 and P1C, therefore, have the potential to be developed as promising food preservatives, especially against pathogenic S. Typhimurium.

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  • Slightly acidic hypochlorous water effective against dual-species biofilm of Listeria monocytogenes and Escherichia coli strains isolated from Pangasius fish-processing plants Reviewed International journal

    Trang Nguyen Phan, Anh Ngoc Tong Thi, Yoshimitsu Masuda, Ken-ichi Hohjoh, Takahisa Miyamoto

    Food Science and Technology Research   28 ( 6 )   141 - 149   2022.9

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  • Characterization and optimization of bacteriophage cocktails to control Clostridium perfringens in vitro and in curry roux Reviewed International journal

    Tahir Noor Mohammadi, Cunkuan Shen, Yuncheng Li, Mahmoud Gamaleldin Zayda, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    International Journal of Food Microbiology   380   109440   2022.8

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  • Characterization of novel antimicrobial peptides designed on the basis of amino acid sequence of peptides from egg white hydrolysate Reviewed International journal

    Cunkuan Shen, Yunzhi Lin, Tahir Noor Mohammadi, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    International Journal of Food Microbiology   378   2022.6

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  • Inactivation of mixed Escherichia coli O157:H7 biofilms on lettuce by bacteriophage in combination with slightly acidic hypochlorous water (SAHW) and mild heat treatment Reviewed International journal

    Zhang, Y., Huang, H.-H., Ma, L.Z., Masuda, Y., Honjoh, K.-I., Miyamoto, T.

    Food Microbiology   104   2022.6

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  • Inactivation of mixed Escherichia coli O157:H7 biofilms on lettuce by bacteriophage in combination with slightly acidic hypochlorous water (SAHW) and mild heat treatment. International journal

    Yu Zhang, Hung-Hsin Huang, Luyan Z Ma, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    Food microbiology   104   104010 - 104010   2022.6   ISSN:0740-0020 eISSN:1095-9998

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    Escherichia coli O157:H7 is one of the most important foodborne pathogens that can persist in leafy green vegetables and subsequently produce biofilms. Biofilm formation is an ongoing concern in the food industry as biofilms are relatively resistant to a variety of antimicrobial treatments. In the present study, we evaluated the combined effects of phage FP43 and mild-heated slightly acidic hypochlorous water (SAHW) in reducing established biofilms on lettuce. Prior to the sequential treatments involving phage-SAHW and SAHW-phage for long-term storage, equal inoculum densities of E. coli O157:H7 and E. coli O91:H- were added on iceberg lettuce surfaces and the lettuce samples were stored at 10 °C for 48 h to allow biofilm formation. The sequential treatment with phage FP43 and SAHW significantly decreased the number of adhered cells, especially the combination of phage FP43 at 25 °C for 2 h and mild-heated SAHW, which considerably eliminated E. coli viable biofilm cells to undetectable levels (>3 log CFU/piece). However, the biofilms were not completely removed, as evidenced via SEM observation. Additionally, sequential treatment with SAHW and phage caused continuous reductions in viable counts, decreasing the viability of E. coli O157:H7 and total E. coli to the lower limit of detection after incubation for 5 d. Meanwhile, bacterial regrowth was observed after treatment with SAHW alone. These results indicated that the combination of phage and SAHW could be considered as a promising strategy to control the formation of E. coli O157:H7 biofilms on lettuce.

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  • Characteristics of Bacterial Persister and Its Control

    MASUDA Yoshimitsu

    KAGAKU TO SEIBUTSU   60 ( 5 )   232 - 239   2022.5   ISSN:0453073X eISSN:18836852

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  • Occurrence, antimicrobial resistance, and genetic diversity of Listeria monocytogenes at fish-processing plants in Vietnam Reviewed International journal

    Trang Nguyen Phan, Takahisa Miyamoto, Yoshimitsu Masuda, Ken-ichi Hohjoh, Anh Ngoc Tong Thi

    Food Science and Technology Research   28 ( 2 )   45 - 52   2022.1

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    DOI: DOI: 10.3136/fstr.FSTR-D-21-00195

  • Characterization of Clostridium perfringens bacteriophages and their application in chicken meat and milk Reviewed International journal

    Tahir Noor Mohammadi, Cunkuan Shen, Yuncheng Li, Mahmoud Gamaleldin Zayda, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY   361   109446 - 109446   2022.1   ISSN:0168-1605 eISSN:1879-3460

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    Clostridium perfringens is one of the most important foodborne pathogens in developed countries. It causes severe food poisoning outbreaks worldwide, along with mortality and economic losses. Recently, bacteriophages have been investigated as an alternative tool to control pathogenic bacteria in the food industry. In this study, 19 Clostridium perfringens and 6 Clostridium perfringens bacteriophages were isolated from chicken meat. According to host range and stability tests, bacteriophage CPQ1 showed high thermostability and the broadest host range. The electron micrograph image of this bacteriophage suggested that it belongs to the Picovirinae subfamily of the Podoviridae family. Nucleotide sequence analysis of the genomic DNA indicated the absence of any antibiotic resistance, toxin, or virulence genes. In broth, CPQ1 showed strong lytic activity with a low MOI of 1, decreasing the OD600 of Clostridium perfringens cell suspension from 0.2 to 0.02 at 37 °C in 2 h. In pasteurized milk and chicken meat, CPQ1 with an MOI of 10 also caused a significant decrease in viable counts of Clostridium perfringens compared to the bacteriophageless control at both 24 °C and 37 °C. This is the first report on the application of bacteriophage to control Clostridium perfringens in foods.

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  • Application of endolysin LysSTG2 as a potential biocontrol agent against planktonic and biofilm cells of <i>Pseudomonas</i> on various food and food contact surfaces

    Zhang, Y; Huang, HH; Duc, HM; Masuda, Y; Honjoh, K; Miyamoto, T

    FOOD CONTROL   131   2022.1   ISSN:0956-7135 eISSN:1873-7129

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    Publisher:Food Control  

    The gene encoding LysSTG2, a novel endolysin from Salmonella-lytic bacteriophage STG2, has been cloned, overexpressed, and characterized previously. In this study, LysSTG2 was used to control Pseudomonas aeruginosa and P. putida, which showed high sensitivity to LysSTG2, in bottled water, milk, chicken breast, salmon, and the biofilms formed on the surface of polystyrene resin and stainless steel. In bottled water, LysSTG2 combined with EDTA reduced the viable counts of P. aeruginosa and P. putida by 2.2 log and to lower than the limit of detection, respectively. However, there was no significant decrease in viable counts in pasteurized milk artificially contaminated with these bacteria. Meanwhile, the addition of 1 mg/mL LysSTG2 alone reduced the viable counts of P. aeruginosa and P. putida by 0.6 log in chicken and 1.1 log in salmon samples contaminated with these bacteria, respectively. Further reduction in viable counts in combination with EDTA was not observed. Additionally, a strong effect of LysSTG2 on the removal of P. aeruginosa and P. putida biofilms was observed on the polystyrene resin and stainless steel surfaces, displaying more than 99.9% decrease in viable biofilm cells after 2 h of treatment at 37 °C. The results of this study suggest that LysSTG2 has the potential to control both planktonic and biofilm cells of Pseudomonas. Further investigations are required to optimize and improve the use of the endolysin for application in food and food processing facilities.

    DOI: 10.1016/j.foodcont.2021.108460

    Web of Science

    Scopus

  • Slightly acidic hypochlorous water effective against dual-species biofilm of <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> strains isolated from <i>Pangasius</i> fish-processing plants

    Phan Trang Nguyen, Thi Anh Ngoc Tong, Masuda Yoshimitsu, Hohjoh Ken-ichi, Miyamoto Takahisa

    Food Science and Technology Research   28 ( 6 )   521 - 527   2022   ISSN:13446606 eISSN:18813984

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    Language:English   Publisher:Japanese Society for Food Science and Technology  

    <p>This study illustrates the effectiveness of slightly acidic hypochlorous water (SAHW) in comparison with sodium hypochlorite (NaOCl) in reducing biomass and viable cells in biofilms established by the dual species, <i>Listeria monocytogenes</i> and <i>Escherichia coli</i>, on a microtiter plate and stainless-steel coupon. The SAHW and NaOCl treatments exhibited significant efficacy against biofilms (<i>p</i> < 0.05) on both surfaces. Additionally, compared with NaOCl treatment, SAHW treatment significantly reduced biofilm formation (<i>p</i> < 0.05). With its high antibiofilm activity, SAHW not only reduced the biomass of biofilms, but also significantly decreased viable biofilm cells to 5 log CFU/mL or ≤1 log CFU/cm2 on microtiter plates and stainless-steel surfaces, respectively. These results indicate that SAHW is a potential candidate for disinfectants against biofilms on various food contact surfaces.</p>

    DOI: 10.3136/fstr.fstr-d-22-00074

    Web of Science

    Scopus

    CiNii Research

  • Occurrence, antimicrobial resistance, and genetic diversity of <i>Listeria monocytogenes</i> at fish-processing plants in Vietnam

    Phan Trang Nguyen, Miyamoto Takahisa, Masuda Yoshimitsu, Hohjoh Ken-ichi, Thi Anh Ngoc Tong

    Food Science and Technology Research   28 ( 2 )   141 - 149   2022   ISSN:13446606 eISSN:18813984

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    Language:English   Publisher:Japanese Society for Food Science and Technology  

    <p>In this study, the occurrence, serogroups, virulence genes, antibiotic susceptibility profiles, and diversity were estimated for <i>Listeria monocytogenes</i> isolated from <i>Pangasius</i> fish in two processing facilities in Vietnam. <i>L. monocytogenes</i> was most frequently detected in wash water samples at 20.8% (15 out of 72), and in <i>Pangasius</i> fish it was 16.6% (15 out of 90 fish samples). Serotypes 1/2b were dominant, and internalin genes (<i>inlA, inlC</i>, and <i>inlJ</i>) were found in all the <i>L. monocytogenes</i> isolates. Most of the isolates were resistant to cefoxitin, oxacillin, and fosfomycin. Genotyping of <i>L. monocytogenes</i> by random amplified polymorphic DNA analysis revealed three clusters of the isolates specific to the facilities to some extent. This study suggests a high risk of <i>L. monocytogenes</i> contamination from wash water to fish. Hence, maintaining the quality of water and designing sanitation procedures to reduce <i>L. monocytogenes</i> contamination are required to ensure food safety at <i>Pangasius</i> fish processing plants.</p>

    DOI: 10.3136/fstr.fstr-d-21-00195

    Web of Science

    Scopus

    CiNii Research

  • Characterization and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from chicken and pork Invited Reviewed International journal

    Khin Zar Linn, Munenori Furuta, Motokazu Nakayama, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    International Journal of Food Microbiology   360   2021.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: DOI: 10.1016/j.ijfoodmicro.2021.109440

  • Endolysin LysSTG2: Characterization and application to control Salmonella Typhimurium biofilm alone and in combination with slightly acidic hypochlorous water Reviewed International journal

    Yu Zhang, Hung-Hsin Huang, Hoang Minh Duc, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Food Microbiology   98   854 - 859   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.fm.2021.103791

  • Endolysin LysSTG2: Characterization and application to control Salmonella Typhimurium biofilm alone and in combination with slightly acidic hypochlorous water Reviewed International journal

    Yu Zhang, Hung-Hsin Huang, Hoang Minh Duc, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Food Microbiology   98   2021.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.fm.2021.103791

  • Inhibition of phage-resistant bacterial pathogen re-growth with the combined use of bacteriophages and EDTA Reviewed International journal

    100   2021.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.fm.2021.103853

  • Effect of Selected Food Additives on Biofilm Formation by Foodborne Pathogens on Stainless Steel

    Cunkuan Shen, Chikako Machida, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    66 ( 1 )   2021.4

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    Language:English   Publishing type:Research paper (bulletin of university, research institution)  

    DOI: 10.5109/4363550

  • Effects of bacteriophage on inhibition and removal of mixed biofilm of enterohemorrhagic Escherichia coli O157:H7 and O91:H- Reviewed International journal

    Yu Zhang, Kumiko Shigemura, Hoang Minh Duc, Cunkuan Shen, Hung-Hsin Huang, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    LWT - Food Science and Technology   134   903 - 913   2020.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.lwt.2020.109945

  • Effect of epigallocatechin gallate on gene expression of Staphylococcus aureus Reviewed International journal

    Apisada Kitichalermkiat, Mao Katsuki, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    Journal of Global Antimicrobial Resistance   22   49 - 57   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jgar.2020.06.006

  • Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157:H7 in different food matrices Reviewed

    Hoang Minh Duc, Hoang Minh Son, Hazel Pang Shu Yi, Jun Sato, Pham Hong Ngan, Yoshimitsu Masuda, Ken ichi Honjoh, Takahisa Miyamoto

    Food Research International   131   194 - 200   2020.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.foodres.2020.108977

  • Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese Reviewed

    Mahmoud Ge Zayda, Yoshimitsu Masuda, Ahmed M. Hammad, Ken ichi Honjoh, Abdelrahman M. Elbagory, Takahisa Miyamoto

    International Dairy Journal   104   633 - 640   2020.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.idairyj.2020.104646

  • Transcriptional changes involved in inhibition of biofilm formation by ε-polylysine in Salmonella Typhimurium Reviewed

    277 - 285   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism underlying ε-polylysine-mediated inhibition of biofilm formation, the transcriptional profiles of ε-polylysine-treated and untreated Salmonella Typhimurium cells were comparatively analysed. The genome-wide DNA microarray analysis was performed using Salmonella Typhimurium incubated with 0.001% ε-polylysine in 0.1% Bacto Soytone at 30 °C for 2 h. The expression levels of genes involved in curli amyloid fibres and cellulose production, quorum sensing, and flagellar motility were downregulated, whereas those of genes associated with colanic acid synthesis were upregulated after treatment with ε-polylysine. The microarray results were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore, treatment with ε-polylysine decreased the production of colanic acid in Salmonella Typhimurium. The findings of this study improved our understanding of the mechanisms underlying ε-polylysine-mediated biofilm inhibition and may contribute to the development of new disinfectants to control biofilm during food manufacturing and storage.

    DOI: 10.1007/s00253-020-10575-2

  • Effects of Sucrose on Heat Resistance and Gene Expression in Salmonella Typhimurium Invited Reviewed International journal

    FOOD SCIENCE AND TECHNOLOGY RESEARCH   25 ( 6 )   10259 - 10271   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3136/fstr.25.903

  • Antimicrobial resistance profiles of Listeria monocytogenes isolated from chicken meat in Fukuoka, Japan Reviewed

    Aye Thida Maung, Tahir Noor Mohammadi, Satoko Nakashima, Pei Liu, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    International Journal of Food Microbiology   304   17 - 25   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ijfoodmicro.2019.05.016

  • Baicalein inhibits Stx1 and 2 of EHEC Effects of baicalein on the cytotoxicity, production, and secretion of shiga toxins of enterohaemorrhagic escherichia coli Reviewed

    Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Hoang Minh Duc, Motokazu Nakayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken Ichi Honjoh, Takahisa Miyamoto

    Toxins   11 ( 9 )   70 - 79   2019.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/toxins11090505

  • Transcriptional analysis on heat resistance and recovery from thermal damage in Salmonella under high salt condition Reviewed

    Xiaowen Cui, Chuanqi Hu, Liushu Ou, Yumiko Kuramitsu, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    LWT   106   291 - 300   2019.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.lwt.2019.02.056

  • Mechanism for antibacterial action of epigallocatechin gallate and theaflavin-3,3′-digallate on Clostridium perfringens Reviewed

    126 ( 2 )   6647 - 6655   2019.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    Aim: The purpose of this study was to clarify the mechanism of the antibacterial action of two high potential and natural food additives, epigallocatechin gallate (EGCg) and theaflavin-3,3′-digallate (TF3), on Clostridium perfringens. Methods and Results: Minimal inhibitory concentrations were determined by the serial dilution method. Afterwards, the cells were treated with 250 or 1000 mg l
    −1
    of EGCg and 125 or 500 mg l
    −1
    of TF3 and morphological changes were observed and cell sizes were also measured under fluorescence microscopy. Our results showed that TF3 had a twice stronger antibacterial activity than EGCg against C. perfringens. Phase-contrast and fluorescence microscopy confirmed that the bacterial cells elongated without DNA segregation and septum formation in the presence of 250 mg l
    −1
    EGCg. While in the higher concentration of EGCg and TF3, cell growth was suppressed. Bacterial cells reached to around 12 μm after the 24 h incubation with 250 mg l
    −1
    EGCg, but the cells were shorter than the control at 1000 mg l
    −1
    of EGCg. After washing and incubating the elongated cells in fresh medium, DNA segregated at 2 h of incubation. The average cell length decreased gradually and reached the normal size at 8 h. Conclusion: It seems that EGCg at a low concentration affected the proteins involved in the septum formation, DNA segregation and cell division. Furthermore, the high concentration of EGCg and TF3 seemed to cause stronger cellular damage to C. perfringens. Significance and Impact of the Study: These polyphenols are widely distributed in all higher plants especially in tea plants, and people tend to use natural food additives rather than synthetic ones. EGCg and TF3, as natural food additives, can prevent C. perfringens food poisoning along with other potential health benefits.

    DOI: 10.1111/jam.14134

  • Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus Reviewed

    Apisada Kitichalermkiat, Masahiro Kurahachi, Ai Nonaka, Motokazu Nakayama, Kanami Shimatani, Naofumi Shigemune, Takashi Tsugukuni, Jun Hitomi, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    Food Science and Technology Research   25 ( 2 )   158 - 164   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3136/fstr.25.277

  • Application of bacteriophages in simultaneously controlling Escherichia coli O157:H7 and extended-spectrum beta-lactamase producing Escherichia coli Reviewed

    Hoang Minh Son, Hoang Minh Duc, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    Applied Microbiology and Biotechnology   102 ( 23 )   4503 - 4508   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00253-018-9399-1

  • Role of phage shock protein in recovery of heat-injured salmonella Reviewed

    Xiaowen Cui, Hsu Ming Sherman Wen, Yoshimasa Kinoshita, Shota Koishi, Chika Isowaki, Liushu Ou, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto

    Biocontrol Science   23 ( 1 )   490 - 496   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.4265/bio.23.17

  • Nutrition-adaptive control of multiple-bacteriocin production by Weissella hellenica QU 13 Reviewed

    Yoshimitsu Masuda, R. H. Perez, Takeshi Zendo, Kenji Sonomoto

    Journal of Applied Microbiology   120 ( 1 )   1245 - 1247   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jam.12997

  • Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin Reviewed

    Rodney H. Perez, Naoki Ishibashi, Tomoko Inoue, Kohei Himeno, Yoshimitsu Masuda, Narukiko Sawa, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Journal of Bacteriology   198 ( 2 )   947 - 953   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JB.00692-15

  • Gene cluster responsible for secretion of and immunity to multiple bacteriocins, the NKR-5-3 enterocins Reviewed

    Naoki Ishibashi, Kohei Himeno, Yoshimitsu Masuda, Rodney Honrada Perez, Shun Iwatani, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

    Applied and Environmental Microbiology   80 ( 21 )   3 - 12   2014.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.02312-14

  • Biological function of a DUF95 superfamily protein involved in the biosynthesis of a circular bacteriocin, leucocyclicin Q Reviewed

    Fuqin Mu, Yoshimitsu Masuda, Takeshi Zendo, Hiroshi Ono, Hiroshi Kitagawa, Haruo Ito, Jiro Nakayama, Kenji Sonomoto

    Journal of Bioscience and Bioengineering   117 ( 2 )   99 - 108   2014.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2013.06.023

  • Zirex A novel zinc-regulated expression system for Lactococcus lactis Reviewed

    Applied and Environmental Microbiology   79 ( 14 )   8164 - 8170   2013.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.00866-13

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Presentations

  • Study on control of Salmonella Persisters by using the antimicrobial peptide, ɛ-Poly-L-Lysine

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • 大腸菌ファージECP52の可変的宿主特異性と宿主認識構造に関する研究

    末安 華,益田 時光,本城 賢一,宮本 敬久

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • Leaderless bacteriocin producing phage against enterohemorrhagic Escherichia coli International conference

    Masuda Y., Uedoi T., Mohammadi T. M., Honjoh K., Miyamoto T.

    FEMS2023  2023.7 

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    Event date: 2023.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Germany  

  • 黄色ブドウ球菌を標的宿主とするリーダーレスバクテリオシン産生ファージの構築とその利用

    木下慧斗,井門さら,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • 食中毒細菌のPersister細胞の熱耐性とその制御に関する研究

    小原愛弥,力武舞夢,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • LLB ファージによるによる腸管出血性大腸菌などの食中毒細菌の制御

    大門亮太,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • 腸管出⾎性⼤腸菌を標的宿主としたLLBファージの構築

    益田時光、上土井達哉、本城賢一、宮本敬久

    九州微⽣物研究フォーラム2022  2022.9 

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:⻑崎⼤学医学部ポンぺ会館(長崎市)   Country:Japan  

  • LLB産生ファージを利用した食中毒細菌の制御

    益田時光、川畑諄、井門さら、上土井達哉、本城賢一、宮本敬久

    日本ファージセラピー研究会 第1回研究集会  2021.11 

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    Event date: 2021.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

  • リーダーレスバクテリオシン産生バクテリオファージの構築

    益田時光、川畑淳、上土井達哉、本城賢一、宮本敬久

    九州微生物研究フォーラム  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島県医師会館4F大ホール(鹿児島市)   Country:Japan  

  • 病原性大腸菌のPersister細胞のストレス耐性とその制御に関する研究

    力武舞夢,益田時光,坂本英莉花,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 病原性大腸菌を標的宿主としたリーダーレスバクテリオシン産生ファージの構築とその利用に関する研究

    上土井達哉,川畑諄,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • CRISPR -Cas10 システムによる,黄色ブドウ球菌を標的宿主としたLLB ファージの構築とその利用

    井門さら,木下慧斗,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Construction Of Leaderless-bacteriocin-producing Phage To Control Both Gram-negative And -positive Pathogenic Bacteria International conference

    Yoshimitsu Masuda, Shun Kawabata, Tatsuya Uedoi, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • ROLE OF YAFQ-DINJ TOXIN-ANTITOXIN SYSTEM AND INDOLE SYNTHESIS BY TNAA IN HEAT TOLERANCE OF ESCHERICHIA COLI International conference

    2018.6 

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    Event date: 2019.9

    Language:English  

    Venue:Berlin   Country:Germany  

  • Isolation and application of broad-host range bacteriophages to control Salmonella Enteritidis, Salmonella Typhimurium, and Escherichia coli O157:H7 simultaneously in in vitro and food matrices International conference

    #Hoang Minh Duc, #Hoang Minh Son, #Pang Shu Yi Hazel, @Yoshimitsu Masuda, @Ken-ichi Honjoh, @Takahisa Miyamoto

    26th International ICFMH Conference FoodMicro2018  2018.6 

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    Event date: 2019.9

    Language:English  

    Country:Germany  

  • リーダーレスバクテリオシン産生ファージの構築

    川畑諄、益田時光、本城賢一、宮本敬久

    公益社団法人 日本食品科学工学会 第66回大会  2019.8 

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    Event date: 2019.8

    Language:Japanese  

    Venue:藤女子大学北16条キャンパス(札幌市)   Country:Japan  

  • Construction of leaderless bacteriocin producing phage International conference

    Yoshimitsu Masuda, Shun Kawabata, Ken-ichi Honjoh, Takahisa Miyamoto

    FEMS 2019 8th Congress of European Microbiologists  2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • 細菌の熱耐性におけるToxin-Antitoxinシステムの機能

    益田 時光, 坂本 英莉花, 本城 賢一, 宮本 敬久

    日本農芸化学会2018年度大会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学(愛知県名古屋市)   Country:Japan  

  • 細菌の熱耐性、損傷菌発生におけるTAシステムの機能の解明

    益田 時光, 坂本 英莉花, 本城 賢一, 宮本 敬久

    第113回日本食品衛生学会学術講演会  2017.11 

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    Event date: 2017.11

    Language:Japanese  

    Venue:タワーホール船堀(東京都江戸川区)   Country:Japan  

  • Role of Toxin-Antitoxin System in Bacterial Heat Injury and Recovery International conference

    Yoshimitsu Masuda, Sae Futatsuishi, Hoang Minh Duc, Aya Nakayama, Ken-ichi Honjoh, Takahisa Miyamoto

    asm microbe 2017  2017.6 

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    Event date: 2017.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  • Study on mechanism for heat resistance of Salmonella in low moisture condition International conference

    Xiaowen Cui, Yumiko Kuramitsu, Liushu Ou, Yue Guo, Chika Isowaki, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    asm microbe 2017  2017.6 

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    Event date: 2017.6

    Language:English  

    Country:United States  

  • Characterization and evaluation of antimicrobial activity of Campylobacter jejuni specific pahges

    Su Zar Chi Lwin, Takayuki Nasu, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 植物ポリフェノールの腸管出血性大腸菌Stx活性阻害機構に関する研究

    松田 采佳,中道 璃名子,益田 時光,本城 賢一,宮本 敬久

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • ダイコンおろしによるキュウリ浅漬中の微生物制御効果に関する研究

    水沼 忍,佐藤 洋介,中山 素一,益田 時光,本城 賢一,宮本 敬久

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • 抗菌剤使用によるレタス栽培土壌中の耐性菌ならびに菌叢の変化に関する研究

    中尾 朱李,安留 正倫,實政 裕哉,益田 時光,本城 賢一,木嶋 伸行,石井 孝昭,宮本 敬久

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • Study on control of phage-resistant Escherichia coli by inhibiting PriA function

    Lin Chen Yu, Masuda Yoshimitsu, Honjoh Ken-ichi ,Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Effects of EGCg and Theaflavins on Bacillus cereus

    Lu Yuan, Yoshimitsu Masuda ,Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • A 5-year difference surveillance on occurrence, antimicrobial resistance and biofilm formation of Listeria monocytogenes from chicken meats in Fukuoka, Japan

    Aye Thida Maung, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Biocontrol of Salmonella Typhimurium in milk, lettuce, raw pork meat and ready-to-eat chicken breast by using a novel bacteriophage with broad host range

    Junxin Zhao, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Detection of Antimicrobial Resistance and Pathogenicity of Staphylococcus aureus isolated from Chicken Samples in Fukuoka, Japan

    Win May Thu Zar, Masuda Yoshimitsu, Honjoh Ken-ichi , Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Study on control of Salmonella Persisters by using the antimicrobial peptide, ɛ-Poly-L-Lysine

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:English  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • Isolation and bio-control of pathogenic Enterococcus. faecalis by bacteriophage cocktail and endolysins

    Chen Wang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Antibacterial effect and mechanism of theaflavin 3,3′-digallate against Listeria monocytogenes

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:English  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • Bacillus cereus Isolated from Foodstuffs in Japan: Incidence, Virulence Genes, Genetic Diversity, and Antibiotic Resistance Between Past and Present

    Marwa Abdelaziz, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Potential application of phage alone or in combination with nisin to control Enterococcus faecalis in food

    MOHAMED ELTELBANY, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Characterization and evaluation of antimicrobial activity of Campylobacter jejuni specific pahges

    Su Zar Chi Lwin, Takayuki Nasu, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

     More details

    Event date: 2023.8

    Language:English  

    Country:Japan  

  • Biocontrol of Salmonella Typhimurium in milk, lettuce, raw pork meat and ready-to-eat chicken breast by using a novel bacteriophage with broad host range

    Junxin Zhao, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Detection of Antimicrobial Resistance and Pathogenicity of Staphylococcus aureus isolated from Chicken Samples in Fukuoka, Japan

    Win May Thu Zar, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Antibacterial effect and mechanism of theaflavin 3,3′-digallate against Listeria monocytogenes

    日本食品科学工学会 第70回記念大会  2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Venue:京都女子大学(京都市東山区今熊野北日吉町35番地)   Country:Japan  

  • Bacillus cereus Isolated from Foodstuffs in Japan: Incidence, Virulence Genes, Genetic Diversity, and Antibiotic Resistance Between Past and Present

    Marwa Abdelaziz, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • A 5-year difference surveillance on occurrence, antimicrobial resistance and biofilm formation of Listeria monocytogenes from chicken meats in Fukuoka, Japan

    Aye Thida Maung, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Potential application of phage alone or in combination with nisin to control Enterococcus faecalis in food

    MOHAMED ELTELBANY, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Study on control of phage-resistant Escherichia coli by inhibiting PriA function

    Lin Chen Yu, Masuda Yoshimitsu, Honjoh Ken-ichi, Miyamoto Takahisa

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Effects of EGCg and Theaflavins on Bacillus cereus

    Lu Yuan, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Isolation and bio-control of pathogenic Enterococcus. faecalis by bacteriophage cocktail and endolysins

    Chen Wang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2023.8 

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    Event date: 2023.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Characteriza on and an bacterial effect of bacteriophage cocktails and endolysin to biocontrol pathogenic Enterococcus faecali International conference

    Chen Wang

    FEMS2023  2023.7 

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    Event date: 2023.7

    Language:English  

    Country:Germany  

  • A study on the control of phage-resistant Escherichia coli by inhibition the priA gene product International conference

    Neo Chen-Yu Lin

    FEMS2023  2023.7 

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    Event date: 2023.7

    Language:English  

    Country:Germany  

  • Antibacterial Effect and Application of a New Bacteriophage Against Salmonella Typhimurium in Milk, Lettuce and Pork International conference

    Junxin Zhao

    2023.7 

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    Event date: 2023.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Prevalence, Serotyping, Antimicrobial Resistance, VirulenceGenes, and Biofi lm Formation of Food-Borne Listeriamonocytogenes in Recent Five Years in Japan International conference

    Aye Thida Maung

    2023.7 

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    Event date: 2023.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Prevalence, Toxin profi le, Antimicrobial Resistance andGenotyping Diversity Among Bacillus cereus Isolated FromVarious Foods in Japan International conference

    Marwa Nabil Sayed Abdelaziz

    2023.7 

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    Event date: 2023.7

    Language:English  

    Country:Korea, Republic of  

  • Effects Of Polyphenols On Viability And Spores Of Bacillus cereus International conference

    Lu Yuan

    2023.7 

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    Event date: 2023.7

    Language:English  

    Country:Korea, Republic of  

  • ルテオリンの志賀毒素活性阻害機構に関する研究

    松田采佳,中道璃名子,平田夕夏, 坂上和,中道璃名子,篠原由依,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • レタスの低温処理によるフラボノイド合成酵素遺伝子群の発現誘導について

    寺本紡乃,益田時光,山田直隆,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • 農薬散布履歴の異なる栽培土壌からの薬剤耐性菌の分離と関連する耐性遺伝子の検索

    中尾朱李,香坂拓哉,益田時光,本城賢一,木嶋伸行,石井孝昭,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • レタス低温誘導性親水性タンパク質の抗菌活性に関する研究

    新名祐作,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • 野菜種子およびスプラウト由来新規抗菌性タンパク質の探索

    中川義健,實政裕哉,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • 空心菜スプラウト由来抗菌タンパク質の分画

    實政裕哉,中川義健,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • Isolation and characterization of phages specific to Lactobacillus bacteria

    2022.11 

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    Event date: 2022.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • リステリアに対するテアフラビン3,3’-ジ-O-ガレートの抗菌作用および抗菌機構の解明

    林云志,益田時光,本城賢一,宮本敬久

    2022年度 (公社)日本食品科学工学会西日本支部および(公社)日本栄養·食糧学会九州·沖縄支部合同大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島大学郡元キャンパス(鹿児島市)   Country:Japan  

  • Multidrug-Resistance and Enterotoxin Production of Coagulase Negative Staphylococci Causing Bovine Subclinical Mastitis in Egypt International conference

    Mahmoud Ge. Zayda, Yoshimitsu Masuda, Tahir. N. Mohammadi, Ahmed M. Hammad, Ken-ichi Honjoh, Abdelrahman M. Elbagory, Takahisa Miyamoto

    ASM MICROBE 2022  2022.6 

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    Event date: 2022.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Characterization And Efficiency Of The Bacteriophage Cocktails To Control Clostridium Perfringens In Vitro And In Curry Roux International conference

    Tahir Noor Mohammadi, Yuncheng Li, Cunkuan Shen, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    ASM MICROBE 2022  2022.6 

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    Event date: 2022.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • 生鶏肉由来Pseudomonas溶菌ファージの特性

    城野大輔、益田時光、本城賢一、宮本敬久

    日本ファージセラピー研究会 第1回研究集会  2021.11 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン開催   Country:Japan  

  • Application of the bacteriophage cocktails to control Clostridium perfringens in food

    Tahir Noor Mohammadi, Yuncheng Li, Cunkuan Shen, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • PriAの機能阻害に基づく大腸菌バクテリオファージ耐性菌集団の制御に関する研究

    村山友香,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • PriAの機能阻害に基づく大腸菌バクテリオファージ耐性菌集団の制御に関する研究

    村山友香,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Study on control of bacteriophage-resistant bacterial populations

    Phyo Htet Htet Kyaw, Tomoka Murayama, Shota Tanaka, Koshiro Futada, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Study on control of bacteriophage-resistant bacterial populations

    Phyo Htet Htet Kyaw, Tomoka Murayama, Shota Tanaka, Koshiro Futada, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • The characterization of endolysin LysSTG2 and its antibacterial activity against Gram-negative bacteria

    Yu Zhang, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • The characterization of endolysin LysSTG2 and its antibacterial activity against Gram-negative bacteria

    Yu Zhang, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Purification and characterization of anti-biofilm peptides from hydrolysate of egg white(Gallus domesticus)

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:English   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • Purification and characterization of anti-biofilm peptides from hydrolysate of egg white(Gallus domesticus)

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • レタス(Lactuca sativa L.)由来の低温誘導性遺伝子の解析およびLsVPEノックアウトレタスの作出

    柴田恭輔,木村太喜,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • レタス(Lactuca sativa L.)由来の低温誘導性遺伝子の解析およびLsVPEノックアウトレタスの作出

    柴田恭輔,木村太喜,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • レタスの低温処理によるフラボノイド関連遺伝子群の発現誘導について

    寺本紡乃,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • レタスの低温処理によるフラボノイド関連遺伝子群の発現誘導について

    寺本紡乃,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 植物ポリフェノールによる腸管出血性大腸菌の病原性抑制機構

    平田夕夏,坂上和,中道璃名子,篠原由依,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 植物ポリフェノールによる腸管出血性大腸菌の病原性抑制機構

    平田夕夏,坂上和,中道璃名子,篠原由依,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 病原性大腸菌を標的宿主としたリーダーレスバクテリオシン産生ファージの構築とその利用に関する研究

    上土井達哉,川畑諄,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • CRISPR -Cas10 システムによる,黄色ブドウ球菌を標的宿主としたLLB ファージの構築とその利用

    井門さら,木下慧斗,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄色ブドウ球菌を標的宿主とするaureocin A53 産生ファージの構築

    木下慧斗,井門さら,上土井達哉,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 黄色ブドウ球菌を標的宿主とするaureocin A53 産生ファージの構築

    木下慧斗,井門さら,上土井達哉,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 新規Pseudomonas溶菌ファージの食品からの分離とその特性解析

    城野大輔,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • グラム陽性食中毒細菌Persisterのストレス耐性

    小原愛弥,力武舞夢,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • グラム陽性食中毒細菌Persisterのストレス耐性

    小原愛弥,力武舞夢,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 病原性大腸菌のPersister細胞のストレス耐性とその制御に関する研究

    力武舞夢,益田時光,坂本英莉花,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • レタス低温誘導性親水性タンパク質の抗菌活性に関する研究

    新名祐作,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • レタス低温誘導性親水性タンパク質の抗菌活性に関する研究

    新名祐作,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 野菜種子およびスプラウト由来新規抗菌タンパク質の探索に関する研究

    中川義健,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 野菜種子およびスプラウト由来新規抗菌タンパク質の探索に関する研究

    中川義健,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 食中毒細菌特異的溶菌ファージの利用に関する研究

    菅本晃子,古田宗宜,奈須敬之,Hoang Minh Duc,Jaroenkolkit Pruet,廣野未来,Huang Hung Hsin,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 食中毒細菌特異的溶菌ファージの利用に関する研究

    菅本晃子,古田宗宜,奈須敬之,Hoang Minh Duc,Jaroenkolkit Pruet,廣野未来,Huang Hung Hsin,益田時光,本城賢一,宮本敬久

    (公社)日本食品科学工学会第68回大会  2021.8 

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    Event date: 2021.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Study on Bacteriophage for Controlling Campylobacter jejuni

    Su Zar Chi Lwin, Miku Hirono, Takayuki Nasu, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Study on Bacteriophage for Controlling Campylobacter jejuni

    Su Zar Chi Lwin, Miku Hirono, Takayuki Nasu, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Occurrence, antimicrobial resistance, genetic diversity of Listeria monocytogenes at fish-processing plants in Vietnam

    Phan Nguyen Trang, Yoshimitsu Masuda, Ken-ichi Honjoh, Tong Thi Anh Ngoc, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Occurrence, antimicrobial resistance, genetic diversity of Listeria monocytogenes at fish-processing plants in Vietnam

    Phan Nguyen Trang, Yoshimitsu Masuda, Ken-ichi Honjoh, Tong Thi Anh Ngoc, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Application of the bacteriophage cocktails to control Clostridium perfringens in food

    Tahir Noor Mohammadi, Yuncheng Li, Cunkuan Shen, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2021.8 

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    Event date: 2021.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Characterization and utilization of phages specific to campylobacter coli International conference

    Hung-Hsin Huang, Yu Zhang, Nanami Asoshima, Hoang Minh Duc, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh & Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • A novel bacteriophage endolysin Lysstg2 with high antibacterial and antibiofilm activity against Salmonella Typhimurium and Pseudomonas on various food and food contact surfaces International conference

    Yu Zhang, Hung-Hsin Huang, Hoang Minh Duc, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Screening of plant and aquatic organism extracts with antimicrobial activities against foodborne pathogens in Japan International conference

    Cunkuan Shen, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Prevalence and characterization of Listeria monocytogenes in fish and fish-processing environment in Vietnam International conference

    Trang Phan Nguyen, Yoshimitsu Masuda, Ken-ichi Honjoh1, Anh Ngoc Tong Thi, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Effectiveness of isolated and characterized phages in reducing Clostridium perfringens in food International conference

    Tahir Noor Mohammadi, Cunkuan Shen, Yuncheng Li, Mahmoud Gamaleldin Zayda, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Genetic characterization of Campylobacter jejuni phages and biocontrol of C. jejuni using the phages and EDTA International conference

    Su Zar Chi Lwin, Miku Hirono, Takayuki Nasu,YoshimitsuMasuda, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Effect of myricetin on the phage susceptibility and gene expression of Escherichia coli International conference

    Phyo Htet Htet Kyaw, Tomoka Murayama, Shota Tanaka, Koshiro Futada, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    WORLD MICROBE FORUM  2021.6 

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    Event date: 2021.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • 食材の低温貯蔵が志賀毒素産生大腸菌の耐熱性に及ぼす影響

    仲原裕貴、益田時光、本城賢一、宮本敬久

    令和2 年度 (公社) 日本食品科学工学会 西日本支部大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学西新プラザ   Country:Japan  

  • サルモネラのバイオフィルム形成に与えるアミノ酸の影響

    久保凜佳、沈 存寛、益田時光、本城賢一、宮本敬久

    令和2 年度 (公社) 日本食品科学工学会 西日本支部大会  2020.11 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学西新プラザ   Country:Japan  

  • Effects of Bacteriophage on Inhibition and Removal of Multispecies Biofilms of Escherichia coli O157 and Non-O157 International conference

    Yu Zhang, Minh Duc Hoang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    ASM Microbe 2020  2020.6 

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    Event date: 2020.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Anti-biofilm activities of naturally occurring peptides against foodborne pathogens International conference

    Cunkuan Shen, Rinka Kubo, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    ASM Microbe 2020  2020.6 

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    Event date: 2020.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Mechanism for antibacterial action of epigallocatechin gallate and theaflavin-3,3´-digallate on Clostridium perfringens International conference

    2020.6 

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    Event date: 2020.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:United States  

  • Characterization and Utilization of Phages Specific to Campylobacter Coli International conference

    Hung-Hsin Huang, Nanami Asoshima, Hoang Min Duc, Jun Sato, Yu Zhang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    ASM Microbe 2020  2020.6 

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    Event date: 2020.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • 大腸菌におけるバクテリオファージ耐性化機構の解明

    二田昂志郎、田中翔大、益田 時光、本城 賢一、宮本 敬久

    日本農芸化学会2019年度西日本・中四国支部合同沖縄大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄県青年会館(那覇市)、琉球大学(沖縄県中頭郡)   Country:Japan  

  • ガラクチノール合成酵素遺伝子高発現によるレタスの凍結耐性向上に関する研究

    木村太喜、黒川将、益田 時光、本城 賢一、宮本 敬久

    日本農芸化学会2019年度西日本・中四国支部合同沖縄大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄県青年会館(那覇市)、琉球大学(沖縄県中頭郡)   Country:Japan  

  • Microbiological assessment of Pangasianodon hypophthalmus fish during processing chain in Mekong Delta, Vietnam International conference

    Phan Nguyen Trang, Tong Thi Anh Ngoc, Yoshimitsu Masuda, Ken-ichi Honjoh, and Takahisa Miyamoto

    AFELiSA 2019 International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (The 16th International Joint Symposium between Korea and Japan)  2019.11 

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    Event date: 2019.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Antimicrobial Resistance of Campylobacter from meat International conference

    2019.11 

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    Event date: 2019.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:米子コンベンションセンター BIG SHIP(米子市)   Country:Japan  

  • Transcriptional response of Salmonella Typhimurium to e-polylysine during initial attachment on petri-dish

    日本防菌防黴学会第46回年次大会  2019.9 

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    Event date: 2019.9

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:千里ライフサイエンスセンター(大阪府豊中市)   Country:Japan  

  • 溶菌ファージおよび食品添加物によるCampylobacterの制御

    廣野未来、古田宗宜、奈須敬之、Hoang Minh Duc、Jaroenkolkit Pruet、益田時光、本城賢一、宮本敬久

    日本防菌防黴学会第46回年次大会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:千里ライフサイエンスセンター(大阪府豊中市)   Country:Japan  

  • オキシテトラサイクリン曝露による土壌中の薬剤耐性菌発現に関する研究

    香坂拓哉、益田時光、木嶋伸行、石井孝昭、本城賢一、宮本敬久

    公益社団法人 日本食品科学工学会 第66回大会  2019.8 

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    Event date: 2019.8

    Language:Japanese  

    Venue:藤女子大学北16条キャンパス(札幌市)   Country:Japan  

  • Clostridium perfringensに対するカテキンおよびテアフラビンと食品添加物の抗菌作用

    緒方はるか、Noor Mohammadi Tahir、佐藤惇、園田拓三、益田時光、本城賢一、宮本敬久

    公益社団法人 日本食品科学工学会 第66回大会  2019.8 

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    Event date: 2019.8

    Language:Japanese  

    Venue:藤女子大学北16条キャンパス(札幌市)   Country:Japan  

  • 植物ポリフェノールによる腸管出血性大腸菌の病原性抑制機構

    坂上和、中道璃名子、篠原由依、佐藤惇、園田拓三、益田時光、本城賢一、宮本敬久

    公益社団法人 日本食品科学工学会 第66回大会  2019.8 

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    Event date: 2019.8

    Language:Japanese  

    Venue:藤女子大学北16条キャンパス(札幌市)   Country:Japan  

  • サルモネラおよびリステリアに対するポリフェノール製剤と食品添加物の併用効果

    李云程、守田美保、呉藤伊織、佐藤惇、園田拓三、益田時光、本城賢一、宮本敬久

    公益社団法人 日本食品科学工学会 第66回大会  2019.8 

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    Event date: 2019.8

    Language:Japanese  

    Venue:藤女子大学北16条キャンパス(札幌市)   Country:Japan  

  • Antimicrobial Resistance of Campylobacter from Chicken International conference

    Khin Zar Linn, Yoshimitsu Masuda, Ken-ichi Honjoh, and Takahisa Miyamoto

    2019.8 

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    Event date: 2019.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Antimicrobial Resistance of Escherichia coli Isolated from Clinical and Food Sources in Japan, Fukuoka International conference

    Aung Ko Ko Minn,Yoshimitsu Masuda, Ken-ichi Honjoh, and Takahisa Miyamoto

    2019.8 

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    Event date: 2019.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Antimicrobial resistance of Pathogenic E.coli from clinical and food sources in Fukuoka, Japan

    Aung Ko Ko Minn, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Evaluation of Coliform and Enterobacteriaceae as Indicator Bacteria in Foods

    Muhammad Iqbal Haris, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • Biocontrol of Staphylococcus aureus planktonic and biofilm cells using lytic bacteriophage International conference

    Minh Duc Hoang, Minh Son Hoang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    FEMS 2019 8th Congress of European Microbiologists  2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • Effect of epigallocatechin gallate on gene expression of Staphylococcus aureus International conference

    Apisada Kitichalermkiat, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    FEMS 2019 8th Congress of European Microbiologists  2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • Effects of baicalein on the cytotoxicity, production and secretion of Shiga toxins of enterohaemorrhagic Escherichia coli International conference

    Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Motokazu Naykayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    FEMS 2019 8th Congress of European Microbiologists  2019.7 

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • Virulence and molecular typing of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian cheese samples. International conference

    M. Zayda, A. Elbagory, K. Honjoh, A. Hammad, Y. Masuda, and T. Miyamoto

    2019 ADSA Annual Meeting  2019.6 

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    Event date: 2019.6

    Language:English  

    Country:United States  

  • Effects of polyphenols against Staphylococcal enterotoxin production International conference

    Apisada Kitichalermkiat, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    The 21st FOOD INNOVATION ASIA CONFERENCE 2019  2019.6 

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    Event date: 2019.6

    Language:English  

    Country:Thailand  

  • 大腸菌を用いたレタス由来ガラクチノール合成酵素遺伝子の機能解析

    木村太喜、益田 時光、本城 賢一、宮本 敬久

    日本農芸化学会2019年度大会  2019.3 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農業大学世田谷キャンパス(東京都世田谷区)   Country:Japan  

  • 腸管出血性大腸菌の病原性に対するLuteolinの影響

    中道璃名子、篠原由衣、益田時光、本城賢一、宮本敬久

    日本食品衛生学会第114回学術講演会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:広島国際会議場(広島市)   Country:Japan  

  • 大腸菌の耐熱性におけるTAシステムの機能解明

    坂本英莉花、益田時光、本城賢一、宮本敬久

    日本食品衛生学会第114回学術講演会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:広島国際会議場(広島市)   Country:Japan  

  • 大腸菌におけるバクテリオファージ耐性化機構の解明

    二田昂志郎、田中翔大、益田時光、本城賢一、宮本敬久

    日本防菌防黴学会第45回年次大会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:タワーホール船堀(東京都)   Country:Japan  

  • 細菌のバイオフィルム形成に関与するTAシステムの解明

    王婧、益田時光、本城賢一、宮本敬久

    日本防菌防黴学会第45回年次大会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:タワーホール船堀(東京都)   Country:Japan  

  • テアフラビン類の抗菌作用機構に関する研究

    守田美保、呉藤伊織、上山雄基、佐藤惇、園田拓三、益田時光、本城賢一、宮本敬久

    第23回日本フードファクター学会学術集会、第12回日本ポリフェノール学会学術集会、第15回日本カテキン学会年次学術集会合同学術集会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:メルパルク京都(京都市)   Country:Japan  

  • 植物ポリフェノールの腸管出血性大腸菌の病原性抑制効果

    篠原由衣、山田彬史、佐藤 惇、園田拓三、益田時光、本城賢一、宮本敬久

    第23回日本フードファクター学会学術集会、第12回日本ポリフェノール学会学術集会、第15回日本カテキン学会年次学術集会合同学術集会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:メルパルク京都(京都市)   Country:Japan  

  • Mechanism for improvement of thermal resistance of Salmonella under high sucrose concentration International conference

    Guo Y., Ou L., Masuda Y., Honjoh K., Miyamoto T.

    26th International ICFMH Conference - FoodMicro 2018  2018.9 

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    Event date: 2018.9

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Germany  

  • Effects of salts on heat resistance and recovery from injury of Salmonella International conference

    Cui X., Hu C., Masuda Y., Honjoh K., Miyamoto T.

    26th International ICFMH Conference - FoodMicro 2018  2018.9 

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    Event date: 2018.9

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Germany  

  • Antimicrobial Resistance of Pathogenic E. coli from clinical and food sources in Fukuoka, Japan International conference

    Aung Ko Ko Minn,Yoshimitsu Masuda, Ken-ichi Honjoh, and Takahisa Miyamoto

    5th JASID Western Japan Research Meeting  2018.8 

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    Event date: 2018.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Antimicrobial Resistance Profiles of Listeria monocytogenes Isolated from Chicken Meats in Fukuoka, Japan International conference

    Aye Thida Maung, Yoshimitsu Masuda, Ken-ichi Honjoh, and Takahisa Miyamoto

    5th JASID Western Japan Research Meeting  2018.8 

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    Event date: 2018.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 大腸菌を用いたレタス由来ガラクチノール合成酵素遺伝子の機能解析

    #木村太喜, #黒川将, @益田時光, @本城賢一, @宮本敬久

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場(北九州市)   Country:Japan  

  • ハツカダイコン栽培段階における Listeria monocytogenesの汚染機構解明と収穫後の菌の消長について

    #李 重延, #須田千尋, @益田時光, @本城賢一, @宮本敬久

    第55回化学関連支部合同九州大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場(北九州市)   Country:Japan  

  • Mechanism for antibacterial action of epigallocatechin gallate on Clostridium perfringens

    Tahir Noor Mohammadi, Jun Sato, Ayumi Tomita, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2017.11 

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    Event date: 2017.11

    Language:English  

    Country:Japan  

  • Antimicrobial resistance of Listeria monocytogenes isolated from chicken meats in Fukuoka, Japan

    Aye Thida Maung, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto

    2017.11 

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    Event date: 2017.11

    Language:English  

    Country:Japan  

  • 植物ポリフェノールのベロ毒素活性阻害効果

    篠原由衣, 山田彬史, 益田 時光, 本城 賢一, 宮本 敬久

    第38回日本食品微生物学会学術総会  2017.10 

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    Event date: 2017.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:徳島県郷土文化会館(徳島市)   Country:Japan  

  • バクテリオファージによる生肉の腸管出血性大腸菌制御

    田中 翔大, Hoang Minh Son, 益田 時光, 本城 賢一, 宮本 敬久

    日本防菌防黴学会第44回年次大会  2017.9 

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    Event date: 2017.9

    Language:Japanese  

    Venue:千里ライフサイエンスセンター(大阪府豊中市)   Country:Japan  

  • NaClによるサルモネラの耐熱性向上機構の解明

    胡 伝琪, 郭 越, 崔 ?文, 欧 柳舒, 磯脇 千佳, 倉光 結実子, 益田 時光, 本城 賢一, 宮本 敬久

    日本防菌防黴学会第44回年次大会  2017.9 

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    Event date: 2017.9

    Language:Japanese  

    Venue:千里ライフサイエンスセンター(大阪府豊中市)   Country:Japan  

  • Revalence of antimicrobial resistance and plasmid-mediated quinolone resistance gene in Salmonella isolates from chicken meat International conference

    2017.8 

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    Event date: 2017.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Antimicrobial Resistance of Listeria monocytogenes Isolated from Chicken Meats in Fukuoka, Japan International conference

    2017.8 

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    Event date: 2017.8

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ハツカダイコン栽培段階におけるリステリアの可食部汚染機構の解明

    本城 賢一, 須田 千尋, 城 聖実, 李 重延, 木嶋 伸行, 前田 征之, 益田 時光, 宮本 敬久

    公益社団法人 日本食品科学工学会 第64回大会  2017.8 

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    Event date: 2017.8 - 2018.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本大学湘南キャンパス(藤沢市)   Country:Japan  

  • 植物ポリフェノールによる腸管出血性大腸菌の病原性抑制 International conference

    篠原由衣, 山田彬史, 益田 時光, 本城 賢一, 宮本 敬久

    第54回化学関連支部合同九州大会 外国人研究者交流国際シンポジウム  2017.7 

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    Event date: 2017.7

    Language:Japanese  

    Venue:北九州国際会議場(北九州市)   Country:Japan  

  • Prevalence and antimicrobial resistance and plasmid-mediated quinolone resistance gene in Salmonella isolates from chicken International conference

    2017.1 

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    Event date: 2017.7

    Language:English  

    Country:Japan  

  • 食中添加物によるサルモネラのバイオフィルム形成阻害機構の解明

    立石 実麗, Md Tariqul Islam, 小椋 彩, 森永 憲子, 益田 時光, 本城 賢一, 宮本 敬久

    日本農芸化学会2017年度大会  2017.3 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ウェスティン都ホテル京都/京都女子大学(京都府京都市)   Country:Japan  

  • 大腸菌の熱耐性におけるTAシステムの機能の解明

    益田 時光, Hoang Minh Duc, 二石 彩映, 中山 文, 本城 賢一, 宮本 敬久

    日本農芸化学会2017年度大会  2017.3 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ウェスティン都ホテル京都/京都女子大学(京都府京都市)   Country:Japan  

  • サルモネラの耐熱性に及ぼす生育培地のNaCl濃度の影響

    宮本 敬久, 欧 柳舒, 郭 越, 磯脇 千佳, 倉光 結実子, 崔 暁文, 胡 伝琪, 益田 時光, 本城 賢一

    第112回日本食品衛生学会学術講演会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:函館国際ホテル(北海道函館市)   Country:Japan  

  • 大腸菌の熱耐性、損傷菌発生におけるTAシステムの機能の解明

    益田 時光, Hoang Minh Duc, 二石 彩映, 中山 文, 本城 賢一, 宮本 敬久

    第112回日本食品衛生学会学術講演会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:函館国際ホテル(北海道函館市)   Country:Japan  

  • 低水分活性食品におけるサルモネラの損傷および回復機構の解明

    郭 越, 欧 柳舒, 磯脇 千佳, 倉光 結実子, 益田 時光, 本城 賢一, 宮本 敬久

    日本防菌防黴学会第43回年次大会  2016.9 

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    Event date: 2016.9

    Language:Japanese  

    Venue:きゅりあん(東京都品川区)   Country:Japan  

  • ベロ毒素活性阻害および細胞保護作用を示すポリフェノール

    山田 彬史, 益田 時光, 本城 賢一, 宮本 敬久

    日本防菌防黴学会第43回年次大会  2016.9 

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    Event date: 2016.9

    Language:Japanese  

    Venue:きゅりあん(東京都品川区)   Country:Japan  

  • 低水分活性条件におけるサルモネラの熱耐性能の向上と加熱損傷及び回復機構の解明

    益田 時光, 倉光 結実子, 欧 柳舒, 磯脇 千佳, 本城 賢一, 宮本 敬久

    日本防菌防黴学会第43回年次大会  2016.9 

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    Event date: 2016.9

    Language:Japanese  

    Venue:きゅりあん(東京都品川区)   Country:Japan  

  • レタス (Lactuca sativa. L)由来の低温誘導性galactinol 合成酵素遺伝子のクローニング

    黒川 将, 岡野 仁美, 川畑 彩, 益田 時光, 本城 賢一, 宮本 敬久

    第34回日本植物細胞分子生物学会(上田)大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:信州大学繊維学部(上田市)   Country:Japan  

  • 低水分活性条件でのサルモネラの加熱損傷および回復機構の解明

    益田 時光, 欧 柳舒, 磯脇 千佳, 本城 賢一, 宮本 敬久

    公益社団法人 日本食品科学工学会 第63回大会  2016.8 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学天白キャンパス(名古屋市)   Country:Japan  

  • 生肉より分離した腸管出血性大腸菌特異的溶菌バクテリオファージの性状

    宮本 敬久, ホン ミン ソン, ホン ミン ドク, 益田 時光, 本城 賢一

    公益社団法人 日本食品科学工学会 第63回大会  2016.8 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名城大学天白キャンパス(名古屋市)   Country:Japan  

  • Leaderless Leading: Introducing post-translational modifications into leaderless bacteriocins Invited

    BMB2015 

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    Event date: 2015.12

    Language:Japanese  

    Country:Japan  

  • Leaderless leading: introducing post-translational modifications into leaderless bacteriocins. International conference

    Yoshimitsu Masuda, Andrius Buivydas, Oscar P Kuipers

    11th International Symposium on Lactic Acid Bacteria 

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    Event date: 2014.8 - 2014.9

    Language:English  

    Country:Netherlands  

  • Leaderless leading: introducing post-translational modifications into leaderless bacteriocins. International conference

    Yoshimitsu Masuda, Andrius Buivydas, Oscar P Kuipers

    4th International Antimicrobial peptides symposium  2014.6 

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    Event date: 2014.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:France  

  • Lanthipeptide production using Zink regulated EXpression (ZIREX). International conference

    Yoshimitsu Masuda, Dongdong Mu, Manuel Montalban-Lopez, Oscar P Kuipers

    2013.6 

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    Event date: 2013.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Italy  

  • Immunity mechanism and nutrition-adaptive regulation system of weissellicins Y and M, two novel leaderless bacteriocins from Weissella hellenica QU 13 International conference

    Yoshimitsu Masuda, Takeshi Zendo, Rodney Perez H, Juan Jose Jimenes, Pablo E Hernandez, Carmen Heranz, Luis M Cintas, Kenji Sonomoto

    A Biochemical Society Focused Meeting: How bugs kill bugs? 

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    Event date: 2012.7

    Language:English  

    Country:United Kingdom  

  • Biosynthesis machinery and multiple bacteriocin production system of weissellicin YM, novel leaderless bacteriocins International conference

    Yoshimitsu Masuda, Takeshi Zendo, Rodney Perez H, Juan Jose Jimenes, Carmen Heranz, Luis M Cintas, Pablo E Hernandez, Kenji Sonomoto

    10th International Symposium on Lactic Acid Bacteria 

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    Event date: 2011.8 - 2011.9

    Language:English  

    Country:Netherlands  

  • Characterization and structure analysis of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13 International conference

    2nd International Antimicrobial peptides symposium  2009.6 

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    Event date: 2009.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:France  

  • Screening of novel bacteriocins from newly isolated lactic acid bacteria. International conference

    Multi-Functionality and Sustainability of Land Use in South East and East Asia  2007.9 

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    Event date: 2007.9

    Language:English  

    Country:Japan  

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MISC

  • 食中毒細菌Persisterの重要性、ストレス耐性とその制御について Reviewed

    @益田時光

    日本食品科学工学学会誌   2023.7

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    Repository Public URL: https://hdl.handle.net/2324/7236463

  • 細菌のPersister細胞の耐熱性とインドールの効果 Reviewed

    益田時光

    バイオサイエンスとインダストリー   2021.9

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  • 食中毒細菌Persisterの重要性,ストレス耐性とその制御について

    益田 時光

    日本食品科学工学会誌   70 ( 8 )   311 - 318   2023.8   ISSN:1341-027X

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    Language:Japanese   Publisher:(公社)日本食品科学工学会  

    Persisterは、自身の代謝や細胞活性を低下させ休眠状態のようになることで抗生物質などのストレスに耐性を示す細菌細胞であり、遺伝子変異による耐性菌と違って抗生物質存在下では増殖することはない。今回、Persister形成機構と、Persister形成を直接的に制御することが報告されている「Toxin-antitoxinシステム」、Persisterの制御方法、Persisterのストレス耐性について概説し、臨床におけるPersisterの重要性について述べた。

  • 細菌のPersisterの特性とその制御 ResistanceとPersistenceは似て非なるもの

    益田 時光

    化学と生物   60 ( 5 )   232 - 239   2022.5   ISSN:0453-073X

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  • New type non-lantibiotic bacteriocins: Circular bacteriocins and leaderless bacteriocins

    Yoshimitsu Masuda, Takeshi Zendo, Kenji Sonomoto

    Beneficial Microbes   2012.3

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    Repository Public URL: https://hdl.handle.net/2324/7236464

  • Nisin-an antibacterial peptide with exceptional potential-

    Yoshimitsu Masuda, Takeshi Zendo, Kenji Sonomoto

    Milk Science   2010.6

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Professional Memberships

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry

  • Japanese Society for Food Science and Technology

  • 日本食品衛生学会

  • 日本食品衛生学会

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  • Japanese Society for Food Science and Technology

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  • Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Research Projects

  • Functional analysis of cold-inducible VPE gene and development of freeze-resistant lettuce based on the analysis

    Grant number:24K08764  2024.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

    CiNii Research

  • Construction a scientific basis for the use of phages to ensure food safety

    Grant number:23K26855  2024.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant type:Scientific research funding

    CiNii Research

  • 有機酸による食中毒細菌Persisterの制御

    2024

    食生活研究会研究助成金

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    Authorship:Principal investigator  Grant type:Contract research

  • Establsihment of a scientific foundation for the use of phages in ensuring food safety

    Grant number:23H02162  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant type:Scientific research funding

    CiNii Research

  • 食の安全確保におけるファージ利用の科学的基盤構築

    Grant number:23729786  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ゲノム編集によるバクテリオファージ特性の改変とLLBファージの応用に関する研究

    2022.4 - 2025.3

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    Authorship:Principal investigator 

  • ゲノム編集によるバクテリオファージ特性の改変とLLBファージの応用に関する研究

    Grant number:22K05384  2022 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    益田 時光, 本城 賢一, 宮本 敬久

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    Authorship:Principal investigator  Grant type:Scientific research funding

    本研究では、食中毒細菌の制御を目的として、CRISPR -Casシステムに代表されるゲノム編集技術を用いたバクテリオファージ(以降ファージ)遺伝子改変技術の基盤の構築と改変ファージの応用を目指す。特に、ファージの宿主特異性を担うテイルファイバーについての改変や、宿主菌のゲノム中にファージゲノム情報が入り込んでしまう溶原化に関連する遺伝子を特定、取り除くことで安全性の高い溶菌ファージに変換することなどを目指す。また、宿主菌のファージ耐性化を防ぐための抗菌力強化のために、3)抗菌ペプチドリーダーレスバクテリオシン(LLB)産生ファージ(以降LLBファージ)の作製とその応用についての検討を行う。

    CiNii Research

  • 「ファージが守る食の安全」に寄与する学術基盤の構築

    Grant number:19H02912  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    宮本 敬久, 本城 賢一, 益田 時光

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    本研究では、食中毒細菌によるリスク低減のため、バクテリオファージ(以降ファージ)およびファージの効果を促進させる安全性の高い天然成分を併用した効果的な食中毒細菌制御に寄与する学術的基盤を確立する。このため、腸管出血性大腸菌、サルモネラ、カンピロバクター、黄色ブドウ球菌、リステリアに対する溶菌ファージを単離してその性質を明らかにする。また、細菌の持つ免疫システム以外の溶菌ファージに対する耐性化に関与するシステムを解明し、ファージ耐性化を阻害する天然成分を植物抽出物などから見出してファージとの併用効果のメカニズムを解明する。

    CiNii Research

  • LLBファージを用いた新たな食中毒細菌制御法の確立

    2018.4 - 2022.3

    九州大学大学院農学研究院 

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    Authorship:Principal investigator 

    細菌特異的なファージとリーダーレスバクテリオシン(LLB)をCRISPR-Casなどの遺伝学的技術を用いて融合し、LLBファージを構築し食中毒細菌の制御を目指す。

  • LLBファージという新たな食中毒細菌制御法の確立

    Grant number:18K14378  2018 - 2021

    日本学術振興会  科学研究費助成事業  若手研究

    益田 時光

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    Authorship:Principal investigator  Grant type:Scientific research funding

    本研究は、リーダレスバクテリオシン(LLB)を産生するバクテリオファージ(ファージ)、LLBファージを構築し、食中毒細菌の制御における新しい手法を創出することを目的とした。また、ファージの遺伝子改変のための新たなプラットフォームの構築についても検討を行った。
    その結果、大腸菌を標的とするlnqQ-T7、大腸菌O157H7株を標的とする5種類のLLB-ECP52、多剤耐性黄色ブドウ球菌(MRSA)を標的としたlnqQ-vPSARaといったLLBファージの作成に成功した。
    さらに、大腸菌や黄色ブドウ球菌内におけるCRISPR-Casを利用した溶原ファージゲノム編集システムの構築にも成功した。

    CiNii Research

  • 細菌の熱耐性におけるトキシンアンチトキシンシステムの役割

    2017.4 - 2019.3

    九州大学大学院農学研究院 

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Class subject

  • 食品衛生化学実験

    2021.10 - 2022.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2021.10 - 2022.3   Second semester

  • 食品衛生化学実験

    2020.10 - 2021.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2020.10 - 2021.3   Second semester

  • 食品衛生化学実験

    2019.10 - 2020.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2019.10 - 2020.3   Second semester

  • 食品衛生化学実験

    2019.10 - 2020.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2019.10 - 2020.3   Second semester

  • 自然科学総合実験

    2019.4 - 2019.9   First semester

  • 食品衛生化学実験

    2018.10 - 2019.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2018.10 - 2019.3   Second semester

  • 食料化学工学プロジェクト演習

    2018.4 - 2019.3   Full year

  • 自然科学総合実験

    2018.4 - 2019.3   Full year

  • 自然科学総合実験(発展)

    2017.12 - 2018.2   Winter quarter

  • 食品衛生化学実験

    2017.10 - 2018.3   Second semester

  • 分析化学実験(食糧化学工学分野)

    2017.10 - 2018.3   Second semester

  • 自然科学総合実験

    2017.4 - 2018.3   Full year

  • 食料化学工学プロジェクト演習

    2017.4 - 2018.3   Full year

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Outline of Social Contribution and International Cooperation activities

  • Open campus for public and the lecture for exchange foreign students.

Travel Abroad

  • 2018.9 - 2019.9

    Staying countory name 1:Australia   Staying institution name 1:University of Queensland

  • 2017.3

    Staying countory name 1:Australia   Staying institution name 1:University of New South Wales

  • 2014.4 - 2016.2

    Staying countory name 1:Netherlands   Staying institution name 1:Molecular Genetics, Department of Genetics, GBB, University of Groningen

  • 2012.12 - 2014.1

    Staying countory name 1:Netherlands   Staying institution name 1:Molecular Genetics, Department of Genetics, GBB, University of Groningen

  • 2012.10

    Staying countory name 1:Thailand   Staying institution name 1:BIOTEC, National Science and Technology Development Agency (NASTDA)

  • 2012.5 - 2012.8

    Staying countory name 1:Netherlands   Staying institution name 1:Molecular Genetics, Department of Genetics, GBB, University of Groningen

  • 2009.5 - 2009.8

    Staying countory name 1:Spain   Staying institution name 1:Universidad Complutense de Madrid

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