Updated on 2024/09/30

Information

 

写真a

 
ANAI TOYOAKI
 
Organization
Faculty of Agriculture Department of Bioresource Sciences(University Farm) Professor
School of Agriculture University Farm(Joint Appointment)
School of Agriculture Department of Bioresource and Bioenvironment(Joint Appointment)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioresource Sciences(Joint Appointment)
School of Education (Joint Appointment)
Title
Professor
Contact information
メールアドレス
Tel
0928024536
Profile
1. Screening for novel valuable mutant genes from the soybean high-density mutant population, and characterization of their molecular mechanisms 2. Improvement of soybean seed qualities (the content of lipids, carbohydrates, isoflavones, etc.) using the mutant genes 3. Improvement of soybean agronomic traits (grass shape, stress tolerance, light responsibility, plant-microbe interaction, etc.) using the mutant genes

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

  • Environmental Science/Agriculture Science / Crop production science

Degree

  • Ph. D., Hokkaido Univ.

Research History

  • Kyushu University Faculty of Agriculture Professor Ph. D.

    2021.6 - Present

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  • 筑波大学、佐賀大学

Research Interests・Research Keywords

  • Research theme:突然変異体

    Keyword:突然変異体

    Research period: 2024

  • Research theme:突然変異

    Keyword:突然変異

    Research period: 2024

  • Research theme:育種学

    Keyword:育種学

    Research period: 2024

  • Research theme:遺伝子破壊

    Keyword:遺伝子破壊

    Research period: 2024

  • Research theme:遺伝子発現

    Keyword:遺伝子発現

    Research period: 2024

  • Research theme:遺伝子

    Keyword:遺伝子

    Research period: 2024

  • Research theme:逆遺伝学

    Keyword:逆遺伝学

    Research period: 2024

  • Research theme:脂肪酸不飽和化酵素

    Keyword:脂肪酸不飽和化酵素

    Research period: 2024

  • Research theme:脂肪酸

    Keyword:脂肪酸

    Research period: 2024

  • Research theme:Development of novel mutant genes for improvement of seed quality and the other agronomic traits

    Keyword:soybean, mutation breeding, seed quality, agronomic traits

    Research period: 2021.6

Awards

  • 日本育種学会功労賞

    2021.3   日本育種学会  

  • 日本育種学会賞

    2020.3   日本育種学会  

  • 日本育種学会論文賞

    2011.4   日本育種学会  

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    Novel GmFAD2-1b mutant alleles created by reverse genetics induce drastic elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles

Papers

  • Mutations in the genes responsible for the synthesis of furan fatty acids resolve the light-induced off-odor in soybean oil. Reviewed International journal

    S. Watanabe, A. Omagari, R. Yamada, A. Matsumoto, Y. Kimura N. Makita, E. Hiyama, Y. Okamoto, R. Okabe, T. Sano, T. Sato, M. Suzuki, S. Saito, T. Anai

    The Plant Journal   117   1239 - 1249   2023.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1111/tpj.16560

  • <i>In Situ</i> Raman Hyperspectral Analysis of Microbial Colonies for Secondary Metabolites Screening

    Suwa, S; Ando, M; Nakashima, T; Horii, S; Anai, T; Takeyama, H

    ANALYTICAL CHEMISTRY   96 ( 37 )   14909 - 14917   2024.8   ISSN:0003-2700 eISSN:1520-6882

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    Language:English   Publisher:Analytical Chemistry  

    Since the discovery of penicillin, a vast array of microbial antibiotics has been identified and applied in the medical field. Globally, the search for drug candidates via microbial screening is ongoing. Traditional screening methods, however, are time-consuming and require labor-intensive sample processing, significantly reducing throughput. This research introduces a Raman spectroscopy-based screening system tailored to the in situ analysis of microbial colonies on solid culture media. Employing multivariate curve resolution-alternating least-squares (MCR-ALS) for spectral decomposition, our approach reveals the production of secondary metabolites at the single colony level. We enhanced the microbial culture method, enabling direct, high signal-to-noise (S/N) ratio Raman spectroscopic measurements of colonies of Escherichia coli and actinomycetes species. Through semisupervised MCR analysis using the known spectra of actinorhodin and undecylprodigiosin as references, we accurately assessed the production of these compounds by Streptomyces coelicolor A3(2). Furthermore, we herein successfully detected the production of amphotericin B by Streptomyces nodosus, even in the absence of prior spectral information. This demonstrates the potential of our technique in the discovery of secondary metabolites. In addition to enabling the detection of the above-mentioned compounds, this analysis revealed the heterogeneity of the spatial distribution of their production in each colony. Our technique makes a significant contribution to the advancement of microbial screening, offering a rapid, efficient alternative to conventional methods and opening avenues for secondary metabolites discovery.

    DOI: 10.1021/acs.analchem.4c02906

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  • Analysis of microbial dynamics in the soybean root-associated environments from community to single-cell levels Reviewed International journal

    Masako Kifushi, Yohei Nishikawa, Masahito Hosokawa, Keigo Ide, Masato Kogawa, Toyoaki Anai, Haruko Takeyama

    Journal of Bioscience and Bioengineering   137 ( 6 )   429 - 436   2024.6   ISSN:1389-1723 eISSN:1347-4421

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jbiosc.2024.02.007

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  • The major soybean off-flavor production from certain fatty acids and their genetical improvement

    ANAI Toyoaki

    Journal of Japan Association on Odor Environment   55 ( 3 )   171 - 175   2024.5   ISSN:13482904 eISSN:13497847

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    Language:Japanese   Publisher:Japan Association on Odor Environment  

    <p>Soybean is one of the commercially important legume crops in the world, and it contains about 40% protein and 20% oil by seed dry weight. However, a series of off-flavors produced by the oxidation of certain fatty acids that consist of this oil have been a problem in various soybean-based foods, including soybean oil. In this paper, we explain the mechanism of fatty acid-derived off-flavor development in soybean and introduce the recent research progress on genetical improvement of soybean fatty acid composition and its application to the reduction of these off-flavors.</p>

    DOI: 10.2171/jao.55.171

    CiNii Research

  • Effect of nitrogen fixation enhancing type <i>SEN1</i> gene on soybean growth Reviewed International journal

    Shimomura, A; Nishida, Y; Yamamoto, S; Suganuma, N; Watanabe, S; Anai, T; Arima, S; Tominaga, A; Suzuki, A

    PLANT PRODUCTION SCIENCE   27 ( 2 )   137 - 149   2024.4   ISSN:1343-943X eISSN:1349-1008

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Plant Production Science  

    It is well known that the growth of legumes is greatly affected not only by fertilizer nitrogen but also by fixed nitrogen by symbiosis with rhizobia. We have reported that a polymorphism in SEN1, which is essential for nitrogen fixation in Lotus japonicus, could be used to enhance nitrogen fixation and plant growth. In this study, we examined whether the same strategy could be applied to Glycine max. Sequencing of orthologs of the LjSEN1 gene in 38 soybean cultivars revealed that the polymorphism occurred at Glyma.08g076300. We defined the standard sequence that most of the cultivars retained as Peking type SEN1, and the mutated sequence found in Enrei as Enrei type SEN1. To investigate the effect of genotype differences, we generated near-isogenic lines by crossing Enrei with Fukuyutaka, which has Peking type SEN1, and analyzed their characteristics. The results showed that Enrei type enhanced nitrogen fixation activity and promoted plant growth. Since soybean SEN1 has been reported to be a homolog of vacuolar iron transporter-like protein, we investigated the iron content in the nodules. In the result, the iron content in the nodules of Enrei type was significantly higher than that of Peking type. Iron is known to be present in the active center of nitrogenase, and the high iron content may have contributed to the increased nitrogen fixation activity. These results suggest that Enrei type SEN1 is a nitrogen fixation enhancing gene and that introduction of Enrei type SEN1 may reduce nitrogen fertilizer application and increase yield.

    DOI: 10.1080/1343943X.2024.2326643

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  • Effect of nitrogen fixation enhancing type SEN1 gene on soybean growth. Reviewed International journal

    A. Shimomura, Y. Nishida, S. Yamamoto, N. Suganuma, S. Watanabe, T. Anai, S. Arima, A. Tominaga. A. Suzuki

    Plant Production Science   27   1237 - 149   2024.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1080/1343943X.2024.2326643

  • Identification of genes responsible for the synthesis of glycitein isoflavones in soybean seeds. Invited Reviewed International journal

    M. Horitani, R. Yamada, K. Taroura, A. Maeda, T. Anai, M. Watanabe

    Plants   13   156   2024.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3390/plants13020156

  • Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds. International journal

    Naohiro Shioya, Eri Ogiso-Tanaka, Masanori Watanabe, Toyoaki Anai, Tomoki Hoshino

    Plants (Basel, Switzerland)   12 ( 16 )   2023.8   ISSN:2223-7747

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    Language:English   Publishing type:Research paper (scientific journal)  

    Genome sequencing is important for discovering critical genes in crops and improving crop breeding efficiency. Generally, fresh, young leaves are used for DNA extraction from plants. However, seeds, the storage form, are more efficient because they do not require cultivation and can be ground at room temperature. Yet, only a few DNA extraction kits or methods suitable for seeds have been developed to date. In this study, we introduced an improved (IMP) Boom method that is relatively low-cost, simple to operate, and yields high-quality DNA that can withstand long-read sequencing. The method successfully extracted approximately 8 µg of DNA per gram of seed weight from soybean seeds at an average concentration of 48.3 ng/µL, approximately 40-fold higher than that extracted from seeds using a common extraction method kit. The A260/280 and A260/230 values of the DNA were 1.90 and 2.43, respectively, which exceeded the respective quality thresholds of 1.8 and 2.0. The DNA also had a DNA integrity number value (indicating the degree of DNA degradation) of 8.1, higher than that obtained using the kit and cetyltrimethylammonium bromide methods. Furthermore, the DNA showed a read length N50 of 20.96 kbp and a maximum read length of 127.8 kbp upon long-read sequencing using the Oxford Nanopore sequencer, with both values being higher than those obtained using the other methods. DNA extracted from seeds using the IMP Boom method showed an increase in the percentage of the nuclear genome with a decrease in the relative ratio of chloroplast DNA. These results suggested that the proposed IMP Boom method can extract high-quality and high-concentration DNA that can be used for long-read sequencing, which cannot be achieved from plant seeds using other conventional DNA extraction methods. The IMP Boom method could also be adapted to crop seeds other than soybeans, such as pea, okra, maize, and sunflower. This improved method is expected to improve the efficiency of various crop-breeding operations, including seed variety determination, testing of genetically modified seeds, and marker-assisted selection.

    DOI: 10.3390/plants12162971

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  • Development of a high-quality/yield long-read sequencing-adaptable DNA extraction method for crop seeds. Invited Reviewed International journal

    N. Shioya, E. Ogiso-Tanaka, M. Watanabe, T. Anai and T. Hoshino

    Plants   12   2971   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi.org/10.3390/plants12162971

  • ダイズ突然変異体リソースの整備と新規アリルの開発に関する研究 Invited

    穴井 豊昭

    育種学研究   22 ( 2 )   174 - 177   2022.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbr.20J18

  • An Agrobacterium-mediated transformation via organogenesis regeneration of a facultative CAM plant, the common ice plant Mesembryanthemum crystallinum L Reviewed

    Sakae Agarie, Makiko Umemoto, Haruki Sunagawa, Toyoaki Anai, John C. Cushman

    Plant Production Science   23 ( 3 )   343 - 349   2020.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/1343943X.2020.1730700

  • SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth Reviewed International journal

    Yuki Nishida, Reona Hiraoka, Satomi Kawano, Norio Suganuma, Shusei Sato, Satoshi Watanabe, Toyoaki Anai, Susumu Arima, Akiyoshi Tominaga, Akihiro Suzuki

    SOIL SCIENCE AND PLANT NUTRITION   66 ( 6 )   864 - 869   2020.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/00380768.2020.1834829

  • Identification of a cytochrome P450 hydroxylase, CYP81E22, as a causative gene for the high sensitivity of soybean to herbicide bentazon Invited Reviewed International journal

    Shin Kato, Yuko Yokota, Rintaro Suzuki, Yukiko Fujisawa, Takashi Sayama, Akito Kaga, Toyoaki Anai, Kunihiko Komatsu, Nobuhiko Oki, Akio Kikuchi, Masao Ishimoto

    Theoretical and Applied Genetics   133 ( 7 )   2105 - 2115   2020.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00122-020-03580-6

  • Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed Invited Reviewed International journal

    Sarkar, Md Abdur Rauf; Otsu, Wakana; Suzuki, Akihiro; Hashimoto, Fumio; Anai, Toyoaki; Watanabe, Satoshi

    BREEDING SCIENCE   70 ( 3 )   265 - 276   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.19134

  • Characterization of an intermediate filament protein from the platyhelminth, Dugesia japonica Reviewed

    Akiko Yamamoto, Ken Ichiro Matsunaga, Toyoaki Anai, Hitoshi Kawano, Toshihisa Ueda, Toshihiko Matsumoto, Shoji Ando

    Protein and Peptide Letters   27 ( 5 )   432 - 446   2020.5

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    DOI: 10.2174/0929866526666191025102902

  • Molecular characterization of high stearic acid soybean mutants and post-transcriptional control of GmSACPD genes in the mutant with a single nucleotide deletion Invited Reviewed International journal

    Tomoki Hoshino, Nobushige Iijima, Masakazu Hata, Anri Watanabe, Tamae Kawakami, Toyoaki Anai

    Plant Gene   21   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.plgene.2019.100207

  • Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed Reviewed

    Md. Abdur Rauf Sarkar, Wakana Otsu, Akihiro Suzuki, Fumio Hashimoto, Toyoaki Anai, Satoshi Watanabe

    Breeding Science   70 ( 3 )   265 - 276   2020.3

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.19134

  • Domesticating Vigna Stipulacea: A Potential Legume Crop With Broad Resistance to Biotic Stresses Reviewed International journal

    Yu Takahashi, Hiroaki Sakai, Yuki Yoshitsu, Chiaki Muto, Toyoaki Anai, Muthaiyan Pandiyan, Natesan Senthil, Norihiko Tomooka, Ken Naito

    Frontiers in Plant Science   10   1607 - 1607   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2019.01607

  • Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation Reviewed

    Md. Abdur Rauf Sarkar, Satoshi Watanabe, Akihiro Suzuki, Fumio Hashimoto, Toyoaki Anai

    Plant Biotechnology   36 ( 4 )   241 - 251   2019.12

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.5511/plantbiotechnology.19.1025a

  • Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation Invited Reviewed International journal

    Md. Abdur Rauf Sarkar, Satoshi Watanabe, Akihiro Suzuki, Fumio Hashimoto, Toyoaki Anai

    PLANT BIOTECHNOLOGY   36 ( 4 )   241 - 251   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5511/plantbiotechnology.19.1025a

  • Soybean antiviral immunity conferred by dsRNase targets the viral replication complex Reviewed

    Kazuhiro Ishibashi, Masayasu Saruta, Takehiko Shimizu, Miao Shu, Toyoaki Anai, Kunihiko Komatsu, Naohiro Yamada, Yuichi Katayose, Masayuki Ishikawa, Masao Ishimoto, Akito Kaga

    Nature Communications   10 ( 1 )   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-019-12052-5

  • High-throughput screening and characterization of a high-density soybean mutant library elucidate the biosynthesis pathway of triterpenoid saponins Reviewed

    Panneerselvam Krishnamurthy, Yukiko Fujisawa, Yuya Takahashi, Hanako Abe, Kentaro Yamane, Kyosuke Mukaiyama, Hae Reon Son, Susumu Hiraga, Akito Kaga, Toyoaki Anai, Chigen Tsukamoto, Masao Ishimoto

    Plant and Cell Physiology   60 ( 5 )   1082 - 1097   2019.5

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcz025

  • Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean Reviewed

    Kaori Hirata, Kyoko Takagi, Tetsuya Yamada, Takashi Sayama, Toyoaki Anai, Akio Kikuchi, Masao Ishimoto

    Breeding Science   69 ( 2 )   345 - 351   2019.4

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    DOI: 10.1270/jsbbs.18091

  • Identification and characterization of a major QTL underlying soybean isoflavone malonylglycitin content Reviewed

    Satoshi Watanabe, Risa Yamada, Hazuki Kanetake, Akito Kaga, Toyoaki Anai

    Breeding Science   69 ( 4 )   564 - 572   2019.2

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.19027

  • Genetic and functional characterization of Sg-4 glycosyltransferase involved in the formation of sugar chain structure at the C-3 position of soybean saponins Reviewed

    Kyoko Takagi, Ryoichi Yano, Saeko Tochigi, Yukiko Fujisawa, Hiroki Tsuchinaga, Yuya Takahashi, Yoshitake Takada, Akito Kaga, Toyoaki Anai, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto

    Phytochemistry   156   96 - 105   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.phytochem.2018.09.002

  • Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala Reviewed

    Md. Harun-Ur-Rashid, Hironori Iwasaki, Shahanaz Parveen, Shigeki Oogai, Masakazu Fukuta, Md Amzad Hossain, Toyoaki Anai, Hirosuke Oku

    Applied Biochemistry and Biotechnology   186 ( 3 )   613 - 632   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12010-018-2745-z

  • Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins Reviewed

    Ryoichi Yano, Kyoko Takagi, Saeko Tochigi, Yukiko Fujisawa, Yuhta Nomura, Hiroki Tsuchinaga, Yuya Takahashi, Yoshitake Takada, Akito Kaga, Toyoaki Anai, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto

    Plant and Cell Physiology   59 ( 4 )   792 - 805   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcy019

  • High-resolution mapping and characterization of xa42, a resistance gene against multiple xanthomonas oryzae pv. Oryzae races in rice (oryza sativa l.) Reviewed

    Constantine Busungu, Satoru Taura, Jun Ichi Sakagami, Toyoaki Anai, Katsuyuki Ichitani

    Breeding Science   68 ( 2 )   188 - 199   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.17094

  • Molecular characterization of cytosolic cysteine synthase in Mimosa pudica Reviewed

    Md Harun Ur Rashid, Hironori Iwasaki, Shigeki Oogai, Masakazu Fukuta, Shahanaz Parveen, Md Amzad Hossain, Toyoaki Anai, Hirosuke Oku

    Journal of Plant Research   131 ( 2 )   319 - 329   2018.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10265-017-0986-5

  • Chromosomal aneuploidy improves the brewing characteristics of sake yeast Reviewed

    Masafumi Kadowaki, Yuki Fujimaru, Seiga Taguchi, Jannatul Ferdouse, Kazutaka Sawada, Yuta Kimura, Yohei Terasawa, Gennaro Agrimi, Toyoaki Anai, Hideki Noguchi, Atsushi Toyod, Asao Fujiyama, Takeshi Akao, Hiroshi Kitagaki

    Applied and Environmental Microbiology   83 ( 24 )   2017.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.01620-17

  • Genetic and Chemical Analysis of Deep Purple Flower in Soybean Reviewed

    Ryoji Takahashi, Fan Yan, Shaokang Di, Yoshinori Murai, Tsukasa Iwashina, Toyoaki Anai

    Crop Science   57 ( 4 )   1893 - 1898   2017.5

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.2135/cropsci2016.08.0673

  • Identification of quantitative trait loci for owering time by a combination of restriction site–associated DNA sequencing and bulked segregant analysis in soybean Reviewed

    67 ( 3 )   277 - 285   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.17013

  • Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants Reviewed

    Takashi Sayama, Takanari Tanabata, Masayasu Saruta, Testsuya Yamada, Toyoaki Anai, Akito Kaga, Masao Ishimoto

    Breeding Science   67 ( 4 )   363 - 369   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbs.16201

  • Fatty Acid Composition and Distribution in Wild Soybean (Glycine soja) Seeds Collected in Japan Reviewed

    Chi- Do Wee, Masatsugu Hashiguchi, Toyoaki Anai, Akihiro Suzuki, Ryo Akashi

    Asian Journal of Plant Sciences   16 ( 2 )   52 - 64   2017.3

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.3923/ajps.2017.52.64

  • Metabolic switching of astringent and beneficial triterpenoid saponins in soybean is achieved by a loss-of-function mutation in cytochrome P450 72A69 Reviewed International journal

    Ryoichi Yano, Kyoko Takagi, Yoshitake Takada, Kyosuke Mukaiyama, Chigen Tsukamoto, Takashi Sayama, Akito Kaga, Toyoaki Anai, Satoru Sawai, Kiyoshi Ohyama, Kazuki Saito, Masao Ishimoto

    Plant Journal   89 ( 3 )   527 - 539   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.13403

  • 有用作物の作成

    穴井豊昭

    2017.2

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  • Metabolic switching of astringent and beneficial triterpenoid saponins in soybean is achieved by a loss-of-function mutation in cytochrome P450 72A69 Reviewed

    Ryoichi Yano, Kyoko Takagi, Yoshitake Takada, Kyosuke Mukaiyama, Chigen Tsukamoto, Takashi Sayama, Akito Kaga, Toyoaki Anai, Satoru Sawai, Kiyoshi Ohyama, Kazuki Saito, Masao Ishimoto

    PLANT JOURNAL   89 ( 3 )   527 - 539   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.13403

  • Metabolite composition of grapefruit (Citrus paradisi) grown in Japan depends on the growing environment and harvest period Reviewed

    Yuta Kimura, Mari Naeshiro, Yuri Tominaga, Toyoaki Anai, Fuminori Komai

    Horticulture Journal   86 ( 4 )   543 - 551   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2503/hortj.MI-139

  • ダイズ突然変異体リソースの開発とその活用

    穴井豊昭

    作物研究   61 ( 61 )   67‐72   2016.11

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    Language:Japanese  

  • New Allelic Variant Discovered at Soybean Flower Color Locus W1 Encoding Flavonoid 3′5′-hydroxylase. Reviewed

    56 ( 4 )   1506 - 1513   2016.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2135/cropsci2015.07.0413

  • Mutant-based reverse genetics for functional genomics of non-model crops

    Toyoaki Anai

    Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools   1   473 - 487   2016.1

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    DOI: 10.1007/978-3-319-22521-0_16

  • GmCOL1a and GmCOL1b Function as Flowering Repressors in Soybean Under Long-Day Conditions Reviewed

    Dong Cao, Ying Li, Sijia Lu, Jialin Wang, Haiyang Nan, Xiaoming Li, Danning Shi, Chao Fang, Hong Zhai, Xiaohui Yuan, Toyoaki Anai, Zhengjun Xia, Baohui Liu, Fanjiang Kong

    PLANT AND CELL PHYSIOLOGY   56 ( 12 )   2409 - 2422   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcv152

  • Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing Reviewed

    Mai Tsuda, Akito Kaga, Toyoaki Anai, Takehiko Shimizu, Takashi Sayama, Kyoko Takagi, Kayo Machita, Satoshi Watanabe, Minoru Nishimura, Naohiro Yamada, Satomi Mori, Harumi Sasaki, Hiroyuki Kanamori, Yuichi Katayose, Masao Ishimoto

    BMC GENOMICS   16   1014   2015.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s12864-015-2079-y

  • Red/Far Red Light Controls Arbuscular Mycorrhizal Colonization via Jasmonic Acid and Strigolactone Signaling Reviewed

    Maki Nagata, Naoya Yamamoto, Tamaki Shigeyama, Yohei Terasawa, Toyoaki Anai, Tatsuya Sakai, Sayaka Inada, Susumu Arima, Masatsugu Hashiguchi, Ryo Akashi, Hideyuki Nakayama, Daisuke Ueno, Ann M. Hirsch, Akihiro Suzuki

    PLANT AND CELL PHYSIOLOGY   56 ( 11 )   2100 - 2109   2015.11

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    DOI: 10.1093/pcp/pcv135

  • A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil Reviewed

    64 ( 4 )   371 - 377   2014.12

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    DOI: 10.1270/jsbbs.64.371

  • Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2 Reviewed

    Fan Yan, Shaokang Di, Felipe Rojas Rodas, Tito Rodriguez Torrico, Yoshinori Murai, Tsukasa Iwashina, Toyoaki Anai, Ryoji Takahashi

    BMC PLANT BIOLOGY   14   58   2014.3

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    DOI: 10.1186/1471-2229-14-58

  • Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum Reviewed

    Yohei Terasawa, Kanenori Takata, Toyoaki Anai, Tatsuya M. Ikeda

    GENETICA   141 ( 7-9 )   359 - 368   2013.9

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    DOI: 10.1007/s10709-013-9735-4

  • 高等植物への菌根菌感染は光の質と量によって制御される

    鈴木 章弘, 永田 真紀, 山本 直也, 重山 珠紀, 穴井 豊昭, 有馬 進, 酒井 達也, HIRSCH Ann M

    日本作物學會紀事   82   236 - 237   2013.3

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  • Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes Reviewed

    Yasutaka Tsubokura, Hisakazu Matsumura, Meilan Xu, Baohui Liu, Hiroko Nakashima, Toyoaki Anai, Fanjiang Kong, Xiaohui Yuan, Hiroyuki Kanamori, Yuichi Katayose, Ryoji Takahashi, Kyuya Harada, Jun Abe

    Agronomy   3 ( 1 )   117 - 134   2013.2

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    DOI: 10.3390/agronomy3010117

  • Molecular characterization of two high-palmitic-acid mutant loci induced by X-ray irradiation in soybean Reviewed

    Toyoaki Anai, Tomoki Hoshino, Naoko Imai, Yutaka Takagi

    Breeding Science   61 ( 5 )   631 - 638   2012.12

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    DOI: 10.1270/jsbbs.61.631

  • ダイズ変異体ライブラリーの作出と利用

    穴井 豊昭

    育種学研究 (別冊)   14 ( 2 )   17   2012.9

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  • ダイズ突然変異体リソースの開発と利用

    穴井 豊昭

    日本遺伝学会第84回大会 プログラム・予稿集   83   2012.9

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  • Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering Reviewed

    Zhengjun Xia, Satoshi Watanabe, Tetsuya Yamada, Yasutaka Tsubokura, Hiroko Nakashima, Hong Zhai, Toyoaki Anai, Shusei Sato, Toshimasa Yamazaki, Shixiang Lu, Hongyan Wu, Satoshi Tabata, Kyuya Harada

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   109 ( 32 )   E2155 - E2164   2012.8

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    DOI: 10.1073/pnas.1117982109

  • NBRPミヤコグサ・ダイズにおけるダイズリソースの概要について

    穴井豊昭

    九州大学 有体物管理センター シンポジウム   2012.2

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  • Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean Reviewed

    ANAI T.

    Breed. Sci.   61 ( 1 )   462 - 467   2012.1

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    Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean
    Mutant-based reverse genetics offers a powerful way to create novel mutant alleles at a selected locus. This approach makes it possible to directly identify plants that carry a specific modified gene from the nucleotide sequence data. Soybean [Glycine max (L.) Merr.] has a highly redundant paleopolyploid genome (approx. 1.1 Gb), which was completely sequenced in 2010. Using reverse genetics to support functional genomics studies designed to predict gene function would accelerate post-genomics research in soybean. Furthermore, the novel mutant alleles created by this approach would be useful genetic resources for improving various traits in soybean. A reverse genetic screening platform in soybean has been developed that combines more than 40,000 mutant lines with a high-throughput method, Targeting Local Lesions IN Genome (TILLING). In this review, the mutant-based reverse genetic approach based on this platform is described, and the likely evolution of this approach in the near future.

    DOI: 10.1270/jsbbs.61.462

  • The National BioResource Project (NBRP) Lotus and Glycine in Japan Reviewed

    Hashiguchi Masatsugu, Abe Jun, Aoki Toshio, ANAI Toyoaki, SUZUKI Akihiro, AKASHI Ryo

    Breeding science   61 ( 1 )   453 - 461   2012.1

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    The National BioResource Project (NBRP) Lotus and Glycine in Japan

  • TILLING法によるダイズ青色光受容体CRY1突然変異体の単離

    庄山亜矢子, 穴井豊昭

    第6回九州育種談話会   2011.11

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  • 大豆品種「トヨシロメ」に見出された高ショ糖遺伝子の特性

    穴井豊昭, 井上大輔, 古賀晴香

    第6回九州育種談話会   2011.11

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  • TILLING法によるダイズGmPhyA1突然変異体の単離とその開花に及ぼす影響

    上原誉史, 穴井豊昭

    第6回九州育種談話会   2011.11

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  • Molecular characterization and marker development of mid-oleic-acid mutant M23 for the development of high-oleic cultivars of soybean Reviewed

    Tomoki Hoshino, Nobuhisa Kawashita, Yutaka Takagi, Toyoaki Anai

    PLANT BREEDING   130 ( 5 )   544 - 550   2011.10

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    DOI: 10.1111/j.1439-0523.2011.01871.x

  • 烏骨鶏と白色レグホーンにおけるプロラクチン遺伝子領域周辺の連鎖不平衡ブロックについて Reviewed

    岳 佳妮, 松田 莉朋, ローシャン ジャーハン, 下桐 猛, 穴井 豊昭, 和田 康彦

    日本家禽学会誌   48 ( J2 )   J63 - J68   2011.10

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    白色レグホーンのプロラクチン遺伝子のプロモーター領域には,烏骨鶏や赤色野鶏には認められていない24-bpの挿入が存在する。また,大分県畜産試験場の烏骨鶏選抜集団第4世代の311羽にはIn/In型の個体は存在しないことが報告されている。そこで,本研究では烏骨鶏の就巣性と産卵率の改良を目的として,プロラクチン遺伝子領域の周辺に1塩基多型マーカーを開発し,烏骨鶏と他の鶏品種との間での連鎖不平衡ブロックについて検討した。24-bpの挿入の有無に加えて,プロラクチン遺伝子の転写開始点上流(cPR01d;-3764)と転写終了点下流(cPR05b;+607,cPR02b;+3643)の3か所の1塩基多型について,日本各地から収集した烏骨鶏53羽,日本在来品種19羽,レイヤー23羽,その他の品種22羽の遺伝子型判定を行った。その結果,cPR02bでは烏骨鶏の遺伝子型はGG37羽,GA14羽,AA2羽であり,レイヤーではGG0羽GA1羽,AA22羽であった。cPR05bにおいても烏骨鶏でGG36羽,GC13羽,CC4羽であり,レイヤーでは全個体がAA型であった。烏骨鶏とレイヤーとの間のx2値はcPR01dで24.0,ln/Del変異で76.0,cPR05bで59.9,cPR02bで62.9とすべて有意であることからプロラクチン遺伝子領域周辺に連鎖不平衡ブロックが存在することが明らかとなった。一方,烏骨鶏とその他の品種の間のx2値は全てのマーカーで有意ではなかった。しかし烏骨鶏と日本在来品種との間ではIn/Del変異とcPR05bにおいて5&#37;水準で有意であった。また,レイヤーとその他の品種の間およびレイヤーと日本在来品種の間のx2値は全て1&#37;水準で有意であった。

  • 植物生理化学研究と遺伝子解析法

    星野友紀, 穴井豊昭

    「最新 植物生理化学」 長谷川宏司・広瀬克利 編. 大学教育出版   306 - 322   2011.10

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  • Molecular characterization and marker development of mid-oleic-acid mutant M23 for the development of high-oleic cultivars of soybean Reviewed

    Tomoki Hoshino, Nobuhisa Kawashita, Yutaka Takagi, Toyoaki Anai

    PLANT BREEDING   130 ( 5 )   544 - 550   2011.10

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    DOI: 10.1111/j.1439-0523.2011.01871.x

  • TILLING法に利用できるダイズ突然変異体リソース Reviewed

    穴井豊昭

    第29回日本植物細胞分子生物学会(福岡)大会・シンポジウム講演要旨集   54 - 54   2011.9

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  • ダイズ品種「トヨシロメ」に見出された高ショ糖遺伝子の特性

    穴井豊昭, 井上大輔, 古賀晴香

    育種学研究   13 ( 別2 )   215 - 215   2011.9

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  • A Map-Based Cloning Strategy Employing a Residual Heterozygous Line Reveals that the GIGANTEA Gene Is Involved in Soybean Maturity and Flowering Reviewed

    Satoshi Watanabe, Zhengjun Xia, Rumiko Hideshima, Yasutaka Tsubokura, Shusei Sato, Naoki Yamanaka, Ryoji Takahashi, Toyoaki Anai, Satoshi Tabata, Keisuke Kitamura, Kyuya Harada

    GENETICS   188 ( 2 )   395 - U260   2011.6

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    DOI: 10.1534/genetics.110.125062

  • Molecular characterization of two high-palmitic-acid mutant loci induced by X-ray irradiation in soybean Reviewed

    Toyoaki Anai, Tomoki Hoshino, Naoko Imai, Yutaka Takagi

    BREEDING SCIENCE   61 ( 5 )   631 - 638   2011.1

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    DOI: 10.1270/jsbbs.61.631

  • The National BioResource Project (NBRP) Lotus and Glycine in Japan Reviewed

    Masatsugu Hashiguchi, Jun Abe, Toshio Aoki, Toyoaki Anai, Akihiro Suzuki, Ryo Akashi

    BREEDING SCIENCE   61 ( 5 )   453 - 461   2011.1

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    DOI: 10.1270/jsbbs.61.453

  • Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean Reviewed

    Toyoaki Anai

    BREEDING SCIENCE   61 ( 5 )   462 - 467   2011.1

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    DOI: 10.1270/jsbbs.61.462

  • Novel GmFAD2-1b mutant alleles created by reverse genetics induce marked elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles Reviewed

    Tomoki Hoshino, Yutaka Takagi, Toyoaki Anai

    BREEDING SCIENCE   60 ( 4 )   419 - 425   2010.12

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    DOI: 10.1270/jsbbs.60.419

  • Phylogenetic relationships of Citrus and its relatives based on rbcL gene sequences Reviewed

    Tshering Penjor, Toyoaki Anai, Yukio Nagano, Ryoji Matsumoto, Masashi Yamamoto

    TREE GENETICS & GENOMES   6 ( 6 )   931 - 939   2010.12

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    DOI: 10.1007/s11295-010-0302-1

  • TILLING法を用いたダイズ品種「フクユタカ」からのFT3変異体の単離

    西美友紀, 木下剛仁, 穴井豊昭, 中島寿亀

    育種学研究   109 ( 別2 )   205 - 205   2010.9

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  • ダイズの矮性突然変異遺伝子についての解析

    穴井豊昭, 北原阿子

    育種学研究   109 ( 別2 )   77 - 77   2010.9

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  • X線照射によって誘発されたダイズ脂肪酸突然変異体におけるDNA変異形態の特徴

    星野友紀, 川下宣久, 高木胖, 穴井豊昭

    育種学研究   12 ( 別2 )   76 - 76   2010.9

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  • Agrobacterium-Mediated Transformation of Euphorbia tirucalli Callus Reviewed

    Hidenobu Uchida, Hirofumi Yamashita, Toyoaki Anai, Toshiya Muranaka, Kanji Ohyama

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   74 ( 4 )   851 - 853   2010.4

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    DOI: 10.1271/bbb.90783

  • ダイズ突然変異体ライブラリーの増幅を目的としたMultiple Displacement Amplification(MDA)法の最適化

    中島皓子, 穴井豊昭

    佐賀大学農学部彙報   95 ( 95 )   29 - 38   2010.1

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  • MDA法によって増幅を行ったダイズ突然変異体ライブラリーを鋳型としたTILLING法による変異の検出

    中島皓子, 穴井豊昭

    第4回九州育種談話会   2009.12

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  • 新規ダイズ突然変異体リソースとTILLINGによる変異体スクリーニング

    穴井豊昭

    NBRPシンポジウム「NBRPが提供するミュータントリソース」   2009.12

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  • ダイズ種子イソフラボン含量の改変を目指したUDP-グルコース:イソフラボン7-O-グルコシルトランスフェラーゼ突然変異体のスクリーニング

    浅田真央, 穴井豊昭

    第4回九州育種談話会   2009.12

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  • TILLING法を用いたダイズ(Glycine max)種子脂肪酸組成の代謝工学的改変

    穴井豊昭

    第30回種子生理生化学研究会年会 講演要旨集   2 - 3   2009.11

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  • MDA法によって増幅を行ったダイズ突然変異体ライブラリーを鋳型としたTILLING法による変異の検出

    中島皓子, 穴井豊昭

    育種学研究   11 ( 2 )   354   2009.9

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  • ダイズ開花期関連遺伝子座FT2のマップベースクローニング

    渡辺啓史, 夏正俊, 秀島瑠満子, 坪倉康隆, 佐藤修正, 山中直樹, 加賀秋人, 片寄裕一, 田畑哲之, 穴井豊昭, 原田久也

    育種学研究   11 ( 2 )   84   2009.9

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  • ダイズの根粒多着生変異体の選抜と評価

    北 淳二, 久野 裕子, 西原 芳弘, 穴井 豊昭, 有馬 進, 鈴木 章弘

    日本作物學會紀事   78   166 - 167   2009.9

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    DOI: 10.14829/jcsproc.228.0.166.0

  • TILLING法による超高オレイン酸ダイズ突然変異系統の開発

    穴井豊昭, 星野友紀

    育種学研究   11 ( 2 )   167   2009.9

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  • TILLING法によるダイズ突然変異体ライブラリーからのGmFAD3-2a変異体の単離

    星野友紀, 穴井豊昭

    育種学研究   11 ( 2 )   150   2009.9

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  • Map-Based Cloning of the Gene Associated With the Soybean Maturity Locus E3 Reviewed

    Satoshi Watanabe, Rumiko Hideshima, Zhengjun Xia, Yasutaka Tsubokura, Shusei Sato, Yumi Nakamoto, Naoki Yamanaka, Ryoji Takahashi, Masao Ishimoto, Toyoaki Anai, Satoshi Tabata, Kyuya Harada

    GENETICS   182 ( 4 )   1251 - 1262   2009.8

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    DOI: 10.1534/genetics.108.098772

  • ダイズ油脂中のオレイン酸含量を増加させる突然変異体及びその原因遺伝子

    穴井豊昭

    特許願 P09-0057   2009.6

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  • ダイズ遺伝資源中に見出されたFAD2遺伝子ファミリーの多様性

    穴井豊昭

    育種学研究(別)   11 ( 1 )   234   2009.3

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  • 突然変異体を活用したダイズ油脂組成の代謝工学的改良

    穴井豊昭

    第47回近畿アグリハイテクシンポジウム   2009.3

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  • Sequencing and Analysis of Approximately 40 000 Soybean cDNA Clones from a Full-Length-Enriched cDNA Library Reviewed

    Taishi Umezawa, Tetsuya Sakurai, Yasushi Totoki, Atsushi Toyoda, Motoaki Seki, Atsushi Ishiwata, Kenji Akiyama, Atsushi Kurotani, Takuhiro Yoshida, Keiichi Mochida, Mie Kasuga, Daisuke Todaka, Kyonoshin Maruyama, Kazuo Nakashima, Akiko Enju, Saho Mizukado, Selina Ahmed, Kyoko Yoshiwara, Kyuya Harada, Yasutaka Tsubokura, Masaki Hayashi, Shusei Sato, Toyoaki Anai, Masao Ishimoto, Hideyuki Funatsuki, Masayoshi Teraishi, Mitsuru Osaki, Takuro Shinano, Ryo Akashi, Yoshiyuki Sakaki, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

    DNA RESEARCH   15 ( 6 )   333 - 346   2008.12

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    DOI: 10.1093/dnares/dsn024

  • 突然変異体を利用したダイズ貯蔵脂質の改良

    穴井豊昭

    第3回九州育種談話会   2008.12

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  • TILLING法を用いたダイズミュータントライブラリーからのFT3突然変異系統の単離

    秀島瑠満子, 渡辺啓史, 原田久也, 穴井豊昭

    第3回九州育種談話会   2008.12

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  • MDA法によって増幅したDNAからの変異の検出

    中島皓子, 穴井豊昭

    第3回九州育種談話会   2008.12

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  • Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a

    Toyoaki Anai, Tomoko Yamada, Rumiko Hideshima, Takehito Kinoshita, Shaikh M. Rahman, Yutaka Takagi

    BREEDING SCIENCE   58 ( 4 )   447 - 452   2008.12

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    DOI: 10.1270/jsbbs.58.447

  • Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a Reviewed

    Toyoaki Anai, Tomoko Yamada, Rumiko Hideshima, Takehito Kinoshita, Shaikh M. Rahman, Yutaka Takagi

    BREEDING SCIENCE   58 ( 4 )   447 - 452   2008.12

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  • Sequencing and Analysis of Approximately 40 000 Soybean cDNA Clones from a Full-Length-Enriched cDNA Library

    Taishi Umezawa, Tetsuya Sakurai, Yasushi Totoki, Atsushi Toyoda, Motoaki Seki, Atsushi Ishiwata, Kenji Akiyama, Atsushi Kurotani, Takuhiro Yoshida, Keiichi Mochida, Mie Kasuga, Daisuke Todaka, Kyonoshin Maruyama, Kazuo Nakashima, Akiko Enju, Saho Mizukado, Selina Ahmed, Kyoko Yoshiwara, Kyuya Harada, Yasutaka Tsubokura, Masaki Hayashi, Shusei Sato, Toyoaki Anai, Masao Ishimoto, Hideyuki Funatsuki, Masayoshi Teraishi, Mitsuru Osaki, Takuro Shinano, Ryo Akashi, Yoshiyuki Sakaki, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

    DNA RESEARCH   15 ( 6 )   333 - 346   2008.12

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    DOI: 10.1093/dnares/dsn024

  • TILLING法によるダイズ突然変異体ライブラリーのスクリーニング

    穴井豊昭, 秀島瑠満子, 今井奈保子

    日本育種学会第114回講演会   2008.10

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  • ダイズ開花期関連遺伝子座FT3のマップベースクローニング

    渡辺啓史, 秀島瑠満子, 夏正俊, 坪倉康隆, 佐藤修正, 中山直樹, 石本政男, 田端哲之, 穴井豊昭, 原田久也

    日本育種学会第114回講演会   2008.10

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  • 大豆 オレリッチ50

    高木 胖, 穴井 豊昭

    2008.3

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  • An integrated high-density linkage map of soybean with RFLP, SSR, STS, and AFLP markers using a single F-2 population Reviewed

    Zhengjun Xia, Yasutaka Tsubokura, Masako Hoshi, Masayoshi Hanawa, Chizuru Yano, Kayo Okamura, Talaat A. Ahmed, Toyoaki Anai, Satoshi Watanabe, Masaki Hayashi, Takashi Kawai, Khwaja G. Hossain, Hirokazu Masaki, Kazumi Asai, Naoki Yamanaka, Nakao Kubo, Koh-ichi Kadowaki, Yoshiaki Nagamura, Masahiro Yano, Takuji Sasaki, Kyuya Harada

    DNA RESEARCH   14 ( 6 )   257 - 269   2007.12

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    DOI: 10.1093/dnares/dsm027

  • Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation Reviewed

    Yukio Nagano, Syoko Takao, Takahiro Kudo, Ei'ichi Iizasa, Toyoaki Anai

    PLANT CELL REPORTS   26 ( 12 )   2111 - 2117   2007.12

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  • X-線照射によるダイズ突然変異体ライブラリーの開発

    秀島瑠満子, 佐藤奈々絵, 松元美紀, 池田勝志, 穴井豊昭

    日本育種学会第112回講演会   2007.9

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  • 組換え型CEL1エンドヌクレアーゼの発現

    今井奈保子, 秀島瑠満子, 穴井豊昭

    日本育種学会第112回講演会   2007.9

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  • Hemiphragma heterophyllumの組織培養と二次代謝物生産 Reviewed

    森 暁美, 藤岡 稔大, 吉田 都, 穴井 豊昭, 石丸 幹二

    日本食品化学学会誌   13 ( 1 )   29 - 34   2006.5

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    Shoot culture of Hemiphragma heterophyllum (Scrophulariaceae) was established for the first time. Six known compounds, plantamajoside, hemiphroside B, 2&#039;, 6"-O-diacetylplantamajoside, globularin, iso-scrophurarioside and cinnamic acid were isolated from the shoot tissues of H. heterophyllum. 2&#039;, 6"-O-diacetylplantamajoside, which was isolated from this genius for the first time, is important chemotaxonomic marker suggesting the relationship between Hemiphragma and Wulfenia. Hairy root culture of H. heterophyllum was also established. In the hairy root cultures, high concentration of plantamajoside and hemiphroside B was observed. A phenolic compound p-tyrosol, which was not detected in the parent plantlets, was also isolated from the hairy roots. H. heterophyllum has a potential to become a new useful material for functional secondary metabolites in the field of food ingredients.

    DOI: 10.18891/jjfcs.13.1_29

  • Identification of corresponding genes for three low-alpha-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed Reviewed

    T Anai, T Yamada, T Kinoshita, SM Rahman, Y Takagi

    PLANT SCIENCE   168 ( 6 )   1615 - 1623   2005.6

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    DOI: 10.1016/j.plantsci.2005.02.016

  • Analysis of Molecular Diversity in Water Chestnut Based on RAPD Markers Reviewed

    A. Hoque ., T. Anai ., S. Arima .

    Biotechnology(Faisalabad)   4 ( 2 )   144 - 148   2005.3

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    DOI: 10.3923/biotech.2005.144.148

  • Three novel soybean germplasms with unique fatty acid composition using multiple mutant alleles Reviewed

    SM Rahman, T Anai, T Kinoshita, S Arima, Y Takagi

    BREEDING SCIENCE   54 ( 3 )   225 - 229   2004.9

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  • RNA silencing of the introduced coat protein gene of Turnip mosaic virus confers broad-spectrum resistance in transgenic Arabidopsis Reviewed

    Ken Nomura, Kazusato Ohshima, Toyoaki Anai, Hidetoshi Uekusa, Nobuhiro Kita

    Phytopathology   94 ( 7 )   730 - 736   2004.7

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    DOI: 10.1094/PHYTO.2004.94.7.730

  • 大豆種子で発現する遺伝子の同定と連鎖分析 (〔不二たん白質研究振興財団〕第6回研究報告会記録)

    原田 久也, 穴井 豊昭

    大豆たん白質研究   6 ( 24 )   18 - 21   2003.10

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  • Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene Reviewed

    T Anai, M Koga, H Tanaka, T Kinoshita, SM Rahman, Y Takagi

    PLANT CELL REPORTS   21 ( 10 )   988 - 992   2003.6

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    DOI: 10.1007/s00299-003-0609-6

  • A novel soybean germplasm with elevated saturated fatty acids Reviewed

    SM Rahman, T Anai, T Kinoshita, Y Takagi

    CROP SCIENCE   43 ( 2 )   527 - 531   2003.3

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  • イネ芽生えにおいてジベレリン処理により発現が制御されている遺伝子群の探索

    西 美友紀, 高木 胖, 穴井 豊昭, 高木 胖, 穴井 豊昭

    佐賀大学農学部彙報   87 ( 87 )   95 - 103   2002.12

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    植物の背丈は、イネをはじめとする穀物の生産性に関わる重要な形質の一つである。植物ホルモンの一種であるジベレリン類(GAs)は、細胞の分裂と伸長を促進することにより、植物の背丈に関与する最も効果的な成長調節因子として働いている。ジベレリン類の中間体とその代謝経路については詳細な研究が行われているが、細胞に存在する受容体や細胞内情報伝達経路の詳細については未だ完全に明らかにされているとは言えない。本研究において、我々はジベレリン情報伝達経路に関する遺伝子の同定を目的として、GA3処理を行ったイネ芽生えを材料としたサブトラクションを行い、ジベレリンによって発現が制御される候補遺伝子の探索を行った。最初に、PCR-サブトラクションにより14クローンが得られた。これらのクローンの発現パターンをノーザンブロット分析により調査したところ、最終的に、4つのクローンで発現の上昇が認められたが、各々のクローンでは異なる発現パ

  • 7.1 生理活性物質(分子・構造・作用・受容体・分布・容量など)

    長谷川, 宏治, 鈴木, 隆久, 穴井, 豊昭

    石原勝敏 [ほか] 編集委員代表「生物学データ大百科事典(下)」,朝倉書店   1591-1601   2002.9

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  • Combining ability in loci for high oleic and low linolenic acids in soybean Reviewed

    SM Rahman, T Kinoshita, T Anai, Y Takagi

    CROP SCIENCE   41 ( 1 )   26 - 29   2001.1

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    DOI: 10.2135/cropsci2001.41126x

  • A pair of invertedly repeated genes in Chlamydomonas reinhardtii encodes a zygote-specific protein whose expression is UV-sensitive Reviewed

    H Uchida, L Suzuki, T Anai, K Doi, H Takano, H Yamashita, T Oka, S Kawano, KI Tomizawa, T Kawazu, H Kuroiwa, T Kuroiwa

    CURRENT GENETICS   36 ( 4 )   232 - 240   1999.10

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  • Genetic relationships between loci for palmitate contents in soybean mutants Reviewed

    SM Rahman, T Kinoshita, T Anai, Y Takagi

    JOURNAL OF HEREDITY   90 ( 3 )   423 - 428   1999.5

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  • Development of a reduced linolenate soy mutant by Re-irradiation and its genetic analysis Reviewed

    Y Takagi, SM Rahman, T Anai, SK Wasala, T Kinoshita, M Khalekuzzaman

    BREEDING SCIENCE   49 ( 1 )   1 - 5   1999.3

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  • Establishment of framework P1 clones for map-based cloning and genome sequencing: direct RFLP mapping of large clones Reviewed

    D Shibata, M Seki, N Mitsukawa, N Hayashida, T Ito, T Taji, T Tsuge, M Matsui, T Anai, YG Liu, RF Whittier, K Shinozaki

    GENE   225 ( 1-2 )   31 - 38   1998.12

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    DOI: 10.1016/S0378-1119(98)00534-4

  • Inter-locus relationship between genes controlling palmitic acid contents in soybean mutants Reviewed

    T Kinoshita, SM Rahman, T Anai, Y Takagi

    BREEDING SCIENCE   48 ( 4 )   377 - 381   1998.12

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  • The biological roles of small GTPases and interacting proteins in plants Reviewed

    H Uchimiya, T Anai, ET Aspuria, M Matsui, A Nakano, T Ueda

    JOURNAL OF PLANT RESEARCH   111 ( 1102 )   257 - 260   1998.6

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  • Genetic relationships of soybean mutants for different linolenic acid contents Reviewed

    SM Rahman, T Kinoshita, T Anai, S Arima, Y Takagi

    CROP SCIENCE   38 ( 3 )   702 - 706   1998.5

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    DOI: 10.2135/cropsci1998.0011183X003800030014x

  • AtGDI2, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor Reviewed

    T Ueda, T Yoshizumi, T Anai, M Matsui, H Uchimiya, A Nakano

    GENE   206 ( 1 )   137 - 143   1998.1

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  • Isolation and characterization of an auxin-binding protein gene from radish, and its expression in insect cells Reviewed

    T Anai, R Takai, M Miyata, H Uchida, S Kosemura, S Yamamura, R Ishizaki, K Hasegawa

    PHYSIOLOGIA PLANTARUM   101 ( 3 )   606 - 611   1997.11

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    DOI: 10.1034/j.1399-3054.1997.1010322.x

  • Comparison of abp1 primary sequences from monocotyledonous and dicotyledonous species Reviewed

    T Anai, M Miyata, S Kosemura, S Yamamura, T Tsuge, M Matsui, H Uchida, K Hasegawa

    JOURNAL OF PLANT PHYSIOLOGY   151 ( 4 )   446 - 449   1997.10

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  • Design of photoaffinity reagents for labeling the auxin receptor in maize Reviewed

    Seiji Kosemura, Hideyuki Emori, Shosuke Yamamura, Toyoaki Anai, Kaori Tomita, Koji Hasegawa

    Tetrahedron Letters   38 ( 12 )   2125 - 2128   1997.3

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    DOI: 10.1016/S0040-4039(97)00323-7

  • Ara4 GTPase AND ITS INTERACTANTS Reviewed

    UEDA Takashi, MATSUDA Noriyuki, ANAI Toyoaki, TSUKAYA Hirokazu, UCHIMIYA Hirofumi, NAKANO Akihiko

    Plant and cell physiology   38   s122   1997.3

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    Ara4 GTPase AND ITS INTERACTANTS

  • An arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase Reviewed

    T Ueda, N Matsuda, T Anai, H Tsukaya, H Uchimiya, A Nakano

    PLANT CELL   8 ( 11 )   2079 - 2091   1996.11

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  • An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase Reviewed

    Takashi Ueda, Noriyuki Matsuda, Toyoaki Anai, Hirokazu Tsukaya, Hirofumi Uchimiya, Akihiko Nakano

    Plant Cell   8 ( 11 )   2079 - 2091   1996.11

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    DOI: 10.1105/tpc.8.11.2079

  • Brefeldin A induces the accumulation of unusual membrane structures in elongating pollen tubes of Nicotiana tabacum L. Reviewed

    T Ueda, H Tsukaya, T Anai, A Hirata, K Hasegawa, H Uchimiya

    JOURNAL OF PLANT PHYSIOLOGY   149 ( 6 )   683 - 689   1996.11

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  • A new auxin-inhibiting substance, 4-Cl-6,7-dimethoxy-2-benzoxazolinone, from light-grown maize shoots Reviewed

    T Anai, H Aizawa, N Ohtake, S Kosemura, S Yamamura, K Hasegawa

    PHYTOCHEMISTRY   42 ( 2 )   273 - 275   1996.5

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  • Characterization and subcellular localization of a small GTP binding protein (Ara-4) from Arabidopsis: Conditional expression under control of the promoter of the gene for heat-shock protein HSP81-1 Reviewed

    T Ueda, T Anai, H Tsukaya, A Hirata, H Uchimiya

    MOLECULAR & GENERAL GENETICS   250 ( 5 )   533 - 539   1996.3

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    DOI: 10.1007/s004380050106

  • Structure activity relationship of lepidimoide and its analogues Reviewed

    K Yamada, T Anai, S Kosemura, S Yamamura, K Hasegawa

    PHYTOCHEMISTRY   41 ( 3 )   671 - 673   1996.2

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  • ISOLATION AND CHARACTERIZATION OF 4-CHLORO-6,7-DIMETHOXYBENZOXAZOLIN-2-ONE A NEW AUXIN-INHIBITING BENZOXAZOLINONE FROM ZEA-MAYS Reviewed

    S KOSEMURA, H EMORI, S YAMAMURA, T ANAI, H AIZAWA, N OHTAKE, K HASEGAWA

    CHEMISTRY LETTERS   1995 ( 11 )   1053 - 1054   1995.11

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  • Sodium chloride stimulates adenylate kinase level in seedlings of salt-sensitive rice varieties Reviewed

    PK Samarajeewa, M Kawai, T Anai, A Hirai, H Uchimiya

    JOURNAL OF PLANT PHYSIOLOGY   147 ( 2 )   277 - 280   1995.11

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  • IMMUNOLOGICAL ANALYSIS OF A SMALL GTP-BINDING PROTEIN IN HIGHER-PLANT CELLS Reviewed

    T ANAI, ET ASPURIA, N FUJII, T UEDA, M MATSUI, K HASEGAWA, H UCHIMIYA

    JOURNAL OF PLANT PHYSIOLOGY   147 ( 1 )   48 - 52   1995.10

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  • LEPIDIMOIDE, AN ALLELOPATHIC SUBSTANCE IN THE EXUDATES FROM GERMINATED SEEDS Reviewed

    K YAMADA, T ANAI, K HASEGAWA

    PHYTOCHEMISTRY   39 ( 5 )   1031 - 1032   1995.7

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  • PHENOTYPIC INSTABILITY OF TRANSGENIC TOBACCO PLANTS AND THEIR PROGENIES EXPRESSING ARABIDOPSIS-THALIANA SMALL GTP-BINDING PROTEIN GENES Reviewed

    ET ASPURIA, T ANAI, N FUJII, T UEDA, M MIYOSHI, M MATSUI, H UCHIMIYA

    MOLECULAR & GENERAL GENETICS   246 ( 4 )   509 - 513   1995.2

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  • 植物のGタンパク質と細胞内情報伝達

    穴井豊昭, 内宮博文

    植物の遺伝子発現(内宮博文、長田俊之 編)   108 - 114   1995.2

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  • CHEMICAL STUDIES ON 2,4-DIHYDROXY-7-METHOXY-2H-1,4-BENZORAZIN-3(4H)-ONE IN CONNECTION WITH 6-METHOXY-2-BENZOXAZOLINONE, AN AUXIN-INHIBITING SUBSTANCE OF ZEA-MAYS L Reviewed

    S KOSEMURA, S YAMAMURA, T ANAI, K HASEGAWA

    TETRAHEDRON LETTERS   35 ( 44 )   8221 - 8224   1994.10

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  • IN-VITRO MUTATION ANALYSIS OF ARABIDOPSIS-THALIANA SMALL GTP-BINDING PROTEINS AND DETECTION OF GAP-LIKE ACTIVITIES IN PLANT-CELLS Reviewed

    T ANAI, M MATSUI, N NOMURA, R ISHIZAKI, H UCHIMIYA

    FEBS LETTERS   346 ( 2-3 )   175 - 180   1994.6

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  • MOLECULAR-STRUCTURE OF RAS-RELATED SMALL GTP-BINDING PROTEIN GENES OF RICE PLANTS AND GTPASE ACTIVITIES OF GENE-PRODUCTS IN ESCHERICHIA-COLI Reviewed

    S KIDOU, T ANAI, M UMEDA, S AOTSUKA, T TSUGE, A KATO, H UCHIMIYA

    FEBS LETTERS   332 ( 3 )   282 - 286   1993.10

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  • Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana Reviewed International journal

    Toyoaki Anai, Koji Hasegawa, Yuichiro Watanabe, Hirofumi Uchimiya, Ryotaro Ishizaki, Minami Matsui

    GENE   108 ( 2 )   259 - 264   1991.12

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    Isolation and characterization of the soybean Sg-3 gene that is involved in genetic variation in sugar chain composition at the C-3 position in soyasaponins

    DOI: 10.1016/0378-1119(91)90442-E

  • IDENTIFICATION OF 2 CELL-CYCLE-CONTROLLING CDC2 GENE HOMOLOGS IN ARABIDOPSIS-THALIANA Reviewed

    T HIRAYAMA, Y IMAJUKU, T ANAI, M MATSUI, A OKA

    GENE   105 ( 2 )   159 - 165   1991.9

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  • IDENTIFICATION OF 2 CELL-CYCLE-CONTROLLING CDC2 GENE HOMOLOGS IN ARABIDOPSIS-THALIANA Reviewed

    T HIRAYAMA, Y IMAJUKU, T ANAI, M MATSUI, A OKA

    GENE   105 ( 2 )   159 - 165   1991.9

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  • Identification and characterization of a major QTL underlying soybean isoflavone malonylglycitin content Invited Reviewed International journal

    Satoshi Watanabe, Risa Yamada, Hazuki Kanetake, Akito Kaga, Toyoaki Anai

    BREEDING SCIENCE   69 ( 4 )   564 - 572   1900

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    DOI: 10.1270/jsbbs.19027

  • Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean Invited Reviewed International journal

    Kaori Hirata, Kyoko Takagi, Tetsuya Yamada, Takashi Sayama, Toyoaki Anai, Akio Kikuchi, Masao Ishimoto

    BREEDING SCIENCE   69 ( 2 )   345 - 351   1900

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    DOI: 10.1270/jsbbs.18091

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Books

  • 代替タンパク質の技術と市場 第1章-1 植物由来代替肉の現状と今後

    穴井豊昭( Role: Joint author)

    CMC出版  2024.5 

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    Language:Japanese   Book type:Scholarly book

  • 豆類の百科事典 第II章-39 豆類の突然変異育種

    穴井豊昭( Role: Joint author)

    朝倉書店  2024.5 

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    Language:Japanese   Book type:Scholarly book

  • 植物バイオテクノロジーでめざすSDGs ―変わる私たちの食と薬 3章 タンパク質危機を救う植物由来の代替肉

    穴井豊昭( Role: Joint author)

    化学同人  2023.6 

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    Language:Japanese   Book type:General book, introductory book for general audience

  • 植物バイオテクノロジーでめざすSDGs : 変わる私たちの食と薬

    穴井 豊昭(小泉 望・加藤 晃 編)( Role: Contributor第3章 タンパク質危機を救う植物由来の代替肉)

    化学同人  2022.12    ISBN:9784759820867

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    Total pages:ix, 168p, 図版 [4] p   Language:Japanese   Book type:General book, introductory book for general audience

    CiNii Books

    researchmap

  • 植物育種学 第5版 北柴大泰・西尾剛 編文永堂出版 「第15章 食味および品質と成分」

    穴井 豊昭( Role: Joint author)

    2021.9 

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    Responsible for pages:p293-309   Language:Japanese   Book type:Scholarly book

  • 世界人口を支える持続可能な農業

    穴井 豊昭

    大学教育出版  2019.7 

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    Language:Japanese  

  • 有用作物の作成

    穴井豊昭

    2017.2 

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  • Mutant-based reverse genetics for functional genomics of non-model crops

    2016.3 

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    Mutant-based reverse genetics for functional genomics of non-model crops

  • Mutation Breeding for Fatty Acid Composition in Soybean

    Yutaka Takagi, Toyoaki Anai, Hitoshi Nakagawa( Role: Joint author)

    2012.9 

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  • Development of novel fatty acid composition in soybean oil through induced mutation

    Y. Takagi, T. Anai, H. Nakagawa( Role: Joint author)

    CABI Publishers  2012.1 

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  • 植物生理化学研究と遺伝子解析法

    星野友紀, 穴井豊昭

    2011.10 

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  • Positional cloning of the responsible genes for maturity loci E1, E2 and E3 in soybean

    K. Harada, S. Watanabe, Z. Xia, Y. Tsubokura, N. Yamanaka, T. Anai( Role: Joint author)

    InTech Publishers  2011.8 

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  • 7.1 生理活性物質(分子・構造・作用・受容体・分布・容量など)

    長谷川, 宏治, 鈴木, 隆久, 穴井, 豊昭

    2002.9 

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  • 植物のGタンパク質と細胞内情報伝達

    穴井豊昭, 内宮博文

    講談社サイエンティフィク  1995.2 

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Presentations

  • Isolation and utilization of novel soybean mutants from high-density mutant population. Invited International conference

    Toyoaki Anai

    OPTICS & PHOTONICS International Congress 2022  2023.4 

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    Event date: 2023.4 - 2023.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Development and characterization of early heading mutant lines of glutinous rice variety ‘Hiyokumochi’.

    MINN YA, 鄭紹輝, 穴井豊昭, 藤田大輔

    日本育種学会 第143回講演会  2023.3 

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    Event date: 2023.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:静岡大学(静岡市)   Country:Japan  

  • ダイズ油脂に含まれるフラン酸合成に関与する遺 伝子の同定と利用

    大曲絢子, 山田梨裟, 江頭燦実華, 海口直弥, 高濱宏郁, 元丸潤郁, 松本茜, 駿河奈那美, 木村祐太, 牧田成人, 岡本裕樹, 岡部遼, 佐野貴士, 佐藤俊郎, 鈴木基孝, 齋藤三四郎, 穴井豊昭, 渡邊啓史

    日本育種学会 第143回講演会  2023.3 

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡大学(静岡市)   Country:Japan  

  • ベンタゾン薬剤に対するダイズの高度感受性遺伝子の単離

    加藤信、横田侑子、鈴木倫太郎、藤澤由紀子、佐山貴司、加賀秋人、穴井豊昭、小松邦彦、大木信彦、菊池彰夫、石本政男

    日本育種学会 第143回講演会  2023.3 

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡大学(静岡市)   Country:Japan  

  • 植物性タンパク質の利用促進に向けたダイズ品質の改良 Invited

    穴井 豊昭

    日本生物工学会 創立100周年記念シンポジウム 生物工学の未来 -未来の“食”と“食料生産”への展望-  2023.2 

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    Event date: 2023.2 - 2022.2

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

    Repository Public URL: https://hdl.handle.net/2324/7172664

  • ダイズで植物肉はどこまで美味しくできる? Invited

    穴井 豊昭

    JBA “未来へのバイオ技術”勉強会SDGsと未来食シリーズ4「次世代肉をさらに美味しく!自由に!」  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • 代替肉の現状と原料となるダイズに望まれている特性. Invited

    穴井 豊昭

    日本植物バイオテクノロジー学会 市民公開シンポジウム  2022.9 

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    Event date: 2022.8 - 2022.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:大阪公立大学(堺市)   Country:Japan  

  • 1細胞ゲノム情報を用いた根圏微生物叢の変動解析

    木伏真子, 西川洋平, 細川正人, 井手圭吾, 小川雅人, 穴井豊昭, 竹山春子

    日本土壌微生物学会大会  2022.6 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 窒素固定増強遺伝子を導入したフクユタカの優位性

    鈴木章弘, 西田雄輝, 菅沼教生, 渡邊啓史, 穴井豊昭, 有馬進, 富永晃好

    第253回 日本作物学会講演会  2022.3 

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    Event date: 2022.3

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • ダイズ種子中のイソフラボン合成に関与するQTL、qMGly-11の責任遺伝子の同定

    山田梨裟, 前田朱里, 堀谷正樹、 穴井豊昭, 渡邊啓史

    日本育種学会第141回講演会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ダイズイソフラボン合成関連遺伝子の時期特異的発現について

    濱崎英史, 栗原志夫, 栗山朋子, 穴井 豊昭, 竹山 春子, 松井 南

    日本農芸化学会2022年度大会  2022.3 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 代替肉素材としてのダイズの 有用性と望まれる特性 Invited

    穴井豊昭

    令和3年度 日本栄養・食糧学会 九州・沖縄支部大会  2021.10 

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ダイズの成長に伴う根圏微生物叢の変動解析

    木伏真子, 西川洋平, 細川正人, 井手圭吾, 小川雅人, 穴井豊昭, 竹山春子

    第73回日本生物工学会大会  2021.10 

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 突然変異体を用いたダイズ油脂成分の改変 Invited

    穴井豊昭

    日本食品工学会 第68回大会講演会  2021.8 

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    Event date: 2021.8

    Language:Japanese  

    Country:Japan  

  • A3-16 イヌビエ芽生えから放出される植物選択阻害活性物質

    瀬尾 茂美, 穴井 豊昭, 長谷川 宏司

    植物化学調節学会研究発表記録集  1994.10 

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  • A3-15 発芽種子から分泌されるアレロパシー活性における有機物、無機物の貢献度

    津布楽 洋和, 田中 宥司, 塩家 仙子, 山田 小須弥, 穴井 豊昭, 長谷川 宏司

    植物化学調節学会研究発表記録集  1994.10 

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  • A3-14 トウモロコシの光屈性に関する制御物質の化学的研究

    小瀬村 誠治, 山村 庄亮, 穴井 豊明, 長谷川 宏司

    植物化学調節学会研究発表記録集  1994.10 

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  • CONTRIBUTION OF ORGANIC AND INORGANICSUBSTANCES TO THE ALLELOPATHIC ACTIVITY IN THE EXUDATES OFGERMINATED SEEDS

    TSUBURA Hirokazu, TANAKA Hiroshi, ISHIGURO Yukio, SHIOYA Hisao, ANAI Toyoaki, HASEGAWA Koji

    Plant and cell physiology  1995.3 

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    CONTRIBUTION OF ORGANIC AND INORGANICSUBSTANCES TO THE ALLELOPATHIC ACTIVITY IN THE EXUDATES OFGERMINATED SEEDS

  • 緑化ヒマワリ芽生えからの新規成長抑制物質単離の試み

    相澤 裕子, 加藤 潤, 九島 みどり, 浅川 知香子, 穴井 豊昭, 長谷川 宏司

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan  1995.9 

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  • 発芽種子から放出されるアレロパシー物質

    九島 みどり, 穴井 豊昭, 長谷川 宏司

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan  1995.9 

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  • 光誘導性成長抑制物質による遺伝子発現調節機構

    穴井 豊昭, 相澤 裕子, 河野 直子, 宮田 百, 小瀬村 誠治, 山村 庄亮, 長谷川 宏司

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan  1995.9 

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  • B-23 種子発芽におけるレピジモイドの消長

    山田 小須弥, 穴井 豊昭, 長谷川 宏司

    植物化学調節学会研究発表記録集  1995.10 

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  • PHUSIOLOGICAL FUNCTION OF LEPIDIMOIDE

    YAMADA Kosumi, ANAI Toyoaki, YOKOTANI-TOMITA Kaori, HASEGAWA Koji

    Plant and cell physiology  1996.3 

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    PHUSIOLOGICAL FUNCTION OF LEPIDIMOIDE

  • LIGHT-INDUCED PLANT GROWTH INHIBITORS FROM SUNFLOWER(HELIANTHUS ANNUUSL. )SEEDLINGS

    KATO Jun, AIZAWA Hiroko, KOSEMURA Seiji, YAMAMURA Shosuke, ANAI Toyoaki, YOKOTANI-TOMITA Kaori, HASEGAWA Koji

    Plant and cell physiology  1996.3 

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    LIGHT-INDUCED PLANT GROWTH INHIBITORS FROM SUNFLOWER(HELIANTHUS ANNUUSL. )SEEDLINGS

  • EFFECT OF PHOTOTROPISM-REGULATED GROWTH INHIBITORS FOR AUXIN-BINDING ACTIVITY OF MEMBRANE ASSOCIATED AUXINBINDING PROTEIN(ABP1)

    AIZAWA Hiroko, ANAI Toyoaki, KOSEMURA Seiji, YAMAMURA Shosuke, HASEGAWA Koji

    Plant and cell physiology  1996.3 

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    EFFECT OF PHOTOTROPISM-REGULATED GROWTH INHIBITORS FOR AUXIN-BINDING ACTIVITY OF MEMBRANE ASSOCIATED AUXINBINDING PROTEIN(ABP1)

  • CLONING AND CHARACTERIZATION OF RICE SUCROSE-PHOSPHATE SYNTHASE

    TOKURAKU Kiyotaka, ANAI Toyoaki, HASEGAWA Koji, HISAJIMA Sigeru

    Plant and cell physiology  1996.3 

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    CLONING AND CHARACTERIZATION OF RICE SUCROSE-PHOSPHATE SYNTHASE

  • アベナおよびダイコンからのabp1遺伝子の単離と解析

    穴井 豊昭, 河野 直子, 宮田 百, 小瀬村 誠治, 山村 庄亮, 長谷川 宏司

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan  1996.10 

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  • 8 ダイコンSAUR遺伝子の単離と解析

    河野 直子, 穴井 豊昭, 宮田 百, 小瀬村 誠治, 山村 庄亮, 長谷川 宏司

    植物化学調節学会研究発表記録集  1996.10 

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  • 10 オーキシンレセプターの単離のための光アフィニティーラベル試薬の設計

    江守 秀之, 小瀬村 誠治, 山村 庄亮, 穴井 豊昭, 長谷川 宏司

    植物化学調節学会研究発表記録集  1996.10 

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  • 放射線再照射による低リノレン酸ダイズ突然変異体の開発とその遺伝解析

    高木 胖, Rahman S.M., 穴井 豊昭, Wasala S.K., 木下 剛仁, Khalekuzzaman M.

    育種学研究  1999.3 

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    Country:Japan  

  • これからの遺伝子組み換え植物

    穴井 豊昭

    佐賀大学農学部農場報告  2001.1 

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  • これからの遺伝子組み換え植物

    穴井 豊昭

    佐賀大学農学部農場報告  2001.1 

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  • P-37 アイスプラント(Mesembryanthemum crystallinum L.)形質転換体作出法の確立

    東江 栄, 梅本 真紀子, 砂川 春樹, 牧志 佑子, 穴井 豊昭, 野瀬 昭博

    日本作物學會紀事  2004.10 

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  • P-38 マクロアレイ法による遺伝子発現解析からみたイネ紋枯病抵抗性系統の特性

    東江 栄, 中村 淳哉, 野瀬 昭博, 穴井 豊昭, 和佐野 喜久夫

    日本作物學會紀事  2005.3 

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  • 大豆 オレリッチ50

    高木 胖, 穴井 豊昭

    2008.3 

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  • ダイズ開花期関連遺伝子座FT3のマップベースクローニング

    渡辺啓史, 秀島瑠満子, 夏正俊, 坪倉康隆, 佐藤修正, 中山直樹, 石本政男, 田端哲之, 穴井豊昭, 原田久也

    日本育種学会第114回講演会  2008.10 

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  • TILLING法によるダイズ突然変異体ライブラリーのスクリーニング

    穴井豊昭, 秀島瑠満子, 今井奈保子

    日本育種学会第114回講演会  2008.10 

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  • 突然変異体を利用したダイズ貯蔵脂質の改良

    穴井豊昭

    第3回九州育種談話会  2008.12 

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  • TILLING法を用いたダイズミュータントライブラリーからのFT3突然変異系統の単離

    秀島瑠満子, 渡辺啓史, 原田久也, 穴井豊昭

    第3回九州育種談話会  2008.12 

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  • MDA法によって増幅したDNAからの変異の検出

    中島皓子, 穴井豊昭

    第3回九州育種談話会  2008.12 

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  • 高窒素固定能を示すダイズ変異体の選抜および評価

    鈴木 章弘, 久野 裕子, 北 淳二, 穴井 豊昭, 有馬 進

    日本作物學會紀事  2009.3 

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  • 突然変異体を活用したダイズ油脂組成の代謝工学的改良

    穴井豊昭

    第47回近畿アグリハイテクシンポジウム  2009.3 

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  • ダイズ遺伝資源中に見出されたFAD2遺伝子ファミリーの多様性

    穴井豊昭

    育種学研究(別)  2009.3 

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  • ダイズ油脂中のオレイン酸含量を増加させる突然変異体及びその原因遺伝子

    穴井豊昭

    特許願 P09-0057  2009.6 

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  • ダイズ開花期関連遺伝子座FT2のマップベースクローニング

    渡辺啓史, 夏正俊, 秀島瑠満子, 坪倉康隆, 佐藤修正, 山中直樹, 加賀秋人, 片寄裕一, 田畑哲之, 穴井豊昭, 原田久也

    育種学研究  2009.9 

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  • TILLING法による超高オレイン酸ダイズ突然変異系統の開発

    穴井豊昭, 星野友紀

    育種学研究  2009.9 

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  • TILLING法によるダイズ突然変異体ライブラリーからのGmFAD3-2a変異体の単離

    星野友紀, 穴井豊昭

    育種学研究  2009.9 

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  • MDA法によって増幅を行ったダイズ突然変異体ライブラリーを鋳型としたTILLING法による変異の検出

    中島皓子, 穴井豊昭

    育種学研究  2009.9 

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  • TILLING法を用いたダイズ(Glycine max)種子脂肪酸組成の代謝工学的改変

    穴井豊昭

    第30回種子生理生化学研究会年会 講演要旨集  2009.11 

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  • 新規ダイズ突然変異体リソースとTILLINGによる変異体スクリーニング

    穴井豊昭

    NBRPシンポジウム「NBRPが提供するミュータントリソース」  2009.12 

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  • ダイズ種子イソフラボン含量の改変を目指したUDP-グルコース:イソフラボン7-O-グルコシルトランスフェラーゼ突然変異体のスクリーニング

    浅田真央, 穴井豊昭

    第4回九州育種談話会  2009.12 

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  • The Diversity of Isoflavone contents and In Vitro Antioxidant Activities in Japanese Soybean (Glycine Max (L.) Merr.) Cultivars.

    2010.1 

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    The Diversity of Isoflavone contents and In Vitro Antioxidant Activities in Japanese Soybean (Glycine Max (L.) Merr.) Cultivars.

  • ダイズの矮性突然変異遺伝子についての解析

    穴井豊昭, 北原阿子

    育種学研究  2010.9 

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  • X線照射によって誘発されたダイズ脂肪酸突然変異体におけるDNA変異形態の特徴

    星野友紀, 川下宣久, 高木胖, 穴井豊昭

    育種学研究  2010.9 

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  • TILLING法を用いたダイズ品種「フクユタカ」からのFT3変異体の単離

    西美友紀, 木下剛仁, 穴井豊昭, 中島寿亀

    育種学研究  2010.9 

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  • 根粒の共生窒素固定能及び収量が増加したダイズ変異体の選抜

    西原 芳弘, 久野 裕子, 穴井 豊昭, 有馬 進, 鈴木 章弘

    日本作物學會紀事  2011.3 

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  • ダイズ品種「トヨシロメ」に見出された高ショ糖遺伝子の特性

    穴井豊昭, 井上大輔, 古賀晴香

    育種学研究  2011.9 

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  • TILLING法に利用できるダイズ突然変異体リソース

    穴井豊昭

    第29回日本植物細胞分子生物学会(福岡)大会・シンポジウム講演要旨集  2011.9 

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  • 大豆品種「トヨシロメ」に見出された高ショ糖遺伝子の特性

    穴井豊昭, 井上大輔, 古賀晴香

    第6回九州育種談話会  2011.11 

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  • TILLING法によるダイズGmPhyA1突然変異体の単離とその開花に及ぼす影響

    上原誉史, 穴井豊昭

    第6回九州育種談話会  2011.11 

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  • TILLING法によるダイズ青色光受容体CRY1突然変異体の単離

    庄山亜矢子, 穴井豊昭

    第6回九州育種談話会  2011.11 

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  • NBRPミヤコグサ・ダイズにおけるダイズリソースの概要について

    穴井豊昭

    九州大学 有体物管理センター シンポジウム  2012.2 

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  • ダイズ突然変異体リソースの開発と利用

    穴井 豊昭

    日本遺伝学会第84回大会 プログラム・予稿集  2012.9 

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  • ダイズ変異体ライブラリーの作出と利用

    穴井 豊昭

    育種学研究 (別冊)  2012.9 

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  • ダイズ根粒PBS溶液が根粒菌に誘導する共生窒素固定反応のcDNAマイクロアレイ法による評価(2013年度大会一般講演要旨)

    横山 正, 小林 究, 永田 真紀, 大津 直子, 鈴木 章弘, 穴井 豊昭

    土と微生物  2014.4 

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  • NBRPミヤコグサ・ダイズ―日本に自生するツルマメ遺伝資源の種子成分について―

    橋口正嗣, WEE Chi‐Do, 大庭千佳, 石垣元気, 田中秀典, 原田久也, 阿部純, 青木俊夫, 穴井豊昭, 鈴木章弘, 明石良, 明石良

    日本作物学会講演会要旨集  2017.3 

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  • 2step‐TILLINGを用いた自家不和合植物ハツカダイコンの突然変異体選抜法

    上妻馨梨, 上妻馨梨, 千葉元子, 千葉元子, 穴井豊昭, 上田実希, 小口理一, 花田耕介, 花田耕介, 彦坂幸毅, 彦坂幸毅, 藤井伸治, 藤井伸治

    育種学研究  2017.3 

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    Country:Other  

  • 人口知能及び深層学習を組み合わせた画像解析による大豆白変葉の検出可能性

    星野祐輝, 長沼俊介, 穴井豊昭, 田中宗浩

    九州農業試験研究機関協議会研究発表会発表要旨集(Web)  2017.8 

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    Country:Other  

  • ダイズRXP遺伝子へのT挿入がダイズ葉焼病圃場抵抗性をもたらす

    田口文緒, 高橋浩司, 鈴木倫太郎, 横田侑子, 清水武彦, 山崎俊正, 山田哲也, 小木曽映里, 佐山貴司, 佐山貴司, 山田直弘, 山田直弘, 大木信彦, 穴井豊昭, 片寄裕一, 加賀秋人, 石本政男

    育種学研究  2017.10 

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    Country:Other  

  • マメ科植物における窒素固定関連遺伝子SEN1の多型と表現型

    鈴木章弘, 鈴木章弘, 江上由佳, 河野里実, 中尾隆寛, 千々岩諒汰, 中島菜摘, 原田克哉, 河済英紀, 渡邊啓史, 渡邊啓史, 穴井豊昭, 穴井豊昭, 菅沼教生, 有馬進, 有馬進

    日本作物学会講演会要旨集  2018.3 

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    Country:Other  

  • 高密度誘発変異系統群とバルク分析法を用いたダイズ草型制御遺伝子群の探索

    平賀勧, 山川博幹, 山川博幹, 山田哲也, 平田香里, 島村聡, 菊池彰夫, 穴井豊昭, 加賀秋人, 石本政男

    育種学研究  2018.9 

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    Country:Other  

  • 植物育種に向けた1塩基多型遺伝子座のHRM分析における頑健性を最大化するための選択基準

    山形悦透, 吉村淳, 穴井豊昭, 渡邊啓史

    育種学研究  2018.9 

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    Country:Other  

  • ダイズ高密度誘発突然変異体ライブラリーを用いたソヤサポニン組成変異体の探索と評価

    KRISHNAMURTHY Panneerselvam, 藤澤由紀子, 向山恭介, 高橋裕也, 阿部華子, 山根健太郎, 伊藤綾香, 平賀勧, 加賀秋人, 穴井豊昭, 塚本知玄, 石本政男

    育種学研究  2018.9 

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  • マメ科植物の窒素固定関連遺伝子SEN1の多型と表現型

    鈴木章弘, 江上由佳, 河野里実, 中尾隆寛, 千々岩諒汰, 中島菜摘, 原田克哉, 河済英紀, 渡邊啓史, 穴井豊昭, 有馬進, 菅沼教生

    植物微生物研究会研究交流会講演要旨集  2018.11 

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  • Creation of soybean cultivars with enhanced nitrogen fixation

    Shimomura Aya, Nishida Yuki, Takemura Kirin, Suganuma Norio, Watanabe Satoshi, Anai Toyoaki, Arima Susumu, Tominaga Akiyoshi, Onjo Michio, Suzuki Akihiro

    Abstracts of Meeting of the CSSJ  2023.3  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.255.0_167

    CiNii Research

  • Stage-specific isoflavones synthesis genes expression profile in lateral root of soybean growing in differential type of soils

    Hamasaki Hidefumi, Kurihara Yukio, Kuriyama Tomoko, Makita Yuko, Kawauchi Masaharu, Takashi Kenjyo, Kojima Katsuhiro, Anai Toyoaki, Takeyama Haruko, Matsui Minami

    Abstracts of Meeting of the CSSJ  2023.9  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.256.0_112

    CiNii Research

  • ラマン分光法による微生物コロニーからの二次代謝産物のスクリーニング手法の開発

    諏訪 駿之介, 安藤 正浩, 中島 琢自, 堀井 俊平, 松本 厚子, 穴井 豊昭, 竹山 春子

    日本生物工学会大会講演要旨集  2023.8  (公社)日本生物工学会

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  • Evaluation for Phenotypic Diversity of High-density Mutant Populations in Soybean

    Miura Hina, Anai Toyoaki

    Abstracts of Meeting of the CSSJ  2023.9  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.256.0_110

    CiNii Research

  • Effect of enhanced nitrogen fixation gene on growth and yield in soybean

    Shimomura Aya, Nishida Yuki, Yamamoto Shion, Suganuma Norio, Watanabe Satoshi, Anai Toyoaki, Arima Susumu, Tominaga Akiyoshi, Suzuki Akihiro

    Abstracts of Meeting of the CSSJ  2024.3  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.257.0_142

    CiNii Research

  • Screening for oligosaccharide mutant lines from soybean mutant populations.

    Kawakubo Naomi, Kono Masaki, Fukuyama Tomoaki, Watanabe Satoshi, Anai Toyoaki

    Abstracts of Meeting of the CSSJ  2023.9  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.256.0_109

    CiNii Research

  • Analysis of Isoflavone Accumulation Patterns in the Lateral Root of Soybean Mutant Lines

    Kondo Takeshi, Anai Toyoaki, Watanabe Satoshi

    Abstracts of Meeting of the CSSJ  2023.9  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.256.0_111

    CiNii Research

  • Cultivation of enhanced nitrogen fixation soybean using amino acid polymorphisms in SEN1

    Shimomura Aya, Nishida Yuki, Yamamoto Shion, Suganuma Norio, Watanabe Satoshi, Anai Toyoaki, Arima Susumu, Tominaga Akiyoshi, Suzuki Akihiro

    Abstracts of Meeting of the CSSJ  2024.9  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.258.0_90

    CiNii Research

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MISC

  • 【香りの再現について】ダイズの脂肪酸から発生する主要な「不快臭」とそれらの遺伝的な改良 Invited

    穴井 豊昭

    におい・かおり環境学会誌   55 ( 3 )   171 - 175   2024.5   ISSN:1348-2904

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    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)   Publisher:(公社)におい・かおり環境協会  

    ダイズは世界的にも重要なマメ科商業作物のひとつであり,乾燥種子中に重量で約40%のタンパク質と約20%の脂質を含んでいる.しかし,この油を構成している特定の脂肪酸の酸化により発生する一連の「不快臭」が,ダイズ油を含む様々なダイズ食品において問題となっている.本稿では,ダイズの脂肪酸に由来する「不快臭」の発生メカニズムについて解説するとともに,ダイズ脂肪酸組成の遺伝学的改良とその「不快臭」低減への応用についての我々の最近の研究成果を紹介する.(著者抄録)

  • ダイズ油中の脂肪酸の遺伝的改良による不快臭の軽減と植物肉素材としての可能性

    穴井 豊昭

    オレオサイエンス   2023.6

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  • ダイズ油中の脂肪酸の遺伝的改良による不快臭の軽減と植物肉素材としての可能性

    穴井豊昭,

    オレオサイエンス   2023.6

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  • Improvement of soybean seed oil quality using fatty acid mutants Invited

    Anai Toyoaki

    Nippon Shokuhin Kagaku Kogaku Kaishi   70 ( 1 )   47 - 51   2023.1   ISSN:1341027X eISSN:18816681

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    <p>Soybean [<i>Glycine max</i> L. (Merr.)] is an important legume crop, with an annual global production of 367.8 million tons. The seeds contain about 20% oil of dry weight, which is mainly used for oil extraction, and the improvement of soybean oil quality is a critical subject world-wide. We isolated several fatty acid accumulation mutants and identified the corresponding genes. Next, we attempted to improve the soybean oil quality using these mutant genes. We successfully developed a novel high-oleic acid soybean cultivar, "SADAIEICHIOICHIGOU", from which oil with an oleic acid content as high as 80 % of total fatty acids was obtained. Moreover, the linoleic acid content of "SADAIEICHIOICHIGOU" was obviously low. The oil extracted from this cultivar showed higher oxidation stability than conventional varieties and had decreased levels of the unpleasant green off-odor of soybeans. In addition, we isolated a novel mutant that does not accumulate furan fatty acids, associated with a hay-like odor, and identified the corresponding gene. This series of soybean fatty acid mutants have great potential to improve the nutritional value, functionality, palatability, and oxidative stability of soybean-based food.</p>

    DOI: 10.3136/nskkk.nskkk-d-22-00056

    Web of Science

    Scopus

    CiNii Research

  • 突然変異体を用いたダイズ油脂成分改変

    穴井 豊昭

    日本食品工学会誌   2023.1

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    DOI: 10.3136/nskkk.NSKKK-D-22-0005

  • Analysis of soil microbial spatiotemporal variation in laboratory and field soybean

    木伏真子, 木伏真子, 西川洋平, 西川洋平, 細川正人, 細川正人, 細川正人, 細川正人, 井手圭吾, 井手圭吾, 小川雅人, 穴井豊昭, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本農芸化学会大会講演要旨集(Web)   2022   2022   ISSN:2186-7976

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    J-GLOBAL

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  • ダイズ突然変異体リソースの整備と新規アリルの開発に関する研究

    穴井 豊昭

    育種学研究   2020.12

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    DOI: 10.1270/jsbbr.20J18

  • 大豆油脂の機能と遺伝的改良

    穴井 豊昭

    アグリバイオ   2020.11

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  • ダイズ突然変異体リソースの開発とその活用

    穴井 豊昭

    2016.11

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  • ダイズ突然変異体ライブラリーの増幅を目的としたMultiple Displacement Amplification(MDA)法の最適化

    中島皓子, 穴井豊昭

    佐賀大学農学部彙報   2010.1

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  • 遺伝子の組み合わせによる高飽和脂肪酸組成となるダイズ系統の育成

    高木 胖, ラーマン S. M., 穴井 豊昭, 木下 剛仁

    育種学研究 = Breeding research   2002.8

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  • ダイズの高オレイン酸と低リノレン酸となる遺伝子の組合わせ

    高木 胖, ラーマン S.M., 木下 剛仁, 穴井 豊昭

    育種学研究 = Breeding research   2000.4

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  • ダイズ小胞体型ω-3脂肪酸不飽和化酵素遺伝子を導入したイネの作出とその脂肪酸組成の解析

    穴井 豊昭, 古賀 農人, 田中 寛法, ラーマン S. M., 高木 胖

    育種学研究 = Breeding research   2000.4

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  • 各種植物における膜結合型オーキシン結合タンパク質(ABP1)の構造解析

    宮田 百, 穴井 豊昭, 横谷 香織, 長谷川 宏司

    雑草研究   1996.4

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  • 緑化ヒマワリの光生長抑制物質

    加藤 潤, 相澤 裕子, 九島 みどり, 穴井 豊昭, 富田-横谷 香織, 小瀬村 誠治, 山村 庄亮, 長谷川 宏司

    雑草研究   1996.4

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  • 発芽種子および幼植物から分泌されるアレロパシー物質

    佐々 奈緒美, 穴井 豊昭, 富田-横谷 香織, 津布楽 洋和, 田中 宥司, 長谷川 宏司

    雑草研究   1996.4

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  • イネショ糖燐酸合成酵素のcDNAクローニング

    徳楽 清孝, 穴井 豊昭, 長谷川 宏司, 久島 繁

    日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan   1995.9

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  • 光照射したトウモロコシ芽生えから単離された抗オーキシン活性物質

    穴井 豊昭, 相澤 裕子, 小瀬村 誠治, 山村 庄亮, 長谷川 宏司

    雑草研究   1995.4

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  • ESTABLISHMENT OF MODEL SYSTEM FORANALYZING INTRACELLULAR TRANSPORT OF THE PLANT CELLS

    UEDA Takashi, ANAI Toyoaki, TSUKAYA Hirokaza, HASEGAWA Koji, UCHIMIYA Hirofumi

    Plant and cell physiology   1995.3

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    Language:English  

    ESTABLISHMENT OF MODEL SYSTEM FORANALYZING INTRACELLULAR TRANSPORT OF THE PLANT CELLS

  • ISOLATION AND CHARACTERIZATION OFANTI-AUXIN SUBSTANCES IN LIGHT-TREATED MAIZE SEEDLINGS

    ANAI Toyoaki, OHTAKE Nobuyuki, KOSEMURA Seiji, YAMAMURA Shosuke, HASEGAWA Koji

    Plant and cell physiology   1995.3

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    ISOLATION AND CHARACTERIZATION OFANTI-AUXIN SUBSTANCES IN LIGHT-TREATED MAIZE SEEDLINGS

  • ISOLATION AND ANALYSIS OF CDNAS ENCODING SMALL GTP-BINDING PROTEINS OF ARABIDOPSIS-THALIANA

    T ANAI, K HASEGAWA, Y WATANABE, H UCHIMIYA, R ISHIZAKI, M MATSUI

    GENE   1991.12

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Works

  • 品種登録 第29603号. 大豆 佐大HO1号

    穴井豊昭、渡邊啓史

    2023.4

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  • 大豆 佐大HO1号

    穴井 豊昭、渡邊 啓史

    2023.4

  • 大豆 SKT01

    穴井豊昭、渡邊啓史、他

    2023.3

  • 品種登録 第26085号. 大豆 佐賀黒7号

    2017.6

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  • ダイズ油脂中のオレイン酸含量を増加させる突然変異体及びその原因遺伝子

    穴井豊昭

    2009.6

  • 大豆 オレリッチ50

    高木 胖, 穴井 豊昭

    2008.3

  • 品種登録 第16459号. 大豆 オレリッチ50

    2008.3

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Industrial property rights

Patent   Number of applications: 1   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • 日本分子生物学会

  • 日本植物学会

  • 日本植物生理学会

  • 日本植物バイオテクノロジー学会

  • 日本育種学会

  • 植物生理化学会

  • 日本作物学会

  • 日本育種学会

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  • 日本植物生理学会

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  • 日本植物学会

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  • 日本植物バイオテクノロジー学会

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  • 日本分子生物学会

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  • 日本作物学会

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Committee Memberships

  • Councilor   Domestic

    2021.7 - Present   

  • 日本育種学会   日本育種学会和文誌 育種学研究 編集委員   Domestic

    2008.10 - Present   

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  • 日本育種学会   日本育種学会英文誌 Breeding Science 編集委員   Domestic

    2008.10 - Present   

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Academic Activities

  • Screening of academic papers

    Role(s): Peer review

    2024

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

    Number of peer-reviewed articles in Japanese journals:1

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:2

  • 未来社会創造事業 評価委員

    Role(s): Review, evaluation

    科学技術振興機構(JST)  2021.6 - 2021.9

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    Type:Scientific advice/Review 

  • Screening of academic papers

    Role(s): Peer review

    2021

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:5

    Number of peer-reviewed articles in Japanese journals:1

  • 育種学研究

    2009.1 - Present

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    Type:Academic society, research group, etc. 

  • Breeding Science

    2009.1 - Present

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Research Projects

  • 代謝工学による低イソフラボン含有ダイズの開発

    Grant number:24K08848  2024 - 2026

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    穴井 豊昭

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    Authorship:Principal investigator  Grant type:Scientific research funding

    本研究では、我々が開発したダイズ突然変異体を利用して、種子中および側根中でのイソフラボン組成および蓄積量の変化と根粒形成の変化の関係を明らかにし、根粒菌の着生に対するそれぞれのイソフラボン分子種の効果を解明するとともに感染に必要な閾値を明らかにすることで、低イソフラボンダイズの開発に必要な代謝経路改変技術を確立する。

    CiNii Research

  • 科学技術振興機構 戦略的創造研究推進事業 ALCA-Next 「変動環境適応型の共生窒素固定作物の開発」

    2023.11

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  • 突然変異体を用いた変動環境適応型ダイズ遺伝資源の開発と機能解析

    2023 - 2026

    ALCA-Next

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 農学研究院研究資金

    2023

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    Grant type:Donation

  • 高オレイン酸大豆の開発に関する共同研究

    2022.6 - 2023.3

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 各種大豆の品質評価に関する研究

    2022.6

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • ダイズオリゴ糖に関する研究

    2022.4

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 農学研究院研究資金

    2022

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    Grant type:Donation

  • 民間事業者等の種苗開発を支える「スマート育種システム」の開発

    2021.6 - 2022.3

    受託研究

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    Authorship:Coinvestigator(s)  Grant type:Other funds from industry-academia collaboration

  • 農学研究院研究資金

    2021

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    Grant type:Donation

  • 内閣府ムーンショット型農林水産研究開発事業 「土壌微生物叢アトラスに基づいた環境制御による循環型協生農業プラットフォーム構築」

    2020.6

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    Authorship:Coinvestigator(s) 

  • 土壌微生物叢アトラスに基づいた環境制御による循環型協生農業プラットフォーム構築

    2020 - 2024

    内閣府ムーンショット型農林水産研究開発事業

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 窒素固定増強遺伝子によるマメ科作物の低炭素投入型への転換

    Grant number:20H02890  2020 - 2023

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ダイズにおけるフラン脂肪酸生合成経路の解明とその農業特性に与える影響に関する研究

    Grant number:20H02960  2020 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • イネ人為突然変異白葉枯病抵抗性遺伝子の同定と抵抗性機構の解明

    Grant number:19H02934  2019 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ダイズ突然変異集団を利用した変異遺伝子の高速マッピングシステムの構築

    Grant number:17K07604  2017 - 2020

    日本学術振興会  科学研究費助成事業  特別推進研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • シンクロトロン光と次世代シークエンサーを用いた効率的な欠失突然変異体単離系の開発

    Grant number:17K07605  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ダイズ窒素固定関連遺伝子SEN1の多様性と収量性に与える影響

    Grant number:25292014  2013 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 重複遺伝子を持つダイズフラボノイド代謝経路の転写因子を標的とした改変

    Grant number:25450009  2013 - 2015

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ミヤコグサ・ダイズリソースの国際的拠点整備-収集・保存・提供-

    2012 - 2016

    科学技術振興調整費 (文部科学省)

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    Authorship:Principal investigator  Grant type:Contract research

  • TILLING 法を用いたダイズ脂肪酸組成の代謝工学的改良

    Grant number:22580006  2010 - 2012

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 酵母相同組換え系を用いたダイズ特定遺伝子破壊系統の作出と逆遺伝学的応用

    Grant number:17580008  2005 - 2006

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 高等植物のカドミウム耐性・吸収能に関与する二価鉄吸収経路の解明

    Grant number:16380232  2004 - 2007

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • RNA干渉および多重発光マクロアレイを併用したダイズ脂質生合成遺伝子の網羅的解析

    Grant number:14760005  2002 - 2003

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 突然変異誘発および組み換えDNA技術を併用したダイズ油脂の改良に関する研究

    Grant number:13460005  2001 - 2002

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • アグロバクテリウムを用いたダイズの高効率形質転換系の開発

    Grant number:12760008  2000 - 2001

    科学研究費助成事業  奨励研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 分子マーカーを利用いた高オレイン酸ダイズ突然変異系統の選抜と育成

    Grant number:11660006  1999 - 2000

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 植物細胞において小胞輸送を調節している低分子量GTP結合蛋白質制御機構の研究

    Grant number:09740588  1997 - 1998

    科学研究費助成事業  奨励研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 光刺激で誘導される新規抗オーキシン活性物質MBOAにより制御される遺伝子群の解析

    Grant number:07780527  1995

    科学研究費助成事業  試験研究(A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Class subject

  • 作物資源生態学特論

    2024.12 - 2025.2   Winter quarter

  • 体験的農業生産学入門

    2024.10 - 2025.3   Second semester

  • 基幹教育セミナー

    2024.6 - 2024.8   Summer quarter

  • 農場実習Ⅰ

    2024.4 - 2025.3   Full year

  • 農場実習

    2024.4 - 2025.3   Full year

  • 農場実習Ⅴ

    2024.4 - 2025.3   Full year

  • 農場実習Ⅱ

    2024.4 - 2024.9   First semester

  • 農場実習Ⅲ

    2024.4 - 2024.9   First semester

  • 農場実習Ⅳ

    2024.4 - 2024.9   First semester

  • Fieldwork on Bioresource and Bioenvironment 1

    2024.4 - 2024.6   Spring quarter

  • 作物資源生態学特論

    2023.12 - 2024.2   Winter quarter

  • 作物資源生態学特論

    2023.12 - 2024.2   Winter quarter

  • 作物資源生態学特論

    2023.12 - 2024.2   Winter quarter

  • 作物資源生態学特論

    2023.12 - 2024.2   Winter quarter

  • 体験的農業生産学入門

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 体験的農業生産学入門

    2023.10 - 2024.3   Second semester

  • 農業科指導法Ⅱ

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 体験的農業生産学入門

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 体験的農業生産学入門

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 農業生物科学演習第一

    2023.10 - 2024.3   Second semester

  • 修士論文

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • 農業生物科学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 農場実習

    2023.4 - 2024.3   Full year

  • 農場実習Ⅴ

    2023.4 - 2024.3   Full year

  • 農場実習Ⅰ

    2023.4 - 2024.3   Full year

  • 修士論文

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • 農業生物科学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 農場実習

    2023.4 - 2024.3   Full year

  • 修士論文

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • 農業生物科学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 農場実習

    2023.4 - 2024.3   Full year

  • 修士論文

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • 農業生物科学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 農場実習

    2023.4 - 2024.3   Full year

  • 農場実習Ⅱ

    2023.4 - 2023.9   First semester

  • 農場実習Ⅲ

    2023.4 - 2023.9   First semester

  • 農場実習Ⅳ

    2023.4 - 2023.9   First semester

  • Fieldwork on Bioresource and Bioenvironment 1

    2023.4 - 2023.6   Spring quarter

  • Fieldwork on Bioresource and Bioenvironment 1

    2023.4 - 2023.6   Spring quarter

  • Fieldwork on Bioresource and Bioenvironment 1

    2023.4 - 2023.6   Spring quarter

  • Fieldwork on Bioresource and Bioenvironment 1

    2023.4 - 2023.6   Spring quarter

  • 作物資源生態学特論

    2022.12 - 2023.2   Winter quarter

  • 体験的農業生産学入門

    2022.10 - 2023.3   Second semester

  • 農場実習Ⅴ

    2022.4 - 2023.3   Full year

  • 農場実習Ⅰ

    2022.4 - 2023.3   Full year

  • 農場実習

    2022.4 - 2023.3   Full year

  • 農場実習Ⅱ

    2022.4 - 2022.9   First semester

  • 農場実習Ⅲ

    2022.4 - 2022.9   First semester

  • 農場実習Ⅳ

    2022.4 - 2022.9   First semester

  • Fieldwork on Bioresource and Bioenvironment 1

    2022.4 - 2022.6   Spring quarter

  • 作物資源生態学特論

    2021.12 - 2022.2   Winter quarter

  • 体験的農業生産学入門

    2021.10 - 2022.3   Second semester

  • 農業生物科学演習第一

    2021.10 - 2022.3   Second semester

  • 農場実習Ⅰ

    2021.4 - 2022.3   Full year

  • 農場実習

    2021.4 - 2022.3   Full year

  • 農場実習Ⅱ

    2021.4 - 2021.9   First semester

  • 農場実習Ⅲ

    2021.4 - 2021.9   First semester

  • 農場実習Ⅳ

    2021.4 - 2021.9   First semester

  • 農場実習Ⅴ

    2021.4 - 2021.9   First semester

  • Fieldwork on Bioresource and Bioenvironment 1

    2021.4 - 2021.6   Spring quarter

▼display all

FD Participation

  • 2022.4   Role:Participation   Title:令和4年度 第1回全学FD(新任教員の研修)The 1st All-University FD (training for new faculty members) in FY2022

    Organizer:University-wide

  • 2021.11   Role:Participation   Title:農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2024  崇城大学大学院・工学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:集中

  • 2024  佐賀大学・農学部  Classification:Affiliate faculty  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:通年

  • 2023  佐賀大学・農学部  Classification:Affiliate faculty  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:通年

  • 2022  佐賀大学・農学部  Classification:Affiliate faculty  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:通年

  • 2022  崇城大学大学院・工学研究科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:集中

  • 2021  佐賀大学・農学部  Classification:Affiliate faculty  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:通年

▼display all

Social Activities

  • 佐賀県農業試験研究センター普通作物関係評価員

    Role(s): Report writing

    2008.4 - Present

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    専門的な立場から課題についてのアドバイスと評価を行った

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