Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.ajpath.2020.02.005

Repository Public URL： https://hdl.handle.net/2324/7173546

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.celrep.2018.09.010

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Brief summary of the current protocols for generating intestinal organoids
© 2018 Japanese Society of Developmental Biologists The intestine has fundamental functions for the maintenance of homeostasis, including food digestion and nutrient/water absorption. Although the lumen of the intestine is always exposed to pathogens, intestinal epithelial cells form monolayer sheets that act as an epithelial barrier to prevent the invasion of pathogens. Thus, disruption of the intestinal epithelial barrier causes inflammatory bowel diseases. To investigate the details of these intractable intestinal diseases, it is necessary to analyze the characteristics of intestinal epithelial cells in vitro. However, it is difficult to maintain and propagate intestinal epithelial cells in culture. Recently, intestinal organoid culture systems have been established, in which differentiated intestinal epithelial lineage cells can be continuously produced from intestinal stem cells and form epithelial organoids with crypt-like structures in long-term culture. Moreover, intestinal epithelial organoids can be generated not only from intestinal tissue-derived cells, embryonic stem cells, and induced pluripotent stem cells, but also by inducing direct conversion of nonintestinal somatic cells into intestinal epithelial cells. These intestinal organoids can be used in basic studies for understanding the mechanisms underlying intestinal development and diseases and will be applied in future transplantation therapy and drug discovery to treat intestinal diseases.

DOI： 10.1111/dgd.12559

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.stem.2017.08.020

Repository Public URL： https://hdl.handle.net/2324/7172621

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.stemcr.2016.11.002

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/srep34691

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/hep.28548

Language：English  

Rapid cell-fate conversion of mouse fibroblasts into hepatocyte-like cells
Recent progress in studies on direct cell-fate conversion of differentiated somatic cells into other cell types, which is known as "direct reprogramming", is expected to lead to innovations in health care. In our previous study, we found that three specific combinations of two transcription factors, comprising Hnf4α plus Foxa1, Foxa2, or Foxa3, were able to induce conversion of mouse fibroblasts into functional hepatocyte-like cells. These induced hepatocyte-like (iHep) cells will be useful for developing regenerative therapies for liver diseases and examining the pharmacological effects of drugs. However, to evaluate the potential utility of iHep cells, the phenomena involved in the direct conversion of fibroblasts into iHep cells should be examined in detail. Thus, in this study, we sequentially analyzed the early stage of fibroblast conversion into iHep cells after infection with retroviruses expressing Hnf4α and Foxa3. Our data demonstrated that the conversion into iHep cells began within 2 days after introduction of the transgenes into fibroblasts, and the number of iHep cells increased gradually as the culture progressed. The rapid cell-fate conversion of fibroblasts into iHep cells and stable expansion of iHep cells are two pieces of evidence suggesting the utility of iHep cells for cell transplantation therapy, bioartificial liver development, and screening of drugs for patients with liver diseases, which require many hepatocytes within a short period of time.

DOI： 10.2492/inflammregen.34.211

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Acquisition of lipid metabolic capability in hepatocyte-like cells directly induced from mouse fibroblasts.

DOI： 10.3389/fcell.2014.00043

Language：Japanese  

Country：Other  

Language：English  

Country：Other  

Analysis of hepatic lipid metabolism using iHep cells

Language：English  

Country：Other  

Analysis of reprogramming process from fibroblasts to induced hepatocyte-like cells

Language：Japanese  

Country：Other  

Language：English  

Country：Other  

nalysis of hepatic lipid metabolism using iHep cells

Language：English  

Country：Other  

Induction of functional hepatocytes from mouse fibroblasts

Language：Japanese  

Country：Other