Updated on 2024/07/28

Information

 

写真a

 
WATANABE SATOSHI
 
Organization
Medical Institute of Bioregulation Department of Molecular and Structural Biology Associate Professor
Graduate School of Systems Life Sciences Department of Systems Life Sciences(Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Tel
0926426833
Profile
分泌経路におけるタンパク質管理機構の構造生命科学 金属イオントランスポーターの構造機能解析 小胞体からの分泌機構の構造生命科学

Degree

  • PhD(Science)

Research History

  • 東北大学多元物質科学研究所 助教(2013年6月〜2024年3月)

Research Interests・Research Keywords

  • Research theme:Structural life study of protein quality control

    Keyword:protein quality control, chaperon, Metalloproteins, cargo receptor, cryo-EM

    Research period: 2024.4 - 2024.5

Awards

  • 進歩賞

    2011.11   日本結晶学会  

Papers

  • Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport Reviewed

    Satoshi Watanabe, Yoshiaki Kise, Kento Yonezawa, Mariko Inoue, Nobutaka Shimizu, Osamu Nureki, Kenji Inaba

    Nature Communications   15 ( 1 )   2024.3

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-024-46747-1

  • Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn2+ uptake into the Golgi apparatus Reviewed International journal

    Han, Ba Bui, Watanabe, S, Nomura, N, Liu, K, Uemura, T, Inoue, M, Tsutsumi, A, Fujita, H, Kinoshita, K. Kato, Y, Iwata, S, Kikkawa, M, Inaba, K

    Nature Communications   14 ( 1 )   4770 - 4770   2023.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn2+ uptake into the Golgi apparatus
    Zinc ions (Zn2+) are vital to most cells, with the intracellular concentrations of Zn2+ being tightly regulated by multiple zinc transporters located at the plasma and organelle membranes. We herein present the 2.2-3.1 Å-resolution cryo-EM structures of a Golgi-localized human Zn2+/H+ antiporter ZnT7 (hZnT7) in Zn2+-bound and unbound forms. Cryo-EM analyses show that hZnT7 exists as a dimer via tight interactions in both the cytosolic and transmembrane (TM) domains of two protomers, each of which contains a single Zn2+-binding site in its TM domain. hZnT7 undergoes a TM-helix rearrangement to create a negatively charged cytosolic cavity for Zn2+ entry in the inward-facing conformation and widens the luminal cavity for Zn2+ release in the outward-facing conformation. An exceptionally long cytosolic histidine-rich loop characteristic of hZnT7 binds two Zn2+ ions, seemingly facilitating Zn2+ recruitment to the TM metal transport pathway. These structures permit mechanisms of hZnT7-mediated Zn2+ uptake into the Golgi to be proposed.

    DOI: 10.1038/s41467-023-40521-5

  • Supersulphides provide airway protection in viral and chronic lung diseases Reviewed

    Nature Communications   14 ( 1 )   2023.7

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-023-40182-4

  • Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface. Reviewed International journal

    Yuta Amagai, Momo Yamada, Toshiyuki Kowada, Tomomi Watanabe, Yuyin Du, Rong Liu, Satoshi Naramoto, Satoshi Watanabe, Junko Kyozuka, Tiziana Anelli, Tiziana Tempio, Roberto Sitia, Shin Mizukami, Kenji Inaba

    Nature communications   14 ( 1 )   2683 - 2683   2023.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-023-38397-6

  • Cryo-EM structures of human SPCA1a reveal the mechanism of Ca 2+ /Mn 2+ transport into the Golgi apparatus Reviewed

    Zhenghao Chen, Satoshi Watanabe, Hironori Hashida, Michio Inoue, Yasukazu Daigaku, Masahide Kikkawa, Kenji Inaba

    Science Advances   9 ( 9 )   2023.3

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/sciadv.add9742

  • Multiple sub-state structures of SERCA2b reveal conformational overlap at transition steps during the catalytic cycle. Reviewed International journal

    Yuxia Zhang, Chigusa Kobayashi, Xiaohan Cai, Satoshi Watanabe, Akihisa Tsutsumi, Masahide Kikkawa, Yuji Sugita, Kenji Inaba

    Cell reports   41 ( 10 )   111760 - 111760   2022.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2022.111760

  • Cryo-EM analysis provides new mechanistic insight into ATP binding to Ca2+ -ATPase SERCA2b. Reviewed International journal

    Yuxia Zhang, Satoshi Watanabe, Akihisa Tsutsumi, Hiroshi Kadokura, Masahide Kikkawa, Kenji Inaba

    The EMBO journal   40 ( 19 )   e108482   2021.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.15252/embj.2021108482

  • Cryo-EM structures of SERCA2b reveal the mechanism of regulation by the luminal extension tail. Reviewed International journal

    Yuxia Zhang, Michio Inoue, Akihisa Tsutsumi, Satoshi Watanabe, Tomohiro Nishizawa, Kazuhiro Nagata, Masahide Kikkawa, Kenji Inaba

    Science advances   6 ( 33 )   eabb0147   2020.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/sciadv.abb0147

  • Structural Insight into [NiFe] Hydrogenase Maturation by Transient Complexes between Hyp Proteins. Reviewed International journal

    Kunio Miki, Haruyuki Atomi, Satoshi Watanabe

    Accounts of chemical research   53 ( 4 )   875 - 886   2020.4

     More details

    Language:English  

    DOI: 10.1021/acs.accounts.0c00022

  • High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering. Reviewed International journal

    Nakamura Y, Lin YC, Watanabe S, Liu YC, Katsuyama K, Kanehara K, Inaba K

    iScience   21   577 - 586   2019.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering.
    Arabidopsis FLOWERING LOCUS T (FT) is a pivotal component of florigen, a long-range mobile flowering signal. Here, we determined the 1.0 Å-resolution crystal structure of FT, a significantly higher-resolution crystal structure of FT than previously reported one (2.6 Å). The present crystallographic studies revealed 4 alternative configurations with the precise location of the surrounding water molecules. Using this structural data, computational docking simulation predicted the putative binding sites for phosphatidylcholine (PC), an endogenous ligand that interacts with FT to modulate flowering time. In vitro reconstitution of the lipid-protein interaction showed that mutations at two of the predicted sites significantly compromised the lipid binding ability of FT. In planta, one of the mutant FT proteins significantly affected FT function in flowering, emphasizing the involvement of PC binding in modulating FT function. Our structural, biochemical, and transgenic analyses reveal the molecular mechanism of PC binding in FT-mediated flowering time control.

    DOI: 10.1016/j.isci.2019.10.045

  • Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay. Reviewed

    Inoue M, Sakuta N, Watanabe S, Zhang Y, Yoshikaie K, Tanaka Y, Ushioda R, Kato Y, Takagi J, Tsukazaki T, Nagata K, Inaba K

    Cell reports   27 ( 4 )   1221 - 1230.e3   2019.4

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    Structural Basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Regulation via Transmembrane Helix Interplay.

    DOI: 10.1016/j.celrep.2019.03.106

  • Zinc regulates ERp44-dependent protein quality control in the early secretory pathway Reviewed

    Watanabe S, Amagai Y, Sannino S, Tempio T, Anelli T, Harayama M, Masui S, Sorrentino I, Yamada M, Sitia R, Inaba K, equally contributed, co-corresponding

    Nature Communications   10 ( 1 )   2019.2

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    Zinc regulates ERp44-dependent protein quality control in the early secretory pathway

    DOI: 10.1038/s41467-019-08429-1

  • Crystal structures of a [NiFe] hydrogenase large subunit HyhL in an immature state in complex with a Ni chaperone HypA. Reviewed

    Kwon S, Watanabe S, Nishitani Y, Kawashima T, Kanai T, Atomi H, Miki K, equally contributed

    Proceedings of the National Academy of Sciences of the United States of America   115 ( 27 )   7045 - 7050   2018.7

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    Crystal structures of a [NiFe] hydrogenase large subunit HyhL in an immature state in complex with a Ni chaperone HypA.
    Ni-Fe clusters are inserted into the large subunit of [NiFe] hydrogenases by maturation proteins such as the Ni chaperone HypA via an unknown mechanism. We determined crystal structures of an immature large subunit HyhL complexed with HypA from <italic>Thermococcus kodakarensis</italic>. Structure analysis revealed that the N-terminal region of HyhL extends outwards and interacts with the Ni-binding domain of HypA. Intriguingly, the C-terminal extension of immature HyhL, which is cleaved in the mature form, adopts a β-strand adjacent to its N-terminal β-strands. The position of the C-terminal extension corresponds to that of the N-terminal extension of a mature large subunit, preventing the access of endopeptidases to the cleavage site of HyhL. These findings suggest that Ni insertion into the active site induces spatial rearrangement of both the N- and C-terminal tails of HyhL, which function as a key checkpoint for the completion of the Ni-Fe cluster assembly.

    DOI: 10.1073/pnas.1801955115

  • Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Reviewed International journal

    Nature communications   8 ( 1 )   1177 - 1177   2017.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-017-01311-y

  • The Highly Dynamic Nature of ERdj5 Is Key to Efficient Elimination of Aberrant Protein Oligomers through ER-Associated Degradation Reviewed

    Ken-ichi Maegawa, Satoshi Watanabe, Kentaro Noi, Masaki Okumura, Yuta Amagai, Michio Inoue, Ryo Ushioda, Kazuhiro Nagata, Teru Ogura, Kenji Inaba

    STRUCTURE   25 ( 6 )   846 - +   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.str.2017.04.001

  • Preparation of Selenoinsulin as a Long‐Lasting Insulin Analogue Reviewed

    129 ( 20 )   5614 - 5618   2017.5

     More details

    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/ange.201701654

  • Preparation of Selenoinsulin as a Long-Lasting Insulin Analogue Reviewed

    Kenta Arai, Toshiki Takei, Masaki Okumura, Satoshi Watanabe, Yuta Amagai, Yuya Asahina, Luis Moroder, Hironobu Hojo, Kenji Inaba, Michio Iwaoka

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   56 ( 20 )   5522 - 5526   2017.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/anie.201701654

  • Structural basis of pH-dependent client binding by ERp44, a key regulator of protein secretion at the ER-Golgi interface Reviewed

    Satoshi Watanabe, Manami Harayama, Shingo Kanemura, Roberto Sitia, Kenji Inaba

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 16 )   E3224 - E3232   2017.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1621426114

  • Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5 Reviewed

    Ryo Ushioda, Akitoshi Miyamoto, Michio Inoue, Satoshi Watanabe, Masaki Okumura, Ken-ichi Maegawa, Kaiku Uegaki, Shohei Fujii, Yasuko Fukuda, Masataka Umitsu, Junichi Takagi, Kenji Inaba, Katsuhiko Mikoshiba, Kazuhiro Nagata

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 41 )   E6055 - E6063   2016.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1605818113

  • Crystal structure of a [NiFe] hydrogenase maturation protease HybD from Thermococcus kodakarensis KOD1 Reviewed

    Sunghark Kwon, Yuichi Nishitani, Satoshi Watanabe, Yoshinori Hirao, Tadayuki Imanaka, Tamotsu Kanai, Haruyuki Atomi, Kunio Miki

    PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS   84 ( 9 )   1321 - 1327   2016.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/prot.25070

  • Structural basis of a Ni acquisition cycle for [NiFe] hydrogenase by Ni-metallochaperone HypA and its enhancer Reviewed

    Satoshi Watanabe, Takumi Kawashima, Yuichi Nishitani, Tamotsu Kanai, Takehiko Wada, Kenji Inaba, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   112 ( 25 )   7701 - 7706   2015.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1503102112

  • Crystal structures of the carbamoylated and cyanated forms of HypE for [NiFe] hydrogenase maturation Reviewed

    Taiga Tominaga, Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    Proceedings of the National Academy of Sciences of the United States of America   110 ( 51 )   20485 - 20490   2013.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1313620110

  • Identification and Structure of a Novel Archaeal HypB for [NiFe] Hydrogenase Maturation Reviewed

    Daisuke Sasaki, Satoshi Watanabe, Rie Matsumi, Toshihisa Shoji, Ayako Yasukochi, Kenta Tagashira, Wakao. Fukuda, Tamotsu Kanai, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    JOURNAL OF MOLECULAR BIOLOGY   425 ( 10 )   1627 - 1640   2013.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jmb.2013.02.004

  • Crystal Structures of the HypCD Complex and the HypCDE Ternary Complex: Transient Intermediate Complexes during [NiFe] Hydrogenase Maturation Reviewed

    Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    STRUCTURE   20 ( 12 )   2124 - 2137   2012.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.str.2012.09.018

  • Structure of the [NiFe]-hydrogenase maturation protein HypF from Thermococcus kodakarensis KOD1 Reviewed

    Taiga Tominaga, Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS   68 ( Pt 10 )   1153 - 1157   2012.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309112036421

  • Structural basis of [NiFe] hydrogenase maturation by Hyp proteins Reviewed

    Satoshi Watanabe, Daisuke Sasaki, Taiga Tominaga, Kunio Miki

    BIOLOGICAL CHEMISTRY   393 ( 10 )   1089 - 1100   2012.10

     More details

    Language:English  

    DOI: 10.1515/hsz-2012-0197

  • Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 Reviewed

    Daisuke Sasaki, Satoshi Watanabe, Tamotsu Kanai, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   417 ( 1 )   192 - 196   2012.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2011.11.083

  • Crystal Structure of HypA, a Nickel-Binding Metallochaperone for [NiFe] Hydrogenase Maturation Reviewed

    Satoshi Watanabe, Takayuki Arai, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    JOURNAL OF MOLECULAR BIOLOGY   394 ( 3 )   448 - 459   2009.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jmb.2009.09.030

  • Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA Reviewed

    Satoshi Watanabe, Akiko Kita, Kazuo Kobayashi, Kunio Miki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 11 )   4121 - 4126   2008.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.0709188105

  • Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1 Reviewed

    Takayuki Arai, Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS   63 ( Pt 9 )   765 - 767   2007.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309107038833

  • Crystal structures of [NiFe] hydrogenase maturation proteins HypC, HypD, and HypE: Insights into cyanation reaction by thiol redox signaling Reviewed

    Satoshi Watanabe, Rie Matsumi, Takayuki Arai, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    MOLECULAR CELL   27 ( 1 )   29 - 40   2007.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.molcel.2007.05.039

  • Crystallization and preliminary X-ray crystallographic studies of the [NiFe] hydrogenase maturation proteins HypC and HypD Reviewed

    Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS   63 ( Pt 6 )   538 - 541   2007.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309107023391

  • Crystallization and preliminary X-ray crystallographic studies of the oxidative-stress sensor SoxR and its complex with DNA Reviewed

    Satoshi Watanabe, Akiko Kita, Kazuo Kobayashi, Yasuhiro Takahashi, Kunio Miki

    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS   62 ( Pt 12 )   1275 - 1277   2006.12

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1744309106048482

  • Crystal structure of atypical cytoplasmic ABC-ATPase SufC from Thermus thermophilus HB8 Reviewed

    S Watanabe, A Kita, K Miki

    JOURNAL OF MOLECULAR BIOLOGY   353 ( 5 )   1043 - 1054   2005.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jmb.2005.09.017

▼display all

Books

  • SERCA2bの構造解析

    渡部 聡, 稲葉謙次( Role: Joint author)

    クライオ電子顕微鏡ハンドブック  2023.1 

     More details

    Language:Others  

  • 細胞のタンパク質の品質を監査する仕組み

    渡部 聡, 稲葉謙次( Role: Joint author)

    放射光利用の手引き ―農水産・医療,エネルギー,環境,材料開発分野などへの応用―  2019.2 

     More details

    Language:Others  

  • Chapter 3.3 Structural Insights into Disulfide Bond Formation and Protein Quality Control in the Mammalian Endoplasmic Reticulum

    Okumura M, Watanabe S, Inaba K

    Oxidative Folding of Proteins: Basic Principles, Cellular Regulation and Engineering  2018.7 

     More details

    Language:Others  

    Chapter 3.3 Structural Insights into Disulfide Bond Formation and Protein Quality Control in the Mammalian Endoplasmic Reticulum

  • [2Fe-2S] Oxidative- Stress Sensor SoxR Bound to DNA

    Watanabe S, Miki K

    Handbook of Metalloproteins, Vol. 4  2011.5 

     More details

    Language:Others  

    [2Fe-2S] Oxidative- Stress Sensor SoxR Bound to DNA

Presentations

  • 亜鉛依存性シャペロンERp44による基質認識・解離の分子構造基盤

    渡部 聡, 木谷美思, 三宅杏美子, 天貝佑太, 稲葉謙次

    第46回日本分子生物学会年会  2023.12 

     More details

    Event date: 2023.12

    Language:Others  

    Country:Other  

  • 全長カーゴ受容体ERGIC-53と補助因子MCFD2との複合体のクライオ電顕構造

    渡部 聡, 木瀬孔明, 米澤健人, 清水伸隆, 濡木理, 稲葉謙次

    第44回日本分子生物学会年会  2021.12 

     More details

    Event date: 2021.12

    Language:Others  

    Country:Other  

  • 全長カーゴ受容体ERGIC-53と補助因子MCFD2との複合体の相関構造解析

    渡部聡, 木瀬孔明, 米澤健人, 清水伸隆, 濡木理, 稲葉謙次

    第59回日本生物物理学会年会  2021.11 

     More details

    Event date: 2021.11

    Language:Others  

    Country:Other  

  • 亜鉛に依存したERp44-クライアント複合体の解離機構の解明

    渡部 聡、三宅杏美子、天貝佑太、稲葉謙次

    第21回日本蛋白質科学会年会  2021.6 

     More details

    Event date: 2021.6

    Language:Others  

    Country:Other  

  • ヒト由来亜鉛トランスポーターZnT7による亜鉛輸送機構の構造基盤

    渡部 聡

    第28回 INGEM & ToMMoセミナーシリーズ  2023.10 

     More details

    Language:Others  

    Country:Other  

  • クライオ電子顕微鏡で明らかになったカーゴ受容体ERGIC-53の柔らかな全長構造 Invited

    渡部 聡, 木瀬孔明, 米澤健人, 清水伸隆, 濡木理, 稲葉謙次

    第22回蛋白質科学会年会  2022.6 

     More details

    Language:Others  

    Country:Other  

  • Structural basis of zinc-dependent protein quality control in the early secretory pathway Invited International conference

    Satoshi Watanabe, Yuta Amagai, Sara Sannino, Momo Yamada, Roberto Sitia, Kenji Inaba

    Gordon Research Conference, Cell Biology of Metals  2019.7 

     More details

    Language:English  

    Country:Other  

    Structural basis of zinc-dependent protein quality control in the early secretory pathway

  • 亜鉛イオンとシャペロンタンパク質ERp44による新たなタンパク質品質管理機構 Invited

    渡部 聡, 天貝佑太, 山田桃, Roberto Sitia, 稲葉謙次

    第19回日本蛋白質科学会年会  2019.6 

     More details

    Language:Japanese  

    Country:Other  

  • タンパク質はどのように金属イオンを取り込み成熟化されるか Invited

    渡部 聡

    宮崎大学構造生物セミナー  2015.3 

     More details

    Language:Japanese  

    Country:Other  

  • Structural basis of pH- and zinc-dependent multiple client recognition by ERp44 Invited

    Satoshi Watanabe, Yuta Amagai, Roberto Sitia, Kenji Inaba

    2018.9 

     More details

    Language:English  

    Country:Other  

  • 新生金属タンパク質の成熟化の構造基盤

    渡部 聡

    「新生鎖の生物学」第5回若手ワークショップ  2018.5 

     More details

    Language:Japanese  

    Country:Other  

  • Structural study of Hyp protein complexes for the maturation of [NiFe] hydrogenase from Thermococcus kodakarensis International conference

    Watanabe S, Kawashima T, Nishitani Y, Kanai T, Atomi H, Imanaka T, Miki K

    11th International Congress on Extremophiles  2016.9 

     More details

    Language:English  

    Country:Other  

    Structural study of Hyp protein complexes for the maturation of [NiFe] hydrogenase from Thermococcus kodakarensis

▼display all

MISC

  • T細胞受容体(TCR)/CD3複合体を標的とした超硫黄分子による免疫応答制御

    守田 匡伸, 山田 充啓, 佐々木 優作, 井田 智章, 松永 哲郎, 高田 剛, 渡部 聡, 稲葉 謙次, 石井 直人, 杉浦 久敏, 本橋 ほづみ, 赤池 孝章

    日本生化学会大会プログラム・講演要旨集   2021.11

     More details

    Language:Japanese  

  • [NiFe]ヒドロゲナーゼ成熟化の構造生物学

    渡部 聡, 三木 邦夫

    日本結晶学会誌   2020.5

     More details

    Language:Japanese  

    <p>Approximately one-half of all proteins in cells require specific metal ions for proper function, which are delivered by specific auxiliary proteins called metallochaperones. Hydrogen producing enzymes, [NiFe] hydrogenases harbor a complex metal cofactor, NiFe(CN)2CO, in their active sites. Assembly and incorporation of the NiFe(CN)2CO center requires specific maturation machinery, in which six Hyp proteins (HypABCDEF) play key roles. Four Hyp proteins (HypC-HypF) are involved in the biosynthesis and incorporation of the Fe(CN)2CO group. After Fe insertion, HypA and HypB insert the Ni ion into the hydrogenase large subunit. Here, we review recent structural studies of Hyp protein complexes for [NiFe] hydrogenase maturation.</p>

    DOI: 10.5940/jcrsj.62.91

  • 初期分泌経路における新たなタンパク質品質管理機構 ---亜鉛イオンとERp44の協奏

    天貝佑太, 渡部聡, 稲葉謙次

    実験医学   2020.3

     More details

    Language:Japanese  

  • Molecular basis of zinc-dependent regulation of ERp44 for protein quality control in the early secretory pathway Reviewed

    Watanabe S, Inaka K

    Photon factory highlights 2018   2019.4

     More details

    Language:English  

    Molecular basis of zinc-dependent regulation of ERp44 for protein quality control in the early secretory pathway

  • Crystal Structure of the HypA-HypB Complex for [NiFe] Hydrogenase Maturation Reviewed

    Watanabe S, Nishitani Y, Miki K

    Photon factory highlights 2015   2016.4

     More details

    Language:Others  

    Crystal Structure of the HypA-HypB Complex for [NiFe] Hydrogenase Maturation

  • [NiFe]ヒドロゲナーゼへNiを組み込むHypAB複合体の結晶構造解析

    河島拓未, 渡部聡, 西谷優一, 金井保, 和田健彦, 稲葉謙次, 跡見晴幸, 今中忠行, 三木邦夫

    日本蛋白質科学会年会プログラム・要旨集   2015.5

     More details

    Language:Japanese  

  • [NiFe]ヒドロゲナーゼ成熟化おけるシアノ基生合成中間体の構造解析

    渡部聡, 富永大河, 松見理恵, 跡見晴幸, 今中忠行, 三木邦夫

    日本蛋白質科学会年会プログラム・要旨集   2014.5

     More details

    Language:Japanese  

  • 酸化ストレス応答とタンパク質成熟化に関する結晶学的研究 Reviewed

    渡部 聡

    日本結晶学会誌   2012.8

     More details

    Language:Others  

  • Crystal Structure of the Oxidative-Stress Sensor SoxR Bound to DNA Reviewed

    Watanabe S, Miki K

    SPring-8 Research Frontiers 2008   2009.4

     More details

    Language:Others  

    Crystal Structure of the Oxidative-Stress Sensor SoxR Bound to DNA

  • [NiFe]ヒドロゲナーゼ成熟化因子群の構造生物学

    渡部 聡, 三木 邦夫

    化学と生物   2008.9

     More details

    Language:Japanese  

    [NiFe]ヒドロゲナーゼは,水素分子の可逆的な酸化還元反応を触媒する酵素で,活性部位にはシアノ基と一酸化炭素基を配位子にもつNiとFe原子の金属クラスターがある.この複雑な金属クラスターは,生体内で自発的につくられるのではなく,成熟化因子と呼ばれる特異的なタンパク質,Hypタンパク質群の助けによって,段階的に組み込まれて生合成される.最近,Hypタンパク質の立体構造が相次いで決定されて,[NiFe]ヒドロゲナーゼ成熟化機構についての構造生物学的研究は,新しい進展を見せている.

    DOI: 10.1271/kagakutoseibutsu.46.608

  • Crystal structure of atypical cytoplasmic ABC-ATPase SufC.

    S. Watanabe, A. Kita, K. Miki

    XX congress of the International Union of Crystallography, Congress and General Assembly   2005.8

     More details

    Language:Others  

▼display all

Professional Memberships

  • THE JAPANESE BIOCHEMICAL SOCIETY

  • THE BIOPHYSICAL SOCIETY OF JAPAN

  • THE CRYSTALLOGRAPHIC SOCIETY OF JAPAN

  • PROTEIN SCIENCE SOCIETY OF JAPAN

Academic Activities

  • 企画立案・運営等

    第2回東北大学クライオ電子顕微鏡コース(INGEM)(講師・運営)  2023.10

     More details

    Type:Competition, symposium, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2023

     More details

    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:3

  • 企画立案・運営等

    第1回東北大学クライオ電子顕微鏡コース(INGEM)(講師・運営)  2022.10

     More details

    Type:Competition, symposium, etc. 

Research Projects

  • カーゴ受容体群による分泌糖タンパク質の品質管理機構の構造基盤

    2024.4 - 2027.3

      More details

    Authorship:Principal investigator 

  • カーゴ受容体群による分泌糖タンパク質の品質管理機構の構造基盤

    Grant number:24K01960  2024 - 2026

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      More details

    Grant type:Scientific research funding

  • クライオ電子顕微鏡による「柔らかい」一回膜貫通タンパク質の可視化

    2023.6 - 2026.3

      More details

    Authorship:Principal investigator 

  • クライオ電子顕微鏡による「柔らかい」一回膜貫通タンパク質の可視化

    Grant number:23K18193  2023 - 2025

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 上原記念生命科学財団 研究助成金

    2023

      More details

    Grant type:Donation

  • クロススケール細胞内分子構造動態解析が解明する小胞体恒常性維持機構

    2021.9 - 2026.3

      More details

    Authorship:Coinvestigator(s) 

  • クロススケール細胞内分子構造動態解析が解明する小胞体恒常性維持機構

    Grant number:21H05253  2021 - 2025

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(A)

      More details

    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • クライオ電顕を用いたNaチャネルの構造解析による鎮痛特異的な局所麻酔薬の開発

    Grant number:21K19517  2021 - 2023

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

      More details

    Grant type:Scientific research funding

  • 持田記念医学薬学振興財団

    2021

      More details

    Grant type:Donation

  • 武田科学振興財団ライフサイエンス研究助成

    2020

      More details

    Grant type:Donation

  • 免疫グロブリンMの成熟化に関与するシャペロン複合体の分子機構の解明

    2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • 内藤記念科学奨励金・研究助成

    2018

      More details

    Grant type:Donation

  • ヒドロゲナーゼ成熟化の分子機構に関する構造生物学研究

    Grant number:17H03642  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      More details

    Grant type:Scientific research funding

  • 巨大カルシウムチャネルであるリアノジン受容体の構造と制御機構の解明

    2015 - 2016

    科学研究費助成事業  若手研究(A,B)

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

  • ヒドロゲナーゼ成熟化における金属クラスター組み込みの分子機構

    Grant number:26291012  2014 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      More details

    Grant type:Scientific research funding

  • 上原記念生命科学財団 研究奨励金

    2013

      More details

    Grant type:Donation

▼display all

Class subject

  • システム生命学府生命医科学特論I

    2024.4 - 2024.9   First semester