Updated on 2025/01/09

Information

 

写真a

 
HARADA AKIHITO
 
Organization
Faculty of Medical Sciences Department of Advanced Medical Initiatives Professor
Graduate School of Systems Life Sciences Department of Systems Life Sciences(Concurrent)
Graduate School of Medical Sciences Department of Medical Sciences(Concurrent)
Title
Professor
Contact information
メールアドレス
Tel
0926424534
External link

Degree

  • Agriculture

Research Interests・Research Keywords

  • Research theme:Understanding the Mechanism of Epigenetic Marking by Histone Variants in Germ Cells

    Keyword:germ cell, cell differentiation, epigenetics, histone variant, transcript

    Research period: 2016.4

  • Research theme:Understanding the Mechanism of Cell Fate Determination Modeled by Skeletal Muscle Differentiation

    Keyword:skeletal muscle, cell differentiation, epigenetics, histone variant, transcript

    Research period: 2016.4

  • Research theme:Understanding the Mechanism of Selective Gene Expression of the Novel Histone Variant H3mm7 in Skeletal Muscle Differentiation

    Keyword:skeletal muscle, cell differentiation, epigenetics, histone variant, transcript

    Research period: 2015.4

Awards

  • 公益財団法人福岡県すこやか健康事業団平成28年度がん研究助成金優秀賞

    2017.1   公益財団法人福岡県すこやか健康事業団   発がんを誘発するヒストンH3バリアントの変異部位の同定とその誘発メカニズムを明らかにすることを目的としエピジェネティック破綻により引き起こされた筋肉腫の解析を進める。具体的には、横紋筋肉腫をモデルとし、正常組織とがん組織における各H3バリアントのゲノム領域への取り込み、またそれらが形成するクロマチン構造を明らかにし、ヒストンバリアントの破綻によるがん化メカニズムを解明する。

Papers

  • Chromatin integration labeling for mapping DNA-binding proteins and modifications with low input. Reviewed International journal

    Nature Protocols   15 ( 10 )   3334 - 3360   2020.10

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41596-020-0375-8.

  • A chromatin integration labelling method enables epigenomic profiling with lower input Reviewed

    Akihito Harada, Kazumitsu Maehara, Tetsuya Handa, Yasuhiro Arimura, Jumpei Nogami, Yoko Hayashi-Takanaka, Katsuhiko Shirahige, Hitoshi Kurumizaka, Hiroshi Kimura, Yasuyuki Ohkawa

    Nature Cell Biology   21 ( 2 )   287 - 296   2019.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41556-018-0248-3

  • Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration Reviewed

    Akihito Harada, Kazumitsu Maehara, Yusuke Ono, Hiroyuki Taguchi, Kiyoshi Yoshioka, Yasuo Kitajima, Yan Xie, Yuko Sato, Takeshi Iwasaki, Jumpei Nogami, Seiji Okada, Tetsuro Komatsu, Yuichiro Semba, Tatsuya Takemoto, Hiroshi Kimura, Hitoshi Kurumizaka, Yasuyuki Ohkawa

    Nature Communications   9 ( 1 )   2018.12

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-03845-1

  • Temporal regulation of chromatin during myoblast differentiation Reviewed

    Akihito Harada, Yasuyuki Ohkawa, Anthony N. Imbalzano

    Seminars in Cell and Developmental Biology   72   77 - 86   2017.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.semcdb.2017.10.022

  • Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis Reviewed

    Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata

    Cell Reports   18 ( 3 )   593 - 600   2017.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2016.12.065

  • Tissue-specific expression of histone H3 variants diversified after species separation Reviewed

    Kazumitsu Maehara, Akihito Harada, Yuko Sato, Masaki Matsumoto, Keiichi Nakayama, Hiroshi Kimura, Yasuyuki Ohkawa

    Epigenetics and Chromatin   8 ( 1 )   2015.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s13072-015-0027-3

  • Spatial re-organization of myogenic regulatory sequences temporally controls gene expression Reviewed International coauthorship

    Akihito Harada, Chandrashekara Mallappa, Seiji Okada, John T. Butler, Stephen P. Baker, Jeanne B. Lawrence, Yasuyuki Ohkawa, Anthony N. Imbalzano

    Nucleic Acids Research   43 ( 4 )   2008 - 2021   2015.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gkv046

  • Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle Reviewed

    Akihito Harada, Kazumitsu Maehara, Yuko Sato, Daijiro Konno, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa

    Nucleic Acids Research   43 ( 2 )   775 - 786   2015.1

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    DOI: 10.1093/nar/gku1346

  • Chd2 interacts with H3.3 to determine myogenic cell fate Reviewed

    Akihito Harada, Seiji Okada, Daijiro Konno, Jun Odawara, Tomohiko Yoshimi, Saori Yoshimura, Hiromi Kumamaru, Hirokazu Saiwai, Toshiaki Tsubota, Hitoshi Kurumizaka, Koichi Akashi, Taro Tachibana, Anthony N. Imbalzano, Yasuyuki Ohkawa

    EMBO Journal   31 ( 13 )   2994 - 3007   2012.7

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/emboj.2012.136

  • The anti-tumor effect of trifluridine via induction of aberrant mitosis is unaffected by mutations modulating p53 activity

    Wakasa, T; Nonaka, K; Harada, A; Ohkawa, Y; Kikutake, C; Suyama, M; Kobunai, T; Tsunekuni, K; Matsuoka, K; Kataoka, Y; Ochiiwa, H; Miyadera, K; Sagara, T; Oki, E; Ohdo, S; Maehara, Y; Iimori, M; Kitao, H

    CELL DEATH DISCOVERY   10 ( 1 )   307   2024.7   ISSN:2058-7716 eISSN:2058-7716

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    Language:English   Publisher:Cell Death Discovery  

    The fluorinated thymidine analog trifluridine (FTD) is a chemotherapeutic drug commonly used to treat cancer; however, the mechanism by which FTD induces cytotoxicity is not fully understood. In addition, the effect of gain-of-function (GOF) missense mutations of the TP53 gene (encoding p53), which promote cancer progression and chemotherapeutic drug resistance, on the chemotherapeutic efficacy of FTD is unclear. Here, we revealed the mechanisms by which FTD-induced aberrant mitosis and contributed to cytotoxicity in both p53-null and p53-GOF missense mutant cells. In p53-null mutant cells, FTD-induced DNA double-stranded breaks, single-stranded DNA accumulation, and the associated DNA damage responses during the G2 phase. Nevertheless, FTD-induced DNA damage and the related responses were not sufficient to trigger strict G2/M checkpoint arrest. Thus, these features were carried over into mitosis, resulting in chromosome breaks and bridges, and subsequent cytokinesis failure. Improper mitotic exit eventually led to cell apoptosis, caused by the accumulation of extensive DNA damage and the presence of micronuclei encapsulated in the disrupted nuclear envelope. Upon FTD treatment, the behavior of the p53-GOF-missense mutant, isogenic cell lines, generated by CRISPR/Cas9 genome editing, was similar to that of p53-null mutant cells. Thus, our data suggest that FTD treatment overrode the effect on gene expression induced by p53-GOF mutants and exerted its anti-tumor activity in a manner that was independent of the p53 function.

    DOI: 10.1038/s41420-024-02083-3

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  • Plasma cell differentiation is regulated by the expression of histone variant H3.3. International journal

    Yuichi Saito, Akihito Harada, Miho Ushijima, Kaori Tanaka, Ryota Higuchi, Akemi Baba, Daisuke Murakami, Stephen L Nutt, Takashi Nakagawa, Yasuyuki Ohkawa, Yoshihiro Baba

    Nature communications   15 ( 1 )   5004 - 5004   2024.6   eISSN:2041-1723

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    The differentiation of B cells into plasma cells is associated with substantial transcriptional and epigenetic remodeling. H3.3 histone variant marks active chromatin via replication-independent nucleosome assembly. However, its role in plasma cell development remains elusive. Herein, we show that during plasma cell differentiation, H3.3 is downregulated, and the deposition of H3.3 and chromatin accessibility are dynamically changed. Blockade of H3.3 downregulation by enforced H3.3 expression impairs plasma cell differentiation in an H3.3-specific sequence-dependent manner. Mechanistically, enforced H3.3 expression inhibits the upregulation of plasma cell-associated genes such as Irf4, Prdm1, and Xbp1 and maintains the expression of B cell-associated genes, Pax5, Bach2, and Bcl6. Concomitantly, sustained H3.3 expression prevents the structure of chromatin accessibility characteristic for plasma cells. Our findings suggest that appropriate H3.3 expression and deposition control plasma cell differentiation.

    DOI: 10.1038/s41467-024-49375-x

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  • Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states Reviewed

    Tomimatsu, K; Fujii, T; Bise, R; Hosoda, K; Taniguchi, Y; Ochiai, H; Ohishi, H; Ando, K; Minami, R; Tanaka, K; Tachibana, T; Mori, S; Harada, A; Maehara, K; Nagasaki, M; Uchida, S; Kimura, H; Narita, M; Ohkawa, Y

    NATURE COMMUNICATIONS   15 ( 1 )   3657   2024.5   eISSN:2041-1723

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    Language:English   Publisher:Nature Communications  

    Cell states are regulated by the response of signaling pathways to receptor ligand-binding and intercellular interactions. High-resolution imaging has been attempted to explore the dynamics of these processes and, recently, multiplexed imaging has profiled cell states by achieving a comprehensive acquisition of spatial protein information from cells. However, the specificity of antibodies is still compromised when visualizing activated signals. Here, we develop Precise Emission Canceling Antibodies (PECAbs) that have cleavable fluorescent labeling. PECAbs enable high-specificity sequential imaging using hundreds of antibodies, allowing for reconstruction of the spatiotemporal dynamics of signaling pathways. Additionally, combining this approach with seq-smFISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system can serve as a comprehensive platform for analyzing complex cell processes.

    DOI: 10.1038/s41467-024-47989-9

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  • Production of a Monoclonal Antibody for Histone H2b Isoform H2b3b. Reviewed International journal

    Saki Egashira, Taro Tachibana, Mako Nakamura, Yasuyuki Ohkawa, Akihito Harada

    Monoclonal antibodies in immunodiagnosis and immunotherapy   43 ( 2 )   75 - 80   2024.4

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    H2b3b is one of the histone H2b isoforms that differs from canonical H2b by five to six amino acids. Previously, we identified H3t as the testis-specific histone H3 variant located in histone cluster 3, which is also the site of H2b3b. In this study, we produced monoclonal antibodies against H2b3b, using the iliac rat lymph node method for rat antibody and the immunochamber method for rabbit antibody. Immunoblot analysis confirmed that our antibodies could specifically discriminate between H2b3b and canonical H2b. Moreover, immunostaining revealed colocalization with a testicular stem cell marker, Plzf, but not with a meiotic marker, Sycp. This indicated that H2b3b is expressed in spermatogenic cells before meiosis. Our monoclonal antibodies enable further studies to reveal specific functions of H2b3b during spermatogenesis. We also hope that the established method will lead to the production of antibodies that can identify other H2b isoforms.

    DOI: 10.1089/mab.2023.0025

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  • Monoclonal Antibody Rat 2F11 and Rabbit A3 Against Anti-H2b3b. Reviewed International journal

    Saki Egashira, Taro Tachibana, Mako Nakamura, Yasuyuki Ohkawa, Akihito Harada

    Monoclonal antibodies in immunodiagnosis and immunotherapy   43 ( 2 )   81 - 82   2024.4

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    DOI: 10.1089/mab.2024.0005

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  • 特集 クロマチンによる転写制御機構の最前線 Ⅴ.クロマチンと転写制御に関する新技術 クロマチン構造における転写制御の理解に向けた技術開発

    原田 哲仁, 富松 航佑, 武 千湄, 大川 恭行

    生体の科学   74 ( 3 )   255 - 259   2023.6   ISSN:03709531 eISSN:18835503

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    Publisher:株式会社医学書院  

    DOI: 10.11477/mf.2425201685

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  • FOXK1 promotes nonalcoholic fatty liver disease by mediating mTORC1-dependent inhibition of hepatic fatty acid oxidation Reviewed International journal

    Shun Fujinuma, Hirokazu Nakatsumi, Hideyuki Shimizu, Shigeaki Sugiyama, Akihito Harada, Takeshi Goya, Masatake Tanaka, Motoyuki Kohjima, Masatomo Takahashi, Yoshihiro Izumi, Mikako Yagi, Dongchon Kang, Mari Kaneko, Mayo Shigeta, Takeshi Bamba, Yasuyuki Ohkawa, Keiichi I. Nakayama

    Cell Reports   42 ( 5 )   112530 - 112530   2023.5   ISSN:2211-1247

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    Language:Others   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.celrep.2023.112530

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  • Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections. Reviewed International journal

    Mizuki Honda, Ryuichi Kimura, Akihito Harada, Kazumitsu Maehara, Kaori Tanaka, Yasuyuki Ohkawa, Shinya Oki

    STAR protocols   3 ( 2 )   101346 - 101346   2022.6   ISSN:2666-1667

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    Photo-isolation chemistry (PIC) enables isolation of transcriptome information from locally defined areas by photo-irradiation. Here, we present an optimized PIC protocol for formalin-fixed frozen and paraffin mouse sections and fresh-frozen mouse sections. We describe tissue section preparation and permeabilization, followed by in situ reverse transcription using photo-caged primers. We then detail immunostaining and UV-mediated uncaging to the target areas, followed by linear amplification of uncaged cDNAs, library preparation, and quantification. This protocol can be applied to various animal tissue types. For complete details on the use and execution of this protocol, please refer to Honda et al. (2021).

    DOI: 10.1016/j.xpro.2022.101346

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  • Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail. Reviewed International journal

    Satoshi Ishishita, Shumpei Kitahara, Mayuko Takahashi, Sakura Iwasaki, Shoji Tatsumoto, Izumi Hara, Yoshiki Kaneko, Keiji Kinoshita, Katsushi Yamaguchi, Akihito Harada, Yasushige Ohmori, Yasuyuki Ohkawa, Yasuhiro Go, Shuji Shigenobu, Yoichi Matsuda, Takayuki Suzuki

    PloS one   17 ( 3 )   e0265008   2022.3   ISSN:1932-6203

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    The precursor of heme, protoporphyrin IX (PPIX), accumulates abundantly in the uteri of birds, such as Japanese quail, Coturnix japonica, which has brown-speckled eggshells; however, the molecular basis of PPIX production in the uterus remains largely unknown. Here, we investigated the cause of low PPIX production in a classical Japanese quail mutant exhibiting white eggshells by comparing its gene expression in the uterus with that of the wild type using transcriptome analysis. We also performed genetic linkage analysis to identify the causative genomic region of the white eggshell phenotype. We found that 11 genes, including 5'-aminolevulinate synthase 1 (ALAS1) and hephaestin-like 1 (HEPHL1), were specifically upregulated in the wild-type uterus and downregulated in the mutant. We mapped the 172 kb candidate genomic region on chromosome 6, which contains several genes, including a part of the paired-like homeodomain 3 (PITX3), which encodes a transcription factor. ALAS1, HEPHL1, and PITX3 were expressed in the apical cells of the luminal epithelium and lamina propria cells of the uterine mucosa of the wild-type quail, while their expression levels were downregulated in the cells of the mutant quail. Biochemical analysis using uterine homogenates indicated that the restricted availability of 5'-aminolevulinic acid is the main cause of low PPIX production. These results suggest that uterus-specific transcriptional regulation of heme-biosynthesis-related genes is an evolutionarily acquired mechanism of eggshell pigment production in Japanese quail. Based on these findings, we discussed the molecular basis of PPIX production in the uteri of Japanese quails.

    DOI: 10.1371/journal.pone.0265008

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  • High-depth spatial transcriptome analysis by photo-isolation chemistry Reviewed International journal

    Mizuki Honda, Shinya Oki, Ryuichi Kimura, Akihito Harada, Kazumitsu Maehara, Kaori Tanaka, Chikara Meno, Yasuyuki Ohkawa

    Nature Communications   12 ( 1 )   2021.12

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    DOI: 10.1038/s41467-021-24691-8

  • Recent advances in single-cell epigenomics. Reviewed International journal

    Akihito Harada, Hiroshi Kimura, Yasuyuki Ohkawa

    Current opinion in structural biology   71   116 - 122   2021.12

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    DOI: 10.1016/j.sbi.2021.06.010

  • Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay. Reviewed International journal

    Hiroaki Tachiwana, Mariko Dacher, Kazumitsu Maehara, Akihito Harada, Yosuke Seto, Ryohei Katayama, Yasuyuki Ohkawa, Hiroshi Kimura, Hitoshi Kurumizaka, Noriko Saitoh

    eLife   10   2021.5

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    DOI: 10.7554/eLife.66290

  • Transcriptome analysis of gene expression changes upon enzymatic dissociation in skeletal myoblasts. Reviewed International journal

    Atsuko Miyawaki-Kuwakado, Qianmei Wu, Akihito Harada, Kosuke Tomimatsu, Takeru Fujii, Kazumitsu Maehara, Yasuyuki Ohkawa

    Genes to cells : devoted to molecular & cellular mechanisms   2021.5

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    DOI: 10.1111/gtc.12870

  • Targeted inhibition of EPAS1-driven IL-31 production by a small-molecule compound Reviewed International journal

    Yasuhisa Kamikaseda, Takehito Uruno, Kazufumi Kunimura, Akihito Harada, Kuniko Saiki, Kounosuke Oisaki, Daiji Sakata, Takeshi Nakahara, Makiko Kido-Nakahara, Motomu Kanai, Seiji Nakamura, Yasuyuki Ohkawa, Masutaka Furue, Yoshinori Fukui.

    The Journal of Allergy and Clinical Immunology   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jaci.2021.03.029

  • Genome-wide analysis of chromatin structure changes upon MyoD binding in proliferative myoblasts during the cell cycle. Reviewed International journal

    Qianmei Wu, Takeru Fujii, Akihito Harada, Kosuke Tomimatsu, Atsuko Miyawaki-Kuwakado, Masatoshi Fujita, Kazumitsu Maehara, Yasuyuki Ohkawa

    Journal of biochemistry   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvab001

  • Modeling population size independent tissue epigenomes by ChIL-seq with single-thin sections Reviewed International journal

    Kazumitsu Maehara, Kosuke Tomimatsu, Akihito Harada, Kaori Tanaka, Shoko Sato, Seiji Okada, Tetsuya Handa, Hitoshi Kurumizaka, Hiroshi Kimura, Yasuyuki Ohkawa

    Molecular Systems Biology   2020.12

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    DOI: 10.1101/2020.12.18.423434

  • Subnuclear gene positioning through lamina association affects copper tolerance Reviewed International journal

    Yuki Sakamoto, Mayuko Sato, Yoshikatsu Sato, Akihito Harada, Takamasa Suzuki, Chieko Goto, Kentaro Tamura, Kiminori Toyooka, Hiroshi Kimura, Yasuyuki Ohkawa, Ikuko Hara-Nishimura, Shingo Takagi, Sachihiro Matsunaga

    Nature Communications   11 ( 1 )   2020.12

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    DOI: 10.1038/s41467-020-19621-z

  • Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Reviewed International journal

    Hiroshi Ochiai, Tetsutaro Hayashi, Mana Umeda, Mika Yoshimura, Akihito Harada, Yukiko Shimizu, Kenta Nakano, Noriko Saitoh, Zhe Liu, Takashi Yamamoto, Tadashi Okamura, Yasuyuki Ohkawa, Hiroshi Kimura, Itoshi Nikaido

    Science advances   6 ( 25 )   eaaz6699   2020.6

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    DOI: 10.1126/sciadv.aaz6699

  • Chromatin-bound CRM1 recruits SET-Nup214 and NPM1c onto HOX clusters causing aberrant HOX expression in leukemia cells Reviewed

    Masahiro Oka, Sonoko Mura, Mayumi Otani, Yoichi Miyamoto, Jumpei Nogami, Kazumitsu Maehara, Akihito Harada, Taro Tachibana, Yoshihiro Yoneda, Yasuyuki Ohkawa

    eLife   8   2019.11

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    DOI: 10.7554/eLife.46667

  • Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle Reviewed

    Sumiaki Fukuda, Akihiro Kaneshige, Takayuki Kaji, Yu Taro Noguchi, Yusei Takemoto, Lidan Zhang, Kazutake Tsujikawa, Hiroki Kokubo, Akiyoshi Uezumi, Kazumitsu Maehara, Akihito Harada, Yasuyuki Ohkawa, So Ichiro Fukada

    eLife   8   2019.9

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    DOI: 10.7554/eLife.48284

  • Biochemical analysis of nucleosome targeting by Tn5 transposase Reviewed

    Shoko Sato, Yasuhiro Arimura, Tomoya Kujirai, Akihito Harada, Kazumitsu Maehara, Jumpei Nogami, Yasuyuki Ohkawa, Hitoshi Kurumizaka

    Open Biology   9 ( 8 )   2019.8

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    DOI: 10.1098/rsob.190116

  • Cell-autonomous and redundant roles of Hey1 and HeyL in muscle stem cells HeyL requires HeS1 to bind diverse DNA sites Reviewed

    Yu Taro Noguchi, Miki Nakamura, Nobumasa Hino, Jumpei Nogami, Sayaka Tsuji, Takahiko Sato, Lidan Zhang, Kazutake Tsujikawa, Toru Tanaka, Kohei Izawa, Yoshiaki Okada, Takefumi Doi, Hiroki Kokubo, Akihito Harada, Akiyoshi Uezumi, Manfred Gessler, Yasuyuki Ohkawa, So Ichiro Fukada

    Development (Cambridge)   146 ( 4 )   2019.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/dev.163618

  • Locomotor Training Increases Synaptic Structure With High NGL-2 Expression After Spinal Cord Hemisection Reviewed

    Kazu Kobayakawa, Kyleigh Alexis DePetro, Hui Zhong, Bau Pham, Masamitsu Hara, Akihito Harada, Jumpei Nogami, Yasuyuki Ohkawa, V. Reggie Edgerton

    Neurorehabilitation and Neural Repair   2019.1

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    DOI: 10.1177/1545968319829456

  • Roles of histone H3.5 in human spermatogenesis and spermatogenic disorders Reviewed

    K. Shiraishi, A. Shindo, Akihito Harada, H. Kurumizaka, H. Kimura, Yasuyuki Ohkawa, H. Matsuyama

    Andrology   6 ( 1 )   158 - 165   2018.1

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    DOI: 10.1111/andr.12438

  • Sensitive detection of fluorescence in western blotting by merging images Reviewed

    Yukari Kondo, Shinichiro Higa, Takeshi Iwasaki, Tomoya Matsumoto, Kazumitsu Maehara, Akihito Harada, Yoshihiro Baba, Masatoshi Fujita, Yasuyuki Ohkawa

    PLoS One   13 ( 1 )   2018.1

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    DOI: 10.1371/journal.pone.0191532

  • Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells Reviewed

    Yuichiro Semba, Akihito Harada, Kazumitsu Maehara, Shinya Oki, Chikara Meno, Jun Ueda, Kazuo Yamagata, Atsushi Suzuki, Mitsuho Onimaru, Jumpei Nogami, Seiji Okada, Koichi Akashi, Yasuyuki Ohkawa

    Nucleic Acids Research   45 ( 15 )   8758 - 8772   2017.9

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    DOI: 10.1093/nar/gkx475

  • Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.8 Reviewed

    Hiroyuki Taguchi, Yan Xie, Naoki Horikoshi, Kazumitsu Maehara, Akihito Harada, Jumpei Nogami, Koichi Sato, Yasuhiro Arimura, Akihisa Osakabe, Tomoya Kujirai, Takeshi Iwasaki, Yuichiro Semba, Taro Tachibana, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka

    Biochemistry   56 ( 16 )   2184 - 2196   2017.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.biochem.6b01098

  • ROLES OF HISTONE H3.5 IN HUMAN SPERMATOGENESIS AND SPERMATOGENIC DISORDERS Reviewed

    Koji Shiraishi, Aya Shindo, Akihito Harada, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Hiroshi Kimura, Hideyasu Matsuyama

    JOURNAL OF UROLOGY   197 ( 4 )   E85 - E85   2017.4

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    DOI: 10.1016/j.juro.2017.02.277

  • Periostin Promotes Scar Formation through the Interaction between Pericytes and Infiltrating Monocytes/Macrophages after Spinal Cord Injury Reviewed

    Kazuya Yokota, Kazu Kobayakawa, Takeyuki Saito, Masamitsu Hara, Ken Kijima, Yasuyuki Ohkawa, Akihito Harada, Ken Okazaki, Kohei Ishihara, Shigeo Yoshida, Akira Kudo, Yukihide Iwamoto, Seiji Okada

    American Journal of Pathology   187 ( 3 )   639 - 653   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ajpath.2016.11.010

  • The requirement of Mettl3-promoted MyoD mRNA maintenance in proliferative myoblasts for skeletal muscle differentiation Reviewed

    Kensuke Kudou, Tetsuro Komatsu, Jumpei Nogami, Kazumitsu Maehara, Akihito Harada, Hiroshi Saeki, Eiji Oki, Yoshihiko Maehara, Yasuyuki Ohkawa

    Open Biology   7 ( 9 )   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1098/rsob.170119

  • Identification of immunoglobulin gene sequences from a small read number of mRNA-seq using hybridomas Reviewed

    Yuki Kuniyoshi, Kazumitsu Maehara, Takeshi Iwasaki, Masayasu Hayashi, Yuichiro Semba, Masatoshi Fujita, Yuko Sato, Hiroshi Kimura, Akihito Harada, Yasuyuki Ohkawa

    PLoS One   11 ( 10 )   2016.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0165473

  • Histone H4 lysine 20 acetylation is associated with gene repression in human cells Reviewed

    Jun Ya Kaimori, Kazumitsu Maehara, Yoko Hayashi-Takanaka, Akihito Harada, Masafumi Fukuda, Satoko Yamamoto, Naotsugu Ichimaru, Takashi Umehara, Shigeyuki Yokoyama, Ryo Matsuda, Tsuyoshi Ikura, Koji Nagao, Chikashi Obuse, Naohito Nozaki, Shiro Takahara, Toshifumi Takao, Yasuyuki Ohkawa, Hiroshi Kimura, Yoshitaka Isaka

    Scientific Reports   6   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep24318

  • Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2 Reviewed

    Masayasu Hayashi, Kazumitsu Maehara, Akihito Harada, Yuichiro Semba, Kensuke Kudo, Hidehisa Takahashi, Shinya Oki, Chikara Meno, Kenji Ichiyanagi, Koichi Akashi, Yasuyuki Ohkawa

    Journal of Cellular Biochemistry   117 ( 3 )   780 - 792   2016.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jcb.25368

  • Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis Reviewed

    Takashi Urahama, Akihito Harada, Kazumitsu Maehara, Naoki Horikoshi, Koichi Sato, Yuko Sato, Koji Shiraishi, Norihiro Sugino, Akihisa Osakabe, Hiroaki Tachiwana, Wataru Kagawa, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka

    Epigenetics and Chromatin   9 ( 1 )   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s13072-016-0051-y

  • Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies Reviewed

    Yoko Hayashi-Takanaka, Kazumitsu Maehara, Akihito Harada, Takashi Umehara, Shigeyuki Yokoyama, Chikashi Obuse, Yasuyuki Ohkawa, Naohito Nozaki, Hiroshi Kimura

    Chromosome Research   23 ( 4 )   753 - 766   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10577-015-9486-4

  • Relationship between the risk for a shrimp allergy and freshness or cooking Reviewed

    Masakatsu Usui, Akihito Harada, Shinya Yasumoto, Yoshimasa Sugiura, Anri Nishidai, Maria Ikarashi, Honami Takaba, Taiko Miyasaki, Hiroyuki Azakami, Masakazu Kondo

    Bioscience, Biotechnology and Biochemistry   79 ( 10 )   1698 - 1701   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/09168451.2015.1045830

  • Establishment of neutralizing rat monoclonal antibodies for fibroblast growth factor-2 Reviewed

    Masako Tanaka, Maki Yamaguchi, Masayuki Shiota, Yukiko Kawamoto, Katsuyuki Takahashi, Azusa Inagaki, Mayuko Osada-Oka, Akihito Harada, Hideki Wanibuchi, Yasukatsu Izumi, Katsuyuki Miura, Hiroshi Iwao, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 4 )   261 - 269   2014.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/mab.2013.0085

  • Hsc70 contributes to cancer cell survival by preventing Rab1A degradation under stress conditions Reviewed

    Masako Tanaka, Saya Mun, Akihito Harada, Yasuyuki Ohkawa, Azusa Inagaki, Soichi Sano, Katsuyuki Takahashi, Yasukatsu Izumi, Mayuko Osada-Oka, Hideki Wanibuchi, Masayo Yamagata, Tokihito Yukimura, Katsuyuki Miura, Masayuki Shiota, Hiroshi Iwao

    PLoS One   9 ( 5 )   2014.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0096785

  • Generation of a monoclonal antibody for INI1/hSNF5/BAF47 Reviewed

    Akihito Harada, Masayasu Hayashi, Yuuki Kuniyoshi, Yuichiro Semba, Satoko Sugahara, Taro Tachibana, Yasuyuki Ohkawa, Masatoshi Fujita

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33 ( 1 )   49 - 51   2014.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/mab.2013.0065

  • Production of a monoclonal antibody for C/EBPβ The subnuclear localization of C/EBPβ in mouse L929 cells Reviewed

    33 ( 1 )   34 - 37   2014.2

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    The CCAAT/enhancer-binding protein (C/EBP)β belongs to the C/EBP family of proteins that possesses a basic leucine zipper DNA-binding domain. These proteins bind DNA by dimerization and play a role in the transcriptional regulation of various cells. There are six different types of C/EBPs, and some form isoforms through the use of alternative translation initiation sites. The functional analysis of the C/EBP family is therefore difficult to achieve. Here we report on the production of specific monoclonal antibodies against mouse C/EBPβ using a rat medial iliac lymph node method. Immunoblotting using C/EBPβ monoclonal antibodies identified two types of isoforms, while immunostaining revealed a subnuclear localization for C/EBPβ. Use of this antibody should contribute to the further elucidation of the transcriptional regulatory function of C/EBPβ.

    DOI: 10.1089/mab.2013.0069

  • Contribution of structural reversibility to the heat stability of the tropomyosin shrimp allergen Reviewed

    Masakatsu Usui, Akihito Harada, Takayuki Ishimaru, Emiri Sakumichi, Fumihiko Saratani, Chiho Sato-Minami, Hiroyuki Azakami, Taiko Miyasaki, Ken'ichi Hanaoka

    Bioscience, Biotechnology and Biochemistry   77 ( 5 )   948 - 953   2013.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.120887

  • A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples Reviewed

    Kazumitsu Maehara, Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Koji Nagao, Chikashi Obuse, Koichi Akashi, Taro Tachibana, Toshio Sakata, Yasuyuki Ohkawa

    Nucleic Acids Research   41 ( 1 )   54 - 62   2013.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gks1010

  • Structural reversibility contributes the heat stability of shrimp allergen tropomyosin Reviewed

    M. Usui, A. Harada, T. Ishimaru, E. Sakumichi, F. Saratani, C. Sato, H. Azakami, T. Miyasaki, K. Hanaoka

    Bioscience, Biotechnology, and Biochemistry   77 ( 5 )   2012.5

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  • The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach Reviewed

    Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Kazumitsu Maehara, Taro Tachibana, Seiji Okada, Koichi Akashi, Yasuyuki Ohkawa

    BMC Genomics   12   2011.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1471-2164-12-516

  • Generation of a rat monoclonal antibody specific for Hsp72 Reviewed

    Masako Tanaka, Masayuki Shiota, Seiji Okada, Akihito Harada, Jun Odawara, Saya Mun, Hiroshi Iwao, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   30 ( 4 )   397 - 400   2011.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2011.0015

  • Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue Reviewed

    Seiji Okada, Hirokazu Saiwai, Hiromi Kumamaru, Kensuke Kubota, Akihito Harada, Masahiro Yamaguchi, Yukihide Iwamoto, Yasuyuki Ohkawa

    Journal of Cellular Physiology   226 ( 2 )   552 - 558   2011.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jcp.22365

  • Generation of a rat monoclonal antibody specific for heat shock cognate protein 70 Reviewed

    Masayuki Shiota, Hirokazu Saiwai, Saya Mun, Akihito Harada, Seiji Okada, Jun Odawara, Masako Tanaka, Hiroshi Iwao, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 5 )   453 - 456   2010.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2010.0024

  • Monoclonal antibody specific for Dhx9/NDHII/RHA Reviewed

    Manato Kotani, Akihito Harada, Jun Odawara, Masayuki Azuma, Seiji Okada, Yuko Nishiyama, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 3 )   259 - 261   2010.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0107

  • Rat monoclonal antibody specific for MyoD Reviewed

    Akihito Harada, Yasuyuki Ohkawa, Shinpei Ao, Jun Odawara, Seiji Okada, Masayuki Azuma, Yuko Nishiyama, Mako Nakamura, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 3 )   255 - 258   2010.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0117

  • Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1 Reviewed

    Saori Yoshimura, Akihito Harada, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Yasuyuki Ohkawa, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 3 )   237 - 240   2010.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0106

  • Generation of a rat monoclonal antibody specific for Chd2 Reviewed

    Akihito Harada, Saori Yoshimura, Jun Odawara, Masayuki Azuma, Seiji Okada, Mako Nakamura, Taro Tachibana, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 2 )   173 - 177   2010.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0090

  • Production of a rat monoclonal antibody specific for Myf5 Reviewed

    Akihito Harada, Seiji Okada, Jun Odawara, Hiromi Kumamaru, Hirokazu Saiwai, Mayumi Aoki, Mako Nakamura, Yuko Nishiyama, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   29 ( 1 )   59 - 62   2010.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0066

  • The LTB4-BLT1 axis mediates neutrophil infiltration and secondary injury in experimental spinal cord injury Reviewed

    Hirokazu Saiwai, Yasuyuki Ohkawa, Hisakata Yamada, Hiromi Kumamaru, Akihito Harada, Hideyuki Okano, Takehiko Yokomizo, Yukihide Iwamoto, Seiji Okada

    American Journal of Pathology   176 ( 5 )   2352 - 2366   2010.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2353/ajpath.2010.090839

  • Generation of a rat monoclonal antibody specific for Pax7 Reviewed

    Akihito Harada, Seiji Okada, Hirokazu Saiwai, Mayumi Aoki, Mako Nakamura, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   28 ( 6 )   451 - 453   2009.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0039

  • Production of a rat monoclonal antibody against Brg1 Reviewed

    Yasuyuki Ohkawa, Akihito Harada, Mako Nakamura, Saori Yoshimura, Taro Tachibana

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   28 ( 6 )   463 - 466   2009.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0041

  • Generation of a rat monoclonal antibody specific for Brm Reviewed

    Seiji Okada, Akihito Harada, Hirokazu Saiwai, Mako Nakamura, Yasuyuki Ohkawa

    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   28 ( 6 )   455 - 458   2009.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/hyb.2009.0044

  • Amyloid fibril formation of hen lysozyme depends on the instability of the C-helix (88-99) Reviewed

    Akihito Harada, Hiroyuki Azakami, Akio Kato

    Bioscience, Biotechnology and Biochemistry   72 ( 6 )   1523 - 1530   2008.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.80032

  • Relationship between the stability of hen egg-white lysozymes mutated at sites designed to interact with α-helix dipoles and their secretion amounts in yeast Reviewed

    71 ( 12 )   2952 - 2961   2007.12

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    The positively charged lysine at the C-terminals of three long α-helices (5-15, 25-35, and 88-99) was replaced with alanine (K13A, K33A, K97A) or aspartic acid (K13D, K33D, K97D) in hen lysozyme by genetic engineering. The denaturation transition point (Tm) and Gibbs energy change ΔG of the mutant lysozymes decreased remarkably, suggesting that the positive charge at the C-terminals of helices is involved in the stabilization of the helix dipole. On the other hand, the non-charged asparagine at the N-terminal of the long α-helices (25-35 and 88-99) was replaced with negatively charged aspartic acid (N27D and N93D). The Tm and ΔG of N27D increased, suggesting that the dipole moment of the N-terminal of the helices is diminished by replacement with negatively charged amino acid strengthening the stability of the helices. The stabilities of those hen egg white lysozymes mutated at the N- or Cterminal sites of the three long α-helices were related with their secretion amounts in yeast (Pichia pastoris). The secretion amounts of these mutant lysozymes in yeast were closely correlated with their stability.

    DOI: 10.1271/bbb.70354

  • Characterization of recombinant amyloidogenic chicken cystatin mutant I66Q expressed in yeast Reviewed

    Jianwei He, Youtao Song, Nobuhiro Ueyama, Akihito Harada, Hiroyuki Azakami, Akio Kato

    Journal of Biochemistry   137 ( 4 )   477 - 485   2005.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/jb/mvi064

  • Effect of EPS1 gene deletion in Saccharomyces cerevisiae on the secretion of foreign proteins which have disulfide bridges Reviewed

    Jianwei He, Takashi Sakamoto, Youtao Song, Akira Saito, Akihito Harada, Hiroyuki Azakami, Akio Kato

    FEBS Letters   579 ( 11 )   2277 - 2283   2005.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.febslet.2005.03.019

▼display all

Presentations

  • High-throughput single cell epigenomic profiling using chromatin integration labelling-sequence International conference

    Akihito Harada, @Kazumitsu Maehara, @Yasuyuki Ohkawa

    Keystone Symposia on Molecular and Cellular Biology  2019.3 

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    Event date: 2019.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Canada  

  • Functional analysis of novel histone variant H3mm13 in skeletal muscle to Histone variants International conference

    Harada A

    EMBO Workshop Histone Variants  2017.6 

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    Event date: 2017.9

    Language:English  

    Country:Germany  

  • 細胞状態変化を計測するマルチオミクス技術開発 Invited

    原田 哲仁、富松 航佑

    第46回日本分子生物学会  2023.12 

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    Event date: 2023.12 - 2024.12

    Language:Japanese  

    Venue:神戸ポートアイランド   Country:Japan  

  • 単一細胞マルチエピゲノム解析による分化過程のクロマチンダイナミクスの可視化 Invited

    原田哲仁、藤井健、前原一満、木村宏、大川恭行

    第46回日本分子生物学会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • High-throughput single cell multi-epigenomic profiling using chromatin integration labelling-sequence International conference

    Akihito Harada

    Cell Symposia The Conceptual Power of Single Cell Biology  2023.8 

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    Event date: 2023.8 - 2024.8

    Language:Japanese  

    Country:United States  

  • 細胞状態変化を計測するためのマルチオミクス技術開発 Invited

    原田哲仁、富松航佑

    第16回日本エピジェネティクス研究会年会  2023.6 

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    Event date: 2023.6

    Language:Japanese  

    Venue:学術総合センター 一橋講堂   Country:Japan  

  • 単一細胞エピゲノム解析を用いたクロマチンマッピングによるゲノム機能の解明 Invited

    原⽥ 哲仁、藤井 健、前原 ⼀満、⽊村 宏、⼤川 恭⾏

    第45回日本分子生物学会  2022.6 

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 単一細胞エピゲノム解析によるゲノム動作原理の理解

    原田 哲仁

    第15回日本エピジェネティクス研究会  2022.6 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学医学部百年講堂   Country:Japan  

    a

  • Epigenomic lineage tracing toward the understanding acquisition of tissue-specific gene expression profile Invited

    Akihito Harada

    2021.9 

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    Event date: 2022.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 組織特異的な遺伝子発現機構の解明 Invited

    原田哲仁

    筑波大学TARAセミナー  2022.4 

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    Event date: 2022.4 - 2023.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  • ChIL法による単一細胞プロファイリング Invited

    第45回日本分子生物学会年会  2021.12 

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    Event date: 2021.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 単一細胞解析の最前線 Invited

    原田哲仁

    山口大学年次セミナー  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • ChIL法による単一細胞エピゲノムプロファイリング

    原田哲仁、前原一満、半田 哲也、木村 宏、大川 恭行

    第43回日本分子生物学会  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

    Analysis of chromatin states is regularly performed in development and disease research of multicellular organisms. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) has become the standard technology for genome-wide analysisof chromatin status in cells or tissues. However, ChIP-seq analysis in a small n
    umber of cells, including single cells, is still difficult to analyze due to low recover chromatin preparation. Recently, we have established a immunoprecipitation-free epigenomic profiling method based on immunostaining named chromatin integration labeling followed by sequencing (ChIL-seq). ChIL-seq can be started from a single cell on a well of a multi-well plate, and the level and spatial distr
    ibution of the target in the same cell can be analyzed by fluorescence microscop
    y. Furthermore, using combinatorial indexing methods, we demonstrate to detect h
    istone modifications such as H3K4me3 at a thousand of cells each single cell. In
    this talk, we will report on the progress of our research towards high-throughp
    ut of ChIL-seq at the single cell level.

  • ヒストンH3バリアントの選択的取り込みによる組織特異的な遺伝子発現制御

    原田 哲仁, 小松 哲郎, 前原 一満, 近藤 友佳理, 田中 かおり, 桑門 温子, 佐藤 優子, 木村 宏, 林 克彦, 小野 悠介, 竹本 龍也, 胡桃坂 仁志, 大川 恭行

    第47回日本分子生物学会年会  2019.12 

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    Event date: 2019.12

    Language:Japanese  

    Venue:福岡国際会議場   Country:Japan  

  • 単一細胞multi-omicsによりシンギュラリティ一細胞同定技術の開発

    原田哲仁

    新学術領域研究「シンギュラリティ生物学」第2回領域会議  2019.5 

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    Event date: 2019.12

    Language:Japanese  

    Venue:淡路夢舞台国際会議場   Country:Japan  

  • ChIL法を用いたエピゲノムおよび転写量を同時計測する技術開発

    原田哲仁、前原一満、半田哲也、木村宏、大川恭行

    新学術領域研究「クロマチン潜在能」第2回班会議  2019.6 

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    Event date: 2019.12

    Language:Japanese  

    Venue:ホテル竹島   Country:Japan  

  • Analysis of genomic structures responsible for tissue-specific gene expression

    2019.11 

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    Event date: 2019.12

    Language:Japanese  

    Country:Japan  

  • 極微量サンプルからのクロマチン修飾解析を可能にするChIL-seqの紹介 Invited

    原田哲仁

    トランスオミクス医学研究拠点ネットワーク形成事業 技術講習会  2019.3 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京医科歯科大学 M&Dタワー11階 大学院講義室3   Country:Japan  

  • 生殖細胞におけるヒストンバリアントによるゲノムマーキング機構の解明

    原田 哲仁

    新学術領域 生殖細胞のエピゲノムダイナミクスとその制御 取りまとめ公開シンポジウム  2018.12 

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    Event date: 2018.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京都教育文化センター   Country:Japan  

  • 非免疫沈降法ChILT法による少数細胞のエピゲノム解析

    原田哲仁、半田哲也、野上順平、有村泰宏、関根慧、中尾勝、藤田理沙、前原一満、胡桃坂仁志、木村宏、大川恭行

    2017年度生命科学系学会合同年次大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸ポートアイランド   Country:Japan  

  • 生殖細胞におけるヒストンバリアントによるゲノムマーキング機構の解明

    原田哲仁

    生殖細胞のエピゲノムダイナミクスとその制御 第5回公開シンポジウム  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:つくばノバホール   Country:Japan  

  • 急性期の筋萎縮過程における遺伝子発現変動解析

    原田 哲仁, 岩崎 健, 横田 和也, 岡田 誠司, 大川 恭行

    日本農芸化学会2017年度大会  2017.3 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都女子大(京都府)   Country:Japan  

  • Functional switching of histone H3 variants in myogenesis Invited

    2017.2 

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    Event date: 2017.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • エピジェネティック破綻により引き起こされた筋肉腫の解析 Invited

    原田 哲仁

    公益財団法人福岡県すこやか健康事業団平成28年度がん研究助成金優秀賞受賞講演  2017.1 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:西鉄グランドホテル(福岡県福岡市)   Country:Japan  

  • ヒストンH3バリアントの発現変動は筋萎縮過程の初期イベントである

    原田 哲仁, 岩崎 健, 横田 和也, 岡田 誠司, 大川 恭行

    第39回日本分子生物学会年会  2016.11 

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    Event date: 2016.11 - 2016.12

    Language:Japanese  

    Venue:パシフィコ横浜(神奈川県横浜市)   Country:Japan  

  • Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration. International conference

    Harada A

    2017.9 

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    Event date: 2016.9 - 2017.9

    Language:English  

    Country:Germany  

  • 骨格筋分化におけるクロマチン構造解析-トランスクリプトミクスで理解する骨格筋形成 Invited

    原田 哲仁

    日本農芸化学会中四国支部 支部創立15周年記念 第24回若手シンポジウム  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:水産大学校 (山口県下関市)   Country:Japan  

  • 生殖細胞の品質の定量に向けて 光を用いた空間トランスクリプトーム解析

    木村 龍一, 本田 瑞季, 原田 哲仁, 前原 一満, 田中 かおり, 大川 恭行, 沖 真弥

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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    Language:English  

  • 抗SARS-CoV-2免疫反応および抗体産生株 ヒストン変異体H3.3の発現が形質細胞の分化を制御する(Anti SARS-CoV-2 immune responses and Antibody producers Histone variant H3.3 expression controls the plasma cell differentiation)

    Saito Yuichi, Baba Yoshihiro, Ohkawa Yasuyuki, Harada Akihito

    日本免疫学会総会・学術集会記録  2022.11  (NPO)日本免疫学会

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    Language:English  

  • 形質細胞の分化はヒストン変異体H3.3の発現によって制御される(Plasma cell differentiation is regulated by the expression of histone variant H3.3)

    Saito Yuichi, Murakami Daisuke, Baba Yoshihiro, Harada Akihito, Ohkawa Yasuyuki, Takashi Nakagawa

    日本耳鼻咽喉科頭頸部外科学会会報  2024.4  (一社)日本耳鼻咽喉科頭頸部外科学会

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    Language:English  

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MISC

  • クロマチン構造における転写制御の理解に向けた技術開発

    原田哲仁, 富松航佑, 武 千湄, 大川恭行

    生体の科学   2023.6

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  • 1細胞エピゲノム

    原田 哲仁, 大川 恭行

    月刊「細胞」   2023.6

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  • 【1細胞解析技術】1細胞エピゲノム

    原田 哲仁, 大川 恭行

    細胞   55 ( 7 )   372 - 375   2023.6   ISSN:1346-7557

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    Language:Japanese   Publisher:(株)ニュー・サイエンス社  

    ヒトのほぼすべての種類の細胞は同一のゲノムを共有している。この同一のゲノムを持つ異なる種類の細胞の特性と機能は細胞のエピゲノムによって決定される。1細胞RNA-seqによって細胞集団の不均一性が明らかとなり,エピゲノムについても1細胞レベルの解析が求められるようになっている。1細胞エピゲノムは,ここ10年あまりで著しく進歩し,我々研究者はバルク法では見えなかった細胞間の不均一性や希少細胞集団の特性評価などの問題に取り組むことができるようになった。ここでは,個々の細胞レベルでの高解像度エピゲノム解析技術に向けた取り組みについて,代表的な技術を紹介する。(著者抄録)

  • 【クロマチンによる転写制御機構の最前線】クロマチンと転写制御に関する新技術 クロマチン構造における転写制御の理解に向けた技術開発

    原田 哲仁, 富松 航佑, 武 千び, 大川 恭行

    生体の科学   74 ( 3 )   255 - 259   2023.6   ISSN:0370-9531

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    Language:Japanese   Publisher:(公財)金原一郎記念医学医療振興財団  

    <文献概要>ヒトのほぼすべての種類の細胞は同一のゲノムを持つ。この同一のゲノムを持つ異なる種類の細胞の特性と機能は,遺伝子が存在するクロマチン構造の転写における選択性により支配されている。したがって,転写はRNAポリメラーゼIIによりゲノムDNAからRNAが合成されるプロセスという側面に加えて,異なるエピゲノム状態を判別し,適切な遺伝子発現を選択する過程と言える。近年の1細胞解析技術の高感度化,高深度化に伴い,細胞の型のみならず,細胞状態変化まで遺伝子発現の総体であるトランスクリプトームで区別可能になりつつある。一方で,これら細胞の状態変化が可塑的な範囲か,あるいは細胞型として不可逆的な変化を示したのかは判定が困難である。改めて,クロマチンレベルの遺伝子発現解析の重要性が高まっていると言えよう。本稿では,転写の場であるクロマチン構造変化と転写を測定する試みについて代表的な技術を紹介したい。

  • 少数細胞エピゲノム解析技術の開発

    原田 哲仁, 大川 恭行

    生化学 テクニカルノート   2021.6

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  • 1細胞エピゲノム解析技術開発の最前線 Reviewed

    大川 恭行,原田 哲仁,前原 一満

    医歯薬出版株式会社 週刊医学のあゆみ 2021年 Vol278No.10:912-917   2021.3

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  • 骨格筋研究のための最先端解析技術

    原田 哲仁,大川 恭行

    羊土社 実験医学 2020年10月号 Vol.38 No.16:2686-2692   2020.9

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  • シングルセルでのエピゲノム情報の計測技術

    原田 哲仁, 大川 恭行

    羊土社 実験医学 2020年 Vol.38   2020.4

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  • クロマチン挿入標識法(ChIL)による単一細胞エピゲノム解析

    原田 哲仁,大川 恭行

    羊土社 実験医学 2019年増刊号Vol.37   2019.12

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  • 発生起源の違いに着目した身体内骨格筋の不均一性と筋再性制御の違い

    吉岡 潔志, 瀬古 大暉, 北嶋 康雄, 土屋 吉史, 野上 順平, 原田 哲仁, 大川 恭行, 岡崎 成弘, 米倉 暁彦, 千葉 恒, 大場 誠悟, 住田 吉慶, 朝比奈 泉, 小野 悠介

    日本筋学会学術集会プログラム・抄録集   2018.8

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  • Temporal regulation of chromatin during myoblast differentiation.

    Harada A, Ohkawa Y, Imbalzano AN

    Semin Cell Dev Biol   2017.12

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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Industrial property rights

Patent   Number of applications: 2   Number of registrations: 0
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Professional Memberships

  • The Molecular Biology Society of Japan

  • The Japan Society for Bioscience, Biotechnology, and Agrochemistry

  • The Japanese Society for Epigenetics

  • The Japan Society for Bioscience, Biotechnology, and Agrochemistry

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  • The Molecular Biology Society of Japan

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Academic Activities

  • 組織委員

    第15回日本エピジェネティクス研究会年会  ( 九州大学医学部百年講堂 ) 2022.6

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Research Projects

  • 細胞競合における細胞間相互作用を計測するための空間オミクス技術開発

    2023.6 - 2025.6

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    Authorship:Principal investigator 

  • 精子形成における体細胞型H3から精子型H3T/tへのヒストン置換の意義の解明

    2023.4 - 2026.3

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  • 精子形成における体細胞型H3から精子型H3T/tへのヒストン置換の意義の解明

    Grant number:23H02394  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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  • 精子形成における体細胞型H3から精子型H3T/tへのヒストン置換の意義の解明

    Grant number:23K27087  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    原田 哲仁

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    Grant type:Scientific research funding

    精子形成過程では、「次世代へ伝播される遺伝情報」を形成するためクロマチン構造の再構成が起こる。この過程で起こる精子形成固有の現象が、体細胞型のヒストンから精巣特異的ヒストンへの置換である。研究代表者らは、最近、独自のエピゲノム解析からヒストンH3tは体細胞型 ヒストンH3.1/2と分化初期段階で入れ代わることを示唆する結果を得た。つまり、精子形成過程ではH3tが精子形成を進める特別なゲノム構造 を作り出している可能性が高い。そこで、本研究では、独自のエピゲノム解析技術と空間オミクス技術を駆使し、体細胞型ヒストンから精子特 異的ヒストンバリアントH3tへの置換の意義の解明に迫る。

    CiNii Research

  • 精子形成における体細胞型H3から精子型H3T/tへのヒストン置換の意義の解明

    Grant number:23H02394  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 無精子症における精巣内内分泌環境に着目した単一細胞トランスクリプトーム解析

    2022.4 - 2025.3

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    Authorship:Coinvestigator(s) 

  • 無精子症における精巣内内分泌環境に着目した単一細胞トランスクリプトーム解析

    Grant number:22K09475  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 無精子症における精巣内内分泌環境に着目した単一細胞トランスクリプトーム解析

    Grant number:22K09475  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 競合的コミュニケーションから迫る多細胞生命システムの自律性

    Grant number:21H05283  2021 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 競合的コミュニケーションから迫る多細胞生命システムの自律性

    Grant number:21H05283  2021 - 2025

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 細胞競合における細胞間相互作用を計測するための空間オミクス技術開発

    Grant number:21H05292  2021 - 2025

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(A)

    原田 哲仁

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    細胞競合は細胞間の相互作用を介した細胞排除現象であり、多細胞生命システムが自身の構造や機能を最適化する「自律性」を生み出す要の一つであると考えられる。細胞競合のプロセスを完全に理解するには、、細胞集団内の細胞間にわずかな性質の差を細胞間の時空間的な相互作用と遺伝子発現・シグナル変化を単一細胞レベルで定量的に解析する必要がある。そこで本研究では、これまでに開発した独自の空間オミクス解析技術を基盤として、細胞競合における細胞間相互作用を計測するための新たな空間マルチオミクス技術の開発と独自空間オミクス技術を駆使し、本領域内の細胞競合の分子基盤と多細胞生命システムの自律性生成原理の理解に迫る。

    CiNii Research

  • 革新的Perturb-ChIL法を用いた網羅的なクロマチン構造解析研究

    Grant number:21H00430  2021 - 2022

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 位置情報を保持したシンギュラリティ細胞の遺伝子発現測定技術の開発

    Grant number:21H00430  2021

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 単一細胞解析によるヘルペスウイルス慢性感染の分子基盤の解明

    2020 - 2022

    国立研究開発法人 日本医療研究開発機構

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 全能性獲得機構の解明のためのmulti-omics技術の開発

    Grant number:20H05368  2020 - 2021

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

    原田 哲仁

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    Grant type:Scientific research funding

    全能性の獲得には、様々なエピゲノム因子が関わると考えられるが、その全貌は明らかとなっていない。このような全能性因子の解析のためには、どの因子が協調して機能しているのかを分類するための同時性を担保した解析手法が必須である。そこで、本研究では同時性を担保するクロマチン構造解析と転写産物を同時に取得する技術multi-ChIL omicsの開発を目指す。本研究では、開発したmulti-ChIL omics技術の少数細胞での適用とハイスループット化を目標とし開発を進める。

    CiNii Research

  • 組織特異的ゲノム構造の再構築技術の開発

    2019 - 2022

    戦略的創造研究推進事業 (文部科学省)

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    Authorship:Principal investigator  Grant type:Contract research

  • 精子特異的ヒストンバリアントH3T/tを起点としたクロマチンダイナミクスの解明

    Grant number:19H03211  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    原田 哲仁

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    Grant type:Scientific research funding

    精子形成過程では、「次世代へ伝播される遺伝情報」を形成するためクロマチン構造の再構成が起こる。この過程で起こる精子形成固有の現象が、体細胞型のヒストンから精巣特異的ヒストンへの置換である。我々はこれまでに、精子細胞には複数の機能未知のヒストンが発現しており、そのうちヒストンH3tは精子形成の最初期に必須であることを報告し、精子形成過程ではH3tを起点に従来の知見を超えた多彩且つ複雑なヒストン置換が起こっている可能性が高いことを示した。そこで、本研究では、体細胞型ヒストンから精子特異的ヒストンバリアントH3tへの置換を起点として、精子形成過程でのクロマチン構造変換とその制御機構の解明を目指す。

    CiNii Research

  • 単一細胞遺伝子解析によるヒト造精機能障害の分子機構の解明

    Grant number:19K09712  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    白石 晃司, 原田 哲仁, 大川 恭行

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    Grant type:Scientific research funding

    非閉塞性無精子症に対する根本的な治療法は存在しない。In vitroヒト精細管培養による精子形成の確立に向け、ヒト造精機能障害、特にearly maturation arrest症例について顕微鏡下精巣内精子採取術時に採取された精細胞の単一細胞網羅的遺伝子解析を行う。
    1)分化・増殖が停止する細胞と正常な細胞の遺伝子発現の比較を次世代シーケンサーを用いて単一細胞レベルで行い、造精機能障害の分子生物学的メカニズムを明らかにする。
    2)Leydig細胞を含んだヒト精細管のin vitro培養を行い、培養条件の工夫により精細胞の分化・増殖を生じうるか、また培養後の精細胞の遺伝子発現を解析する。

    CiNii Research

  • 単一細胞multi-omicsによるシンギュラリティー細胞同定技術の開発

    Grant number:19H05425  2019 - 2020

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

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    Grant type:Scientific research funding

  • 単一細胞超高解像度解析を可能にするゲノム3次元構造解析技術の開発

    Grant number:18K19432  2018 - 2019

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

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    Grant type:Scientific research funding

  • エピジェネティック破綻により引き起こされた筋肉腫の解析

    2016 - 2018

    公益財団法人福岡県すこやか健康事業団平成28年度がん研究助成金

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    Authorship:Principal investigator  Grant type:Contract research

  • 生殖細胞におけるヒストンバリアントによるゲノムマーキング機構の解明

    Grant number:16H01219  2016 - 2017

    日本学術振興会・文部科学省  科学研究費助成事業  新学術領域研究

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    Grant type:Scientific research funding

  • 骨格筋分化における新規ヒストンH3バリアントH3mm7の遺伝子選択機構の解明

    Grant number:15K18457  2015 - 2016

    科学研究費助成事業  若手研究(B)

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    Grant type:Scientific research funding

  • 細胞分化運命決定の遺伝子選択メカニズムの解明

    Grant number:13J04024  2013 - 2015

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

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Class subject

  • 生命医科学特論Ⅲ

    2024.6 - 2024.8   Summer quarter

  • Topics in medical life sciences Ⅲ

    2024.6 - 2024.8   Summer quarter

  • Topics in medical life sciences Ⅲ

    2023.6 - 2023.8   Summer quarter

  • 生命医科学特論Ⅲ

    2023.6 - 2023.8   Summer quarter

  • 生命医科学特論Ⅰ

    2022.6 - 2022.8   Summer quarter

  • 生命医科学特論Ⅰ

    2021.6 - 2021.8   Summer quarter

  • Topics in medical life sciences Ⅰ

    2021.6 - 2021.8   Summer quarter

  • 生命医科学特論I

    2020.6 - 2020.8   Summer quarter

  • 最新トピックスから学ぶ生命科学入門

    2019.10 - 2019.12   Fall quarter

  • 生命医科学特論I

    2019.4 - 2019.6   Spring quarter

  • 生命医科学特論I

    2018.4 - 2018.6   Spring quarter

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FD Participation

  • 2024.9   Role:Participation   Title:馬出地区4部局合同男女共同参画FD/ 病院きらめきプロジェクト講演会

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2024.3   Role:Participation   Title:有体物管理センターの業務および成果有体物収入の配分率の変更について

    Organizer:University-wide

  • 2023.8   Role:Participation   Title:令和5年度4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.12   Role:Participation   Title:医学系学府教育FD「医学系大学院プログラムの進化と深化をめざして」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2022.3   Role:Participation   Title:新M2Bシステムの使い方 ~新機能を中心に紹介します~(3/17)

    Organizer:University-wide

  • 2021.12   Role:Participation   Title:医学系学府教育FD「学術論文の購読と投稿とこれから」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.7   Role:Participation   Title:生体防御医学研究所FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2021.2   Role:Participation   Title:馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.11   Role:Participation   Title:馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.9   Role:Participation   Title:M2B学習支援システム講習会(オンライン開催)◇初級編・中級編◇10:00~12:00

    Organizer:University-wide

  • 2020.8   Role:Participation   Title:【IDE大学セミナー】大学教職員の多様な働き方について

  • 2019.10   Role:Participation   Title:馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2018.10   Role:Participation   Title:馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

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