Updated on 2025/06/09

Information

 

写真a

 
FUJIOKA YUTAKA
 
Organization
Faculty of Medical Sciences Department of Clinical Medicine Assistant Professor
School of Medicine Department of Medicine（Concurrent）
Title
Assistant Professor
Profile
Treatment of diseases in the field of neurosurgery. Education for residents. Clinical studies mainly on malignant brain tumors.
External link

Research Interests・Research Keywords

  • Research theme： liquid biopsy for malignant brain tumor

    Keyword： liquid biopsy

    Research period： 2018.4 - 2021.5

Papers

  • Molecular diagnosis of diffuse glioma using a chip-based digital PCR system to analyze IDH, TERT, and H3 mutations in the cerebrospinal fluid Invited Reviewed International journal

    Yutaka Fujioka, Nobuhiro Hata, Yojiro Akagi, Daisuke Kuga, Ryusuke Hatae, Yuhei Sangatsuda, Yuhei Michiwaki, Takeo Amemiya, Kosuke Takigawa, Yusuke Funakoshi, Aki Sako, Toru Iwaki, Koji Iihara & Masahiro Mizoguchi

    Journal of Neuro-Onchology   2021.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Purpose
    Conventional genetic analyzers require surgically obtained tumor tissues to confirm the molecular diagnosis of diffuse glioma. Recent technical breakthroughs have enabled increased utilization of cell-free tumor DNA (ctDNA) in body fluids as a reliable resource for molecular diagnosis in various cancers. Here, we tested the application of a chip-based digital PCR system for the less invasive diagnosis (i.e., liquid biopsy) of diffuse glioma using the cerebrospinal fluid (CSF).

    Methods
    CSF samples from 34 patients with diffuse glioma were collected from the surgical field during craniotomy. Preoperative lumbar CSF collection was also performed in 11 patients. Extracted ctDNA was used to analyze diagnostic point mutations in IDH1 R132H, TERT promoter (C228T and C250T), and H3F3A (K27M) on the QuantStudio® 3D Digital PCR System. These results were compared with their corresponding tumor DNA samples.

    Results
    We detected either of the diagnostic mutations in tumor DNA samples from 28 of 34 patients. Among them, we achieved precise molecular diagnoses using intracranial CSF in 20 (71%). Univariate analyses revealed that the World Health Organization (WHO) grade (p = 0.0034), radiographic enhancement (p = 0.0006), and Mib1 index (p = 0.01) were significant predictors of precise CSF-based molecular diagnosis. We precisely diagnosed WHO grade III or IV diffuse gliomas using lumbar CSF obtained from 6 (87%) of 7 patients with tumors harboring any mutation.

    Conclusion
    We established a novel, non-invasive molecular diagnostic method using a chip-based digital PCR system targeting ctDNA derived from CSF with high sensitivity and specificity, especially for high-grade gliomas.

  • Liquid biopsy with multiplex ligation-dependent probe amplification targeting cell-free tumor DNA in cerebrospinal fluid from patients with adult diffuse glioma Reviewed International journal

    Ryosuke Otsuji, Yutaka Fujioka, Nobuhiro Hata, Daisuke Kuga, Yuhei Sangatsuda, Kosuke Takigawa, Yusuke Funakoshi, Aki Sako, Hidetaka Yamamoto, Akira Nakamizo, Masahiro Mizoguchi, Koji Yoshimoto

    Neuro-Oncology Advances   2022.11

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.1093/noajnl/vdac178

  • Clinical significance of CDKN2A homozygous deletion in combination with methylated MGMT status for IDH‐wildtype glioblastoma. Reviewed International journal

    Yusuke Funakoshi, Nobuhiro Hata,corresponding author Kosuke Takigawa, Hideyuki Arita, Daisuke Kuga, Ryusuke Hatae, Yuhei Sangatsuda, Yutaka Fujioka, Aki Sako, Toru Umehara, Tadamasa Yoshitake, Osamu Togao, Akio Hiwatashi, Koji Yoshimoto, Toru Iwaki, and Masahiro Mizoguchi

    Cancer Medicine   2022.6

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • CD206 Expression in Induced Microglia-Like Cells From Peripheral Blood as a Surrogate Biomarker for the Specific Immune Microenvironment of Neurosurgical Diseases Including Glioma Reviewed International journal

    Shunya Tanaka, Masahiro Ohgidani, Nobuhiro Hata, Shogo Inamine, Noriaki Sagata, Noritoshi Shirouzu, Nobutaka Mukae, Satoshi O. Suzuki, Hideomi Hamasaki, Ryusuke Hatae, Yuhei Sangatsuda, Yutaka Fujioka, Kosuke Takigawa, Yusuke Funakoshi, Toru Iwaki, Masako Hosoi, Koji Iihara, Masahiro Mizoguchi and Takahiro A. Kato

    Frontiers in immunology,   2021.7

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Clinical implications of molecular analysis in diffuse glioma stratification Reviewed International journal

    Masahiro Mizoguchi, Nobuhiro Hata, Daisuke Kuga, Ryusuke Hatae, Yojiro Akagi, Yuhei Sangatsuda, Yutaka Fujioka, Kosuke Takigawa, Yusuke Funakoshi, Satoshi O. Suzuki & Toru Iwaki

    Brain Tumor Pathology   2021.7

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Volumetric study reveals the relationship between outcome and early radiographic response during bevacizumab-containing chemoradiotherapy for unresectable glioblastoma. Reviewed International journal

    Kosuke Takigawa, Nobuhiro Hata, Yuhei Michiwaki, Akio Hiwatashi, Hajime Yonezawa, Daisuke Kuga, Ryusuke Hatae, Yuhei Sangatsuda, Yutaka Fujioka, Yusuke Funakoshi, Ryosuke Otsuji, Aki Sako, Osamu Togao, Takashi Yoshiura, Koji Yoshimoto & Masahiro Mizoguchi

    Journal of Neuro-oncology   2021.6

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

▼display all

Presentations

  • 初発脳腫瘍における髄液中cell free DNA量の検討

    藤岡 寛, 尾辻 亮介, 舟越 勇介, 瀧川 浩介, 三月田 祐平, 空閑 太亮, 秦 暢宏, 溝口 昌弘 吉本 幸司 初発脳腫瘍における髄液中cell free DNA量の検討

    第39回日本脳腫瘍学会学術総会.  2021.11 

    　More details

    Event date： 2022.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：兵庫県   Country：Japan  

  • 髄液中cell free tumor DNAを用いたDiffuse gliomaの分子診断

    藤岡寛、秦　暢宏、空閑太亮、赤木洋二郎、波多江龍亮、三月田祐平、瀧川浩介、舟越勇介、溝口昌弘

    一般社団法人日本脳神経外科学会第78回学術総会  2020.10 

    　More details

    Event date： 2020.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：web   Country：Japan  

  • 当院におけるRosette-forming glioneuronal tumorの経験

    藤岡　寛、瀧川浩介、三月田祐平、空閑太亮、秦　暢宏、溝口昌宏、吉本幸司

    第27回日本脳腫瘍の外科学会  2022.10 

    　More details

    Event date： 2022.10 - 2023.6

    Language：Japanese  

    Country：Japan  

  • Diffuse gliomaに対する髄液中cell free DNAを用いたliquid biopsyの現状と課題

    藤岡 寛, 尾辻 亮介, 舟越 勇介, 瀧川 浩介, 三月田 祐平, 空閑 太亮, 秦 暢宏, 中溝 玲,溝口 昌弘, 吉本 幸司

    第81回脳神経外科学会総会  2022.9 

    　More details

    Event date： 2022.9 - 2023.6

    Language：Japanese  

    Country：Japan  

  • Diffuse midline gliomaに対するcell free DNAを用いたliquid biopsy

    藤岡 寛、尾辻 亮介、舟越 勇介、瀧川 浩介、三月田 祐平、空閑 太亮秦　暢宏、溝口 昌弘、吉本 幸司

    一般社団法人日本脳神経外科学会第80回学術総会  2021.10 

    　More details

    Event date： 2022.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：神奈川   Country：Japan  

Professional Memberships

  • The Japan Neurosurgical Society

  • The Japan Stroke Society

Research Projects

  • cfDNAを用いる神経膠腫liquid biopsyの発展

    Grant number：22K16690  2022 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Early-Career Scientists

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

  • 神経膠腫に対するliquid biopsy

    2021

    公益財団法人ふくおか公衆衛生推進機構　がん研究助成　奨励賞

    　 More details

    Authorship：Principal investigator  Grant type：Contract research