Updated on 2024/11/12

Information

 

写真a

 
MITARAI HIROMI
 
Organization
Kyushu University Hospital General Dentistry Assistant Professor
Abolition organization Comprehensive Dentistry(Concurrent)
School of Dentistry Department of Dentistry(Concurrent)
Graduate School of Dental Science (Concurrent)
Graduate School of Dental Science Department of Dental Science(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Profile
研究:根尖性歯周炎、歯根破折や歯周病によって失われた歯根膜組織再生を目指して研究を行っている。特に、ヒト歯根膜細胞に幹細胞特性を付与する因子の検討・解析を行っている。また、細菌感染によって惹起された歯髄炎に対し、歯髄炎抑制効果ならびに抗菌作用のある因子を探索・解析を行っている。 教育:歯科医師の卒後研修指導、大学院生ならびに学部学生の研究指導を担当している。 臨床:口腔総合診療を行っている。特に歯内治療、修復治療を専門に行っている。
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Degree

  • Doctor of Dental Science

Research History

  • Kyushu University Assistant Professor

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    Country:Japan

Research Interests・Research Keywords

  • Research theme:歯根膜組織

    Keyword:歯根膜組織

    Research period: 2024

  • Research theme:Development of new periodontal ligament regeneration therapy

    Keyword:periodontal ligament, stem cell, exosome

    Research period: 2018.6

  • Research theme:The effect of Transgelin on homeostasis of human periodontal ligament cells

    Keyword:human periodontal ligament cells

    Research period: 2013.4 - 2017.3

Papers

  • Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Reviewed International journal

    2023.4

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    Background/purpose: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells.

    Materials and methods: RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells.

    Results: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2-23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2-23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1-stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3.

    Conclusion: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies.

  • 患者情報やコミュニケーション技法を積極的に活用し歯科治療が可能となった 1 症例 Reviewed

    信太実有, 御手洗裕美, 和田尚久

    2022.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells. Reviewed International journal

    52 ( 6 )   984 - 993   2017.12

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    BACKGROUND AND OBJECTIVE:
    Human periodontal ligament cells (HPDLCs) express transforming growth factor-β1 (TGF-β1) that regulates differentiation and proliferation, and plays key roles in homeostasis of PDL tissue. Transgelin is a cytoskeleton-associated protein with an Smad-binding element in its gene promoter region. In this study, we examined the localization and potential function of transgelin in PDL tissue and cells.

    MATERIAL AND METHODS:
    Microarray analysis of HPDLC lines (2-14, 2-23 and 2-52) was performed. Expression of transgelin in HPDLCs was examined by quantitative reverse transcription-polymerase chain reaction, immunofluorescence staining and western blot analysis. Effects of TGF-β1 and its signaling inhibitor, SB431542, on transgelin expression in HPDLCs were examined by western blot analysis. The effects of transgelin knockdown by small interfering RNA (siRNA) on HPDLC proliferation stimulated by TGF-β1 were assessed by WST-1 assay.

    RESULTS:
    In microarray and quantitative reverse transcription-polymerase chain reaction analyses, the expression levels of transgelin (TAGLN) in 2-14 and 2-23 cells, which highly expressed PDL markers such as periostin (POSTN), tissue non-specific alkaline phosphatase (ALPL), α-smooth muscle actin (ACTA2) and type I collagen A1 (COL1A1), was significantly higher than those in 2-52 cells that expressed PDL markers weakly. Immunohistochemical and immunofluorescence staining revealed expression of transgelin in rat PDL tissue and HPDLCs. In HPDLCs, TGF-β1 treatment upregulated transgelin expression, whereas inhibition of the type 1 TGF-β1 receptor by SB431542 suppressed this upregulation. Furthermore, TAGLN siRNA transfection did not promote the proliferation of HPDLCs treated with TGF-β1. The expression levels of CCNA2 and CCNE1, which regulate DNA synthesis and mitosis through the cell cycle, were also not upregulated in HPDLCs transfected with TAGLN siRNA.

    CONCLUSION:
    Transgelin is expressed in PDL tissue and might have a role in HPDLC proliferation induced by TGF-β1 stimulation.

    DOI: 10.1111/jre.12466

  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria Reviewed International journal

    Harunobu Kamiya, Akira Haraguchi, Hiromi Mitarai, Asuka Yuda, Hiroko Wada, Wang Shuxin, Ran Ziqing, Sun Weihao, Naohisa Wada

    Journal of Infection and Chemotherapy   2024.4

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  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria. International journal

    Harunobu Kamiya, Akira Haraguchi, Hiromi Mitarai, Asuka Yuda, Hiroko Wada, Wang Shuxin, Ran Ziqing, Sun Weihao, Naohisa Wada

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   30 ( 4 )   306 - 314   2024.4   ISSN:1341-321X eISSN:1437-7780

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    Manuka oil and tea tree oil are essential oils with known antibacterial properties that are believed to be caused by one main component: terpinen-4-ol. Terpinen-4-ol has potent antibacterial activity against caries-related microorganisms. However, few studies have investigated the antimicrobial effects of terpinen-4-ol on bacteria in apical periodontitis. Thus, the objective of the present study was to evaluate the antibacterial and antibiofilm potential of terpinen-4-ol against Enterococcus faecalis, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum, which have all been detected in apical periodontitis. The minimum inhibitory and minimum bactericidal concentrations of terpinen-4-ol were determined to assess its activity against biofilms. The minimum inhibitory concentration of terpinen-4-ol was 0.25% against E. faecalis and F. nucleatum, 0.05% against P. gingivalis, and 0.1% against P. intermedia. The minimum bactericidal concentration of terpinen-4-ol was 1.0% against E. faecalis, 0.2% against P. gingivalis and P. intermedia, and 0.5% against F. nucleatum. In the biofilm evaluations, all terpinen-4-ol-treated bacteria had significant reductions in biofilm viability compared with controls in experiments assessing attachment inhibitory activity. Furthermore, structural alterations and decreased bacterial cell clumping were observed under scanning electron microscopy, and significantly decreased cell survival was noted using fluorescence microscopy. Together, these results suggest that terpinen-4-ol is a potential antibacterial agent with bactericidal properties, and can also act on established biofilms.

    DOI: 10.1016/j.jiac.2023.10.021

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  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria(タイトル和訳中)

    Kamiya Harunobu, Haraguchi Akira, Mitarai Hiromi, Yuda Asuka, Wada Hiroko, Wang Shuxin, Ran Ziqing, Sun Weihao, Wada Naohisa

    Journal of Infection and Chemotherapy   30 ( 4 )   306 - 314   2024.4   ISSN:1341-321X

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    Language:English   Publisher:エルゼビア・ジャパン(株)  

  • Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway. International journal

    Naati Fakatava, Hiromi Mitarai, Asuka Yuda, Akira Haraguchi, Hiroko Wada, Daigaku Hasegawa, Hidefumi Maeda, Naohisa Wada

    Journal of dental sciences   18 ( 2 )   567 - 576   2023.4   ISSN:1991-7902 eISSN:2213-8862

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND/PURPOSE: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells. MATERIALS AND METHODS: RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells. RESULTS: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2-23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2-23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1-stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3. CONCLUSION: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies.

    DOI: 10.1016/j.jds.2022.08.030

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  • 患者情報やコミュニケーション技法を積極的に活用し歯科治療が可能となった1症例

    信太 実有, 御手洗 裕美, 和田 尚久

    日本総合歯科学会雑誌   14   39 - 46   2022.10

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    Language:Japanese   Publisher:日本総合歯科学会  

    定期的な口腔内清掃を希望する患者が来院するが,歯周疾患の治癒・病状安定を維持している患者が多い一方,病状が進行し予後不良の歯が出現しているものの,様々な理由で治療や抜歯を希望しない患者もいる。今回,患者情報を参考に,様々なコミュニケーション技法を応用して患者と治療方針を検討した。その結果,抜歯を含めた全顎的な治療に取り組むことができた症例を経験したため報告する。症例は76歳,男性。「右上の歯が痛い。」という主訴で来院した。患者は九州大学病院口腔総合診療科で全顎的治療を行った後にSupportive Periodontal Therapy(SPT)へ移行したが,その後約5年にわたり「簡単に掃除をやってほしい。」との訴えが続いた。そのため,定期的な口内法X線写真撮影や歯周基本検査を含む,十分なSPTを行えていなかった。そこで,過去の患者情報から患者の考え方を考察し,(1)目標設定理論を応用し,何か処置をする前には,処置内容,所要時間,その処置が必要な理由をその都度伝えること,(2)"But You Are Free"(BYAF) compliance-gaining techniqueを応用した質問形式を取ること,(3)"Self Persuasion"自主説得理論を応用した治療の提案を行うことのコミュニケーション技法を応用して治療を開始した。その結果,患者は現症や治療方法を十分理解・納得し,予後不良と判断した歯の抜歯と,上顎即時義歯を製作することができた。以上のことから,患者情報や様々なコミュニケーション技法を応用することで,治療へ移行ができる可能性があると考えられた。(著者抄録)

  • Effects of ultraviolet irradiation equipment on endodontic disease–related bacteria

    Haraguchi A., Yoshida S., Takeshita M., Sumi Y., Mitarai H., Yuda A., Wada H., Nishimura F., Maeda H., Wada N.

    Lasers in Dental Science   6 ( 1 )   31 - 40   2022.3

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    Publisher:Lasers in Dental Science  

    Introduction: Dental caries and apical periodontitis are ones of the most prevalent chronic diseases and involve infection by cariogenic and endodontic bacteria. It can be said that the most required to cure is disinfection. We hypothesized that NB-UVB could be used for intraoral disinfection without affecting cells, and that it could be used in combination with TiO2 to disinfect complex root canals. The objectives were to investigate the effects of UV on dental pulp cells and oral bacteria and to evaluate the enhancement effect of a photocatalyst on bactericidal effects of UV irradiation. Methods: UV irradiation devices of UVB (310, 285 nm) and UVC (265 nm) were prepared. Cell proliferation and cytotoxicity assays after UV irradiation were performed using human dental pulp cells. The antibacterial activity of UV irradiation was investigated in Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, Actinomyces naeslundii, Porphyromonas gingivalis, and Fusobacterium nucleatum. A curved simulated root canal with plastic training block was used to evaluate the effect of combined UV and TiO2 treatment. Data were analyzed by one-way ANOVA (p < 0.05) followed by Tukey’s post hoc tests (p < 0.05). Results: Human dental pulp cell proliferation was decreased by 265 nm, 285 nm, and 310 nm UV irradiation, although 310 nm UV irradiation did not show cytotoxic effects on these cells. Oral bacterial growth was suppressed following UV irradiation at 285 nm and 265 nm. Viability of all bacteria significantly decreased with UV irradiation. In the curved simulated root canal, viability of E. faecalis in UV irradiation at 285 nm with long taper fibers was significantly decreased in the 300 s irradiation group. E. faecalis proliferation was inhibited by combined UV irradiation and TiO2 application with long taper fibers in the curved simulated root canal. Conclusions: The wavelength of UVB and UVC showed bactericidal effects on oral bacteria including caries-related bacteria and apical periodontitis–related bacteria while NB-UVB did not. UVB with longer fibers was more effective in disinfection on E. faecalis in curved simulated root canal, and the combined use of photocatalyst further improved the disinfection effect.

    DOI: 10.1007/s41547-021-00145-8

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  • MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells Reviewed International journal

    @Daigaku Hasegawa,@Kana Hasegawa,#Hiroshi Kaneko,@Shinichiro Yoshida,@Hiromi Mitarai,@Mai Arima,@Atsushi Tomokiyo,@Sayuri Hamano, @Hideki Sugii,@Naohisa Wada,@Tamotsu Kiyoshima,@Hidefumi Maeda

    Hindawi   2020.7

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  • Functions of beta2-adrenergic receptor in human periodontal ligament cells Invited Reviewed International journal

    Sayuri Hamano Atsushi Tomokiyo Daigaku Hasegawa Asuka Yuda Hideki Sugii Shinichiro Yoshida Hiromi Mitarai Naohisa Wada Hidefumi Maeda

    Journal of Cellular Biochemistry   2020.7

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  • R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/beta-catenin signaling pathway. Reviewed International journal

    Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H

    J Periodont Res.   54 ( 2 )   143 - 153   2019.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jre.12611

  • 骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する

    吉田晋一郎、和田尚久、長谷川大学、御手洗裕美、有馬麻衣、友清 淳、濱野さゆり、杉井英樹

    日歯保存誌   61 ( 6 )   343 - 353   2018.12

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  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling. Reviewed International journal

    Hasegawa D, Wada N, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Maeda H.

    J Cell Physiol.   2018.2

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  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling Reviewed

    Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda

    Journal of Cellular Physiology   233 ( 2 )   1752 - 1762   2018.2

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    DOI: 10.1002/jcp.26086

  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells Reviewed

    Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Shoko Fujino, Naohisa Wada, Hidefumi Maeda

    Stem Cells and Development   27 ( 2 )   100 - 111   2018.1

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    DOI: 10.1089/scd.2017.0077

  • Senescence and odontoblastic differentiation of dental pulp cells. Reviewed International journal

    Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H.

    J Cell Physiol.   234 ( 1 )   849 - 859   2018.1

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    DOI: 10.1002/jcp.26905

  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells. Reviewed International journal

    Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H.

    Stem Cells Dev.   2018.1

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  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells. Reviewed International journal

    Mizumachi H, Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Yuda A, Sugii H, Serita S, Mitarai H, Koori K, Wada N, Maeda H.

    Bone   2017.8

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  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells Reviewed

    Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda

    Bone   101   191 - 201   2017.8

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    DOI: 10.1016/j.bone.2017.05.012

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells Reviewed

    78   135 - 143   2017.6

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    Objective The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). Design A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. Results Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. Conclusions The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.

    DOI: 10.1016/j.archoralbio.2017.02.018

  • GDNF From Human Periodontal Ligament Cells Treated With Pro-Inflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells Reviewed

    Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Satoshi Monnouchi, Asuka Yuda, Hidefumi Maeda

    Journal of Cellular Biochemistry   118 ( 4 )   699 - 708   2017.4

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    DOI: 10.1002/jcb.25662

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells. Reviewed International journal

    2017.2

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    OBJECTIVE:

    The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs).
    DESIGN:

    A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR.
    RESULTS:

    Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs.
    CONCLUSIONS:

    The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.

  • Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells Reviewed

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, H. Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

    Journal of Dental Research   95 ( 11 )   1282 - 1290   2016.10

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    DOI: 10.1177/0022034516653085

  • Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. Invited Reviewed International journal

    Yoshida S, Wada N, Hasegawa D, Miyaji H, Mitarai H, Tomokiyo A, Hamano S, Maeda H.

    J Dent Res   2016.10

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  • Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression. Reviewed International journal

    2015.11

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    Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and Western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression.

  • Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression Reviewed

    230 ( 11 )   2647 - 2660   2015.11

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    Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and Western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression.

    DOI: 10.1002/jcp.24950

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Presentations

  • スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

    御手洗裕美、Naati Fakatava、王恕心、冉子晴、祐田明香、原口晃、孫偉浩、和田尚久

    日本歯科保存学会2023年度春季大会 (第158回)  2023.6 

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    Event date: 2023.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:くにびきメッセ   Country:Japan  

  • 術前の口腔内診査と診断の重要性を学んだ2つの症例 Invited

    御手洗 裕美

    第15回日本総合歯科学会学術大会  2022.11 

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    Event date: 2022.11 - 2023.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 術前の口腔内診査と診断の重要性を学んだ2つの症例 Invited

    御手洗 裕美

    第 15 回日本総合歯科学会学術大会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  • The Role of Acta2 in Periodontal Ligament cell stimulated with TGF-β1

    Fakatava Naati、御手洗裕美、祐田明香、長谷川大学、前田英史、和田尚久

    日本歯科保存学会  2020.6 

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    Event date: 2020.6

    Language:English  

    Venue:Web開催   Country:Japan  

  • Transgelinは、Integrinを介した細胞外基質への接着に関与する

    御手洗裕美、祐田明香、Fakatava Naati、長谷川大学、前田英史、和田尚久

    日本歯科保存学会  2020.6 

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    Event date: 2020.6

    Language:Japanese  

    Venue:Web開催   Country:Japan  

  • IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

    王恕心、御手洗裕美、冉子晴、祐田明香、孫偉浩、原口晃、前田英史、和田尚久

    日本歯科保存学会2023年度秋季大会 (第159回)  2023.11 

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    Event date: 2023.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:アクトシティ浜松   Country:Japan  

  • 著しい骨隆起を有する患者に対し包括的治療を行った症例

    渡邉護煕,御手洗裕美,王丸寛美,和田尚久

    第 16 回日本総合歯科学会学術大会  2023.10 

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    Event date: 2023.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本大学歯学部創設百周年記念講堂   Country:Japan  

  • ACTA2 regulates human PDL function via interaction with or without TGF-β1

    Naati Fakatava, 御手洗 裕美, 祐田 明香, 原口 晃、長谷川 大学、前田 英史、和田 尚久

    日本歯科保存学会2022年度春季学術大会(第156回)  2022.6 

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    Event date: 2022.6

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

    [OBJECTIVE] ACTA2 (alpha-smooth muscle actin; α-SMA), one of the cytoskeleton protein, is known to be expressed in periodontal ligament (PDL) tissue. It might be involved in PDL function such as proliferation and migration. Also ACTA2 is upregulated with transforming growth factor-β1 (TGF-β1) which exists in PDL tissue predominantly (Fujii et al, Cell Tissue Res. 2010). So it is assumed that ACTA2 is involved in TGF-β1-dependent function in human PDL cells, but little is known about them. Therefore, we focused on ACTA2 and examined its role within the PDL function via interaction with or without TGF-β1 to find its role in the remodeling of the PDL.
    [MATERIALS AND METHODS] Human PDL cell line 2-23, which was isolated from heterogeneous immortalized Human PDL cells (Hasegawa et al. J Cell Physiol. 2018) was used. (1) Western blotting analysis was performed to examine the protein expression of ACTA2. (2) ACTA2 or scramble siRNA were transfected to 2-23 for 48 h, and performed the WST-1 assay and scratch wound healing assay to analyze the proliferation and migration. (3) 2-23 cells were cultured with or without human recombinant TGF-β1 (rhTGF-β1: 10 ng/ml) for 24 h in the presence of ACTA2 or scramble siRNA. qRT-PCR was performed to examined the mRNA expression of PDL related genes; collagen1A1 (COL1A1), periostin (POSTN), and fibrillin1 (FBN-1). Picro-sirius red staining and sircol collagen assay were performed to analyze the collagen production. Western blotting analysis was performed to examine the phosphorylation of TGF-β1-related molecules; Smad2, Smad3, and YAP. All procedures were performed in compliance with requirements of the Institutional Review Board for Human Genome / Gene Research (approval number: 30-167) and Research Ethics Committee (approval number: 27-76) at Kyushu University.
    [RESULTS] ACTA2 protein expression was observed through Western blotting analysis in 2-23. After transfection with ACTA2 siRNA, cell proliferation and migration levels were significantly downregulated compared with scramble siRNA. The mRNA expression of ACTA2, COL1A1, POSTN, and FBN-1 was upregulated in 2-23 stimulated with TGF-β1. Those mRNA expression was significantly downregulated in the presence of ACTA2 siRNA stimulated with rhTGF-β1 compared with scramble siRNA. The amounts of collagen production were upregulated in 2-23 stimulated with rhTGF-β1 which were analyzed by picro-sirius red staining and sircol collagen assay. But after ACTA2 knockdown, the collagen production stimulated with rhTGF-β1 were significantly downregulated compared with scramble siRNA. We revealed that phosphorylation of Smad2 and Smad3 in 2-23 were observed at 15-min time point with TGF-β1, and phosphorylation of YAP was observed at 30-min time point with TGF-β1 by Western blotting analysis. However, after ACTA2 knockdown, at each time point, the phosphorylation of Smad2, Smad3, and YAP was downregulated after TGF-β1 stimulation.
    [DISCUSSION] In this research, ACTA2 was involved in proliferation and migration of human PDL cells. These results suggest that as cytoskeleton protein, ACAT2 itself is crucial for PDL function. In the presence of ACTA2 siRNA, upregulation of PDL related genes, collagen production, and phosphorylation of TGF-β1-related molecules were significantly downregulated, suggesting that ACTA2 might be a key factor for TGF-β1 function.
    [CONCLUSION] ACTA2 regulates human PDL function via interaction with or without TGF-β1.

  • 患者とのラポール形成で、抜歯即時義歯を製作できた1例

    信太実有、御手洗裕美、和田尚久

    第14回日本総合歯科学会総会・学術大会  2021.10 

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    Event date: 2021.10 - 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:Web開催   Country:Japan  

  • Terpinen-4-ol の根管内細菌に対する抗菌性の検討

    神谷治伸、原口晃、御手洗裕美、Fakatava Naati、祐田明香、前田英史、和田尚久

    日本歯科保存学会2022年度春季学術大会(第155回)  2021.10 

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    Event date: 2021.10 - 2021.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:Web開催   Country:Japan  

  • Effects of TNF-alpha on Senescent human dental pulp cells. Invited

    Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda.

    Kyudai Oral Bioscience & OBT Research Center Joint International Symposium  2019.3 

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    Event date: 2019.3

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • LGR4が未分化なヒト歯根膜細胞の増殖能,走化性および骨芽細胞様分化に及ぼす影響

    有馬麻衣,長谷川大学,吉田晋一郎,御手洗裕美,友清 淳,濱野さゆり,杉井英樹,和田尚久,前田英史

    日本歯科保存学会平成30年度秋季学術大会  2018.11 

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    Event date: 2018.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Effects of dopamine on odontoblastic differentiation. International conference

    Fujino S, Hamano S, Tomokiyo A, Hasegawa D, Yoshida Y, Sugii H, Washio A, Mitarai H, Nozu A, Arima M, Wada N, KitamuraC, Maeda H

    The IFEA 11th World Endodontic Congress 2018  2018.10 

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    Event date: 2018.10 - 2019.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  • Odontoblastic differentiation of senescence dental pulp cells treated by TNF-α. International conference

    2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (general)  

    Venue:London   Country:United Kingdom  

  • R-spondin2 Enhances Osteoblastic Differentiation of Immature Human Periodontal Ligament Cells. International conference

    Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H.

    The 96th General Session & Exhibition of the IADR.  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions. International conference

    Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Sonoda M, Nozu A, Wada N, Maeda H.

    The 96th General Session & Exhibition of the IADR  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Oral presentation (general)  

    Country:United Kingdom  

  • 象牙芽細胞分化に及ぼすドーパミンの影響について

    藤野翔香、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井秀樹、鷲尾絢子、御手洗裕美、野津葵、有馬麻衣、和田尚久、北村知昭、前田英史

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、野津葵、有馬麻衣、濱野さゆり、御手洗裕美、和田尚久、前田英史.

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Activin Aがヒト歯根膜細胞およびヒト前骨芽細胞の骨芽細胞様分化に及ぼす影響について

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、御手洗裕美、野津葵、有馬麻衣、 糸山知宏、小野太雅、藤野翔香、一法師啓太、和田尚久、前田英史.

    第148回日本歯科保存学会春季学術大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜みなとみらいホール   Country:Japan  

  • Basic Fibroblast Growth FactorおよびephrinB2がヒト歯根膜細胞の増殖に及ぼす影響について

    小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、御手洗裕美、有馬麻衣、野津葵、和田尚久、前田英史

    第148回日本歯科保存学会春季学術大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜みなとみらいホール   Country:Japan  

  • 老化したヒト歯髄細胞の象牙芽細胞様分化におよぼすTNF-alphaの影響について.第148回日本歯科保存学会春季学術大会, 第148回日本歯科保存学会春季学術大会, 2018.06.

    野津葵、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、御手洗裕美、一法師啓太、和田尚久、前田英史

    第148回日本歯科保存学会春季学術大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜みなとみらいホール   Country:Japan  

  • 新規幹細胞関連因子MESTがヒト歯根膜細胞の幹細胞転換に及ぼす影響

    長谷川大学、長谷川佳那、御手洗裕美、有馬麻衣、濱野さゆり、吉田晋一郎、友清淳、杉井英樹、和田尚久、清島保、前田英史.

    第148回日本歯科保存学会春季学術大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜みなとみらいホール   Country:Japan  

  • 歯根膜細胞におけるα-SMA発現にTransgelinが関与する

    御手洗 裕美, 和田 尚久, 前田 英史, 長谷川 大学, 吉田 晋一郎, 濱野 さゆり, 祐田 明香, 友清 淳, 赤峰 昭文

    第142回日本歯科保存学会春季学術大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:小倉   Country:Japan  

  • スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

    御手洗 裕美, Naati Fakatava, 王 恕心, 冉 子晴, 祐田 明香, 原口 晃, 孫 偉浩, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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    Language:Japanese  

  • IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

    王 恕心, 御手洗 裕美, 冉 子晴, 祐田 明香, 孫 偉浩, 原口 晃, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.10  (NPO)日本歯科保存学会

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  • ACTA2はTGF-β1の相互作用が有りと無しの機序を介してヒトPDL機能を調節する(ACTA2 regulates human PDL function via interaction with or without TGF-β1)

    Fakatava Naati, 御手洗 裕美, 祐田 明香, 原口 晃, 長谷川 大学, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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MISC

  • IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

    王 恕心, 御手洗 裕美, 冉 子晴, 祐田 明香, 孫 偉浩, 原口 晃, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   159回   140 - 140   2023.10

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    Language:Japanese   Publisher:(NPO)日本歯科保存学会  

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  • スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

    御手洗 裕美, Naati Fakatava, 王 恕心, 冉 子晴, 祐田 明香, 原口 晃, 孫 偉浩, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   158回   133 - 133   2023.5

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  • 患者情報やコミュニケーション技法を積極的に活用し歯科治療が可能となった1症例

    信太 実有, 御手洗 裕美, 和田 尚久

    日本総合歯科学会雑誌   14   39 - 46   2022.10   eISSN:2189-938X

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    定期的な口腔内清掃を希望する患者が来院するが,歯周疾患の治癒・病状安定を維持している患者が多い一方,病状が進行し予後不良の歯が出現しているものの,様々な理由で治療や抜歯を希望しない患者もいる。今回,患者情報を参考に,様々なコミュニケーション技法を応用して患者と治療方針を検討した。その結果,抜歯を含めた全顎的な治療に取り組むことができた症例を経験したため報告する。症例は76歳,男性。「右上の歯が痛い。」という主訴で来院した。患者は九州大学病院口腔総合診療科で全顎的治療を行った後にSupportive Periodontal Therapy(SPT)へ移行したが,その後約5年にわたり「簡単に掃除をやってほしい。」との訴えが続いた。そのため,定期的な口内法X線写真撮影や歯周基本検査を含む,十分なSPTを行えていなかった。そこで,過去の患者情報から患者の考え方を考察し,(1)目標設定理論を応用し,何か処置をする前には,処置内容,所要時間,その処置が必要な理由をその都度伝えること,(2)"But You Are Free"(BYAF) compliance-gaining techniqueを応用した質問形式を取ること,(3)"Self Persuasion"自主説得理論を応用した治療の提案を行うことのコミュニケーション技法を応用して治療を開始した。その結果,患者は現症や治療方法を十分理解・納得し,予後不良と判断した歯の抜歯と,上顎即時義歯を製作することができた。以上のことから,患者情報や様々なコミュニケーション技法を応用することで,治療へ移行ができる可能性があると考えられた。(著者抄録)

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  • ACTA2はTGF-β1の相互作用が有りと無しの機序を介してヒトPDL機能を調節する(ACTA2 regulates human PDL function via interaction with or without TGF-β1)

    Fakatava Naati, 御手洗 裕美, 祐田 明香, 原口 晃, 長谷川 大学, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   156回   29 - 29   2022.5

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Professional Memberships

  • 日本歯科保存学会

  • 日本顕微鏡歯科学会

  • 日本歯内療法学会

  • 大阪大学歯学会

  • International Association for Dental Research

Academic Activities

  • 実行委員

    顕微鏡歯科学会  ( オンライン ) 2021.4

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    Type:Competition, symposium, etc. 

    Number of participants:910

Research Projects

  • Elucidation of differentiation regulating mechanism by SPARC-related factors expressed in the dental papilla, and attempt of tooth regeneration

    Grant number:23K09140  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

    CiNii Research

  • 歯小嚢に発現するlncRNAを介したIGFシグナル応用新規歯周組織再生療法の樹立

    2022.4 - 2025.3

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    Authorship:Principal investigator 

  • 歯小嚢に発現するlncRNAを介したIGFシグナル応用新規歯周組織再生療法の樹立

    Grant number:22K17042  2022 - 2025

    日本学術振興会  科学研究費助成事業  若手研究

    御手洗 裕美

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    Authorship:Principal investigator  Grant type:Scientific research funding

    歯根膜細胞から、多分化能を有する歯根膜幹細胞へ効率的に誘導し、組織再生療法へ応用する方法を樹立するため、本研究では、歯根膜幹細胞の幹細胞特性に重要な因子を、歯根膜幹細胞を多く含む歯小嚢から探る予定である。我々の微量RNAシークエンス解析結果から歯小嚢に発現が高いことを明らかにしたIGF関連因子とlncRNA H19に着目し、in vivo、in vitroの両面から機能解析を行う。歯根膜幹細胞特性への関連も検討することで、IGF関連因子とH19による幹細胞特性の制御機構を明らかにし、より効率的に歯根膜幹細胞を誘導できる歯周組織再生療法を樹立する。

    CiNii Research

  • オミックス解析を用いた歯根膜発生機構の解明と幹細胞誘導型組織再生技術への応用

    Grant number:19H03832  2019.4 - 2023.3

    科学研究費助成事業  基盤研究(B)

    和田 尚久, 前田 英史, 友清 淳, 祐田 明香, 藤井 慎介, 御手洗 裕美, 和田 裕子

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    Grant type:Scientific research funding

    近年、歯周病などによって大きく喪失した歯周組織の新たな再生療法として、幹細胞移植によって歯根膜および歯槽骨の再生を積極的に促す方法が提案されているが実現に至っていない。本研究では、歯胚発生期に着目してオミックス解析により歯根膜発生・形成に重要な特異的因子を同定、機能解析することで歯根膜発生機構の一端を解明する。さらに歯根膜発生・形成に重要な同定因子を幹細胞分化制御候補因子として歯根膜細胞への分化誘導能についてin vitroおよびin vivo両面から機能解析を行い、歯根膜組織再生のための幹細胞分化制御機構を解明する。

    CiNii Research

  • アンキローシス発生機序探索の鍵となる新規TGF-βシグナル関連因子分子機構の解明

    2019.4 - 2022.3

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    Authorship:Principal investigator 

  • アンキローシス発生機序探索の鍵となる新規TGF-βシグナル関連因子分子機構の解明

    Grant number:19K19032  2019 - 2021

    日本学術振興会  科学研究費助成事業  若手研究

    御手洗 裕美

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    Authorship:Principal investigator  Grant type:Scientific research funding

    意図的再植後に起こりうるアンキローシスの発生をコントロールするため、予知性の高い治療法への改善が必要とされている。本研究では歯根膜組織の緩衝能に重要なTGF-β-α-SMAシグナルの新規発現制御因子の機能ならびに分子基盤の詳細な解析と、エピジェネティックな転写制御機能を明らかにしたうえで、エピジェネティクス関連分子を用いたin vivoモデル(意図的再植)における歯根膜組織再生効果を検討する。本研究から得られる結果により、咬合力による緩衝能を維持した歯根膜細胞を増殖・誘導し、アンキローシスを防止して意図的再植法の予知性を高める新たな歯内疾患治療法の開発に繋げていくことができると考えられる。

    CiNii Research

  • 歯根膜幹細胞誘導因子の同定と新規歯周組織再生療法の開発

    2018.6

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    Authorship:Principal investigator 

  • 歯根膜幹細胞誘導因子の同定と新規歯周組織再生療法の開発

    Grant number:17H06953  2017 - 2018

    科学研究費助成事業  若手研究(スタートアップ)

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Class subject

  • リサーチエクスポージャー

    2020.4 - 2020.9   First semester

  • リサーチエクスポージャー

    2019.4 - 2020.3   Full year

  • リサーチエクスポージャー

    2018.4 - 2018.9   First semester

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2024  九州医療専門学校  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2022  九州医療専門学校  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2021  九州医療専門学校  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2020  九州医療専門学校  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2019  九州医療専門学校  Classification:Intensive course  Domestic/International Classification:Japan 

Outline of Social Contribution and International Cooperation activities

  • ・研究で得られた知見を、学会において国民に発信する。
    ・地域歯科医療をサポートする。

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

Clinician qualification

  • Certifying physician

    日本歯科保存学会

Year of medical license acquisition

  • 2012

Notable Clinical Activities

  • 歯内治療・修復治療を専門とした歯科治療