Faculty Profiles - MITARAI HIROMI

Information

写真a

MITARAI HIROMI

Organization

Kyushu University Hospital General Dentistry Assistant Professor
Abolition organization Comprehensive Dentistry（Joint Appointment）
School of Dentistry Department of Dentistry（Joint Appointment）
Graduate School of Dental Science （Joint Appointment）
Graduate School of Dental Science Department of Dental Science（Joint Appointment）

Profile

研究：根尖性歯周炎、歯根破折や歯周病によって失われた歯根膜組織再生を目指して研究を行っている。特に、ヒト歯根膜細胞に幹細胞特性を付与する因子の検討・解析を行っている。また、細菌感染によって惹起された歯髄炎に対し、歯髄炎抑制効果ならびに抗菌作用のある因子を探索・解析を行っている。教育：歯科医師の卒後研修指導、大学院生ならびに学部学生の研究指導を担当している。臨床：口腔総合診療を行っている。特に歯内治療、修復治療を専門に行っている。

Homepage

http://www.kososhin.hosp.kyushu-u.ac.jp/
Division of General Dentistry, Kyushu University Hospital

External link

Degree

Doctor of Dental Science

Research Interests・Research Keywords

Research theme：Development of new periodontal ligament regeneration therapy

Keyword：periodontal ligament, stem cell, exosome

Research period： 2018.6
Research theme：The effect of Transgelin on homeostasis of human periodontal ligament cells

Keyword：human periodontal ligament cells

Research period： 2013.4 - 2017.3

Papers

Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Reviewed International journal

2023.4

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Background/purpose: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells.

Materials and methods: RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells.

Results: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2-23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2-23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1-stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3.

Conclusion: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies.
患者情報やコミュニケーション技法を積極的に活用し歯科治療が可能となった 1 症例 Reviewed

信太実有, 御手洗裕美, 和田尚久

2022.12

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Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells. Reviewed International journal

52 ( 6 ) 984 - 993 2017.12

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BACKGROUND AND OBJECTIVE:
Human periodontal ligament cells (HPDLCs) express transforming growth factor-β1 (TGF-β1) that regulates differentiation and proliferation, and plays key roles in homeostasis of PDL tissue. Transgelin is a cytoskeleton-associated protein with an Smad-binding element in its gene promoter region. In this study, we examined the localization and potential function of transgelin in PDL tissue and cells.

MATERIAL AND METHODS:
Microarray analysis of HPDLC lines (2-14, 2-23 and 2-52) was performed. Expression of transgelin in HPDLCs was examined by quantitative reverse transcription-polymerase chain reaction, immunofluorescence staining and western blot analysis. Effects of TGF-β1 and its signaling inhibitor, SB431542, on transgelin expression in HPDLCs were examined by western blot analysis. The effects of transgelin knockdown by small interfering RNA (siRNA) on HPDLC proliferation stimulated by TGF-β1 were assessed by WST-1 assay.

RESULTS:
In microarray and quantitative reverse transcription-polymerase chain reaction analyses, the expression levels of transgelin (TAGLN) in 2-14 and 2-23 cells, which highly expressed PDL markers such as periostin (POSTN), tissue non-specific alkaline phosphatase (ALPL), α-smooth muscle actin (ACTA2) and type I collagen A1 (COL1A1), was significantly higher than those in 2-52 cells that expressed PDL markers weakly. Immunohistochemical and immunofluorescence staining revealed expression of transgelin in rat PDL tissue and HPDLCs. In HPDLCs, TGF-β1 treatment upregulated transgelin expression, whereas inhibition of the type 1 TGF-β1 receptor by SB431542 suppressed this upregulation. Furthermore, TAGLN siRNA transfection did not promote the proliferation of HPDLCs treated with TGF-β1. The expression levels of CCNA2 and CCNE1, which regulate DNA synthesis and mitosis through the cell cycle, were also not upregulated in HPDLCs transfected with TAGLN siRNA.

CONCLUSION:
Transgelin is expressed in PDL tissue and might have a role in HPDLC proliferation induced by TGF-β1 stimulation.

DOI： 10.1111/jre.12466
In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria Reviewed International journal

Harunobu Kamiya, Akira Haraguchi, Hiromi Mitarai, Asuka Yuda, Hiroko Wada, Wang Shuxin, Ran Ziqing, Sun Weihao, Naohisa Wada

Journal of Infection and Chemotherapy 2024.4

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MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells Reviewed International journal

@Daigaku Hasegawa,@Kana Hasegawa,#Hiroshi Kaneko,@Shinichiro Yoshida,@Hiromi Mitarai,@Mai Arima,@Atsushi Tomokiyo,@Sayuri Hamano, @Hideki Sugii,@Naohisa Wada,@Tamotsu Kiyoshima,@Hidefumi Maeda

Hindawi 2020.7

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Functions of beta2-adrenergic receptor in human periodontal ligament cells Invited Reviewed International journal

Sayuri Hamano Atsushi Tomokiyo Daigaku Hasegawa Asuka Yuda Hideki Sugii Shinichiro Yoshida Hiromi Mitarai Naohisa Wada Hidefumi Maeda

Journal of Cellular Biochemistry 2020.7

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R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/beta-catenin signaling pathway. Reviewed International journal

Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H

J Periodont Res. 54 ( 2 ) 143 - 153 2019.4

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DOI： 10.1111/jre.12611
骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する

吉田晋一郎、和田尚久、長谷川大学、御手洗裕美、有馬麻衣、友清　淳、濱野さゆり、杉井英樹

日歯保存誌 61 ( 6 ) 343 - 353 2018.12

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Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling. Reviewed International journal

Hasegawa D, Wada N, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Maeda H.

J Cell Physiol. 2018.2

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Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling Reviewed

Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda

Journal of Cellular Physiology 233 ( 2 ) 1752 - 1762 2018.2

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DOI： 10.1002/jcp.26086
Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells Reviewed

Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Shoko Fujino, Naohisa Wada, Hidefumi Maeda

Stem Cells and Development 27 ( 2 ) 100 - 111 2018.1

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DOI： 10.1089/scd.2017.0077
Senescence and odontoblastic differentiation of dental pulp cells. Reviewed International journal

Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H.

J Cell Physiol. 234 ( 1 ) 849 - 859 2018.1

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DOI： 10.1002/jcp.26905
Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells. Reviewed International journal

Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H.

Stem Cells Dev. 2018.1

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Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells. Reviewed International journal

Mizumachi H, Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Yuda A, Sugii H, Serita S, Mitarai H, Koori K, Wada N, Maeda H.

Bone 2017.8

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Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells Reviewed

Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda

Bone 101 191 - 201 2017.8

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DOI： 10.1016/j.bone.2017.05.012
Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells Reviewed

78 135 - 143 2017.6

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Objective The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). Design A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. Results Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. Conclusions The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.

DOI： 10.1016/j.archoralbio.2017.02.018
GDNF From Human Periodontal Ligament Cells Treated With Pro-Inflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells Reviewed

Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Satoshi Monnouchi, Asuka Yuda, Hidefumi Maeda

Journal of Cellular Biochemistry 118 ( 4 ) 699 - 708 2017.4

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DOI： 10.1002/jcb.25662
Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells. Reviewed International journal

2017.2

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OBJECTIVE:

The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs).
DESIGN:

A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR.
RESULTS:

Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs.
CONCLUSIONS:

The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.
Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells Reviewed

Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, H. Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

Journal of Dental Research 95 ( 11 ) 1282 - 1290 2016.10

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DOI： 10.1177/0022034516653085
Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. Invited Reviewed International journal

Yoshida S, Wada N, Hasegawa D, Miyaji H, Mitarai H, Tomokiyo A, Hamano S, Maeda H.

J Dent Res 2016.10

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Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression. Reviewed International journal

2015.11

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Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and Western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression.
Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression Reviewed

230 ( 11 ) 2647 - 2660 2015.11

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Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and Western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression.

DOI： 10.1002/jcp.24950

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Presentations

スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

御手洗裕美、Naati Fakatava、王恕心、冉子晴、祐田明香、原口晃、孫偉浩、和田尚久

日本歯科保存学会2023年度春季大会（第158回） 2023.6

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Event date： 2023.6

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：くにびきメッセ Country：Japan
術前の口腔内診査と診断の重要性を学んだ2つの症例 Invited

御手洗　裕美

第15回日本総合歯科学会学術大会 2022.11

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Event date： 2022.11 - 2023.11

Language：Japanese Presentation type：Symposium, workshop panel (public)

Country：Japan
The Role of Acta2 in Periodontal Ligament cell stimulated with TGF-β1

Fakatava Naati、御手洗裕美、祐田明香、長谷川大学、前田英史、和田尚久

日本歯科保存学会 2020.6

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Event date： 2020.6

Language：English

Venue：Web開催 Country：Japan
Transgelinは、Integrinを介した細胞外基質への接着に関与する

御手洗裕美、祐田明香、Fakatava Naati、長谷川大学、前田英史、和田尚久

日本歯科保存学会 2020.6

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Event date： 2020.6

Language：Japanese

Venue：Web開催 Country：Japan
IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

王恕心、御手洗裕美、冉子晴、祐田明香、孫偉浩、原口晃、前田英史、和田尚久

日本歯科保存学会2023年度秋季大会（第159回） 2023.11

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Event date： 2023.11

Language：English Presentation type：Symposium, workshop panel (public)

Venue：アクトシティ浜松 Country：Japan
著しい骨隆起を有する患者に対し包括的治療を行った症例

渡邉護煕，御手洗裕美，王丸寛美，和田尚久

第 16 回日本総合歯科学会学術大会 2023.10

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Event date： 2023.10

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：日本大学歯学部創設百周年記念講堂 Country：Japan
ACTA2 regulates human PDL function via interaction with or without TGF-β1

Naati Fakatava, 御手洗　裕美, 祐田　明香, 原口　晃、長谷川　大学、前田　英史、和田　尚久

日本歯科保存学会2022年度春季学術大会（第156回） 2022.6

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Event date： 2022.6

Language：English Presentation type：Oral presentation (general)

Venue：Web開催 Country：Japan

[OBJECTIVE] ACTA2 (alpha-smooth muscle actin; α-SMA), one of the cytoskeleton protein, is known to be expressed in periodontal ligament (PDL) tissue. It might be involved in PDL function such as proliferation and migration. Also ACTA2 is upregulated with transforming growth factor-β1 (TGF-β1) which exists in PDL tissue predominantly (Fujii et al, Cell Tissue Res. 2010). So it is assumed that ACTA2 is involved in TGF-β1-dependent function in human PDL cells, but little is known about them. Therefore, we focused on ACTA2 and examined its role within the PDL function via interaction with or without TGF-β1 to find its role in the remodeling of the PDL.
[MATERIALS AND METHODS] Human PDL cell line 2-23, which was isolated from heterogeneous immortalized Human PDL cells (Hasegawa et al. J Cell Physiol. 2018) was used. (1) Western blotting analysis was performed to examine the protein expression of ACTA2. (2) ACTA2 or scramble siRNA were transfected to 2-23 for 48 h, and performed the WST-1 assay and scratch wound healing assay to analyze the proliferation and migration. (3) 2-23 cells were cultured with or without human recombinant TGF-β1 (rhTGF-β1: 10 ng/ml) for 24 h in the presence of ACTA2 or scramble siRNA. qRT-PCR was performed to examined the mRNA expression of PDL related genes; collagen1A1 (COL1A1), periostin (POSTN), and fibrillin1 (FBN-1). Picro-sirius red staining and sircol collagen assay were performed to analyze the collagen production. Western blotting analysis was performed to examine the phosphorylation of TGF-β1-related molecules; Smad2, Smad3, and YAP. All procedures were performed in compliance with requirements of the Institutional Review Board for Human Genome / Gene Research (approval number: 30-167) and Research Ethics Committee (approval number: 27-76) at Kyushu University.
[RESULTS] ACTA2 protein expression was observed through Western blotting analysis in 2-23. After transfection with ACTA2 siRNA, cell proliferation and migration levels were significantly downregulated compared with scramble siRNA. The mRNA expression of ACTA2, COL1A1, POSTN, and FBN-1 was upregulated in 2-23 stimulated with TGF-β1. Those mRNA expression was significantly downregulated in the presence of ACTA2 siRNA stimulated with rhTGF-β1 compared with scramble siRNA. The amounts of collagen production were upregulated in 2-23 stimulated with rhTGF-β1 which were analyzed by picro-sirius red staining and sircol collagen assay. But after ACTA2 knockdown, the collagen production stimulated with rhTGF-β1 were significantly downregulated compared with scramble siRNA. We revealed that phosphorylation of Smad2 and Smad3 in 2-23 were observed at 15-min time point with TGF-β1, and phosphorylation of YAP was observed at 30-min time point with TGF-β1 by Western blotting analysis. However, after ACTA2 knockdown, at each time point, the phosphorylation of Smad2, Smad3, and YAP was downregulated after TGF-β1 stimulation.
[DISCUSSION] In this research, ACTA2 was involved in proliferation and migration of human PDL cells. These results suggest that as cytoskeleton protein, ACAT2 itself is crucial for PDL function. In the presence of ACTA2 siRNA, upregulation of PDL related genes, collagen production, and phosphorylation of TGF-β1-related molecules were significantly downregulated, suggesting that ACTA2 might be a key factor for TGF-β1 function.
[CONCLUSION] ACTA2 regulates human PDL function via interaction with or without TGF-β1.
患者とのラポール形成で、抜歯即時義歯を製作できた１例

信太実有、御手洗裕美、和田尚久

第14回日本総合歯科学会総会・学術大会 2021.10

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Event date： 2021.10 - 2021.11

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：Web開催 Country：Japan
Terpinen-4-ol の根管内細菌に対する抗菌性の検討

神谷治伸、原口晃、御手洗裕美、Fakatava Naati、祐田明香、前田英史、和田尚久

日本歯科保存学会2022年度春季学術大会（第155回） 2021.10

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Event date： 2021.10 - 2021.11

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：Web開催 Country：Japan
Effects of TNF-alpha on Senescent human dental pulp cells. Invited

Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda.

Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2019.3

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Event date： 2019.3

Language：English Presentation type：Symposium, workshop panel (public)

Country：Japan
LGR4が未分化なヒト歯根膜細胞の増殖能，走化性および骨芽細胞様分化に及ぼす影響

有馬麻衣，長谷川大学，吉田晋一郎，御手洗裕美，友清淳，濱野さゆり，杉井英樹，和田尚久，前田英史

日本歯科保存学会平成30年度秋季学術大会 2018.11

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Event date： 2018.11

Language：Japanese Presentation type：Oral presentation (general)

Country：Japan
Effects of dopamine on odontoblastic differentiation. International conference

Fujino S, Hamano S, Tomokiyo A, Hasegawa D, Yoshida Y, Sugii H, Washio A, Mitarai H, Nozu A, Arima M, Wada N, KitamuraC, Maeda H

The IFEA 11th World Endodontic Congress 2018 2018.10

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Event date： 2018.10 - 2019.10

Language：English Presentation type：Oral presentation (general)

Country：Korea, Republic of
Odontoblastic differentiation of senescence dental pulp cells treated by TNF-α. International conference

2018.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Venue：London Country：United Kingdom
R-spondin2 Enhances Osteoblastic Differentiation of Immature Human Periodontal Ligament Cells. International conference

Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H.

The 96th General Session & Exhibition of the IADR. 2018.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Country：United Kingdom
Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions. International conference

Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Sonoda M, Nozu A, Wada N, Maeda H.

The 96th General Session & Exhibition of the IADR 2018.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Country：United Kingdom
象牙芽細胞分化に及ぼすドーパミンの影響について

藤野翔香、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井秀樹、鷲尾絢子、御手洗裕美、野津葵、有馬麻衣、和田尚久、北村知昭、前田英史

第39回日本歯内療法学会学術大会 2018.7

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Event date： 2018.7

Language：Japanese Presentation type：Oral presentation (general)

Country：Japan
Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、野津葵、有馬麻衣、濱野さゆり、御手洗裕美、和田尚久、前田英史．

第39回日本歯内療法学会学術大会 2018.7

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Event date： 2018.7

Language：Japanese Presentation type：Oral presentation (general)

Country：Japan
Activin Aがヒト歯根膜細胞およびヒト前骨芽細胞の骨芽細胞様分化に及ぼす影響について

杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、御手洗裕美、野津葵、有馬麻衣、糸山知宏、小野太雅、藤野翔香、一法師啓太、和田尚久、前田英史．

第148回日本歯科保存学会春季学術大会 2018.6

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Event date： 2018.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：横浜みなとみらいホール Country：Japan
Basic Fibroblast Growth FactorおよびephrinB2がヒト歯根膜細胞の増殖に及ぼす影響について

小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、御手洗裕美、有馬麻衣、野津葵、和田尚久、前田英史

第148回日本歯科保存学会春季学術大会 2018.6

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Event date： 2018.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：横浜みなとみらいホール Country：Japan
老化したヒト歯髄細胞の象牙芽細胞様分化におよぼすTNF-alphaの影響について．第148回日本歯科保存学会春季学術大会, 第148回日本歯科保存学会春季学術大会, 2018.06.

野津葵、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、御手洗裕美、一法師啓太、和田尚久、前田英史

第148回日本歯科保存学会春季学術大会 2018.6

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Event date： 2018.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：横浜みなとみらいホール Country：Japan
新規幹細胞関連因子MESTがヒト歯根膜細胞の幹細胞転換に及ぼす影響

長谷川大学、長谷川佳那、御手洗裕美、有馬麻衣、濱野さゆり、吉田晋一郎、友清淳、杉井英樹、和田尚久、清島保、前田英史．

第148回日本歯科保存学会春季学術大会 2018.6

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Event date： 2018.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：横浜みなとみらいホール Country：Japan
_{歯根膜細胞におけるα-SMA発現にTransgelinが関与する}

御手洗　裕美, 和田尚久, 前田英史, 長谷川大学, 吉田　晋一郎, 濱野さゆり, 祐田　明香, 友清淳, 赤峰昭文

第142回日本歯科保存学会春季学術大会 2015.6

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Event date： 2015.6

Language：Japanese Presentation type：Symposium, workshop panel (public)

Venue：小倉 Country：Japan

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Professional Memberships

日本歯科保存学会
日本顕微鏡歯科学会
日本歯内療法学会
大阪大学歯学会
International Association for Dental Research

Academic Activities

実行委員

顕微鏡歯科学会（オンライン） 2021.4

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Type：Competition, symposium, etc.

Number of participants：910

Research Projects

歯小嚢に発現するlncRNAを介したIGFシグナル応用新規歯周組織再生療法の樹立

2022.4 - 2025.3

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Authorship：Principal investigator
歯小嚢に発現するlncRNAを介したIGFシグナル応用新規歯周組織再生療法の樹立

Grant number：22K17042 2022 - 2025

日本学術振興会科学研究費助成事業若手研究

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Authorship：Principal investigator Grant type：Scientific research funding
アンキローシス発生機序探索の鍵となる新規TGF-βシグナル関連因子分子機構の解明

2019.4 - 2022.3

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Authorship：Principal investigator
アンキローシス発生機序探索の鍵となる新規TGF-βシグナル関連因子分子機構の解明

Grant number：19K19032 2019 - 2021

日本学術振興会科学研究費助成事業若手研究

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Authorship：Principal investigator Grant type：Scientific research funding
歯根膜幹細胞誘導因子の同定と新規歯周組織再生療法の開発

2018.6

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Authorship：Principal investigator
歯根膜幹細胞誘導因子の同定と新規歯周組織再生療法の開発

Grant number：17H06953 2017 - 2018

科学研究費助成事業若手研究(スタートアップ)

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Authorship：Principal investigator Grant type：Scientific research funding

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Class subject

リサーチエクスポージャー

2020.4 - 2020.9 First semester
リサーチエクスポージャー

2019.4 - 2020.3 Full year
リサーチエクスポージャー

2018.4 - 2018.9 First semester

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

2022 九州医療専門学校 Classification:Intensive course Domestic/International Classification:Japan
2021 九州医療専門学校 Classification:Intensive course Domestic/International Classification:Japan
2020 九州医療専門学校 Classification:Intensive course Domestic/International Classification:Japan
2019 九州医療専門学校 Classification:Intensive course Domestic/International Classification:Japan

Outline of Social Contribution and International Cooperation activities

・研究で得られた知見を、学会において国民に発信する。
・地域歯科医療をサポートする。

Specialized clinical area

Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

Clinician qualification

Certifying physician

日本歯科保存学会

Year of medical license acquisition

2012

Notable Clinical Activities

歯内治療・修復治療を専門とした歯科治療