2025/06/18 更新

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写真a

ドイ ゴロウ
土井 吾郎
DOI GORO
所属
九州大学病院別府病院 内科 助教
職名
助教

研究分野

  • ライフサイエンス / 免疫学

学位

  • 医学博士 ( 九州大学 )

経歴

  • 九州大学 九州大学病院別府病院 内科  助教 

    2024年4月 - 現在

学歴

  • 九州大学   医学部   医学科

    2007年4月 - 2013年3月

研究テーマ・研究キーワード

  • 研究テーマ: 免疫学

    研究キーワード: 膠原病

    研究期間: 2023年4月

論文

  • Strategic targeting of Cas9 nickase expands tandem gene arrays

    武居 宏明, 岡田 悟, 土井 吾郎, 杉山 友貴, 楠元 恵美子, 伊藤 隆司

    Cell Genomics   5 ( 4 )   100811   2025年4月   ISSN:2666979X

     詳細を見る

    記述言語:英語   出版者・発行元:Elsevier  

    Expanding tandem gene arrays facilitates adaptation through dosage effects and gene family formation via sequence diversification. However, experimental induction of such expansions remains challenging. Here, we introduce a method termed break-induced replication (BIR)-mediated tandem repeat expansion (BITREx) to address this challenge. BITREx places Cas9 nickase adjacent to a tandem gene array to break the replication fork that has just replicated the array, forming a single-ended double-strand break. This break is subsequently end-resected to become single stranded. Since there is no repeat unit downstream of the break, the single-stranded DNA often invades an upstream unit to initiate ectopic BIR, resulting in array expansion. BITREx has successfully expanded gene arrays in budding yeast, with the CUP1 array reaching ∼1 Mb. Furthermore, appropriate splint DNAs allow BITREx to generate tandem arrays de novo from single-copy genes. We have also demonstrated BITREx in mammalian cells. Therefore, BITREx will find various unique applications in genome engineering.

    CiNii Research

  • Strategic targeting of Cas9 nickase expands tandem gene arrays

    Takesue, H; Okada, S; Doi, G; Sugiyama, Y; Kusumoto, E; Ito, T

    CELL GENOMICS   5 ( 4 )   100811   2025年4月   ISSN:2666-979X

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    記述言語:英語   出版者・発行元:Cell Genomics  

    Expanding tandem gene arrays facilitates adaptation through dosage effects and gene family formation via sequence diversification. However, experimental induction of such expansions remains challenging. Here, we introduce a method termed break-induced replication (BIR)-mediated tandem repeat expansion (BITREx) to address this challenge. BITREx places Cas9 nickase adjacent to a tandem gene array to break the replication fork that has just replicated the array, forming a single-ended double-strand break. This break is subsequently end-resected to become single stranded. Since there is no repeat unit downstream of the break, the single-stranded DNA often invades an upstream unit to initiate ectopic BIR, resulting in array expansion. BITREx has successfully expanded gene arrays in budding yeast, with the CUP1 array reaching ∼1 Mb. Furthermore, appropriate splint DNAs allow BITREx to generate tandem arrays de novo from single-copy genes. We have also demonstrated BITREx in mammalian cells. Therefore, BITREx will find various unique applications in genome engineering.

    DOI: 10.1016/j.xgen.2025.100811

    Web of Science

    Scopus

    PubMed

  • Successful control of recurrent MAS by canakinumab in a Sjögren syndrome patient with homozygous MEFV P369S variants and review of literatures

    Ono, N; Yoshimura, M; Nishida, T; Yamauchi, Y; Doi, G; Fuyuno, Y; Sonoda, M; Niiro, H

    MODERN RHEUMATOLOGY CASE REPORTS   2025年3月   eISSN:2472-5625

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  • Comparative efficacy and safety of anifrolumab for belimumab-experienced and biologic-naïve patients with systemic lupus erythematosus: Results from the Kyushu Collagen Disease Network for Systemic Lupus Erythematosus (KCDN-SLE) registry

    Ayano, M; Ueda, N; Mishima, K; Ota, SI; Kushimoto, K; Tanaka, A; Doi, G; Nakayama, T; Maekawa, M; Tsuru, T; Akahoshi, M; Akashi, K; Horiuchi, T; Niiro, H

    JOINT BONE SPINE   92 ( 4 )   2025年3月   ISSN:1297-319X eISSN:1778-7254

  • Treatment selection in the clinical practice of systemic lupus erythematosus: Results from the Kyushu Collagen Disease Network for Systemic Lupus Erythematosus(KCDN-SLE) registry(タイトル和訳中)

    Ayano Masahiro, Ueda Naoyasu, Mishima Koji, Ota Shun-Ichiro, Kushimoto Kazuo, Tanaka Atsushi, Kawano Shotaro, Nishimura Naoya, Kashiwado Yusuke, Doi Goro, Nakayama Tsuyoshi, Fukumoto Ryo, Tsuru Tomomi, Suzaki Midori, Akahoshi Mitsuteru, Maekawa Makiko, Omoto Aya, Tada Hitoshi, Akashi Koichi, Horiuchi Takahiko, Niiro Hiroaki

    Modern Rheumatology   35 ( 1 )   102 - 109   2025年1月   ISSN:1439-7595

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    記述言語:英語   出版者・発行元:Oxford University Press  

  • Systematic review and meta-analysis for the 2024 update of the Japan College of Rheumatology clinical practice guidelines for the management of rheumatoid arthritis(タイトル和訳中)

    Nakayama Yoichi, Nagata Wataru, Takeuchi Yoichi, Fukui Sho, Fujita Yuya, Hosokawa Yohei, Ueno Masanobu, Ono Kumiko, Sumitomo Shuji, Tabuchi Yuya, Nakanishi Yuichiro, Saito Shuntaro, Ikeuchi Hiroko, Kawamori Kazutaka, Sofue Hideaki, Doi Goro, Minami Runa, Hirota Tomoya, Minegishi Kaoru, Maeshima Keisuke, Motoyama Ryo, Nakamura Shohei, Suzuki Shotaro, Nishioka Norihiro, Tsuzuki Wada Takuma, Onishi Akira, Nishimura Kenichi, Watanabe Ryu, Yanai Ryo, Kida Takashi, Nishiwaki Hiroki, Yajima Nobuyuki, Kaneko Yuko, Tanaka Eiichi, Kawahito Yutaka, Harigai Masayoshi

    Modern Rheumatology   34 ( 6 )   1079 - 1094   2024年11月   ISSN:1439-7595

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    記述言語:英語   出版者・発行元:Oxford University Press  

  • Treatment selection in the clinical practice of systemic lupus erythematosus: Results from the Kyushu Collagen Disease Network for Systemic Lupus Erythematosus (KCDN-SLE) registry

    Ayano, M; Ueda, N; Mishima, K; Ota, SI; Kushimoto, K; Tanaka, A; Kawano, S; Nishimura, N; Kashiwado, Y; Doi, G; Nakayama, T; Fukumoto, R; Tsuru, T; Suzaki, M; Akahoshi, M; Maekawa, M; Omoto, A; Tada, H; Akashi, K; Horiuchi, T; Niiro, H

    MODERN RHEUMATOLOGY   35 ( 1 )   102 - 109   2024年8月   ISSN:1439-7595 eISSN:1439-7609

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    記述言語:英語   出版者・発行元:Modern Rheumatology  

    Objectives: This study aimed to describe the treatment selection for systemic lupus erythematosus (SLE) using data from the Kyushu Collagen Disease Network for SLE (KCDN-SLE) registry, a multicentre prospective registry in Japan. Methods: This study used data from patients registered between August 2022 and November 2023. Clinical characteristics, purpose of agent initiation, other candidate agents, and short-term efficacy and safety were evaluated. Results: We analysed 69 previously treated patients with SLE (mean age 43.7 years; 62 females, 7 males). Hydroxychloroquine, biological agents, and immunosuppressive agents were initiated during the maintenance phase in 12, 41, and 16 patients, respectively. In patients with active organ involvement, hydroxychloroquine and biological agents were widely used for initiation. In those who already achieved treatment goals, biological agents alone were predominantly selected. The SLE Disease Activity Index 2000 score and prednisolone dose declined significantly over a 6-month follow-up period. Among 48 patients with active disease, 22 achieved a lupus low disease activity state, but this had no evident association with the initiation of a biological agent. In total, 14 adverse events, predominantly infections, were observed. Conclusions: Biological agents were used preferentially, and the therapeutic agents were appropriately effective and mostly achieved the purpose of agent initiation.

    DOI: 10.1093/mr/roae058

    Web of Science

    Scopus

    PubMed

  • Systematic review and meta-analysis for the 2024 update of the Japan College of Rheumatology clinical practice guidelines for the management of rheumatoid arthritis

    Nakayama, Y; Nagata, W; Takeuchi, Y; Fukui, S; Fujita, Y; Hosokawa, Y; Ueno, M; Ono, K; Sumitomo, S; Tabuchi, Y; Nakanishi, Y; Saito, S; Ikeuchi, H; Kawamori, K; Sofue, H; Doi, G; Minami, R; Hirota, T; Minegishi, K; Maeshima, K; Motoyama, R; Nakamura, S; Suzuki, S; Nishioka, N; Wada, TT; Onishi, A; Nishimura, K; Watanabe, R; Yanai, R; Kida, T; Nishiwaki, H; Yajima, N; Kaneko, Y; Tanaka, E; Kawahito, Y; Harigai, M

    MODERN RHEUMATOLOGY   34 ( 6 )   1079 - 1094   2024年6月   ISSN:1439-7595 eISSN:1439-7609

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    記述言語:英語   出版者・発行元:Modern Rheumatology  

    Objectives: The aim of this article is to update evidence on the efficacy and safety of disease-modifying antirheumatic drugs (DMARDs) and provide information to the taskforce for the 2024 update of the Japan College of Rheumatology clinical practice guidelines for the management of rheumatoid arthritis (RA). Methods: We searched various databases for randomised controlled trials on RA published until June 2022, with no language restriction. For each of the 15 clinical questions, two independent reviewers screened the articles, evaluated the core outcomes, and performed meta-analyses. Results: Subcutaneous injection of methotrexate (MTX) showed similar efficacy to oral MTX in MTX-naïve RA patients. Ozoralizumab combined with MTX improved drug efficacy compared to the placebo in RA patients with inadequate response (IR) to conventional synthetic DMARD (csDMARD). Rituximab with and without concomitant csDMARDs showed similar efficacy to other biological DMARDs (bDMARDs) in bDMARD-IR RA patients. Combined Janus kinase inhibitors and MTX achieved similar clinical responses and equal safety during a 4-year period compared to tumour necrosis factor inhibitors in MTX-IR RA patients. Biosimilars showed efficacy equivalent to that of the original bDMARDs in csDMARD-IR and bDMARD-IR RA patients. Conclusions: This systematic review provides latest evidence for the 2024 update of the Japan College of Rheumatology clinical practice guidelines for RA management.

    DOI: 10.1093/mr/roae049

    Web of Science

    Scopus

    PubMed

  • Immunosuppressive therapy and humoral response to third mRNA COVID-19 vaccination with a six-month interval in rheumatic disease patients

    Kashiwado, Y; Kimoto, Y; Ohshima, S; Sawabe, T; Irino, K; Nakano, S; Hiura, J; Yonekawa, A; Wang, QL; Doi, G; Ayano, M; Mitoma, H; Ono, N; Arinobu, Y; Niiro, H; Hotta, T; Kang, D; Shimono, N; Akashi, K; Takeuchi, T; Horiuchi, T

    RHEUMATOLOGY   63 ( 3 )   725 - 733   2024年3月   ISSN:1462-0324 eISSN:1462-0332

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    記述言語:英語   出版者・発行元:Rheumatology (United Kingdom)  

    Objectives: To evaluate the long-term impact of immunosuppressive therapeutic agents on antibody response to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) mRNA vaccination in patients with autoimmune rheumatic diseases (AIRD) in order to propose a strategy for annual vaccination. Methods: This prospective multicentre cohort study evaluated the humoral response to second and third BNT162b2 and/or mRNA-1273 vaccines in 382 Japanese AIRD patients classified into 12 different medication groups and in 326 healthy controls (HCs). The third vaccination was administered six months after the second vaccination. Antibody titres were measured using the Elecsys Anti-SARS-CoV-2 S assay. Results: The seroconversion rate and antibody titres were lower in AIRD patients than in HCs 3–6 weeks after the second vaccination and 3–6 weeks after the third vaccination. Seroconversion rates were <90% after the third vaccination in patients receiving mycophenolate mofetil and rituximab. Antibody levels after the third vaccination were significantly lower in the groups prescribed TNF inhibitor with or without methotrexate, abatacept and rituximab or cyclophosphamide than those of HCs in a multivariate analysis adjusting for age, sex, and glucocorticoid dosage. The third vaccination induced an adequate humoral response in patients treated with sulfasalazine, bucillamine, methotrexate monotherapy, iguratimod, interleukin-6 inhibitors or calcineurin inhibitors including tacrolimus. Conclusions: Repeated vaccinations in many immunosuppressed patients produced antibody responses similar to those observed in HCs. In contrast, annual vaccination in patients receiving TNF inhibitors, abatacept, mycophenolate mofetil and rituximab may require caution.

    DOI: 10.1093/rheumatology/kead275

    Web of Science

    Scopus

    PubMed

▼全件表示

講演・口頭発表等

▼全件表示

MISC

共同研究・競争的資金等の研究課題

  • 抗MDA5抗体陽性皮膚筋炎における末梢血単球のメチローム及びセルフリーDNA解析

    研究課題/領域番号:23K15351  2023年4月 - 2027年3月

    科学研究費助成事業  若手研究

    土井 吾郎

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    資金種別:科研費

    抗MDA5抗体陽性皮膚筋炎は急速進行性間質性肺炎の合併により重篤な転帰をとる炎症性筋疾患である。単球などの自然免疫の関与を疑い、末梢血単球とcell-free DNA (cfDNA)に着目しエピゲノムを含めた多角的な解析を行う。疾患活動性に関与する単球のサブセットを同定し、単球のメチローム及びトランスクリプトームからエピゲノムレベルの特性を明らかにする。cfDNAについては少量の血清からシーケンシングが可能な独自のライブラリ作成技術を用いて質的な評価も試みる。疾患活動性や予後予測に関する新規バイオマーカーの探索を行い、既存治療の最適化や、単球を標的とした新規治療薬の開発につながる研究である。

    CiNii Research

専門診療領域

  • 生物系/医歯薬学/内科系臨床医学/膠原病・アレルギー・感染症内科学

臨床医資格

  • 専門医

    日本内科学会

  • 専門医

    日本リウマチ学会