Authorship：Lead author,　Corresponding author  

DOI： https://doi.org/10.1038/s41586-020-2926-0

Language：English  

DOI： 10.1101/2023.04.09.536178

PubMed

Authorship：Corresponding author   Language：English   Publisher：Nature Chemical Biology  

Identifying the protein component of calcium signaling in specific subcellular regions presents considerable challenges. Researchers have now successfully integrated calcium indicators with proximity labeling, enabling the targeting of microdomain-specific calcium signaling.

DOI： 10.1038/s41589-024-01656-8

Web of Science

Scopus

PubMed

Authorship：Corresponding author   Language：English   Publisher：Frontiers in Molecular Neuroscience  

Synapses play a pivotal role in forming neural circuits, with critical implications for brain functions such as learning, memory, and emotions. Several advances in synaptic research have demonstrated the diversity of synaptic structure and function, which can form thousands of connections depending on the neuronal cell types. Moreover, synapses not only interconnect neurons but also establish connections with glial cells such as astrocytes, which play a key role in the architecture and function of neuronal circuits in the brain. Emerging evidence suggests that dysfunction of synaptic proteins contributes to a variety of neurological and psychiatric disorders. Therefore, it is crucial to determine the molecular networks within synapses in various neuronal cell types to gain a deeper understanding of how the nervous system regulates brain function. Recent advances in synaptic proteome approaches, such as fluorescence-activated synaptosome sorting (FASS) and proximity labeling, have allowed for a detailed and spatial analysis of many cell-type-specific synaptic molecules in vivo. In this brief review, we highlight these novel spatial proteomic approaches and discuss the regulation of synaptic formation and function in the brain. This knowledge of molecular networks provides new insight into the understanding of many neurological and psychiatric disorders.

DOI： 10.3389/fnmol.2024.1361956

Web of Science

Scopus

PubMed

Authorship：Last author,　Corresponding author  

DOI： https://doi.org/10.1016/j.neures.2023.01.005

Language：English