Updated on 2025/06/26

Information

 

写真a

 
OHTA KUNIMASA
 
Organization
Faculty of Arts and Science Division for Experimental Natural Science Professor
Graduate School of Systems Life Sciences Department of Systems Life Sciences（Concurrent）
Title
Professor
Contact information
メールアドレス
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0928026014
Profile
動物の形作りの基本となる幹細胞の増殖・分化制御機構を、マウスの脳神経幹細胞を対象に研究を行っている。我々が、単離・命名した分泌型タンパク質TsukushiとAkhirinは、脳神経幹細胞が局在する側脳室下垂と海馬に特異的に発現していることから、これら遺伝子のノックアウトマウスやトランスジェニックマウスを用いて、脳神経幹細胞ニッチ制御の分子メカニズムの解明を目指している。 また、我々は、生きた乳酸菌をヒト皮膚細胞に取り込ませると、細胞が多能性を獲得する現象を見出し（Ohta et al., 2012）、リボソームがこの形質転換を担うことを報告した（Ito et al., 2018）。真正細菌が古細菌に感染し、二つの生物が共生した後、真正細菌の遺伝子が移動し真核細胞が出現したことを唱える「細胞内共生説」を実験的に証明するユニークな研究も行っている。
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Research Areas

  • Life Science / Neuroscience-general

  • Life Science / Developmental biology

Degree

  • Ph.D. （ 1993.10 Kyushu University ）

Research History

  • Kyushu University Faculty od Arts and Science Professor 

    2020.4 - Present

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  • Kyushu University Faculty of Arts and Science Professor 

    2020.4 - Present

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    Country：Japan

  • 1992年4月ー1992年8月　日本学術振興事業団   

    1992年4月ー1992年8月　日本学術振興事業団

  • 1992年9月ー2019年3月　熊本大学   

Education

  • Kyushu University   理学部   生物学科

    1983.4 - 1987.3

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    Country：Japan

Research Interests・Research Keywords

  • Research theme： Regulation of neural stem cell by Akhrin

    Keyword： Akhirin

    Research period： 2020.4 - Present

  • Research theme： Transdifferentiation of cell by ribosome proteins

    Keyword： Ribosome

    Research period： 2020.4 - Present

  • Research theme： Regulation of neural stem cell by Tsukushi

    Keyword： Tsukushi

    Research period： 2020.4 - Present

  • Research theme： Cellular transdifferentiation by ribosome

    Keyword： Transdifferentiation, ribosome, stem cell

    Research period： 2010.4

  • Research theme： Regulation of neural stem cell niche by soluble molecules Tsukushi and Akhirin

    Keyword： neural stem cell, niche, Tsukushi, Akhirin

    Research period： 2010.4

Awards

  • 彩都賞

    2015.5   大阪府   乳酸菌を用いた繊維芽細胞を多能性細胞に転換する技術とそのリプログラミング因子

  • 熊本医学会奨励賞

    2005.5   熊本医学会   新規BMPアンタゴニストTsukushiによる眼の形態形成制御の解明

Papers

  • Dysfunction of the proteoglycan Tsukushi causes hydrocephalus through altered neurogenesis in the subventricular zone Reviewed International journal

    N. Ito, M. A. Riyadh, S. A. I. Ahmad, S. Hattori, Y. Kanemura, H. Kiyonari, T. Abe, Y. Furuta, Y. Shinmyo, N. Kaneko, Y. Hirota, G. Lupo, J. Hatakeyama, F. A. Abdulhaleem M, M. B. Anam, T. Yamaguchi, T. Takeo, H. Takebayashi, M. Takebayashi, Y. Oike, N. Nakagata, K. Shimamura, M. J. Holtzman, Y. Takahashi, F. Guillemot, K. Miyakawa, K. Sawamoto, and @K. Ohta

    Science Translational Medicine   13 ( 587 )   2021.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    The lateral ventricle (LV) is flanked by the subventricular zone (SVZ), a neural stem cell (NSC) niche rich in extrinsic growth factors regulating NSC maintenance, proliferation and neuronal differentiation. Dysregulation of the SVZ niche causes LV expansion, a condition known as hydrocephalus; however, the underlying pathological mechanisms areis unclear. We show that deficiency of the proteoglycan Tsukushi (TSK) in ependymal cells at the LV surface and in the cerebrospinal fluid (CSF) results in hydrocephalus with neurodevelopmental disorder-like symptoms in mice. These symptoms are accompanied by altered differentiation and survival of the NSC lineage, disrupted ependymal structure and dysregulated Wnt signaling. Multiple TSK variants found in patients with hydrocephalus patients exhibit reduced physiological activity in mice in vivo and in vitro. Administration of wild-type TSK protein or Wnt antagonists, but not of hydrocephalus-related TSK variants, in the LV of TSK knockout mice preventeds hydrocephalus and preserveds SVZ neurogenesis. These observations reveal suggest that TSK plays a crucial role for TSK as a niche molecule modulating the fate of SVZ NSCs and point to TSK as a candidate for the diagnosis and therapy of hydrocephalus.

    DOI： DOI: 10.1126/scitranslmed.aay7896

    Other Link： https://stm.sciencemag.org/content/13/587/eaay7896

  • Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency Reviewed

    Naofumi Ito, Kaoru Katoh, Hiroko Kushige, Yutaka Saito, Terumasa Umemoto, Yu Matsuzaki, Hiroshi Kiyonari, Daiki Kobayashi, Minami Soga, Takumi Era, Norie Araki, Yasuhide Furuta, Toshio Suda, Yasuyuki Kida, Kunimasa Ohta

    Scientific reports   8 ( 1 )   2018.12

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    Recently, we reported that bacterial incorporation induces cellular transdifferentiation of human fibroblasts. However, the bacterium-intrinsic cellular- transdifferentiation factor remained unknown. Here, we found that cellular transdifferentiation is caused by ribosomes. Ribosomes, isolated from both prokaryotic and eukaryotic cells, induce the formation of embryoid body-like cell clusters. Numerous ribosomes are incorporated into both the cytoplasm and nucleus through trypsin-activated endocytosis, which leads to cell-cluster formation. Although ribosome-induced cell clusters (RICs) express several stemness markers and differentiate into derivatives of all three germ layers in heterogeneous cell populations, RICs fail to proliferate, alter the methylation states of pluripotent genes, or contribute to teratoma or chimera formation. However, RICs express markers of epithelial–mesenchymal transition without altering the cell cycle, despite their proliferation obstruction. These findings demonstrate that incorporation of ribosomes into host cells induces cell transdifferentiation and alters cellular plasticity.

    DOI： 10.1038/s41598-018-20057-1

  • Lactic Acid Bacteria Convert Human Fibroblasts to Multipotent Cells Reviewed

    Kunimasa Ohta, Rie Kawano, Naofumi Ito

    PloS one   7 ( 12 )   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0051866

  • Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors Invited Reviewed International coauthorship

    Journal of Developmental Biology   2025.6

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/jdb13020019

  • Bacterial Ribosomes Induce Plasticity in Mouse Adult Fibroblasts. Reviewed

    Datta Anamika, Arif Istiaq, Shigehiko Tamura, Kunimasa Ohta

    Cells   13 ( 13 )   1116   2024.6

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/cells13131116.

  • Establishment and characterization of a novel cancer stem-like cell of cholangiocarcinoma. International journal

    Orasa Panawan, Atit Silsirivanit, Chih-Hsiang Chang, Siyaporn Putthisen, Piyanard Boonnate, Taro Yokota, Yuki Nishisyama-Ikeda, Marutpong Detarya, Kanlayanee Sawanyawisuth, Worasak Kaewkong, Kanha Muisuk, Sukanya Luang, Kulthida Vaeteewoottacharn, Ryosho Kariya, Hiromu Yano, Yoshihiro Komohara, Kunimasa Ohta, Seiji Okada, Sopit Wongkham, Norie Araki

    Cancer science   114 ( 8 )   3230 - 3246   2023.8   （ ISSN:1347-9032 eISSN:1349-7006 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cancer Science  

    Cholangiocarcinoma (CCA) is an aggressive malignant tumor of bile duct epithelia. Recent evidence suggests the impact of cancer stem cells (CSC) on the therapeutic resistance of CCA; however, the knowledge of CSC in CCA is limited due to the lack of a CSC model. In this study, we successfully established a stable sphere-forming CCA stem-like cell, KKU-055-CSC, from the original CCA cell line, KKU-055. The KKU-055-CSC exhibits CSC characteristics, including 1) the ability to grow stably and withstand continuous passage for a long period of culture in the stem cell medium, 2) high expression of stem cell markers, 3) low responsiveness to standard chemo-drugs, 4) multi-lineage differentiation, and 5) faster and constant expansive tumor formation in xenograft mouse models. To identify the CCA-CSC-associated pathway, we have performed a global proteomics and functional cluster/network analysis. Proteomics identified the 5925 proteins in total, and the significantly upregulated proteins in CSC compared with FCS-induced differentiated CSC and its parental cells were extracted. Network analysis revealed that high mobility group A1 (HMGA1) and Aurora-A signaling via the STAT-3 pathways were enriched in KKU-055-CSC. Knockdown of HMGA1 in KKU-055-CSC suppressed the expression of stem cell markers, induced the differentiation followed by cell proliferation, and enhanced sensitivity to chemo drugs including Aurora A inhibitors. In silico analysis demonstrated that the expression of HMGA1 was correlated with Aurora-A expressions and poor survival of CCA patients. In conclusion, we have established a unique CCA stem-like cell model and identified the HMGA1-Aurora-A signaling as an important pathway for CSC-CCA.

    DOI： 10.1111/cas.15812

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  • 胆管癌の新規癌幹細胞様細胞の樹立と特性評価(Establishment and characterization of a novel cancer stem-like cell of cholangiocarcinoma)

    Panawan Orasa, Silsirivanit Atit, Chang Chih-Hsiang, Putthisen Siyaporn, Boonnate Piyanard, Yokota Taro, Nishisyama-Ikeda Yuki, Detarya Marutpong, Sawanyawisuth Kanlayanee, Kaewkong Worasak, Muisuk Kanha, Luang Sukanya, Vaeteewoottacharn Kulthida, Kariya Ryusho, Yano Hiromu, Komohara Yoshihiro, Ohta Kunimasa, Okada Seiji, Wongkham Sopit, Araki Norie

    Cancer Science   114 ( 8 )   3230 - 3246   2023.8   （ ISSN:1347-9032 ）

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    Language：English   Publisher：John Wiley & Sons Australia, Ltd  

    56歳のタイ人男性の低分化胆管癌組織に由来する胆管癌細胞株KKU-055から癌幹細胞(CSC)様のKKU-055-CSCを樹立した。KKU-055-CSCは、(1)幹細胞培地中で安定に増殖し、長期間の連続継代培養に耐える能力、(2)幹細胞マーカーの高発現、(3)標準的な化学療法薬に対する低反応性、(4)多系統分化、(5)異種移植マウスモデルにおける迅速かつ一定の腫瘍形成、などのCSC特性を示した。プロテオミクス解析で合計5925個のタンパク質を同定し、ウシ胎児血清で分化誘導したKKU-055-CSCおよびKKU-055-CSCと比較して、KKU-055-CSCで有意に発現上昇したタンパク質を抽出した。ネットワーク解析の結果、KKU-055-CSCでは、高移動度グループA1(HMGA1)とシグナル伝達および転写活性化因子3経路を介したオーロラAシグナル伝達が濃縮されていた。KKU-055-CSCにおけるHMGA1のノックダウンは、幹細胞マーカーの発現を抑制し、細胞分化と増殖を誘導し、オーロラA阻害剤を含む化学療法剤に対する感受性を増強した。in silico解析の結果、HMGA1の発現はオーロラAの発現およびCCA患者の生存率低下と相関していることが示された。以上より、胆管癌幹様細胞モデルを確立し、HMGA1-オーロラAシグナルがCSC様胆管癌にとって重要な経路であることが明らかとなった。

  • A review on Tsukushi: mammalian development, disorders, and therapy. Reviewed International journal

    Arif Istiaq, Kunimasa Ohta

    Journal of cell communication and signaling   16 ( 4 )   505 - 513   2022.12   （ ISSN:1873-9601 eISSN:1873-961X ）

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Cell Communication and Signaling  

    Tsukushi (TSK), a leucine-rich peptidoglycan in the extracellular compartment, mediates multiple signaling pathways that are critical for development and metabolism. TSK regulates signaling pathways that eventually control cellular communication, proliferation, and cell fate determination. Research on TSK has become more sophisticated in recent years, illustrating its involvement in the physiology and pathophysiology of neural, genetic, and metabolic diseases. In a recent study, we showed that TSK therapy reversed the pathophysiological abnormalities of the hydrocephalic (a neurological disorder) brain in mice. This review summarizes the roles of TSK in key signaling processes in the mammalian development, disorders, and evaluating its possible therapeutic and diagnostic potential.

    DOI： 10.1007/s12079-022-00669-z

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  • Tsukushi proteoglycan maintains RNA splicing and developmental signaling network in GFAP-expressing subventricular zone neural stem/progenitor cells Reviewed International journal

    @Arif Istiaq, Terumasa Umemoto, Naofumi Ito, Toshio Suda, Kenji Shimamura, and @Kunimasa Ohta

    Frontiers in Cell and Developmental Biology   10   994588 - 994588   2022.11   （ ISSN:2296-634X eISSN:2296-634X ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Tsukushi (TSK) proteoglycan dysfunction leads to hydrocephalus, a condition defined by excessive fluid collection in the ventricles and lateral ventricular enlargement. TSK injections into the LV at birth are effective at rescuing the lateral ventricle (LV). TSK regulates the activation of the Wnt signaling to facilitate the proper expansion of the LV and maintain the fate of the neural stem cell lineage. However, the molecular mechanism by which TSK acts on neural stem/progenitor cells (NSCs) during LV development is unknown. We demonstrated that TSK is crucial for the splicing and development-associated gene regulation of GFAP-expressing subventricular zone (SVZ) NSCs. We isolated GFAP-expressing NSCs from the SVZ of wild-type (GFAP<sup>GFP/+</sup>/TSK<sup>+/+</sup>) and TSK knock-out (GFAP<sup>GFP/+</sup>/TSK<sup>−/−</sup>) mice on postnatal day 3 and compared their transcriptome and splicing profiles. TSK deficiency in NSCs resulted in genome-wide missplicing (alteration in exon usage) and transcriptional dysregulation affecting the post-transcriptional regulatory processes (including splicing, cell cycle, and circadian rhythm) and developmental signaling networks specific to the cell (including Wnt, Sonic Hedgehog, and mTOR signaling). Furthermore, TSK deficiency prominently affected the splicing of genes encoding RNA and DNA binding proteins in the nervous SVZ and non-nervous muscle tissues. These results suggested that TSK is involved in the maintenance of correct splicing and gene regulation in GFAP-expressing NSCs, thereby protecting cell fate and LV development. Hence, our study provides a critical insight on hydrocephalus development.

    File： 2022-FCDB-Arif.pdf

    DOI： 10.3389/fcell.2022.994588

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    Other Link： https://www.frontiersin.org/articles/10.3389/fcell.2022.994588/full

  • Assessing the Role of Ependymal and Vascular Cells as Sources of Extracellular Cues Regulating the Mouse Ventricular-Subventricular Zone Neurogenic Niche. Reviewed International journal

    Sabrina Quaresima, Arif Istiaq, Hirofumi Jono, Emanuele Cacci, Kunimasa Ohta, Giuseppe Lupo

    Frontiers in cell and developmental biology   10   845567 - 845567   2022.4   （ ISSN:2296-634X ）

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers in Cell and Developmental Biology  

    Neurogenesis persists in selected regions of the adult mouse brain; among them, the ventricular-subventricular zone (V-SVZ) of the lateral ventricles represents a major experimental paradigm due to its conspicuous neurogenic output. Postnatal V-SVZ neurogenesis is maintained by a resident population of neural stem cells (NSCs). Although V-SVZ NSCs are largely quiescent, they can be activated to enter the cell cycle, self-renew and generate progeny that gives rise to olfactory bulb interneurons. These adult-born neurons integrate into existing circuits to modify cognitive functions in response to external stimuli, but cells shed by V-SVZ NSCs can also reach injured brain regions, suggesting a latent regenerative potential. The V-SVZ is endowed with a specialized microenvironment, which is essential to maintain the proliferative and neurogenic potential of NSCs, and to preserve the NSC pool from exhaustion by finely tuning their quiescent and active states. Intercellular communication is paramount to the stem cell niche properties of the V-SVZ, and several extracellular signals acting in the niche milieu have been identified. An important part of these signals comes from non-neural cell types, such as local vascular cells, ependymal and glial cells. Understanding the crosstalk between NSCs and other niche components may aid therapeutic approaches for neuropathological conditions, since neurodevelopmental disorders, age-related cognitive decline and neurodegenerative diseases have been associated with dysfunctional neurogenic niches. Here, we review recent advances in the study of the complex interactions between V-SVZ NSCs and their cellular niche. We focus on the extracellular cues produced by ependymal and vascular cells that regulate NSC behavior in the mouse postnatal V-SVZ, and discuss the potential implication of these molecular signals in pathological conditions.

    DOI： 10.3389/fcell.2022.845567

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  • Ribosome Incorporation Induces EMT-like Phenomenon with Cell Cycle Arrest in Human Breast Cancer Cell

    Kudo, M; Anam, MB; Istiaq, A; Ahmad, SAI; Ito, N; Ohta, K

    CELLS TISSUES ORGANS   211 ( 2 )   212 - 221   2022.3   （ ISSN:1422-6405 eISSN:1422-6421 ）

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    Language：English   Publisher：Cells Tissues Organs  

    Although ribosomes are generally known to be a translational machinery, some ribosomal proteins also have accessory functions involving early development and differentiation. Previously, we reported that ribosome incorporation into human dermal fibroblasts generated embryoid body-like cell clusters, altered cellular fate, and differentiated into cells of all 3 germ layers. However, the molecular phenomena induced by ribosome incorporation in the cell remained unknown. Here, we demonstrate that ribosome incorporation into human breast cancer cell MCF7 leads to ribosome-induced cell clusters (RICs) formation accompanying with epithelial-mesenchymal transition (EMT)-like gene expression. Following ribosome incorporation, MCF7 cells cease proliferation, which is caused by inhibition of cell cycle transition from G0 to G1 phase. Further, MCF7 RICs show induced expression of EMT markers, TGF-β1 and Snail along with autophagy markers and tumor suppressor gene p53. These findings indicate that the incorporation of ribosome into cancer cells induces an EMT-like phenomenon and changes the cancer cell characteristics.

    DOI： 10.1159/000513908

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  • TOPICS 細胞生物学 プロテオグリカンTsukushiによる脳神経幹細胞ニッチの制御

    太田 訓正

    医学のあゆみ   280 ( 3 )   234 - 235   2022.1   （ ISSN:00392359 ）

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    Publisher：医歯薬出版  

    DOI： 10.32118/ayu28003234

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  • Targeting alpha 2,3-sialylated glycan in glioma stem-like cells by Maackia amurensis lectin-II: A promising strategy for glioma treatment Reviewed International journal

    Siyaporn Putthisen, Atit Silsirivanit, Orasa Panawan, Akiko Niibori-Nambu, Yuki Nishiyama-Ikeda, Prasertsri Ma-In, Sukanya Luang, Kunimasa Ohta, Kanha Muisuk, Sopit Wongkham, Norie Araki

    Experimental Cell Research   410 ( 1 )   112949 - 112949   2022.1   （ ISSN:0014-4827 eISSN:1090-2422 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    Glioma stem/initiating cells have been considered a major cause of tumor recurrence and therapeutic resistance. In this study, we have established a new glioma stem-like cell (GSC), named U373-GSC, from the U373 glioma cell line. The cells exhibited stemness properties, e.g., expression of stem cell markers, self-renewal activity, multi-lineage differentiating abilities, and drug resistance. Using U373-GSC and GSC-03A–a GSC clone previously established from patient tissue, we have identified a novel GSC-associated sialic acid-modified glycan commonly expressed in both cell lines. Lectin fluorescence staining showed that Maackia amurensis lectin II (MAL–II)–binding alpha2,3-sialylated glycan (MAL-SG) was highly expressed in GSCs, and drastically decreased during FBS induced differentiation to glioma cells or little in the parental cells. Treatment of GSCs by MAL-II, compared with other lectins, showed that MAL-II significantly suppresses cell viability and sphere formation via induction of cell cycle arrest and apoptosis of the GSCs. Similar effects were observed when the cells were treated with a sialyltransferase inhibitor or sialidase. Taken together, we demonstrate for the first time that MAL-SGs/alpha-2,3 sialylations are upregulated and control survival/maintenances of GSCs, and their functional inhibitions lead to apoptosis of GSCs. MAL-SG could be a potential marker and therapeutic target of GSCs; its inhibitors, such as MAL-II, may be useful for glioma treatment in the future.

    DOI： 10.1016/j.yexcr.2021.112949

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  • Extrinsic ribosome stimuli triggers development of glioblastoma stem like cells

    Jono Hirofumi, Shirakawa Yuki, Miyake Shunsuke, Kanemaru Ayumi, Yonemaru Kou, Uchino Shota, Hide Takuichiro, Mukasa Akitake, Ohta Kunimasa, Saito Hideyuki

    Proceedings for Annual Meeting of The Japanese Pharmacological Society   96 ( 0 )   4-B-O12-2   2022   （ eISSN:24354953 ）

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    Language：Japanese   Publisher：Japanese Pharmacological Society  

    <p><b><u>Background</u></b>: Although glioblastoma (GBM) stem like cells (GSCs), which exhibit chemo-radio resistance and recurrence, are key prognostic factors in GBM patients, molecular mechanisms of GSCs development are largely unknown. Recent studies revealed that extrinsic ribosome incorporation into somatic cells played key roles in cell reprogramming process towards stem cell properties. In this study, we sought to elucidate the mechanisms underlying GSCs development by focusing extrinsic ribosome incorporation into GBM cells. </p><p><b><u>Results</u></b>: The ribosome incorporation into GBM cells significantly increased ribosome induced cancer cell spheroid (RICCS) formation, and showed the stem like cell characters. In RICCS, phosphorylation and protein expression of RPS6, an intrinsic ribosomal protein, and STAT3 phosphorylation were involved in regulating cell spheroids formation. Interestingly, glioma-derived extrinsic ribosome also promoted GBM-RICCS formation through the intrinsic RPS6 phosphorylation. Moreover, in glioma patients, RPS6 phosphorylation was observed in higher grade glioma tissues, and predominantly up-regulated in GSCs niches, such as perinecrosis niche and perivascular niche. </p><p><b><u>Conclusion</u></b>: Our results suggest the potential biological & clinical significance of extrinsic ribosomal proteins in GSCs development.</p>

    DOI： 10.1254/jpssuppl.96.0_4-b-o12-2

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  • Glioma Cells Acquire Stem-like Characters by Extrinsic Ribosome Stimuli Invited Reviewed International journal

    Yuki Shirakawa, @Kunimasa Ohta, Shunsuke Miyake, Ayumi Kanemaru, Akari Kuwano, Kou Yonemaru, Shota Uchino, Michiko Yamaoka, Yuki Ito, Naofumi Ito, Takuichiro Hide, Naoki Shinojima, Akitake Mukasa, Hideyuki Saito, and Hirofumi Jono

    Cells   10 ( 11 )   2970   2021.11

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    Other Link： https://doi.org/10.3390/cells10112970

  • Tsukushi is essential for the formation of the posterior semicircular canal that detects gait performance Invited Reviewed International journal

    Miwa, T., Ito, N., and @Ohta, K.

    J Cell Commun Signal.   2021.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12079-021-00627-1

    Other Link： https://pubmed.ncbi.nlm.nih.gov/34061311/

  • Ribosome induces transdifferentiation of A549 and H-111-TC cancer cell lines Reviewed International journal

    Mohammad Badrul Anam, Arif Istiaq, Ryusho Kariya, #Mikiko Kudo, Shah Adil Ishtiyaq Ahmad, Naofumi Ito, Seiji Okada, and @Kunimasa Ohta

    Biochemistry and Biophysics Reports   2021.2

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    DOI： https://doi.org/10.1016/j.bbrep.2021.100946

  • Induces EMT-like Phenomenon with Cell Cycle Arrest in Human Breast Cancer Cell Invited Reviewed International journal

    #Kudo M., Anam M.B., Istiaq A., Ahmad S.A.I., Ito N., and @Ohta K.

    Cells Tissues Organs   2021.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： doi.org/10.1159/000513908

  • Ribosomal protein S6 promotes stem-like characters in glioma cells. Reviewed International journal

    Yuki Shirakawa, Takuichiro Hide, Michiko Yamaoka, Yuki Ito, Naofumi Ito,@ Kunimasa Ohta, Naoki Shinojima, Akitake Mukasa, Hideyuki Saito, Hirofumi Jono

    Cancer science   111 ( 6 )   2041 - 2051   2020.6

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    Glioblastoma multiforme (GBM), a lethal brain tumor developing in the white matter of the adult brain, contains a small population of GBM stem cells (GSCs), which potentially cause chemotherapeutic resistance and tumor recurrence. However, the mechanisms underlying the pathogenesis and maintenance of GSCs remain largely unknown. A recent study reported that incorporation of ribosomes and ribosomal proteins into somatic cells promoted lineage trans-differentiation toward multipotency. This study aimed to investigate the mechanism underlying stemness acquisition in GBM cells by focusing on 40S ribosomal protein S6 (RPS6). RPS6 was significantly upregulated in high-grade glioma and localized at perivascular, perinecrotic, and border niches in GBM tissues. siRNA-mediated RPS6 knock-down significantly suppressed the characteristics of GSCs, including their tumorsphere potential and GSC marker expression; STAT3 was downregulated in GBM cells. RPS6 overexpression enhanced the tumorsphere potential of GSCs and these effects were attenuated by STAT3 inhibitor (AG490). Moreover, RPS6 expression was significantly correlated with SOX2 expression in different glioma grades. Immunohistochemistry data herein indicated that RPS6 was predominant in GSC niches, concurrent with the data from IVY GAP databases. Furthermore, RPS6 and other ribosomal proteins were upregulated in GSC-predominant areas in this database. The present results indicate that, in GSC niches, ribosomal proteins play crucial roles in the development and maintenance of GSCs and are clinically associated with chemoradioresistance and GBM recurrence.

    DOI： 10.1111/cas.14399

  • Tsukushi is essential for the development of the inner ear Reviewed

    Toru Miwa, Kunimasa @Ohta, Naofumi Ito, Satoko Hattori, Tsuyoshi Miyakawa, Toru Takeo, Naomi Nakagata, Wen Jie Song, Ryosei Minoda

    Molecular Brain   13 ( 1 )   2020.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s13041-020-00570-z

  • Akhirin regulates the proliferation and differentiation of neural stem cells/progenitor cells at neurogenic niches in mouse brain Reviewed

    Mohammad Badrul Anam, Shah Adil Ishtiyaq Ahmad, #Mikiko Kudo, Arif Istiaq, Athary Abdulhaleem M. Felemban, Naofumi Ito, @Kunimasa Ohta

    Development Growth and Differentiation   62 ( 2 )   97 - 107   2020.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/dgd.12646

  • Tsukushi is essential for proper maintenance and terminal differentiation of mouse hippocampal neural stem cells Reviewed

    Shah Adil Ishtiyaq Ahmad, Mohammad Badrul Anam, Arif Istiaq, Naofumi Ito, @Kunimasa Ohta

    Development Growth and Differentiation   62 ( 2 )   108 - 117   2020.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/dgd.12649

  • Significance of Tsukushi in lung cancer Reviewed

    Tatsuya Yamada, Kunimasa Ohta, Yamato Motooka, Kosuke Fujino, Shinji Kudoh, Yuki Tenjin, Younosuke Sato, Akira Matsuo, Koei Ikeda, Makoto Suzuki, Takaaki Ito

    Lung Cancer   131   104 - 111   2019.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.lungcan.2019.03.024

  • CCN2/CTGF binds the small leucine rich proteoglycan protein Tsukushi Reviewed

    Kunimasa Ohta, Eriko Aoyama, Shah Adil Ishtiyaq Ahmad, Naofumi Ito, Mohammad Badrul Anam, Satoshi Kubota, Masaharu Takigawa

    Journal of Cell Communication and Signaling   13 ( 1 )   113 - 118   2019.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12079-018-0487-x

  • The hepatokine Tsukushi gates energy expenditure via brown fat sympathetic innervation Reviewed

    Qiuyu Wang, Vishal P. Sharma, Hong Shen, Yuanyuan Xiao, Qi Zhu, Xuelian Xiong, Liang Guo, Lin Jiang, Kunimasa Ohta, Siming Li, Haifei Shi, Liangyou Rui, Jiandie D. Lin

    Nature Metabolism   1 ( 2 )   251 - 260   2019.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42255-018-0020-9

  • Sirtuin 7 is involved in the consolidation of fear memory in mice Reviewed

    Md Safiqul Islam, Fan Yan Wei, Kunimasa Ohta, Naoki Shigematsu, Takaichi Fukuda, Kazuhito Tomizawa, Tatsuya Yoshizawa, Kazuya Yamagata

    Biochemical and Biophysical Research Communications   495 ( 1 )   261 - 266   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.10.159

  • The role of Tsukushi (TSK), a small leucine-rich repeat proteoglycan, in bone growth Reviewed

    Kosei Yano, Kaoru Washio, Yuka Tsumanuma, Masayuki Yamato, Kunimasa Ohta, Teruo Okano, Yuichi Izumi

    Regenerative Therapy   7   98 - 107   2017.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.reth.2017.08.001

  • Cadherin-7 enhances Sonic Hedgehog signalling by preventing Gli3 repressor formation during neural tube patterning Reviewed

    Rie Kawano, Kunimasa Ohta, Giuseppe Lupo

    Open Biology   7 ( 12 )   2017.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1098/rsob.170225

  • Tsukushi expression is dependent on Notch signaling and oscillated in the presomitic mesoderm during chick somitogenesis Reviewed

    Uzzal Kumar Acharjee, Ryu Gejima, M. Felemban Athary Abdulhaleem, M. Asrafuzzaman Riyadh, Hideaki Tanaka, Kunimasa Ohta

    Biochemical and Biophysical Research Communications   465 ( 3 )   625 - 630   2015.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2015.08.074

  • Akhirin regulates the proliferation and differentiation of neural stem cells in intact and injured mouse spinal cord Reviewed

    Felemban Athary Abdulhaleem M, Xiaohong Song, Rie Kawano, Naohiro Uezono, Ayako Ito, Giasuddin Ahmed, Mahmud Hossain, Kinichi Nakashima, Hideaki Tanaka, Kunimasa Ohta

    Developmental Neurobiology   75 ( 5 )   494 - 504   2015.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/dneu.22238

  • Draxin from neocortical neurons controls the guidance of thalamocortical projections into the neocortex Reviewed

    Yohei Shinmyo, M. Asrafuzzaman Riyadh, Giasuddin Ahmed, Iftekhar Bin Naser, Mahmud Hossain, Hirohide Takebayashi, Hiroshi Kawasaki, Kunimasa Ohta, Hideaki Tanaka

    Nature communications   6   2015.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncomms10232

  • Tsukushi is involved in the wound healing by regulating the expression of cytokines and growth factors Reviewed

    Daisuke Niimori, Rie Kawano, Kanako Niimori-Kita, Hironobu Ihn, Kunimasa Ohta

    Journal of Cell Communication and Signaling   8 ( 3 )   173 - 177   2014.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12079-014-0241-y

  • Inhibitory effects of draxin on axonal outgrowth and migration of precerebellar neurons Reviewed

    M. Asrafuzzaman Riyadh, Yohei Shinmyo, Kunimasa Ohta, Hideaki Tanaka

    Biochemical and Biophysical Research Communications   449 ( 1 )   169 - 174   2014.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2014.05.013

  • Equarin Is Involved in Cell Adhesion by Means of Heparan Sulfate Proteoglycan During Lens Development Reviewed

    Xiaohong Song, Yuya Sato, Kiyotoshi Sekiguchi, Hideaki Tanaka, Kunimasa Ohta

    Developmental Dynamics   242 ( 3 )   301   2013.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/dvdy.23921

  • Amygdala kindling induces nestin expression in the leptomeninges of the neocortex Reviewed

    Shogo Ninomiya, Shigeyuki Esumi, Kunimasa Ohta, Takaichi Fukuda, Tetsufumi Ito, Itaru Imayoshi, Ryoichiro Kageyama, Toshio Ikeda, Shigeyoshi Itohara, Nobuaki Tamamaki

    Neuroscience Research   75 ( 2 )   121 - 129   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.neures.2012.12.006

  • The combinatorial guidance activities of draxin and Tsukushi are essential for forebrain commissure formation Reviewed

    Mahmud Hossain, Giasuddin Ahmed, Iftekhar Bin Naser, Yohei Shinmyo, Ayako Ito, M. Asrafuzzaman Riyadh, Athary Felemban, Xiaohong Song, Kunimasa Ohta, Hideaki Tanaka

    Developmental Biology   374 ( 1 )   58 - 70   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ydbio.2012.11.029

  • Equarin is involved in cell adhesion by means of heparan sulfate proteoglycan during lens development Reviewed

    Xiaohong Song, Yuya Sato, Kiyotoshi Sekiguchi, Hideaki Tanaka, Kunimasa Ohta

    Developmental Dynamics   242 ( 1 )   23 - 29   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/dvdy.23902

  • Tsukushi controls the hair cycle by regulating TGF-Β1 signaling Reviewed

    Daisuke Niimori, Rie Kawano, Athary Felemban, Kanako Niimori-Kita, Hideaki Tanaka, Hironobu Ihn, Kunimasa Ohta

    Developmental Biology   372 ( 1 )   81 - 87   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ydbio.2012.08.030

  • Equarin is involved as an FGF signaling modulator in chick lens differentiation Reviewed

    Xiaohong Song, Yuya Sato, Athary Felemban, Ayako Ito, Mahmud Hossain, Hiroshi Ochiai, Takashi Yamamoto, Kiyotoshi Sekiguchi, Hideaki Tanaka, Kunimasa Ohta

    Developmental Biology   368 ( 1 )   109 - 117   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ydbio.2012.05.029

  • Draxin inhibits axonal outgrowth through the netrin receptor dcc Reviewed

    Giasuddin Ahmed, Yohei Shinmyo, Kunimasa Ohta, Shahidul M. Islam, Mahmud Hossain, Iftekhar Bin Naser, M. Asrafuzzaman Riyadh, Yuhong Su, Sanbing Zhang, Marc Tessier-Lavigne, Hideaki Tanaka

    Journal of Neuroscience   31 ( 39 )   14018 - 14023   2011.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1523/JNEUROSCI.0943-11.2011

  • Tsukushi functions as a Wnt signaling inhibitor by competing with Wnt2b for binding to transmembrane protein Frizzled4 Reviewed

    Kunimasa Ohta, Ayako Ito, Sei Kuriyama, Giuseppe Lupo, Mitsuko Kosaka, Shin Ichi Ohnuma, Shinichi Nakagawa, Hideaki Tanaka

    Proceedings of the National Academy of Sciences of the United States of America   108 ( 36 )   14962 - 14967   2011.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1100513108

  • Tsukushi is required for anterior commissure formation in mouse brain Reviewed

    Ayako Ito, Yohei Shinmyo, Takaya Abe, Naoko Oshima, Hideaki Tanaka, Kunimasa Ohta

    Biochemical and Biophysical Research Communications   402 ( 4 )   813 - 818   2010.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2010.10.127

  • PC3 is involved in the shift from proliferation to differentiation and maturation in spiral ganglion neurons Reviewed

    Momoko Hayashida, Ryosei Minoda, Yohei Shinmyo, Kunimasa Ohta, Hideaki Tanaka, Eiji Yumoto

    NeuroReport   21 ( 2 )   90 - 93   2010.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1097/WNR.0b013e328332c4d7

  • Anterior shift in gene expression precedes anteriormost digit formation in amniote limbs Reviewed

    Asaka Uejima, Takanori Amano, Naoki Nomura, Miyuki Noro, Taiji Yasue, Toshihiko Shiroishi, Kunimasa Ohta, Hitoshi Yokoyama, Koji Tamura

    Development Growth and Differentiation   52 ( 2 )   223 - 234   2010.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1440-169X.2009.01161.x

  • Draxin, a repulsive guidance protein for spinal cord and forebrain commissures Reviewed

    Shahidul M. Islam, Yohei Shinmyo, Tatsuya Okafuji, Yuhong Su, Iftekhar Bin Naser, Giasuddin Ahmed, Sanbing Zhang, Sandy Chen, Kunimasa Ohta, Hiroshi Kiyonari, Takaya Abe, Satomi Tanaka, Ryuichi Nishinakamura, Toshio Terashima, Toshio Kitamura, Hideaki Tanaka

    Science   323 ( 5912 )   388 - 393   2009.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1165187

  • Tsukushi modulates Xnr2, FGF and BMP signaling Regulation of Xenopus germ layer formation Reviewed

    Samantha A. Morris, Alexandra D. Almeida, Hideaki Tanaka, Kunimasa Ohta, Shin Ichi Ohnuma

    PloS one   2 ( 10 )   2007.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0001004

  • Tsukushi cooperates with VG1 to induce primitive streak and Hensen's node formation in the chick embryo Reviewed

    Kunimasa Ohta, Sei Kuriyama, Tatsuya Okafuji, Ryu Gejima, Shin Ichi Ohnuma, Hideaki Tanaka

    Development   133 ( 19 )   3777 - 3786   2006.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.02579

  • Tsukushi controls ectodermal patterning and neural crest specification in Xenopus by direct regulation of BMP4 and X-delta-1 activity Reviewed

    Sei Kuriyama, Giuseppe Lupo, Kunimasa Ohta, Shin Ichi Ohnuma, William A. Harris, Hideaki Tanaka

    Development   133 ( 1 )   75 - 88   2006.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.02178

  • Novel soluble molecule, Akhirin, is expressed in the embryonic chick eyes and exhibits heterophilic cell-adhesion activity Reviewed

    Mansoor Ahsan, Kunimasa Ohta, Sei Kuriyama, Hideaki Tanaka

    Developmental Dynamics   233 ( 1 )   95 - 104   2005.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/dvdy.20303

  • Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin Reviewed

    Kunimasa Ohta, Giuseppe Lupo, Sei Kuriyama, Roger Keynes, Christine E. Holt, William A. Harris, Hideaki Tanaka, Shin Ichi Ohnuma

    Developmental Cell   7 ( 3 )   347 - 358   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.devcel.2004.08.014

  • Molecular analysis of axon repulsion by the notochord Reviewed

    Christopher N.G. Anderson, Kunimasa Ohta, Marie M. Quick, Angeleen Fleming, Roger Keynes, David Tannahill

    Development   130 ( 6 )   1123 - 1133   2003.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.00327

  • Equarin, a novel soluble molecule expressed with polarity at chick embryonic lens equator, is involved in eye formation Reviewed

    Hong Mu, Kunimasa Ohta, Sei Kuriyama, Naoko Shimada, Hidenobu Tanihara, Kunio Yasuda, Hideaki Tanaka

    Mechanisms of Development   120 ( 2 )   143 - 155   2003.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0925-4773(02)00423-9

  • Analysis of ephrin-A2 in the chick retinotectal projection using a function-blocking monoclonal antibody Reviewed

    T. Yamada, T. Okafuji, K. Ohta, C. Handwerker, U. Drescher, H. Tanaka

    Journal of Neurobiology   47 ( 4 )   245 - 254   2001.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/neu.1032

  • Expression of Eph receptor tyrosine kinases and their ligands in chick embryonic motor neurons and hindlimb muscles Reviewed

    Hiroko Iwamasa, Kunimasa Ohta, Tomoko Yamada, Kazuo Ushijima, Hidenori Terasaki, Hideaki Tanaka

    Development Growth and Differentiation   41 ( 6 )   685 - 698   1999.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1046/j.1440-169X.1999.00468.x

  • Embryonic lens repels retinal ganglion cell axons Reviewed

    Kunimasa Ohta, David Tannahill, Kanako Yoshida, Alan R. Johnson, Geoffrey M.W. Cook, Roger J. Keynes

    Developmental Biology   211 ( 1 )   124 - 132   1999.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1006/dbio.1999.9312

  • Cloning and functional expression of an SGLT-1-like protein from the Xenopus laevis intestine Reviewed

    Katsumi Nagata, Naohiro Hori, Kenzo Sato, Kunimasa Ohta, Hideaki Tanaka, Yasutake Hiji

    American Journal of Physiology - Gastrointestinal and Liver Physiology   276 ( 5 39-5 )   G1251 - G1259   1999.5

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Normal ontogenic observations on the expression of Eph receptor tyrosine kinase, Cek8, in chick embryos Reviewed

    Shigeki Hirano, Hideaki Tanaka, Kunimasa Ohta, Masao Norita, Kaeko Hoshino, Reiko Meguro, Masahiko Kase

    Anatomy and Embryology   197 ( 3 )   187 - 197   1998.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s004290050130

  • The inhibitory effect on neurite outgrowth of motoneurons exerted by the ligands ELF-1 and RAGS Reviewed

    K Ohta, H Iwamasa, U Drescher, H Terasaki, H Tanaka

    MECHANISMS OF DEVELOPMENT   64 ( 1-2 )   127 - 135   1997.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    Eph-related receptor tyrosine kinases and ligands are expressed at high levels in the developing nervous system, giving rise to the proposal that they are involved in neuronal connection. Cek8 was found to be predominantly expressed on a subset of motoneurons innervating limb but not body muscles during motoneuron axonal growth. Here we show that the ligands RAGS and ELF-1 were expressed in limb buds and that they activated Cek8 when presented in membrane-bound or clustered forms of Fe chimeric proteins but not in unclustered soluble forms. When chick embryonic motoneurons enriched by panning were cultured on clustered farms of RAGS-Fc and ELF-I-Fc, the neurite growth of motoneurons expressing Cek8 was inhibited. Our results show a relationship between receptor phosphorylation and neurite growth inhibition and suggest that Eph-related kinases and Ligands have a regulatory effect on the axon growth of motoneurons during development. (C) 1997 Elsevier Science Ireland Ltd.

    DOI： 10.1016/S0925-4773(97)00056-7

  • Shared and distinct functions of RAGS and ELF-1 in guiding retinal axons Reviewed

    Bruno Monschau, Claus Kremoser, Kunimasa Ohta, Hideaki Tanaka, Tomomi Kaneko, Tomoko Yamada, Claudia Handwerker, Martin R. Hornberger, Jürgen Löschinger, Elena B. Pasquale, Doyle A. Siever, Michael F. Verderame, Bernhard K. Müller, Friedrich Bonhoeffer, Uwe Drescher

    EMBO Journal   16 ( 6 )   1258 - 1267   1997.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/emboj/16.6.1258

  • Developmental and denervation changes in c-ret proto-oncogene expression in chick motoneurons Reviewed

    Masaaki Nakamura, Kunimasa Ohta, Kaoru Hirokawa, Mikiko Fukushima, Makoto Uchino, Masayuki Ando, Hideaki Tanaka

    Molecular Brain Research   39 ( 1-2 )   1 - 11   1996.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0169-328X(95)00347-U

  • Regional specification of motoneurons along the anterior-posterior axis is independent of the notochord Reviewed

    Mikiko Fukushima, Masaaki Nakamura, Kunimasa Ohta, Ryouichi Okamura, Akira Negi, Hideaki Tanaka

    Development   122 ( 3 )   905 - 914   1996.3

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    Language：English   Publishing type：Research paper (scientific journal)  

  • The receptor tyrosine kinase, Cek8, is transiently expressed on subtypes of motoneurons in the spinal cord during development Reviewed

    Kunimasa Ohta, Masaaki Nakamura, Kaoru Hirokawa, Shigeyasu Tanaka, Atsushi Iwama, Toshio Suda, Masayuki Ando, Hideaki Tanaka

    Mechanisms of Development   54 ( 1 )   59 - 69   1996.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0925-4773(95)00461-0

  • Plexin A novel neuronal cell surface molecule that mediates cell adhesion via a homophilic binding mechanism in the presence of calcium ions Reviewed

    Kunimasa Ohta, Akihito Mizutani, Atsushi Kawakami, Yasunori Murakami, Yasuyo Kasuya, Shin Takagi, Hideaki Tanaka, Hajime Fujisawa

    Neuron   14 ( 6 )   1189 - 1199   1995.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0896-6273(95)90266-X

  • Differential expression of two cell surface proteins, neuropilin and plexin, in Xenopus olfactory axon subclasses Reviewed

    M. Satoda, S. Takagi, K. Ohta, T. Hirata, H. Fujisawa

    Journal of Neuroscience   15 ( 1 II )   942 - 955   1995.1

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  • Glutathione-binding proteins identified by monoclonal antibodies which depress the behavioral response evoked by glutathione in Hydra Reviewed

    Kunimasa Ohta, Kazumitsu Hanai, Hiromichi Morita

    BBA - General Subjects   1117 ( 2 )   136 - 142   1992.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0304-4165(92)90071-2

  • Involvement of neuronal cell surface molecule B2 in the formation of retinal plexiform layers Reviewed

    Kunimasa Ohta, Shin Takagi, Hiroaki Asou, Hajime Fujisawa

    Neuron   9 ( 1 )   151 - 161   1992.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/0896-6273(92)90230-B

  • Monoclonal antibodies that depress a specific subset of multiple components of the glutathione-induced response of Hydra Reviewed

    Masahiko Sakaguchi, Kazumitsu Hanai, Kunimasa Ohta, Masaaki Kitajima, Sachiko Matsuhashi, Katsuji Hori, Hiromichi Morita

    Journal of Comparative Physiology A   168 ( 4 )   409 - 416   1991.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/BF00199601

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Books

Presentations

  • Bacterial ribosome induced multipotency in mouse fibrobkasts that platformed in vtro transdifferentiation to multilineage cells International conference

    Anamika Datta, Arif Istiaq, Kunimasa Ohta

    Society for Developmental Biology 83rd Annual Meeting  2024.7  Society for Developmental Biology

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    Event date： 2024.7

    Language：English   Presentation type：Poster presentation  

    Venue：Atlanta   Country：United States  

  • Mechanism of Exogenous Ribosome Mediated Cellular Multipotency Induction International conference

    Arif Istiaq & Kunimasa Ohta

    19th ISDB  2022.10 

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    Event date： 2022.10

    Language：English  

    Venue：Algarve   Country：Portugal  

  • INVOLVEMENT OF THE SOLUBLE MOLECULE, AKHIRIN, IN THE NICHE REGULATION DURING BRAIN DEVELOPMENT International conference

    @Kunimasa Ohta, #Tenta Ohkubo, and #Mikiko Kudo

    11th ICCNS  2022.10 

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    Event date： 2022.10

    Language：English   Presentation type：Oral presentation (general)  

    Venue：ニース   Country：France  

  • Lactic Acid Bacteria Converts Human Fibroblasts to Multipotent cells Invited International conference

    Kunimasa Ohta

    Kumamoto - NCBS/InStem Partnership Meeting  2019.3 

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    Event date： 2019.3

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Bangalore   Country：India  

  • Lactic acid bacteria convert human fibroblasts into multipotent cells Invited International conference

    Kunimasa Ohta

    The 6th Beneficial Microbes Conference  2017.10 

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    Event date： 2017.10

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Amsterdam   Country：Netherlands  

  • Tsukushiによる脳神経幹細胞の制御とリボソームによる新たな多能性細胞の創造 Invited

    太田訓正

    Wnt研究会2024  2024.11  日本Wnt研究会

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    Event date： 2024.11

    Language：Chinese   Presentation type：Oral presentation (invited, special)  

    Venue：福岡   Country：Japan  

  • リボソームは神経幹細胞を形質転換する

    コウ　コウケン,Datta Anamika, Arif Istiaq, 山下啓介、太田訓正

    第４７回日本分子生物学会年会  2024.11  日本分子生物学会

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    Event date： 2024.11

    Language：Japanese   Presentation type：Poster presentation  

    Venue：福岡  

  • 外来性リボソームはHEK293細胞の多能性を誘導する

    タン　フクチョウ、Datta Anamika, Arif Istiaq, 山下啓介、太田訓正

    第４７回日本分子生物学会年会  2024.11  日本分子生物学会

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    Event date： 2024.11

    Venue：福岡  

  • リボソームによるニワトリ初代筋由来細胞の形質転換

    井上翔太、Arif istiaq, Datta Anamika, Ikko Kawashima, Kunimasa Ohta

    ４７回日本分子生物学会年会  2024.11  日本分子生物学会

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    Event date： 2024.11

    Language：Japanese   Presentation type：Poster presentation  

    Venue：福岡  

  • バクテリア由来リボソームによる成体マウス線維芽細胞の形質転換

    Datta Anamika, Arif Istiaq, Kunimasa Ohta

    ４７回日本分子生物学会年会  2024.11  日本分子生物学会

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    Event date： 2024.11

    Language：English   Presentation type：Poster presentation  

    Venue：福岡  

  • 脳発生時における分泌型タンパク質Akhirinの機能解析

    工藤三希子、大久保天太、太田訓正

    第１５回日本ファミリーCCN研究会  2024.8  日本ファミリーCCN研究会

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    Event date： 2024.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学   Country：Japan  

  • Akhirin regulates the innate immune response during brain development and maintains homeostasis of the NSC Niche

    #工藤三希子、@太田訓正

    第１７回神経発生討論会  2024.3 

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    Event date： 2024.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋市立大学   Country：Japan  

  • Role of the secreted proteoglycan Tsukushi in brain development Invited International conference

    @Kunimasa Ohta

    Neural stem cell in brain development and disease  2023.9 

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    Event date： 2023.9

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Rome   Country：Italy  

  • 分泌型タンパク質Akhirinの脳発生時における機能解析

    #工藤三希子、#大久保天太、@太田訓正

    第１４回日本CCNファミリー研究会  2023.9 

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    Event date： 2023.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学   Country：Japan  

  • 分泌型タンパク質Akhirinによる脳神経幹細胞ニッチの制御

    #大久保天太、#工藤三希子、@太田訓正

    第４５回日本分子生物学会  2022.12 

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    Event date： 2022.11 - 2022.12

    Language：Japanese  

    Venue：幕張メッセ   Country：Japan  

  • Tsukushi proteoglycan dysfunction affects the splicing of neural stem/progenitor cells

    @Arif Istiaq, Terumasa Umemoto, Naofumi Ito, Toshio Suda, Kenji Shimamura, and @Kunimasa Ohta

    第４５回日本分子生物学会  2022.12 

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    Event date： 2022.11 - 2022.12

    Language：Japanese  

    Venue：幕張メッセ   Country：Japan  

  • Extrinsic ribosome stimuli promotes stem-cell like characteristics in mouse primary fibroblast

    #Anamika Datta, @Arif Istiaq, and @Kunimasa Ohta

    第４５回日本分子生物学会  2022.12 

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    Event date： 2022.11 - 2022.12

    Language：English  

    Venue：幕張メッセ   Country：Japan  

  • 分泌型タンパク質Akhirinによる神経幹細胞ニッチの制御

    #大久保天太、#工藤三希子、@太田訓正

    日本CCNファミリー研究会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • 分泌型タンパク質Akhirinによる脳神経幹細胞ニッチの制御

    大久保天太, 工藤三希子, 太田訓正

    日本CCNファミリー研究会  2022.9 

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    Event date： 2022.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    researchmap

  • Cellar dynamics in Exo-ribosome mediated multipotency induction in somatic cells International conference

    Arif Istiaq and @Kunimasa Ohta

    第3回日独合同若手ミーティング  2021.11 

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    Event date： 2021.11

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • Akhirin regulates neural stem cell proliferation through microglial activation International conference

    #Mikiko Kudo and @Kunimasa Ohta

    第3回日独合同若手ミーティング  2021.11 

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    Event date： 2021.11

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • DYSFUNCTION OF THE PROTEOGLYCAN TSUKUSHI CAUSES HYDROCEPHALUS THROUGH ALTERED NEUROGENESIS IN THE SUBVENTRICULAR ZONE IN MICE International conference

    @Kunimasa Ohta

    ISSCR/JSRM 2021 virtual Tokyo International Symposium  2021.10 

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    Event date： 2021.10

    Language：English  

    Venue：オンライン   Country：Japan  

  • Akhirin regulates neural stem cell proliferation through microglial activation

    #Kudo, M. & @Ohta, K.

    第６４回日本神経化学会大会  2021.9 

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    Event date： 2021.9 - 2021.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • 分泌型タンパク質Akhirinはミクログリアの活性化を介して神経幹細胞の増殖を制御する

    #工藤三希子、@太田訓正

    第１２回日本CCNファミリー研究会  2021.9 

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    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • リボソームによる細胞の形質転換 Invited

    @太田訓正、菅裕

    第３回再生学異分野融合研究会  2021.8 

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    Event date： 2021.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • Characterization of exogenious ribosome-mediated induced somatic cell multipotency

    Istiaq, A., #Kudo, M., #Takashi, K., #Haoxuan, G., #Fuchao, T., and @Ohta. K.

    第５４回日本発生生物学会  2021.6 

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    Event date： 2021.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • Functional analysis of the soluble protein Akhirin in the developing brain nerve stem cell niche

    #Kudo Mikiko, Arif Istiaq, and @Kunimasa Ohta

    第５４回日本発生生物学会  2021.6 

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    Event date： 2021.6

    Language：English  

    Venue：オンライン   Country：Japan  

  • Dysfunction of the proteoglycan Tsukushi causes hydrocephalus through altered neurogenesis in the subventricular zone in mice

    @Ohta, K., Ahmad, A., and Ito, N.

    幹細胞シンポジウム  2021.5 

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    Event date： 2021.5

    Language：English  

    Venue：オンライン   Country：Japan  

  • Disruption of Tsukushi function leads to the hydrocephalus by aberrent neurogenesis in the brain

    @Ohta, K., Adil, I., Anam, B., Ito, N.

    日本分子生物学会  2020.12 

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    Event date： 2020.12

    Language：English  

    Venue：オンライン   Country：Japan  

  • Intracellular interaction of bacterial ribosome in mammalian cell towards cellular transdifferentiation

    Istiaq, A., #Kuod, M., Nakayama, S., #Takashi, K., @Ohta, K.

    日本分子生物学会  2020.12 

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    Event date： 2020.12

    Language：English  

    Venue：オンライン   Country：Japan  

  • Ribosome incorporation induce EMT-like phenomenon with cell cycle arrest in human breast cancer cell

    #Kuod, M., Anam, B., Ahmad, S., Ito, N., @Ohta, K.

    日本分子生物学会  2020.12 

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    Event date： 2020.12

    Language：Japanese  

    Venue：オンライン   Country：Japan  

  • Dysfunction of Tsukushi function leads to the hydrocephalus by aberrant neurogenesis in the brain

    @Ohta, K., Ahmad, S., Anam, B., Ito, N.

    日本発生生物学会  2020.9 

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    Event date： 2020.9

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  • Incorporation of bacterial ribosome in diverse mammalian cells

    Istiaq, A., Urata, K., Nakamura, K., Anam, B., Ahmad, S.,# Kudo, M., Ito, N., @Ohta, K.

    日本発生生物学会  2020.9 

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    Event date： 2020.9

    Language：English  

    Venue：オンライン   Country：Japan  

  • Transdifferentiation of human cancer cells by ribosome

    #Kudo, M., Anam, B., Ahmad, S., Ito, N., @Ohta, K.

    日本発生生物学会  2020.9 

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    Event date： 2020.9

    Language：English  

    Venue：オンライン   Country：Japan  

  • Ribosome converts human fibroblasts to multipoint cells Invited International conference

    Kunimasa Ohta

    International Symposium Frontline in Developmental Biology  2018.3 

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    Event date： 2018.3 - 2018.5

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Kyoto   Country：Japan  

  • Ribosome Incorporation into Somatic Cells Promotes Reprogramming towards Multipotency without Activating Cell Proliferation Invited International conference

    Kunimasa Ohta

    Joint Meeting of the German and Japanese Society of Developmental Biologists  2017.3 

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    Event date： 2017.3

    Language：English   Presentation type：Oral presentation (general)  

    Venue：Kiel   Country：Germany  

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MISC

  • リボソームによる細胞の形質転換 Reviewed

    @太田訓正

    月刊「細胞」   2023.5

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Assessing the Role of Ependymal and Vascular Cells as Sources of Extracellular Cues Regulating the Mouse Ventricular-Subventricular Zone Neurogenic Niche. Reviewed

    Quaresima S, Istiaq A, Jono H, Cacci E, @Ohta K, Lupo G.

    Front Cell Dev Biol.   2022.4

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.3389/fcell.2022.845567

  • A review on Tsukushi: mammalian development, disorders, and therapy Reviewed

    Istiaq, A., @Ohta, K.

    Journal of Cell Communication and Signaling   2022.3

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1007/s12079-022-00669-z

  • プロテオグリカンTsukushiによる脳神経幹細胞ニッチの制御

    @太田訓正

    医学のあゆみ   2022.1

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • regulation of brain neural stem cell niche by proteoglycan Tsukushi Invited

    Kunimasa Ohta

    医学のあゆみ   280 ( 3 )   234 - 235   2022.1

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    Authorship：Lead author  

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  • Ribosome-Induced Cellular Multipotency, an Emerging Avenue in Cell Fate Reversal Reviewed

    Arif Istiaq and @Kunimasa Ohta

    Cells   2021.9

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    Other Link： https://doi.org/10.3390/cells10092276

    Repository Public URL： http://hdl.handle.net/2324/4771848

  • Regulation of the Brain Neural Niche by Soluble Molecule Akhirin Reviewed

    #Mikiko Kudo and @Kunimasa Ohta

    J. Developmental Biology   2021.7

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.3390/jdb9030029

    Other Link： https://www.mdpi.com/2221-3759/9/3/29/htm

    Repository Public URL： http://hdl.handle.net/2324/4774265

  • Bacteria to form induced pluripotent stem cells Reviewed

    Istiaq, A., Ahmad, S.A.I., Anam, M.B., #Kudo, M., #Nakayama, S., Ito, N., and @Ohta, K.

    Methods iniPSC Technology, Volume 9   2021.6

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 毛周期調節におけるTsukushiとTGFβシグナル

    工藤三希子、伊藤尚文、河野利恵、太田訓正

    毛髪科学の新展開、CMC出版   2019.9

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Transdifferentiation of human somatic cells by ribosome Reviewed

    Naofumi Ito, Mohammad Badrul Anam, Shah Adil Ishtiyaq Ahmad, Kunimasa Ohta

    Development Growth and Differentiation   2018.6

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1111/dgd.12538

    Repository Public URL： http://hdl.handle.net/2324/4776821

  • Involvement of Tsukushi in diverse developmental processes Reviewed

    Shah Adil Ishtiyaq Ahmad, Mohammad Badrul Anam, Naofumi Ito, Kunimasa Ohta

    Journal of Cell Communication and Signaling   2018.3

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1007/s12079-018-0452-8

  • 乳酸菌による細胞のリプログラミング

    伊藤尚文、太田訓正

    酵母菌、麹菌、乳酸菌の産業応用展開、、CMC出版   2018.1

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Regulation of the neural niche by the soluble molecule Akhirin Reviewed

    Uzzal Kumar Acharjee, Athary Abdulhaleem Felemban, Asrafuzzaman M. Riyadh, Kunimasa Ohta

    Development Growth and Differentiation   2016.6

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1111/dgd.12284

    Repository Public URL： http://hdl.handle.net/2324/4774266

  • Reprogramming of human somatic cells by bacteria Reviewed

    Naofumi Ito, Kunimasa Ohta

    Development Growth and Differentiation   2015.5

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1111/dgd.12209

    Repository Public URL： http://hdl.handle.net/2324/4776819

  • Multiple roles of Equarin during lens development Reviewed

    Xiaohong Song, Hideaki Tanaka, Kunimasa Ohta

    Development Growth and Differentiation   2014.4

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.1111/dgd.12121

  • Neuronal stem/progenitor cells in the vertebrate eye Reviewed

    Kunimasa Ohta, Ayako Ito, Hideaki Tanaka

    Development Growth and Differentiation   2008.5

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    Language：English  

    DOI： 10.1111/j.1440-169X.2008.01006.x

  • Molecular mechanism of vertebrate eye formation Reviewed

    Kunimasa Ohta

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   2007.10

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    Language：English  

  • Orienting axon growth Spinal nerve segmentation and surround-repulsion Reviewed

    David Tannahill, Joanne M. Britto, Matthieu M. Vermeren, Kunimasa Ohta, Geoffrey M.W. Cook, Roger J. Keynes

    International Journal of Developmental Biology   2000.3

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    Language：English  

  • The inhibitory effect on neurite outgrowth of moroneurons exerted by the ligands ELF-1 and RAGS Reviewed

    Kunimasa Ohta, Hiroko Iwamasa, Uwe Drescher, Hidenori Terasaki, Hideaki Tanaka

    Mechanisms of Development   1997.6

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    Language：English  

    DOI： 10.1016/S0925-4773(97)00056-7

  • Commitment of motoneuron progenitors of chick embryo along the anterior-posterior axis Reviewed

    H. Tanaka, M. Fukushima, K. Ohta, Y. Kimura, A. Sudo, K. Shirabe, M. Takeshita

    Developmental Neuroscience   1997.1

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    Language：English  

    DOI： 10.1159/000111193

  • Function of a cell adhesion molecule, plexin, in neuron network formation Reviewed

    Hajime Fujisawa, Kunimasa Ohta, Toshiki Kameyama, Yasunori Murakami

    Developmental Neuroscience   1997.1

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    Language：English  

    DOI： 10.1159/000111192

  • The 3rd German-Japanese Joint Workshop. Cell Death in the CNS Molecules and Programs Reviewed

    Werner E.G. Müller, Francisco J. Romero, Gabriela Pergande, Sanja Perovic, Manuel B. Graeber, Siegfried Kösel, Rupert Egensperger, Nina M. Schnopp, Parviz Mehraein, Masayuki Miura, Masato Okada, Alexander Von Holst, Frances B. Lefcort, Alfredo Rodriguez-Tébar, Jean Jacques Michaille, Danielle Dhouailly, Anders Bäckström, Ted Ebendal, Hermann Rohrer, M. Sendtner, T. Michaelidis, C. Gravel, R. Götz, G. Ochs, K. V. Toyka, H. Thoenen, R. Egensperger, S. Kösel, P. Mehraein, M. B. Graeber, Shunsaku Homma, Ronald W. Oppenheim, Shin Kwak, Rolf Heumann, Frank Narz, Yildirim Algür, Daniela Bartsch, Martin Hüser, Franz Joseph Klinz, Erwin Wagner, Hartmut Berns, Kirstin Obst, Petra Wahle, H. Tanaka, M. Nakamura, M. Fukushima, K. Ohta, Leonard L. Jones, Richard B. Banati, Gennadij Raivich, Georg W. Kreutzberg, Christian Reiter, Zhiping Nie, Karl Friedrich Fischbach, Yutaka Eguchi, Shigeomi Shimizu, Yoshihide Tsujimoto, Alun M. Davies, Jimi Adu, Gayle Middleton, Yoshiyuki Kuchino, Chifumi Kitanaka, Akinori Sugiyama, Akio Asai, Ralf Gold, Hans Peter Hartung, Ichiro Matsuoka, Miwako Kobayashi, Miyuki Fujii, Kenzo Kurihara, Takashi Suda, Masato Tanaka, Masashi Adachi, Shigekazu Nagata, Seisuke Hattori, Michiyuki Matsuda, Shun Nakamura, Makoto Hamanoue, Mitsuru Machide, Kunio Matsumoto, Toshikazu Nakamura, Kazuyuki Nakajima, Shinichi Kohsaka, Akio Wanaka, Kazunori Imaizumi, Manabu Tsuda, Yuji Imai, Masaya Tohyama, Tsutomu Takagi, Y. A. Barde, Hans Georg Mannherz, Frank Rauch, Silvia Zanotti, Harald Stephan, Renate Paddenberg, Bernhard Polzar, Hartmut Engelmann, Sigrun Hess, Eva Gottfried

    Journal of Brain Research   1996.12

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    Language：English  

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Works

  • リボソームによる細胞リプログラミング機構の解析

    2013 - Present

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  • Tsukushiと水頭症発症の連関

    太田 訓正

    2012 - Present

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  • Akhirinによる神経幹細胞ニッチの制御

    2005 - Present

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Industrial property rights

Patent   Number of applications: 5   Number of registrations: 5
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • The Japan Neuroscience Society

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  • JAPANESE SOCIETY of DEVELOPMENTAL BIOLOGISTS

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  • The Molecular Biology Society of Japan

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  • The International CCN Society (ICCNS)

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  • INTERNATIONAL SOCIETY FOR STEM CELL RESEARCH (ISSCR)

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Committee Memberships

  • 神経発生討論会   世話人  

    2021.4 - Present   

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  • 日本CCNファミリー研究会   幹事  

    2020.8 - Present   

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  • 日本発生生物学会   幹事長   Domestic

    2020.4 - 2021.6   

  • The International CCN Society (ICCNS)   adviser  

    2014 - Present   

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    Committee type：Academic society

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Academic Activities

  • 学術論文等の審査 International contribution

    Role(s)： Review, evaluation,　Peer review

    2024

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    Type：Peer review 

  • Screening of academic papers

    Role(s)： Peer review

    2023

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：10

  • Screening of academic papers

    Role(s)： Peer review

    2022

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：12

  • Frontiers in Cell and Developmental Biology International contribution

    Role(s)： Review, evaluation,　Peer review

    2021.2 - Present

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    Type：Scientific advice/Review 

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  • Screening of academic papers

    Role(s)： Peer review

    2021

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：15

  • Frontiers in Cell and Developmental Biology International contribution

    2020.8 - Present

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    Type：Academic society, research group, etc. 

  • Cells (Special Issue) International contribution

    Role(s)： Review, evaluation,　Peer review

    2020.8 - Present

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    Type：Scientific advice/Review 

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  • Journal of Cell Communication and Signaling (Special Issue) International contribution

    Role(s)： Review, evaluation,　Peer review

    2020.8 - Present

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    Type：Scientific advice/Review 

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  • Journal of Cell Communication and Signaling (Special Issue) International contribution

    2020.8 - 2021.8

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    Type：Academic society, research group, etc. 

  • Cells (Special Issue) International contribution

    2020.8 - 2021.8

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    Type：Academic society, research group, etc. 

  • プログラム委員

    第５３回日本発生生物学会  （ Japan ） 2020.5

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    Type：Competition, symposium, etc. 

    Number of participants：450

  • Screening of academic papers

    Role(s)： Peer review

    2020

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：4

  • Screening of academic papers

    Role(s)： Peer review

    2019

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：5

  • 科研費審査委員

    Role(s)： Review, evaluation

    日本学術振興会  2017.4 - 2018.3

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    Type：Scientific advice/Review 

  • Journal of Cell Communication and Signaling International contribution

    2014.9 - Present

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    Type：Academic society, research group, etc. 

  • Journal of Cell Communication and Signaling International contribution

    Role(s)： Review, evaluation

    2014.9 - Present

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    Type：Peer review 

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  • Development Growth & Differentiation International contribution

    Role(s)： Peer review

    2010.4 - 2020.3

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    Type：Academic society, research group, etc. 

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Other

  • 水頭症は、脳室が拡大して、脳室内での脳脊髄液が停滞し、頭蓋骨内の圧力が高まることにより発症する難病指定の特定疾患です。特に、特発性正常圧水頭症は、歩行障害・精神疾患・尿失禁などの症状を呈し、高齢化社会を迎える日本における患者数増加が懸念されていますが、その発症要因が不明であることから、本疾患の新規予防・治療薬の開発は喫緊の社会的課題となっています。今回の研究では、水頭症発症のメカニズムの解明につながる重要な研究結果を、熊本大学をはじめとする国内外の12大学、5研究所との共同研究により、自らが単離・命名したTsukushi遺伝子が、水頭症の発症に関与していることを世界で初めて明らかにしました。

    2021.3

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    The lateral ventricle (LV) is flanked by the subventricular zone (SVZ), a neural stem cell (NSC) niche rich in extrinsic growth factors regulating NSC maintenance, proliferation and neuronal differentiation. Dysregulation of the SVZ niche causes LV expansion, a condition known as hydrocephalus; however, the underlying pathological mechanisms are unclear. We show that deficiency of the proteoglycan Tsukushi (TSK) in ependymal cells at the LV surface and in the cerebrospinal fluid results in hydrocephalus with neurodevelopmental disorder-like symptoms in mice. These symptoms are accompanied by altered differentiation and survival of the NSC lineage, disrupted ependymal structure and dysregulated Wnt signaling. Multiple TSK variants found in patients with hydrocephalus exhibit reduced physiological activity in mice in vivo and in vitro. Administration of wild-type TSK protein or Wnt antagonists, but not of hydrocephalus-related TSK variants, in the LV of TSK knockout mice prevented hydrocephalus and preserved SVZ neurogenesis. These observations suggest that TSK plays a crucial role as a niche molecule modulating the fate of SVZ NSCs and point to TSK as a candidate for the diagnosis and therapy of hydrocephalus.

Research Projects

  • Tsukushi KOマウスの脳室拡大に関与する脳脊髄液タンパク質の同定とその機能解析

    2025.4 - 2026.3

    熊本大学発生医学研究所 

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    Authorship：Principal investigator  Grant type：Joint research

  • 一次生産者が細胞を細胞農業業界へ販売する社会を実現するための基礎技術の開発 International coauthorship

    2025.4 - 2026.3

    インテグリカルチャー社 

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    Authorship：Principal investigator  Grant type：Joint research

  • リボソーム内における細胞多能性獲得を担うドメインの同定

    Grant number：2024-4112  2024.4 - 2024.9

    日本科学協会  笹川科学研究助成 

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    Authorship：Coinvestigator(s)  Grant type：Donation

  • 一次生産者が細胞を細胞農業業界へ販売する社会を実現するための基礎技術の開発

    2022.10 - 2024.11

    Joint research

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • Akhirinによるマウス脳神経幹細胞の制御

    2022.4 - 2023.3

    Joint research

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • Tsukushiによる脳神経幹細胞制御機構の解明 International coauthorship

    2021.10 - 2024.9

    イタリア 

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    Authorship：Principal investigator 

  • Akhirinによるマウス脳神経幹細胞の制御

    2021.4 - 2022.3

    Joint research

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • Transdifferentiation of unicellula organism to muticellular organism by ribosome

    Grant number：21K19271  2021 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

    Ohta Kunimasa

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    Authorship：Principal investigator  Grant type：Scientific research funding

    We have reported that ribosome can induce the transdifferentiation of host cells (Ito et al., 2018). In this project, we introduced ribosome into the Creolimax which has life cycles of unicellular organism and multicellular organism and observed the phenomenon after the ribosome incorporation.
    Normally, Creolimax can't survive in PBS buffer without any nutritions. However, we showed that Creolimax can survive in the presence of ribosome more than 6 months. When Creolimax incorporated the ribosome, it form the clusters similar to the cell clusters by incorporating ribosome into fibroblasts. We are planing to check the molecular changes in Creolimax which incorporated ribosome.

    CiNii Research

  • 大規模培養装置CulNet systemに用いる人工臓器部位作出

    2020.4 - 2021.5

    Joint research

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • Akhirinによる幹細胞ニッチ制御 International coauthorship

    2019.3

    九州大学 

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    Authorship：Principal investigator 

  • リボソームによる細胞のリプログラミング機構の解明

    Grant number：1068043  2019 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • リボソームによる細胞の形質転換機構

    2018.3

    九州大学 

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    Authorship：Principal investigator 

  • タンパク質合成装置リボソーム内に存在するリプログラミング因子の同定

    Grant number：1038445  2018 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • リボソームによる細胞のリプログラミング機構

    2017

    Global COE Program (Ministry of Education, Culture, Sports, Science and Technology)

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    Authorship：Principal investigator  Grant type：Contract research

  • リボソームによる細胞の多能性獲得機構の解明

    2016 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 乳酸菌由来リボソームによる細胞のリプログラム機構

    2016 - 2017

    公益財団法人発酵研究所　一般研究助成

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    Authorship：Principal investigator  Grant type：Contract research

  • リボソーム内に存在するリプログラミング機能ドメインの同定

    2016

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 脳・腸連関を支える自律神経系の理解から恒常性維持機構の解明へ

    2015 - 2019

    日本医療開発機構（AMED）

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    Authorship：Coinvestigator(s)  Grant type：Contract research

  • 幹細胞を用いた臓器再建と次世代医療・創薬を目指す国際的研究拠点

    2014 - 2019

    熊本大学　国際先端研究拠点　研究助成

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    Authorship：Coinvestigator(s)  Grant type：Contract research

  • 細胞形質転換に関わる乳酸菌由来因子の同定

    2013 - 2014

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 血管由来の細胞外因子による神経幹細胞の制御

    2010 - 2014

    Japan Society for the Promotion of Science・Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • リボソームによる細胞リプログラミング機構の解明

    Grant number：19H03235 

    太田 訓正, 嶋村 健児

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    Grant type：Scientific research funding

    乳酸菌からリボソームを精製し、細胞に取り込ませたところ、細胞塊が形成され、様々な多能性マーカーの発現を誘導し、三胚葉由来の細胞へと分化できたことから、リボソームがリプログラミング物質実体であることを報告した（Ito et al., 2018）。細胞運命の転換現象は明らかになったが、運命転換機構はまだ解明されていないことから、本研究においては、リボソーム誘導細胞塊の樹立課程を詳細に解析することによって、リボソームがどのような分子制御によって細胞に多分化能を付与するかを明らかにし、発生・進化における細胞分化の新たな意義を提唱することが、本研究課題の核心である。

    CiNii Research

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Educational Activities

  • 前期は、歯学部１年生に対して細胞生物学の授業を行なっている。
    夏期は、学部１年生向けの基幹教育セミナーを担当する。
    後期は、１年生に対して課題協学を担当する。
    秋期は、大学院生に対して幹細胞の授業を担当する。

Class subject

  • 課題協学科目

    2024.10 - 2025.3   Second semester

  • 生物科学IV

    2024.10 - 2024.12   Fall quarter

  • 生物科学Ⅱ

    2024.10 - 2024.12   Fall quarter

  • 生物科学特別講義

    2024.9   Fall quarter

  • 基幹教育セミナー

    2024.6 - 2024.8   Summer quarter

  • 細胞生物学

    2024.4 - 2024.8   First semester

  • Basic Biology II

    2023.10 - 2023.12   Fall quarter

  • 生物科学Ⅱ

    2023.10 - 2023.12   Fall quarter

  • 基幹教育セミナー

    2023.6 - 2023.8   Summer quarter

  • 細胞生物学

    2023.4 - 2023.9   First semester

  • Integrative Biology, Advanced CourseⅠ

    2023.4 - 2023.9   First semester

  • 統合生物科学特論Ⅰ

    2023.4 - 2023.9   First semester

  • 課題協学科目

    2022.10 - 2023.3   Second semester

  • 生物科学特論Ⅳ

    2022.10 - 2022.12   Fall quarter

  • Basic BiologyⅡ

    2022.10 - 2022.12   Fall quarter

  • 生物科学Ⅱ（※分子生命科学Ⅱ）

    2022.10 - 2022.12   Fall quarter

  • 生物科学Ⅱ（※生命理学Ⅱ）

    2022.10 - 2022.12   Fall quarter

  • 基幹教育セミナー

    2022.6 - 2022.8   Summer quarter

  • 細胞生物学

    2022.4 - 2022.9   First semester

  • 課題協学科目

    2021.10 - 2022.3   Second semester

  • Basic BiologyⅡ

    2021.10 - 2021.12   Fall quarter

  • 生物科学Ⅱ

    2021.10 - 2021.12   Fall quarter

  • 基幹教育セミナー

    2021.6 - 2021.8   Summer quarter

  • 統合生物科学特論Ⅰ

    2021.4 - 2021.9   First semester

  • 細胞生物学

    2021.4 - 2021.9   First semester

  • Integrative Biology, Advanced CourseⅠ

    2021.4 - 2021.9   First semester

  • 課題協学科目

    2020.10 - 2021.3   Second semester

  • Biological Sciences, Advanced Course Ⅰ

    2020.10 - 2020.12   Fall quarter

  • 生命理学特論Ⅰ

    2020.10 - 2020.12   Fall quarter

  • 基幹教育セミナー

    2020.6 - 2020.8   Summer quarter

  • 細胞生物学

    2020.4 - 2020.9   First semester

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FD Participation

  • 2023.5   Role：Moderator   Title：基幹教育セミナーFD

    Organizer：University-wide

  • 2022.5   Role：Moderator   Title：基幹教育セミナーFD

    Organizer：University-wide

  • 2021.5   Role：Participation   Title：基幹教育セミナーFD

    Organizer：University-wide

  • 2021.3   Role：Participation   Title：オンライン授業におけるアクティブラーニングの科目の実施状況：アクティブラーニングの今後を考える

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.3   Role：Participation   Title：Teamsによるonline会議

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.6   Role：Participation   Title：基幹教育セミナー　オンラ イン授業の『手引き』

    Organizer：University-wide

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Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2022  岡山大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2021  熊本大学大学院生命科学研究部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2020  名古屋大学大学院  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2020  県立広島大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2016  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2015  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2014  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2013  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2012  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2011  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2010  大分大学医学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

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Other educational activity and Special note

  • 2022  Coaching of Students' Association  課外活動支援専門部会

    　詳細を見る

    委員

  • 2021  Class Teacher  学部

  • 2020  Class Teacher  学部

Outline of Social Contribution and International Cooperation activities

  • I gave a lecture to the high school students (Sagamihara) about the stem cell biology.

Social Activities

  • 様々な幹細胞の研究/私たちが研究している脳神経幹細胞や、ヒト皮膚細胞にリボソームを取り込ませて作成した多能性細胞の特徴を説明した。

    神奈川県立相模原高等学校  2022.9

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    Audience：Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

  • SSHとは、高等学校等において、先進的な理数教育を実施し、高大接続の在り方について大学との共同研究や、国際性を育むための取組である。SSH指定校である熊本県立宇土高校から、過去５年間、毎年数名の高校生が私たちの研究室を訪問し、細胞培養等の実験を行ってきた。

    熊本県立宇土高校  2020.3

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    Audience：Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

  • スーパーサイエンスハイスクール指定校である宇土高校２年生を対象に、５年間、細胞培養等の実験指導を行った。

    熊本県立宇土中学校・宇土高等学校  熊本大学大学院・生命科学研究部  2018.5

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    Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Research consultation

    SSHとは、高等学校等において、先進的な理数教育を実施し、高大接続の在り方について大学との共同研究や、国際性を育むための取組である。SSH指定校である熊本県立宇土高校から、毎年数名（2016年2名、2017年3名、2018年3名、2019年4名）の高校生が私たちの研究室を訪問し、細胞培養等の実験を行ってきた。

Media Coverage

  • 水頭症　遺伝子の変異確認　九州大、治療法開発に期待 Newspaper, magazine

    熊本日日新聞　他30社  2021.4

    　More details

    水頭症　遺伝子の変異確認　九州大、治療法開発に期待

  • リボソーム細胞を初期化 Newspaper, magazine

    時事通信社  2018.1

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    リボソーム細胞を初期化

  • 乳酸菌で細胞を初期化、リボソームが関与 Newspaper, magazine

    日本経済新聞  2018.1

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    乳酸菌で細胞を初期化、リボソームが関与

  • リボソーム細胞万能化 Newspaper, magazine

    熊本日日新聞  2018.1

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    リボソーム細胞万能化

  • SSH指定校の宇土高校 Newspaper, magazine

    熊本日日新聞  2016.3

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    SSH指定校の宇土高校

  • 今さら聞けない乳酸菌 Newspaper, magazine

    朝日新聞  2015.1

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    今さら聞けない乳酸菌

  • 乳酸菌で多能性細胞 Newspaper, magazine

    化学工業日報  2013.9

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    乳酸菌で多能性細胞

  • がん化しにくい多能性細胞を作製 Newspaper, magazine

    日本経済新聞  2012.12

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    がん化しにくい多能性細胞を作製

  • 乳酸菌使い多能性細胞 Newspaper, magazine

    熊本日日新聞  2012.12

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    乳酸菌使い多能性細胞

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Acceptance of Foreign Researchers, etc.

  • ローマ・サピエンザ大学

    Acceptance period： 2022.12   （Period）：2weeks to less than 1 month

    Nationality：Italy

    Business entity：Foreign governments, foreign research institutes, international organizations

  • ローマ・サピエンザ大学

    Acceptance period： 2022.12   （Period）：2weeks to less than 1 month

    Nationality：Italy

    Business entity：Foreign governments, foreign research institutes, international organizations

  • （Period）：1 month or more

    Business entity：Foreign governments, foreign research institutes, international organizations

  • （Period）：1 month or more

    Business entity：On-campus funds

  • （Period）：1 month or more

    Business entity：Private/Foundation

  • （Period）：1 month or more

    Business entity：On-campus funds

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Travel Abroad

  • 2022.11 - 2022.12

    Staying countory name 1：Italy   Staying institution name 1：SAPIENZA UNIVERSITY OF ROME

  • 2022.10

    Staying countory name 1：Portugal   Staying institution name 1：2022ISDB

  • 2022.10

    Staying countory name 1：France   Staying institution name 1：International CCN Society

  • 2019.10

    Staying countory name 1：Canada   Staying institution name 1：International CCN Society

  • 2019.4

    Staying countory name 1：France   Staying institution name 1：2019 Neurogenesis

  • 2019.3

    Staying countory name 1：India   Staying institution name 1：Nationcal Center for Biological Sciences

  • 2018.10

    Staying countory name 1：Italy   Staying institution name 1：International Society for Hydrocephalus

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