記述言語：英語   出版者・発行元：Ophthalmology Science  

Purpose: To compare the usefulness of microperimetry and static automated perimetry in patients with retinitis pigmentosa (RP), using macular anatomical metrics as a reference. Design: Prospective observational study. Participants: Forty-eight eyes of 48 patients with RP in Kyushu University Hospital who underwent microperimetry-3 (MP-3) and Humphrey Field Analyzer (HFA) 10-2 testing ≥3 times during ≥2 years were included. Methods: Macular anatomy (ellipsoid zone [EZ] length) was assessed by OCT, and macular function was assessed by MP-3 (mean retinal sensitivity at radii 2°, 4°, and 8°) and HFA10-2 program (mean retinal sensitivity at radii 2°, 4°, and 8°). Correlations between functional and anatomical parameters were analyzed cross sectionally at baseline and longitudinally by comparing the rate of progression. Main Outcome Measures: Correlation coefficients between anatomical and functional metrics. Results: The mean age at baseline was 50.1 ± 12.3 years, and the mean follow-up period was 2.8 ± 0.7 years. At baseline, EZ length was significantly correlated with MP-3 mean retinal sensitivity at radii 2°, 4°, and 8° (Spearman's ρ = 0.65, 0.84, 0.89; all P < 0.005) and HFA10-2 mean retinal sensitivity at radii 2°, 4°, and 8° (Spearman's ρ = 0.61, 0.73, 0.78; all P < 0.005). Longitudinal analysis showed that the slope of EZ length (−88.92 μm/year) was significantly correlated with the slope of MP-3 retinal sensitivity at 8° radius (−0.62 decibels [dB]/year; Spearman's ρ = 0.31, P=0.03) and the slope of HFA retinal sensitivity at 8° radius (−0.60 dB/year; Spearman's ρ = 0.43, P < 0.005). Conclusions: Both MP-3 and HFA values were cross sectionally well-correlated with EZ length in patients with patients; however, these associations became weaker in the longitudinal analysis. This highlights the need for researchers to explore additional or more sensitive parameters to better monitor RP progression. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.

DOI： 10.1016/j.xops.2024.100582

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PubMed

記述言語：英語   出版者・発行元：Japanese Journal of Ophthalmology  

Purpose: Extracellular Adenosine triphosphate (ATP) released by dying cells may cause a secondary cell death in neighboring cells in retinal degeneration. We investigated intraocular ATP kinetics to gain mechanical insights into the pathology in rhegmatogenous retinal detachment (RRD). Study design: Retrospective clinical study. Methods: Vitreous or subretinal fluids (SRF) were obtained from patients with RRD (n=75), macular hole (MH; n=20), and epiretinal membrane (ERM; n=35) during vitrectomy. ATP levels in those samples were measured by luciferase assay. Results: Mean ATP levels in the vitreous from RRD patients were significantly higher compared to those from MH and ERM patients (2.3 and 0.3 nM, respectively. P<0.01). Mean ATP levels in the SRF from RRD (11.7 nM) were higher than those in the vitreous from RRD (P<0.01). Mean ATP levels in the vitreous with short durations (1–8 days) of RRD were higher compared to those with long durations (>8 days) (3.2 and 1.4 nM, respectively. P<0.05). Similarly, ATP in SRF with short durations were higher than those with long durations (23.8 and 3.6 nM, respectively. P<0.05). Furthermore, the concentrations of ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), a major ATP degradative enzyme, in the vitreous from RRD were higher than those from MH/ERM (1.2 and 0.2 ng/ml, respectively. P<0.01). ENTPD1 expression was localized in the cytoplasm of CD11b-positive infiltrating cells in the vitreous and retinal cells. Conclusion: ATP increased in the vitreous and SRF in RRD and decreased over time with an upregulation of ENTPD1. The kinetics indicate the pathological mechanism of the excessive extracellular ATP after RRD.

DOI： 10.1007/s10384-024-01087-x

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PubMed

記述言語：英語   出版者・発行元：シュプリンガー・ジャパン(株)  

裂孔原性網膜剥離(RRD)にみられる眼内アデノシン三リン酸(ATP)の動態を検討した。RRD患者75例、黄斑円孔(MH)患者20例および網膜上膜(ERM)患者35例から硝子体切除中に硝子体液または網膜下液(SRF)を採取してATP濃度を測定した。RRD群の硝子体中ATP値は2.28±4.27nMであり、MH/ERM群(0.30±0.65nM)より有意に高く、SRF中ATP値は11.7±25.1nMと硝子体中濃度と比較して有意に高値を示していた。RRD群を罹病期間1～8日の32例と8日超の32例に分けると、罹病期間短期群の方が硝子体中・SRF中ATP値は高くなっていた。また、硝子体中ATPの分解能はRRD群の方がMH/ERM群より有意に高く、RRD群のアデノシン二リン酸(ADP)値はMH/ERM群より高かったことから、過剰なATPに対して酵素的分解が生じていると考えられた。さらに、RRD群でエクトヌクレオチダーゼとしてのENTPD1の濃度を測定したところ、罹病期間短期群と長期群との間に有意差はみられず、ENTPD1はRRDの発症直後に発現を呈して長期間にわたり維持されると考えられ、ENTPD1は硝子体細胞と網膜細胞の細胞質内に局在していた。ATPはRRD患者の硝子体とSRFにおいて高発現を呈し、ENTPD1のアップレギュレーションに伴って経時的に減少していた。

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記述言語：日本語  

記述言語：英語  

記述言語：日本語   出版者・発行元：金原出版(株)