Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Analytical Chemistry  

Fourier transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy is a powerful spectroscopic method that combines the principles of Fourier transform spectroscopy with coherent anti-Stokes Raman scattering (CARS). This method stands out in spectroscopy for its ability to rapidly acquire coherent Raman spectra, achieving an impressive rate of over 10 000 spectra per second. The method involves scanning the optical delay between two femtosecond pulses; the initial pulse induces a vibrational coherence in the sample, while the subsequent pulse probes this coherence over increasing delays. The anti-Stokes scattering intensity generated is modulated by the vibrational dynamics of the sample, enabling the retrieval of Raman spectra through Fourier transformation. Over the past two decades, FT-CARS spectroscopy has undergone substantial evolution, paving the way for its application in a wide array of fields, including material analysis and flow cytometry. In this comprehensive Review, we explore the fundamental principles and diverse applications of FT-CARS spectroscopy and delve into the potential future advances and challenges associated with this emerging method.

DOI： 10.1021/acs.analchem.4c02645

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Journal of Physical Chemistry Letters  

Fluorescence-encoded vibrational spectroscopy has attracted increasing attention by virtue of its high sensitivity and high chemical specificity. We recently demonstrated fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS), which enables low-frequency vibrational spectroscopy of low-concentration fluorophores using near-infrared (800-900 nm) light excitation. However, the feasibility of this study was constrained by the scarcity of excitable molecules in the near-infrared range. Consequently, the broader applicability of FLETCHERS has not been investigated. Here we extend the capabilities of FLETCHERS into the visible range by employing a noncollinear optical parametric amplifier as a light source, significantly enhancing its versatility. Specifically, we use the method, which we refer to as visible FLETCHERS (vFLETCHERS), to individually acquire Raman spectra from five visible fluorophores that have absorption peaks in the 600-700 nm region. These results not only confirm the versatility of vFLETCHERS for a wide range of molecules but also allude to its widespread applicability in biological research through highly sensitive supermultiplexed imaging.

DOI： 10.1021/acs.jpclett.4c00817

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Language：Japanese   Publisher：(一社)日本生物物理学会