| 1. |
Chiwaka Kimura, Syuhei Konishi, Maki Hasegawa, Masahiro Oike, Development of vascular smooth muscle contractility by endothelium-derived transforming growth factor beta proteins, Pflugers Arch, European Journal of Physiology, 466, 2, 369-380, 2014.02, It is well established that the release of vasodilators and vasoconstrictors from vascular endothelium regulates vascular smooth muscle contraction. In this report, we investigate the role of the endothelium in the development and maintenance of constitutive vascular contractility. For that purpose, contractile activity of cultured bovine aortic smooth muscle cells (BASMCs) embedded in collagen gels was monitored by changes in gel diameter. After culturing for 5 days, ATP- and high KCl solution-induced contractions were significantly enhanced in the gels that were overlaid with bovine aortic endothelial cells (BAECs) or were cultured with conditioned medium of cultured BAECs. ATP-induced Ca(2+) transients, recorded in BASMCs cultured with conditioned medium of BAECs, were markedly augmented, but high KCl-induced Ca(2+) transients were not affected. BASMCs in control gels were spindle shaped, and those in endothelium-treated gels were more elongated and interconnected. The endothelial conditioned medium also strongly affected the intracellular distribution of actin fibers. Conditioned medium of BAECs contained TGFbeta1 and TGFbeta2. The TGFbeta receptor antagonist SB431542 as well as simultaneous treatment with TGFbeta1 and TGFbeta2 neutralizing antibodies completely reversed the above effects of endothelial conditioned medium on BASMCs. BAECs medium induced phosphorylation of Smad2 and increased ATP-induced phosphorylation of myosin light chain in BASMCs. The present results indicate that the release of TGFbeta1 and TGFbeta2 from vascular endothelium affects the contractility of vascular smooth muscle cells by altering their morphology and agonist-induced Ca(2+) mobilization.. |
| 2. |
Chiwaka Kimura, Masayuki Hayashi, Yuri Mizuno, Masahiro Oike, Endothelium-dependent epithelial-mesenchymal transition of tumor cells: Exclusive roles of transforming growth factor β1 and β2., Biochim Biophys Acta, 1830, 10, 4470-4481, 2013.10. |
| 3. |
Yuko Matsunaga, Satoru Fukuyama, Toshiaki Okuno, Fumiyuki Sasaki, Takehiko Matsunobu, Yukari Asai, Koichiro Matsumoto, Kazuko Saeki, Masahiro Oike, Yukari Sadamura, Kentaro Machida, Yoichi Nakanishi, Masato Kubo, Takehiko Yokomizo, Hiromasa Inoue, Leukotriene B4 receptor BLT2 negatively regulates allergic airway eosinophilia., FASEB Journal, 27, 8, 3306-3314, 2013.08. |
| 4. |
Kenji Miki, Hiromitsu Tanaka, Yoko Nagai, Chiwaka Kimura, Masahiro Oike, Transforming growth factor β1 alters calcium mobilizing properties and endogenous ATP release in A549 cells: possible implications for cell migration., Journal of Pharmacological Sciences, 113, 4, 113, 4, 387-394, 2010.08. |
| 5. |
Braunstein TH, Inoue R, Cribbs L, Oike M, Ito Y, Holstein-Rathlou N-H, Jensen LJ., The role of L- and T-type calcium channels in local and remote calcium responses in rat mesenteric terminal arterioles, Journal of Vascular Research, 46(2):138-151, 2009.02. |
| 6. |
Koyama T, Kimura C, Hayashi M, Watanabe M, Karashima Y, Oike M, Hypergravity induces ATP release and actin reorganization via tyrosine phosphorylation and RhoA activation in bovine endothelial cells., Pflugers Archiv - European Journal of Physiology, 457(4):711-719, 2009.02. |
| 7. |
Kimura C, M Oike, Heparan sulfate proteoglycan is essential to thrombin-induced calcium transients and NO production in aortic endothelial cells., Thrombosis and Haemostasis, 100(3):483-488, 2008.09. |
| 8. |
M Oike, Watanabe M, Kimura C, Involvement of heparan sulfate proteoglycan in sensing hypotonic stress in bovine aortic endothelial cells., Biochimica et Biophysica Acta, 1780(10):1148-1155, 2008.10. |
| 9. |
Ohta Y, Hayashi M, Kanemaru T, Abe K, Ito Y, Oike M., Dual modulation of airway smooth muscle contraction by Th2 cytokines via matrix metalloproteinase-1 production. , Journal of Immunology, 180(6):4191-4199, 2008.03. |
| 10. |
Watanabe M, Oike M, Ohta Y, Ito Y, Long-term treatment with TGFb1 impairs mechanotransduction in bovine aortic endothelial cells., British Journal of Pharmacology, 150(4):424-433, 2007.01. |
| 11. |
Kimura C, Oike M, Watanabe M, and Ito Y, Proapoptotic nitric oxide production in amyloid beta protein-treated cerebral microvascular endothelial cells, Microcirculation, 14(2):89-97, 2007.02. |
| 12. |
Watanabe M, Oike M, Ohta Y, Nawata H, Ito Y, Sustained contraction and loss of NO production in TGFb1-treated endothelial cells., British Journal of Pharmacology, 149(4):355-64, 2006.09. |
| 13. |
Hirakawa M, Oike M, Watanabe M, Karashima Y, Ito Y, Pivotal role of integrin alpha5beta1 in hypotonic stress-induced responses of human endothelium., FASEB Journal, 20(12):1992-1999, 2006.12. |
| 14. |
Cheng W, Oike M, Hirakawa M, Ohnaka K, Koyama T, Ito Y, Excess l-arginine restores endothelium-dependent relaxation impaired by monocrotaline pyrrole, Toxicology and Applied Pharmacology, 10.1016/j.taap.2005.01.002, 207, 3, 187-194, 207(3):187-94, 2005.09. |
| 15. |
Harada K, Takeuchi H, Oike M, Matsuda M, Kanematsu T, Yagisawa H, Nakayama K.-I., Maeda K, Erneux C, Hirata H, Role of PRIP-1, a novel Ins(1,4,5)P3 binding protein, in Ins(1,4,5)P3-mediated Ca2+ signaling, Journal of Cellular Physiology, 10.1002/jcp.20136, 202, 2, 422-433, 222(2):422-433, 2005.02. |
| 16. |
Hirakawa M, Oike M, Karashima Y, Ito Y, Sequential activation of RhoA and FAK/paxillin leads to ATP release and actin reorganization in human endothelium, Journal of Physiology, 10.1113/j.physiol.2004.065334, 558, 2, 479-488, 558(Pt2):479-488, 2004.07. |
| 17. |
Kimura C, Oike M, Ohnaka K, Nose Y, Ito Y., Constitutive nitric oxide production in bovine aortic and brain microvascular endothelial cells: a comparative study., Journal of Physiology, 10.1113/jphysiol.2003.057059, 554, 3, 721-730, 554(Pt3):721-30., 2004.02. |
| 18. |
Sakai J, Oike M, Hirakawa M, Ito Y., Theophylline and cAMP inhibit lysophosphatidic acid-induced hyperresponsiveness of bovine tracheal smooth muscle cells., Journal of Physiology, 10.1113/jphysiol.2003.039024, 549, 1, 171-180, 549(Pt1):171-80, 2003.05. |
| 19. |
Koyama T, Kimura C, Park SJ, Oike M, Ito Y., Functional implications of Ca2+ mobilizing properties for nitric oxide production in aortic endothelium., Life Sicences, 10.1016/S0024-3205(02)02246-4, 72, 4-5, 511-520, 72(4-5):511-20., 2002.12. |
| 20. |
Karashima Y, Oike M, Takahashi S, Ito Y, Propofol prevents endothelial dysfunction induced by glucose overload., British Journal of Pharmacology, 10.1038/sj.bjp.0704912, 137, 5, 683-691, 137(5):683-91, 2002.11. |
| 21. |
Hisadome K, Koyama T, Kimura C, Droogmans G, Ito Y, Oike M., Volume-regulated anion channels serve as an auto/paracrine nucleotide release pathway in aortic endothelial cells., Journal of General Physiology, 10.1085/jgp.20028540, 119, 6, 511-520, 119(6):511-20, 2002.07. |
| 22. |
Kimura C, Cheng W, Hisadome K, Wang YP, Koyama T, Karashima Y, Oike M, Ito Y., Superoxide anion impairs contractility in cultured aortic smooth muscle cells., American Journal of Physiology, 10.1152/ajpheart.00574.2001, 283, 1, H382-H390, 283(1):H382-90., 2002.06. |
| 23. |
Hirakawa M, Oike M, Masuda K, Ito Y., Tumor cell apoptosis by irradiation-induced nitric oxide production in vascular endothelium., Cancer Research, 62, 5, 1450-1457, 62(5):1450-7, 2002.03. |
| 24. |
Kimura C, Oike M, Koyama T, Ito Y., Alterations of Ca2+ mobilizing properties in migrating endothelial cells., American Journal of Physiology, 281, 2, H745-H754, 281(2):H745-54, 2001.08. |
| 25. |
Koyama T, Oike M, Ito Y., Involvement of Rho-kinase and tyrosine kinase in hypotonic stress-induced ATP release in bovine aortic endothelial cells., Journal of Physiology, 10.1111/j.1469-7793.2001.0759e.x, 532, 3, 759-769, 532(Pt 3):759-69., 2001.05. |
| 26. |
Kimura C, Oike M, Koyama T, Ito Y., Impairment of endothelial nitric oxide production by acute glucose overload., American Journal of Physiology, 280, 1, E171-E178, 280(1):E171-8., 2001.01. |
| 27. |
Kimura C, Oike M, Ito Y., Hypoxia-induced alterations in Ca2+ mobilization in brain microvascular endothelial cells., American Journal of Physiology, 279, 5, H2310-H2318, 279(5):H2310-8., 2000.11. |
| 28. |
Kimura C, Koyama T, Oike M, Ito Y., Hypotonic stress-induced NO production in endothelium depends on endogenous ATP., Biochemical and Biophysical Research Communications, 10.1006/bbrc.2000.3205, 274, 3, 736-740, 274(3):736-40., 2000.08. |
| 29. |
Oike M, Kimura C, Koyama T, Yoshikawa M, Ito Y., Hypotonic stress-induced dual Ca2+ responses in bovine aortic endothelial cells., American Journal of Physiology, 279, 2, H630-H638, 279(2):H630-8., 2000.08. |
| 30. |
Koyama T, Oike M, Komiyama S, Ito Y., Superoxide anion impairs Ca2+ mobilization in cultured human nasal epithelial cells., American Journal of Physiology, 277, 6, L1089-L1095, 277(6 Pt 1):L1089-95., 1999.12. |
| 31. |
Kimura C, Oike M, Ito Y., Acute glucose overload abolishes Ca2+ oscillation in cultured endothelial cells from bovine aorta: a possible role of superoxide anion., Circulation Research, 82, 6, 677-685, 82(6):677-85., 1998.04. |
| 32. |
Kimura C, Oike M, Kashiwagi S, Ito Y., Effects of acute glucose overload on histamine H2 receptor-mediated Ca2+ mobilization in bovine cerebral endothelial cells., Diabetes, 10.2337/diabetes.47.1.104, 47, 1, 104-112, 47(1):104-12., 1998.01. |
| 33. |
Oike M, Creed KE, Onoue H, Tanaka H, Ito Y., Increase in calcium in smooth muscle cells of the rabbit bladder induced by acetylcholine and ATP., Journal of Autonomic Nervous System, 10.1016/S0165-1838(98)00023-X, 69, 2-3, 141-147, 69(2-3):141-7., 1988.04. |
