


Tamotsu Kiyoshima | Last modified date:2022.04.23 |

Professor /
Maxillofacial Diagnostic & Surgical Sciences
Department of Dental Science
Faculty of Dental Science
Department of Dental Science
Faculty of Dental Science
Graduate School
Undergraduate School
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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/tamotsu-kiyoshima
Reseacher Profiling Tool Kyushu University Pure
Phone
092-642-6328
Fax
092-642-6329
Academic Degree
D.D.S., Ph.D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Morphological Oral Biology, Oral pathology
Total Priod of education and research career in the foreign country
02years01months
Outline Activities
1.Regeneration of the tooth
Three approaches as follows is aimed at the regeneration of the tooth.
1) Elucidation of the mechanism in the odontogenesis
2) Analysis the tissue-specific mechanism in aging process
3) Isolation and identification of the tissue-specific stem cells
2.Analysis of differences between oral cancers through cellular and molecular biological approaches
This research is aimed at the development of the new gene therapy method for oral cancer using the gene considered to relate to the profiles of oral cancers.
(Undergraduate School)
Oral pathology: (Lecture) The generalities and the particulars
(Exercise) The explanation of the pathological specimens meeting each unit of a lecture, and the instruction of the observation under a microscope
Outline of the dental and medical science: Introduction of the dental science
Comprehensive dental science
Research exposure: For 3rd and sixth grades
Inspection and exercise are performed to the student who expects experience of research activities in the spring and/or summer vacations, within limited condition that is fully taken the safe side into consideration.
(Graduate School)
The graduate students will study a part of above-mentioned research and write papers on it under instruction to be a "researcher" in the future. Moreover, they will be involved also in autopsy or pathological diagnosis of the oral disease from the department of medical examination so as to understand the fundamentals of pathology.
Research
Research Interests
Membership in Academic Society
- Molecular and genetic investigation on the development and regeneration of the tooth
keyword : regeneration of the tooth, tooth development, odontogenesis, epithelial-mesenchymal interaction
2000.01Vertebrate organs are typically composed of epithelial and mesenchymal tissues. Signaling between these tissues governs many aspects of odontogenesis, from the initiation of organ development to the terminal differentiation of organ specific cell types. As well as many other embryonic organs, the development and differentiation of the tooth germ depends on such inductive interactions. We previously demonstrated that odontogenesis occurs between embryonic day 10.5 (E10.5) and E12. Based on the histological findings, we performed cDNA subtraction between the E10.5 and E12 mandibles to detect any differentially expressed genes which might be involved in the initiation of odontogenesis. By sequencing, homology search and semi-quantitative RT-PCR, we found about twenty differentially expressed genes in the course of the early developmental stage of the mouse mandible. These genes are functionally related to protein transport, signal transduction, transcription, translation and molecular chaperon activity. To elucidation of the mechanism in the odontogenesis, we are going to characterize these functions with in situ hybridization analyses, analyses of protein interactions (yeast two-hybrid system and reporter assay) and so on. Taken together with data of analysis the tissue-specific mechanism in aging process and isolation/identification of the tissue-specific stem cells, development of method for tooth regeneration is undertaken.. - Age-dependent change of the periodontal tissue
keyword : tooth regeneration, periodontal tissue, aging, cell scenesence, protein interaction
2000.01. - Analysis of differentially expressing genes among oral squamous cell carcinomas and gene-associated functions
keyword : oral carcinoma, invasion, metastasis, transcriptional regulation, Protein-Protein Interaction
2000.01We previously established a human carcinoma cell line, MISK81-5 from a metastatic lymph node of oral squamous cell carcinoma. The sMISK was derived from MISK81-5 as a subpopulation having higher tumorigenicity. The analysis of differences between characteristics of the MISK81-5 cell line and its subpopulation, sMISK will be useful for studying the biological behavior of oral squamous cell carcinoma (OSCC). We then performed cDNA subtraction between these cells to detect differentially expressed genes, some of which might be involved in the biological behavior of OSCC. By sequencing, homology search and semi-quantitative RT-PCR, we found differentially expressed genes. These genes are functionally involved in signal transduction, transcription, translation and chromatin reorganization. Compared with those genes expressed in other OSCC and gastric carcinoma cell lines and keratinocytes, expressing pattern in some of these genes was interesting. To characterize the mechanism of carcinogenesis utilized by recombinant proteins based on these genes, we are going to analyze the ability of the proteins to effect on the physical profiles of the OSCC, identify the putative proteins to interact with recombinant proteins, and characterize their promoters with mutational analysis. This research is aimed at the identification of the target genes for the development of the new gene therapy method for oral cancer..
Papers
Presentations
- Japanese Dental Education Association
- Japanese Stomatological Society
- The Japanese Society of Conservative Dentistry
- Japanese Society of Pediatric Oral and Maxillofacial Surgery
- The Japanese Society of Pathology
- The Japanese Society for Oral Pathology
- Japanese Cancer Association
- Japanese Society for Oral Tumors
- Japanese Society of Oral and Maxillofacial Surgeon
- Japanese Association for Oral Biology
- The Japanese Society for the Temporomandibular Joint
Educational
Educational Activities
Pathology/Oral pathology: (Lecture/Exercise) The explanation of the typical pathological specimens based on the lecture, and the instruction of the observation under a microscope
Comprehensive dental science
Research exposure: For fourth and sixth grades, the instruction and the exercise of the molecular biological examination concerning the above-mentioned research as well as pathological diagnosis.
The graduate students will study a part of above-mentioned research and write papers on it under instruction to be a "researcher" in the future. Moreover, they will be involved also in autopsy or pathological diagnosis of the oral disease from the department of medical examination so as to understand the fundamentals of pathology.
Other Educational Activities
Pathology/Oral pathology: (Lecture/Exercise) The explanation of the typical pathological specimens based on the lecture, and the instruction of the observation under a microscope
Comprehensive dental science
Research exposure: For fourth and sixth grades, the instruction and the exercise of the molecular biological examination concerning the above-mentioned research as well as pathological diagnosis.
The graduate students will study a part of above-mentioned research and write papers on it under instruction to be a "researcher" in the future. Moreover, they will be involved also in autopsy or pathological diagnosis of the oral disease from the department of medical examination so as to understand the fundamentals of pathology.
- 2021.06.
- 2021.04.
- 2021.04.
- 2021.04.
- 2020.06.
- 2020.04.
- 2020.04.
- 2019.06.
- 2019.05.
- 2019.04.
- 2018.06.
- 2018.05.
- 2018.04.
- 2017.06.
- 2017.05.
- 2017.04.
- 2016.05.
- 2016.02.
- 2016.04.
- 2015.05.
- 2015.04.
Social


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