Hiroyuki Ijima | Last modified date:2023.09.27 |
Professor /
Molecular and Biochemical Systems Engineering
Department of Chemical Engineering
Faculty of Engineering
Department of Chemical Engineering
Faculty of Engineering
Graduate School
Undergraduate School
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Homepage
https://kyushu-u.elsevierpure.com/en/persons/hiroyuki-ijima
Reseacher Profiling Tool Kyushu University Pure
http://www.chem-eng.kyushu-u.ac.jp/lab8/
Phone
092-802-2748
Fax
092-802-2748
Academic Degree
Doctor of Engineering
Country of degree conferring institution (Overseas)
No
Field of Specialization
Biochemical Engineering, Medical Engineering, Bioreactor, Biomaterial, Liver Tissue Engineering
Total Priod of education and research career in the foreign country
01years00months
Research
Research Interests
Membership in Academic Society
- Development of hybrid-type artificial liver support system
keyword : Hybrid-type artificial liver, Hepatocytes organoid, Hepatic failure rat model, Liver regeneration, High density culture, Bioreactor
1990.07We developed a novel bioreactor and application system to patients. The bioreactor realized an induction of self organoid fomation and optimization of microenvironment for cell functions by using chemical engineering protocols. Furthermore, we succesfully developed hepatic failure animal model for estimating the performance of the artificial liver system.. - Development of functional substratum and system for animal cell culture
keyword : Animal cell culture, Culture substratum, Useful biological material, Bioreactor, Hydroxyl apatite, RGD, High density culture, Mass culture
1997.04Macroporous hydroxyapatite culture substratum and packed-bed type bioreactor were developed. We suceeded mass production of colony stimulating factor using this system. Now, we focused optimization of this system and improvement of geometry, porosity and strength of the substratum.. - Development of hybrid-type artificial kidney
keyword : Hybrid-type artificial kidney, Renal proximal tubule cell, MDR, Active transport, RGD
2002.05~2006.03We focused to develop a hybrid artificial kidney with renal proximal tubule cells immobilized on adhesive synthetic RGD peptide. Immobilized cells express the selective active transport of drugs in vitro culture using transwell. Furthermore, RGD-immobilized hollow fiber module was developed. Extracorporeal circulation system equipped hepotocyte-immobilized module and renal proximal tubule cells immobilized module was developed. Blood purification effect of severe hepatic failure rat induced by acetaminophen can be expressed by using this system.. - Development of tissue-engineered liver
keyword : Liver tissue engineering, Regenerative medicine, Angiogenesis, Scaffold, Cytokine, Hepatocytes organoid, Functional biomaterials
2002.05. - Development of a cell function simulator
keyword : Drug metabolism simylator, Hepatocytes organoid, cell function, bioreactor,
2004.04. - Development of high density mass production procedd of ES cell-derived functional cells
keyword : ES cell, High density culture, mass production, bioreactor, culture system, maintenance of undifferentiated condition, differentiation, functional cell
2004.11~2021.03. - Practical research using functional biomaterials
keyword : Nanogel emulsion, nanofiber non-woven fabric, decellularized organ, preservation technology, Pancreas clip
2015.04We developed a novel bioreactor and application system to patients. The bioreactor realized an induction of self organoid fomation and optimization of microenvironment for cell functions by using chemical engineering protocols. Furthermore, we succesfully developed hepatic failure animal model for estimating the performance of the artificial liver system..
Books
Papers
Presentations
1. | Drug delivery system with nanogel emulsion applicable to cancer and central nervous system diseases . |
2. | Application development of decellularized tissue. |
3. | Uehara Uyeda Mario Kokichi、Wu Fanqi、Fukuda Yukako、Sakai Yusuke、Shirakigawa Nana、Miyata Tatsunori、Nakao Yosuke、Yamao Takanobu、Aishima Shinichi、Yamashita Yo-ichi、Baba Hideo、Ijima Hiroyuki, Development and Functional Evaluation of a Miniature Liver Model (ミニチュア肝臓モデルの開発と機能評価), 化学工学会第87年会, 2022.03. |
4. | Construction of Whole Organ Engineering, and Regenerative Medicine - Liver -, [URL]. |
5. | Nana Shirakigawa, Hiroki Sakamoto, Cho Jaeyong, Daisuke Imai, Yo-ichi Yamashita, Ken Shirabe, Yoshihiko Maehara, Hiroyuki Ijima, Fundamental technology for the creation of whole liver engineering, and functional evaluation of recellularized liver, 2015 4th TERMIS World Congress (Tissue Engineering and Regenerative Medicine International Society), 2015.09, Technology for regenerative medicine based on tissue engineering is desired earnestly as an effective medical treatment for serious organ diseases. Especially, liver is a central organ for metabolism in our body and is complicated structure. Therefore, liver tissue engineering is one of the most important and difficult themes. However, formation of tissue-like structure with the thickness more than 1mm is still impossible, because oxygen consumption rate of hepatocytes is higher than the other organs’ cells. Scale-up and easy process development are required. For the realization, creation of whole liver engineering (WLE) consisting of cells, functional ECM and fine organ template will be indispensable. Heparin-collagen conjugate and solubilized liver ECM were developed as growth factor-immobilizable materials. VEGF and HGF were immobilized on these functional materials (>90%). Hepatocytes on these materials well expressed various liver-specific functions in vitro. Hepatocytes or fetal liver cells (FLCs)-embedded functional gel was subcutaneously transplanted into rat. Angiogenesis and viability of hepatocytes were enhanced in the gel. Furthermore, transplanted FLCs form liver tissue-like structure with vascular network. Organ-scale scaffold having a template of blood vessel network was obtained by decellularization with detergent. The fineness of the network was the same as original liver, evaluated by 3D-CT. Furthermore, endothelialization and expression of liver-specific function of hepatocytes were confirmed. Furthermore, recellularized liver well metabolize ammonia during blood circulation. Based on the above-mentioned results, we expected that fundamental technology for the creation of WLE was developed. Keywords: Whole organ engineering, Decellularized liver, Liver tissue engineering, Functional ECM. |
6. | Hiroyuki Ijima, Shintaro Nakamura, Jingia Ye, Nana Shirakigawa, Daisuke Imai, Yo-ichi Yamashita, Ken Shirabe, Yoshihiko Maehara, Fundamental technology for the creation of Whole Liver Engineering, TERMIS-AP 2014 (Tissue Engineering and Regenerative Medicine International Society, Asia-Pacific Annual Conference 2014), 2014.09, Technology for regenerative medicine based on tissue engineering is desired earnestly as an effective medical treatment for serious organ diseases. Especially, liver is a central organ for metabolism in our body and is complicated structure. Therefore, liver tissue engineering is one of the most important and difficult themes. However, formation of tissue-like structure with the thickness more than 1mm is still impossible, because oxygen consumption rate of hepatocytes is higher than the other organs’ cells. Development for upsizing and easy process is required. For the realization, creation of whole liver engineering (WLE) consisting of cells, functional ECM and fine organ template will be indispensable. Heparin-collagen conjugate and solubilized liver ECM were developed as growth factor-immobilizable materials. VEGF and HGF were immobilized on these functional materials (>90%). Hepatocytes on these materials well expressed various liver-specific functions in vitro. Hepatocytes or fetal liver cells (FLCs)-embedded functional gel was subcutaneously transplanted into rat. Angiogenesis and viability of hepatocytes were enhanced in the gel. Furthermore, transplanted FLCs form liver tissue-like structure with vascular network. Organ-scale scaffold having a template of blood vessel network was obtained by decellularization with detergent. The fineness of the network was the same as original liver, evaluated by 3D-CT. Furthermore, endothelialization and expression of liver-specific function of hepatocytes were confirmed. In other words, initial structure of WLE was successfully developed. Additionally, blood circulation system containing recellularized liver and functional evaluation system of the liver were developed. Based on the above-mentioned results, fundamental technology for the creation of WLE was developed. Keywords: Whole organ engineering, Decellularized liver, Liver tissue engineering, Functional ECM. |
7. | Basic Study for Liver Tissue Engineering by Using Decellularized Organ. |
8. | Cell-embedded functional gel-filled scaffold culture for liver tissue engineering Hiroyuki Ijima, Nana Shirakigawa, Yung-Te Hou, Shintaro Nakamura, Takayuki Takei, Koei Kawakami . |
9. | Organoid formation and the function expression of primary rat hepatocytes are improved by culturing with hepatocyte growth factor-immobilized culture substratum. |
- The Society of Chemical Engineers, Japan
- Japanese Society for Artificial Organs
- The Society for Biotechnology, Japan
- The Japanese Society for Regenerative Medicine
- The Japanese Society for Biomaterials
- Japan Bioindustry Association
- Japanese Society for Alternative to Animal Experiments
- Japanese Association for Animal Cell Technology
- Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering
(JBB Volume 116, Issue 6, December 2013, Pages 740-745)
Educational
Educational Activities
Biomaterials engineering
Bichemical Engineering
Bioprocess Engineering II
Bioprocess Engineering I
The second basics physical chemistry and practice
Fundamentals of bioengineering
The second/the third materials science engineering expriment
Cell Biology
Other Educational Activities
Bichemical Engineering
Bioprocess Engineering II
Bioprocess Engineering I
The second basics physical chemistry and practice
Fundamentals of bioengineering
The second/the third materials science engineering expriment
Cell Biology
- 2008.12.
Social
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