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Yoshiki Katayama Last modified date:2021.06.11

Professor / Functional Biochemistry
Department of Applied Chemistry
Faculty of Engineering

Graduate School
Undergraduate School
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Administration Post
Dean of the Graduate School of Systems Life Sciences
Director of the Innovation Center for Medical Redox Navigation

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Introduction of Katayama Laboratory (publication list, member introduction, breaf introduction of each research) .
Academic Degree
Doctor of Engineering
Country of degree conferring institution (Overseas)
Field of Specialization
Analytical Chemistry, Biotechnology
Total Priod of education and research career in the foreign country
Research Interests
  • development of new fluorescent probes for flow cytometry with ultra-high sensitivity.
    keyword : Intracellular Signal Transduction, Immune system, stem cell, flow cytometry, FACS, fluorescence probe
  • development of molecular systems for regulation of immune responses
    keyword : Intracellular Signal Transduction, Immune system, macrophage, T cell
  • development of various molecular probing system for in vivo molecuylar imaging.
    keyword : Intracellular Signal Transduction, in vivo imaging, molecular probe, fluorescence, luminescence, MRI, protein kinase, pH, cancer diagnostics
  • New assay system for monitoring of intracellular protein kinase activity by using gold nano-particles.
    keyword : Intracellular Signal Transduction, Gold nano-particle, colorimetry, Fluorescence Analysis, diagnostics, drug discovery
  • Functionalizes MR Contrast Agent Recognizing Vascular Lesions
    keyword : Imaging of Blood Vessel, Atherosclerosis, Cardiovascular Diseases, MRI, Magnetic Resonance, Nano Particles, Organic Dyes
  • Intracellular Signal Responsive Biomaterials
    keyword : Gene Therapy, Gene Delivery, Drug Delivery, DDS
  • Comprehensive Analytical Method of Intracellular Signals
    keyword : Intracellular Signal Transduction, Surface Plasmon Resonance, Mass Spectrometry, Fluorescence Analysis, Array, Posttranslational Modification
Academic Activities
1. C. W. Kim, R. Toita, J.-H. Kang, T. Mori, A. Kishimura, Y. Katayama*, Protein kinase C α-responsive gene carrier for cancer-specific transgene expression and cancer therapy, ACS Biomater. Sci. Eng., 10.1021/acsbiomaterials.1c00213, 2021.04.
2. Y. Mu, Y. Kinashi, J. Li, T. Yoshikawa, A. Kishimura, M. Tanaka, T. Matsui, T. Mori*, K. Hase*, Y. Katayama*, Polyvinyl butyrate nanoparticles as butyrate donors for colitis treatment, ACS Applied Bio Mater.,, 4, 2335-2341, 2021.02.
3. K. Sasaki, K. Muguruma, R. Osawa, A. Fukuda, A. Taniguchi, A. Kishimura, Y. Hayashi*, T. Mori*, Y. Katayama*, Synthesis and biological evaluation of a monocyclic Fc-binding antibody-recruiting molecule for cancer immunotherapy, RSC Med. Chem.,, 12, 406-409, 2021.02.
4. 1. K. Sasaki, M. Harada, T. Yoshikawa, H. Tagawa, Y. Harada, Y. Yonemitsu, T. Ryujin, A. Kishimura, T. Mori*, Y. Katayama*, Fc-binding antibody-recruiting molecules targeting prostate-specific membrane antigen: defucosylation of antibody for efficacy improvement, ChemBioChem,, 22, 496-500, 2021.02.
5. 14. Y. Mu, Y. Kinashi, A. Kishimura, T. Mori*, K. Hase*, Y. Katayama*, Effect of polyvinyl butyrate nanoparticles incorporated with immune suppressing vitamins on alteration of population of intestinal immune cells, Prog. Nat. Sci. Mater. Int.,, 30, 707-710, 2020.10.
6. X. Sun, R. Tokunaga, Y. Nagai, R. Miyahara, A. Kishimura, S. Kawakami, Y. Katayama*, T. Mori*, Ligand design for specific MHC class I molecules on the cell surface, Biochemistry,, 59, 4646-4653, 2020.12.
7. Hiroshi Tagawa, Katsuya Maruyama, Koichi Sasaki, Natsuki Konoue, Akihiro Kishimura, Motomu Kanai, Takeshi Mori, Kounosuke Oisaki, Yoshiki Katayama, Induction of ADCC by a folic acid-mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells, RSC Advances, 10.1039/d0ra03291c, 10, 28, 16727-16731, 2020.04, We developed conjugates between monoclonal antibody (mAb) and folic acid (FA) by using a tryptophan (Trp)-selective reaction, which yields relatively homogenous products compared to conventional methods. The obtained mAb-FA conjugates showed significant cellular cytotoxicity toward folate receptor-expressing cancer cells, demonstrating that the conjugates retained the Fc region's original function..
8. Koichi Sasaki, Minori Harada, Yoshiki Miyashita, Hiroshi Tagawa, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Fc-binding antibody-recruiting molecules exploit endogenous antibodies for anti-tumor immune responses, Chemical Science, 10.1039/d0sc00017e, 11, 12, 3208-3214, 2020.03, Redirecting endogenous antibodies in the bloodstream to tumor cells using synthetic molecules is a promising approach to trigger anti-tumor immune responses. However, current molecular designs only enable the use of a small fraction of endogenous antibodies, limiting the therapeutic potential. Here, we report Fc-binding antibody-recruiting molecules (Fc-ARMs) as the first example addressing this issue. Fc-ARMs are composed of an Fc-binding peptide and a targeting ligand, enabling the exploitation of endogenous antibodies through constant affinity to the Fc region of antibodies, whose sequence is conserved in contrast to the Fab region. We show that Fc-ARM targeting folate receptor-α (FR-α) redirects a clinically used antibody mixture to FR-α+ cancer cells, resulting in cancer cell lysis by natural killer cells in vitro. Fc-ARMs successfully interacted with antibodies in vivo and accumulated in tumors. Furthermore, Fc-ARMs recruited antibodies to suppress tumor growth in a mouse model. Thus, Fc-ARMs have the potential to be a novel class of cancer immunotherapeutic agents..
9. Takanobu Nobori, Masumi Kawamura, Ryosuke Yoshida, Taisei Joichi, Kenta Kamino, Akihiro Kishimura, Eishi Baba, Takeshi Mori, Yoshiki Katayama, Fluorescence Signal Amplification by Using β-Galactosidase for Flow Cytometry; Advantages of an Endogenous Activity-Free Enzyme, Analytical chemistry, 10.1021/acs.analchem.9b04471, 92, 4, 3069-3076, 2020.02, We previously proposed using a hydrolysis enzyme for fluorescent signal amplification in flow cytometric detection of antigen proteins, which was named the catalyzed reporter penetration (CARP) method. In this method, antigen proteins are labeled with enzyme-modified antibodies, and then fluorophore-modified substrates stain cells by penetrating the cell membrane upon hydrolysis of the substrate. We proved the concept by using alkaline phosphatase (AP) as the hydrolysis enzyme. However, a required prior inactivation process of endogenous AP activity on the cell surface risked disrupting recognition of antigen proteins by antibodies. In this report, the CARP method was extended to β-galactosidase (β-gal) as an amplification enzyme, which circumvented the requirement of an initial inactivation process because endogenous β-gal activity on the surface of examined cells was found to be negligible. The substrate structure for β-gal was optimized and used for the CARP method. The CARP method showed significantly higher fluorescent signals than a conventional method using fluorophore-modified antibodies. Moreover, the degree of amplification of the fluorescence signal was higher for antigens with low expression levels, showing that the CARP method is a suitable signal amplification method over current conventional approaches..
10. #K. Zai, M. Hirota, T. Yamada, N. Ishihara, T. Mori, A. Kishimura, K. Suzuki, K. Hase, Y. Katayama, Therapeutic effect of vitamin D3-containing nanostructured lipid carriers on inflammatory bowel disease, J. Controlled Release,, 286, 94-102, 2018.09.
11. K. Sasaki, Y. Miyashita, D. Asai, D. Funamoto, K. Sato, Y. Yamaguchi, Y. Mishima, T. Iino, S. Takaishi, J. Nagano, A. Kishimura, T. Mori, Y. Katayama, A Peptide Inhibitor of Antibody-Dependent Cell-Mediated Cytotoxicity against EGFR/Folate Receptor-α Double Positive Cells, Med. Chem. Commun., 10.1039/C8MD00010G, 9, 783-788, 2018.05.
12. T. Nobori, K. Tosaka, A. Kawamura, T. Joichi, K. Kamino, A. Kishimura, E. Baba, T. Mori, Y. Katayama, Alkaline Phosphatase-Catalyzed Amplification of a Fluores-cence Signal for Flow Cytometry, Langmuir, 10.1021/acs.analchem.7b03893, 90, 2, 1059-1062, 2018.01.
13. E. Nakahei, K. Takehara, H. Sato, EK. Zai, A. Kishimura, T. Mori, Y. Katayama, Ligand design for cancer imaging with long blood circulation and enhanced accumulation ability in tumors, Med. Chem. Commun, 10.1039/C8MD00010G, 9, 783-788, 2018.02.
14. E. Nakahei, K. Takehara, H. Sato, K. Zai, A. Kishimura, T. Mori, Y. Katayama, A Dual Alkylated Peptide-ligand Enhances Affinity to Human Serum Albumin, Anal. Sci, 10.2116/analsci.17P614, 31, 4, 501-504, 2018.04.
15. Elnaz Nakhaei, Chan Woo Kim, Daiki Funamoto, Hikari Sato, Yuta Nakamura, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Ligand design for cancer imaging with long blood circulation and enhanced accumulation ability in tumors, Med. Chem. Comm., DOI: 10.1039/c7md00102a, 2017.04.
16. Cui Cui Li, Masafumi Takeo, Masayoshi Matsuda, Hiroko Nagai, Sun Xizheng, Wataru Hatanaka, Hiroyuki Inoue, Kenzaburo Tani, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Fabrication of Dendrimer‐Based Polyion Complex Submicrometer‐Scaled Structures with Enhanced Stability under Physiological Conditions, MedChemComm, DOI: 10.1039/C7MD00188F, 2017.04.
1. Yoshiki Katayama, Synthetic Molecules for regulating Antibody-dependent Cellular Cytotoxicity ~Synthetic Alternative of Therapeutic Antibody~, China-Japan Bippolymer Symposium, 2019.11.
2. Yoshiki Katayama, Chemical Systems for Immuno-Modulation, Mirai Materials Science Workshop, 2019.05.
3. 片山 佳樹, Synthetic molecules for regulating antibody-dependent cellular cytotoxicity, 2018GCBM, 2018.12.
4. 片山 佳樹, New nano-structured lipid carrier systems for autoimmune disease therapeutics, ChinaNanomedicine2018, 2018.10.
5. Yoshiki Katayama, New Nano-particular Systems for Autoimmune Inflammatory DiseaseTherapeutics, Medicinal Chemistry & Drug Design, 2018.07.
6. Yoshiki Katayama, Synthetic molecules for regulating antibody-dependent cellular cytotoxicity (ADCC), Medicinal Chemistry & Drug Design, 2018.07.
7. Yoshiki Katayama, Catalyzed Reporter Penetration:A Novel Method for Signal Amplification in Flow Cytometry, Biomaterials International 2018, 2018.07.
8. Yoshiki Katayama, Synthetic Molecules for RegulatingAntibody Dependent Cellular Cytotoxicity(ADCC), A3 Symposium, 2018.02.
9. Yoshiki Katayama, Synthetic molecules for regulating antibody-dependent cellular cytotoxicity (ADCC), A3 Foresight Forum, 2018.02.
10. Yoshiki Katayama, Catalyzed Reporter Penetration: A Nobel Method for Signal Amplification in Flow Cytometry, 1st Minisymposium on Material Biology, 2017.10.
11. Yoshiki Katayama, Synthesis of new molecular probe and nanoparticles, 2017.04.
12. Yoshiki Katayama, Cancer Targeting Systems with Signal Engineering Strategy, Inernational Symposium of Polymer Therapeutics Focusing the EPR Effect and Its Future Development, 2017.04.
13. Yujiro Hamada, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Design of nano-structured PICs using all-hydrophilic block copolymers and their site-specific incorporation of functional nanomaterials, IPC2016, 2016.12.
14. Yiwei Liu, Hengmin Tang, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Enhanced Protein Encapsulation by Polyion Complex Vesicle Induction on Protein-Polyion Complex Particle, IPC2016, 2016.12.
15. Kazuki Yuzuriha, Takeo Masafumi, Md Hosain Zahangir, Khadijah, Ayaka Yoshida, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Development of phosphatidylserine containing nanoparticle suppression inflammation, IPC2016, 2016.12.
16. Takuma Yoshikawa, Yu Tahara, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, EPR Domino: Tumor-selective vasodilation for improving EPR effect by a nitric-oxide-releasing liposome, IPC2016, 2016.12.
17. Takanobu Nobori, Shujiro Shiosaki, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Fluorophore labeled polymer conjugates for ratiometric detection of protein kinase activity, IPC2016, 2016.12.
18. Hikari Sato, Yuta Nakamura, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Improvement of Blood Circulation Time of Liposome by Coating with Serum Albumin via Ligand, ChinaNanoMedicine2016, 2016.10.
19. Takanobu Nobori, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Ratiometric real-time measurement of protein kinase activity with fluorophore labeled polyion complexes, ICONAN2016, 2016.09.
20. Hikari Sato, Yuta Nakamura, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama, Development of a Long-circulating Liposomal Carrier Coated with serum albumin, ICONAN2016, 2016.09.
21. Yoshiki Katayama, Novel analytical systems to evaluate cellular condition for cancer diagnosis, CYCU seminor, 2016.11.
22. Yoshiki Katayama, Biomaterials for modulating cellular functions with cellular-signal engineering, Biomaterials International 2016, 2016.11.
23. Yoshiki Katayama, Molecular systems to modulate immune responses in inflammatory disease or cancers, IUMRS-ICA2016, 2016.10.
24. Yoshiki Katayama, Tumor specific gene delivery system searching and regulating tumor micro- and intracellular-environment, ChinaNanoMedicine2016, 2016.10.
25. Yoshiki Katayama, Takuma Yoshikawa, Akihiro Kishimura, Takeshi Mori, Enhancement of EPR effect using nitric oxside releasing liposome, Controlled Release Society Annual Meeting & Excposition, 2016.07.
26. Yoshiki Katayama, Intracellular Protein Kinase C-alpha Responsive Gene Carriers for Malignant Tumor Cell Specific Therapy, 10th World Biomaterial Congress (WBC2016), 2016.05.
27. Yoshiki Katayama, Molecular Systems for Disease Cell-Specific Gene Regulation with Peptide-Grafted Polymers, 日本化学会第96回春季年会, 2016.03.
28. Yoshiki Katayama, Synthetic Alternative of Therapeutic Antibody, The 8th Takeda Science Foundation Symposium on PharmaSciences, 2016.01.
29. Yoshiki Katayama, Protein Kinase-alpha Responsive Molecular System for Tumor Imaging and Therapy, 第74回日本癌学会学術総会, 2015.10, これまでのがん治療では不可能な、悪性度の高いがん細胞に有効で、かつ可視化もできる全く新しい概念として、がんのPKCαシグナルを用いる分子システムの創製に関して招待講演した。その後、共同研究の申し込みが寄せられている。.
30. Yoshiki Katayama, How we can access to Cancers? Development of Cancer Treatment Strategies Using Signal Engineering, The 1st Annual International Symposium on Bio-Therapeutics Delivery , 2015.09, 細胞内シグナルを利用する新しい遺伝子治療概念と、それをがんに対して適用し、従来法にはない特異性と効果、副作用の抑制が可能であることを招待講演した。.
31. Yoshiki Katayama, Purnima Kumar, Md. Zahanzir Hosain, Daiki Funamoto, Akihiro Kishimura, Takeshi Mori, Suppression of Inflammatory Cytokines with "Eat-me" Signal Bearing Particles, 42nd Controlled Release Society Annual Meeting & Exposition, 2015.07, 炎症におけるマクロファージの表現型を抗炎症型に転換できる新しい材料と、それによる治療効果について講演した。.
32. Yoshiki Katayama, Yuta Nakamura, Kai LI, Daiki Funamoto, Akihiro Kishimura, Takeshi Mori, Gene Delivery System Using Serum Proteins for Effective Cancer Therapy, Biomedical International 2015, 2015.06, 血清タンパクを用いる新しいナノメディスンの安定化法について招待講演を行った。.
33. Yoshiki Katayama, Small Molecule that Exerts a Similar Activity to Antibody Drug, JSPS A3 Foresight International Symposium on Nano-Biomaterials and Regenerative Medicine, 2014.10.
34. Yoshiki Katayama, Design of Highly Cancer Cell Specific Gene Delivery System using Intracellular Signal-Responsive Gene Regulation Cerrier, The 3rd International Symposium og Materials on Regenerative Medicine, 2014.08.
35. Yoshiki Katayama, Design of Molecular Systems for Modulation of Immune System, 2014 CYCU Biomaterials Summer Workshop, 2014.08.
36. Yoshiki Katayama, Intracellular Signal-Responsive Carriers as New Strategy to Secure Cell Specificity in Tumor Gene Delivery, Controlled Release Society Annual Meeting and Exposition, 2014.07, 細胞内シグナルを利用する極めて疾患細胞特異性の高い遺伝子送達概念の創製について講演した。.
37. Yoshiki Katayama, Intracellular signal-responsive gene regulation delivery to address recent problem in cancer targeting, A3 Foresight Symposium, 2013.12.
38. Yoshiki Katayama, Cell signal-responsive gene regulation delivery for tumor-specific therapy and imaging, International Symposium on Theranostics Nanomolecules (Fall Meeting, Polymer Societyof Korea), 2013.10, がん細胞で特異的に亢進している細胞内の酵素に応答して遺伝子を開放できる新しい分子システムを用いた遺伝子治療とバイオイメージングの概念に関して、その実際と展開を講演した。.
39. Yoshiki Katayama, Cell signal-responsive gene delivery for tumor-specific theranostics, 2nd International Symposium on Nanomedicine Molecular Science, 2013.10.
40. 片山 佳樹, INtracellular signal-responsive gene regulation delivery to address recent problem in cancer targeting, 2013 Grobal Innovation Research Center Symposium, 2013.07.
  • Molecular Systems for Disease Cell-Specific Gene Regulation with Peptide-grafted Polymers