Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Takahisa Miyamoto Last modified date:2021.07.05

Professor / Division of Food Science & Biotechnology / Department of Bioscience and Biotechnology / Faculty of Agriculture


Papers
1. Yu Zhang, Hung-Hsin Huang, Hoang Minh Duc, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Endolysin LysSTG2: Characterization and application to control Salmonella Typhimurium biofilm alone and in combination with slightly acidic hypochlorous water, Food Microbiology, 10.1016/j.fm.2021.103791, 98, 103791, 2021.09.
2. Cunkuan Shen, Chikako Machida, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Effect of Selected Food Additives on Biofilm Formation by Foodborne Pathogens on Stainless Steel, 九州大学大学院農学研究院紀要, 10.5109/4363550, 66, 1, 45-52, 2021.04.
3. TAKASHI TSUGUKUNI, NAOFUMI SHIGEMUNE, MOTOKAZU NAKAYAMA, AND TAKAHISA MIYAMOTO, Morphological Changes in Spores during Germination in Bacillus cereus and Bacillus subtilis, Biocontrol Science, 10.4265/bio.25.203, 25, 4, 203-213, 2020.12.
4. Yu Zhang, Kumiko Shigemura, Hoang Minh Duc, Cunkuan Shen, Hung-Hsin Huang, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Effects of bacteriophage on inhibition and removal of mixed biofilm of enterohemorrhagic Escherichia coli O157:H7 and O91:H-, LWT - Food Science and Technology, 10.1016/j.lwt.2020.109945, 134, 109945, 2020.12.
5. Salako, D.A., Trang, P.N., Ha, N.C., Miyamoto, T. and Ngoc, T.T.A., Prevalence of antibiotic resistance Escherichia coli isolated from Pangasius catfish (Pangasius hypophthalmus) fillet during freezing process at two factories in Mekong Delta Vietnam, Food Research, 10.26656/fr.2017.4(5).160 , 4, 5, 1785-1793, 2020.10.
6. Apisada Kitichalermkiat, Mao Katsuki, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Effect of epigallocatechin gallate on gene expression of Staphylococcus aureus, Journal of Global Antimicrobial Resistance , 10.1016/j.jgar.2020.06.006, 22, 854-859, 2020.09.
7. Yoshimitsu Masuda, Erika Sakamoto, Ken-ichi Honjoh, Takahisa Miyamoto, Role of Toxin-Antitoxin-Regulated Persister Population and Indole in Bacterial Heat Tolerance, Applied and Environmental Microbiology, 10.1128/AEM.00935-20, 86, 16, e00935-20, 2020.08.
8. Cunkuan Shen, Md Tariqul Islam, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Transcriptional changes involved in inhibition of biofilm formation by ε-polylysine in Salmonella Typhimurium, Applied Microbiology and Biotechnology (2020) 104:5427–5436, https://doi.org/10.1007/s00253-020-10575-2, 104: 5427–5436, 2020.04.
9. Hoang Minh Duc, Hoang Minh Son, Pham Hong Ngan, Jun Sato, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Isolation and application of bacteriophages alone or in combination with nisin against planktonic and biofilm cells of Staphylococcus aureus, Applied Microbiology and Biotechnology, https://doi.org/10.1007/s00253-020-10581-4, 104, 5145-5158, 2020.04.
10. J. Sato, M. Nakayama, A. Tomita, T. Sonoda, T. Miyamoto, Difference in the antibacterial action of epigallocatechin gallate and theaflavin 3,3’-di-O-gallate on Bacillus coagulans, Journal of Applied Microbiology, doi:10.1111/jam.14662, 2020.04.
11. Hoang Minh Duc, Hoang Minh Son, Hazel Pang Shu Yi, Jun Sato, Pham Hong Ngan, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157:H7 in different food matrices, Food Research International, https://doi.org/10.1016/j.foodres.2020.108977, 131, 108977, 2020.01.
12. Mahmoud Ge. Zayda, Yoshimitsu Masuda, Ahmed M. Hammad, Ken-ichi Honjoh, Abdelrahman M. Elbagory, Takahisa Miyamoto, Molecular characterization of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese, International Dairy Journal, https://doi.org/10.1016/j.idairyj.2020.104646, 104, 104646, 2020.02, In this study, we investigated the characteristics of S. aureus isolates causing bovine subclinical mastitis (SCM), and their genetic relatedness with the isolates obtained from Egyptian cheese. Twenty-five S. aureus isolates were identified from 150 SCM milk and 75 cheese samples. The antibiogram revealed multidrug-resistant (MDR) isolates. Fifteen isolates were categorized as MRSA. Antimicrobial resistance and virulence genes were detected. Spa typing and SCCmec classification were performed. More than 50% of isolates were found carrying human-specific virulence determinants, while other isolates characterized were presumed to be of bovine-origin. Spa-types t084, t688, t304, corresponded isolates from SCM and cheese; the similar genotypes from SCM and cheese displayed divergence in virulence traits. Our results revealed the novel spa-type ‘t18546’. Animals and dairy food could be a reservoir for transformative changes in S. aureus virulence, leading to the emergence of virulent MDR strains that may become potential public-health threats..
13. Yue Guo, Xiaowen Cui, Liushu Ou, Chika Isowaki, Yoshimitsu Masuda, Ken-ichi Honjoh and Takahisa Miyamoto, Effects of Sucrose on Heat Resistance and Gene Expression in Salmonella Typhimurium, Food Science and Technology Research, 10.3136/fstr.25.903, 25, 6, 903-913, 2019.11.
14. Aye Thida Maung, Tahir Noor Mohammadi, Satoko Nakashima, Pei Liu, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto, Antimicrobial resistance profiles of Listeria monocytogenes isolated from chicken meat in Fukuoka, Japan, International Journal of Food Microbiology, doi.org/10.1016/j.ijfoodmicro.2019.05.016, 304, 49-57, 2019.09, In this study, the antimicrobial resistance profiles of L. monocytogenes isolated from chicken meat in Fukuoka in
2017 were compared with the isolates of 2012. A total of 85 and 50 chicken meat samples, including different
body parts, were collected from different supermarkets in Fukuoka in 2012 and 2017, respectively. Detection,
isolation, identification, and characterization of L. monocytogenes were performed according to the conventional
methods. Forty-five among 85 samples (53%) were positive for L. monocytogenes in 2012, while 12 among 50
samples in 2017 (24%) tested positive. One hundred fifty-three and 29 L. monocytogenes strains were isolated in
2012 and 2017, respectively. The serotypes of isolates in 2012 were 1/2a (21.5%), 1/2b (73.9%), 1/2c (1.5%),
and 4b/4e (3.1%). In contrast, the 2017 isolates showed 1/2a (48.3%) and 1/2b (51.7%) serotypes. While all
isolates in 2012 were positive for hlyA (listeriolysin O) in the PCR assay with hlyA primer set 7, only 17 hlyA
positive isolates were seen in 2017. Moreover, 75 isolates with different ribotypes in 2012 and 29 isolates in
2017, respectively, were tested for antimicrobial susceptibility by broth microdilution for 18 different antimicrobial
agents. Most of the 2012 and 2017 isolates displayed antimicrobial susceptibility. However, among the
2012 and 2017 isolates, 98.7% and 100% of the isolates were resistant to cefoxitin, 57.3% and 95.7% to fosfomycin,
72.0% and 82.6% to oxacillin, 8.0% and 17.4% to clindamycin, respectively. In addition, 2.7% of the
isolates in 2012 were resistant to flomoxef and 4.3% of the isolates in 2017 to linezolid. Multidrug resistance
(MDR) to 3 or more antimicrobials was observed in 35/75 (46.7%) isolates of 2012 and 19/23 (82.6%) in 2017.
Detection of antimicrobial resistance (AMR) genes by PCR showed that the resistant isolates of 2012 were
positive for mecA (96.3%) and ermC (83.3%), whereas the resistant isolates in 2017 screened positive for mecA
(94.7%) and mefA (25.0%). Other cfxA, ermA, ermB, fosA, fosB, and fosC genes were absent in the PCR assay for
any of the isolates. This study investigated for the first time the change in the L. monocytogenes contamination of
chicken meat and antibiotic resistance of the isolated L. monocytogenes strains in Fukuoka, Japan, in the course of
5 years. Although the contamination rate of L. monocytogenes in 2017 was found to be lower than that in 2012,
AMR of the isolates in 2017 was higher..
15. Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Hoang Minh Duc, Motokazu Nakayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken Ichi Honjoh, Takahisa Miyamoto, Baicalein inhibits Stx1 and 2 of EHEC Effects of baicalein on the cytotoxicity, production, and secretion of shiga toxins of enterohaemorrhagic escherichia coli, Toxins, 10.3390/toxins11090505, 11, 9, 505-505, 2019.08.
16. Xiaowen Cui, Chuanqi Hu, Liushu Ou, Yumiko Kuramitsu, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Transcriptional analysis on heat resistance and recovery from thermal damage in Salmonella under high salt condition, LWT-Food Science and Technology, 10.1016/j.lwt.2019.02.056, 106, 194-200, 2019.06, Sodium chloride maintains osmotic pressure of living cells including bacteria. Heat treatment is common for decontamination of bacteria in food. In this study, effects of NaCl on heat resistance of Salmonella Typhimurium were investigated. After cultivation in TSB containing 0.5% (TSB), 4% (4SC) and 8% (8SC) NaCl, S. Typhimurium cells were heated at 60 °C for 20 min. Total viable counts including intact cells and injured but recoverable cells determined by the plating method using TSA of S. Typhimurium cultured in 4SC and 8SC were significantly higher than those of the cells cultured in TSB. Meanwhile, changes of gene transcription were analyzed by DNA microarray. Transcription of genes involved in the colanic acid synthesis largely increased after cultivation in 4SC and 8SC. The amount of colanic acid significantly increased in the cells cultured in 4SC compared to that in M9-glucose medium. After recovery culture for 3 h, the genes involved in the phage shock response strongly up-regulated, suggesting contribution of these gene products in recovery of heat injured cells. The outcome of this study contributes to understand the mechanism of cross protection in Salmonella..
17. Akihito Fujimoto, Keisuke Ito, Noriko Narushima, Takahisa Miyamoto, Identification of lactic acid bacteria and yeasts, and characterization of food components of sourdoughs used in Japanese bakeries, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2018.10.014, 127, 5, 575-581, 2019.05, Sourdough is a low-pH, fermented product prepared using lactic acid bacteria and yeast mixed with rye flour, wheat flour, and water. It is used and backslopped in bakeries because it enhances texture, flavor, and dough expansion of bread. Various lactic acid bacteria and yeasts have been identified in sourdough, especially in the West. However, microbial and physical characteristics of sourdough from Japan have not been investigated. Here, we characterized the microbial composition and food component characteristics of sourdough from four bakeries in Kansai region, Japan, and performed sensory and quality evaluation of baguettes enriched with 10% sourdough. We detected different species of lactic acid bacteria such as Lactobacillus brevis, Lactobacillus alimentarius, Lactobacillus pentosus, Lactobacillus vaccinostercus, Lactobacillus sanfranciscensis, and Lactobacillus sakei. The identified yeasts primarily included Saccharomyces cerevisiae, with Candida humilis detected in some samples. Components such as amino acids, lactic acid, acetic acid, ethanol, 3-methyl-1-butanol, ethyl acetate, and phenethyl alcohol differed among samples and distinctively affected flavor, quality, and aroma of sourdough-enriched baguettes. The different species of lactic acid bacteria and the ratio of lactic acid bacteria to yeasts possibly affected food components such as free amino acids, sugars, and organic acids via the Maillard reaction, which influences the savory aromas of bread. Future investigation of the effect of lactic acid bacteria will help to improve the overall quality of bread..
18. T. Noor Mohammadi, A. T. Maung, J. Sato, T. Sonoda, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Mechanism for antibacterial action of epigallocatechin gallate and theaflavin-3,3′-digallate on Clostridium perfringens, Journal of Applied Microbiology, 10.1111/jam.14134, 126, 2, 633-640, 2019.02, Aim: The purpose of this study was to clarify the mechanism of the antibacterial action of two high potential and natural food additives, epigallocatechin gallate (EGCg) and theaflavin-3,3′-digallate (TF3), on Clostridium perfringens. Methods and Results: Minimal inhibitory concentrations were determined by the serial dilution method. Afterwards, the cells were treated with 250 or 1000 mg l
−1
of EGCg and 125 or 500 mg l
−1
of TF3 and morphological changes were observed and cell sizes were also measured under fluorescence microscopy. Our results showed that TF3 had a twice stronger antibacterial activity than EGCg against C. perfringens. Phase-contrast and fluorescence microscopy confirmed that the bacterial cells elongated without DNA segregation and septum formation in the presence of 250 mg l
−1
EGCg. While in the higher concentration of EGCg and TF3, cell growth was suppressed. Bacterial cells reached to around 12 μm after the 24 h incubation with 250 mg l
−1
EGCg, but the cells were shorter than the control at 1000 mg l
−1
of EGCg. After washing and incubating the elongated cells in fresh medium, DNA segregated at 2 h of incubation. The average cell length decreased gradually and reached the normal size at 8 h. Conclusion: It seems that EGCg at a low concentration affected the proteins involved in the septum formation, DNA segregation and cell division. Furthermore, the high concentration of EGCg and TF3 seemed to cause stronger cellular damage to C. perfringens. Significance and Impact of the Study: These polyphenols are widely distributed in all higher plants especially in tea plants, and people tend to use natural food additives rather than synthetic ones. EGCg and TF3, as natural food additives, can prevent C. perfringens food poisoning along with other potential health benefits..
19. Apisada Kitichalermkiat, Masahiro Kurahachi, Ai Nonaka, Motokazu Nakayama, Kanami Shimatani, Naofumi Shigemune, Takashi Tsugukuni, Jun Hitomi, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken-ichi Honjoh1 and Takahisa Miyamoto, Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus, Food Science and Technological Research, doi: 10.3136/fstr.25.277, 25, 2, 277-285, 2019.02, This study investigated the effect of epigallocatechin gallate (EGCg) on Staphylococcus aureus to
determine its mechanism of antibacterial action. Adsorption of EGCg on the cell envelope of S. aureus after
EGCg treatment was demonstrated using a FITC-labeled antibody specific to EGCg. After EGCg treatment
of S. aureus for 4 h, abnormalities in septum formation and cell segregation were observed at concentrations
greater than 250 mg/L, and debris presumed to arise from cell destruction or leakage of cytoplasmic materials
was observed around the cells at 500 mg/L. Two-dimensional electrophoresis of proteins prepared from
EGCg-treated S. aureus cells revealed the presence of 18 protein spots that disappeared or showed markedly
decreased intensity compared to those from control cells. These proteins included DnaK, elongation factor
G, DNA-directed RNA polymerase, l-lactate dehydrogenase, pyruvate dehydrogenase, and acetate kinase.
Furthermore, S. aureus showed decreased glucose uptake after EGCg treatment. These results suggest that
EGCg inhibits the functions of cell-envelope proteins, and it causes cellular damage and disruption of the
cells in S. aureus..
20. Ayumi Musou-Yahada, Ken-Ichi Honjoh, Kenta Yamamoto, Takahisa Miyamoto, Hideaki Ohta, Utilization of single nucleotide polymorphism-based allele-specific PCR to Identify shiikuwasha (citrus depressa hayata) and calamondin (Citrus madurensis Lour.) in processed juice, Food Science and Technology Research, 10.3136/fstr.25.19, 25, 1, 19-27, 2019.01, To develop a method for the identification of shiikuwasha (Citrus depressa Hayata) and calamondin (Citrus madurensis Lour.), trnL-trnF and trnT-trnL intergenic spacer regions of their chloroplast DNA were amplified using PCR and the nucleotide sequences were determined. In each region, a single nucleotide polymorphism (SNP) site specific to the respective citrus species (shiikuwasha and calamondin) was found. For species discrimination using PCR, two forward primers containing the allele-specific SNP site at the 3’-end and a mismatched nucleotide at the 3
rd
base from the 3’-end were designed. The allele-specific forward primers specific to shiikuwasha and calamondin were respectively designated CiDeLF-F and CiMaTL-F. To confirm the specificity of the designed primers, PCR was carried out with DNA prepared from citrus peel or hand-squeezed juice as the template. Results showed that shiikuwasha and calamondin fruits and juices were identifiable by PCR using the allele-specific primers. Furthermore, this allele-specific PCR method can be applied to industrially processed and concentrated juice by amplifying DNA in advance..
21. Hoang Minh Son, Hoang Minh Duc, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Application of bacteriophages in simultaneously controlling Escherichia coli O157:H7 and extended-spectrum beta-lactamase producing Escherichia coli, Applied Microbiology and Biotechnology, 10.1007/s00253-018-9399-1, 102, 23, 10259-10271, 2018.12, Shiga toxin-producing Escherichia coli (STEC) O157:H7 and extended-spectrum beta-lactamase (ESBL) producing E. coli (ESBLEC) are important bacteria of public health concern and frequently isolated from raw beef products. Bacteriophage-based methods have been increasingly exploited to control bacterial contamination in meats. Here, we describe the isolation, characterization, and application of a lytic phage PE37 for the simultaneous bio-control of STEC O157:H7 and ESBLEC. Phage PE37, isolated from the bovine intestine, was morphologically characterized as a member of the Myoviridae family, with a broad host range and great stability under various stress conditions. Sequencing analysis revealed that the genomic DNA of phage PE37 contains genes that contribute to virion structure, replication, assembly, and host lysis. PE37 significantly reduced the viable counts of STEC O157:H7 by 4.9 and 2.6 log CFU/mL in broth after 6 h of incubation at 25 and 8 °C, respectively. Application of phage PE37 to raw beef artificially contaminated with STEC O157:H7 resulted in significant reductions in the viable counts by 2.3 and 0.9 log CFU/piece after 24 h of storage at 25 and 8 °C, respectively. Treatment of raw beef contaminated with a bacterial cocktail of STEC O157:H7 and ESBLEC with PE37 also significantly decreased the viable counts of the bacterial mixture by 1.4 and 1.0 log CFU/piece after 24 h of incubation at 25 and 8 °C, respectively. These findings suggest that bacteriophage PE37 may be a potential bio-agent for controlling STEC O157:H7 and ESBLEC contamination in raw beef..
22. Ken–ichi HONJOH*, Hitomi OKANO1, Aya KAWABATA1, Masaru KUROKAWA1, Taiki KIMURA1, Takeshi MACHIDA2, Yoshimitsu MASUDA and Takahisa MIYAMOTO, Freezing Tolerance of Lactuca sativa and Induction of CBF and GolS Genes during Cold Treatment, J. Fac. Agr., Kyushu Univ, 63, 2, 249-257, 2018.09, Plants respond to several environmental changes such as low– and high–temperatures, drought, flood,
and so on, leading to development of stress tolerance. Acquisition abilities of freezing tolerance of three
lettuce cultivars were investigated. All the investigated cultivars showed improvement of freezing tolerance
at –3°C after exposure to non–freezing low–temperature at 2°C for 7 days. Out of them, papa lettuce cultivar
was used to investigate expression levels of cold–responsive genes. The cultivar showed induction of
CBF and GolS genes, which are well known as cold–responsive genes in other plants, during cold treatment.
These results showed that lettuce has at least one cold–responsive signal pathway, suggesting a possibility
that genetical modification might enhance the freezing tolerance..
23. Xiao–guang ZHANG1,2,*, Sachiko TSUJI2, Hayato KITAOKA2, Mitsuru TAMAI2, Hiroshi KOBAYASHI3, Ken–ichi HONJOH4 and Takahisa MIYAMOTO4, Preparation and Characterization of Monoclonal Antibodies Suitable for Detection of Foodborne Pathogens by Biosensor, J. Fac. Agr., Kyushu Univ., 63, 2, 319-330, 2018.09, Monoclonal antibodies (MAbs) for detection of Escherichia coli O157:H7 (O157:H7), Salmonella
Enteritidis (SE) and Listeria monocytogenes (LM) using surface plasmon resonance (SPR) biosensor
were prepared and characterized. Indirect enzyme–linked immunosorbent assay (ELISA) and SPR biosensor
were used for screening of the hybridoma cells secreting MAbs specific to the pathogens. Based on the
reactivity of MAbs against the target pathogens by SPR biosensor, MAbs were selected. For O157:H7, the
clones 3–11B–3F–8 and 3–11B–3F–11, which culture supernatants reacted strongly with boiled O157:H7
and sonicated O157:H7 cells were obtained and their culture supernatants were used for purification of
anti–O157:H7 MAb. For SE, the clone 1–11G–8, which generated high response to sonicated SE cells and
the lowest response to the sonicated mixture cells was obtained and the culture supernatant was used for
purification of anti–SE MAb for detection of sonicated SE cells. The clone of 3–5H–3F was found with high
reactivity against boiled SE and very low against the other boiled samples and was selected for purification
of anti–SE MAb for detection of boiled SE cells. For LM, the clone of 2F6–7 that reacted strongest with
boiled LM 4b was obtained and the culture supernatant was used for purification of anti–LM 4b MAb. The
clone of 13H9–2 was found to generate almost greatest response against sonicated LM 1/2a and low response
to sonicated L. innocua. Moreover, this MAb reacted strongly with boiled LM 4b without cross–reactivity
against the other bacteria. This clone was selected for purification of anti–LM MAb for the detection of sonicated
LM 1/2a or LM 4b cells and boiled LM 4b cells. Although MAbs for LM showed cross–reactivity
against L. innocua, the MAbs obtained after screening by the combined method showed a capacity to detect
target pathogens by using SPR biosensor as well as ELISA. These MAbs are useful for detection of pathogens
by biosensor and are expected to contribute to the development of rapid detection of the pathogens..
24. Hoang Minh Duc, Hoang Minh Son, Ken-ichi Honjoh, Takahisa Miyamoto, Isolation and application of bacteriophages to reduce Salmonella contamination in raw chicken meat, LWT - Food Science and Technology, https://doi.org/10.1016/j.lwt.2018.01.072, 91, 353-360, 2018.05, Chicken meats are considered as main sources associated with Salmonella infections in humans. In this study,
lytic phages against Salmonella were isolated and examined for their efficacy to control Salmonella. Eighteen lytic
phages were isolated from raw chicken skin and gizzard. Five phages belonging to Myoviridae and Siphoviridae
families were characterized and selected for bacterial challenge tests. The treatment of raw chicken breast
samples contaminated with S. Enteritidis and S. Typhimurium at 8 °C by the cocktail of five phages significantly
reduced (P < 0.05) viable counts by 1.41 and 1.86 log CFU/piece, respectively. When incubated at 25 °C, the
highest reductions of viable counts of S. Enteritidis and S. Typhimurium in the phage-treated samples were 3.06
and 2.21 log CFU/piece, respectively (P < 0.05). These data suggested that the phages isolated from raw
chicken meats are potential agents for controlling Salmonella in raw meats..
25. XIAOWEN CUI, HSU-MING SHERMAN WEN, YOSHIMASA KINOSHITA, SHOTA KOISHI, CHIKA ISOWAKI, LIUSHU OU, YOSHIMITSU MASUDA, KEN-ICHI HONJOH, TAKAHISA MIYAMOTO, Role of Phage Shock Protein in Recovery of Heat-injured Salmonella, Biocontrol Science, https://doi.org/10.4265/bio.23.17, 23, 1, 17-25, 2018.03.
26. Akihito Fujimoto, Keisuke Ito, Madoka Itou, Noriko Narushima, Takayuki Ito, Akihisa Yamamoto, Satoru Hirayama, Soichi Furukawa, Yasushi Morinaga, and Takahisa Miyamoto, Microbial behavior and changes in food constituents during fermentation of Japanese sourdoughs with different rye and wheat starting materials, Journal of Bioscience and Bioengineering , 125, 1, 97-104, 2018.01, Sourdough is a food item made by kneading grain flour and water together and allowing fermentation through the
action of lactic acid bacteria (Lactobacillales) and yeast. Typically, Japanese bakeries make sourdough with rye flour,
wheat flour, malt extract, and water and allow spontaneous fermentation for 6 days. We compared the microbial
behavior and food components, such as organic acids, sugars, and free amino acids, of sourdoughs made using two
different rye and wheat flours during the 6-day fermentation period. Comparisons were made for two types of rye and
wheat flours, using different production sites and different milling, distribution, and storage conditions. The microbial
count was evaluated using different culture media. All sourdough types showed a significant increase in lactic acid levels
on fermentation day 2 and a decrease in free amino acid levels on day 4. Low overall lactic acid production and little
fluctuation in sugar levels occurred in sourdough made from French ingredients. For sourdough made from Japanese
ingredients, sugar levels (chiefly glucose, sucrose, and maltose) declined on fermentation day 1, increased on day 2, and
declined by day 5. With the French ingredients, no yeast cells were detected until day 3, and many acid precursors of
sourdough flavor components were detected. Yet with the Japanese ingredients, 106/g yeast cells were detected on days
3e5, as well as sourdough-flavor esters and alcohols. Differences in raw material quality affected the microbial behavior
and changes in food constituents during the fermentation process and, consequently, the sourdough flavor.
27. Furuta M, Nasu T, Umeki K, Hoang Minh D, Honjoh K, Miyamoto T, Characterization and Application of Lytic Bacteriophages against Campylobacter jejuni Isolated from Poultry in Japan, Biocontrol Science, 22, 4, 218-221, 2017.12.
28. Jun Sato, Motokazu Nakayama, Ayumi Tomita, Takumi Sonoda, Motomitsu Hasumi, Takahisa Miyamoto, Evaluation of repetitive-PCR and matrixassisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid strain typing of Bacillus coagulans, PLOS one, https://doi.org/10.1371/journal.pone.0186327, 12, 10, e0186327, 2017.11, In order to establish rapid and accurate typing method for Bacillus coagulans strains which
is important for controlling in some canned foods and tea-based beverages manufacturing
because of the high-heat resistance of the spores and high tolerance of the vegetative cells
to catechins and chemicals, matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS) and repetitive-PCR (rep-PCR) were evaluated. For this purpose,
28 strains of B. coagulans obtained from various culture collections were tested. DNA
sequence analyses of the genes encoding 16S rRNA and DNA gyrase classified the test
strains into two and three groups, respectively, regardless of their phenotypes. Both MALDITOF
MS and rep-PCR methods classified the test strains in great detail. Strains classified in
each group showed similar phenotypes, such as carbohydrate utilization determined using
API 50CH. In particular, the respective two pairs of strains which showed the same metabolic
characteristic were classified into the same group by both MALDI-TOF MS and rep-
PCR methods separating from the other strains. On the other hand, the other strains which
have the different profiles of carbohydrate utilization were separated into different groups by
these methods. These results suggested that the combination of MALDI-TOF MS and rep-
PCR analyses was advantageous for the rapid and detailed typing of bacterial strains in
respect to both phenotype and genotype..
29. Xiaoguang Zhang, Sachiko Tsuji, Hayato Kitaoka, Hiroshi Kobayashi, Mitsuru Tamai, Ken-Ichi Honjoh, Takahisa Miyamoto, Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor, Journal of Food Science, 10.1111/1750-3841.13843, 82, 10, 2357-2363, 2017.10.
30. Takahisa Miyamoto, Xiaoguang Zhangb,, Development of novel monoclonal antibodies directed against catechins for investigation of antibacterial mechanism of catechins, Journal of Microbiological Methods, http://dx.doi.org/10.1016/j.mimet.2017.03.014, 137, 6-13, 2017.03, Catechins are major polyphenolic compounds of green tea. To investigate mechanism for antibacterial action of
catechins, 11 monoclonal antibodies (MAbs) were raised against a 3-succinyl-epicatechin (EC)-keyhole limpet
hemocyanin (KLH) conjugate. Amino acid sequences of variable regions determined for MAbs b-1058, b-1565,
and b-2106 confirmed their innovative character. MAb b-1058 strongly interacted with its target substances in
the following order of magnitude: theaflavin-3,3′-di-O-gallate (TFDG) > theaflavin-3-O-gallate
(TF3G) ≥theaflavin-3′-O-gallate (TF3’G) > gallocatechin gallate (GCg) > penta-O-galloyl-β-D-glucose
(PGG) > epigallocatechin gallate (EGCg), as determined using surface plasmon resonance (SPR) on MAbimmobilized
sensor chips. The affinity profiles of MAbs b-1058 and b-2106 to the various polyphenols tested
suggested that flavan skeletons with both carbonyl oxygen and hydroxyl groups are important for this
interaction to take place. S. aureus cells treated with EGCg showed green fluorescence around the cells after
incubation with FITC-labeled MAb b-1058. The fluorescence intensity increased with increasing concentrations
of EGCg. These MAbs are effective to investigate antibacterial mechanism of catechins and theaflavins..
31. Md Tariqul Islam, Aya Ogura, Chikako Machida, Noriko Morinaga, Ken-ichi Honjoh, Takahisa Miyamoto, Effects of ε-polylysine and Milk Serum Protein on the Attachment and Decontamination of Salmonella Enteritidis on Lettuce and Radish Sprouts, Food Science and Technology Research, 10.3136/fstr.22.703, 22, 5, 703-711, 2016.10.
32. FUYUKI AOYAMA, Takahisa Miyamoto, Development of a DNA Array for the Simple Identification of Major Filamentous Fungi in the Beverage Manufacturing, Biocontrol Science, 21, 3, 161-172, 2016.06, Filamentous fungi were isolated from the indoor environment of a soft drink manufacturing
plant and ordinary residences. The isolated strains were identified based on morphological
observation and the nucleotide sequences of the region near the D2 region of the 26S rDNA.
Three genera (Aspergillus, Penicillium, and Cladosporium) accounted for 48.1% of the fungal
strains detected in the manufacturing plant and 75.3% in residences. A DNA array for identification
of 15 genera and 26 species of filamentous fungi that were most frequently isolated from the
manufacturing plant was developed. Genus- and species-specific probes with 13- to 20-mer were
designed on the basis of the nucleotide sequences in the D2 region. The probes were affixed to
a microscope slide after modifying an amino group at the 5’or 3’end. To prevent erroneous identification,
2 or 3 probes were designed for each of the target genera and species. The developed
DNA array method correctly identified 9 genera (Alternaria, Aureobasidium, Cladosporium,
Curvularia, Exophiala, Fusarium, Penicillium, Phoma, and Trichoderma) and 26 species belonging
to 6 genera (Aspergillus, Neosartorya, Byssochlamys, Talaromyces, Paecilomyces, and
Purpureocillium) in the strains isolated from the indoor environment. Identification results
obtained by this DNA array method of fungi isolated from the manufacturing plant were consistent
with those by the conventional method..
33. Rui Li a, Xiao Tan, Jie Xiao, Hongxun Wang, Zhiguo Liu, Min Zhou , Wanglai Bi, Takahisa Miyamoto, Molecular screening and characterization of Shiga toxin-producing Escherichia coli in retail foods, Food Control, http://dx.doi.org/10.1016/j.foodcont.2015.07.045, 60, 180-188, 2016.06, Shiga toxin-producing Escherichia coli (STEC) strains are one of the most important recently emerged
groups of food borne pathogens. This study investigated the prevalence of molecular markers for STEC
and characteristics of E. coli O157 isolates from foods sold at retail markets in Wuhan, China. A total of
489 samples (350 meat products and 139 raw vegetables) were purchased from 22 large scale markets
between July of 2011 and September of 2013. The meat samples consisted of frozen chicken products,
raw pork, raw beef, frozen fish products and processed duck products. The raw vegetable samples
consisted of lettuce, bok choy, radish, spinach, cucumber, and tomato. Shiga toxin genes (stx1 and stx2)
and an O-group marker of the seven main pathogenic STEC serogroups (O157, O26, O45, O103, O111,
O121, and O145) were detected in the samples by using PCR. 100% agreement was obtained between the
results of the PCR targeting for wzyO157 and the PCR targeting for rfbEO157 gene. The result demonstrated
that PCR assay targeting for wzyO157 gene can be employed as an effective screening method for E. coli
O157 in food sample. In the study, E. coli O157 and non-O157 STEC were detected in 55 (11.2%) and 75
(15.3%) samples by PCR screening, respectively. There was significant difference in the occurrence of STEC
contamination between supermarkets (19/127, 15.0%) and open markets (111/362, 30.7%) (P < 0.05). Out
of 489 samples, 5 samples carried O45, 1 sample carried O145 and 1 sample carried O111. Markers for
O103, O26 and O121 were not detected. This result differed from other reports. Immunomagnetic separation
based cultivation technique was used to isolate E. coli O157 from 27 food samples collected in
2013. Finally 7 E. coli O157 isolates were obtained. Among the 7 isolates, the prevalent stx genotype was
stx1a and stx2a. Four E. coli O157 strains exhibited toxic effects on Vero cells, while 3 isolates had no
detectable cytotoxicity effects even though they contained stx genes. All E. coli O157 isolates were sensitive
to the 12 antimicrobials tested except for roxithromycin. There are some inconsistencies between
the PCR screening and culture results. Characteristics of STEC isolates should be evaluated and considered
for monitoring STEC contamination in foods..
34. Ken-ichi Honjoh, Yuri Iwaizako, Yin Lin, Nobuyuki Kijima, Takahisa Miyamoto, Possibilities for Contamination of Tomato Fruit by Listeria monocytogenes during Cultivation, Food Science and Technology Research, 10.3136/fstr.22.349, 22, 3, 349-357, 2016.05.
35. Hoang Minh Duc, Hoang Minh Son, Ken-ichi Honjoh, Takahisa Miyamoto, Isolation and bio-control of Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli contamination in raw chicken meat by using lytic bacteriophages., LWT - Food Science and Technology, 10.1016/j.lwt.2016.04.013, 71, 339-346, 2016.04.
36. Hoang Minh Son, Hoang Minh Duc, Honjoh, K., Takahisa Miyamoto, Identification of the newly identified subtilase cytotoxin-encoding gene (subAB2-2) among clinical Shiga toxin-producing Escherichia coli isolates, Canadian Journal of Microbiology, 10.1139/cjm-2015-0519, 61, 12, 990-994, 2015.10, Subtilase cytotoxin (SubAB) is an important virulence factor of eae-negative Shiga toxin-producing
Escherichia coli (STEC). Three variants of SubAB-encoding genes have been reported in the literature; however, the
newly described subAB variant (subAB2-2).
37. Fuyuki Aoyama, Takahisa Miyamoto, Development of polymerase chain reaction and multiplex polymerase chain reaction for simple identification of thermoanaerobic spore-forming bacteria, Food Science and Technology Research, 10.3136/fstr.21.531, 21, 4, 531-536, 2015.08, Thermoanaerobic spore-forming bacteria such as Thermoanaerobacter, Moorella, Thermoanaerobacterium, and Caldanaerobius produce spores with extremely high resistance to heat and are known to spoil various sealed, sterile drinks, in particular, low-acid drinks distributing at high temperature, such as canned coffee containing milk. These bacteria are hard to handle and to identify on the basis of traditional biochemical characteristics. We have developed novel primers for single and multiplex PCR method for rapid and simple identification of these bacteria at the genus level. They were correctly identified approximately 2 h after DNA extraction among 86 strains of 35 species of gram-positive and –negative bacteria including various spore-forming bacilli. Furthermore, new LAMP primers have been designed to develop a specific detection method for T. mathranii and T. thermocopriae, the most problematic microbes in food industries, because of their extremely high resistance against heat and various antibacterial agents. Our LAMP method using the novel primers was easy to detect these microbes. Our present methods effectively improve the complicated procedures in quality control of raw materials and products in food industry..
38. Motokazu Nakayama, Daisuke Tomiyama, Naofumi Shigemune, Asako Mitani, Wenjie Xu and Takahisa Miyamoto, Cell surface Hydrophobicity Contributes to Lactobacillus Tolerance to Antibacterial Actions of Catechins, Food Science and Technology Research, doi: 10.3136/fstr.21.583, 21, 4, 583-588, 2015.08, Although most Gram-positive bacteria are sensitive to epigallocatechin gallate (EGCg), some species of lactic
acid bacteria (LAB) are highly tolerant. The mechanism of LAB tolerance to the antibacterial action of EGCg
was investigated. LAB strains with three different cell wall composition types were used: Lactobacillus plantarum
NBRC15891 (meso-DAP-type), Lactobacillus fermentum NBRC15885 (Orn-type), and Lactobacillus delbrueckii
NBRC3073 (Lys-type). The minimum inhibitory concentration of EGCg for L. plantarum NBRC15891, L. fermentum
NBRC15885, and L. delbrueckii NBRC3073 were >1000, >1000, and 500 μg/mL at pH 6.5, respectively. The cell
surface hydrophobicity (CSH), and contents of extracellular polymeric substances (EPS) and teichoic acid of these
strains suggested that strains with low CSH and producing greater amounts of EPS are highly resistant to EGCg at
pH 6.5. After EGCg treatment, the membrane potential decreased in strains with high susceptibility to EGCg. Our
findings suggested that LAB characterized by high EPS level and low CSH are resistant to EGCg at pH 6.5..
39. Motokazu NAKAYAMA, Daisuke TOMIYAMA, Keisuke IKEDA, Mao KATSUKI, Ai NONAKA, Takahisa Miyamoto, Antibacterial Effects of Monoglycerol Fatty Acid Esters and Sucrose Fatty Acid Esters on Bacillus spp., Food Science and Technology Research, 10.3136/fstr.21.431, 21, 3, 431-437, 2015.06, Fatty acid esters are food additives with strong antibacterial activity against spore forming bacteria. The antibacterial activity of monoglycerol fatty acid esters (MG) and sucrose fatty acid esters (SE) with various fatty acid chain lengths was systematically investigated on four typical Bacillus species: B. cereus, B. subtilis, B. megateirum and B. coagulans. Monoglycerol monolaurate (MG12) and monoglycerol monomyristate (MG14) showed relatively strong bactericidal effects on vegetative cells of these spesies among the MGs tested at both pH6.0 and pH8.0. Different SEs showed bactericidal effects on different species at pH6.0, while they had no antibacterial effects on the three species except for B. coagulans at pH8.0. SE showed antibacterial effects on vegetative cells of these species as is the case of MG. In addition, it was found that MG and SE showed strong antibacterial effects on both B. cereus and B. subtilis in logarithmic phase of growth, and antibacterial activity of MG continued longer than that of SE..
40. Motokazu nakayama, Kanami Shimatani, Tadahiro Ozawa, Naofumi Shigemune, Daisuke Tomiyama, Koji Yui, Mao Katsuk, Keisuke Ikeda, Ai Nonaka, Takahisa Miyamoto, Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis,, Bioscience, Biotechnology, and Biochemistry, 10.1080/09168451.2014.993356, 79, 5, 845-854, 2015.05, Catechins are class of polyphenols and have high anti-bacterial activity against various microorganisms. Here we report the mechanism for antibacterial activity of EGCg against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis..
41. Son Hoang Minh, Etsuko Kimura, Duc Hoang Minh, Ken-ichi Honjoh, Takahisa Miyamoto, Virulence characteristics of Shiga toxin-producing Escherichia coli from raw meats and clinical samples, Microbiology and Immunology, 10.1111/1348-0421.12235., 59, 3, 114-122, 2015.03.
42. Md Tariqul Islam, Akinobu Oishi, Chikako Machida, Aya Ogura, Shoken Kin, Ken-ichi Honjoh, Takahisa Miyamoto, Combined effects of selected food additives on adhesion of various foodborne pathogens onto microtiter plate and cabbage leaves , Food Control, 10.1016/j.foodcont.2014.05.034, 46, 233-241, 2014.12.
43. Ken-ichi Honjoh, Tomoko Mishima, Nozomi Kido, Misako Shimamoto, Takahisa Miyamoto, Investigation of possible contamination routes of Salmonella via soils and the effects of mulch for avoiding its contamination to leafy lettuce plants during cultivation, Food Science and Technology Research, 20, 5, 961-969, 2014.09.
44. Takahisa Miyamoto, Seiyo Toyofuyku, Motokzu Nakayama, Ken-ichi Honjoh, Specific inhibition of cytotoxicity of Shiga-like toxin 1 of enterohemorrhagic Escherichia coli by gallocatechin gallate and epigallocatechin gallate, Food Control, http://dx.doi.org/10.1016/j.foodcont.2014.02.017, 42, 263-269, 2014.08.
45. Xiaoguang Zhang, Takahisa Miyamoto, Ken-ichi Honjoh, Development of a Simultaneous Detection Method for Foodborne Pathogens Using Surface Plasmon Resonance Biosensors, Food Science and Technology Research, 10.3136/fstr.20.317, 20, 2, 317-325, 2014.03.
46. Toshihiko Fukuda, Takahisa Miyamoto, Tanaka Mitsuru, Toshiro Matsui, Attenuation of L-Type Ca2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats, PLoS ONE, 10.1371/journal.pone.0088975, 9, 2, e88975, 2014.02.
47. Xiaoguang Zhang, Sachiko Tsuji, 本城 賢一, 宮本 敬久, Effects of Pretreatments on Detection of E. coli O157:H7 by SPR Biosensor, Journal of the Faculty of Agriculture, Kyushu University ,  Vol. 58, , No. 2 , 307-312, 2013.09.
48. Motokazu Nakayama, Kanami Shimatani, Tadahiro Ozawa, Naofumi Shigemune, Takashi Tsugukuni, Daisuke Tomiyama, Masahiro Kurahachi, Ai Nonaka, Takahisa Miyamoto, A study of the antibacterial mechanism of catechins: Isolation and identification of Escherichia coli cell surface proteins that interact with epigallocatechin gallate, Food Control, 10.1016/j.foodcont.2013.03.016, 33, 2, 433-439, 2013.03.
49. Miyamoto, T., Mekada, Y., Kurahachi, M., Umeno, M., Nakayama, M., Shigemune, N., Tsugukuni, T, Tokuda, H., Tachibana, H., Honjoh, K., A highly sensitive method for quantifying gallocatechin gallate and its epimer using a catechin-specific peptide , Food Control , 29, 1, 162-166, 29(1), 162-166(2013.01), 2013.01.
50. Mishima, T., Kido, N., Nakashima, S., Yamakawa, M., Miyaji, N., Soli, K.W., Honjoh, K., Md. Bari, L. and Miyamoto, T., Investigation of possible situation of internalization of salmonella enteritidis in tomato fruits and bacterial survival during tomato plant cultivation, Food Sci. Technol. Res., 18, 6, 869-877, 18(6):869-877(2012.11), 2012.11.
51. Machida, T., Ishibashi, A., Kirino, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Chloroplast NADPH-Dependent Thioredoxin Reductase from Chlorella vulgaris Alleviates Environmental Stresses in Yeast Together with 2-Cys Peroxiredoxin, PLoS ONE, 10.1371, 7, 9, e45988 , 7(9),e45988 (2012.09), 2012.09.
52. Eui-Baek Byun, Takahisa Miyamoto, Toshiro Matsui, A procyanidintrimer,C1,promotesNOproductioninrataorticendothelial cells via both hyperpolarizationandPI3K/Aktpathways, EuropeanJournalofPharmacology, http://dx.doi.org/10.1016/j.ejphar.2012.07.011, 692, 52-60, 2012.07.
53. Nakayama, M., Shigemune, N., Tsugukuni, T., Hitomi, J., Matsushita, T., Mekada, Y., Kurahachi, M., Miyamoto, T., Mechanism of the combinedanti-bacterial effect of green tea extract and NaCl against Staphylococcus aureus and Escherichia coli O157:H7, Food Control, 25, 1, 225-232, 25(1):225-232(2012.05), 2012.05.
54. Naofumi Shigemune, Motokazu Nakayama, Takashi Tsugukuni, Jun Hitomi, Chihiro Yoshizawa, Yoko Mekada, Masahiro Kurahachi, Takahisa Miyamoto, The mechanisms and effect of epigallocatechin gallate (EGCg) on the germination and proliferation of bacterial spores, Food Control, 10.1016/j.foodcont.2012.04.003, 27, 2, 269-274, 2012.04.
55. Liu, P., Mizue, H., Fujihara, K., Kobayashi, H., Kamikado, H., Tanaka, T., Honjoh, K. and Miyamoto, T., A New Rapid Real-Time PCR Method for Detection of Listeria monocytogenes Targeting the hlyA Gene, Food Sci. Technol. Res., 18, 1, 47-57, 18(1):47-57(2012.01), 2012.01.
56. Soli, K.W., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K. and Miyamoto, T., Application of a Combined Decontamination Method for Fresh Produce Using SAHW, Sucrose Fatty Acid Ester and Microbubbles, Food Sci. Technol. Res., 17, 6, 555-559, 17(6):555-559(2011.11), 2011.11.
57. Miyamoto T, Mishima T, Kido N, Nakashima S, Honjoh K, Salmonella contamination of leaf lettuce cultivated on a soil inoculated with the bacterium, The 5th International Symposium on the East Asian Environmental Problems, FUKUOKA GARDEN PALACE(福岡市), 2011.11.
58. Li R, Zhai P, Dai S, Miyamoto T, Shiga-toxin producing E. coli contamination of commercially available raw meat and fresh vegetables in central china, The 5th International Symposium on the East Asian Environmental Problems, FUKUOKA GARDEN PALACE(福岡市), 2011.11.
59. Miyamoto, T., Kawagishi, J., Oishi, A., Shimotsu, S., Mishima, T., Kobayashi, H., Honjoh, K., Inhibition of adhesion of several bacteria onto microtiter plate by selected food additives, Jpn. J. Food Microbiol., 28, 3, 157-166, 28(3):157-166, 2011.10.
60. Nakayama, M., Shigemune, N., Tsugukuni, T., Tokuda, H., Miyamoto, T., Difference of EGCgadhesion on cell surface between Staphylococcus aureus and Escherichia coli visualized by electron microscopy after novel indirect staining with cerium chloride, Journal of Microbiological Methods, 86, 1, 97-103, 86(1):97-103(2011.07), 2011.07.
61. Wen, H.-M., Naito, K., Kinoshita, Y., Kobayashi, H., Honjoh, K., Tashiro, K., Miyamoto, T., Changes in transcription during recovery from heat injury in Salmonella typhimurium and effects of BCAA on recovery, Amino Acids, 10.1007/s00726-011-0934-y, 42, 6, 2059-2066, 2011.05.
62. Miyamoto, T., Ohishi, A., Kawagishi, J., Ishibashi, A., Kinoshita, Y., Mekada, Y., Honjoh, K., Effects of commercial kitchen detergents on adhesion and viability of bacteria, Nippon Shokuhin Kagaku Kogaku Kaishi, 58(3):127-130(2011.04)..
63. Li, R., Harada, T., Honjoh, K., Miyamoto, T., Phylogenetic analysis and Shiga toxin production profiling of Shiga toxin-producing/enterohemorrhagic Escherichia coli clinical isolates, Microb Pathog., 49, 5, 246-251, 49(5):246-251, 2010.11.
64. Machida, T., Honjoh, K., Aso, A., Yamamoto, M., Ho, M., Miyamoto, T. , Trehalose 6-Phosphate Synthase and Trehalose 6-Phosphate Phosphatase from Nicotiana tabacum Function in Trehalose Biosynthesis and Environmental Stress Tolerance of Yeast, Journal of the Faculty of Agriculture, Kyushu University, 55, 2, 261-268, 55(2):261-268, 2010.10.
65. Soli, K.W., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K., Miyamoto, T. , Bacterial Contamination and Microflora of Several Fresh Produce, Journal of the Faculty of Agriculture, Kyushu University, 55, 2, 269-273, 55(2):269-273, 2010.10.
66. Soli, K.W., Motomatsu, A., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K., Miyamoto, T. , Comparison of the Bactericidal Effect of Slightly Acidic Hypochlorous Water with That of Conventional Sterilizers, Journal of the Faculty of Agriculture, Kyushu University, 55, 2, 275-280, 55(2):275-280, 2010.10.
67. Nakayama, M., Shigemune, N., Tsugukuni, T., Tokuda, H., Miyamoto, T. , A simple and rapid turbidimetric method for determining catechins in beverages, International Journal of Food Science & Technology, 45, 10, 2071-2079, 45(10):2071-2079, 2010.10.
68. Trevanich, S., Tiyapongpattana, S., Miyamoto, T., Application of an optimized 18-h method involving one step culturing and single primer-based PCR assay for detection of Salmonella spp. in foods, Food Control, 21, 5, 593-598, 21(5):593-598(2010.05), 2010.05.
69. Soli, K.W., Yoshizumi, A., Motomatsu, A., Yamakawa, M., Yamasaki, M., Mishima, T., Miyaji, N., Honjoh, K., Miyamoto, T., Decontamination of fresh produce by the use of slightly acidic hypochorous water following pretreatment with sucrose fatty acid ester under microbubble generation, Food Control, 21, 9, 1240-1244, 21(9):1240-1244, 2010.04.
70. Honjoh, K., Matsuura, K., Machida, T., Nishi, K., Nakao, M., Yano, T., Miyamoto, T., Iio, M., Enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: Co-expression of cDNA clones, from Cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in Saccharomyces cerevisiae, Amino Acids, 38, 4, 1173-83, 38(4):1173-83. , 2010.04.
71. Machida, T., Ohashi, N., Mimura, A., Honjoh, K., Iio, M., Miyamoto, T. , Chloroplastic Glucose 6-Phosphate Dehydrogenase from Chlorella vulgaris Alleviates Freezing and Menadione-Induced Oxidative Stresses in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University, 55, 1, 29-38, 55(1):29-38 , 2010.02.
72. Miyamoto, T., Nakayama, M., Shigemune, N., Tokuda, H., Matsushita, T., Furuta, K., Mekada, Y., Honjoh, K. Application of green tea extract as food preservative to improve shelf life of overnight pickled cucumber, Nippon Shokuhin Kagaku Kogaku Kaishi, 56, 12, 660-664 (2009) .
73. Machida, T., Honjoh, K., Shimizu, H., Yamamoto, M., Iio, M., Miyamoto, T., Expression of a gene encoding a functional glycosyl hydrolase, trehalase, from Nicotiana tabacum in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University, 54, 2, 297-304, 2009.11.
74. Honjoh, K., Hashimoto, Y., Shimotsu, S., Wen, H.-M., Kiriki, M., Naito, K., Tokugawa, M., Satake, E., Kobayashi, H., Miyamoto, T., Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ∆agfA and ∆bcsA mutants of Salmonella Enteritidis; ∆ahpC, ∆ahpF, and ∆katG mutants of S. Typhimurium; and ∆rpoE, ∆rpoH, and ∆rpoS mutants of S. Enteritidis and S. Typhimurium, Journal of the Faculty of Agriculture, Kyushu University, 54, 2, 421-432, 2009.11.
75. Miyamoto, T., Wen, H.-M., Kinoshita, Y., Kobayashi, H., Honjoh, K., Tashiro, K., BCAA Promote Recovery from Heat-Injury in Salmonella Typhimurium, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 38th Annual meeting, 103-106, 103-106, 2009.10.
76. Kobayashi, H., Kubota, J., Fujihara, K., Honjoh, K., Iio, M., Fujiki, N., Nakabe, M., Oda, S., Takasu, K., Nakanishi, H., and Miyamoto, T. , Simultaneous enrichment of Salmonella spp, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes by single broth and screening of the pathogens by multiplex real-time PCR
, Food Science and Technology Research, 15(4): 427-438, 2009.07.
77. Fratamico, P., DebRoy, C., Miyamoto, T., Liu, Y., , PCR detection of enterohemorrhagic E.coli O145 in food by targeting genes in the E.coli O145 O-antigen gene cluster and the shiga toxin 1 and shiga toxin 2 genes, Foodborne pathogens and disease, 6: 605-611, 2009. 5., 6, 5, 605-611, 6: 605-611 (2009), 2009.05.
78. Machida, T., Kato, E., Ishibashi, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., and Miyamoto, T., Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin, Biosci. Biotechnol. Biochem.,, 73, 3, 695-701, 2009.03.
79. Combined effects of surfactants and preservatives on the antibacterial activity of green tea extracts.
80. Honjoh, K., Fujihara, K., Haraguchi, T., Ono, Y., Kobayashi, H., Hiwaki, H., Kamikado, H., Jang, S.S., Ryu, S., and Miyamoto, T., Subtyping of Listeria monocytogenes based on nucleotide polymorphism in clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates, Food Science and Technology Research, 14(6): 557-564, 2008.12.
81. Machida, T., Murase, H., Kato, E., Honjoh, K., Matsumoto, K., Miyamoto, T., and Iio, M., Isolation of cDNAs for hardening-induced genes from Chlorella vulgaris by suppression subtractive hybridization., Plant Science, 17(3): 238-246, 2008.09.
82. Machida, T., Kato, E., Ishibashi, A., Ohashi, N., Honjoh, K., and Miyamoto, T., Molecular characterization of low-temperaure-inducible NTR-C in Chlorella vulgaris, Nucleic Acids Symposium Series, 51: 463-464, 2007.11.
83. Miyamoto, T., Fujihara, K., Honjoh, K., Kamikado, H., Tanaka, T., Intraspecific lineage group identification of Listeria monocytogenes based on DNA sequences of some genes and rapid detection of L. monocytogenes genetically similar to clinical isolates, Proceedings of the UJNR 36th annual meeting, p.39-42, 2007.10.
84. Miyamoto, T. , Rapid detection methods for food pathogens, Program & Abstracts, Japan-Germany International Cooperative Project on Education and Research Multi-functionality and Sustainability of Land Use in SE. and E. Asia , p.43, 2007.09.
85. Honjoh, K., Machida, T., Nishi, K., Matsuura, K., Soli, K.W., Sakai, T., Ishikawa, H., Matsumoto, K., Miyamoto, T., and Iio, M., Improvement of freezing and oxidative stress tolerance in Saccharomyces cerevisiae by taurine, Food Science and Technology Research, 13, 2, 145-154, 13(2):145-154, 2007.05.
86. Honjoh,K., Machida, T., Hagisako, T., Suga, K., Yonekura, M., Shimizu, H., Ohashi, N., Miyamoto, T., Hatano, S., and Iio, M., Molecular cloning and characterization of a cDNA for low-temperature inducible cytosolic glucose 6-phosphate dehydrogenase gene from Chlorella vulgaris and expression of the gene in Saccharomyces cerevisiae, Plant Science, 172, 3, 649-658, 172(3):649-658, 2007.03.
87. Hiwaki, H., Ebuchi, S., Baba, A., Miyamoto, T., Genotyping of Listeria monocytogenes Strains by PFGE, RAPD-PCR, ERIC-PCR and PCR-SSCP based on the iap gene, Bokin Bobai, 35(1):13-22(2007.01)..
88. Fujimoto, A., Torii, K., Watanabe, M., Miyamoto, T., Antimicrobial Activities of Fatty Acid Esters against B. stearothermophilus Strains Isolated from Liquid Seasoning, Bokin Bobai, 34(11):693-701(2006.11)..
89. Fujimoto, A., Torii, K., Watanabe, M., Miyamoto, T., Studies on the Growth Behavior of B. stearothermophilus Strains Isolated from Liquid Seasoning, Bokin Bobai, 34(10):617-624(2006.10)..
90. Jang, S.S., Choo, E.Y., Han, K.S., Miyamoto, T., Heu, S., Ryu, S.R. , Antibiotic resistance and genetic diversity of Listeria monocytogenes isolated from chicken carcasses in Korea., Journal of Microbiology and Biotechnology, 16, 8, 1276-1284, 16(8):1276-1284 , 2006.08.
91. Hiwaki, H., Baba, A., Ebuchi, S., Uryu, K., Miyazaki, E., Miyamoto, T., Behavior of Listeria monocytogenes in the Manufacturing Process of Karashi-Mentaiko, Jpn. J. Food Microbiol., 23(2): 85-92(2006.07). .
92. Hiwaki, H., Baba, A., Ebuchi, S., Miyamoto, T., Detection of Listeria monocytogenes by real time PCR assay using Light Upon Extension Fluorogenic Primer, Bokin Bobai, 34(6):329-338(2006.06)..
93. Miyamoto, T., Murata, Y., Kobayashi, H., Shimoyae, M., Kamikado, H., Noda, N., Maruyama, K., Honjoh, K., Iio, M., Enumeration of viable counts in raw milk using the automated fluorescence microscopic method, Biocontrol Science, 10, 4, 147-154, 10(4):147-154, 2005.12.
94. Miyamoto, T., Nakayama, M., Sasaki, C., Sadakari, K., Itoh, Y., Fujimoto, A., Honjoh, K., and Iio, M., Simple identification of some Bacillus species and related genera in food by RAPD analysis combined with morphological observation., Biocontrol Science, 10, 1,2, 45-53, 10(1): 45-53, 2005.06.
95. Matsui, T., Ueno, T., Tanaka, M., Oka, H., Miyamoto, T., Osajima, K., Matsumoto, K., Antiproliferative action of an angiotensin I-converting enzyme inhibitory peptide Val-Tyr, via an L-Type Ca2+ channel inhibition in cultured vascular smooth muscle cells, Hypertens Res., 10.1291/hypres.28.545, 28, 6, 545-552, 28(6):545-552, 2005.06.
96. Kobayashi, H., Miyamoto, T., Hashimoto, Y., Kiriki, M., Motomatsu, A., Honjoh, K., and Iio, M., Identification of factors involved in recovery of heat-injured Salmonella Enteritidis., J. Food Prot., 68, 5, 932-941, 68 (5): 932-941, 2005.01.
97. Kamikado, H., Ito, A., Sugimoto, Y., Suzuki, A., Tsujimoto, Y., Endo, M., Matsunaga, E., Miyamoto, T., Iio, M. , Characterization of microflora in raw milk and powdered milk, Bokin Bobai, 32(5):243-250(2004)..
98. Kamikado, H., Ito, A., Aoki, F., Endo, M., Ohtsuka, A., Miyamoto, T. and Iio, M., Studies on the effect of sodium hypochlorite and electrolyzed oxidizing water on the viability of bacillus spores and the factors affecting the sporicidal action, Sci. Bull. Fac. Agr., Kyushu Univ., 59(1):15-24(2004)..
99. Miyamoto, T., Kamikado, H., Sasaki, C., Sadakari, S., Honjoh, K. and Iio, M., An attempt to identify Bacillus cereus by PCR., Biocontrol Science, 9, 3, 69-75, 9(3): 69-75, 2004.01.
100. Miyamoto, T., Shimizu, Y., Kobayashi, H., Honjoh, K., and Iio, M., Studies of collagen binding with immobilized Salmonella enteritidis and inhibiton with synthetic and naturally occurring food additives by a surface plasmon resonance biosensor., Sensors and Materials., 15, 8, 453-466, 15(8): 453-466, 2003.12.
101. Miyamoto, T. and Iio, M., Detection of Bacillus cereus by PCR, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 32nd Annual meeting, 2003.11.
102. Nakayama, M., Fujimoto, A., higuchi, A., Watanabe, M., Sadakari, K., Iio, M., Miyamoto, T.,:Studies on Growth Characteristics of Bacillus Strains Isolated from a Liquid Seasoning, Nippon shokuhin kagaku kougaku kaishi,50(11):537-545(2003)..
103. M. Nakayama, A. Fujimoto, A.higuchi, M. Watanabe, K. Sadakari, M. Iio, T. Miyamoto, Studies on the growth behavior of Bacillus strains isolated from liquid seasonings, Bokin-bobai, 31(9): 479-484 (2003)..
104. Nakayama, M., Fujimoto, A., higuchi, A., Watanabe, M., Sadakari, K., Iio, M., Miyamoto, T., Frequency at which Bacillus spp. were isolated from liquid seasonings and changes after storage, Bokin-bobai, 31(9): 473-478(2003)..
105. Honjoh, K., Mimura, A., Kuroiwa, E., Hagisako, T., Suga, K., Shimizu, H., Dubey, R.S., Miyamoto, T., Hatano, S., and Iio, M., Purification and characterization of two isoforms of glucose 6-phosphate dehydrogenase (G6PDH) from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.67.1888, 67, 9, 1888-1896, 67(9): 1888-1896, 2003.09.
106. Nakayama, M., Fujimoto, A., Higuchi, A., Watanabe, M., Iio, M., Miyamoto, T., The effects of slightly acidified hypochlorous acid water on the viability of various Bacillus spores and Leuconostoc sp. cells, Bokin-bobai, 31(8): 421-425 (2003)..
107. Kobayashi, H., Miyamto, T., Honjoh, K., Iio, M., Development of a rapid and simple method for measuring total viable bacterial counts by flow cytometry, Bokin-bobai, 31(7): 357-363 (2003)..
108. Miyamoto, T., Kamikado, H., Kobayashi, H., Honjoh, K., and Iio, M., Immunomagnetic flow cytometric detection of staphylococcal enterotoxin B in raw and dry milk, J. Food Protection., 66, 7, 1222-1226, 66(7):1222-1226, 2003.07.
109. Kamikado, H., Ohshima, A., Aoki, F., Sugimoto, Y., Ohnishi, K., Endo, M., Miyamoto, T., Iio, M., Influence of milk ingredients on the rapid detection of coliforms by bioluminometric assay and correlation of the rapid method with the standard method, Bokin-Bobai, 31(4): 183-190 (2003)..
110. Shimizu, H., Furuya, N., Suga, K., Honjoh, K., Miyamoto, T., Hatano, S., and Iio, M., Freezing tolerance of transgenic tobacco with increased content of unsaturated fatty acid by expressing the CvFad2 or CvFad3 gene., J. Fac. Agr., Kyushu Univ., 47, 2, 307-317, 47(2):307-317, 2003.02.
111. Honjoh, K., Suga, K., Shinohara, F., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M., Preparation of protoplasts from Chlorella vulgaris K-73122 and cell wall regeneration of protoplasts from C. vulgaris K-73122 and C-27, J. Fac. Agr., Kyushu Univ., 47, 2, 257-266, 47(2): 257-266, 2003.02.
112. Kamikado, H., Aoki, F., Tsujimoto, Y., Endo, M., Miyamoto, T., Iio, M., Rapid Detection of Coliforms in Drinking Milk by Using a Highly Sensitive Assay of β-Galactosidase Based on the Bioluminescence Reaction, Bokin Bobai,31(1):7-11(2003)..
113. Miyamoto, T., Kamikado, H., Kobayashi, H., Iio, M., Immunomagnetic-flow cytometric detection of staphylococcal enterotoxin B in raw and dry milk, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 31th Annual meeting, Monterey, California, I1-7, 2002.12.
114. Suga, K., Honjoh, K., Furuya, N., Shimizu, H., Nishi, K., Shinohara, F., Hirabaru, Y., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M.,, Two low-temperature-inducible Chlorella genes for D12 and w-3 fatty acid desaturase (FAD): Isolation of D12 and w-3 fad cDNA clones, expression of D12 fad in Saccharomyces cerevisiae, and expression of w-3 fad in Nicotiana tabacum, Biosci. Biotechnol. Biochem., 66, 6, 1314-1327, 66(6): 1314-1327, 2002.06.
115. Miyamoto, T., Sayed, Md. A., Sasahara, R., Sukimoto, K., Umezaki, A., Honjoh, K., Iio, M. and Hatano, S., Cloning and overexpression of Bacillus cereus penicillin-binding protein 3 gene in Escherichia coli, Biosci. Biotechnol. Biochem., 66, 1, 44-50, 66(1): 44-50, 2002.01.
116. Miyamoto, T., Ichioka, N., Sasaki, C., Kobayashi, H., Honjoh, K., Iio, M. and Hatano, S., Polymerase chain reaction assay for detection of Escherichia coli O157:H7 and Escherichia coli O157:H-, J. Food Protection, 65, 1, 5-11, 65(1): 5-11 (2002), 2002.01.
117. Miyamoto, T., Kobayashi, H., Iio, M., Recovery of Stressed Salmonella enteritidis, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 30th Annual meeting, Tukuba, Ibaraki., 48-51, p. 48-51, 2001.11.
118. Honjoh,K., Shimizu, H., Nagaishi, N., Matsumoto, H., Suga, K., Miyamoto, T., Iio, M., and Hatano, S., Improvement of freezing tolerance in transgenic tobacco leaves by expressing the hiC6 gene, Biosci. Biotechnol. Biochem., 10.1271/bbb.65.1796, 65, 8, 1796-1804, 65(8): 1796-1804, 2001.08.
119. Miyamoto, T., Iio, M., and Hatano, S., Freezing injury of E. coli O157:H7 and DNA region specific to E. coli O157, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 29th Annual meeting,Waikiki, Hawaii, E- 1-12 (2000)., E- 1-12, 2000.11.
120. Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Takata, R., Joh, T., Suga, K., Miyamoto, T., Iio, M. and Hatano, S., Cryoprotective activities of group3 late embryogenesis abundant proteins from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1656, 64, 8, 1656-1663, 64(8): 1656-1663, 2000.08.
121. Kim, S.-I., Miyamoto, T., Honjoh, K., Iio, M., and Hatano, S., Molecular cloning, overproduction and characterization of the Bacillus cereus IMP dehydrogenase, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1210, 64, 6, 1210-1216, 64(6): 1210-1216, 2000.06.
122. Miyamoto, T., Rapid detection of Salmonella by PCR and Using Quartz Crystal Microbalance, Jpn. J. Food Microbiol., 17(4),217-224(2000)..
123. Trevanich, S., Miyamoto, T., Harada, Y., Honjoh, K. and Hatano, S., Rapid detection of enterotoxigenic Escherichia coli O6 in water by using monoclonal antibody and a photon-counting TV camera, J. Food Protection, 63, 4, 534-538, 63(4): 534-538, 2000.04.
124. Miyamoto, T., Sukimoto, K., Sayed, Md.A., Kim, S.-I., Honjoh, K., and Hatano, S., Detection of penicillin-binding proteins in Bacillus cereus by using biotinylated β-lactams, J. Fac. Agr., Kyushu Univ., 44, 3,4, 299-307, 44(3, 4): 299-307 (2000), 2000.03.
125. Miyamoto, T., Yoshida, Y., Etoh, S., Honjoh, K., Iio, M. and Hatano S., Studies on freezing injury of unwashed and washed cells of Escerichia coli O157:H7, Jpn. J. Food Microbiology, 17(2): 127-133(2000)..
126. Miyamoto, T., Trevanich, S., Hatano, S., Application of random amplified polymorphic DNA analysis and DNA sensor using quartz crystal microbalance for rapid detection of salmonella SPP. in foods, Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, 28th Annual meeting, Tukuba, Ibaraki, 90-106, p. 90-106, 1999.12.
127. Miyamoto, T., Trevanich, S., Honjoh, K., Hatano, S., Rapid detection of Salmonella spp. by PCR amplification of Salmonella specific region in gatD gene, 日本食品微生物学会雑誌, 16, 2, 99-109, 16(2): 99-109, 1999.02.
128. Miyamoto, T., Trevanich, S., Okabe, T., Tomoda, S., Honjoh, K., Hatano, S., Rapid detection method of Salmonella-specific PCR product by DNA-immobilized quartz crystal microbalance., Jpn. J. Food Microbiol., 16(1): 57-63(1999)..
129. Honjoh, K., Oda, Y., Takata, R., Miyamoto, T. and Hatano, S., Introduction of the hiC6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast, Journal of Plant Physiology, 155, 4,5, 509-512, 155(4,5): 509-512, 1999.01.
130. Miyamoto, T., Kuramitsu, Y., Ookuma, A., Trevanich, S., Honjoh, K., Hatano, S., Rapid detection of counting of viable bacteria in vegetables and environmental water using a photon-counting TV camera, J. Food Protection, 61, 10, 1312-1316, 61(10): 1312-1316, 1998.10.
131. Miyamoto, T., Tian, H.-Z., Okabe, T., Trevanich, S., Asoh, K., Tomoda, S., Honjoh, K., Hatano, S., Application of random amplified polymorphic DNA analysis for detection of Salmonella spp. in foods., J. Food Prot., 61, 7, 785-791, 61(7): 785-791, 1998.07.
132. Takata, R., Miyamoto, Y., Honjoh, K., Soeda, T., Sakamoto, J., Miyamoto, T., Hatano, S., Antibody fragments as inhibitors of Japanese radish acid phosphatase, Biosci. Biotechnol. Biochem., 10.1271/bbb.62.1041, 62, 6, 1041-1047, 62(6): 1041-1047, 1998.06.
133. Miyamoto, T., Matsuno, K., Imamura, M., Kim, S.-I., Honjoh, K., and Hatano, S., Purification and some properties of IMP dehydrogenase of Bacillus cereus., Microbiological Research , 153, 1, 23-27, 153(1), 23-27, 1998.01.
134. Miyamoto, T., Imamura, M., Matsuno, K., Kim, S.-I., Honjoh, K., Hatano, S., Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus., Microbiol. Res., 152, 3, 277-280, 152(3):277-280, 1997.12.
135. Miyamoto, T., Yamaguchi, K., Md A. Sayed, Sasahara, R., Honjoh, K., Hatano, S., Penicillin-binding protein sensitive to cephalexin in sporulation of Bacillus cereus., Microbiol. Res., 152, 3, 227-232, 152(3):227-232, 1997.12.
136. Kawata, S., Tanaka, Y., Ohto, T., Miyamoto, T., Hatano, S., Detection method of 2-methylisoborneol by immunoreaction., J.EICA, 2(2):35-40(1997)..
137. Miyamoto, T., Kuramitsu, Y., Tanaka, Y., Kawata, S., Ohto, T. and Hatano, S., Preparation and properties of monoclonal antibody specific to 2-methylisoborneol., Journal of Japan Society on Water Environment, 20(2):112-116(1997)..
138. Tian, H., Miyamoto, T., Okabe, T., Kuramitsu, Y., Honjoh, K. and Hatano, S., Rapid detection of Salmonella spp. in foods by combination of a new selective enrichment and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase., J. Food Prot., 59, 11, 1158-1163, 59(11):1158-1163, 1996.12.
139. Miyamoto, T., Kawabata, K., Honjoh, K. and Hatano, S., Effects of trehalose on freeze tolerance of baker's yeast., J. Fac. Agr., Kyushu Univ., 41, 1,2, 105-112, 41(1,2):105-112, 1996.12.
140. Hatano, S., Udou, M., Koga, N., Honjoh, K. and Miyamoto, T., Impairment of the glycolytic system and actin in baker's yeast during frozen storage. , Biosci. Biotech. Biochem., 60, 1, 61-64, 60(1):61-64, 1996.12.
141. Honjoh, K., Yoshimoto, M., Joh, T., Kajiwara, T., Miyamoto, T. and Hatano, S. , Isolation and characterization of hardening-induced proteins in Chlorella vulgaris C-27: identification of late embryogenesis abundant proteins. , Plant Cell Physiol., 36, 8, 1421-1430, 36(8):1421-1430, 1995.12.
142. Matsuno, K., Miyamoto, T., Yamaguchi, K., Md. A. Sayed, Kajiwara, T., Hatano, S., Identification of DNA-binding proteins changed after induction of sporulation of Bacillus cereus., Biosci. Biotech. Biochem., 59, 2, 231-235, 59(2):231-235, 1995.12.
143. Joh, T., Honjoh, K., Yoshimoto, M., Funabashi, J., Miyamoto, T., Hatano, S., Molecular cloning and expression of hardening-induced genes in Chlorella vulgaris C-27: the most abundant clone encodes a late embryogenesis abundant protein., Plant Cell Physiol., 36, 1, 85-93, 36(1):85-93, 1995.12.
144. Miyamoto, T., Tian, H., Matsuno, K., Takata, R. and Hatano, S., Application of monoclonal antibodies to dulcitol 1-phosphate dehydrogenase for rapid detection of Salmonella. , J. Food Protection, 58, 8, 847-852, 58(8):847-852, 1995.08.
145. Miyamoto, T., Miyako, K., Nakamura, J. and Hatano, S., Production and partial purification of HEp-2 cell-vacuolation factor derived from Bacillus cereus., Jpn. J. Food Microbiol., 11(2):113-117(1994)..
146. Joh, T., Yoshida, T., Yoshimoto, M., Miyamoto, T., Hatano, S., Composition and positional distribution of fatty acids in polar lipids from Chlorella ellipsoidea differing in chilling susceptibility and frost hardiness. , Physiologia Plantarum, 10.1034/j.1399-3054.1993.890206.x, 89, 2, 285-290, 89:285-290, 1993.12.
147. Takata, R., Yoshimoto, M., Miyamoto, T., Sakamoto, J., Soeda, T. and Hatano, S., Inhibitory monoclonal antibody against japanese radish acid phosphatase., Biosci. Biotech. Biochem., 57, 11, 1924-1928, 57(11):1924-1928, 1993.12.
148. Miyamoto, T., Tamai, R., Tian Hue Ze, Yoshimoto, M. and Hatano, S., Evaluation of fluorogenic assay for the rapid detection of Salmonella in foods., J. Fac. Agr., Kyushu Univ., 38, 1,2, 47-54, 38(1,2):47-54, 1993.12.
149. Joh, T., Yoshimoto, M., Honjoh, K., Miyamoto, T. and Hatano, S., Changes in translatable RNA population during hardening of Chlorella ellipsoidea C-27., J. Fac. Agr., Kyushu Univ., 37, 3,4, 257-263, 37(3,4):257-263, 1993.12.
150. Hatano, S., Joh, T., Miyamoto, T. and Yoshimoto, M., Preparation of protoplasts from Chlorella ellipsoidea C-27., Plant Cell Physiol., 33, 5, 651-655, 33(5):651-655, 1992.12.
151. Yoshimoto, M., Kimura, T., Miyamoto, T., Sakamoto, J. and Hatano, S., Purification and properties of acid phosphatase from japanese radish., Biosci. Biotech. Biochem., 10.1271/bbb.56.147, 56, 1, 147-148, 56(1):147-148, 1992.12.
152. Miyamoto, T., Yonemura, K., Yoshimoto, M., Morinaga, Y. and Hatano, S., Rapid detection of Salmonella by fluorogenic assay. , Jpn. J. Food Microbiol., 8, 3, 143-150, 8(3):143-150, 1991.12.
153. Miyamoto, T., Yonemura, K., Yoshimoto, M. and Hatano, S., Purification and some properties of a novel ethanol dehydrogenase with high activity against dulcitol 1-phosphate from Salmonella typhimurium IFO 12529. , Agric. Biol. Chem., 55, 12, 3045-3051, 55(12):3045-3051, 1991.12.
154. Miyamoto, T., Matsumoto, K., Yoshimoto, M. and Hatano, S., Induction of sporulation during chromosome replication by Bacillus cereus and changes in chromosomal proteins of cells that sporulated., Nippon Nogeikagaku Kaishi, 65(12):1769-1776(1991)..
155. Yoshimoto, M., Okamura, H., Joh, T., Miyamoto, T. and Hatano, S., Changes in soluble and membrane proteins of Chlorella ellipsoidea during early time of hardening.
, J. Fac. Agr., Kyushu Univ., 36, 1,2, 69-77, 36(1,2):69-77, 1991.12.
156. Miyamoto, T., Miwa, H. and Hatano, S., Improved fluorogenic assay for rapid detection of Vibrio parahaemolyticus in foods.
, Appl. Environ. Microbiol., 56, 5, 1480-1484, 56(5):1480-1484, 1990.05.
157. Miyamoto, T., Yasuda, A., Shimoda, M., Fukui, K. and Hatano, S., Identification of genus Bacillus isolated from corn soup by gas chromatography., J. Food Hyg. Soc. Jpn., 31(1):22-29(1990.02)..
158. Okawa, Y., Yoshimoto, M., Miyamoto, T. and Hatano, S., Effect of Azo dyes on proliferation in mouse embryo culture cells., J. Food Hyg. Soc. Jpn., 30(6):496-500(1989)..
159. Miyamoto, T., Sheu, Y-I., Miwa, H. and Hatano, S., A fluorogenic assay for the rapid detection of some Vibrio species including Vibrio parahaemolyticus in foods., J. Food Hyg. Soc., 30, 6, 534-541, 30(6):534-541, 1989.12.
160. Miyamoto, T., Tanaka, R., Kishikawa, T., Mori, S. and Hatano, S., Studies on the chilled storage of corn soup., Sci. Bull. Fac. Agr., Kyushu Univ., 44(1,2):9-15(1989)..
161. Miyamoto, T., Kunitake, K. and Hatano, S., Mechanism of action of an antibacterial factor derived from Bacillus subtilis FHC 402 on Salmonella typhimurium.
, Agric. Biol. Chem., 52, 3, 649-654, 52(3):649-654, 1988.12.
162. Miyamoto, T., Ohyama, K., Yoshimoto, M. and Hatano, S., Mechanism of the combined effects of Bacillus subtilis FHC 402-derived antibacterial factor and hexametaphosphate on Escherichia coli.
, Agric. Biol. Chem., 52, 3, 655-660, 52(3):655-660, 1988.12.
163. Miyamoto, T., Yamada, K. and Hatano, S., Effect of an antibacterial factor derived from Bacillus subtilis FHC 402 on the growth of bacteria and mouse myeloma MPC-11 cells.
, J. Food Hyg. Soc., 28, 5, 364-371, 28(5):364-371, 1987.10.
164. Miyamoto, T., Yoshimoto, M., Tsutsumi, M., Yamada, K. and Hatano, S., Purification and some properties of an antibacterial factor derived from Bacillus subtilis FHC 402.
, Agric. Biol. Chem., 50, 5, 1169-1176, 50(5):1169-1176, 1986.12.
165. Tsutsumi, M., Miyamoto, T., Suda, I. and Watanabe, T., Properties of active substance, BCF, which synergistically enhances the antimicrobial activity of hexametaphosphate. , J. Fac. Agr., Kyushu Univ., 26, 2,3, 71-78, 26(2,3):71-78, 1982.12.