| Katsumi DOI | Last modified date：2024.02.07 |
Professor /
Microbial Genetics /
Department of Bioscience and Biotechnology /
Faculty of Agriculture
Presentations
| 1. | Shiki K., Fujino Y., Doi K., Development of thermostable chloramphenicol acetyltransferase with thermophilic host, Thermus thermophilus, JSBBA West 6th Student Forum, 2023.11. |
| 2. | Xiong S., Nakamura T., Horie Y., Shirazawa T., Chiba K., Katakura Y., Doi K., Apoptotic death of lung cancer cells using holin, lytic protein from bacteriophage, JSBBA West 6th Student Forum, 2023.11. |
| 3. | Muhammad Azka Aufa Ramadhani, Minami Matsunaka, Yasuhiro Fujino, Takeo Iwamoto, Yasuaki Hiromasa and Katsumi Doi, Host Recognition Mechanism of Jumbo Phage vB_BceM_WH1 Infecting Bacillus cereus Group, 第10回ファージ研究会, 2023.08. |
| 4. | Muhammad Azka Aufa Ramadhani, Minami Matsunaka, Yasuhiro Fujino, Takeo Iwamoto, Yasuaki Hiromasa, Katsumi Doi, Host Recognition Mechanism of Jumbo Phage φWH1 Infecting Bacillus cereus Group, 第60回化学関連支部合同九州大会, 2023.07. |
| 5. | Doi K., A New Era in Phage Research, 16th JSPS Association of the Korea-Japan Researcher Network Symposium, 2023.03. |
| 6. | Nakamura T., Horie Y., Shirazawa T., Chiba K., Katakura Y., Doi K, Localized bactelial Holin in cancer cell induces apoptotic death: comparative research of Holin species, JSBBA West 5th Student Forum, 2022.11. |
| 7. | Nagayoshi S., Tanaka R., Mizushima Y., Deguch T., Fujino Y., Doi K., Stability of thermophilic lysogenic phage ΦOH2 isolated from Obama hot spring, JSBBA West 5th Student Forum, 2022.11. |
| 8. | YEUNG Pui Yan, MAEDA Junpei, GODA Asuka, FUJINO Yasuhiro, DOI Katsumi, Investigation of the Function of D-branched Chain Amino Acid (D-BCAA) in Lactobacillus otakiensis, 第58回化学関連支部合同九州大会, 2021.07. |
| 9. | Thanh Nguyen Cong, Fujino Yasuhiro,Tashiro Kosuke, Iwamoto Takeo, Hiromasa Yasuaki, Doi Katsumi, Characterization and genome structure of broad host range phage vB_EkoM5VN infecting Enterobacter and Citrobacter species, 日本農芸化学会２０21年度大会, 2021.03. |
| 10. | IIDA T, FUJINO Y, SUEMATSU Y, DOI K, Development of a new gene expression vector for Thermos thermophilus using a silica-inducible promoter, JSBBA West 3rd Student Forum, 2020.12. |
| 11. | NguyenCongThanh， Yasuhiro Fujino， YasuakiHiromasa， KatsumiDoi, Characterization and genome structure of virulent phage φEM4 infected to Enterobacter sp. M4，a cause of soft rot disease of plants, 日本農芸化学会 2019 年度 西日本・中四国支部合同沖縄大会, 2019.11. |
| 12. | KOJIMA K, GODA A,MAEDA J, FUJINO Y, DOI K, Elucidation of the metabolic mechanism of D-BCAA produced by Lactobacillus otakiensis, JSBBA West 2nd Student Forum, 2019.11. |
| 13. | YOSHIDA N , YAMASAKO A , FUJINO Y , DOI K, Functional analysis of Receptor Binding Protein of Lactococcus phage Q1 isolated from lactic acid fermentation plant, JSBBA West 2nd Student Forum, 2019.11. |
| 14. | Deguchi T, Kim M, Hindra M, Fujino Y, Doi K, Characterization of φOH2, a lysogenic phage infecting Geobacillus kaustophilus, JSBBA West 1st Student Forum, 2018.11. |
| 15. | Shirazawa T, Chiba T, Kuroki M, Katakura Y, Doi K, Localization of the lytic protein Holins to mitochondria induces anti-cancer effect, JSBBA West 1st Student Forum, 2018.11. |
| 16. | Sirinthorn Sunthornthummas,Onanong Pringsulaka,Yasuhiro Fujino, Katsumi Doi, Characterization of Lactobacillus paracasei phageΦT25 from fermented milk in Thailand, 日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会, 2017.09. |
| 17. | Mina KIM, Hindra MARTONO, Yuko NAGAYOSHI, Junpei MAEDA, Yasuhiro FUJINO, Katsumi DOI, Characterization of thermostable lytic proteins from Thermus bacteriophage φOH2, AFELiSA 2016, 2016.11. |
| 18. | Keita Chiba, Michiru Kuroki, Gakuro Harada, Yoshinori Katakura, Katsumi DOI, Apoptotic death of cancer cells using holin, lytic protein from bacteriophage, AFELiSA 2016, 2016.11. |
| 19. | KAYUMBA JIMMY, JUNPEI MAEDA, YASUHIRO FUJINO, KATSUMI DOI, IDENTIFICATION AND CHARACTERIZATION OF LAB ISOLATED FROM RWANDAN CHEESE AND JAPANESE FERMENTED TEA LEAVES (GOISHI-CHA), 第53回化学関連支部合同九州大会, 2016.07. |
| 20. | Nguyen Cong Thanh, 永吉 佑子, 藤野 泰寛, 土居 克実, Isolation and characterization of bacteriophage of Enterobacter asburiae, a cause of soft rot disease of plants in Vietnam, 第52回化学関連支部合同九州大会, 2015.06. |
| 21. | Martono Hindra, Yuko Nagayoshi, Toshihisa Ohshima, Yasuhiro Fujino, Katsumi Doi, Characterization of Novel Thermostable Endolysin from Phage φOH2 , 2014 Molecular Genetics of Bacteria and Phages Meeting, 2014.08, This research attempts to clone a novel endolysin from phage φOH2 and to perform characterization and functional analysis of the enzyme. φOH2 endolysin is found to be an extremely thermostable enzyme carrying PGRP domain, capable of degrading cell wall of bacteria other than phage’s host and withstand treatment in various pH buffers. Further work on this particular enzyme could help in understanding heat resistant mechanism of thermostable endolysin and developing a new antibacterial agent that could target antibiotic-resistant bacteria. . |
| 22. | Yuko NAGAYOSHI, Kenta KUMAGAE, Yasuhiro FUJINO, Toshihisa OHSHIMA, Katsumi DOI, Isolation and characrization of Thermus thermophilus phage φOH3, AFELiSA 2013, 2013.11. |
| 23. | Hirokazu SUZUKI, Junpei KOBAYASHI, Keisuke WADA, Megumi FURUKAWA, Katsumi DOI, Thermostabilization-directed gene evolution using adaptive mutations in the thermophile Geobacillus kaustophilus HTA426, BioMicroWorld 2013, 2013.10. |
| 24. | Hironaga AKITA, Hirokazu SUZUKI, Katsumi DOI, Toshihisa OHSHIMA, Efficient synthesis and stable isotope-labeling of D-branched-chain amino acids using thermostable D-amino acid dehydrogenase, Thermophiles2013, 2013.09. |
| 25. | , [URL]. |
| 26. | Thermus thermophilus TMY, an extreme thermophile, was isolated from a siliceous deposit formed from geothermal water at a geothermal power plant. At concentrations higher than the solubility of amorphous silica (400 to 700 ppm SiO2, at 750), a silica-induced protein (Sip) was expressed in the cell envelope of log-phase cells grown in the presence of supersaturated silicic acid. Molecular weightand pI of Sip were about 35 kDa and 9.5, respectively. Induction of Sip expression occurred within 1 h after the addition of a supersaturating concentration of silicic acid to broth. Production of Sip and its upregulation were in excellent temporal agreement with the silica precipitation, which was observed at silicic acid concentrations greater than 400 ppm in the medium at 75°C. The amino acid sequence of Sip was similar to that of the predicted solute-binding protein of the Fe3+ -ABC transporter in T. thermophilus HB8 and HB27 [2]. Within the genome, sip is situated as a component of the Fbp-type ABC transporter operon, which contains a palindromic structure immediately downstream of sip. This structure is conserved in other T. thermophilus genomes and may function as a terminator that causes definitive Sip expression in response to silica stress. The sip gene was cloned in pET32 and expressed in E. coli. Transformant grown in LB containing 600 ppm silica precipitated silica during their growth (Fig. 1). Purified and heat-treated Sip protein also precipitated silica effectively. Therefore Sip might exhibit their silica precipitation ability. , [URL]. |
| 27. | The genus Thermus are defined as aerobic, heterotrophic, non-motile, pigmented, non-spore-forming, and Gram- negative rods that can grow at temperatures over 70 °C. Previously, we isolated Thermus thermuohilus TMY from the geothermal power plant in Japan and reported that strain TMY induces the precipitation of supersaturated silicic acid during exponeatial growth phase. Notably, supersaturated silicic acid markedly stimulated expression of one cell envelope protein, which is named as silica-induced protein (Sip). The amino acid sequence of Sip showed significant similarity with the solute-binding protein of Fe3+ ABC transporters observed in other Thermus strains, however, little is known about the regulation of gene expression in response to supersaturated silicic acid. To determine the regulation of Sip expression, the transdriptional regulation mechanisms were investigated. Althogh sip operon comprises sip (solute-binding protein), permiase and ATPase, only sip gene was strongly transcribed in the silica-stressed condition. This might result from the fact that the palindromic structure located immidiately downstream of sip could function as the terminator of sip. Primer extension analysis revealed that transcription initiation site of sip is located at 34 bases upstream of start codon. -35 and -10 element of the sip promoter showed meaningful similarity to those of &A promoters commonly seen in T. thermophilus. Sip trnscription was enhanced by the addition of supersaturated silicic acid, however, sip was also transcribed in the iron-condition. Due to the negative charge of colloidal silica in silica-stressed condition, iron ions might be trapped by colloidal silica. Thermus cells might receive the signals of the existence of supersaturated silicic acid as the iron deficiency caused by colloidal silica. This mechanism of silica-responsible promoter could serve us more convenient and effective genetic tools for thermophile and must shed new light on bacterial biosilicification studies. , [URL]. |
| 28. | , [URL]. |
| 29. | , [URL]. |
| 30. | , [URL]. |
| 31. | Study on Temporal and Spatial Expression of the Sporulation-inhibitory Gene of Conjugative Plasmid During Differentiation in Streptomyces. Award lecture for Hamada Award of Japan Society for Actinomycetes in 2004.. |
| 32. | Analysis of ABC transporter gene in Streptomyces azureus. |
| 33. | Site-specific integration of lysogenic phage SAt2 in Streptomyces azureus. |
| 34. | Temporal and Spatial Expression of DNA translocase Gene in Streptomyces azureus. |
| 35. | Characterization of plasmid pLKS in Lactobacillus plantarum NGRI0101. |
| 36. | Identification of plasmid affected its host growth in Lactobacillus plantarum NGRI0101. |
| 37. | Genetic analysis of ABC transporter gene in Streptomyces azureus. |
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