Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Ken-ichi Honjoh Last modified date:2020.10.26

Associate Professor / Division of Food Science and Biotechnology / Department of Bioscience and Biotechnology / Faculty of Agriculture

1. Yu Zhang, Kumiko Shigemura, Hoang Minh Duc, Cunkuan Shen, Hung Hsin Huang, Jun Sato, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Effects of bacteriophage on inhibition and removal of mixed biofilm of enterohemorrhagic Escherichia coli O157:H7 and O91:H-, LWT, 10.1016/j.lwt.2020.109945, 134, 2020.12, This study describes the characterization of bacteriophage FP43, active against pathogenic and non-pathogenic strains of Escherichia coli, and its ability to inhibit and remove a mixed biofilm of enterohemorrhagic E. coli O157:H7 and O91:H-. Phage FP43 is a member of the Myoviridae family, having a dsDNA genome consisting of 169,248 bp with good stability. It has a short latent period and large burst size. Phage FP43 decreased the formation of biofilm, comprising E. coli O157:H7 and O91:H-, by 82.4% at 30 °C. After incubation for 6 h with FP43, viable counts of E. coli O157:H7 and total cells in the biofilm were reduced by 2.76 and 2.85 log, respectively. In planktonic cells, viable E. coli O157:H7 and total counts decreased by 3.44 and 3.62 log, respectively, after incubation with the phage for 4 h. Moreover, more than 60% of an established, mixed biofilm was removed after a 6 h exposure to the phage, in which E. coli O157:H7 and total viable counts decreased by 2.07 and 1.93 log, respectively. These results suggest that bacteriophage FP43 has broad host range against both pathogenic and non-pathogenic E. coli, and potential to reduce viable counts of both enterohemorrhagic E. coli O157:H7 and E. coli O91 in mixed-strain biofilm..
2. Apisada Kitichalermkiat, Mao Katsuki, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken ichi Honjoh, Takahisa Miyamoto, Effect of epigallocatechin gallate on gene expression of Staphylococcus aureus, Journal of Global Antimicrobial Resistance, 10.1016/j.jgar.2020.06.006, 22, 854-859, 2020.09, Objectives: Staphylococcus aureus is an important nosocomial pathogen that produces various extracellular toxins. Epigallocatechin gallate (EGCg) is a polyphenol that is abundant in green tea. EGCg displays strong antibacterial activity against Gram-positive bacteria. The effect of EGCg on gene expression by S. aureus was investigated to clarify the mechanism underlying its antibacterial action. Methods: Microarray analysis was performed on S. aureus treated with or without 500 mg/L EGCg. Differentially expressed genes were identified and their changes at the transcription level were confirmed using real-time quantitative polymerase chain reaction (qPCR). The membrane potential of cells treated with or without EGCg were observed under fluorescence microscopy. Results: Microarray analysis revealed that EGCg treatment of S. aureus resulted in increased and decreased transcription of 75 and 72 genes, respectively. Increased transcription exceeding 1-log2-fold change of genes related to membrane transport included gntP, gntK, rumA, SAOUHSC_02723, SAOUHSC_01311, and vraS. Decreased transcription was observed in genes involved in toxin production and stress response (hlgA, SAOUHSC_01110, hly, hlgB, efb, and hlgC). All changes in transcription were confirmed using real-time qPCR. The membrane potential of S. aureus treated with 500 mg/L EGCg markedly decreased, indicating that EGCg damaged the cell membrane. Conclusions: S. aureus increases the transcription of genes involved in membrane transport to recover membrane function. EGCg can potentially serve as a natural antibacterial agent to control the growth and toxin production of S. aureus..
3. Yoshimitsu Masuda, Erika Sakamoto, Ken Ichi Honjoh, Takahisa Miyamoto, Role of toxin-antitoxin-regulated persister population and indole in bacterial heat tolerance, Applied and environmental microbiology, 10.1128/AEM.00935-20, 86, 16, 1-10, 2020.08, YafQ is an endoribonuclease toxin that degrades target gene transcripts such as that of tnaA, a gene encoding tryptophanase to synthesize indole from tryptophan. DinJ is the cognate antitoxin of YafQ, and the YafQ-DinJ system was reported to regulate persister formation by controlling indole production in Escherichia coli. In this study, we investigated the role of YafQ-DinJ, indole production, and persister population in bacterial heat tolerance. yafQ (ΔyafQ), dinJ (ΔdinJ), and tnaA (ΔtnaA) single-gene knockout mutants showed approximately 10-fold higher heat tolerance than wild-type (WT) E. coli BW25113. Persister fractions of all mutants were slightly larger than that of the WT. Interestingly, these persister cells showed an approximately 100-fold higher heat tolerance than normal cells, but there was no difference among the persister cells of all mutants and the WT in terms of heat tolerance. Indole and its derivatives promoted a drastic reduction of bacterial heat tolerance by just 10 min of pretreatment, which is not sufficient to affect persister formation before heat treatment. Surprisingly, indole and its derivatives also reduced the heat tolerance of persister cells. Among the tested derivatives, 5-iodoindole exhibited the strongest effect on both normal and persister cells..
4. Hoang Minh Duc, Hoang Minh Son, Pham Hong Ngan, Jun Sato, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Isolation and application of bacteriophages alone or in combination with nisin against planktonic and biofilm cells of Staphylococcus aureus, Applied Microbiology and Biotechnology, 10.1007/s00253-020-10581-4, 104, 11, 5145-5158, 2020.06, Staphylococcus aureus is a notorious foodborne pathogen since it has ability to produce variety of toxins including heat-stable enterotoxin, form biofilm, and acquire resistance to antibiotics. Biocontrol of foodborne pathogens by lytic bacteriophages garners increasing interest from both researchers and food industry. In the present study, 29 phages against S. aureus were successfully isolated from chicken, pork, and fish. Characterization of the isolates revealed that phage SA46-CTH2 belonging to Podoviridae family had a number of features suitable for food industry applications such as wide host range, short latent period, large burst size, high stress tolerance, and a genome free of virulence genes. Furthermore, phage SA46-CTH2 alone or in combination with nisin exhibited great efficacy in reducing planktonic and biofilm cells of S. aureus at various conditions tested. The combination of phage SA46-CTH2 and nisin was also found to be able to inhibit the regrowth of S. aureus at both 37 and 24 °C.Key points• A total of 29 S. aureus phages were successfully isolated from fish, pork, and chicken products. • Phage SA46-CTH2 was characterized by host range, morphology, and genome sequencing. • SA46-CTH2 significantly reduced both planktonic and biofilm cells of S. aureus. • Combination of SA46-CTH2 and nisin inhibited the regrowth of S. aureus..
5. Hoang Minh Duc, Hoang Minh Son, Hazel Pang Shu Yi, Jun Sato, Pham Hong Ngan, Yoshimitsu Masuda, Ken ichi Honjoh, Takahisa Miyamoto, Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157:H7 in different food matrices, Food Research International, 10.1016/j.foodres.2020.108977, 131, 2020.05, Salmonella Enteritidis, Salmonella Typhimurium, and Escherichia coli O157:H7 are the most important foodborne pathogens, causing serious food poisoning outbreaks worldwide. Bacteriophages are increasingly considered as novel antibacterial agents to control foodborne pathogens. In this study, 8 Salmonella phages and 10 E. coli O157:H7 phages were isolated from chicken products. A polyvalent phage PS5 capable of infecting S. Enteritidis, S. Typhimurium, and E. coli O157:H7 was further characterized and its efficacy in reducing these foodborne pathogens was evaluated in in vitro and in foods. Morphology, one-step growth, and stability assay showed that phage PS5 was a myovirus, with relatively short latent periods, large burst sizes, and high stability. Genome sequencing analysis revealed that the genome of PS5 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, and virulence factors. In broth, phage PS5 significantly decreased the viable counts of all the three bacterial hosts by more than 1.3 log CFU/mL compared to controls after 2 h of incubation at 4 °C and 24 °C. In foods, treatment with PS5 also resulted in significant reductions of viable counts of all the three bacterial hosts compared to controls at temperatures tested. This is the first report on single phage capable of simultaneously controlling S. Enteritidis, S. Typhimurium and E. coli O157:H7 in both in vitro and in foods..
6. Mahmoud Ge Zayda, Yoshimitsu Masuda, Ahmed M. Hammad, Ken ichi Honjoh, Abdelrahman M. Elbagory, Takahisa Miyamoto, Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese, International Dairy Journal, 10.1016/j.idairyj.2020.104646, 104, 2020.05, We investigated the characteristics of Staphylococcus aureus isolates causing bovine subclinical mastitis (SCM), and their genetic relatedness with the isolates obtained from Egyptian cheese. Twenty-five S. aureus isolates were identified from 150 SCM milk and 75 cheese samples. The antibiogram revealed multidrug-resistant (MDR) isolates. Fifteen isolates were categorised as methicillin-resistant. Antimicrobial resistance and virulence genes were detected. Spa typing and SCCmec classification were performed. More than 50% of isolates were found carrying human-specific virulence determinants, while other isolates characterised were presumed to be of bovine-origin. Spa-types t304, t688, t084 corresponded isolates from SCM milk and cheese samples; the similar genotypes from SCM and cheese displayed divergence in virulence traits. Moreover, our results revealed the novel spa-type t18546. Animals and dairy food could be a reservoir for transformative changes in S. aureus virulence, leading to the emergence of virulent MDR strains that may become potential public-health threats..
7. Cunkuan Shen, Md Tariqul Islam, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Transcriptional changes involved in inhibition of biofilm formation by ε-polylysine in Salmonella Typhimurium, Applied Microbiology and Biotechnology, 10.1007/s00253-020-10575-2, 2020.04, The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism underlying ε-polylysine-mediated inhibition of biofilm formation, the transcriptional profiles of ε-polylysine-treated and untreated Salmonella Typhimurium cells were comparatively analysed. The genome-wide DNA microarray analysis was performed using Salmonella Typhimurium incubated with 0.001% ε-polylysine in 0.1% Bacto Soytone at 30 °C for 2 h. The expression levels of genes involved in curli amyloid fibres and cellulose production, quorum sensing, and flagellar motility were downregulated, whereas those of genes associated with colanic acid synthesis were upregulated after treatment with ε-polylysine. The microarray results were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore, treatment with ε-polylysine decreased the production of colanic acid in Salmonella Typhimurium. The findings of this study improved our understanding of the mechanisms underlying ε-polylysine-mediated biofilm inhibition and may contribute to the development of new disinfectants to control biofilm during food manufacturing and storage..
8. Yue Guo, Xiaowen Cui, Liushu Ou, Chika Isowaki, Yoshimitsu Masuda, Ken-ichi Honjoh and Takahisa Miyamoto, Effects of Sucrose on Heat Resistance and Gene Expression in Salmonella Typhimurium, Food Science and Technology Research, 10.3136/fstr.25.903, 25, 6, 903-913, 2019.11.
9. Aye Thida Maung, Tahir Noor Mohammadi, Satoko Nakashima, Pei Liu, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Antimicrobial resistance profiles of Listeria monocytogenes isolated from chicken meat in Fukuoka, Japan, International Journal of Food Microbiology, 10.1016/j.ijfoodmicro.2019.05.016, 304, 49-57, 2019.09, In this study, the antimicrobial resistance profiles of L. monocytogenes isolated from chicken meat in Fukuoka in 2017 were compared with the isolates of 2012. A total of 85 and 50 chicken meat samples, including different body parts, were collected from different supermarkets in Fukuoka in 2012 and 2017, respectively. Detection, isolation, identification, and characterization of L. monocytogenes were performed according to the conventional methods. Forty-five among 85 samples (53%) were positive for L. monocytogenes in 2012, while 12 among 50 samples in 2017 (24%) tested positive. One hundred fifty-three and 29 L. monocytogenes strains were isolated in 2012 and 2017, respectively. The serotypes of isolates in 2012 were 1/2a (21.5%), 1/2b (73.9%), 1/2c (1.5%), and 4b/4e (3.1%). In contrast, the 2017 isolates showed 1/2a (48.3%) and 1/2b (51.7%) serotypes. While all isolates in 2012 were positive for hlyA (listeriolysin O) in the PCR assay with hlyA primer set 7, only 17 hlyA positive isolates were seen in 2017. Moreover, 75 isolates with different ribotypes in 2012 and 29 isolates in 2017, respectively, were tested for antimicrobial susceptibility by broth microdilution for 18 different antimicrobial agents. Most of the 2012 and 2017 isolates displayed antimicrobial susceptibility. However, among the 2012 and 2017 isolates, 98.7% and 100% of the isolates were resistant to cefoxitin, 57.3% and 95.7% to fosfomycin, 72.0% and 82.6% to oxacillin, 8.0% and 17.4% to clindamycin, respectively. In addition, 2.7% of the isolates in 2012 were resistant to flomoxef and 4.3% of the isolates in 2017 to linezolid. Multidrug resistance (MDR) to 3 or more antimicrobials was observed in 35/75 (46.7%) isolates of 2012 and 19/23 (82.6%) in 2017. Detection of antimicrobial resistance (AMR) genes by PCR showed that the resistant isolates of 2012 were positive for mecA (96.3%) and ermC (83.3%), whereas the resistant isolates in 2017 screened positive for mecA (94.7%) and mefA (25.0%). Other cfxA, ermA, ermB, fosA, fosB, and fosC genes were absent in the PCR assay for any of the isolates. This study investigated for the first time the change in the L. monocytogenes contamination of chicken meat and antibiotic resistance of the isolated L. monocytogenes strains in Fukuoka, Japan, in the course of 5 years. Although the contamination rate of L. monocytogenes in 2017 was found to be lower than that in 2012, AMR of the isolates in 2017 was higher..
10. Pham Thi Vinh, Yui Shinohara, Akifumi Yamada, Hoang Minh Duc, Motokazu Nakayama, Tadahiro Ozawa, Jun Sato, Yoshimitsu Masuda, Ken Ichi Honjoh, Takahisa Miyamoto, Baicalein inhibits Stx1 and 2 of EHEC
Effects of baicalein on the cytotoxicity, production, and secretion of shiga toxins of enterohaemorrhagic escherichia coli, Toxins, 10.3390/toxins11090505, 11, 9, 505-505, 2019.08, Shiga toxin-producing enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen. Baicalein (5,6,7-trihydroxylflavone), a flavone isolated fromthe roots of Scutellaria baicalensis, is considered as a potential antibacterial agent to control foodborne pathogens. Among seven compounds selected by in silico screening of the natural compound database, baicalein inhibited the cytotoxicity of both Shiga toxins 1 and 2 (Stx1 and Stx2) against Vero cells after pretreatment at 0.13 mmol/L. In addition, baicalein reduced the susceptibility of Vero cells to both Stx1 and Stx2. Real-time qPCR showed that baicalein increased transcription of stx1 but not of stx2. However, baicalein had no effects on production or secretion of Stx1 or Stx2. Docking models suggested that baicalein formed a stable structure with StxB pentamer with low intramolecular energy. The results demonstrate that inhibitory activity of baicalein against the cytotoxicity of both Stx1 and Stx2 might be due to of the formation of a binding structure inside the pocket of the Stx1B and Stx2B pentamers..
11. Xiaowen Cui, Chuanqi Hu, Liushu Ou, Yumiko Kuramitsu, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Transcriptional analysis on heat resistance and recovery from thermal damage in Salmonella under high salt condition, LWT, 10.1016/j.lwt.2019.02.056, 106, 194-200, 2019.06, Sodium chloride maintains osmotic pressure of living cells including bacteria. Heat treatment is common for decontamination of bacteria in food. In this study, effects of NaCl on heat resistance of Salmonella Typhimurium were investigated. After cultivation in TSB containing 0.5% (TSB), 4% (4SC) and 8% (8SC) NaCl, S. Typhimurium cells were heated at 60 °C for 20 min. Total viable counts including intact cells and injured but recoverable cells determined by the plating method using TSA of S. Typhimurium cultured in 4SC and 8SC were significantly higher than those of the cells cultured in TSB. Meanwhile, changes of gene transcription were analyzed by DNA microarray. Transcription of genes involved in the colanic acid synthesis largely increased after cultivation in 4SC and 8SC. The amount of colanic acid significantly increased in the cells cultured in 4SC compared to that in M9-glucose medium. After recovery culture for 3 h, the genes involved in the phage shock response strongly up-regulated, suggesting contribution of these gene products in recovery of heat injured cells. The outcome of this study contributes to understand the mechanism of cross protection in Salmonella..
12. T. Noor Mohammadi, A. T. Maung, J. Sato, T. Sonoda, Yoshimitsu Masuda, Ken-Ichi Honjoh, Takahisa Miyamoto, Mechanism for antibacterial action of epigallocatechin gallate and theaflavin-3,3′-digallate on Clostridium perfringens, Journal of Applied Microbiology, 10.1111/jam.14134, 126, 2, 633-640, 2019.02, Aim: The purpose of this study was to clarify the mechanism of the antibacterial action of two high potential and natural food additives, epigallocatechin gallate (EGCg) and theaflavin-3,3′-digallate (TF3), on Clostridium perfringens. Methods and Results: Minimal inhibitory concentrations were determined by the serial dilution method. Afterwards, the cells were treated with 250 or 1000 mg l−1 of EGCg and 125 or 500 mg l−1 of TF3 and morphological changes were observed and cell sizes were also measured under fluorescence microscopy. Our results showed that TF3 had a twice stronger antibacterial activity than EGCg against C. perfringens. Phase-contrast and fluorescence microscopy confirmed that the bacterial cells elongated without DNA segregation and septum formation in the presence of 250 mg l−1 EGCg. While in the higher concentration of EGCg and TF3, cell growth was suppressed. Bacterial cells reached to around 12 μm after the 24 h incubation with 250 mg l−1 EGCg, but the cells were shorter than the control at 1000 mg l−1 of EGCg. After washing and incubating the elongated cells in fresh medium, DNA segregated at 2 h of incubation. The average cell length decreased gradually and reached the normal size at 8 h. Conclusion: It seems that EGCg at a low concentration affected the proteins involved in the septum formation, DNA segregation and cell division. Furthermore, the high concentration of EGCg and TF3 seemed to cause stronger cellular damage to C. perfringens. Significance and Impact of the Study: These polyphenols are widely distributed in all higher plants especially in tea plants, and people tend to use natural food additives rather than synthetic ones. EGCg and TF3, as natural food additives, can prevent C. perfringens food poisoning along with other potential health benefits..
13. Apisada Kitichalermkiat, Masahiro Kurahachi, Ai Nonaka, Motokazu Nakayama, Kanami Shimatani, Naofumi Shigemune, Takashi Tsugukuni, Jun Hitomi, Jun Sato, Takumi Sonoda, Yoshimitsu Masuda, Ken ichi Honjoh, Takahisa Miyamoto, Effects of Epigallocatechin Gallate on Viability and Cellular Proteins of Staphylococcus aureus, Food Science and Technology Research, 10.3136/fstr.25.277, 25, 2, 277-285, 2019.01, This study investigated the effect of epigallocatechin gallate (EGCg) on Staphylococcus aureus to determine its mechanism of antibacterial action. Adsorption of EGCg on the cell envelope of S. aureus after EGCg treatment was demonstrated using a FITC-labeled antibody specific to EGCg. After EGCg treatment of S. aureus for 4 h, abnormalities in septum formation and cell segregation were observed at concentrations greater than 250 mg/L, and debris presumed to arise from cell destruction or leakage of cytoplasmic materials was observed around the cells at 500 mg/L. Two-dimensional electrophoresis of proteins prepared from EGCg-treated S. aureus cells revealed the presence of 18 protein spots that disappeared or showed markedly decreased intensity compared to those from control cells. These proteins included DnaK, elongation factor G, DNA-directed RNA polymerase, l-lactate dehydrogenase, pyruvate dehydrogenase, and acetate kinase. Furthermore, S. aureus showed decreased glucose uptake after EGCg treatment. These results suggest that EGCg inhibits the functions of cell-envelope proteins, and it causes cellular damage and disruption of the cells in S. aureus..
14. Ayumi Musou-Yahada, Ken-Ichi Honjoh, Kenta Yamamoto, Takahisa Miyamoto, Hideaki Ohta, Utilization of single nucleotide polymorphism-based allele-specific PCR to Identify shiikuwasha (citrus depressa hayata) and calamondin (Citrus madurensis Lour.) in processed juice, Food Science and Technology Research, 10.3136/fstr.25.19, 25, 1, 19-27, 2019.01, To develop a method for the identification of shiikuwasha (Citrus depressa Hayata) and calamondin (Citrus madurensis Lour.), trnL-trnF and trnT-trnL intergenic spacer regions of their chloroplast DNA were amplified using PCR and the nucleotide sequences were determined. In each region, a single nucleotide polymorphism (SNP) site specific to the respective citrus species (shiikuwasha and calamondin) was found. For species discrimination using PCR, two forward primers containing the allele-specific SNP site at the 3’-end and a mismatched nucleotide at the 3
base from the 3’-end were designed. The allele-specific forward primers specific to shiikuwasha and calamondin were respectively designated CiDeLF-F and CiMaTL-F. To confirm the specificity of the designed primers, PCR was carried out with DNA prepared from citrus peel or hand-squeezed juice as the template. Results showed that shiikuwasha and calamondin fruits and juices were identifiable by PCR using the allele-specific primers. Furthermore, this allele-specific PCR method can be applied to industrially processed and concentrated juice by amplifying DNA in advance..
15. Ken-ichi HONJOH, Yin LIN, Kiyomi JO, Yuri IWAIZAKO, Masayuki MAEDA, Nobuyuki KIJIMA, and Takahisa MIYAMOTO, Possible contamination routes of Listeria monocytogenes to leaf lettuce during cultivation, Food Science and Technology Research, 24, 5, 911-920, 2018.09.
16. Ken-ichi HONJOH, Hitomi OKANO, Aya KAWABATA, Masaru KUROKAWA, Taiki KIMURA, Takeshi MACHIDA, Yoshimitsu MASUDA and Takahisa MIYAMOTO, Freezing tolerance of Lactuca sativa and induction of CBF and GolS genes during cold treatment, Journal of Faculty of Agriculture, Kyushu University, 63, 2, 249-257, 2018.09.
17. Xiao-guang Zhang, Sachiko Tsuji, Hayato Kitaoka, Mitsuru Tamai, Hiroshi Kobayashi, Ken-ichi HONJOH, and Takahisa MIYAMOTO, Preparation and characterization of monoclonal antibodies suitable for detection of foodborne pathogens by biosensor, Journal of Faculty of Agriculture, Kyushu University, 63, 2, 319-330, 2018.09.
18. Hoang Minh Duc, Hoang Minh Son, Ken-Ichi Honjoh, Takahisa Miyamoto, Isolation and application of bacteriophages to reduce Salmonella contamination in raw chicken meat, LWT - Food Science and Technology, 10.1016/j.lwt.2018.01.072, 91, 353-360, 2018.05, Chicken meats are considered as main sources associated with Salmonella infections in humans. In this study, lytic phages against Salmonella were isolated and examined for their efficacy to control Salmonella. Eighteen lytic phages were isolated from raw chicken skin and gizzard. Five phages belonging to Myoviridae and Siphoviridae families were characterized and selected for bacterial challenge tests. The treatment of raw chicken breast samples contaminated with S. Enteritidis and S. Typhimurium at 8 °C by the cocktail of five phages significantly reduced (P < 0.05) viable counts by 1.41 and 1.86 log CFU/piece, respectively. When incubated at 25 °C, the highest reductions of viable counts of S. Enteritidis and S. Typhimurium in the phage-treated samples were 3.06 and 2.21 log CFU/piece, respectively (P < 0.05). These data suggested that the phages isolated from raw chicken meats are potential agents for controlling Salmonella in raw meats..
19. Currently, injury of foodborne pathogen in agricultural environment is unclear. So, we observed survival of foodborne pathogens on common procedure for growing vegetable by cultivation on two types of agar medium that possess different selectivity. In case of Salmonella enterica serovar Infantis (SI) on the process of manure preparation, injury was estimated by cultivation on selective agar medium and resuscitation on non-selective agar medium. While in Listeria monocytogenes (LM) on cultivation environment of tomato, physiological changes were confirmed by salt content in agar medium. In manure environment, insufficient elevation of temperature in piled feedstock caused injured SI. LM survived for long term and part of these survived as injured LM in soil and on the surface of tomato fruits. It might be possible to estimate injured SI in actual condition of manure preparation by above procedure. On the contrary, there are practical difficulties for discriminating injured LM in actual agricultural environment by only salt sensitivity..
20. XIAOWEN CUI, HSU-MING SHERMAN WEN, YOSHIMASA KINOSHITA, SHOTA KOISHI, CHIKA ISOWAKI, LIUSHU OU, YOSHIMITSU MASUDA, KEN-ICHI HONJOH, TAKAHISA MIYAMOTO, Role of Phage Shock Protein in Recovery of Heat-injured Salmonella, Biocontrol Science,, 23, 1, 17-25, 2018.03.
21. Munenori Furuta, Takayuki Nasu, Kouichi Umeki, Duc Hoang Minh, Ken-ichi Honjoh, and Takahisa Miyamoto, Characterization and application of lytic bacteriophages against Campylobacter jejuni isolated from poultry in Japan, Biocontrol Science,, 22, 4, 213-221, 2017.12, [URL].
22. Xiaoguang Zhang, Sachiko Tsuji, Hayato Kitaoka, Hiroshi Kobayashi, Mitsuru Tamai, Ken-Ichi Honjoh, Takahisa Miyamoto, Simultaneous Detection of Escherichia coli O157
H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor, Journal of Food Science, 10.1111/1750-3841.13843, 82, 10, 2357-2363, 2017.10, Abstract: Detection of foodborne pathogens at very low levels is still a challenge. A custom-built multichannel surface plasmon resonance (SPR) biosensor and simultaneous enrichment broth (SEB) were used to develop a simultaneous detection method for 3 important foodborne pathogens, Escherichia coli O157:H7 (O157:H7), Salmonella enteritidis, and Listeria monocytogenes, at a very low level. These 3 foodborne pathogens at a very low level (14, 6, and 28 CFU/25 g (mL) for O157:H7, S. enteritidis, and L. monocytogenes, respectively) were inoculated in SEB and incubated at 37 ˚C for 24 h. Sample prepared from the simultaneous enrichment culture was analyzed using the multichannel SPR biosensor and sensor chip immobilized with polyclonal antibodies specific to each of the target pathogens. O157:H7, S. enteritidis, and L. monocytogenes in chicken were detected simultaneously at an inoculum dose of 14, 6, and 28 CFU/25 g, respectively. Our method using a custom-built multichannel SPR biosensor and enrichment in SEB is expected as a rapid and simultaneous detection method for low levels of O157:H7, S. enteritidis, and L. monocytogenes in food. Practical Application: Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications..
23. Takahisa Miyamoto, Xiaoguang Zhang, Yuuki Ueyama, Kitichalermkiat Apisada, Motokazu Nakayama, Yasuto Suzuki, Tadahiro Ozawa, Asako Mitani, Naofumi Shigemune, Kanami Shimatani, Koji Yui, Ken-Ichi Honjoh, Development of novel monoclonal antibodies directed against catechins for investigation of antibacterial mechanism of catechins, Journal of Microbiological Methods, 10.1016/j.mimet.2017.03.014, 137, 6-13, 2017.06, Catechins are major polyphenolic compounds of green tea. To investigate mechanism for antibacterial action of catechins, 11 monoclonal antibodies (MAbs) were raised against a 3-succinyl-epicatechin (EC)-keyhole limpet hemocyanin (KLH) conjugate. Amino acid sequences of variable regions determined for MAbs b-1058, b-1565, and b-2106 confirmed their innovative character. MAb b-1058 strongly interacted with its target substances in the following order of magnitude: theaflavin-3,3′-di-O-gallate (TFDG) > theaflavin-3-O-gallate (TF3G) ≥ theaflavin-3′-O-gallate (TF3’G) > gallocatechin gallate (GCg) > penta-O-galloyl-β-D-glucose (PGG) > epigallocatechin gallate (EGCg), as determined using surface plasmon resonance (SPR) on MAb-immobilized sensor chips. The affinity profiles of MAbs b-1058 and b-2106 to the various polyphenols tested suggested that flavan skeletons with both carbonyl oxygen and hydroxyl groups are important for this interaction to take place. S. aureus cells treated with EGCg showed green fluorescence around the cells after incubation with FITC-labeled MAb b-1058. The fluorescence intensity increased with increasing concentrations of EGCg. These MAbs are effective to investigate antibacterial mechanism of catechins and theaflavins..
24. Duc Hoang Minh, Son Hoang Minh, Ken-Ichi Honjoh, Takahisa Miyamoto, Isolation and bio-control of Extended Spectrum Beta-Lactamase (ESBL)-producing Escherichia coli contamination in raw chicken meat by using lytic bacteriophages, LWT - Food Science and Technology, 10.1016/j.lwt.2016.04.013, 71, 339-346, 2016.09, Extended Spectrum Beta-Lactamase-producing Escherichia coli (ESBLEC) were isolated from 12/13 (92.3%) raw chicken meat samples by using selective culture and PCR. Of the 27 ESBLEC analyzed, 33.3% (9/27) of isolates were positive for ESBL of CTX-M group 1 followed by TEM (22.2%), SHV (22.2%), CTX-M group 2 (11.1%), and CTX-M group 9 (11.1%). None of ESBLEC tested were positive for stx1, stx2, eae, ehxA, saa, and subAB genes of Shiga toxin-producing E. coli. Among 22 isolated phages, phages PBL66-CL1 and PBL116-CS6 infected 21 (77.7%) and 20 (74%) of 27 ESBLEC isolates examined, respectively. The remaining phages lysed less than 50% of the hosts tested. Compared to non-treatment, the treatment of isolate EBL116 in the broth medium with phage cocktail of PBL66-CL1 and PBL116-CS6 at 25 °C and 5 °C after 6 h significantly reduced bacterial viable counts by 5.06 and 1.33 log CFU/mL, respectively. When the treatment was performed on raw chicken meat samples, viable counts of EBL116 were also decreased by 2.02 and 1.67 log CFU/4 cm2 meat piece after 6 h at 25 °C and 5 °C, respectively. This study demonstrates a high prevalence of ESBLEC in raw chicken meat and possible use of lytic phages as bactericide for controlling ESBLEC..
25. Md Tariqul Islam, Aya Ogura, Chikako Machida, Noriko Morinaga, Ken-Ichi Honjoh, Takahisa Miyamoto, Effects of ϵ-polylysine and milk serum protein on the attachment and decontamination of Salmonella Enteritidis on lettuce and radish sprouts, Food Science and Technology Research, 10.3136/fstr.22.703, 22, 5, 703-711, 2016.06, To understand the effects of pretreatment of lettuce during cultivation with 0.001% '-polylysine (PL) in combination with 0.25% milk serum protein (MSP) on the attachment of Salmonella Enteritidis, lettuce leaves were contaminated with S. Enteritidis after a 1-day treatment with food additives, and harvested after cultivation for 1 day. Viable S. Enteritidis counts on lettuce leaves pretreated with the PL and MSP mixture were significantly reduced from 5.7 log CFU/g to 1 log CFU/g after decontamination by washing with water and a subsequent treatment with NaClO. The viable S. Enteritidis counts on radish sprouts grown for 7 d in the presence of 0.01% PL from seeds inoculated with S. Enteritidis were reduced to 3.1 log CFU/50 sprouts after NaClO decontamination. These counts were significantly lower (P < 0.05) than those of plants without additive(s). The treatment with additive(s) did not affect the ascorbic acid and chlorophyll contents of both plants..
26. Honjoh, K., Iwaizako, Y., Lin, Y., Kojima, N., Miyamoto, T., Possibilities of contamination of tomato fruits by Listeria monocytogenes during cultivation, Food Science and Technology Research,, 22, 3, 349-357, 2016.05, [URL].
27. Hoang Minh Son, Hoang Minh Duc, Ken-Ichi Honjoh, Takahisa Miyamoto, Identification of the newly identified subtilase cytotoxin-encoding gene (Subab2-2) among clinical shigatoxin-producing Escherichia coli isolates, Canadian Journal of Microbiology, 10.1139/cjm-2015-0519, 61, 12, 990-994, 2015.09, Subtilase cytotoxin (SubAB) is an important virulence factor of eae-negative Shiga toxin-producing Escherichia coli (STEC). Three variants of SubAB-encoding genes have been reported in the literature; however, the newly described subAB variant (subAB2-2) was found only in STEC strains from deer meat, sheep, and some wild animals. In this study, subAB variants were detected by PCR and DNA sequencing in 5 out of 12 (41.6%) eae-negative STEC strains isolated from patients. Most subAB-positive STEC strains (80%) harbored the subAB1 gene. The subAB2-2 gene was detected for the first time in the clinical STEC O128:H2 strain. Other virulence genes including stx1a, stx1c, stx2b, ehxA, and tia were also detected in this strain. The DNA sequence analyses of the subAB1 and subAB2-2 genes of the clinical STEC strains showed 99% and 100% identity to those of the reference strains 98NK2 and LM27558stx2, respectively. This is the first report on the detection of the subAB2-2 gene in a clinical STEC isolate..
28. Son Hoang Minh, Etsuko Kimura, Duc Hoang Minh, Ken-Ichi Honjoh, Takahisa Miyamoto, Virulence characteristics of shiga toxin-producing Escherichia coli from raw meats and clinical samples, Microbiology and Immunology, 10.1111/1348-0421.12235, 59, 3, 114-122, 2015.03, Shiga toxin producing Escherichia coli (STEC) are dangerous foodborne pathogens. Foods are considered as important sources for STEC infection in human. In this study, STEC contamination of raw meats was investigated and the virulence factors of 120 clinical STEC strains characterized. STEC was detected in 4.4% of tested samples. Among 25 STEC strains from meats, five strains (20%) were positive for the eae gene, which encodes intimin, an important binding protein of pathogenic STEC. The remaining strains (80%) were eae-negative. However, 28% of them possessed the saa gene, which encodes STEC agglutinating adhesin. The ehxA gene encoding for enterohemolysin was found in 75% of the meat strains and the subAB gene, the product is of which subtilase cytotoxin, was found in 32% of these strains. The stx2a gene, a subtype of Shiga toxin gene (stx), was the most prevalent subtype among the identified meat STEC bacteria. None of the meat STEC was O157:H7 serotype. Nevertheless, 92% of them produced Shiga toxin (Stx). Among 120 clinical STEC strains, 30% and 70% strains harbored single and multiple stx subtypes, respectively. Most clinical STEC bacteria possessed eae (90.8%) and ehxA (96.7%) genes and 92.5% of them showed Stx productivity. Our study shows that some raw meat samples contain non-O157 STEC bacteria and some strains have virulence factors similar to those of clinical strains..
29. Approaches of Improvement of Freezing-Stress Tolerance of Yeast.
30. Honjoh, K., Mishima, T., Kido, N., Shimamoto, M., Miyamoto, T., Investigation of possible contamination routes of Salmonella via soils and the effects of mulch for avoiding its contamination to leafy lettuce plants during cultivation, Food Science and Technology Research,, 20, 5, 961-969, 2014.09, [URL].
31. Md Tariqul Islam, Akinobu Oishi, Chikako Machida, Aya Ogura, Shoken Kin, Honjoh, K., Miyamoto, T., Combined effects of selected food additives on adhesion of various foodborne pathogens onto microtiter plate and cabbage leaves, Food Control, 10.1016/j.foodcont.2014.05.034, 46, 233-241, 2014.06.
32. Xiaoguang Zhang, Hayato Kitaoka, Sachiko Tsuji, Mitsuru Tamai, Hiroshi Kobayashi, Ken-ichi Honjoh, Takahisa Miyamoto, Development of a simultaneous detection method for food borne pathogens using surface plasmon resonance biosensors, Food Science and Technology Research, 10.3136/fstr20.317, 20, 2, 317-325, 2014.02.
33. Miyamoto, T., Toyofuku, T., Tachiki, N., Kimura, E., Zhou, T., Ozawa, T., Nakayama, M., Shigemune, N., Shimatani, K., Tokuda, H., Honjoh, K., Specific inhibition of cytotoxicity of Shiga-like toxin 1 of enterohemorrhagic Escherichia coli by gallocatechin gallate and epigallocatehin gallate, Food Control, 42, 263-269, 2014.02.
34. Zhang, X., Tsuji, S., Kitaoka, H., Tamai, M., Kobayashi, H., Honjoh, K., Miyamoto, T., Effects of pretreatments on detection of E. coli O157:H7 by SPR biosensor, Journal of Faculty of Agriculture, Kyushu University, 58, 2, 307, 58, 2, 307-312, 2013.11.
35. Miyamoto, T., Mekada, Y., Kurahachi, M., Umeno, M., Nakayama, M., Shigemune, N., Tsugukuni, T, Tokuda, H., Tachibana, H., Honjoh, K., A highly sensitive method for quantifying gallocatechin gallate and its epimer using a catechin-specific peptide, Food Control, 29, 1, 162-166, 29, 1, 162-166, 2013.01.
36. Mishima, T., Kido, N., Nakashima, S., Miyaji, N., Soli, K.W., Ken-ichi Honjoh, Md. Bari, L., Takahisa Miyamoto, Investigation of possible situation of internalization of salmonella enteritidis in tomato fruits and bacterial survival during tomato plant cultivation , Food Sci. Technol. Res., 18, 6, 869-877, 2012.11.
37. Machida, T., Ishibashi, A., Kirino, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Chloroplast NADPH-dependent thioredoxin reductase from Chlorella vulgaris alleviates environmental stresses in yeast together with 2-Cys peroxiredoxin, PLoS ONE, 7, 9, e45988, 2012.09.
38. Wen, H.-M., Naito, K., Kinoshita, Y., Kobayashi, H., Honjoh, K., Tashiro, K., Miyamoto, T., Changes in transcription during recovery from heat injury in Salmonella typhimurium and effects of BCAA on recovery, Amino Acids, 42, 6, 2059-2066, 42, 6, 2059-2066, 2012.06.
39. Liu, P., Mizue, H., Fujihara, K., Kobayashi, H., Kamikado, H., Tanaka, T., Honjoh, K., Miyamoto, T., A new rapid real-time PCR method for detection of Listeria monocytogenes targeting the hlyA gene, Food Sci. Technol. Res., 18, 1, 47-57, 2012.01.
40. Soli, K.W., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K., Miyamoto, T., Application of a combined decontamination method for fresh produce using SAHW, sucrose fatty acid ester and microbubbles, Food Sci. Technol. Res., 17, 6, 555-559, 2011.11.
41. Miyamoto, T., Kawagishi, J., Oishi, A., Shimotsu, S., Mishima, T., Kobayashi, H., Honjoh, K., Inhibition of adhesion of several bacteria onto microtiter plate by selected food additives, Jpn. J. Food Microbiol., 28, 3, 157-166, 2011.10.
42. Effects of commercial kitchen detergents on adhesion and viability of bacteria.
43. Machida, T., Honjoh, K., Aso, A., Yamamoto, M., Iio, M., Miyamoto, T., Trehalose 6-phosphate synthase and trehalose 6-phosphate phosphatase from Nicotiana tabacum function in trehalose biosynthesis and environmental stress tolerance of yeast, Journal of Faculty of Agriculture, Kyushu University, 55, 2, 261-268, 55, 2, 261-268, 2010.11.
44. Soli, K.W., Motomatsu, A., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K., Miyamoto, T., Comparison of the bactericidal effect of slightly acidic hypochlorous water with that of conventional sterilizers, Journal of Faculty of Agriculture, Kyushu University, 55, 2, 275, 55, 2, 275-280, 2010.11.
45. Soli, K.W., Yoshizumi, A., Yamakawa, M., Mishima, T., Honjoh, K., Miyamoto, T., Bacterial contamination and microflora of several fresh produce, Journal of Faculty of Agriculture, Kyushu University, 55, 2, 269, 55, 2, 269-273, 2010.11.
46. Li, R., Harada, T., Honjoh, K., Miyamoto, T., Phylogenetic analysis and Shiga toxin production profiling of Shiga toxinproducing/enterohemorrhagic Escherichia coli clinical isolates, Microbial Pathogenesis, 49, 246-251, 49: 246-251, 2010.09.
47. Honjoh, K., Matsuura, K., Machida, T., Nishi, K., Nakao, M., Yano, T., Miyamoto, T., Iio, M., Enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: Co-expression of cDNA clones, from Cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in Saccharomyces cerevisiae, Amino Acids, 10.1007/s00726-009-0328-6., 38, 4, 1173-1183, 38(4): 1173-1183, 2010.04.
48. Soli, K.W., Yoshizumi, A., Motomatsu, A., Yamakawa, M., Yamasaki, M., Mishima, T., Miyaji, N., Honjoh, K., Miyamoto, T., Decontamination of fresh produce by the use of slightly acidic hypochorous water following pretreatment with sucrose fatty acid ester under microbubble generation, Food Control, 21, 9, 1240-1244, 21(9) 1240-1244, 2010.04.
49. Machida, T., Ohashi, N., Mimura, A., Honjoh, K., Iio, M., Miyamoto, T., Chloroplastic glucose 6-phosphate dehydrogenase from Chlorella vulgaris alleviates freezing and menadione-indued oxidative stresses in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 55, 1, 29-38, 2010.03.
50. Miyamoto, T., Nakayama, M., Shigemune, N., Tokuda, H., Matsushita, T., Furuta, K., Mekada, Y., Honjoh, K. Application of green tea extract as food preservative to improve shelf life of overnight pickled cucumber, Nippon Shokuhin Kagaku Kogaku Kaishi, 56, 12, 660-664 (2009).
51. Machida, T., Honjoh, K., Shimizu, H., Yamamoto, M., Iio, M., Miyamoto, T., Expression of a gene encoding a functional glycosyl hydrolase, trehalase, from Nicotiana tabacum in Saccharomyces cerevisiae, Journal of the Faculty of Agriculture, Kyushu University , 54, 2, 297-304, 2009.11.
52. Honjoh, K., Hashimoto, Y., Shimotsu, S., Wen, H.-M., Kiriki, M., Naito, K., Tokugawa, M., Satake, E., Kobayashi, H., Miyamoto, T., Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ∆agfA and ∆bcsA mutants of Salmonella Enteritidis; ∆ahpC, ∆ahpF, and ∆katG mutants of S. Typhimurium; and ∆rpoE, ∆rpoH, and ∆rpoS mutants of S. Enteritidis and S. Typhimurium, Journal of the Faculty of Agriculture, Kyushu University , 54, 2, 421-432, 2009.11.
53. Kobayashi, H., Kubota, J., Fujihara, K., Honjoh, K., Iio, M., Fujiki, N., Nakabe, M., Oda, S., Takasu, K., Nakanishi, H., Miyamoto, T., Simultaneous enrichment of Salmonella spp, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes by single broth and screening of the pathogens by multiplex real-time PCR , Food Sci. Technol. Res., Vol. 15, No.4, 427-438, 2009.07.
54. Machida, T., Kato, E., Ishibashi, A., Sato, J., Kawasaki, S., Niimura, Y., Honjoh, K., Miyamoto, T., Expression pattern of a chloroplast NADPH-dependent thioredoxin reductase in Chlorella vulgaris during hardening and its interaction with 2-Cys peroxiredoxin., Biosci. Biotechnol. Biochem., 73(3), 695-701, 2009.03.
55. Combined effects of surfactants and preservatives on the antibacterial activity of green tea extracts.
56. Watanabe, Y., Yamada, N., Machida, T., Honjoh, K., Kuwano, E., Influence of cold hardening on chlorophyll and carotenoid in Chlorella vulgaris, J. Fac. Agr., Kyushu Univ., 54(1): 195-200, 2009.02.
57. Honjoh, K., Fujihara, K., Haraguchi, T., Ono, Y., Kobayashi, H., Hiwaki, H., Kamikado, H., Jang, S.S., Ryu, S., Miyamoto, T., Subtyping of Listeria monocytogenes based on nucleotide polymorphism in clpC, inlA, hlyA, and plcA genes and rapid identification of L. monocytogenes genetically similar to clinical isolates, Food Sci. Technol. Res., Vol. 14, No.6, 557-564, 2008.12.
58. Machida, T., Murase, H., Kato, E., Honjoh, K., Matsumoto, K., Miyamoto, T., Iio, M., Isolation of cDNAs for hardening-induced genes from Chlorella vulgaris by suppression subtractive hybridization., Plant Science, 175, 3, 238-246, 2008.09.
59. Machida, T., Kato, E., Ishibashi, A., Ohashi, N., Honjoh, K., Miyamoto, T., Molecular characterization of low-temperaure-inducible NTR-C in Chlorella vulgaris, Nucleic Acids Symposium Series, No. 51, 463-464, 2007.11.
60. The effect of Lunularic acid on the freezing tolerance of Chlorella vulgaris IAM C-27.
61. Honjoh, K., Machida, T., Nishi, K., Matsuura, K., Soli, K.W., Sakai, T., Ishikawa, H., Matsumoto, K., Miyamoto, T., Iio, M., Improvement of freezing and oxidative stress tolerance in Saccharomyces cerevisiae by taurine, Food Sci. Technol. Res., 13,2, 145-154, 2007.06.
62. Honjoh,K., Machida, T., Hagisako, T., Suga, K., Yonekura, M., Shimizu, H., Ohashi, N., Miyamoto, T., Hatano, S., Iio, M., Molecular cloning and characterization of a cDNA for low-temperature inducible cytosolic glucose 6-phosphate dehydrogenase gene from Chlorella vulgaris and expression of the gene in Saccharomyces cerevisiae, Plant Science, 172, 649-658, 2007.03.
63. Miyamoto, T., Shimizu, Y., Kobayashi, H., Honjoh, K., Iio, M.,, Studies of collagen binding with immobilized Salmonella enteritidis and inhibiton with synthetic and naturally occurring food additives by a surface plasmon resonance biosensor., Sensors and Materials., 15, 8, 453-466, 15 (8): 453-466, 2005.01.
64. Miyamoto, T., Nakayama, M., Sasaki, C., Sadakari, K., Itoh, Y., Fujimoto, A., Honjoh, K., Iio, M., Simple identification of some Bacillus species and related genera in food by RAPD analysis combined with morphological observation., Biocontrol Science, 10(1/2) 45-53, 2005.01.
65. Kobayashi, H., Miyamoto, T., Hashimoto, Y., Kiriki, M., Motomatsu, A., Honjoh, K., Iio, M., Identification of factors involved in recovery of heat-injured Salmonella Enteritidis., J. Food Prot., 68, 5, 932-941, 68 (5): 932-941, 2005.01.
66. Miyamoto T., Kamikado, H., Sasaki, C., Sadakari, K., Honjoh, K., and Iio, M.,, An attempt to identify Bacillus cereus by PCR., Biocontrol Science, 9(3): 69-75, 2004.01.
67. Honjoh, K., Mimura, A., Kuroiwa, E., Hagisako, T., Suga, K., Shimizu, H., Dubey, R.S., Miyamoto, T., Hatano, S., and Iio, M., Purification and characterization of two isoforms of glucose 6-phosphate dehydrogenase (G6PDH) from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.67.1888, 67, 9, 1888-1896, 67(9): 1888-1896, 2003.09.
68. Development of a rapid and simple method for measuring total viable bacterial counts by flow cytometry, Bokin Bobai, Vol. 31, No.7, 357-363 (2003).
69. Miyamoto, T., Kamikado, H., Kobayashi, H., Honjoh, K., and Iio, M., Immunomagnetic flow cytometric detection of staphylococcal enterotoxin B in raw and dry milk, J. Food Protection., 66, 7, 1222-1226, 66(7):1222-1226, 2003.07.
70. Honjoh, K., Suga, K., Shinohara, F., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M., Preparation of protoplasts from Chlorella vulgaris K-73122 and cell wall regeneration of protoplasts from C. vulgaris K-73122 and C-27, J. Fac. Agr., Kyushu Univ., 47, 2, 257-266, 47(2): 257-266, 2003.02.
71. Shimizu, H., Furuya, N., Suga, K., Honjoh, K., Miyamoto, T., Hatano, S., and Iio, M., Freezing tolerance of transgenic tobacco with increased content of unsaturated fatty acid by expressing the CvFad2 or CvFad3 gene, J. Fac. Agr., Kyushu Univ., 47, 2, 307-317, 47(2): 307-317, 2003.02.
72. Suga, K., Honjoh, K., Furuya, N., Shimizu, H., Nishi, K., Shinohara, F., Hirabaru, Y., Maruyama, I., Miyamoto, T., Hatano, S., and Iio, M.,, Two low-temperature-inducible Chlorella genes for D12 and w-3 fatty acid desaturase (FAD): Isolation of D12 and w-3 fad cDNA clones, expression of D12 fad in Saccharomyces cerevisiae , and expression of w-3 fad in Nicotiana tabacum, Biosci. Biotechnol. Biochem., 66, 6, 1314-1327, 66(6): 1314-1327, 2002.06.
73. Miyamoto, T., Ichioka, N., Sasaki, C., Kobayashi, H., Honjoh, K., Iio, M. and Hatano, S., Polymerase chain reaction assay for detection of Escherichia coli O157:H7 and Escherichia coli O157:H-, J. Food Protection, 65, 1, 5-11, 65(1),5-11 (2002), 2002.01.
74. Miyamoto, T., Sayed, Md. A., Sasahara, R., Sukimoto, K., Umezaki, A.,Honjoh, K., Iio, M. and Hatano, S., Cloning and overexpression of Bacillus cereus penicillin-binding protein 3 gene in Escherichia coli, Biosci. Biotechnol. Biochem., 66, 1, 44-50, 66(1): 44-50, 2002.01.
75. Honjoh,K., Shimizu, H., Nagaishi, N., Matsumoto, H., Suga, K., Miyamoto, T., Iio, M., and Hatano, S., Improvement of freezing tolerance in transgenic tobacco leaves by expressing the hiC6 gene, Biosci. Biotechnol. Biochem., 10.1271/bbb.65.1796, 65, 8, 1796-1804, 65(8): 1796-1804, 2001.08.
76. Honjoh, K., Matsumoto, H., Shimizu, H., Ooyama, K., Tanaka, K., Oda, Y., Takata, R., Joh, T., Suga, K., Miyamoto, T., Iio, M. and Hatano, S., Cryoprotective activities of group3 late embryogenesis abundant proteins from Chlorella vulgaris C-27, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1656, 64, 8, 1656-1663, 64(8): 1656-1663, 2000.08.
77. Kim, S.-I., Miyamoto, T., Honjoh, K., Iio, M., and Hatano, S., Molecular cloning, overproduction and characterization of the Bacillus cereus IMP dehydrogenase, Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1210, 64, 6, 1210-1216, Vol.64, No.6, 1210-1216, 2000.06.
78. Trevanich, S., Miyamoto, T., Harada, Y., Honjoh, K., and Hatano, S., Rapid detection of enterotoxigenic Escherichia coli O6 in water by using monoclonal antibody and a photon-counting TV camera, J. Food Protection, 63, 4, 534-538, Vol.63(4), 534-538, 2000.04.
79. Miyamoto, T., Sukimoto, K., Sayed, Md.A., Kim, S.-I., Honjoh, K., and Hatano, S., Detection of penicillin-binding proteins in Bacillus cereus by using biotinylated ß-lactams,, J. Fac. Agr., Kyushu Univ., 44, 3-4, 299-307, 44(3,4): 299-307 (2000), 2000.03.
80. Studies on freezing injury of unwashed and washed cells of Escerichia coli O157:H7, Jpn. J. Food Microbiology, 17(2), 127-133,2000.
81. Miyamoto, T., Trevanich, S., Honjoh, K., Hatano, S., Rapid detection of Salmonella spp. by PCR amplification of Salmonella specific region in gatD gene, 日本食品微生物学会雑誌, 第16巻2号,99-109., 1999.02.
82. K. Honjoh, Y. Oda, R. Takata, T. Miyamoto, and S. Hatano, Induction of the hiC6 gene, which encodes a homologue of a late embryogenesis abundant (LEA) protein, enhances freezing tolerance of yeast, Journal of Plant Physiology, Vol.155, No.4,5, 509-512, 1999.01.
83. Rapid detection method of Salmonella-specific PCR product by DNA-immobilized quartz crystal microbalance. Jpn. J. Food Microbiol., 16(1) 57-63. (1999).
84. T. Miyamoto, Y. Kuramitsu, A. Ookuma, S. Trevanich, K. Honjoh, S. Hatano., Rapid detection of counting of viable bacteria in vegerables and environmental water using a photon-counting TV camera, J. Food Protection, Vol.61, No.10, 1312-1316, 1998.10.
85. T. Miyamoto, H.Z. Tian, T. Okabe, S . Trevanich, K. Asoh, S. Tomoda, K. Honjoh, S. Hatano., Application of random amplified polymorphic DNA analysis for detection of Salmonella spp. in foods., J. Food Prot., 61, 7, 785-791, Vol.61, No.7, 785-791, 1998.07.
86. R. Takata, Y. Miyamoto, K. Honjoh, T. Soeda, J. Sakamoto, T. Miyamoto, S. Hatano., Antibody fragments as inhibitors of Japanese radish acid phosphatase, Biosci. Biotechnol. Biochem., 10.1271/bbb.62.1041, 62, 6, 1041-1047, Vol.62, No.6, 1041-1047, 1998.06.
87. T. Miyamoto, K. Matsuno, M. Imamura, S.I. Kim, K. Honjoh, S. Hatano., Purification and some properties of IMP dehydrogenase of Bacillus cereus., Microbiol. Res., 153, 1, 23-27, Vol. 153, No.1, 23-27, 1998.01.
88. Hatano, S., Honjoh, K., Miyamoto, T., Rapid detection of Salmonella spp. in foods by combination of RAPD analysis and QCM technique., 4th International ANQUE chemistry conference., 1998.01.
89. Miyamoto, T., Yamaguchi, K., Sayed,M.D., Sasahara, R., Honjoh, K., and Hatano, S., Penicillin-binding protein sensitive to cephalexin in sporulation of Bacillus cereus., Microbiol. Research. , 152, 3, 227-232, 152(3), 227-232., 1997.03.
90. Miyamoto, T., Imamura, M., Matsuno, K., Kim, S.-I., Honjoh, K., and Hatano, S., Involvement of IMP dehydrogenase activity in induction of sporulation of Bacillus cereus., Microbiol. Research. , 152, 3, 277-280, 152(3), 277-280., 1997.03.
91. Tian, H., Miyamoto, T., Okabe, T., Kuramitsu, Y., Honjoh, K., and Hatano, S., Rapid detection of Salmonella spp. in Foods by combination of a new selective enrichment and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase, J. Food Protect., 59, 11, 1158-1163, 59(11), 1158-1163, 1996.11.
92. Miyamoto, T., Kawabata, K., Honjoh, K., and Hatano, S., Effects of trehalose on freeze tolerance of baker's yeast., J. Fac. Agr., Kyushu Univ. , 41, 1-2, 105-112, 41(1・2), 105-112., 1996.09.
93. Hatano, S., Udou, M., Koga, N., Honjoh, K., Miyamoto, T., Impairment of the glycolytic system and actin in baker's yeast during frozen storage., Biosci. Biotech. Biochem., 60, 1, 61-64, 60(1), 61-64, 1996.01.
94. Honjoh, K., Yoshimoto, M., Joh, T., Kajiwara, T., Miyamoto, T., Hatano, S., Isolation and characterization of hardening-induced proteins in Chlorella vulgaris C-27: Identification of late embryogenesis abundant proteins, Plant Cell Phyisiol., 36, 8, 1421-1430, 36(8), 1421-1430, 1995.12.
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