| 片倉 喜範（かたくら よしのり） | データ更新日：2023.09.27 |
教授 ／
農学研究院
生命機能科学部門
システム生物工学講座
| 1. | Azusa Yamada, Kota Watanabe, Yuri Nishi, Mugihito Oshiro, Yoshinori Katakura, Kenji Sakai, Yukihiro Takahiro, Scalp bacterial species influences SIRT1 and TERT expression in keratinocytes., Biosci. Biotech. Biochem., 2023.09. |
| 2. | Sonam Tamrakar, Dongmei Wang, Eri Hiraki, Chunguang Han, Yang Ruan, Ahmed E Allam, Yhiya Amen, Yoshinori Katakura, Kuniyoshi Shimizu, Deacylated derivative of hericenone C treated by lipase shows enhanced neuroprotective properties compared to its parent compound, Molecules, 2023.06. |
| 3. | Asuka Ishibashi, Miyako Udono, Mikako Sato, Yoshinori Katakura, Molecular mechanisms for the carnosine-induced activation of muscle–brain interaction, Nutrients, doi: 10.3390/nu15061479, 15, 1479, 2023.06. |
| 4. | Tsukasa Fujiki, Ryosuke Shinozaki, Miyako Udono, Yoshinori Katakura, Identification and functional evaluation of polyphenols that induce regulatory T cells, Nutrients, doi.org/10.3390/nu14142862, 14, 2862, 2022.07.  |
| 5. | Akene Mihara, Tomoyuki Ohara, Jun Hata, Sanmei Chen, Takanori Honda, Sonam Tamrakar, Akiko Isa, Dongmei Wang, Kuniyoshi Shimizu, Yoshinori Katakura, Koji Yonemoto, Tomohiro Nakao, Takanari Kitazono, Toshiharu Ninomiya, Association of serum s-adenosylmethionine, s-adenosylhomocysteine, and their ratio with the risk of dementia and death in a community, Sci. Rep., 10.1038/s41598-022-16242-y, 12, 1, 12427, 2022.07. |
| 6. | Tsukasa Fujiki, Shin-Ei Matsumoto, Kenji Kishihara, Yoshinori Katakura, Age-related functional decline of human B cells, Cytotechnology, 10.1007/s10616-021-00513-z, 24, 319-327, 2022.01, [URL]. |
| 7. | Merieme Ledrhem, Miku Nakamura, Miyu Obitsu, Kinue Hirae, Jun Kameyama, Hafida Bouamama, Chemeseddoha Gadhi, Yoshinori Katakura, Essential oils derived from Cistus species activate mitochondria by inducing SIRT1 expression in human keratinocytes, leading to senescence inhibition, Molecules, 10.3390/molecules27072053, 27, 2053, 2022.03, [URL], シスタス属由来エッセンシャルオイルが皮膚細胞を活性化することを明らかにした。. |
| 8. | Yuki Morita, Yoshinori Katakura, Kaoru Takegawa, Yujiro Higuchi, Correlative localization analysis between mRNA and EGFP-fused protein by single-molecule FISH using an egfp probe in Aspergillus oryzae, Front. Fungal Biol., 2021.09. |
| 9. | Hiromi Ieiri, Natsuki Kameda, Junko Naito, Takanori Kawano,Norihisa Nishida, Madoka Takahashi, Yoshinori Katakura, Paramylon extracted from Euglena gracilis EOD-1 augmented the expression of SIRT1, Cytotechnology, 2021.09. |
| 10. | Ryo Inotsuka, Miyako Udono, Atsushi Yamatsu, Mujo Kim, Yoshinori Katakura, Exosome-mediated activation of neuronal cells triggered by γ-aminobutyric acid (GABA), Nutrients, 13, 2544, 2021.08, GABAにより分泌誘導された腸管由来エクソソームが脳細胞を活性化することを明らかにした。. |
| 11. | Manal Zefzoufi, Rabiaa Fdil, Hafida Bouamama, Chemseddoha Gadhi, Yoshinori Katakura, Abdelkarim Mouzdahir, Khadija Sraidi, Effect of extracts and isolated compounds derived from Retama monosperma (L.) Boiss. on anti-aging gene expression in human keratinocytes, J. Ethnopharmacol., 280, 114451, 2021.08. |
| 12. | Mizuki Ogawa, Miyako Udono, Kiichiro Teruya, Norihisa Uehara, Yoshinori Katakura, Exosomes Derived from Fisetin-treated Keratinocytes Mediate Hair Growth Promotion, Nutrients, 2021.06, ポリフェノールフィセチンが、皮膚表皮由来のエクソソーム分泌を誘導し、毛包幹細胞を活性化させ、育毛を誘導することを明らかにした。. |
| 13. | Wei-Ting Tsai, Yuki Nakamura, Taiki Akasaka, Yoshinori Katakura, Yasutake Tanaka, Bungo Shirouchi, Zhe Jiang, Xingyu Yuan, Masao Sato, Soyasaponin ameliorates obesity and reduces hepatic triacylglycerol accumulation by suppressing lipogenesis in high-fat diet-fed mice, J. Food Sci., 86, 5, 2103-2117, 2021.02. |
| 14. | Kayoko Abe, Shuntaro Yamashita, Kohei Kitazaki, Yoshinori Katkarua, Homogenization in water improves the recovery rate of imidazole dipeptide in meat, Food Sci. Tech. Res., 27, 2, 335-339, 2021.02. |
| 15. | 久保智里、小川瑞紀、上原範久、片倉喜範, Fisetin promotes hair growth by augmenting TERT expression, Front. Cell. Dev. Biol., 2020.10, ポリフェノールフィセチンが、育毛促進効果を示した。. |
| 16. | 蕏塚涼、内村佳奈子、山津敦史、＠金武祚、片倉喜範, γ-aminobutylic acid (GABA) activates neuronal cells by inducing the secretin of exosomes from intestinal cells., Food Funct., 2020.10, GABAが腸脳相関を活性化する分子メカニズムを明らかにした。. |
| 17. | Shohei Yoshimoto, Hiromitsu Morita, Miho Matsuda, Yoshinori Katakura, Masato Hirata, Shuichi Hashimoto, NFAT5 promotes oral squamous cell carcinoma progression in a hyperosmotic environment, Lab. Invest., 101, 38-50, 2020.08. |
| 18. | Qiushi Li, Yujiro Higuchi, Kana Tanabe, Yoshinori Katakura, Kaoru Takegawa, Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae, J. Biosci. Bioeng., 129, 5, 573-580, 2020.05. |
| 19. | Boonsong Wungsintaweekul, Kayoko Abe, Rintaro Koga, Yoshinori Katakura, Kohei Torikai, Antimicrobial and anti-oxidative of 12-arylbenzoacridines, Indones. J. Chem., https://doi.org/10.22146/ijc.53411, 20, 5, 1199-1205, 2020.05. |
| 20. | Yuki Morita, Futa Kikumatsu, Yujiro Higuchi, Yoshinori Katakura, Kaoru Takegawa, Characterization and functional analysis of ERAD-related AAA+ ATPase Cdc48 in Aspergillus oryzae, Fungal Biol., 124, 801-813, 2020.05. |
| 21. | Yuka Sugihara, Shiori Onoue, Kosuke Tashiro, Mikako Sato, Takanori Hasegawa, Yoshinori Katakura, Carnosine induces intestinal cells to secrete exosomes that activate neuronal cells, PLoS One, 14, 5, e0217394, 2019.05, カルノシンがエクソソームを介して腸脳相関を活性化する分子基盤を明らかにした。. |
| 22. | Jun Hata, Tomoyuki Ohara, Yoshinori Katakura, Kuniyoshi Shimizu, Shuntaro Yamashita, Daigo Yoshida, Takanori Honda, Yoichiro Hirakawa, Mao Shibata, Satoko Sakata, Takanari Kitazono, Satoru Kuhara, Toshiharu Ninomiya, Association between serum β-alanine and risk of dementia: the Hisayama Study, Am. J. Epidemiol., 1881, 1637-1645, 2019.05, 血中βアラニンが認知症発症のマーカーになりうることを明らかにした。. |
| 23. | Zhao Chong, Haruka Matsuo, Shiori Onoue, Hiroaki Yamamoto, Hideyuki Ito, Yoshinori Katakura, Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation, Journal of Functional Foods, 54, 119-127, 2019.03, 皮膚表皮細胞における長寿遺伝子SIRT1を活性化するポリフェノールが、紫外線により誘導されるDNA損傷を修復した。. |
| 24. | Hui Tan, Chong Zhao, Qinchang Zhu, Yoshinori Katakura, Hiroyuki Tanaka, Koichiro Ohnuki, Kuniyoshi Shimizu, Ursolic Acid Isolated from the Leaves of Loquat (Eriobotrya japonica) Inhibited Osteoclast Differentiation through Targeting Exportin 5, J. Agric. Food Chem., 67, 2, 3333-3340, 2019.03. |
| 25. | Gakuro Harada, Shiori Onoue, Chisato Inoue, Sakae Hanada, Yoshinori Katakura, Delphinidin-3-glucoside suppresses lipid accumulation in HepG2 cells, Cytotechnology, 70, 6, 1707-1712, 2018.12. |
| 26. | Momoko Hamano, Yurina Haraguchi, Tomoko Sayano, Chong Zyao, Yashiho Arimoto, Yui Kawano, Kazuki Moriyasu, Miyako Udono, Yoshinori Katakura, Takuya Ogawa, Hisanori Kato, Shigeki Furuya, Enhanced vulnerability to oxidative stress and induction of inflammatory gene expression in Phgdh-deficient fibroblasts, FEBS Open, 8, 914-922, 2018.06. |
| 27. | Keishi Kadooka, Mikako Sato, Takashi Matsumoto, Satoru Kuhara, Yoshinori Katakura, Tatsuya Fujimura, Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes, Cytotechnology, 70, 3, 983-992, 2018.06. |
| 28. | Zhao Chong, Haruka Matsuo, Mai Kuroda, Shuntaro Yamashita, Gopal Prasad Parajuli, Hira Kaji Manandhar, Kuniyoshi Shimizu, Yoshinori Katakura, Mushroom extract inhibits ultravilet B-induced cellular senescence in human keratinocytes, Cytotechnology, 70, 3, 1001-1008, 2018.06. |
| 29. | ＃原田額郎、＃尾上詩織、＃井上千聡、＃花田栄、片倉喜範, Delphinidin-3-glucoside suppresses lipid accumulation in HepG2 cells, Cytotechnol., 70, 1707-1712, 2018.06. |
| 30. | Yusuke Togo, Yujiro Higuchi, Yoshinori Katakura, Kaoru Takegawa, Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae, Sci. Rep., 7, 1, 15757, 2017.11. |
| 31. | Qiong Ding, Kitora Tanigawa, Jun Kaneko, Mamoru Totsuka, Yoshinori Katakura, Etsuko Imabayashi, Hiroshi Matsuda, Tatsuhiro Hisatsune, Anserine/carnosine supplementation preserves blood flow in the prefrontal brain of elderly people carrying APOE e4, Aging Dis, 9, 1199, 2017.09. |
| 32. | Yoshinori Katakura, Mamoru Totsuka, Etsuko Imabayashi, Hiroshi Matsuda, Tatsuhiro Hisatsune, Anserine/carnosine supplementaion suppresses the expression of the inflammatory chemokine CCL24 in peripheral blood mononuclear cells from elderly people, Nutrients, 9, 334-345, 2017.09. |
| 33. | 井上千聡、Chong Zhao, Yumi Tsuduki, 鵜殿美弥子、Lixang Wang, Masatoshi Nomura, 片倉喜範, SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes, npj Aging Mech. Dis., 3, 11, 2017.08, 独自に同定した細胞老化因子SMARCD1が、肝臓での脂肪滴合成に関わることを明らかにした。. |
| 34. | Kaoru Fujii, Kayoko Abe, Keishi Kadooka, Takashi Matsumoto, 片倉 喜範, Carnosine activates the CREB pathway in Caco-2 cells, Cytotechnology, 2017.06. |
| 35. | Shuntaro Yamashita, Mikako Sato, Takashi Matsumoto, Keishi Kadooka, Takanori Hasegawa, Tatsuya Fujimura, 片倉 喜範, Mechanisms of carnosine-induced activation of neuronal cells, Biosci. Biotechnol. Biochem., 82, 4, 683-688, 2017.04. |
| 36. | Shuntaro Yamashita, Kaoru Fujii, Chong Zhao, Hiroshi Takagi, 片倉 喜範, Involvement of the NFX1-repressor complex in PKC-delta-induced repression of hTERT transcription, JOURNAL OF BIOCHEMISTRY, 10.1093/jb/mvw038, 160, 5, 309-313, 2016.11. |
| 37. | Tomoko Sayano, Yuki Kawano, Wataru Kusada, Yashiho Arimoto, Kayoko Ezaki, Momoko Hamano, Miyako Udono, 片倉 喜範, Takuya Ogawa, 加藤久典, Yoshio Hirabayashi, Shigeki Furuya, Adaptive response to L-serine deficiency is mediated by p38 MAPK activation via deoxysphinganine in normal fibroblasts, FEBS Open Bio, 6, 303-316, 2016.04. |
| 38. | Zhao Chong, Takenori Sakaguchi, Kosuke Fujita, Hideyuki Ito, Norihisa Nishida, Nagatomo Akifumi, Yukimasa Tanaka, 片倉 喜範, Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation, OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 10.1155/2016/2927131, 2016, 2927131, 2016.04, ポリフェノールが、SIRT3の活性化を通じて活性酸素種を抑制することを示した。. |
| 39. | 片倉 喜範, Lactobacillus brevis T2102 suppresses the growth of colorectal cancer cells by activating SIRT1, Journal of Functional Foods, 2016.01, 乳酸菌T2102株が腸管上皮細胞でのSIRT1発現の増強を通じて、がんを抑制することを見出した。. |
| 40. | 片倉 喜範, Effect of Anserine/Carnosine Supplementation on Verbal Episodic Memory in Elderly People, Journal of Alzheimers Disease, 50, 149-159, 2015.12, イミダゾールジペプチド高含有エキスを用いて、中高齢者を対象としたランダム化群間比較二重盲検試験を実施した。その結果、イミダゾールジペプチド摂取群において、言語性記憶の低下抑制効果（記録力の維持）が確認された。. |
| 41. | 片倉 喜範, Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells, SCIENTIFIC REPORTS, 10.1038/srep17342, 5, 2015.11, 細胞老化因子として独自に同定したATP6V0A2が、ゴルジ体の生合成の維持を介して細胞老化に関わっていることを明らかにした。. |
| 42. | 片倉 喜範, In vitro neuroprotective activities of compounds from Angelica shikokiana Makino, Molecules, 20, 4813-4823, 2015.04. |
| 43. | 片倉 喜範, A structure-activity relationship study on antiosteoclastogenesis effect of triterpenoids from the leaves of loquat (Eriobotrya japonica), Phytomedicine, 20, 4, 498-503, 2015.04. |
| 44. | 片倉 喜範, Mechanisms and consequences of carnosine-induced activation of intestinal epithelial cells, JOURNAL OF FUNCTIONAL FOODS, 10.1016/j.jff.2014.12.024, 13, 32-37, 2015.03. |
| 45. | 片倉 喜範, Kibizu concentrated liquid suppresses the accumulation of lipid droplets in 3T3-L1 cells., Cytotechnology, 10.1007/s10616-015-9849-x, 2015.02. |
| 46. | 片倉 喜範, Molecular mechanisms for the p38-induced cellular senescence in normal human fibroblast, JOURNAL OF BIOCHEMISTRY, 10.1093/jb/mvu040, 156, 5, 283-290, 2014.11. |
| 47. | 片倉 喜範, 藤井薫, 小磯綾加, 平川標, 山下万貴子, Identification of anti-allergic lactic acid bacteria that suppress Ca2+ influx and histamine release in human basophilic cells, JOURNAL OF FUNCTIONAL FOODS, 10.1016/j.jff.2014.07.006, 10, 370-376, 2014.09. |
| 48. | 片倉 喜範, FOXO3a Potentiates hTERT Gene Expression by Activating c-MYC and Extends the Replicative Life-Span of Human Fibroblast, PLOS ONE, 10.1371/journal.pone.0101864, 9, 7, e101864, 2014.07, 長寿遺伝子FOXO3aが、cーmyc依存的にテロメラーゼを増強することを分子レベルで明らかにした。. |
| 49. | 片倉 喜範, 山下万貴子, 原田額郎, 松本信英, 相葉佳洋, 市川明, 藤木司, 鵜殿美弥子, 樺山繁, 好田正, Pingbo Zhang, Hiroshi Fujii, 白畑 實隆, Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin, Immunobiology, 10.1016/j.imbio.2013.08.011, 219, 2, 149-157, 2014.02. |
| 50. | 片倉 喜範, Ahmed Ashour, Saleh El-Sharkawy, Mohamed Amer, Fatma Abdel Bar, Tomofumi Miyamoto, Nozomi Toyota, Tran Hai Ban, RYUICHIRO KONDO, kuniyoshi shimizu, Rational design and synthesis of topoisomerase I and II inhibitor based on olenanolic acid moiety for new anti-cancer drugs, Bioorg. Med. Chem., 22, 211-220, 2014.01. |
| 51. | 片倉 喜範, Tran Hai Band, Hiroto Suhara, Katsumi DOI, HIROYA ISHIKAWA, Katsuya Fukami, Gopal Prasad Parajuli, 山下俊太郎, Kazuo Watanabe, Mahesh Kumar Adhikari, Hira Kaji Manadhar, RYUICHIRO KONDO, Kuniyoshi Shimizu, Wild Mushroom in Nepal: Some potential Candidates as Antioxidant and ACE Inhibition Sources, Evid. Based Complement Alternat Med., 10.1155/2014/19305, 2014, 2014.01. |
| 52. | 片倉 喜範, 富松航佑, 松本信英, 田中勇人, 山下万貴子, 中西秀和, Kiichiro Teruya, Saiko Kazuno, 金城智也, 濱崎武記, 楠本賢一, 樺山繁, 白畑 實隆, A rapid screening and production method using a novel mammalian cell display to isolate human monoclonal antibodies., Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2013.10.007, 441, 1, 59-64, 2013.10. |
| 53. | M. Tanikawa, O. Wada-Hiraike, N. Yoshizawa-Sugata, A. Shirane, M. Hirano, H. Hiraike, Y. Miyamoto, K. Sone, Y. Ikeda, T. Kashiyama, K. Oda, K. Kawana, Y. Katakura, T. Yano, H. Masai, A. L Roy, Y. Osuga, . T. Fujii, Role of multifunctional transcription factor TFII-I and putative tumor suppressor DBC1 in cell cycle and DNA double strand damage repair., Br. J. Cancer, 109, 3042-3048, 2013.07. |
| 54. | 片倉 喜範, Gakuro Harada, Shin-ei Matsumoto, Makiko Yamashita, Kaoru Fujii, 白畑 實隆, In vitro immunization of Epstein-Barr virus-immortalized B cells augments antigen-specific antibody production, Cytotechnolgoy, 10.1007/s10616-013-9596-9, 65, 979-983, 2013.06. |
| 55. | 片倉 喜範, KOHJI YAMAMOTO, Hirofumi Ichinose, Yoichi Aso, 鵜殿美弥子, Upregulation of cytochrome P450s following exposure of the silkmoth, Bobyx mori to insecticides, J. Insect Biotechnol. Sericology, 82, 33-38, 2013.06. |
| 56. | 片倉 喜範, 小熊一郎, 山下俊太郎, 佐藤聡, 奧山和雄, Novel purification method of human IgG by using a thermo-responsive protein A, J. Chromatogr. A , 10.1016/j.chroma.2013.07.015, 1305, 149-153, 2013.04, 抗体精製のために用いられるProtein Aに変わる、新たな温度感受性のProtein Aの開発に関わる研究。. |
| 57. | Yoshinori Katakura, Yu-Jin, Ko, Hui-Hun, Kim, Eun-Jung, Kim, Won-Sup, Lee, Gon-Sup, Kim, Chung-Ho, Ryu, Piceatannol inhibits mast cell-mediated allergic inflammation, Int. J. Mol. Med., 2012.11. |
| 58. | Yoshinori Katakura, Miyako Udono, Keishi Kadooka, Shuntaro Yamashita, Quantitative analysis of cellular senescence phenotypes using an imaging cytometer, Methods, 56, 383-388, 56: 383-388, 2012.05. |
| 59. | Makiko Yamashita, Akira Hitaka, Himiko Fujino, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu, Tsukasa Fujiki, Yoshinori Katakura, Identification of immunopotentiating lactic acid bacteria that induce antibody production by in vitro-stimulated human peripheral blood mononuclear cells, Bioscience of Microbiota, Food and Health, 31:7-13, 2012.01. |
| 60. | Shuntaro Yamashita, Kaori Ogawa, Takahiro Ikei, Miyako Udono, Tsukasa Fujiki, Yoshinori Katakura, SIRT1 prevents replicative senescence of normal human umbilical cord fibroblast through potentiating the transcription of human telomerase reverse transcriptase gene, Biochem. Biophys. Res. Commun., 417: 630-634, 2012.01. |
| 61. | K. Yamamoto, Y. Tsuji, Y. Aso, T. Hamasaki, S. Shirahata, Y. Katakura, Effect of diazinon exposure on antioxidant reactions in the silkmoth, Bombyx mori, J. Appl. Entomol., 2011.05. |
| 62. | Koji Yamamoto, S. Teshiba, Y. Shigeoka, Yoichi Aso, Yutaka Banno, Tsukasa Fujiki, Yoshinori Katakura, Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth, Insect Mol. Biol., 2011.05. |
| 63. | Keishi Kadooka, Asuka Imahayashi, Ayaka Koiso, Makiko Yamashita, Kiichiro Teruya, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu, Yoshinori Katakura, Establishment of a novel method of screening anti-allergic lactic acid bacteria, Biosci. Biotechnol. Biochem.,, 75:1016-1018, 2011.05. |
| 64. | Li-Tao Tong, Yoshinori Katakura, Sayaka Kawamura, Sanae Baba, Yasutake Tanaka, Miyako Udono, Yoshie Kondo, Kumu Nakamura, Katsumi Imaizumi, Masao Sato, Effects of Kurozu concentrated liquid on adipocyte size in rats, Lipids in Health and Disease, 9, 134, 2010.09. |
| 65. | Tsukasa Fujiki, Miyako Udono, Yojiro Kotake, Makiko Yamashita, Sanetaka Shirahata, Yoshinori Katakura, NFAT5 regulates transcription of the mouse telomerase reverse transcriptase gene, Exp. Cell Res., 316, 3342-3350, 2010.05. |
| 66. | Tsukasa Fujiki, Atsuji Tsuji, Shin-ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Sanetaka Shirahata, Yoshinori Katakura, Generation of human anti-tumor necrosis factor-α monoclonal antibody by in vitro immunization with a multiple antigen peptide, Biosci. Biotechnol. Biochem., 74, 1836-1840, 2010.05. |
| 67. | Tsukasa Fujiki, Miyako Udono, Takumi Miura, Santaka Shirahata, Yoshinori Katakura, Regulatory mechanisms of human and mouse telomerase reverse transcriptase gene transcription: distinct dependency on c-Myc, Cytotechnology, 10.1007/s10616-010-9276-y, 2010.04. |
| 68. | Kosuke Tomimatsu, Shin-ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Yoshinori Katakura, Shigeru Kabayama, Sanetaka Shirahata, Production of human monoclonal antibodies against FcεRIα by a method combining in vitro immunization with phage display, Biosci. Biotechnol. Biochem., 73, 7, 1465-1469, 2009.07. |
| 69. | Yoshinori Katakura, Miyako Udono, Kazuyuki Katsuki, Hisaya Nishide, Yukiko Tabira, Takahiro Ikei, Makiko Yamashita, Tsukasa Fujiki, Sanetaka Shirahata, Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells, J. Biochem, 146, 1, 87-93, 2009.04. |
| 70. | Kaichi Yoshizaki, Tsukasa Fujiki, Takahiro Tsunematsu, Makiko Yamashita, Miyako Udono, Sanetaka Shirahata, Yoshinori Katakura, Pro-senescent effect of hydrogen peroxide on cancer cells and its possible application to tumor suppuression, Biosci. Biotechnol. Biochem., 73, 2, 311-315, 2009.02. |
| 71. | Yeon Suk Jung, Shin-ei Matsumoto, Yoshinori Katakura, Makiko Yamashita, Kosuke Tomimatsu, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata, Generation of human monoclonal antibodies against Propionibacterium acnes by applying the phage display method to human peripheral blood mononuclear cells immunized in vitro, Cytotechnol., 57: 169-175, 2008.09. |
| 72. | Miyako Udono, Tsukasa Fujiki, Makiko Yamashita, Kazuyuki Katsuki, Masaaki Funata Yusuke Imada, Kaichi Yoshizaki, Sanetaka Shirahata, Yoshinori Katakura, Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter, Biosci. Biotechnol. Biochem., 72: 1638-1641, 2008.05. |
| 73. | Shin-ei Matsumoto, Makiko Yamashita, Yoshinori Katakura¶, Yoshihiro Aiba, Kosuke Tomimatsu, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata, A rapid and efficient strategy to generate antigen-specific human monoclonal antibody by in vitro immunization and the phage display method, J. Immunol. Methods, 332, 2-9, 2008.02. |
| 74. | Jun Ye, Yuping Li, Takeki, Hamasaki, Noboru Nakamichi, Takeshi Kawahara, Kazuhiro Osada, Kiichiro Teruya, Yuko Kato, Kazuko Toh, Masumi Abe, Yoshinori Katakura, Sanetaka Shirahata¶, Catalyzer-21TM, a mineral water derived from leaf soil, inhibits tumor cell invasion and angiogenesis, Cytotechnol., 55, 61-70, 2008.02. |
| 75. | Jun Ye, Yuping Li, Takeki Hamasaki, Noboru Nakamichi, Takaaki Komatsu, Taichi Kashiwagi, Kiichiro Teruya, Ryuhei Nishikawa, Takeshi Kawahara, Kazuhiro Osada, Kazuko Toh, Masumi Abe, Huaize Tian, Shigeru Kabayama, Kazumichi Otsubo, Shinkatsu Morisawa, Yoshinori Katakura, Sanetaka Shirahata¶, Inhibitory effect of electrolyzed reduced water on tumor angiogenesis, Biol. Pharm. Bull., 31, 19-26, 2008.02. |
| 76. | Yeon Suk Jung, Shin-ei Matsumoto, Makiko Yamashita, Kosuke Tomimatsu, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata, Propionibacterium acnes acts as an adjuvant in in vitro immunization of human peripheral blood mononuclear cells, Biosci. Biotechnol. Biochem., 71: 1963-1969, 2007.05. |
| 77. | Makiko Yamashita¶, Yoshinori Katakura, Yoshihiro Aiba, Shin-ei Matsumoto, Kazuko Morihara, Kiichiro Teruya, Akira Ichikawa, Sanetaka Shirahata, Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells, Cytotechnol., 55, 71-77, 2007.05. |
| 78. | Makiko Yamashita¶, Yoshinori Katakura, Sanetaka Shirahata, Recent advances in the generation of human monoclonal antibody, Cytotechnol., 55, 55-60, 2007.05. |
| 79. | Takashi Tamura, Kosuke Tomimatsu, Yoshinori Katakura, Makiko Yamashita¶, Shin-ei Matsumoto, Yoshihiro Aiba, Yeon Suk Jung, Yoshiichi Abe, Tsukasa Fujiki, Kiichiro Teruya, Sanetaka Shirahata, Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells, Biosci. Biotechnol. Biochem., 71, 2871-2875, 2007.05. |
| 80. | Tsukasa Fujiki, Takumi Miura, Mariko Maura, Hiroshi Shiraishi, Yusuke Imada, Norihisa Uehara, Kosuke Tashiro, Sanetaka Shirahata, Yoshinori Katakura, TAK1 represses transcription of the human telomerase reverse transcriptase gene, Oncogene, in press, 2007.03. |
| 81. | Jun Ye, Kiichiro Teruya, Yoshinori Katakura, Hiroshi Eto and Sanekata Shirahata, Suppressive effect of fucoidan derived from Mozuku on in vitro invasion of human fibrosarcoma HT1080 cells, Animal Cell Technology: Basic & Applied Aspects, pp. 409-415, 2006.04. |
| 82. | Ryuhei Nishikawa, Kiichiro Teruya, Yoshinori Katakura, Kazumichi Otsubo, Shinkatsu Morisawa, Qianghua Xu and Sanetaka Shirahata, Suppression of two-stage cell transformation by electrolyzed reduced water containing platinum nanoparticles, Animal Cell Technology: Basic & Applied Aspects, pp.113-119, 2006.04. |
| 83. | Shin-ei Matsumoto, Makiko Yamashita, Yoshinori Katakura, Eri Noguchi, Yoshihiro Aiba, Akira Ichikawa, Kiichiro Teruya, Sanetaka Shirahata, In vitro immunization can elicit the expansion of diverse repertoire of B cells from peripheral blood mononuclear cells, Cytotechnol., 52: 227-233, 2006.04. |
| 84. | Yoshihiro Aiba, Makiko Yamashita, Yoshinori Katakura, Yuki Furukawa, Shin-ei Matsumoto, Kousuke Tomimatsu, Kiichiro Teruya, and Sanetaka Shirahata, Identification of genes involved in the suppression of antibody production from human peripheral blood lymphocytes, Biosci. Biotechnol. Biochem., 70：966-970, 2006.04. |
| 85. | Yoshinori Katakura, Molecular basis for the cellular senescence program and its application to anticancer therapy, Biosci. Biotechnol. Biochem., 79: 1076-1081, 2006.04. |
| 86. | Yoshinori Katakura, Kaichi Yoshizaki, Tomoharu Yasuda, Takahiro Tsunematsu, Norihisa Uehara, Takumi Miura, Tsukasa Fujiki and Sanetaka Shirahata, Functional impairment of telomerase in sublines derived from human lung adenocarcinoma exposed to mild oxidative stress, Biochem. Biophys. Res. Commun, 10.1016/j.bbrc.2005.06.109, 334, 2, 450-459, 334, 450-459, 2005.01. |
| 87. | Shunsuke Fukumoto, Masayo Morifuji, Yoshinori Katakura, Masamichi Ohishi, Seiji Nakamura, Endostatin inhibits lymph node metastasis by a down-regulation of the vascular endothelial growth factor C expression in tumor cells, Clin. Exp. Metastasis, 10.1007/s10585-005-3973-5, 22, 1, 31-38, 22, 31-38, 2005.01. |
| 88. | Ryuhei Nishikawa, Kiichiro Teruya, Yoshinori Katakura, Kazuhiro Osada, Takeki Hamasaki, Taichi Kashiwagi, Takaaki Komatsu, Yuping Li, Jun Ye, Akira Ichikawa, Kazumichi Otsubo, Shinkatsu Morisawa and Sanetaka Shirahata, Electrolyzed reduced water supplemented with platinum nanoparticles suppresses promotion of two-stage cell transformation, Cytotechnol., 10.1007/s10616-005-3759-2, 47, 1-3, 97-105, 47: 97-105, 2005.01. |
| 89. | Jun Ye, Yuping Li, Kiichiro Teruya, Yoshinori Katakura, Akira Ichikawa, Hiroshi Eto, Mutsutaka Hosoi, Masako Hosoi, Shinji Nishimoto and Sanetaka Shirahata, Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells, Cytotechnol., 10.1007/s10616-005-3761-8, 47, 1-3, 117-126, 47: 117-126, 2005.01. |
| 90. | Qianghua Xu, Yoshinori Katakura, Makiko Yamashita, Shengguo Feng, Takashi Tamura, Shin-ei Matsumoto, Yoshihiro Aiba, Kiichiro Teruya, Kazuhiro Osada, Ryuhei Nishikawa, Sanetaka Shirahata, IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation, Biosci. Biotechnol. Biochem., 10.1271/bbb.68.2279, 68, 11, 2279-2284, 68, 2279-2284, 2004.01. |
| 91. | Kenichi Yamaguchi, Mizuki Uechi, Yoshinori Katakura, Tatsuya Oda, and Masatsune Ishiguro, Mitogenic Properties of Pokeweek Lectin-D Isoforms on Human Peripheral Blood Lymphocytes: Non-mitogen PL-D1 and Mitogen PL-D2, Biosci. Biotechnol. Biochem., 10.1271/bbb.68.1591, 68, 7, 1591-1593, 68: 1591-1593, 2004.01. |
| 92. | Yoshinori Katakura, Molecular basis for regulating cellular senescence program and its possible application to drug design, Nippon Nogeikagaku Kaishi-J. JSBBA., 78, 12, 1142-1147, 78, 1142-1147, 2004.01. |
| 93. | Yoshinori Katakura, Eriko Nakata, Yukiko Tabira, Takumi Miura, Kiichiro Teruya, Toshie Tsuchiya and Sanetaka Shirahata, Decreased tumorigenicity in vivo when transforming growth factor beta treatment causes cancer cell senescence, Biosci. Biotechnol. Biochem., 67, 4, 815-821, 67: 815-823, 2003.01. |
| 94. | Akio Ametani, Toshio Sakurai, Yoshinori Katakura, Satoru Kuhara, Hideki Hirakawa, Tomohiro Hosoi, Shunichi Dosako, Shuichi Kaminogawa, Amino acid residue substitution at T-cell determinant-flanking sites in b-lactoglobulin modulates antigen presentation to T cells through subtle conformational change, Biosci. Biotechnol. Biochem., 67, 7, 1507-1514, 67: 1507-1514, 2003.01. |
| 95. | Xioyan Dong, Kiichiro Teruya, Yoshinori Katakura, Yingpei Zhang, Takumi Miura, Yoshihito Daimon, T. Mori, H. Ohashi and Sanetaka Shirahata, A hybrid system using both promoter activation and gene amplification for establishing exogenous protein hyper-producing cell lines, Cytotechnol., 10.1023/B:CYTO.0000039901.92984.7a, 43, 1-3, 11-17, 43: 11-17, 2003.01. |
| 96. | Tsutomu Nagira, Junko Narisawa, Kiichirou Teruya, Yoshinori Katakura, Sun Yup Shim, Ken ichi Kusumoto, Sennosuke Tokumaru, Koichiro Tokumaru, David W. Barnes, Sanetaka Shirahata, Suppression of UVC-induced cell damage and enhancement of DNA repair by the fermented milk, Kefir, Cytotechnology, 10.1023/A:1023984304610, 40, 1-3, 125-137, 2002.11, [URL], An aqueous extract of Kefir, fermented milk originally produced in the Caucasus mountains, suppressed morphological changes of human melanoma HMV-1 and SK-MEL cells and human normal fibroblast TIG-1 cells caused by UVC-irradiation, suggesting that UV damage can be suppressed by the Kefir extract. The addition of the Kefir extract after UVC-irradiation of HVM-1 cells resulted in a remarkable decrease in intracellular reactive oxygen species (ROS) which had been increased by UVC irradiation. The Kefir extract also stimulated unscheduled DNA synthesis and suppressed UVC-induced apoptosis of HMV-1 cells. A colony formation assay revealed that the Kefir extract rescued HMV-1 cells from cell death caused by UVC irradiation. The Kefir extract, as well as methyl methanethiosulfonate which is known to enhance the nucleotide excision repair (NER) activity, exhibited strong thymine dimer repair-enhancing activity. Epigalocatechin exhibited a weak NER activity but vitamins A, C, and E and catechin showed no NER activity. The thymine dimer repair-enhancing factors in the Kefir extract were heatstable and assumed to be molecules with a molecular weight of less than 5000. The treatment of HMV-1 cells with the Kefir extract during or before UVC-irradiation also prevented the generation of ROS and thymine dimmer, and suppressed the apoptosis of HMV-1 cells, suggesting that application of Kefir can prevent UV damage.. |
| 97. | Sum Yup Shim, Akira Ichikawa, Makiko Yamashita, Yoshinori Katakura, Kiichiro Teruya, Yasutaka Mochizuki, Emi Tobinaga, Sanetaka Shirahata, Construction of an in vitro allergy reaction evaluation system using human leukemia cell lines, Cytotechnol., 10.1023/A:1023918206060, 40, 1-3, 75-83, 40: 75-83, 2002.01. |
| 98. | Yuping Li, Tomohiro Nishimura, Kiichiro Teruya, Tei Maki, Takaaki Komatsu, Takeki Hamasaki, Taichi Kashiwagi, Shigeru Kabayama, Sum Yup Shim, Yoshinori Katakura, Kazumichi Otsubo, Shinkatsu Morisawa, Yoshitoki Ishii, Zbigniew Gadek, Sanetaka Shirahata, Protective mechanism of reduced water against alloxan-induced pancreatic b-cell damage: Scavenging effect against reactive oxygen species, Cytotechnol., 10.1023/A:1023936421448, 40, 1-3, 139-149, 40: 139-149, 2002.01. |
| 99. | Makiko Yamashita, Yoshinori Katakura, Sum Yup Shim, Shin-ei Matsumoto, Takashi Tamura, Kazuko Morihara, Yoshihiro Aiba, Kiichiro Teruya, Toshie Tsuchiya, Sanetaka Shirahata, Different individual immune response elicited by in vitro immunization, Cytotechnol., 10.1023/A:1023992506427, 40, 1-3, 161-165, 40: 161-165, 2002.01. |
| 100. | Kiichiro Teruya, Zhang Yingpei, Yoshinori Katakura, Sanetaka Shirahata, A regulatable selective system facilitates isolation of hetelologous protein hyper-producing mammalian cells without gene amplification, Cytotechnol., 10.1023/A:1023945517446, 40, 1-3, 13-22, 40: 13-22, 2002.01. |
| 101. | Tsunomu Nagira, Junko Narisawa, Kiichiro Teruya, Yoshinori Katakura, Sennosuke Tokumaru, Koichiro Tokumaru, David Barnes, Sanetaka Shirahata, Protection from UV damage in animal cells by Kefir extract, Cytotechnol., 40: 125-137, 2002.01. |
| 102. | Kiichiro Teruya, Maiko Yamashita, Rumi Tominaga, Yoshinori Katakura, Sennosuke Tokumaru, David Barnes, Sanetaka Shirahata, Fermented milk, Kefran-Kefir enhances glucose uptake into insulin-responsive muscle cells, Cytotechnol., 10.1023/A:1023926407877, 40, 1-3, 107-116, 40: 107-116, 2002.01. |
| 103. | Makiko Yamashita, Akira Ichikawa, Yoshinori Katakura, Yasutaka Mochizuki, Kiichiro Teruya, E. _H. Kim and Sanetaka Shirahata, Induction of basophilic and eosinophilic differentiation in the human leukemic cell line KU812, Cytotechnol., 36: 084-193, 2001.01. |
| 104. | Takeshi Kawahara, Akira Ichikawa, Yoshinori Katakura, Kiichiro Teruya, Takahisa Yoshida, M. Kikuchi, M. Kamei, Shuichi Hashizume and Sanetaka Shirahata, Establishment of hybridomas producing cancer-specific human antibodies from B cell line derived from PBL of a patient with adult T cell leukemia, Cytotechnol., 10.1023/A:1014049205434, 36, 1-3, 171-177, 36: 176-183, 2001.01. |
| 105. | Takumi Miura, Yoshinori Katakura, Katsuhiko Yamamoto, Norihisa Uehara, Toshie Tsuchiya, E. -H. Kim and Sanetaka Shirahata, Neural stem cells lose telomerase activity upon differentiation into astrocytes, Cytotechnol., 10.1023/A:1014016315003, 36, 1-3, 137-144, 36: 140-149, 2001.01. |
| 106. | S. -Y. Shim, Yoshinori Katakura, Akira Ichikawa, Kiichiro Teruya, Tsukasa Matsuda and Sanetaka Shirahata, Epitope analysis of human monoclonal antibody specific for rice allergenic protein generated by in vitro immunization, Cytotechnol., 10.1023/A:1014012214095, 36, 1-3, 109-115, 36: 108-115, 2001.01. |
| 107. | Akira Ichikawa, S. _Y. Shim, Yoshinori Katakura, Kiichiro Teruya and Sanetaka Shirahata, Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8, Cytotechnol., 10.1023/A:1014060113186, 36, 1-3, 101-107, 36: 100-107, 2001.01. |
| 108. | Norihisa Uehara, Yoshinori Katakura, Takumi Miura, Sanetaka Shirahata, Subtractive screening of genes involved in cellular senescence, Cytotechnol., 10.1023/A:1008103230335, 35, 1, 35-42, 35: 35-42, 2001.01. |
| 109. | Takumi Miura, Yoshinori Katakura, Perry Seto, Yingpei Zhang, Kiichiro Teruya, Eisaku Nishimura, Masatoshi Kato, Shuichi Hashizume, Sanetaka Shirahata, Availability of oncogene activated production system for mass production of light chain of human antibody in CHO cells, Cytotechnol., 10.1023/A:1008179919857, 35, 1, 9-16, 35: 9-16, 2001.01. |
| 110. | Seiji Kawamoto, Naoko Iemura, Yuichi Inoue, Yoshinori Katakura, Sanetaka Shirahata, Effect of 14-3-3 protein induction on cell proliferation of A549 human lung adenocarcinoma, Cytotechnol., 10.1023/A:1008129020728, 33, 1-3, 253-257, 33: 253-257, 2000.01. |
| 111. | Kazunari Kanegawa, Hideki Harada, Hisashi Myouga, Yoshinori Katakura, Sanetaka Shirahata, Telomerase inhibiting activity in vitro from natural resources, marine algae extracts, Cytotechnol., 10.1023/A:1008179302906, 33, 1-3, 221-227, 33: 221-227, 2000.01. |
| 112. | Shahabuddin Alam, Yoshinori Katakura, Hiroshi Yoshida, Eun-Ho Kim, Sanetaka Shirahata, Improvement of a method to reproducibly immortalize human T cells by oncogene transfection, Cytotechnol., 10.1023/A:1008171109981, 33, 1-3, 71-81, 33: 71-78, 2000.01. |
| 113. | Yuichi Inoue, Mihoko Fujisawa, Masahiro Shoji, Shuichi Hashizume, Yoshinori Katakura, Sanetaka Shirahata, Enhanced antibody production of human-human hybridomas by retinoic acid, Cytotechnol., 10.1023/A:1008155609072, 33, 1-3, 83-88, 33: 83-88, 2000.01. |
| 114. | Yuichi Inoue, Seiji Kawamoto, Masahiro Shoji, Shuichi Hashizume, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata, Properties of ras-amplified recombinant BHK-21 cells in protein-free culture, Cytotechnol., 10.1023/A:1008163312841, 33, 1-3, 21-26, 33: 21-26, 2000.01. |
| 115. | Yoshinori Katakura, Eriko Nakata, Takumi Miura and Sanetaka Shirahata, Transforming growth factor beta triggers two independent-senescence programs in cancer cells, Biochem. Biophys. Res. Commun, 10.1006/bbrc.1999.0129, 255, 1, 110-115, 255: 110-115, 1999.01. |
| 116. | Yoshinori Katakura, Akio Ametani, Mamoru Totsuka, Shin-ya Nagafuchi and Shuichi Kaminogawa, Accelerated secretion of mutant beta-lactoglobulin in Saccharomyces cerevisiae resulting from a single amino acid substitution, Biochim. Biophys. Acta, 10.1016/S0167-4838(99)00099-0, 1432, 2, 302-312, 1432: 302-312, 1999.01. |
| 117. | Yuichi Inoue, Nobuhisa Arita, Kiichiro Teruya, Yoshinori Katakura and Sanetaka Shirahata, Efficient production of recombinant human erythropoietin by replenishment of microcarriers into the hollow fiber culture cassette, Biosci. Biotech. Biochem.,, 10.1271/bbb.63.1624, 63, 9, 1624-1626, 63: 16247-1626, 1999.01. |
| 118. | Shahabuddin Alam, Yoshinori Katakura and Sanetaka Shirahata, Establishment of human T cell clones exhibiting natural killer-like activity, Cytotechnol., 31, 1-2, 171-176, 31: 171-176, 1999.01. |
| 119. | Akira Ichikawa, Yoshinori Katakura, Kiichiro Teruya, Shuichi Hashizume and Sanetaka Shirahata, In vitro immunization of human peripheral blood lymphocytes: establishment of B cell lines secreting IgM specific for cholera toxin B subunit from lymphocytes stimulated with IL-2 and IL-4, Cytotechnol., 31, 1-2, 131-139, 31: 131-139, 1999.01. |
| 120. | Yuichi Inoue, Mihoko Fujisawa, Seiji Kawamoto, M. Shoji, Shuichi Hashizume, M. Fujii, Yoshinori Katakura and Sanetaka Shirahata, Effectiveness of vitamin A acetate for enhancing the production of lung cancer specific monoclonal antibodies, Cytotechnol., 10.1023/A:1008016020785, 31, 1-2, 77-83, 31: 77-83, 1999.01. |
| 121. | Yoshinori Katakura, Perry Seto, Takumi Miura, Hideya Ohashi, Kiichiro Teruya and Sanetaka Shirahata, Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes, Cytotechnol., 10.1023/A:1008048928053, 31, 1-2, 103-109, 31: 103-109, 1999.01. |
| 122. | Kenji Enpuku, Tadashi Minotani, Takemitsu Gima, Yukinori Kuroki, Yuzuru Itoh, Makiko Yamashita, Yoshinori Katakura and Satoru Kuhara, Detection of Magnetic Nanoparticles with Superconducting Quantum Interference Device (SQUID) Magnetometer and Application to Immunoassays, Jpn. J. Appl. Phys., 10.1143/JJAP.38.L1102, 38, 10A, L1102-L1105, 38: 1102-1105, 1999.01. |
| 123. | Sanetaka Shirahata, Yoshinori Katakura, Kiichiro Teruya, Cell hybridization, hybridomas, and human hybridomas, Methods in Cell Biology, 57, 111-145, 1998.12. |
| 124. | 片倉喜範、Shahabuddin Alam、白畑實隆, Immortalization by gene transfection, Meth. Cell Biol., 57, 69-91, 1998.10. |
| 125. | Thongpassano Siripen, Hirofumi Tachibana, Yoshinori Katakura, Sanetaka Shirahata, Improvement of antigen binding ability of human antibodies by light chain shifting, Cytotechnology, 25, 1-3, 155-164, 1997.12, Human HB4C5 hybridoma cells produce a lung cancer-specific IgM human monoclonal antibody (mAb). HB4C5 human mAb cross-reacts with Candida cytochrome c (Cyt c) and carboxypeptidase (Cpase). Concanavalin A (ConA)-resistant variants of HB4C5 cells loss the original light chain followed by expression of various new light chains at a high incidence (light chain shifting) (Tachibana et al., 1996). HTD8 cells, one of the ConA-resistant variant subclones of HB4C5 cells, undergo the active light chain shifting and produce various sublines, each of which stably secretes new mAb consisting of a new light chain and a HB4C5 heavy chain. The new mAb exhibits altered antigen binding ability from that of the original antibody. We could expect that HTD8 cells can be used as 'a light chain stem cell line' to improve antigen binding ability and specificity of established human mAbs. A BD9D12 IgG human mAb recognizes lung cancer cells and cross-reacts with cytokeratin 8. Introduction of the heavy chain gene of BD9D12 mAb into HTD8 cells resulted in establishment of various sublines which secreted various kinds of hybrid antibodies consisting of different light chains derived from HTD8 subclones which underwent light chain shifting and a common IgG heavy chain derived from BD9D12. These hybrid antibodies exhibited different or improved reactivities to Cyt, Cpase, cytokeratin 8 and various cancer cells from those of parental mAb, demonstrating that light chain shifting can be applied to improve the affinity and specificity of human mAb.. |
| 126. | Yingpei Zhang, Yoshinori Katakura, Hideya Ohashi, Sanetaka Shirahata, Efficient and inducible production of human interleukin 6 in Chinese hamster ovary cells using a novel expression system, Cytotechnology, 25, 1-3, 53-60, 1997.12, High level and inducible production of human interleukin 6 (hIL-6) was achieved using a novel expression system in Chinese hamster ovary (CHO) cells. In this system, the transcription of hIL-6 gene under the control of PhCMV*-1 promoter composed of tetracycline operator sequences and a minimal promoter is activated by a chimeric transactivator (tTA) composed of tetracycline repressor and transactivating domain of VP16 protein of herpes simplex virus. The transcription of tTA gene, which is also under the control of PhCMV*-1 promoter, is activated by itself via a positive feedback cycle. The expression of both genes is further enhanced by potentiating the VP16 transactivating domain of tTA transactivator with pX protein of hepatitis B virus. In the presence of tetracycline, the tTA transactivators can not bind to PhCMV*-1 promoter, therefore, the expression of hIL-6 and tTA gene is suppressed, and the pX will not activate basal transcription. In the absence of tetracycline, tTA transactivators bind to PhCMV*-1 promoter and activate efficient transcription of hIL-6 and tTA gene, and the transcription is further enhanced by pX via VP16 transactivating domain. Using this strategy, we isolated a clone (UX1) producing hIL-6 at a rate about 1425 ng/106 cells/day. Furthermore, the hIL-6 production is stringently regulated by tetracycline. This results suggested a novel strategy to establish highly efficient, inducible and cell type independent recombinant protein production system by using an artificial promoter to recruit transactivators and coactivators which can synergistically activate transcription.. |
| 127. | Yingpei Zhang, Yoshinori Katakura, Hideya Ohashi, Sanetaka Shirahata, An autocatalytic expression system for regulated production of recombinant protein in mammalian cells, Analytical Biochemistry, 10.1006/abio.1997.2325, 252, 2, 286-292, 1997.10, [URL], An autocatalytic inducible mammalian expression system was established. This system is composed of two sets of vectors: one carries a selectable marker gene and the other carries a bicistronic expression unit consisting of a target gene, an internal ribosomal entry site, and a tetracycline- controlled transactivator gene. Both the selectable marker and the bicistronic unit are controlled by the tetracycline-responsive promoter (PhCMV(*)-1). When the two vectors are cotransfected into host cells, only a single selection round in the absence of tetracycline is necessary to generate clones expressing the target gene. The expression level is high and easily regulated by tetracycline. Combination with a gene amplification system may allow further enhance the foreign gene expression. Because the PhCMV-1 promoter is relatively independent of cellular regulation signals, this system is expected to work in a wide variety of cell types.. |
| 128. | 片倉喜範、山本克彦、＃三宅修、＃保田朋波流、＃上原範久、＃中田恵理子、＃河本正次、白畑實隆, Bi-directional regulation of telomerase activity in a subline derived from human lung adenocarcinoma, Biochem. Biophys. Res. Commun., 237, 313-317, 1997.05. |
| 129. | Sanetaka Shirahata, Shigeru Kabayama, Mariko Nakano, Takumi Miura, Kenichi Kusumoto, Miho Gotoh, Hidemitsu Hayashi, Kazumichi Otsubo, Shinkatsu Morisawa, Yoshinori Katakura, Electrolyzed-reduced water scavenges active oxygen species and protects DNA from oxidative damage, Biochemical and Biophysical Research Communications, 10.1006/bbrc.1997.6622, 234, 1, 269-274, 1997.05, [URL], Active oxygen species or free radicals are considered to cause extensive oxidative damage to biological macromolecules, which brings about a variety of diseases as well as aging. The ideal scavenger for active oxygen should be 'active hydrogen'. 'Active hydrogen' can be produced in reduced water near the cathode during electrolysis of water. Reduced water exhibits high pH, low dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and extremely negative redox potential (RP) values. Strongly electrolyzed-reduced water, as well as ascorbic acid, (+)-catechin and tannic acid, completely scavenged O 2 ̇ - produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium phosphate buffer (pH 7.0). The superoxide dismutase (SOD)-like activity of reduced water is stable at 4°C for over a month and was not lost even after neutralization, repeated freezing and melting, deflation with sonication, vigorous mixing, boiling, repeated filtration, or closed autoclaving, but was lost by opened autoclaving or by closed autoclaving in the presence of tungsten trioxide which efficiently adsorbs active atomic hydrogen. Water bubbled with hydrogen gas exhibited low DO, extremely high DH and extremely low RP values, as does reduced water, but it has no SOD-like activity. These results suggest that the SOD-like activity of reduced water is not due to the dissolved molecular hydrogen but due to the dissolved atomic hydrogen (active hydrogen). Although SOD accumulated H 2 O 2 when added to the HX-XOD system, reduced water decreased the amount of H 2 O 2 produced by XOD. Reduced water, as well as catalase ascorbic acid, could directly scavenge H 2 O 2 . Reduced water suppresses single-strand breakage of DNA by active oxygen species produced by the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner, suggesting that reduced water can scavenge not only O 2 ̇ - and H 2 O 2 , but also 1 O 2 and . OH.. |
| 130. | Shahabuddin Alam, Yoshinori Katakura, Hiroshi Yoshida, Eun Ho Kim, Sanetaka Shirahata, Functional characterization of human T cells immortalized by oncogene transfection, Cytotechnology, 10.1023/a:1007928021139, 23, 1-3, 185-192, 1997.01, [URL], We have succeeded in immortalizing human lymphocytes derived from the peripheral blood of a healthy donor and of an atopic patient, and from the lymph node of a cancer patient by oncogene transfection (Alam et al., 1996). All immortalized human lymphocytes were shown to be CD3+ and CD19-, indicating that these immortalized human lymphocytes were all T cells. We established 317, 154 and 692 individual immortalized human T cell lines derived from the healthy donor, the atopic patient and the cancer patient, respectively. The ratios of CD4+ and CD8+ subpopulations within the set containing immortalized T cells derived from the healthy donor were shown to be varied depending on the combinations of transfected oncogenes used. However, CD8+ cells were found to be the dominant subpopulation of immortalized T cells derived from the atopic patient and the cancer patient. These immortalized T cells showed different proliferative responses in the presence of exogenous human IL-2 depending on their origin, and was consistent with the surface expression of the IL-2 receptor. Furthermore, the cytokine secretion patterns of these immortalized T cells stimulated with mitogen were investigated. The results showed that the immortalized T cells from the healthy donor is able to secrete various kinds of cytokines such as IL-2, IL-10, γ-IFN and GM-CSF. However, immortalized T cells from the cancer patient was shown to only secrete IL-2 and GM-CSF. These results suggest that depending on the origin, the immortalized T cells came from different subsets or from cells in different activated states. Mixed lymphocytes reactions demonstrated that these immortalized T cells are able to proliferate in the presence of allogenic or xenogenic stimulator cells, suggesting that they maintain the ability to recognize specific antigens on the stimulator cells and can proliferate even after the immortalization. Furthermore, immortalized T cells derived from the healthy donor and the cancer patient strongly responded to K562 cells, suggesting that MHC-nonrestricted killer T cells were also immortalized.. |
| 131. | Tadakazu Tamai, Kazunari Tsujimura, Sanetaka Shirahata, Hiroshi Oda, Tohru Noguchi, Riichi Kusuda, Nobuyuki Sato, Shoji Kimura, Yoshinori Katakura, Hiroki Murakami, Development of DNA diagnostic methods for the detection of new fish iridoviral diseases, Cytotechnology, 10.1023/a:1007966325278, 23, 1-3, 211-220, 1997, [URL], A new disease of epidemic proportions caused by fish viruses within the Iridoviridae family inflicts serious damage on red sea breams (Pagrus major) and striped jack (Caranx delicatissimus) populations grown in aquacultures in Japan. A partial segment of the fish iridoviral DNA was directly amplified using the polymerase chain reaction (PCR) with synthetic primers designed from well conserved nucleotide sequences between the frog virus 3 (Ranavirus) and the silkworm iridescent virus type 6. The deduced amino acid sequence from the nucleotide sequence of the PCR fragment demonstrates a high correlation with a partial sequence from the frog virus 3. Using the PCR method with specific primers, we could detect three of four different known types of fish iridoviruses in diseased fishes. To construct more reliable detection methods specific for this viral family, DNA fragments which can specifically hybridize with all of the four known iridoviridae viral DNAs were screened from the genomic library of one iridoviridae strain. The hybridization assay, using a specific fragment which contains regions which are highly homologous with a characterized partial sequence from the frog virus 3, proved to be a reliable diagnostic tool for fish iridoviral diseases.. |
| 132. | Yoshinori Katakura, Mamoru Totsuka, Akio Ametani, Shuichi Kaminogawa, A small variance in the antigenicity but not function of recombinant β-lactoglobulin purified from the culture supernatant of transformed yeast cells, Cytotechnology, 10.1023/a:1007977709348, 23, 1-3, 133-141, 1997.01, [URL], We purified recombinant bovine β-lactoglobulin (rβ-LG) from the culture supernatant of transformed yeast and investigated whether rβ-LG maintained the functional ability and antigenicity of native β-LG. Immunostaining following gel electrophoresis and reversed-phase high-performance liquid chromatography confirmed that rβ-LG was purified homogeneously. rβ-LG showed almost the same retinol-binding ability as native β-LG purified from bovine milk. However, affinities of two anti-β-LG monoclonal antibodies (mAbs) to rβ-LG were different from those to native β-LG, although three other mAbs bound these two proteins equally. Since our panel of five mAbs has been previously shown to be able to detect structural changes occurring in β-LG, this variance in antigenicity can be attributed to conformational differences between rβ-LG and native β-LG. Then, we studied which step in the production and purification procedure was responsible for altering the antigenicity of rβ-LG. Bovine milk native β-LG was added to several steps in this procedure and purified in the same manner as rβ-LG. The results suggested that incubation in the yeast culture had adverse effects on maintaining the antigenicity of this recombinant protein. We conclude from these results that even if no difference between the native and recombinant proteins can be detected by functional analysis, some subtle conformational change which can be distinguished by mAbs may be incorporated into the recombinant protein during its production and ultimately cause a different immune reaction in vivo.. |
| 133. | Shigeru Kabayama, Kazuhiro Osada, Hirofumi Tachibana, Yoshinori Katakura, Sanetaka Shirahata, Enhancing effects of food components on the production of interferon β from animal cells suppressed by stress hormones, Cytotechnology, 23, 1-3, 119-125, 1997, In today's 'modern' society, no one can escape from the stresses of daily life. Stress stimulates the secretion of stress hormones (e.g. cortisol or noradrenaline) which generally suppress the immune response system, thus rendering the body vulnerable to infectious diseases and cancer. Therefore finding anti-stress food components, which diminish and/or inhibit the stress related suppression of the immune response system would be helpful in maintaining and promoting the health of the human population. Here we established a screening system for anti-stress substances using the cultured human cell line MG-63. The production of interferon-β (IFN-β) by MG-63 cells super-induced by Poly (I): Poly (C) was shown to decrease in a dose dependent manner upon the addition of 0.01-10 μg/ml of cortisol or noradrenaline (NA). 1,2-Diacylglycerol (DG) was demonstrated to abrogate this suppression. Lipid from the fermented milk, kefir, also inhibited the influence of cortisol. Kefiran, a polysaccharide secreted from L. kafiranofasiens GKL-28 diminished the cortisol or NA influenced IFN-β production. But phosphatidylcholine had no significant effect in this system. These results suggest that DG, lipids from kefir and kefiran may be equated as anti-stress food component.. |
| 134. | Yoshinori Katakura, Establishment of an abalone digestive gland cell line secreting various glycosides in protein free culture, Cytotechnology, 24, 169-175, 1997. |
| 135. | Yingpei Zhang, Yoshinori Katakura, Perry Seto, Sanetaka Shirahata, Evidence that phosphatidylcholine-specific phospholipase C is a key molecule mediating insulin-induced enhancement of gene expression from human cytomegalovirus promoter in CHO cells, Cytotechnology, 23, 1-3, 193-196, 1997, The signal transduction from insulin to its receptors and Ras has been extensively studied, while little has been reported beyond these steps. We found that the expression of human interleukin 6 gene under the control of immediate early gene promoter of human cytomegalovirus was enhanced by insulin stimulation in Chinese hamster ovary cells. The induction effect of insulin was not significantly affected by inhibitors or activators of conventional protein kinase C, cAMP dependent protein kinase and phosphoinositide -3 kinase, however, pre-incubation of the cells with D609, a specific inhibitors of phosphatidylcholine-specific phospholipase C completely abolished the induction effect. These results clearly demonstrate that phosphatidylcholine-specific phospholipase C is a key molecule mediating insulin-induced enhancement of hIL-6 expression from the human cytomegalovirus promoter in Chinese hamster ovary cells and strongly suggest that it plays an important role in the insulin signaling pathways.. |
| 136. | Yoshiyuki Dan, Yoshinori Katakura, Akio Ametani, Shuichi Kaminogawa, Yoshihiro Asano, IL-3 augments TCR-mediated responses of type 2 CD4 T cells, Journal of Immunology, 156, 1, 27-34, 1996.01, A subset of type 2, but not type 1, CD4 T cell clones expresses IL-3R and can be stimulated by IL-3. Expression of IL-3R on these type 2 T cell clones is induced by TCR stimulation, and subsequent stimulation by IL-3 augmented the proliferation of and IL-4 production by these cells. This augmented response is inhibited by anti-IL-4 mAb, suggesting the involvement of IL-4 in this response. In place of TCR stimulation, treatment of these type 2 CD4 T cell clones with PMA rendered them responsive to further stimulation of proliferation by IL-3, indicating the cooperation between the IL-3R-elicited signals and PKC-mediated signals in stimulating proliferation. Although the augmentation of the TCR-mediated proliferative response by IL-3 was mainly due to the increased production of IL-4, we also demonstrated the presence of IL-4-independent mechanism mediating the response to IL-3. In situ, we found that splenic T cells could be induced to respond to IL-3 by TCR stimulation. Thus, IL-3 can stimulate a specific population of T cells and influence the immune response.. |
| 137. | Yuichi Inoue, Lourdes Bouzo Lopez, Seiji Kawamoto, Nobuhisa Arita, Kiichiro Teruya, Kiyohiko Seki, Masahiro Shoji, Masanori Kamei, Shuichi Hashizume, Yasumitsu Shiozawa, Hirofumi Tachibana, Hideya Ohashi, Kosei Yasumoto, Akira Nagashima, Hisashi Nakahashi, Tatsuo Suzuki, Tadaaki Imai, Kikuo Nomoto, Mitsuhiro Takenoyama, Yoshinori Katakura, Sanetaka Shirahata, Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.60.811, 60, 5, 811-817, 1996.01, [URL], A ras oncogene-amplified recombinant BHK-21 cell line (ras-rBHK-IgG) has been established, and was shown to hyperproduce the recombinant IgG chimeric human monoclonal antibody (hMAb) AE6F4, which recognizes lung cancer cells. We found that the ras-rBHK-IgG cells could be easily cultured in a protein-free ERDF medium supplemented with iron(III) nitrate, hydroxyethyliminodiacetic acid, and non-protein synthetic attachment factor as well as in a serum-free ERDF medium supplemented with insulin, transferrin, ethanolamine, and sodium selenite. The productivity of recombinant hMAb from the cells cultured in dishes at high cell densities was higher in protein-free medium than in serum-containing medium.True high density culture of the ras-rBHK-IgG cells was done in protein-free medium using the Tecnomouse, which is a novel hollow fiber bioreactor system. After culture for 30 days in protein-free culture, a total amount of about 14 mg of the recombinant hMAb AE6F4 was obtained, and was shown to be reactive against lung cancer cells in tissues.. |
| 138. | 河本正次、＃井上祐一、篠崎雄一、片倉喜範、立花宏文、白畑實隆、村上浩紀, Impaired tumor phenotypes in class II major histocompatibility complex antigen-inducible cells originated from human lung adenocarcinoma, Biochem. Biophys. Res. Commun., ２１５, ２８０-２８５, 1995.10. |
| 139. | Seiji Kawamoto, Shuichi Hashizume, Yoshinori Katakura, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami, Epitope in cytochrome C from Candida krusei cross-reacts lung cancer-specific human monoclonal antibody HB4C5, In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, 10.1007/BF02634252, 31, 6, 424-426, 1995.01, [URL]. |
| 140. | Seiji Kawamoto, Masahiro Shoji, Yuko Setoguchi, Masatoshi Kato, Shuichi Hashizume, Akira Ichikawa, Kazuhiro Osada, Yoshinori Katakura, Hirofumi Tachibana, Hiroki Murakami, Molecular cloning of the 31 kDa cytosolic phospholipase A2, as an antigen recognized by the lung cancer-specific human monoclonal antibody, AE6F4, Cytotechnology, 10.1007/BF00749397, 17, 2, 103-108, 1995.01, [URL], The human monoclonal antibody AE6F4 specifically reacts with human lung cancer tissues but does not with normal tissues. This monoclonal antibody recognizes a cytosolic 31 kDa antigen in the cancer cells. In a previous study, we elucidated that the 31 kDa antigen belonged to a family of proteins collectively designated as 14-3-3 proteins, which were known as protein kinase-dependent activators of tyrosine/trytophan hydroxylases, or protein kinase C inhibitor proteins. Here we report molecular cloning of the 31 kDa antigen from the human lung adenocarcinoma cell line, A549. Sequencing analysis indicates that the cloned cDNA is identical to that of previously reported human placental cytosolic phospholipase A2 (cPLA2), which is also a member of the 14-3-3 protein family. Western analysis demonstrated that a 31 kDa recombinant cPLA2 expressed in monkey COS cells was recognized by the AE6F4 monoclonal antibody. Binding of the monoclonal antibody to the recombinant cPLA2 was abolished when treated with sodium periodate, suggesting that not only are carbohydrate chains associated with the cPLA2, but they also play a crucial role in antigen recognition by the monoclonal antibody.. |
| 141. | Seiji Kawamoto, Shuichi Hashizume, Yoshinori Katakura, Hirofumi Tachibana, Hiroki Murakami, Molecular cloning of yeast cytochrome C-like polypeptide expressed in human lung carcinoma An antigen recogizable by lung cancer-specific human monoclonal antibody, In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, 10.1007/BF02634095, 31, 9, 724-729, 1995.01, [URL], We previously determined the amino acid sequence to the epitope (ATLFKTR) of cytochrome c from Candida krusei, which is cross-reactive to the lung cancer-specific human monoclonal antibody HB4C5. Here we report that an antigen messenger RNA, which codes for a structure similar to the cytochrome c epitope, is expressed in the human lung adenocarcinoma A549. Sequencing analysis has revealed that this messenger RNA encodes a novel 190 amino acid polypeptide of 21-kDa containing an amino acid sequence (ALLFFT) similar to the cytochrome c epitope, although the total messenger RNA sequence is apparently different from the cytochrome c messenger RNA. Western analysis indicated that an antibody-recognizable 21-kDa antigen which has the same molecular weight as the predicted polypeptide is expressed in the A549 adenocarcinoma. The in vitro translated product of the antigen messenger RNA and synthesized ALLFFT peptide were both shown to be reactive with the monoclonal antibody, indicating that this protein contains the epitope which enables A549 cells to specifically react with the antibody. The antigen mRNA was not expressed in non-transformed fibroblasts, suggesting that the antigen mRNA expression was associated with cellular transformation. Also in part of the antigen nucleotide sequence, there was a segment that had about 90% homology to the long terminal repeat sequence (no. 297–475) of the human endogenous retrovirus HERV-K10, which was related to the mouse mammary tumor virus.. |
| 142. | Yoshinori Katakura, Mamoru Totsuka, Akio Ametani, Shuichi Kaminogawa, Tryptophan-19 of β-lactoglobulin, the only residue completely conserved in the lipocalin superfamily, is not essential for binding retinol, but relevant to stabilizing bound retinol and maintaining its structure, Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 10.1016/0167-4838(94)90051-5, 1207, 1, 58-67, 1994.07, [URL], Residue 19 of tryptophan in bovine β-lactoglobulin (β-LG) is the only invariant residue throughout the lipocalin superfamily having two characteristic features: binding ability for small hydrophobic molecules and the unique β-barrel three-dimensional structure. In this study, we investigated whether this strictly conserved Trp-19 of β-LG would be indispensable for its structure and function such as maintaining the molecular structure and biological activity of β-LG. Spectroscopic and enzymatic oxidation experiments on retinol bound to W19Y, in which Tyr was substituted for Trp-19, showed that Trp-19 was not critical for this binding, but was important for stably maintaining the environment surrounding retinol and the bound retinol. An analysis, using four anti-β-LG monoclonal antibodies as probes, revealed a structural change in region 20-29, but not in the reverse region of Trp-19. A guanidine hydrochloride-induced unfolding study showed that the conformational stability of W19Y was greatly reduced by 6.9 kcal/mol compared to that of wild-type β-LG. These facts indicated that Trp-19 is one of the important residues in correctly maintaining the local structure of β-LG and stably retaining its overall structure, thereby conserving the bound retinol molecule.. |
| 143. | M. Hattori, A. Ametani, Y. Katakura, M. Shimizu, S. Kaminogawa, Erratum Unfolding/refolding studies on bovine β-lactoglobulin with monoclonal antibodies as probes. Does a renatured protein completely refold? (Journal of Biological Chemistry (1993) 268 (22414-22419)), Journal of Biological Chemistry, 269, 14, 1994.01. |
| 144. | M. Hattori, A. Ametani, Y. Katakura, M. Shimizu, S. Kaminogawa, Unfolding/refolding studies on bovine β-lactoglobulin with monoclonal antibodies as probes. Does a renatured protein completely refold?, Journal of Biological Chemistry, 268, 30, 22414-22419, 1993.11, We investigated whether any local moieties within a protein molecule could completely refold from the denatured state to regain the native conformation. Bovine β-lactoglobulin (β-LG) was denatured in the presence of guanidine hydrochloride (GdnHCl) as the denaturant. Renaturation of the denatured β- LG was attempted by dialyzing to remove GdnHCl. The renatured molecules regained the same retinol binding activity as that of native β-LG, and physicochemical studies also indicated that refolding of the denatured β-LG had been almost completely successful. Local structural differences between the native and renatured β-LG molecules were evaluated by using our panel of four anti-β-LG monoclonal antibodies (anti-β-LG mAbs). The structures of the epitope regions in native β-LG recognized by two of these mAbs were the same as those in renatured β-LG. However, it is notable that the binding properties of the other two mAbs to native β-LG indicated a wide structural difference in the epitope regions between the native and renatured β-LG. These regions unable to completely refold were the same as those that unfolded preferentially to the α-helix region, shown in the previous report (Kaminogawa, S., Shimizu, M., Ametani, A., Hattori, M., Ando, O., Hachimura, S., Nakamura, Y., Totsuka, M., and Yamauchi, K. (1989) Biochim. Biophys. Acta 998, 50-56). Complete refolding was never attained by several renaturation conditions such as quicker or slower removal of the denaturant, nor by additional oxidation treatment after reducing the disulfide bonds. These results suggest that some specific moiety(ies) in a protein molecule cannot return to the native conformation from a denatured state, even if the other moieties refold completely, and that such a conformational difference between renatured and native forms has no affect on the biological function of ligand binding.. |
| 145. | Mamoru Totsuka, Yoshinori Katakura, Makoto Shimizu, Shuichi Kanminogawa, Izumi Kumagai, Kin ichiro Miura, Expression and Secretion of Bovine β-Lactoglobulin In Saccharomyces cerevisiae, Agricultural and Biological Chemistry, 10.1271/bbb1961.54.3111, 54, 12, 3111-3116, 1990.01, [URL], A bovine β-Mactoglobulin (β-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-β-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-β-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYG100, a yeast expression vector. In the supernatant of the yeast growth medium, β-LG with a native conformation was detected by sandwich ELISA, and its amount was estimated to be 1.1 mg/1. A Western-immunoblotting analysis revealed that β-LG secrected in the growth medium had the same mobility as that of authentic bovine β-LG. The N-terminal sequence was also identical with that of authentic mature bovine β-LG.. |
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