Kazuhiro Iiyama | Last modified date:2023.11.27 |
Associate Professor /
Agricultural Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture
Department of Bioresource Sciences
Faculty of Agriculture
Graduate School
Undergraduate School
E-Mail *Since the e-mail address is not displayed in Internet Explorer, please use another web browser:Google Chrome, safari.
Homepage
https://kyushu-u.elsevierpure.com/en/persons/kazuhiro-iiyama
Reseacher Profiling Tool Kyushu University Pure
http://www.agr.kyushu-u.ac.jp/lab/plantpp/
A research outline, research achievements, a research theme, etc. .
Phone
092-802-4563
Academic Degree
PhD
Country of degree conferring institution (Overseas)
No
Field of Specialization
Plant pathology, Biological control, Insect pathology, Bacteriology
Total Priod of education and research career in the foreign country
00years02months
Outline Activities
Evolution in virulence, and genetic diversity analyses are carried out by using genome information of plant pathogenic bacteria. Isolation and identification of bacteria that have biological control activity against Ralstonia solanacearum, an plant pathogenic bacteria are conducted. Furthermore, the effects of microorganisms being virulence to pests on plant pathogens are investigated.
Research
Research Interests
- Studies on the evolution in pathogenesis
keyword : pathogenicity, evolution, genome comparison
2017.01. - Studies on the dual biological control against plant pathogen and insect
keyword : plant pahogen, entomopahogenic organism, virulence, pathogenicity
2017.01. - Studies on the pathogenicity of plant pathogenic bacteria
keyword : plant pahogenic bacteria, virulence, pathogenicity
2017.01. - Study on pathogenicity of Hafnia sp. to insects
keyword : Hafnia sp. pathogenicity silkworm
2015.04~2018.03. - Genome analysis of Paenibacillus popilliae and evolution in pathogenicity
keyword : Genome analysis, Paenibacillus popilliae, evolution, pathogenicity
2012.04~2016.04. - Pathogenicity of Pseudomonas aeruginosa in insect hosts
keyword : Pseudomonas aeruginosa, Bombyx mori, virulence
2006.04~2019.02. - Study on phospholipases produced by Pseudomonas aeruginosa
keyword : Pseudomonas aeruginosa, Bombyx mori, phospholipase C, plcB, plcH, plcN
2006.04~2011.01. - Study on superoxide dismutases produced by Pseudomonas aeruginosa
keyword : Pseudomonas aeruginosa, Bombyx mori, virulence,superoxide dismutase,sodM, sodB
2004.04~2012.05Pseudomonas aeruginosa is an opportunistic pathogen of insects and possesses a manganese- and an iron- superoxide dismutases (SODs). To ascertain the role of these SODs in virulence to the silkworm, Bombyx mori, we have generated three mutants carrying an interrupted sodM (encoding manganese- SOD, Mn-SOD) or sodB (encoding iron- SOD, Fe-SOD) and a sodM sodB double mutation. Total SOD activities of sodM, sodB and sodM sodB mutants in Luria broth and tryptic soy broth were ca. 100%, 0% and 0% of the activity of a wild-type strain, respectively. Whereas the relative activities in low phosphate-succinate broth were 0%, 96% and 0%, respectively. This result indicates that the two SODs of P. aeruginosa are highly regulated by the environmental conditions. To investigate their virulence to the silkworm, the fourth instar larvae were inoculated with various doses of the wild-type and three mutants. The virulence remarkably differed among these strains. The sodM mutant was indistinguishable from the wild-type in aspect of virulence at all inoculation levels (103-106 cells / larva). Whereas the virulence of sodB mutant reduced, when relatively low cells number (103-104) was inoculated. Moreover, the double mutant could not cause the any fatal damage at 105 cells or lower. These results suggest that the SODs are required for full virulence of P. aeruginosa to the silkworm and Fe-SOD is more important than Mn-SOD in the infection process.. - Study on the toxic protein produced by the bacteria isolated from insect
keyword : entomopathogenic bacteria, bacterial isolates from ant lion, virulence, virulence factor
2003.04~2015.06. - Study on a two component, GacS-GacA in an oppotunistic bacteria Pseudomonas aeruginosa
keyword : entomopathogenic bacteria, two component system
2001.04~2013.01To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA in C1 (pgacA) was detected. In addition, the complemented mutant restored the level and timing of pyocyanin production, indicating that functional GacA is produced in the complemented strain. However, no significant difference was observed between C1 and C1 (pgacA) with respect to the killing of B. mori larvae..
Papers
Presentations
Educational
Educational Activities
Research about the newest technical journal announcement paper related to plant pathology, plant pathogenic microbiology.
Study about the characterization, infection / multiplication style, a host and a pathogen interaction, plant pathology.
The instruction of an experiment and advice which are performed with the latest technology.
Study about the characterization, infection / multiplication style, a host and a pathogen interaction, plant pathology.
The instruction of an experiment and advice which are performed with the latest technology.
Unauthorized reprint of the contents of this database is prohibited.