Kyushu University [Tetsuo Kondo (Professor) Faculty of Agriculture, Department of Agro-environmental Sciences]

Biomaterials comprising sustainable carbohydrate polymers such as cellulose and chitin are extensively studied under my supervision. Those materials are hierarchically built in the biosynthesizing process from molecular levels to the nano-scale and from the nano to larger scales. Focusing on the building process and the established hierarchical structures is of importance to understand the natural material science and thereby to create a new concept for developing functionalized biomaterials. Therefore, we are attempting to first understand the in situ biological system for fiber- or pellicle-production, and then to modify the process by a physicochemical method in order to obtain a novel ordered 3D structure. For that, studies are conducted in interdisciplinary scientific areas among chemistry, biology, physicochemistry and polymer chemistry, leading to provide a novel functional biomaterial based on the unique 3D structures. In this connection, students under me are now educated and supervised in their research to be able to expand their scientific understanding and create a new research direction.

Research

Research Interests

New form of cellulose nanofibers from wood and bamboo pulp: cellulose nanoanemone
keyword : Cellulose nanofiber, ACC method, Cellulose nanoanemone, bamboo
2016.04～2020.03.
Novel hollow fiber spinning of callose in vitro by using a micro-channel culture system with enzyme complexes from the protoplast cells
keyword : Protoplast, membrane protein, callose, hollow fiber spinning, micro-channel culture system
2014.04～2017.03.
Novel microbial spinning of nanofibers using micro-channel culture of Gluconacetobacter xylinus
keyword : Cellulose, bacterial nanospinning, microchannel, nanofiber, gel, 3D-nanofiber netowork, ordered flow
2011.04～2014.03.
Nematic ordered cellulose templates mediating 3D-ordered biomimic fabrication
keyword : cellulose ,templates ,biomimic fabrication
2010.04～2013.03Nano-cellulose prepared from microbial cellulose pellicle-its surface structure and supermolecular charcteristics-.
Dependence of the Size of Cellulose Fibers at Nano Levels　Prepared by the Counter Collision in Water upon the Enzymatic Degradation
keyword : biomass,the aqueous Counter Collision
2006.10Dependence of the Size of Cellulose Fibers at Nano Levels　Prepared by the Counter Collision in Water upon the Enzymatic Degradation.
Nano-cellulose prepared from microbial cellulose pellicle-its surface structure and supermolecular charcteristics-
keyword : nanocellulose , acetobacter,the aqueous counter collision ,supermolecular charcteristics-
2006.09Nano-cellulose prepared from microbial cellulose pellicle-its surface structure and supermolecular charcteristics-.
Cascade-type utilization of wastes from food industries using the counter collision method in water
keyword : the aqueous counter collision ,Cascade-type utilization
2006.06Cascade-type utilization of wastes from food industries using the counter collision method in water.
Nonocomposite of cellulose/PLA prepared using the counter collision method in water
keyword : cellulose,PLA,Nonocomposite,the aqueous counter collision
2005.04～2012.03Nonocomposite of cellulose/PLA prepared using the counter collision method in water.
Fabrication of ordered hyaluronan sheet epitaxially deposited on the nematic ordered cellulose template
keyword : nematic ordered cellulose ,template, hyaluronan ,epitaxially deposited
2003.04Fabrication of ordered hyaluronan sheet epitaxially deposited on the nematic ordered cellulose template.
Imogolite co-orientated with the cellulose molecular chains on the surface of nematic ordered cellulose template
keyword : nematic ordered cellulose ,template,Imogolite
2003.04Imogolite co-orientated with the cellulose molecular chains on the surface of nematic ordered cellulose template.
Fabrication of 3D honeycomb-patterned compartments for single cell incubation
keyword : 3D honeycomb-patterned ,
2003.04～2012.03Fabrication of 3D honeycomb-patterned compartments for single cell incubation.
Development of functionalized LB membrane from cellulose derivatives.
keyword : cellulose ,LB membrane , functionalized
2003.04～2008.03Development of functionalized LB membrane from cellulose derivatives..
Water-solubilization of crystalline carbohydrate polymers by the counter collision in water
keyword : the aqueous counter collision ,crystalline carbohydrate polymers ,water-solubilization
2002.07Water-solubilization of crystalline carbohydrate polymers by the counter collision in water.
Regulation of deposition pattern of bacteria cellulose ribbons on the physically scratched nano-tracks.
keyword : nano-tracks,bacteria cellulose ,
2002.04Regulation of deposition pattern of bacteria cellulose ribbons on the physically scratched nano-tracks..
Ordered characteristic surfaces with various scales prepared by deposition of callose fibers onto the nematic ordered cellulose substrate
keyword : nematic ordered cellulose ,callose, protoplasts
2002.04Ordered characteristic surfaces with various scales prepared by deposition of callose fibers onto the nematic ordered cellulose substrate.
Secretion of non-crystalline cellulose from M. nivale at a low temperature
keyword : a low temperature,M. nivale ,non-crystalline ,cellulose
2002.04～2015.03Secretion of non-crystalline cellulose from M. nivale at a low temperature.
Characterization of compressed monolayer structure from regioselectively substituted cellulose ethers using various surface analyses
keyword : cellulose,monolayer ,surface analyses
1999.04～2008.03Characterization of compressed monolayer structure from regioselectively substituted cellulose ethers using various surface analyses.
Biodirected epitaxial nanodeposition of bacterial cellulose on oriented macromolecular templates to regulate conctruction of the 3D-structure
keyword : Nematic Ordered Cellulose, epitaxial nanodeposition
1998.04Biodirected epitaxial nanodeposition of bacterial cellulose on oriented macromolecular templates to regulate conctruction of the 3D-structure.
Secretion of a single gigantic callose fiber from a Betula protoplast.
keyword : protoplast,callose ,fiber,stress
1998.04Secretion of a single gigantic callose fiber from a Betula protoplast..

Current and Past Project

Cascade-type utilization of wastes from food industries using the counter collision method in water
The project aimed at the development of energy-minimized system to fabricate biomaterials with ordered structue by biodirected nanodeposition of polymers on oriented macromolecular templates using Acetobacter xylinum.
MAFF Nanotechnology Project at Agriculture, Forestry and Fisheries Research Council. This project aims at developemnt of new materials and nanobiotechnology by regulating various order at nano and micro scales.
Fukuoka Nonotechnology project:
Title:Fabrication of 3D honeycomb-patterned compartments for single cell incubation

Academic Activities

Books

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1.	Tetsuo Kondo, Przemystaw Rytczak, Stanislaw Bielecki, Bacterial NanoCellulose from Biotechnology to Bio-Economy, Elsevier, CHAPTER 4. Bacterial NanoCellulose Characterization pp.59-71, 2016.07.
2.	Noriko Hayashi, Tetsuo Kondo, Enzymatically Produced Nano-ordered Elements Containing Cellulose Iβ Crystalline Domains of Cladophora Cellulose, Springer-Verlag Berlin Heidelberg 2015, 10.1007/978-3-642-45232-1_58, Handbook of Polymer Nanocomposites. Processing, Performance and Application (Volume C: Polymer Nanocomposites of Cellulose Nanoparticles) PP.1-14, 2015.10.
3.	近藤哲男, Bacterial NanoCellulose: A Sophisticated Multifunctional Material, CRC Press , CHAPTER 6. Nematic Orderd Cellulose Templates pp.113-142, 2012.11.

Reports

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1.	Keep on Challenging with Nanocellulose vs Design.
2.	A Unique structure-proper relationship of ACC nanocellulose generated by a "gentle" nano-pulverization process, Aqueous Counter Collision (ACC).
3.	A Unique "Gentle　Nano-Pulverization Process for Native Cellulose Fiber" : Aqueous Counter Collision (ACC).
4.	A "gentle nano-pulverization process" for environmentally friendly cellulose : Aqueous Counter Collision (ACC).
5.	Preparation of cellulose gel from the Licl / DMAc solution.
6.	Preparation of Single Cellulose Nanofibers Dispersed in Water Using Aqueous Counter Collision Method.
7.	A Bacterium Spinning a Cellulose Nanofiber.
8.	Application of the Aqueous Counter Collision Method for Industrial Food Wastes Free Society.

Papers

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1.	Ryo Takahama, Honami Kato, Go Takayama, Kenji Tajima, Tetsuo Kondo, Physical characteristics and cell-adhesive properties of in vivo fabricated bacterial cellulose/hyaluronan nanocomposites, Cellulose, 10.1007/s10570-022-04480-2, 29, 6, 3239-3251, 2022.03.
2.	Shingo Yokota, Airi Nishimoto, Tetsuo Kondo, Alkali-activation of cellulose nanofibrils to facilitate surface chemical modification under aqueous conditions, Journal of Wood Science, 10.1186/s10086-022-02022-9, 68, 1, Article number 14(2022), 2022.03.
3.	Daisuke Tatsumi, Atsushi Kanda, Tetsuo Kondo, Characterization of mercerized cellulose nanofibrils prepared by aqueous counter collision process, Journal of Wood Science, 10.1186/s10086-022-02019-4, 68, 1, Article number 13(2022), 2022.03.
4.	Hikari Utsunomiya, Yutaro Tsujita, Tetsuo Kondo, Cellulose nanoanemone: an asymmetric form of nanocellulose, Cellulose, 10.1007/s10570-021-04231-9, 29, 5, 2899-2916, 2021.10.
5.	Ryo Takahama, Honami Kato, Kenji Tajima, Satomi Tagawa, Tetsuo Kondo, Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter, Biomacromolecules, 10.1021/acs.biomac.1c00987, 22, 11, 4709-4719, 2021.10.
6.	Gento Ishikawa, Tsubasa Tsuji, Satomi Tagawa, Tetsuo Kondo, Adsorption of Janus-type Amphiphilic Cellulose Nanofibrils onto Microspheres of Semi-crystalline Polymers, Macromolecules, 10.1021/acs.macromol.1c01163, 54, 20, 9393-9400, 2021.10.
7.	＃Koichiro Ishida, Shingo Yokota, Tetsuo Kondo, Emulsifying Properties of α-Chitin Nanofibrils Prepared by Aqueous Counter Collision, Journal of Fiber Science and Technology, 10.2115/fiberst.2021-0022, 77, 8, 203-212, 2021.08.
8.	Koichiro Ishida, Shingo Yokota, Tetsuo Kondo, Localized surface acetylation of aqueous counter collision cellulose nanofibrils using a Pickering emulsion as an interfacial reaction platform, Carbohydrate Polymers, 10.1016/j.carbpol.2021.117845, 261, Carbohydrate Polymers 261 (2021) 117845, 2021.06, ナノサイズの繊維状ビルディングブロックの自己組織化は、生体模倣設計および階層的に構築されたバイオベースの材料の構築に不可欠である。そのようなナノフィブリルの表面上の疎水性部分の局在化は、水系における階層的集合の鍵となる。この研究では、両親媒性セルロースナノフィブリル（CNF）を含む独自の自己組織化繊維ビルディングブロックが、水性カウンターコリジョン（ACC）によって調製された。この研究の目的は、ピッカリングエマルションの油/水界面でACC-CNFの表面を選択的にアセチル化することにより、ACC-CNFの表面特性を制御することである。ACC-CNFが反応試薬を含む油滴の表面に吸着されると、局所的な界面反応が発生した。このようなアセチル化反応は、元のCNFの結晶化度と繊維形態を維持しながら達成された。本明細書に記載のアセチル化ACC-CNFからキャストされたフィルムの表面は、均質な分散液で調製されたアセチル化ACC-CNFからキャストされたフィルムの表面とは著しく対照的な独特の自己凝集特性を有していた。.
9.	Gento Ishikawa, Tetsuo Kondo, Characterization of dual nano-size effects of ACC-cellulose nanofibrils on crystallization behavior of hydrophilic poly(vinyl alcohol), Journal of Wood Science, 10.1186/s10086-021-01957-9, 67, 1, Article number 25(2021), 2021.03.
10.	Shingo Yokota, Satomi Tagawa, Tetsuo Kondo, Facile surface modification of amphiphilic cellulose nanofibrils prepared by aqueous counter collision, Carbohydrate Polymers, 10.1016/j.carbpol.2020.117342, 255, Carbohydrate Polymers 255 (2021) 117342, 2021.03, 本研究は、水性カウンターコリジョン（ACC）によって調製されたセルロースナノフィブリル（CNF）の表面の化学修飾に関するものである。ACCによって調製された木材由来のCNFは、水性分散液中で無水酢酸でアセチル化された。中程度にアセチル化されたナノフィブリルは、未修飾のCNFよりも水に容易に分散したが、結晶セルロースIを含む元のナノファイバーの形態はほとんど変化しなかった。これは、結晶性CNFの表面が選択的にアセチル化されており、ナノフィブリル間の自己凝集を阻害し、それによって水性媒体への分散を促進したことを示している。添加剤がないにもかかわらず、アセチル化されたCNFは疎水性表面に容易に吸着され、水との適合性を維持した。これにより、エマルジョンを安定させ、プラスチック樹脂粒子を水中でコーティングする能力が向上した。結果は、ACCによって調製されたCNFの両親媒性は、この簡単な表面アセチル化法によって制御できることを示す。これにより、さまざまな分野での有用性が高まる可能性がある。.
11.	Satomi Tagawa, Koichiro Ishida, Tsubasa Tsuiji, Tetsuo Kondo, Facile size evaluation of cellulose nanofibrils adsorbed on polypropylene substrates using fluorescence microscopy, Cellulose, 10.1007/s10570-021-03759-0, 28, 5, 2917-2929, 2021.03.
12.	Tsubasa Tsuji, Kunio Tsuboi, Shingo Yokota, Satomi Tagawa, Tetsuo Kondo, Characterization of an Amphiphilic Janus-Type Surface in the Cellulose Nanofibril Prepared by Aqueous Counter Collision, Biomacromolecules, 10.1021/acs.biomac.0c01464, 22, 2, 620-628, 2021.01, バイオベースの持続可能な高性能ナノフィブリルとして大きな注目を集めているセルロースナノフィブリルは、主に親水性であると考えられている。この研究は、水性カウンターコリジョン法によって調製されたセルロースナノフィブリル（ACC-CNF）の疎水性および親水性面を備えた水中の両親媒性「ヤヌス型繊維表面」の存在を証明することを目的とした。ACC-CNFの表面特性は、炭水化物結合モジュールと、セルロース繊維表面の疎水性平面のコンゴーレッドプローブ、および親水性平面プローブとしてのカルコフルオルホワイトを使用した共焦点レーザー走査顕微鏡によって明らかにした。結果は、両親媒性ヤヌス型構造を検証する単一のACC-CNF表面上に両方の特徴的な平面が存在することを示した。.
13.	Shintaro Matsuo, Satomi Tagawa, Yudai Matsusaki, Yuri Uchi, Tetsuo Kondo, Callose-synthesizing enzymes as membrane proteins of betula protoplasts secrete bundles of β-1,3-glucan hollow fibrils under ca²⁺-rich and acidic culture conditions, Holzforschung, 10.1515/hf-2019-0142, 74, 8, 725-732, 2020.01, Previously, it was reported that plant protoplasts isolated from Betula platyphylla (white birch) callus secreted bundles of hollow callose fibrils in acidic culture medium containing a high concentration of calcium ions (Ca²⁺). Here, the callose synthase was characterized from in situ and in vitro perspectives. Localization of callose synthases at the secreting site of callose fiber was indicated from in situ immunostaining observation of protoplasts. For in vitro analyses, membrane proteins were extracted from membrane fraction of protoplasts with a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) treatment. The CHAPS extract aggregated in the presence of a high concentration of Ca²⁺, suggesting that Ca²⁺ may promote the arrangement of callose synthases in the plasma membrane. The callose synthase activity was dependent on pH and Ca²⁺, similar to the callose synthase of Arabidopsis thaliana. However, the synthesized fibril products were longer than those produced by callose synthases of herbaceous plants. This is the first insight into the specific properties of callose synthases of woody plants that secrete of callose hollow fibers..
14.	Shingo Yokota, Keita Kamada, Aki Sugiyama, Tetsuo Kondo, Pickering emulsion stabilization by using amphiphilic cellulose nanofibrils prepared by aqueous counter collision, Carbohydrate Polymers, 10.1016/j.carbpol.2019.115293, 226, 2019.12, Cellulose nanofibrils prepared by aqueous counter collision (ACC-nanocelluloses) have specific properties. In this study, the use of ACC-nanocelluloses as emulsifiers and stabilizers was investigated. Oil-in-water Pickering emulsions with long-term stabilities were easily prepared by ultrasonically mixing aqueous ACC-nanocellulose dispersions with non-polar solvents. Stable Pickering emulsions were obtained because the emulsification abilities of the ACC-nanocelluloses were significantly higher than those of cellulose nanofibrils prepared by high-pressure homogenization or other chemical preparation methods. This might be due to exposure of inherently hydrophobic surface planes of cellulose nanofibrils by ACC. The emulsification and stability of the Pickering emulsions were sensitive to the solvent properties such as permittivity, density and viscosity..
15.	Suresh N. Rao, Yutaro Tsujita, Tetsuo Kondo, Surface modification of oriented polysaccharide scaffolds using biotic nanofibers for epidermal cell culture, Cellulose, 10.1007/s10570-019-02615-6, 26, 13-14, 7971-7981, 2019.09.
16.	Yutaro Tsujita, Tetsuo Kondo, A building block of collagen fibrils demonstrated by sequential aqueous counter collision process, Journal of Fiber Science and Technology, 10.2115/fiberst.2019-0014, 75, 9, 112-118, 2019.09.
17.	Satomi Tagawa,Yusuke Yamagishi,Ugai Watanabe,Ryo Funada,Tetsuo Kondo, Dynamics of structural polysaccharides deposition on the plasma-membrane surface of plant protoplasts during cell wall regeneration, Journal of Wood Science, 10.1186/s10086-019-1826-0, 65, 2019.09.
18.	Satomi Tagawa, Tetsuo Kondo, Secretion of a callose hollow fiber from herbaceous plant protoplasts induced by inhibition of cell wall formation, Journal of Wood Science, 10.1007/s10086-018-1726-8, 64, 5, 467-476, 2018.05, セルロースナノファイバーについて、世界を代表する研究者で種々の現象の結果とその応用展開の可能性について論じたものである。.
19.	Gento Ishikawa, Tetsuo Kondo, Thermodynamic effect on interaction between crystalline phases in size-controlled ACC-bacterial nanocellulose and poly(vinyl alcohol), Cellulose, 10.1007/s10570-017-1532-2, 24, 12, 5495-5503, 2017.12.
20.	Aya Nagashima, Tsubasa Tsuji, Tetsuo Kondo, A uniaxially oriented nanofibrous cellulose scaffold from pellicles produced by Gluconacetobacter xylinus in dissolved oxygen culture, Carbohydrate Polymers, 10.1016/j.carbpol.2015.08.077, 135, 1, 215-224, 2016.01.
21.	Ju Fang, Shin Kawano, Kenji Tajima, Tetsuo Kondo, In Vivo Curdlan/Cellulose Bionanocomposite Synthesis by Genetically Modified Gluconacetobacter xylinus, Biomacromolecules, 10.1021/acs.biomac.5b01075, 16, 10, 3154-3160, 2015.10, 最小の遺伝子導入（菌体外分泌機能をつかさどる遺伝子導入なしで）で、合成遺伝子の組み換え効果と同時に物理化学的効果を組み合わせることにより、酢酸菌によりカードランを菌体外に分泌させると同時に、セルロースナノファイバーの生産分泌も同時に行わせることに成功した。このことは、カードラン・ナノセルロース　ナノコンポジットの直接生物製造プロセスの構築を完成させたことになり、それを当該分野の世界的な雑誌「Biomacromolecules」IF=5.750で提案した。.
22.	Yue Zhao, Satoshi Koizumi, Daisuke Yamaguchi, Tetsuo Kondo, Hierarchical structure in microbial cellulose: What happens during the drying process, The European Physical Journal E, 10.1140/epje/i2014-14129-7, 37, Page12(1-12), 2014.12.
23.	Tetsuo Kondo, Ryouta Kose, Hiroki Naito, Wakako Kasai, Aqueous counter collision using paired water jets as a novel means of preparing bio-nanofibers, Carbohydrate Polymers, 10.1016/j.carbpol.2014.05.064, 112, 1, 284-290, 2014.11.
24.	Tetsuo Kondo, Wakako Kasai, Autonomous bottom-up fabrication of three-dimensional nano/microcellulose honeycomb structures, directed by bacterial nanobuilder, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2014.04.002, 118, 4, 482-487, 2014.10.
25.	Tetsuo Kondo, Daisuke Kumon, Akiko Mieno, Yutaro Tsujita, Ryota Kose, Preparaion and characterization of two types of separate collagen nanofibers with different widths using aqueous counter collision as a gentle top-down process, Materials Research Express, 10.1088/2053-1591/1/4/045016, 1, 1-16, 2014.10.
26.	Yohei Kawano, Tetsuo Kondo, Preparation of Aqueous Carbon Material Suspensions by Aqueous Counter Collision, Chemistry Letters, 10.1246/cl.131046, 43, 4, 483-485, 2014.04.
27.	Kunio Tsuboi, Shingo Yokota, Tetsuo Kondo, Difference between bamboo- and wood-derived cellulose nanofibers prepared by the aqueous counter collision method, Nordic Pulp & Paper Research Journal, 10.3183/NPPRJ-2014-29-01-p069-076, 29, 1, 69-76, 2014.02.
28.	Shintaro Matsuo, Akane Takenaga, Tomoko Seyama, Tetsuo Kondo, Secretion of a bundle of(1->3)-β-glucan hollow fibrils from protoplasts of callus suspension under a Ca2+-rich and acidic stressed condition, Holzforschung, 10.1515/hf-2013-0010, 68, 1, 69-73, 2014.01.
29.	Tetsuya Takahashi, Yoko Tsurunaga, Tetsuo Kondo, Fabrication of Highly Isotactic Polypropylene Fibers to Substitute Asbestos in Reinforced Cement Composites and Analysis of the Fiber Formation Mechanism, Journal of Applied Polymer Science, 10.1002/APP.39260, 130, 2, 981-988, 2013.10.
30.	Baoquan Jia, Yutao Li, Bin Yang, Di Xiao, Shengnan Zhang, A. Varada Rajulu, Tetsuo Kondo, Lina Zhang, Jinping Zhou, Effect of microcrystal cellulose and cellulose whisker on biocompatibility of cellulose-based electrospun scaffolds, Cellulose , 10.1007/s10570-013-9952-0, 20, 4, 1911-1923, 2013.08, To investigate the potential application of microcrystal cellulose (MCC) and cellulose whisker (CW) in the electorospun vascular tissue scaffolds,cellulose acetate (CA) and cellulose composite scaffolds containing MCC and CW were electrospun from CA solutions and deacetylation. Structure and morphology of MCC, CW and the fibrous composite scaffolds were inverstigated using FT-IR, SEM, TEM and AFM. The wettability of the scaffolds was evaluated by water contact angle analysis. The effect of MCC and CW on the biocompatibility of the scaffolds for vascular smooth muscle cells (VSMC) was assayed by MTT test, fluorescent imaging and SEM. The biocomposite scaffolds displayed multiscaled structure and morphology. .
31.	Tomoko Seyama, Eun Young Suh, Tetsuo Kondo, Three-dimensional culture of epidermal cells on ordered cellulose scaffolds , Biofabrication , 10.1088/1758-5082/5/2/025010 , 5, 2, 5, 025010, 2013.06.
32.	Lingzhi Zhang, Chengcheng Zhao, Jinping Zhou, Tetsuo Kondo, Fluorescent micelles based on hydrophobically modified cationic cellulose for sensing trace explosives in aqueous solutions, Journal of Materials Chemistry C, 10.1039/c3tc30689e, 1, 36, 5756-5764, 2013.06, Amphiphilic cationic cellulose derivatives with different long alkyl chains as hydrophobic segments were synthesized. They can self-assemble into cationic micelles in distilled water. The structure and properties of the micelles were characterized by elemental analysis, FT-IR, 1H NMR, z-potential measurements, DLS, TEM, and fluorescence spectroscopy. The hydrophobic cores of the micelles were used to load a hydrophobic dye (4,7-bis[4-(1,2,2-triphenylvinyl)phenyl]benzo-2,1,3-thiadiazole, BTPETD) and exhibited a stable photoluminescence. The fluorescence emission can quantitatively and sensitively respond to 2,4-dinitrophenol (DNP) and picric acid (PA) due to the electron transfer between BTPETD and the explosives, and the limit of detection was 200 and 50 nM for DNP and PA, respectively. The novel hydrophobically modified cationic cellulose micelles have the potential to prepare feasible, sensitive and stable sensor systems for detecting explosives in aqueous solutions..
33.	Ana Alonso-Simon, Antonio E. Encina, Tomoko Seyama, Tetsuo Kondo, Penelope Garcia-Angulo, Jesus M. Alvarez, Jose L. Acebes, Takahisa Hayashi, Purification and Characterization of a soluble β-1,4-glucan from bean (Phaseolus vulgaris L.)-cultured cells dehabituated to dichlobenil, Planta, 10.1007/s00425-013-1861-9, 237, 6, 1475-1482, 2013.06.
34.	Ryota Kose, Tetsuo Kondo, Size Effects of Cellulose Nanofibers for Enhancing the Crystallization of Poly (lactic acid), Journal of Applied Polymer Science, 10.1002/app.38308, 128, 2, 1200-1205, 2013.04.
35.	Jun You, Haoze Hu, Jinping Zhou, Lina Zhang, Yaping Zhang, Tetsuo Kondo, Novel Cellulose Polyampholyte−Gold Nanoparticles-Based Colorimetric Competition Assay for the Detection of Cysteine and Mercury(II), Langmuir, 10.1021/la3050913, 29, 16, 5085-5092, 2013.03.
36.	Takahashi, T.,Hoshino, T., Kondo, T., Imura, S., Kudoh, S. and Yoshino, K., Biosynthesis of Microbial Cellulose from the Antarctic Microorganisms, Journal of the Society of Electrical Materials Engineering, 21, 1, 5-10, 2012.12.
37.	Roubroeks, J. P. and Kondo, T., Nano- and microstructures in stretched and non-stretched blend gels of cellulose and hemicelluloses , Holzforschung, 10.1515/hf-2011-0136, 66, 8, 993-1000, 2012.12.
38.	Kondo, T., Kasai, W., Nojiri, M., Hishikawa, Y., Togawa, E., Romanovicz, D. and Brown R Malcolm, Jr., Regulated patterns of bacterial movements based on their secreted cellulose nanofibers interacting interfacially with ordered chitin templates, Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2012.02.0202, 114, 1, 113-120, 2012.07.
39.	Takahashi T, Kondo T, Tanaka K, Hattori S, Irie S, Kudoh S, Imura S, Kanda H ：, Using collagen artificial skin to estimate the protection effects of UV-cut materials against sunlight under the Antarctic ozone hole, Polymer Degradation and Stability , 10.1016/j.polymdegradstab.2013.03.018, 97, 6, 1002-1009, 2012.06, Collagen sheets that simulate human skin were employed to study the protection effects of ultravioletcut (UV-cut) films on the skin when the Antarctic ozone hole appeared. A collagen sheet was covered with a polypropylene film containing zinc oxide and exposed outdoors in the Antarctic. Exposed sheets were dissolved to determine total amino acid amounts. The results show that nearly 2.8 times as much total amino acids were produced in collagen sheets exposed in spring, when the ozone hole appeared, as those produced in collagen sheets exposed in autumn. However, total amino acids in a collagen sheet covered by a film with a zinc oxide content of 0.40 v% decreased to nearly one-fourth the amount in a collagen sheet covered with a zinc-free film, even during spring exposure. Furthermore, analysis shows that total protein and terminal amino group concentration decreased substantially in extracts from collagen sheets with increasing levels of zinc oxide in the film. In other words, the addition of zinc oxide is confirmed to suppress collagen deterioration by UV light very effectively, even if exposure lasts 50 d in spring, when the ozone hole appears. As described above, the collagen sheet method used for evaluation could be used to quantify the protection effects of UV-cut film against high-energy UV light that reaches the ground when the ozone hole appears..
40.	Takahashi T, Kondo T, Tanaka K, Hattori S, Irie S, Kudoh S, Imura S, Kanda H ：, Measurement of solar UV radiation in Antarctica with collagen sheets, Photochemical & Photobiological Sciences, 10.1039/c2pp05365a, 11, 7, 1193-1200, 2012.06, Collagen sheets were used in a unique evaluation method to examine skin damage caused by ultraviolet (UV) light of short wavelength during a season of the Antarctic ozone hole. The collagen sheets were exposed outdoors for 25 and 50 d, in the spring when the ozone hole was formed and in the ozone-holefree autumn. Extracts from the exposed collagen sheets were analyzed for total protein and terminal amino acid concentrations as an index of collagen fragmentation. The results show that the amount of extractable collagen and terminal amino acid concentration in the spring exposure were approximately double and five times higher, respectively, when compared with those in the autumn exposure. During the ozone hole occurrence, the terminal amino acid concentration of the extracted collagen was about five times higher when exposure lasted 50 d from mid-September to the end of October compared to when exposure lasted 25 d from mid-September to early October. This result could be attributed to a limited amount of short-wavelength UV radiation reaching the ground surface as a result of the low height of the sun in September, when the ozone hole occurred. In fact, UV radiation measurements taken at Syowa Station indicate that short-wavelength UV radiation in the range 290–295 nm was not detected until approximately 1–2 months after the beginning of the ozone hole occurrence..
41.	Tetsuya Takahashi, Yoko Tsurunaga, Yuji Aso, Tetsuo Kondo, Sterilization of Spent Bathwater and Washed Fabrics by the Addition of Weakly Acidic Electrolyzed Water, Sen-i Gakkaishi, 68, 6, 149-155, 2012.06.
42.	Tetsuya Takahashi, Yoko Tsurunaga, Yuji Aso, Tetsuo Kondo, The Use of Weakly Acidic Spent Bathwater Mixed with Electrolyzed Water for Laundry, Sen-i Gakkaishi, 68, 6, 156-163, 2012.06.
43.	Seyama T, Kondo T：, Morphological responses of Betula protoplasts in fiber spinning , Holzforschung, 10.1515/HF.2011.158 , 66, 3, 407-411, 2012.03, In a previous study, the nematic ordered cellulose (NOC) templates successfully induced biodirected epitaxial nanodeposition of cellulose nanofibers secreted by Gluconacetobacter xylinus along the orientation of the molecular tracks (Kondo et al. 2002). As an extended concept for the NOC, this article attempts to propose a sort of biomimic mineralization using the template. It combines morphologically controlling process with synthesis of the calcium phosphate as a major component of bones. This process was initially mediated by the modified NOC template having a pair of roles of the ion supply sources and scaffolds for 3D-ordering architecture of the calcium phosphate as a biomineral in the key functions for biomineralization. The successful establishment of such an ordered deposition of the inorganic on the template was confirmed by several surface characterizations such as atomic force microscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and so on. Moreover, similarly to human bones, the obtained major assemble states of the calcium phosphates exhibited amorphous. The above process using the bifunctional cellulose template can be considered as a biomimic mineralization, which also opens pathways toward preparation of potentially versatile organic–inorganic order-patterned composites under a less energy consumption..
44.	Higashi K, Kondo T :, Nematic ordered cellulose templates mediating order-patterned deposition accompanied with synthesis of calcium phosphates, Cellulose , 10.1007/s10570-011-9627-7, 19, 1, 81-90, 2012.02, In a previous study, the nematic ordered cellulose (NOC) templates successfully induced biodirected epitaxial nanodeposition of cellulose nanofibers secreted by Gluconacetobacter xylinus along the orientation of the molecular tracks (Kondo et al. 2002). As an extended concept for the NOC, this article attempts to propose a sort of biomimic mineralization using the template. It combines morphologically controlling process with synthesis of the calcium phosphate as a major component of bones. This process was initially mediated by the modified NOC template having a pair of roles of the ion supply sources and scaffolds for 3D-ordering architecture of the calcium phosphate as a biomineral in the key functions for biomineralization. The successful establishment of such an ordered deposition of the inorganic on the template was confirmed by several surface characterizations such as atomic force microscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, and so on. Moreover, similarly to human bones, the obtained major assemble states of the calcium phosphates exhibited amorphous. The above process using the bifunctional cellulose template can be considered as a biomimic mineralization, which also opens pathways toward preparation of potentially versatile organic–inorganic order-patterned composites under a less energy consumption..
45.	Takahashi T, Aso Y, Kasai W, Kondo T: , Synergetic deodorant effect and antibacterial activity of composite paper containing waste tea leaves , Journal of Wood Science , 10.1007/s10086-010-1171-9, 57, 4, 308-316, 2011.08, Composite paper containing waste tea leaves was prepared to investigate the effective utilization of waste tea leaves as deodorant material. Paper containing waste green tea leaves did not have increased deodorizing ability compared with controls either against acidic odors such as hydrogen sulfide and acetic acid gases or against neutral odors such as formaldehyde and acetaldehyde gases. In contrast, the paper had excellent deodorizing ability against basic odors such as ammonia and trimethylamine gases. It was observed during additional tests conducted on paper samples containing 60 wt% waste leaves of oolong tea, black tea, pu-erh tea, or hojicha, that all the samples reduced the ammonia concentration to below 1 ppm, which is the threshold concentration for olfactory recognition, within 30 min. Further, paper containing waste green tea leaves was found to decrease the odor residual rate to 5.1% in 30 min even for a waste tea leaf content of 10 wt%. The excellent deodorizing ability of the paper could be attributed to the chemical reactions between odorous substances and the catechins found in tea leaves. After the deodorization of ammonia, paper containing waste green tea leaves was found to have increased antibacterial activity against Staphylococcus aureus. .
46.	Kose R, Kasai W, Kondo T :, Switching Surface Properties of Substrates by Coating with a Cellulose Nanofiber Having a High Adsorbability, SEN-I GAKKAISHI, 67, 7, 163-168, 2011.07.
47.	Kose R, Kondo T :, Favorable 3D-network Formation of Chitin Nanofibers Dispersed in Water Prepared Using Aqueous Counter Collision, SEN-I GAKKAISHI, 67, 4, 91-95, 2011.04.
48.	Kose,R., Mitani,I., Kasai, W. and Kondo,T., “Nanocellulose” As a Single Nanofiber Prepared from Pellicle Secreted by Gluconacetobacter xylinus Using Aqueous Counter Collision, Biomacromolecules, 10.1021/bm1013469, 12, 3, 716-720, 2011.03, This study attempted to prepare a single cellulose nanofiber, “nanocellulose”, dispersed in water from 3D networks of nanofibers in microbial cellulose pellicle using aqueous counter collision (ACC), which allows biobased materials to be down-sized into nano-objects only using water jets without chemical modification. The nanocellulose thus prepared exhibited unique morphological properties. In particular, the width of the nanocellulose, which could be controlled as desired on nanoscales, was smaller than that of just secreted cellulose nanofiber, resulting in larger specific surface areas. Moreover, ACC treatment transformed cellulose Iα crystalline phase into cellulose Iβ phase with the crystallinity kept >70%. In this way, ACC method depending on the treatment condition could provide the desired fiber width at the nanoscale and the different ratios of the two crystalline allomorphs between cellulose Iα versus Iβ, which thus opens further pathways into versatile applications as biodegradable single nanofibers..
49.	Takahashi, T.,Aso,Y., Kasai, W. and Kondo,T., Improving the antibacterial activity against Staphylococcus aureus of composite sheets containing wasted tea leaves by roasting , Jounal of Wood Science, 10.1007/s10086-010-1110-9, 56, 5, 403-410, 2010.10.
50.	Iswanto, A.H., Febrianto, F., Wahyudi, I., Hwang, W.J., Lee, S.H., Kwon, J.H., Kwon, S.M., Kim, N.H. and Kondo,T., Effect of Pre-treatment Techniques on Physical, Mechanical and Durability Properties of Oriented Strand Board Made from Sentang wood (Melia excelsa Jack), Journal of the Faculty of Agriculture Kyushu University, 55, 2, 371-377, 2010.10.
51.	Takahashi, T., Kasai, W. and Kondo,T., Effects of Ultraviolet Radiation on the Color of Compounded Papers Containing Wasted Tea Leaves, SEN-I GAKKAISHI, 66, 10, 261-266, 2010.10.
52.	Hishikawa,Y., Togawa,　E. and Kondo,T., Molecular orientation in the Nematic Ordered Cellulose film using polarized FTIR accompanied with a vapor-phase deuteration method, Cellulose, 10.1007/s10570-010-9410-1, 17, 3, 539-545, 2010.06, Previously, the authors reported “Nematic Ordered Cellulose (NOC)” that is a well-ordered state of β-1,4-glucan chains without exhibiting typical X-ray diffraction patterns of any cellulose polymorphs (Togawa and Kondo 1999; Kondo et al. 2001; Kondo 2007). The NOC was prepared by stretching water-swollen gel-like films at the draw ratio of 2.0 to provide highly oriented β-1,4-glucan molecular chains of cellulose, which was proved by the high resolution TEM observation. In this paper, a detailed study of the unique ordered state of the NOC was attempted to characterize orientation of the main chains as well as the OH groups of molecules using polarized FTIR accompanied with a vapor-phase deuteration method. The dichroic analysis suggested that the main chains were fairly oriented in the stretching direction whereas the OH groups remained unoriented. The disordered state of the OH groups regardless of the oriented state for the main chain may hinder the oriented crystallization during the preparation of NOC films..
53.	Takahashi,T., Aso,Y.,Kasai,W. and Kondo,T., Effect of light irradiation on the antibacterial activity of compounded papers containing wasted tea leaves, Jounal of Wood Science, 10.1007/s10086-009-1103-8, 56, 4, 299-306, 2010.04, The endurance of the antibacterial properties of compounded papers containing wasted green tea leaves needs to be examined before considering these papers for long-term use. Hence, compounded papers containing 60 wt% wasted green tea leaves were irradiated for 1–200 h using a xenon lamp to examine the effect of irradiation on antibacterial properties of the paper. Irradiation for 20 h (cumulative ultraviolet dose at 365 nm, 6.67 × 106 J/m2) or longer was found to greatly increase the antibacterial activity of the paper to a level at which no bacterial cell was confi rmed to be viable. The paper was also covered with various glass fi lters and irradiated for 1 h. Irradiation exclusively with visible rays did not signifi cantly affect the antibacterial activity of the paper, whereas irradiation exclusively with ultraviolet rays, even for a short time, greatly increased the antibacterial activity. .
54.	Hesse－Ertelt,S.,Heinze,T.,Togawa,E. and Kondo,T., Structure elucidation of uniformly 13C-labeled bacterial cellulose from different Gluconacetobacter xylinus strains , Cellulose, 10.1007/s10570-009-9355-4, 17, 1, 139-151, 2010.02.
55.	Kondo,T.,Yamamoto,M.,Kasai,W. and Morita,M., Synthesis and properties of regioselectively substituted cellulose cinnamates, ACS SYMPOSIUM SERIES, 1017, 231-241, 2010.02.
56.	Protection Effect for Collagen Artificial Skin of UV-cut Materials in Antarctica.
57.	Akabori,K.,Atarashi,H.,Ozawa,M., Kondo, T.,Nagamura,T. and Tanaka,K, Glass transition behavior of hyper-branched polystyrenes, Polymer, 10.1016/j.polymer.2009.08.029, 50, 20, 4868-4875, 2009.09.
58.	Preparation of Functional Nonwoven Fabric “KAMIKO” Utilizing Wasted Tea Leaves .
59.	Dye Degradation Effect of Rayon Fibers Containing Titanium Oxide Photocatalyst.
60.	Koizumi, S., Tomita, Y., Kondo, T., and Hashimoto, T. , What Factors Determine Hierarchical Structure of Microbial Cellulose -Interplay among Physics, Chemistry and Biology-, Macromolecular Symposia, 279, 110-118, 279,110-118(2009), 2009.05.
61.	Tomita, Y., Kondo, T., Influential Factors to Enhance the Moving Rate of Acetobacter xylinum Due to its Nanofiber Secretion on Oriented Templates, Carbohydrate Polymers, 77, 754-759 (2009), 2009.04.
62.	Fabrication of Microbial Cellulose Nanofaiber Network Sheets Hydrophobically Enhanced by Introduction of a Heat-printed.
63.	Antibacterial Activity of Compounded Paper using Wasted Green Tealeaves Produced by Paper-making Method.
64.	Structure and Mechanical Properties of Papers Containing Ground Wasted Tealeaves.
65.	Hesse－Ertelt,S.,Witter,R.,Ulrich,A.,Kondo,T. and Heinze,T., Spectral assignments and anisotropy data of cellulose Iα:13C-NMR chemical shift data of cellulose Iα determined by INADEQUATE and RAI techniques applied to uniformly 13C-labeled bacterial celluloses of different Gluconacetobacter xylinus strains.,, Magn.Reson.Chem., 46, 1030-1036, 46,1030-1036(2008), 2008.09.
66.	Yuan Mao, Lina Zhang, Jie Cai, Jinping Zhou, Tetsuo Kondo, Effects of Coagulation Conditions on Properties of Multifilament Fibers Based on Dissolution of Cellulose in NaOH/Urea Aqueous Solution, Industrial & Engineering Chemistry Research, 10.1021/ie800833w, 47, 2, 8676-8683, 2008.09.
67.	Kondo, T., Koschella A., Heublein B., Klemm, D., Heinze, T., Hydrogen bond formation in regioselectively functionalized 3-mono-O-methyl cellulose, Carbohydr. Res. , 343,2600-2604(2008), 2008.08.
68.	Koizumi, S., Yue, Z., Tomita, Y., Kondo, T., Iwase, H., Yamaguchi, D.and Hashimoto, T., Bacterium Organizes Hierarchical Amorphous Structure in Microbial Cellulose, European Physics Journal E, 2008.05.
69.	Seyama, T., Kimura, S., Sasamoto, H., Abe, H., Kondo, T., Spinning of a gigantic bundle of hollow fibrils by a spirally moving higher plant protoplast, Planta, 227(6), 1187-1197 (2008), 2008.05.
70.	Kasai,W, Tsutsumi, K, Morita, M and Kondo, T., Orientation of the alkyl side chains and glucopyranose rings in Langmuir-Blodgett films of a regioselectively substituted cellulose ether, Colloid and Polymer Science, 286, 707-712 (2008), 2008.05.

Presentations

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1.	Tetsuo Kondo, A facile fabrication process to impact-absorbing nanocomposites via “Cell Wall Cell Plastics” by surface-coating with amphiphilic Janus-type cellulose nanofibrils, 1st International Symposium on Cellulose and Renewable Materials, 2021.10.
2.	Go Takayama, Shingo Yokota, Tetsuo Kondo, Fabrication of Semi-artificial Cellulose Fiber Production System Using Cellulose Spinning Machinery of Bacteria, 2021 Virtual Annual Meeting of The Korean Society of Wood Science & Technology (KSWST), 2021.04.
3.	Tetsuo Kondo, A Facile Process via "Cell Wall Cell Plastics" to Advance High-Performance in Microplastics by Surface-Coating with Amphiphilic Janus Nanocellulose, 3rd North American iGC Symposium Online 2021, 2021.02.
4.	Masato Kamogawa, Shingo Yokota, Tetsuo Kondo, Crystallization behavior of polypropylene induced by amphiphilic Janus naocellulose as a scaffold, 第69回高分子学会年次大会, 2020.05.
5.	Ryo Takahama, Tetsuo Kondo, Biofabrication of hyaluronic acid / bacterial nanocellulose composited fibers through a biosynthetic-secretory pathway in Gluconacetobacter, EPNOE2019 6th EPNOE International Polysaccharide Conference, 2019.10.
6.	神田敦史, 巽大輔, 近藤哲男, Regenerated cellulose nano-rod obtained by aqueous counter collision, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
7.	大林祐貴, 巽大輔, 近藤哲男, Pickering emulsification characteristics of nanofibers derived from soybean hulls prepared using aqueous counter collision, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
8.	黒柳遥風, 横田慎吾, 近藤哲男, Fabrication of elastomer reinforced with nanocellulose honeycomb cell, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
9.	鴨川正人, 田川聡美, 石川元人, 横田慎吾, 近藤哲男, Crystallization behavior of polypropylene on 3D-honey comb scaffolds of acc-nanocellulose, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
10.	石田紘一朗, 横田慎吾, 近藤哲男, Adsorption properties of ACC-nanocellulose to polystyrene microsphere having different diameter, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
11.	三宅佐和, 横田慎吾, 近藤哲男, Quantification of Adsorption energy of ACC-nanocelluloses at oil/water interface by using pendant drop tensiometry, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
12.	高山剛, 近藤哲男, An attempt to achieve semi-artificial cellulose fiber synthesis using plasma membrane sheets of a bacterium, Acetobacter, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
13.	後藤明希子, 横田慎吾, 近藤哲男, Chemical-free fabrication of bamboo ACC-nanofibril via autoclave pretreatment, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
14.	高濱良, 加藤歩並, 田川聡美, 田島健次, 近藤哲男, Bio-directed assembly of polysaccharides into nanofibers using genetically engineered bacterium, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
15.	辻翼, 坪井国雄, 横田慎吾, 近藤哲男, Surface analysis of amphiphilic janus structure on acc-nanocellulose, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
16.	Wenbo Ye, Tianjuan Jie, Shingo Yokota, Yimin Fan, Tetsuo Kondo, Surface functionalization via TEMPO-mediated oxidation of chitin nanofibers prepared by aqueous counter collision, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
17.	宇都宮ひかり, 近藤哲男, Fluorescent visualization of nanocellulose in water by using modified “cellulose nanoanemone”, 2019 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (19th) and Fibers (17th), 2019.10.
18.	Tetsuo Kondo, Amphiphilic Janus type nanocellulose fabricated by aqueous counter collision: its characteristics and prospect, International Symposium of Renewable and Sustainable Materials 2019 (Materials2019), 2019.08.
19.	Tetsuo Kondo, Process Design Due to Nano-Fusion for Reinforced Nanocomposites by Embedding Nanocellulose Honeycomb Frames, 2019 International Conference of Nanotechnology for Renewable Materials (TAPPI Nano 2019), 2019.06.
20.	Shingo Yokota, Koki Miura, Tetsuo Kondo, Oriented deposition of bacterial nanocellulose induced by nematic ordered cellulose templates with unique surface energy distribution, 257th ACS National Meeting, 2019.04, Ordered deposition of nanocelluloses on the nematic ordered cellulose (NOC) was investigated in the viewpoint of the surface energy distribution of substrates. NOC is a unique template that induces oriented deposition of other objects due to the alternately aligned amphiphilic molecular tracks on its surface. To chenge the surface energy, styrene oligomers were introduced onto the cellulose molecular tracks on the surface of NOC by surface-initiated atom transfer radical polymerization. The introduced hydrophobic moieties were partly converted into hydrophilic by the following UV-irradiation. Surface energy of the resultant templates was quantitatively estimated based on contact angle measurements. Gluconacetobacter xylinus (G. xylinus) cells were then cultivated on the more hydrophilic NOC template above prepared. G. xylinus secreted bacterial cellulose (BC) that was deposited parallel to the oriented molecular tracks on the substrate similar to the original NOC, resulting in a linear movement pattern of the bacteria. However, decrease in the moving rate of G. xylinus and the morphology change of deposited BC indicated less attractive interaction engagements onto the more hydrophilic NOC templates comparing to the original NOC. This suggests that oriented deposition of BC on the NOC is presumably induced by strong hydrophobic interfacial interaction..
21.	Tetsuo Kondo, Gento Ishikawa, Filed surface coating for PET with bamboo-ACC nanocellulose to allow to find a suitable container for the resin-adsorbable nanocellulose, 257th ACS National Meeting, 2019.04, The authors proposed Aqueous counter collision (ACC) process, which is a gentle and rapid method, to prepare separate cellulose nanofibers (ACC-nanocellulose) as dispersion in water using only a pair of water jets, without the need for any chemical modifications. Although the surface of nanocellulose has been widely presumed as highly hydrophilic, the ACC process has shown the capability to impart hydrophobic faces onto the nanocellulose surfaces, resulting in amphiphilic Janus faces. The unique amphiphilic surface properties of non-modified ACC-nanocellulose, exhibited adsorption capability to surfaces on hydrophobic resins such as polypropylene (PP). Therefore, this paper attempts to clarify availability of adsorption for the amphiphilic Janus ACC-nanocellulose onto various surfaces of widely used resins. Not only PP particles but other surfaces were expected to be applicable for the adsorption of ACC-nanocellulose. However, the result exhibited that the adsorption was better for PP rather than other plastic resins, and moreover the ability was found more or less for any plastic resin except PET. In fact, the surface on PET particles indicated almost no adsorption with ACC-nanocellulose. Reversely taking this into consideration, we could find a suitable container for our resin- adsorbable nanocellulose..
22.	Tetsuo Kondo, Shingo Yokota, Eiko Megan Uchida, Gento Ishikawa, Satomi Tagawa, Masato Kamogawa, Design for reinforced nanocomposites having embedded bamboo-ACC nanocellulose honeycomb by fabrication process due to nano-fusion, 257th ACS National Meeting, 2019.04, The authors proposed Aqueous counter collision (ACC) process, which is a gentle and rapid method, to prepare separate cellulose nanofibers (ACC-nanocellulose) as dispersion in water using only a pair of water jets, without the need for any chemical modifications. Although the surface of nanocellulose has been widely presumed as highly hydrophilic, the ACC process has shown the capability to impart hydrophobic faces onto the nanocellulose surfaces, resulting in amphiphilic Janus faces. The ACC-nanocellulose in fact exhibited different surface characteristics from those for other nanofibers prepared in different manners; particularly it appeared in stability of their emulsion, adsorption to hydrophobic resins and others, due to the hydrophobic faces on the ACC-nanocellulose surface. This study involved how such some unique amphiphilic surface properties of the non-modified nanocellulose prepared by ACC can be applied to fabricate nanocomposites directly with polypropylene (PP) without thermal mixing/excluding. The critical process for it is to minimally coat the PP particle as a starting material with ACC-nanocellulose simply by immersing in the diluted aqueous suspension. The coated surface on PP particles are supposed to exhibit the melting point depression due to the interaction between the two components, whereas the cores of the PP are still intact. The DSC curves indicated appearance of the surface melting and then the core melting as heating temperature increased. Therefore, preheating with pressing at the temperature for the surface melting induced fusion among the coating ACC-nanocellulose to form cross-linking, leading to honeycomb frames..
23.	Suresh Rao. N, ＠近藤哲男, A nano-architecture on polysacchride scaffold using ACC biotic nanofibers for skin engineering application, 第33回繊維学会西部支部講演会・見学会, 2018.12.
24.	＠Tetsuo Kondo, Genetically modified bacterial nano-machines for in vivo producing cellulose bio-nanocomposites, International Conference on Pulping, Papermaking and Biotechnology 2018（ICPPB’18）, 2018.11, 基調講演：International Conference on Pulping, Papermaking and Biotechnology 2018（ICPPB’18）、中国・南京市で開催.
25.	Suresh Rao, 近藤哲男, Double phase biopolysacchride scaffolds coated with ACC biotic nanofibers for skin engineering application, セルロース学会第25回年次大会, 2018.07.
26.	Tetsuo Kondo, Preparation of Polypropylene Nanocomposites with Amphiphilic Janus ACC-Nanocellulose Created by Aqueous Counter Collision, 2018 International Conference on Nanotechnology for Renewable Materials（TAPPI）, 2018.06, 2018 TAPPI International Conference on Nanotechnology for Renewable Materials：アメリカ・マディソンで開催.
27.	Takeru Ishihara, Daisuke Tatsumi, Tetsuo Kondo, Characterization of cellulose/cellulose acetate films prepared by coagulation method of blended ionic liquid solution, The 4th International Cellulose Conference（ICC2017）, 2017.10.
28.	Yuri Uchi, Satomi Tagawa, Tetsuo Kondo, Semi-artificial system producing β-1,3-glucan micro-hollow fiber by fixing a single plant cell into micro channel flowing device, The 4th International Cellulose Conference（ICC2017）, 2017.10.
29.	Kunio Tsuboi, Tsubasa Tsuji, Tetsuo Kondo, Mineralization Process In Preparation Of Cellulose Nanofiber-Calcium Carbonate Nanocomposites Produced By The On-site Aqueous Counter Collision Method, The 4th International Cellulose Conference（ICC2017）, 2017.10.
30.	Hikari Utsunomiya, Tetsuo Kondo, “Cellulose nano-anemone” as a Janus nanofiber having nano-tentacles, The 4th International Cellulose Conference（ICC2017）, 2017.10.
31.	Shingo Yokota, Koki Miura, Tetsuo Kondo, Oriented deposition of nanocellulose secreted from Gluconacetobacter xylinus induced by nematic ordered cellulose templates with unique surface energy distribution, The 4th International Cellulose Conference（ICC2017）, 2017.10.
32.	Yukako Hishikawa, Eiji Togawa, Tetsuo Kondo, Characterization of hydrogen bonds in cellulose II crystals using polarized FTIR accompanied with vapor-phase deuteration, The 4th International Cellulose Conference（ICC2017）, 2017.10.
33.	Natsumi Kitazaki, Momoko Kitamikado, Daisuke Tatsumi, Tetsuo Kondo, Viscoelastic Properties of Cellulose Gels Having “Macroscopic Hierarchical Patterns”, The 4th International Cellulose Conference（ICC2017）, 2017.10.
34.	Misa Miyazaki, Daisuke Tatsumi, Tetsuo Kondo, Long periodic structure of mercerized cellulose using X-ray and light scattering measurements, The 4th International Cellulose Conference（ICC2017）, 2017.10.
35.	Kohei Yamashita, Yuka Koga, Daisuke Tatsumi, Tetsuo Kondo, Preparation of optical anisotropic gels from chitin and cellulose, The 4th International Cellulose Conference（ICC2017）, 2017.10.
36.	Gento Ishikawa, Tetsuo Kondo, Dual Nano-size Effects of ACC-nanocellulose Characterized by Poly(vinyl alcohol) Crystallization Behavior as A Probe, The 4th International Cellulose Conference（ICC2017）, 2017.10.
37.	Aki Sugiyama, Tetsuo Kondo, Unique properties of “Green emulsion” using ACC-nanocellulose, The 4th International Cellulose Conference（ICC2017）, 2017.10.
38.	Eiko Megan Uchida, Shingo Yokota, Tetsuo Kondo, Novel nanocomposites prepared from polypropylene micro-particles coated with amphiphilic ACC bamboo nanocellulose, The 4th International Cellulose Conference（ICC2017）, 2017.10.
39.	Suresh Rao, Tetsuo Kondo, Nematically ordered Polysaccharide scaffold coated with ACC treated cellulose nanofibers for skin tissue engineering applications, The 4th International Cellulose Conference（ICC2017）, 2017.10.
40.	Satomi Tagawa, Tetsuo Kondo, Visualization of cellulose deposition onto surfaces of plasma membranes in plant protoplasts during primary cell wall formation, The 4th International Cellulose Conference（ICC2017）, 2017.10.
41.	Ryo Takahama, Tetsuo Kondo, Characterization of Polysaccharide Nanocomposites in vivo Synthesized by Transgenic Gluconacetobacter xylinus, The 3rd Symposium of Bacterial NanoCellulose（BNC2017）, 2017.10.
42.	Suresh Rao, Tetsuo Kondo, Nematically ordered Polysaccharide scaffold coated with ACC treated cellulose nanofibers for skin tissue engineering applications, The 3rd Symposium of Bacterial NanoCellulose（BNC2017）, 2017.10.
43.	Shingo Yokota, Keita Kamada, Mariko Ago, Orlando J Rojas, Tetsuo Kondo, Surface active nanocellulose prepared by the aqueous counter collision method, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
44.	Hikari Utsunomiya, Tetsuo Kondo, “Cellulose nano-anemone” having fibrillated reducing ends as an anisotropic cellulose nanofiber fabricated by the aqueous counter collision, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
45.	Aki Sugiyama, Tetsuo Kondo, Amphiphilic ACC-nanocellulose pickering emulsion as a template for fabrication of three dimensional hierarchical structures, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
46.	Eiko Megan Uchida, Tetsuo Kondo, Novel polypropylene composites with non-modified but amphiphilic bamboo nanocellulose, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
47.	Satomi Tagawa, Yusuke Yamagishi, Ugai Watanabe, Ryo Funada, Tetsuo Kondo, Formation of β-1,3-glucan hollow fiber from plant protoplasts in response to intracellular and extracellular environmental stimuli, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08.
48.	Tetsuo Kondo, Polysaccharide nanotechnology using bio-alchemy and water, EPNOE2017 5th EPNOE International Polysaccharide Conference, 2017.08, 基調講演：5th EPNOE International Polysaccharide Conference （欧州多糖類国際会議2017：ドイツ・イエナで開催）(2017/8/20～8/24) 「Polysaccharide Nanotechnology Using Bio-alchemy and Water」という題目で、水と生物機能を用いる近藤独自のナノテクノロジーについて基調講演を行った。.
49.	Suresh Rao.N, 近藤哲男, Feasibility of nematically ordered polysaccharide templates for skin tissue engineering application, 第54回化学関連支部合同九州大会, 2017.07.
50.	Tetsuo Kondo, Shingo Yokota, Eiko Megan Uchida, Feasible Application of Hydrophobicity in Amphiphilic ACC-Nanocellulose Created by Aqueous Counter Collision (ACC), TAPPI's 2017 International Conference on Nanotechnology for Renewable Materials, 2017.06.
51.	Shingo Yokota, Keita Kamada, Mariko Ago, Orland J Rojas, Tetsuo Kondo, Emulsification Behavior of Amphiphilic Nanocellulose Prepared by Aqueous Counter Collision, TAPPI's 2017 International Conference on Nanotechnology for Renewable Materials, 2017.06.
52.	Tetsuo Kondo, Satomi Tagawa, Visualization of dynamic changing in formation of cell wall cellulose and callose along with arrangements of microtubules with GFP on surfaces of protoplast cells, 253th ACS National Meeting, 2017.04.
53.	Tetsuo Kondo, LB film preparation of regioselectively substituted cellulose cinnamates on nematic ordered cellulose templates , 253th ACS National Meeting, 2017.04.
54.	Tetsuo Kondo, Kunio Tsuboi, Shingo Yokota, Determination of hydrophobicity in amphiphilic nanocellulose imparted by Aqueous Counter Collision (ACC), 253th ACS National Meeting, 2017.04.
55.	Shingo Yokota, Keita Kamada, Tetsuo Kondo, Pickering emulsion stabilized using amphiphilic ACC-nanocellulose, PFI（Paper and Fibre Research Institute）, 2016.10, Stable Pickering emulsion was successfully prepared by using amphiphilic cellulose nanofibers obtained by the aqueous counter collision (= ACC) method using dual water-jets. In the ACC process, water-dispersed single nanofibers (ACC-nanocellulose, (= ACC-Nac)) was prepared through selective cleavage of van der Waals forces in native crystalline cellulose, which would trigger the exposure of the inherent hydrophobic face on the surface of ACC-Nacs. In this study, we investigated regarding ACC-Nacs as an emulsifier. Water dispersions of the ACC-Nac derived from wood-related microcrystalline cellulose were ultrasonically mixed with non-polar solvents. As a result, oil-in-water Pickering emulsions were reproducibly prepared, whereas the O/W emulsion state was negligibly changed at room temperature for a long-time. Besides, the stability of Pickering emulsion from ACC-Nacs was dependent on the permittivity of non-polar solvents. Such an inducing ability of Pickering emulsion with the ACC-Nac was significantly unique and higher than hydrophilic TEMPO-oxidized cellulose nanofibers, which indicates the unique amphiphilic surface properties of ACC-Nacs..
56.	近藤哲男, “Bio-alchemy Using Water and Biological Systems”ーThree-dimensional biofabrication on ordered cellulose templates and ACC-nanodecompositionー, Inha University-seminar, 2016.07.
57.	＠近藤哲男, Pure single cellulose nanofibers of amphiphilic properties with hydrophobic surfaces created by aqueous counter collision, 2016 TAPPI International Conference on Nanotechnology for Renewable Materials, 2016.06.
58.	近藤哲男, Which was the first to appear, β-1,4 or β-1,3 glucans?, 251th ACS National Meeting, 2016.03.
59.	横田慎吾, 西元愛里, 近藤哲男, Surface activation of ACC-nanocellulose for chemical modification in an aqueous system, 251th ACS National Meeting, 2016.03.
60.	菱川裕香子, 近藤哲男, Analyses on hydrogen bonding in noncrystalline regions of regioselectively methylated cellulose films by a combination of vapor-phase deuteration and generalized two-dimensional correlation IR spectroscopy, 251th ACS National Meeting, 2016.03.
61.	横田慎吾, 近藤哲男, Chemical modification of cellulose nanofibers via surface activation in an aqueous dispersion system, EMN Meeting on Cellulose (Energy Materials Nanotechnology), 2016.03.
62.	近藤哲男, Brief introduction on Nanocellulose researches in Japan, 日本・カナダナノセルロース国際シンポジウム, 2016.01.
63.	鎌田啓大, 横田慎吾, 近藤哲男, Pickering emulsion stabilized using amphiphilic cellulose nanofibers prepared by the aqueous counter collision method, The 2015 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2015), 2015.12, This study deals with the amphiphilic surface characteristics of the cellulose nanofibers prepared by the aqueous counter collision (=ACC) method. The ACC method allows bio-based materials to pulverize into nano-objects using dual high-speed water jets. Intermolecular interactions of bio-based materials are cleaved selectively depending on the ejecting pressure. By the ACC-treatment of native cellulose fibers, water-dispersed single nanofibers are obtained, which is termed as “ACC-nanocellulose”. In the crystallization step of cellulose, glucan chains associate to form glucan sheets via hydrogen bonds to establish a situation that glucan sheets are stacked through van der Waals forces between hydrophobic planes. Kinetic energy of ACC is theoretically capable of selective cleavage of van der Waals forces in cellulose fibers, resulting in the exposure of the hydrophobic surface of ACC-nanocellulose. In this study, we investigated the emulsifying behaviors of the aqueous dispersion of thus prepared ACC-nanocellulose with non-polar solvents in order to demonstrate the apparent amphiphilicity. When aqueous dispersions of ACC-nanocellulose derived from wood were ultrasonically mixed with non-polar solvent, Pickering emulsion was formed at the interface between the aqueous phase containing ACC-nanocellulose and non-polar solvent phase. In particular, a stable emulsion was formed using cyclohexane in comparison to n-hexane, toluene, and ethyl acetate. Such Pickering emulsion forming ability of ACC-nanocellulose was significantly unique and higher than hydrophilic TEMPO-oxidized cellulose nanofibers, which strongly indicates the amphiphilic surface properties of ACC-nanocellulose. .
64.	田川聡美, 山岸祐介, 渡邊宇外, 船田　良, 近藤哲男, Production of a β-1,3-glucan hollow fiber due to an environmental stress response in plant protoplasts , The 2015 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2015), 2015.12, In our previous report, it was found that under a Ca2+-rich and acidic condition, protoplasts isolated from white birch (Betula platyphylla) leaves calluses produced a bundle of hollow fibrils consisted of β-1,3-glucan (a callose hollow fiber) due to an environmental stress response. In this unique phenomenon, cortical microtubules (CMTs) were predicted to contribute to formation of the above structure, because the distributions of callose synthases in plasma membrane may be controlled by CMTs as previously reported. Therefore, we investigated changes of CMTs of protoplasts in producing a callose hollow fiber. To visualize CMTs, the mammalian microtubule-associated protein 4 with the green fluorescent protein (GFP-MAP4) gene was introduced into the calluses. When protoplasts prepared from the genetically-modified calluses were cultured under the above stress condition, GFP-MAP4-labeled CMTs surrounded the producing site of a callose hollow fiber in a random manner. To monitor the formation process of a callose hollow fiber, polymerization or de-polymerization of CMTs was inhibited by using oryzalin or paclitaxel, respectively. In the case of paclitaxel, the fiber width became larger when compared with those in a stress condition without inhibitors. In contrast for the case of oryzalin, the fiber width became thinner. These results indicate that changing of CMTs possibly affects the production quantity or self-assembly formation of callose hollow fibrils. This study would provide an understanding why the three-dimensional hollow structure of a biological polysaccharide was formed. .
65.	辻田裕太郎, 近藤哲男, Nano-pulverization of collagen fibrils by aqueous counter collision with assistance of activated water, 2015 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (17th) and Fibers (15th), 2015.11.
66.	宇都宮ひかり, 近藤哲男, "Cellulose nano-anemone” prepared by aqueous counter collision of bacterial nanocellullose, 2nd International Symposium on Bacterial Nanocellulose GDANSK 2015, 2015.09.
67.	近藤哲男, Bacterial cellulose architecture from "molecules" via nano to "3D-materials", 2nd International Symposium on Bacterial Nanocellulose GDANSK 2015, 2015.09.
68.	Ju Fang, 河野信, 田島健次, 近藤哲男, Celllulose/Curdlan Nanocomposites from Gene-engineered Gluconacetobacter xylinus , セルロース学会第22回年次大会, 2015.07.
69.	近藤哲男, Fabrication of "Cellulose Nano-Anemone", 2015 TAPPI International Conference on Nanotechnology for Renewable nanomaterials, 2015.06.
70.	Yukako Hishikawa, Tetsuo Kondo, Characterization of noncrystalline regions in regioselectively methylated cellulosic films using vapor-phase deuteration and generalized 2D correlation infrared spectroscopy, 249th ACS National Meeting, 2015.03.
71.	Tetsuo Kondo, Fabrication and Characterization of cellulose nanoanemone, 249th ACS National Meeting, 2015.03.
72.	Shingo Yokota, Tetsuo Kondo, Surface Reactivity in an Aqueous System of Bio-Nanofibers Prepared by the Aqueous Counter Collision Method , International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
73.	Yohei Kawano, Tetsuo Kondo, Preparation of Aqueous Dispersions of Multi-Walled Carbon Nanotubes by the Aqueous Counter Collision Method , International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
74.	Hikari Utsunomiya, Shingo Yokota, Tetsuo Kondo, Preferential Subfibrillation from Reducing Ends in the Initial Stage of Nano-Pulverization Using Aqueous Counter Collision , International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
75.	Yutaro Tsujita, Shingo Yokota, Tetsuo Kondo, Self-Assembling Behaviors of Collagen Nanofibers in the Aqueous Dispersions Prepared by the Aqueous Counter Collision Method , International Symposium on Fiber Science and Technology 2014 (ISF2014), 2014.09.
76.	Chemical Reactivity on Surface of Cellulose Nanofibers Prepared by the Aqueous Counter Collision Method .
77.	方駒, 近藤哲男, 田島健次, 河野信, 形質転換された酢酸菌より分泌されるカードラン／セルロース複合体, セルロース学会第21回年次大会, 2014.07, Gluconacetobacter xylinus is well known for its remarkable ability to secrect cellulose nanofiber extracellularly. By modifying this natural nanofiber producing system, it’s possible to obtain other kinds of polysaccharide materials or composites. In this study, a novel curdlan/cellulose composite was successfully obtained by transforming a curdlan synthase gene from Agrobacterium sp. to Gluconacetobacter xylinus. The results suggested that the curdlan might be intracellularly synthesized then secreted accompanied with the formed cellulose nanofibers, resulted in the formation of a curdlan-covered pellicle. This study explored a potential approach to obtain polysaccharide composites through biosynthesis..
78.	Different Nano-pulverizing Behaviors in the Aqueous Counter Collision Process of Microbial Cellulose Fibers Secreted by Gluconacetobacter xylinus under Different Culture Conditions .
79.	Change of cell polarity in secreting β-1,3-glucan hollow fibrils of callus protoplasts under a stress culture.
80.	Nanocomposite of Cellulose Nanofiber and CaCO3 Prepared by the On-site Aqueous Counter Collision Method.
81.	Preparation of Thinner Cellulose Nanofibers Using the Modified Aqueous Counter Collision System with a Novel Additive.
82.	Yukako Hishikawa, Tetsuo Kondo, Characterization of noncrystalline regions of cellulose derivatives using vapor-phase deuteration and generalized 2D correlation infrared spectroscopy, 247th ACS National Meeting, 2014.03.
83.	Tetsuo Kondo, Gento Ishikawa, Nanosize dependence of fiber width on interfacial interactions in cellulose nanocomposites with poly(vinyl alcohol), 247th ACS National Meeting, 2014.03.
84.	Yutaro Tsujita, Shingo Yokota, Tetsuo Kondo, Collagen nanofibers as a novel building block prepared by the aqueous counter collision method, 247th ACS National Meeting, 2014.03.
85.	Yohei Kawano, Tetsuo Kondo, Induced water dispersibility of the multi-walled carbon nanotubes by the aqueous counter collision method, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
86.	Kunio Tsuboi, Shingo Yokota, Tetsuo Kondo, Difference between bamboo- and wood-derived cellulose nanofibers prepared by the aqueous counter collision method, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
87.	Liwei Yu, Daisuke TATSUMI, Tetsuo Kondo, Preparation of nano carbon particles from activated carbon using the aqueous counter collision treatment, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
88.	Kazuya Fujiwara, Daisuke TATSUMI, Tetsuo Kondo, Single relaxation behavior in dynamic viscoelastic properties of green seaweed polysaccharide ulvan/AlCl3 aqueous systems, 2013 Kyushu-Seibu/Pusan-Gyeonnam Joint Symposium on High Polymers(16th) and Fibers(14th), 2013.11.
89.	Shingo Yokota, Shiro Sakoda, Tetsuo Kondo, Interfacial Design of Nano-sized and Nano-structured Cellulose Materials by Chemical Modification, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
90.	Ju Fang, Satoshi Nakagawa, Shin Kawano, Kenji Tajima, Tetsuo Kondo, Secretion of cellulose / curdlan nanocomposites by gene-transformed Gluconacetobacter xylinus, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
91.	Saki Nagamoto, Tetsuya Takahashi, Shingo Yokota, Tetsuo Kondo, Polysaccharide nanofibers secreted by the pink snow mold fungus in Antarctica depending on temperature stress, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
92.	Hikari Utsunomiya, Shingo Yokota, Tetsuo Kondo, Preferential cleavage of reducing ends in cellulose fibers for nano-pulverization using aqueous counter collision, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
93.	Takahiro　Kojima, Shingo Yokota, Tetsuo Kondo, Ordered biomineralization mediated by a host-guest reaction on unique oriented polysaccharide templates, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
94.	Tetsuo Kondo, Tomoko Seyama, Three-dimensional biofabrication on nematic ordered cellulose templates, EPNOE2013 3rd EPNOE International Polysaccharide Conference, 2013.10.
95.	方駒, 中川理, 近藤哲男, 田島健次, 河野信, アグロバクテリウム由来のカードラン合成遺伝子の酢酸菌への導入の試み , セルロース学会第20回年次大会, 2013.07, Gluconacetobacter xylinus, a representative cellulose production bacterium, is well-known for its 3-D cellulose fiber secreting ability. In this work, an attempt of introduction of the (1→3)-β-glucan (curdlan) synthesizing ability to Gluconacetobacter xylinus has been carried out by transforming the curdlan synthesizing gene from Agrobacterium sp. ATCC 31749 to Gluconacetobacter xylinus ATCC 23769. The successful construction of the expression vector was confirmed by electroporation and sequence detection. The results showed that the modified G. xylinus lost an ability to form pellicle but secreted polysaccharides-like compounds dispersed in the medium. The composition and structure of the secretion is now under investigation. .
96.	方駒, 中川理, 河野信, 田島健次, 近藤哲男, 遺伝子組換えした酢酸菌によるカードランナノコンポジットファイバーの生産, 第50回化学関連支部合同九州大会, 2013.07, Gluconacetobacter xylinus(G. xylinus) is widely studied because of its excellent 3-D cellulose fiber properties. In this work, we tried to introduce a(1→3)-β-glucan(curdlan) synthesizing ability to G. xylinus by transforming the curdlan synthesizing gene (crdS) from Agrobacterium sp. in order to allow G. xylinus to have a multiple fiber forming capacity to produce a novel curdlan-containing composite nanofiber..
97.	Tetsuo Kondo, Gento Ishikawa, Nano-size effct of cellulose fibers in interfacial interactions for nano-composites with poly(vinyl alcohol), The 17th International Symposium on Wood,Fiber and Pulping Chemistry, 2013.06.
98.	Shingo Yokota, Tetsuo Kondo, Surface acetylation of cellulose-based nanofibers prepared by aqueous counter collision, The 17th International Symposium on Wood,Fiber and Pulping Chemistry, 2013.06.
99.	Tetsuo Kondo, Fabrication of a uniaxially oriented nano-fibrous film by drawing of microbial cellulose pellicle secreted by Gluconacetobacter xylinus under an oxygen–lacking environment, 245th ACS National Meeting and Exposition, 2013.04, A drawable microbial cellulose pellicle having a minimum physical cross-linkage of the nanofibers was secreted by Gluconacetobacter xylinus cultured in a closed space of Schramm-Hestrin culture medium covered with silicone oil for preventing immediate use of the ambient oxygen gas. The crystalline structure of the fibers thus obtained was more than 90 % rich in cellulose I crystalline phase, which the normal culture had not provided to date. Moreover, the obtained pellicle allowed stretching at 1.5 times to provide a novel film with oriented crystalline nanofibers. The mechanical properties and thermal stability exhibited superior to widely used polymeric materials. It was also noted that the heating process induced transformation of the dominant cellulose I crystalline phase into I phase without a loss of the crystallinity and the high Young’s modulus. The microbial culture under an oxygen-lacking stress could offer fabrication of a novel oriented nano-fibrous film of cellulose I promising excellent potential properties. .
100.	Interfacial design of cellulose nano-objects by chemical modification.
101.	Surface acetylation of cellulose nanofibers prepared by aqueous counter collision.
102.	Novel ordered cellulose templates mediating host-guest biomineralization.
103.	Preparation of three-dimensional architecture by living radical polymerization initiated from nematic ordered cellulose .
104.	Preparation of the water-dispersible carbon nanotube using aqueous counter collision.
105.	Gelation behavior of Konjac-glucomannan with different dispersibility.
106.	Preparation of oriented films using a novel cellulose nanofiber gel secreted by Gluconacetobacter xylinus.
107.	Surface modification of cellulose nanofibers prepared by aqueous counter collision.
108.	Attempt for preparation of conductive cellulose seats using fine graphite particles.
109.	An attempt to introduce a fiber-secreting ability of (1→3)-β-glucan to Gluconacetobacter xylinus.
110.	Nanofibres prepared from bio-based materials using aqueous counter collision.
111.	“Biomimic-mineralization" mediated by a bifunctional cellulose template.
112.	Surface modification in an aqueous system of biobased nanofibers prepared by counter collision.
113.	Characterization of single cellulose nanofibers prepared by the aqueous counter collision of pellicles secreted by Acetobacter xylinum.
114.	Size Dependence of Cellulose Nanofibers on The Interfacial Interaction with Poly(Vinyl Alcohol) Molecules in The Composites.
115.	Aqueous counter collision as a novel tool to hierarchically and　rapidly decompose a cellulose fiber into the single nanofibers.
116.	Morphology of single "nanocellulose" prepared from the pellicle of Acetobacter xylinum using aqueous counter collision .
117.	Influential factors to enhance the moving rate of Acetobacter xylinum due to its nanofiber secretion on oriented templates .
118.	Mineralization of hydroxyapatite on nematic ordered cellulose templates.
119.	Investigation of molecular orientation in nematic ordered cellulose films with vapor-phase deuteration and polarized FTIR .
120.	Patterning in movements and deposition of secreted cellulose nanofiber of Acetobacter xylinum directed by an interfacial interaction on ordered chitin templates.
121.	Regulated movements and cellulose nanofiber deposition of Acetobacter Xylinum on polysaccharide templates as a scaffold .
122.	The morphology of cellulose nanofibers obtained using the aqueous counter collisionates.
123.	Synthesis,properties and their LB film preparation of regioselectively substituted cellulose cinnamates .
124.	Synthesis,properties and their LB film preparation of regioselectively substituted cellulose cinnamates .

Membership in Academic Society

International Academy of Wood Science
The Chemical Society of Japan
American Chemical Society
The Polymer Society of Japan
The Cellulose Society of Japan
The Society of Biotechnology, Japan
The Society of Fiber Science and Technology Japan
The Wood Science Society of Japan
The Spectroscopical Society of Japan
The Japanese Society of Applied Glycoscience

Awards

International Academy of Wood Science has elected Prof.Dr.Tetsuo Kondo as Fellow in the year 2010
"Characterization of hydrogen bonds involved in cellulosics using regioselectively substituted cellulose derivatives as cellulose models."

Educational

Educational Activities

Classes:Analytical Chemistry, Biomacromolecular Materials, Biomaterial Science Experiments, Polymer Materials

Other Educational Activities

2021.11.
2019.08.
2018.08.
2017.08.
2016.08.
2015.08.
2014.08.
2013.08.
2012.08.
2011.08.
2010.08.

Social

Professional and Outreach Activities

Working as an evaluater for NSF projects and others in USA.　Activities supporting green sustainable science in various places including Wuhan, China..

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