Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Hiroshi Takamatsu Last modified date:2020.10.02

Professor / Bioengineering / Department of Mechanical Engineering / Faculty of Engineering


Papers
1. Tatsuya Yoshikawa, Hiroshi Miura, Tatsuya Hirota, Shunsuke Asai, Masanori Yamashita, Kei Yamada, Mohammed Shurrab, Takanobu Fukunaga, Hiroshi Takamatsu, Fundamental Evaluation of Thermophysical Properties of Lipiodol Associated with Cryoablation
Freezing Experiments Using Lipiodol Phantom, CardioVascular and Interventional Radiology, 10.1007/s00270-019-02401-8, 43, 3, 514-519, 2020.03, Purpose: To elucidate the basic thermophysical properties at low temperatures of lipiodol, which is used as a marker by transarterial injection before CT-guided cryoablation for solid tumors, by fundamental experiments with pure lipiodol phantom. Materials and Methods: The freezing point of lipiodol was measured using differential scanning calorimeter (DSC) by detecting differences in the heating rate during heating from − 30 °C. Freezing experiments were conducted using pure lipiodol and a tissue phantom, which were prepared in an acrylic container at 37 °C. The growth of the frozen region was observed for 10 min. Temperatures were monitored at the cryoprobe surface and designated positions around the cryoprobe. Results: The DSC experiment showed that freezing was observed between − 5 and − 30 °C, which indicated that the freezing point was approximately − 5 °C. Freezing experiments revealed that the diameter of frozen region in the lipiodol was smaller than that in the tissue phantom (5 mm vs 24 mm) after 10-min freezing. The temperature at the probe surface was − 130 °C in lipiodol, which was 25 °C lower than that in the tissue phantom. There was a larger temperature gradient near the cryoprobe in lipiodol due to lower thermal conductivity. Conclusions: The present results suggest that an extremely high concentration of lipiodol (close to pure lipiodol) potentially reduces frozen region because of its lower freezing point and smaller thermal conductivity. However, since lipiodol concentrations in clinical cases differ from the current model, further studies using models that are close to clinical conditions are required. Level of Evidence: No level of evidence, laboratory investigation..
2. Kosaku Kurata, Keita Sumida, Hiroshi Takamatsu, Open-source cell extension system assembled from laser-cut plates, HardwareX, 10.1016/j.ohx.2019.e00065, 5, 2019.04, Biological response of cells to mechanical stimulation plays an important role in regulation of cell activity, and thus has been an attracting topic for researchers in the field of biomechanics for many years. Stretching is the most common type of the mechanical stimulus that has been used for in vitro experiments. The device for application of stretching to cells has commercially been available for more than three decades. Although these ready-made devices will bring a smooth start of research work, they cost several thousands to tens of thousands dollars for introduction. Therefore, this study proposes an open-source self-made cell extension system which can be built at one tenth to one hundredth of the price of commercial devices. To reduce the difficulty of machining, the device was designed to be assembled from acrylic plates fabricated only by using a laser cutter without machining such as drilling or screw thread cutting. All other mechanical elements and electrical components are purchased from web shops. The accuracy of the reciprocating motion was verified with the fabricated device and an elastic silicone container for cell culture..
3. Kosaku Kurata, Shuto Yoshimatsu, Hiroshi Takamatsu, Low-Voltage Irreversible Electroporation Using a Comb-Shaped Contact Electrode, IEEE Transactions on Biomedical Engineering, 10.1109/TBME.2019.2914689, 2019.02, Objective: Irreversible electroporation (IRE) is a less invasive therapy to ablate tumor cells by delivering short intensive electric pulses more than a few kV via needle-like electrodes. For reducing the required voltage for the IRE, a durable comb-shaped miniature electrode was designed to use in contact with the lesion surface for a new method named contact IRE. Methods: A miniature electrode was newly fabricated by a fine inkjet patterning and the subsequent etching of a copper-clad polyimide film. A train of 10-$\mu$s or 100-$\mu$s long electric pulses were applied 90 times at the interval of 1 s to a tissue phantom, and its cross section was observed to measure the necrotized area. Results: Cell experiments showed that the maximum ablation depth increased as a function of the applied voltage and reached 400 $\mu$m at 20 V. Furthermore, insulation of the lateral space between electrode teeth with a resin and administration of adjuvants to reduce the IRE threshold of the cell membrane did increase the ablation depth by 26 % and the ablation area by 40 %. Conclusion: The miniature electrode developed in this study successfully necrotized cells in a tissue phantom 400 $\mu$m deep from the surface with the electric pulses of only 20 V. Significance: The contact IRE for the surface of skin and gastrointestinal tract will ablate cutaneous and subcutaneous tumors by applying only several tens of volts..
4. T. Fukunaga, Y. Okuno, K. Kitamura, H. D. Wang, Kosaku Kurata, Hiroshi Takamatsu, A device for measuring thermal conductivity with a drop of liquid, 16th International Heat Transfer Conference, IHTC 2018 International Heat Transfer Conference, 2018-August, 8955-8960, 2018.08, Measurement of thermal conductivity of liquid was demonstrated with a sample considerably smaller than previously reported methods. The sensor that was named micro-beam sensor is a free-standing platinum strip suspended across a trench on a silicon substrate and heated in a sample liquid by DC. The temperature of the sensor is measured from the electrical resistance of the sensor. The measurement is based on the principle that the temperature rise of the sensor depends on the thermal conductivity of the liquid. The advantage of this method includes: 1) the system has a steady state because the substrate at both ends of the sensor remains at a constant temperature irrespective of heating, 2) the steady state is reached within several hundred microseconds because the sensor is only ~10 μm long, 3) the temperature of the sensor does not change after the initial rise due to heating because the effect of free convection is negligibly small, and 4) the sensor is useful for measuring small samples. Comparing the measured temperature rise with the result obtained by numerical analysis of steady-state heat conduction, we can determine the thermal conductivity of the sample liquid. To demonstrate the advantage of this method, we developed a device that consists of a micro-beam MEMS sensor placed in a micro chamber fabricated in a PDMS block. The fabricated platinum sensor was ~10 μm long, ~0.7 μm wide and 40 nm in thickness. The electrical and thermal properties were first determined as a function of temperature by a calibration experiment in a vacuum chamber together with two-dimensional electrical/thermal analyses. Then the thermal conductivities of four kinds of liquid, i.e. FC-72, toluene, ethanol and methanol, were measured at atmospheric pressure and temperature by injecting a sample of ~30 μL into the chamber with a micropipette. The measured thermal conductivity agreed with literature values within 4 % error..
5. Haidong Wang, Xing Zhang, Hiroshi Takamatsu, Koji Takahashi, Effective thermal rectification in suspended monolayer graphene, 16th International Heat Transfer Conference, IHTC 2018 International Heat Transfer Conference, 2018-August, 6903-6908, 2018.08, Thermal rectification is a phenomenon that the heat flow changes by reversing the direction of temperature gradient. This is a fundamental behavior of the thermal rectifiers, which can be used for the active heat flow control, thermally driven computer, efficient energy harvesting, etc. The key challenge is how to increase the thermal rectification ratio, which is defined as the relative change of thermal conductivities in different heat flow directions. Due to the significant size effect and unique heat transfer mechanisms, nanomaterials (such as carbon nanotubes, nanowires, graphene, etc.) are suggested to have high thermal rectification ratio. However, the experiment result showed that the ratio of the single carbon nanotube thermal rectifier was only 7%. In the past decade, many theoretical researches and molecular dynamics simulations have shown that the monolayer graphene may have high thermal rectification ratio due to its unique two-dimensional heat transfer mechanism. But the experimental work is still a blank because of the difficult fabrication process of suspended graphene electronic device. In this work, we report the experimental demonstration of a suspended monolayer graphene thermal rectifier. Three different types of graphene thermal rectifiers have been fabricated with different asymmetric nanostructures. The focused ion beam manufacturing, electron beam induced deposition and precise electron beam lithography were used to design and create asymmetric nanostructures on the monolayer graphene. The thermal rectification ratios were measured by using a precise H-type sensor method. The highest rectification ratio reaches 28% for the graphene with asymmetric nanopores. The asymmetric dependence of thermal conductivity on temperature and space is known to be the physical reason. For the other two kinds of thermal rectifiers, the rectification ratios are about 10%. The asymmetric phonon scattering is known to be the physical reason, which has been proved by using large-scale molecular dynamics simulation..
6. Hiroshi Takamatsu, Haidong Wang, Takanobu Fukunaga, Kosaku Kurata, Measurement of fluid thermal conductivity using a micro-beam MEMS sensor, International Journal of Heat and Mass Transfer, 10.1016/j.ijheatmasstransfer.2017.09.117, 117, 30-35, 2018.01, A new method for measuring thermal conductivities of gases and liquids was established by demonstrating the measurement of five kinds of liquid and air. It uses a sensor named “micro-beam sensor” that is a ∼10-μm-long free-standing platinum membrane suspended across a trench on a silicon substrate and heated in a sample by DC. This method is unique in that it is a steady-state measurement but free from the effect of natural convection owing to the micrometer size of the sensor. Improving the method for precisely determining the temperature of the sensor and modifying the device from those used in our previous feasibility study, we successfully measured the thermal conductivity ranging from ∼0.03 to ∼0.6 W/(m⋅K) within 4% error..
7. Kosaku Kurata, Junpei Matsushita, Atsushi Furuno, Junichi Fujino, Hiroshi Takamatsu, Assessment of thermal damage in total knee arthroplasty using an osteocyte injury model, Journal of Orthopaedic Research, 10.1002/jor.23600, 35, 12, 2799-2807, 2017.12, Polymethylmethacrylate bone cement has been widely used for the anchorage of artificial implants in various orthopedic surgeries. Although it is one of the most successful biomaterials in use, excess heat generation intrinsically causes thermal damage to bone cells adjacent to the bone cement. To estimate a risk of thermal injury, a response of bone cells to cement polymerization must be elucidated because of the occurrence of thermal damage. Thermal damage is affected not only by maximal temperature but also by exposure time, temperature history, and cell type. This study aimed at quantifying the thermal tolerance of bone cells for the development of a thermal injury model, and applying this model for the estimation of thermal damage during cement polymerization in total knee arthroplasty. Osteocytes, osteoblasts, and fibroblasts were respectively subjected to steady supraphysiological temperatures ranging from 45 to 50°C. Survival curves of each cell and temperatures were used to formulate the Arrhenius model. A three-dimensional heat conduction analysis for total knee arthroplasty was conducted using the finite element model based on serial CT images of human knee. A maximal temperature rise of 50°C was observed at the interface between the 3-mm thick cement and the tissue immediately beneath the tibial tray of the prosthesis. The probability of thermal damage to the osteocyte, which was calculated using the Arrhenius model, was negligible at a distance of at least 1 mm away from the cement–bone interface..
8. Mohammed Shurrab, Haidong Wang, Takanobu Fukunaga, Kosaku Kurata, Hiroshi Takamatsu, Feasibility of using apparent thermophysical properties to incorporate the effect of blood perfusion in estimation of temperature in tissues, Journal of Thermal Science and Technology, 10.1299/jtst.2017jtst0034, 12, 2, 2017.11, Estimation of temperature distribution in tissues and organs is critically important for treatments such as hyperthermia, radiofrequency ablation and cryosurgery which expose malignant tissue to extreme temperatures that are different from the physiological temperature. Commonly, the bioheat equation, instead of heat conduction equation, is used for estimation to incorporate the effect of blood perfusion, because the heat transfer in tissues is significantly affected by blood perfusion in addition to thermophysical properties of tissues. Nevertheless, in many cases, the rate of blood perfusion is not available for human tissues and organs. This study therefore aims to examine if we can use the normal heat conduction equation with apparent thermophysical properties to take the effect of blood perfusion into account. Feasibility was checked by comparing the results obtained from the heat conduction equation and the bioheat equation. The result indicated that the simulation with the apparent thermal conductivity or specific heat capacity does not agree well with the temperature distribution inside a tissue with blood perfusion. However, the apparent thermal conductivity was useful to estimate the size of growing ice ball produced during cryosurgery..
9. Kosaku Kurata, Takashi Yoshii, Yoshihiro Deguchi, Hiroshi Takamatsu, Raman microspectroscopic detection of thermal denaturation associated with irreversible electroporation, International Journal of Heat and Mass Transfer, 111, 163-170, 2017.08.
10. Shuto Yoshimatsu, Masahiro Yoshida, Kosaku Kurata, Hiroshi Takamatsu, Development of contact irreversible electroporation using a comb-shaped miniature electrode, Journal of Thermal Science and Technology, 10.1299/jtst.2017jtst0023, 12, 2, 2017.08, Irreversible electroporation (IRE) has been studied as a less invasive method for tumor treatment. Since the mechanism of the treatment is based on the fatal perforation of the cell membrane caused by an external electric field, a tumor can be ablated non-thermally if an appropriate electric field is selected. However, an electric field more than a few kV/cm is required to accomplish ablation. In this study, we aim to examine the feasibility of a comb-shaped miniature electrode for reducing the required voltage for IRE. The reduction of the applied voltage while maintaining the potential difference was realized by narrowing the gap between the electrodes. A 150-μm-wide miniature electrode with a 100-μm gap between its teeth was fabricated using photolithography. In the experiment, the electrode was contacted onto a tissue phantom consisting of fibroblasts cultured in agarose gel three-dimensionally. After the application of electric pulses, cell ablation depth was examined using fluorescent staining. The miniature electrode successfully ablated the cells at the surface of the tissue phantom by the application of 90 electric pulses at 100 V. The maximum and average ablation depth were 72.7 μm and 61.0 ± 11 μm, respectively, which was approximately 40 % of that estimated by the numerical analysis. Our study showed that the contact-IRE using a miniature electrode in the order of sub-millimeter does ablate the superficial cells of targeted tissues upon the application of electric pulses of less than 100 V; however, further studies are required to maximize the ablation depth under the constraint of limited applied voltage..
11. Haidong Wang, Shiqian Hu, Koji Takahashi, Xing Zhang, Hiroshi Takamatsu, Jie Chen, Experimental study of thermal rectification in suspended monolayer graphene, Nature communications, 10.1038/ncomms15843, 8, 2017.06, Thermal rectification is a fundamental phenomenon for active heat flow control. Significant thermal rectification is expected to exist in the asymmetric nanostructures, such as nanowires and thin films. As a one-atom-thick membrane, graphene has attracted much attention for realizing thermal rectification as shown by many molecular dynamics simulations. Here, we experimentally demonstrate thermal rectification in various asymmetric monolayer graphene nanostructures. A large thermal rectification factor of 26% is achieved in a defect-engineered monolayer graphene with nanopores on one side. A thermal rectification factor of 10% is achieved in a pristine monolayer graphene with nanoparticles deposited on one side or with a tapered width. The results indicate that the monolayer graphene has great potential to be used for designing high-performance thermal rectifiers for heat flow control and energy harvesting..
12. Wang H D, Takamatsu H, Zhang X, Width-dependent thermal conductivity of suspended single- layer graphene, Proceedings of 1st Asian Conference on Thermal Science (ACTS 2017), 2017.03.
13. Wang H D, Takahashi K, Takamatsu H, Zhang X, Highly sensitive charge mobility of suspended monolayer graphene, Proceedings of 6 th International Symposium on Micro and Nano Technology (ISMNT 2017), 2017.03.
14. Haidong Wang, Kosaku Kurata, Takanobu Fukunaga, Xing Zhang, Hiroshi Takamatsu, Width dependent intrinsic thermal conductivity of suspended monolayer graphene, International Journal of Heat and Mass Transfer, 105, 76-80, 2017.01.
15. H.D.Wang, X.Zhang, Takamatsu H, Ultraclean suspended monolayer graphene achieved by in situ current annealing, Nanotechnology, 28, 45706, 2017.01.
16. Haidong Wang, Dingshan Zheng, Xing Zhang, Hiroshi Takamatsu, Weida Hu, Benchmark characterization of the thermoelectric properties of individual single-crystalline CdS nanowires by a H-type sensor, RSC Advances, 10.1039/c7ra02734f, 7, 41, 25298-25304, 2017.01, A precision H-type sensor method has been developed to measure the thermoelectric performance of individual single-crystalline CdS nanowires for the first time. A nanomanipulation probe was used to directly pick up an individual nanowire from the array and place it on the sensor. Our method is generally applicable to any nanowire synthesized in either array or powder form. By simply changing the external electrical circuits, the Seebeck coefficient, thermal conductivity, and electrical conductivity have been measured on the same nanowire sample to ensure high accuracy and reliability. CdS nanowires have a large Seebeck coefficient over 300 μV K-1 due to their wide band gap, while their thermal conductivity is only one-tenth of that of the bulk material owing to the significant phonon-surface scattering. The figure of merit, ZT, of the CdS nanowire is 0.01 at 320 K, which is larger by two orders of magnitude than the value for a Bi2S3 nanowire, showing a trend of rapid increase above 300 K..
17. Haidong Wang, Kosaku Kurata, Takanobu Fukunaga, Koji Takahashi, Xing Zhang, Hiroshi Takamatsu, Effect of nanohole defect on the thermal conductivity of free-standing single-layer graphene, Proceedings of 11th Asian Thermophysical Properties Conference (ATPC 2016), 2016.10.
18. Mohammed Shurrab, Haidong Wang, Noriaki Kubo, Takanobu Fukunaga, Kosaku Kurata, Hiroshi Takamatsu, The cooling performance of a cryoprobe: Establishing guidelines for the safety margins in cryosurgery, International Journal of Refrigeration, 67, 308-318, 2016.07.
19. Haidong Wang, Kosaku Kurata, Takanobu Fukunaga, Hiroki Ago, Hiroshi Takamatsu, Xing Zhang, Tatsuya Ikuta, Koji Takahashi, Takashi Nishiyama, Yasuyuki Takata, Simultaneous measurement of electrical and thermal conductivities of suspended monolayer graphene, Journal of Applied Physics, 119, 244306 (6 pages), 2016.06.
20. Haidong Wang, Kosaku Kurata, Takanobu Fukunaga, Hiroshi Takamatsu, Xing Zhang, Tatsuya Ikuta, Koji Takahashi, Takashi Nishiyama, Hiroki Ago, Yasuyuki Takata, A simple method for fabricating free-standing large area fluorinated single-layer graphene with size-tunable nanopores, Carbon, 99, 564-570, 2016.04.
21. Kazuhiro Nishimura, DONG HAI WANG, Takanobu Fukunaga, Kosaku Kurata, Hiroshi Takamatsu, Measurement of in-plane thermal and electrical conductivities of thin film using a micro-beam sensor: A feasibility study using gold film, International Journal of Heat and Mass Transfer, 95, 727-734, 2016.04, A new method was proposed for measuring the in-plane thermal conductivity of thin films using a free-standing “micro-beam” metallic sensor. The sensor is heated in a vacuum with a direct current to induce temperature rise, which is determined from the electrical resistance of the sensor. The method consists of two protocols: measurement of a bare sensor and that after deposition of a sample film on its top surface. Based on the principle that the temperature rises of the sensor with and without a deposited film is different from each other because of the difference in the in-plane thermal conductance, the thermal conductivity of the sample film is determined by comparing the measured temperature rise with that obtained by a numerical analysis. In the present study, measurement of a 20-nm thick gold film was demonstrated by fabricating two platinum sensors that have different widths. The measured thermal conductivities of the platinum sensor and the gold film were significantly smaller than those of bulk materials. The relation between the thermal conductivity and the electrical conductivity was also discussed..
22. Kosaku Kurata, Hiroshi Takamatsu, Medical application of intense electric pulses for sustainable health, AIP Conference Proceedings, 1717, 020002 (7 pages), 2016.03.
23. Mohammed Shurrab, Haidong Wang, Noriaki Kubo, Takanobu Fukunaga, Kosaku Kurata, Hiroshi Takamatsu, A Reference for Cryosurgery using Two Parallel Cryoprobes: Simulation and Experiment using a Tissue Phantom, Low Temperature Medicine, 41, 2, 69-74, 2016.03.
24. Haidong Wang, Kosaku Kurata, Takanobu Fukunaga, Hiroshi Takamatsu, Xing Zhang, Tatsuya Ikuta, Koji Takahashi, Takashi Nishiyama, Hiroki Ago, Yasuyuki Takata, In-situ measurement of the heat transport in defect- engineered free-standing single-layer grapheme, Scientific Reports, 6, 21823, 2016.02.
25. Alan Twomey, Kosaku Kurata, Hiroshi Takamatsu, Alptekin Aksan, Microheterogeneity in frozen protein solutions, International Journal of Pharmaceutics, 487, 91-100, 2015.06.
26. Hideto Hirahara, Yutaka Nagare, Alan Twomey, Kosaku Kurata, Takanobu Fukunaga, Alptekin Aksan, Hiroshi Takamatsu, Observation of ice-solute interaction in freezing of trehalose and albumin solutions by using confocal Raman microscope equipped with directional solidification stage, Proceedings of the 15th International Heat Transfer Conference, IHTC15-8933, 14, 2014.08.
27. Hiroshi Takamatsu, Kosaku Kurata, Engineering approach to irreversible electroporation, Proceedings of the 15th International Heat Transfer Conference, IHTC15-KN05, 18, 2014.08, 本論文では,著者が過去数年にわたって行って来た不可逆エレクトロポレーションに関する研究のレビューを行った.不可逆エレクトロポレーションとは高電圧パルスを組織に印加し,細胞膜のみを破壊する新しい治療法である.この治療法に関して,通電に起因した組織の温度上昇の検出とシミュレーションによる温度上昇の予測,電圧印加条件に依存した細胞壊死範囲の実験による特定とシミュレーションとの比較など,工学的な見地からの検討を行った..
28. Kosaku Kurata, Seiji Nomura, Hiroshi Takamatsu, Three-dimensional analysis of irreversible electroporation: Estimation of thermal and non-thermal damage, International Journal of Heat and Mass Transfer, 72, 66-74, 2014.05.
29. Gang Zhao, Hiroshi Takamatsu, Xiaoming He, The effect of solution nonideality on modeling transmembrane water transport and diffusion-limited intracellular ice formation during cryopreservation, Journal of Applied Physics, 115, 144701, 13, 2014.04.
30. Syamsul Hadi, Mamoru Nishitani, Agung Tri Wijayanta, Takanobu Fukunaga, Kosaku Kurata, Hiroshi Takamatsu, Contact measurement of thermal conductivity and thermal diffusivity of solid materials: Experimental validation of feasibility with a prototype sensor, International Journal of Heat and Mass Transfer, 10.1016/j.ijheatmasstransfer.2013.09.068, 69, 256-263, 2014.02, 新しい治療法である不可逆エレクトロポレーションを表在性の腫瘍に適用する為の基礎研究として,生体ファントムを用いた実験およびシミュレーションを行った..
31. Hiroshi Takamatsu, Takanobu Fukunaga, Yuki Tanaka, Kosaku Kurata, Koji Takahashi, Micro-beam sensor for detection of thermal conductivity of gases and liquids, Sensors and Actuators A, 10.1016/j.sna.2013.11.019, 206, 10-16, 2014.02, 本論文は,新しく提案したビーム型のMEMSセンサを用いて液体及び気体試料の熱伝導率を測定する方法に関するものである.このセンサを用いると,原理的にはわずか1マイクロリットルの試料の熱伝導率を1ミリ秒程度で簡単に測定できる.本論文では,実際にセンサを試作して,測定が可能であることを実証した..
32. Kosaku Kurata, Ryo Ueno, Masahiro Matsushita, Takanobu Fukunaga, Hiroshi Takamatsu, Experimental and Analytical Studies on Contact Irreversible Electroporation for Superficial Tumor Treatment, Journal of Biomechanical Science and Engineering, 8, 4, 306-318, 2013.12, 本論文は,新しい低侵襲治療法である非熱的不可逆エレクトロポレーションを表在組織に適用する為の実験的,解析的研究である.不可逆エレクトロポレーションは,組織に穿刺した電極間に高電圧パルスを印加して細胞膜のみを破壊する治療法であるが,本研究では穿刺電極を使わない接触式エレクトロポレーションを提案し,数値解析とともに三次元培養モデル組織を用いた実験によりその実現可能性を示した..
33. Alan Twomey, Rebekah Less, Kosaku Kurata, Hiroshi Takamatsu, Alptekin Aksan, In Situ Spectroscopic Quantification of Protein–Ice Interactions, The Journal of Physical Chemistry B, 117, 26, 7889-7897, 2013.07.
34. Kosaku Kurata, Takashi Yoshii, Satoru Uchida, Takanobu Fukunaga, Hiroshi Takamatsu, Visualization of electroporation-induced temperature rise using temperature-sensitive ink, International Journal of Heat and Mass Transfer, 10.1016/j.ijheatmasstransfer.2012.07.038, 55, 23-24, 7207-7212, 2012.11, 本論文は,不可逆エレクトロポレーションで生じる可能性のある組織の瞬間的な温度上昇を初めて実験的に捉えた研究である.高電圧パルス印加による温度上昇は数十マイクロ秒程度であるため,通常の温度測定法では測定不可能である.そこで,本研究では,感温性のインクを使って温度上昇を検知する方法を開発し,生体ファントムの温度上昇の測定に成功した..
35. Takashi Kono, Yasunori Ayukawa, Yasuko Moriyama, Kosaku Kurata, Hiroshi Takamatsu, Kiyoshi Koyano , The effect of low-magnitude, high-frequency vibration stimuli on the bone healing of rat incisor extraction socket, Journal of Biomechanical Engineering, 134.0, 9.0, 091001(6 pages), 2012.09.
36. Syamsul Hadi, Mamoru Nishitani, Agung Tri Wijayanta, Kosaku Kurata, Hiroshi Takamatsu, Measurement of Thermal Conductivity and Thermal Diffusivity of Solid Materials Using a Novel Stamp Sensor: A Feasibility Study with Numerical Analysis, Journal of Thermal Science and Technology, 7.0, 4.0, 536-548, 2012.08.
37. Kosaku Kurata, Masahiro Matsushita, Takashi Yoshii, Takanobu Fukunaga, Hiroshi Takamatsu, Effect of Irreversible Electroporation on Three-Dimensional Cell Culture Model, The 34th Annual International Conference of the IEEE EMBS12, 2012.08.
38. Syamsul Hadi, Mamoru Nishitani, Agung Tri Wijayanta, Kosaku Kurata, Hiroshi Takamatsu, Measurement of Thermal Conductivity and Thermal Diffusivity of Solid Materials Using a Novel Stamp Sensor: A Feasibility Study with Numerical Analysis, Journal of Thermal Science and Technology, 7.0, 4.0, 536-548, 2012.08.
39. Gang Zhao, Kosaku Kurata, Hiroshi Takamatsu, Measurement of membrane hydraulic conductivity of bovine carotid artery endothelial cells using a perfusion microscope , Cryo-Letters, 33.0, 3.0, 232-240, 2012.05.
40. K. Kurata, H. Takamatsu, Effect of Hyperthermal Treatment on the Viability of Bone-Derived Cells, Journal of Biomechanical Science and Engineering, 6.0, 2.0, 101-113, 2011.06.
41. S. Nomura, K. Kurata, H. Takamatsu, Effect of Three-Dimensional Electric Field and Heat Conduction to Electrodes on the Temperature Rise

During Irreversible Electroporation, Proc. The 8th ASME-JSME Thermal Engineering Joint Conference, AJTEC2011-44214, 1-10., 2011.03.
42. H. Takamatsu, T. Tanaka, Y. Furuya, S. Uchida, K. Kurata, K. Takahashi, Preliminary Study of the Measurement of Thermal Conductivity of Fluids with a Micro-Beam MEMS Sensor, Proc. 9th Asian Thermophysical Properties Conference, Paper No. 109047, 2010.10, 本論文は,微量流体試料の熱伝導率が測定できるMEMSセンサの開発を目的として行ったものである.そして,マイクロビームセンサと名付けた長さが10ミクロン程度の微細なセンサを加熱すると,数百マイクロ秒で定常熱伝導状態に達することを数値計算により見出した.そして,定常に達した後のセンサ温度に及ぼす試料の熱伝導率の影響を明らかにし,新しい熱伝導率センサが開発可能であることを示した..
43. H. Takamatsu, Biotransport in Freezing of Cells, Proc. National Thermofluid Seminar, 2010.08.
44. H. Takamatsu, K. Inada, S. Uchida, K. Takahashi, M. Fujii, Feasibility Study of a Novel Technique for Measurement of Liquid Thermal Conductivity with a Micro Beam Sensor, International Journal of Thermophysics, 31.0, 41734.0, 888-899, 2010.05.
45. H. Takamatsu, K. Inada, T. Tanaka, S. Uchida, K. Takahashi, M. Fujii, Feasibility Study of a Novel Technique for Measurement of Liquid Thermal Conductivity with a Micro Beam Sensor, Proc. 17th Symposium on Thermophysical Properties, 2009.06.
46. T. Yoshimori, H. Takamatsu, 3-D measurement of osmotic dehydration of isolated and adhered PC-3 cells, Cryobiology, Vol. 58, No. 1, pp.52-61, 2009.02.
47. H. Takamatsu, S. Uchida, T. Matsuda, In situ harvesting of adhered target cells using thermoresponsive substrate under a microscope: Principle and instrumentation, J. Biotechnology, Vol. 134, No. 3-4, 297-304, 2008.04.
48. H. Takamatsu, Osmotic Injury and its Influence on Cell Damage during Freezing of Isolated Cells in Suspension, Cryobiology and Cryotechnology, 39-45 (2007)..
49. H. Takamatsu, Freezing of Cells; Role of Ice and Solutes in Cell Damage, Proc. ASME-JSME 2007 Thermal Eng. and Summer Heat Transfer Conf. (CD-ROM), HT2007-32250, 1-10, 2007.07.
50. K. Yoshida, S. Uchida, H. Takamatsu, M. Fujii, Feasibility Study of Noninvasive Measurement of Thermal Conductivity and Thermal Diffusivity for Biological Materials, Proc. ASME-JSME 2007 Thermal Eng. and Summer Heat Transfer Conf. (CD-ROM), HT2007-32740, 1-6, 2007.07.
51. S. Uchida, K. Yoshida, H. Takamatsu, X. Zhang, M. Fujii, Noninvasive Measurement of Thermal Conductivity and Thermal Diffusivity of Biological Materials (Preliminary Examination of Heat Transfer Models and Measurement Accuracy), Transactions of JSME, Ser. B, 72, 723, 2774-2779 (2006)..
52. H. Takamatsu, S Uchida, T. Matsuda, A Method for In-Situ Targeting and Harvesting of Cultured Cells Under a Microscope, Proc. 2006 Int. Symp. Micro-Nanomechatronics and Human Science, 160-163, 2006.11.
53. H. Takamatsu, S. Zawlodzka, Contribution of Extracellular Ice Formation and the Solution Effects to the Freezing Injury of PC-3 Cells Suspended in NaCl Solutions, Cryobiology, 53, 1, 1-11, 2006.08.
54. S. Zawlodzka, H. Takamatsu, Osmotic Injury of PC-3 Cells by Hypertonic NaCl Solutions at Temperatures above 0°C, Cryobiology, 10.1016/j.cryobiol.2004.10.004, 50.0, 1.0, 50, 1, 58-70, 2005.02.
55. H. Takamatsu, R. Takeya, S. Naito, H. Sumimoto, On the Mechanism of Cell Lysis by Deformation, J. Biomech., 10.1016/j.jbiomech.2004.03.011, 38.0, 1.0, 38, 1, 117-124, 2005.01.
56. H. Takamatsu, Y. Komori, S. Zawlodzka, M. Fujii, Quantitative Examination of a Perfusion Microscope for the Study of Osmotic Response of Cells, J. Biomech. Eng., 10.1115/1.1784474, 126.0, 4.0, 126, 4, 402-409, 2004.08.
57. H. Honda, N. Takata, H. Takamatsu, J.S. Kim, K. Usami, Effect of Fin Geometry on Condensation of R407C in a Staggered Bundle of Horizontal Finned Tubes, J. Heat Transfer, 10.1151/1.1560153, 125.0, 4.0, 125, 653-660, 2003.08.
58. H. Takamatsu, H. Yamashiro, N. Takata, H. Honda, Vapor Absorption by LiBr Aqueous Solution in Vertical Smooth Tubes, Int. J. Refrig., 10.1016/S0140-7007(03)00038-0, 26.0, 6.0, 26, 659-666, 2003.07.
59. H. Honda, H. Takamatsu, J.J. Wei, Enhanced Boiling Heat Transfer from Silicon Chips with Micro-Pin Fins Immersed in FC-72, J. Enhanced Heat Transfer, 10.0, 2.0, 10, 2, 211-223, 2003.04.
60. H. Honda, J.J. Wei, H. Takamatsu, H. Yamashiro, Heat Transfer Characteristics of a Natural Circulation Liquid Cooling System for Electronic Components, Proc. 6th ASME/JSME Therm. Eng. Conf., TED-AJ03-122, 8.0, 2003.03.
61. H. Takamatsu, N. Kumagae, Survival of Biological Cells Deformed in a Narrow Gap, J. Biomech. Eng., 10.1115/1.1516197, 124.0, 6.0, 124, 6, 780-783, 2002.12.
62. H. Takamatsu, H. Yamashiro, N. Takata, T. Nakamaya, H. Honda, Absorption of Water Vapor by LiBr Aqueous Solution in a Vertical Smooth Tube, Trans. JSRAE.
63. H. Honda, J.J. Wei, H. Takamatsu, Effect of Surface Microstructure on Boling Heat Transfer from Silicon Chips Immersed in FC-72, Therm. Sci. Eng., 10, 5, 9-17, 2002.09.
64. H. Takamatsu, S. Kawahara, T. Kurokawa, H. Honda, Osmotin Volume Change and Cellular Injury by Hypertonic Sodium Choloride Solution, Trans. JSME, Ser. B.
65. H. Honda, H. Takamatsu, J.J. Wei, Effect of the Size of Micro-Pin-Fin on Boiling Heat Transfer from Silicon Chips Immersed in FC-72, Trans. JSME, Ser. B.
66. H. Honda, H. Takamatsu, J.J. Wei, Effect of the Size of Micro-Pin-Fin on Boiling Heat Transfer from Silicon Chips Immersed in FC-72, Proc. 12th Int. Heat Transfer Conf., 75-80, 2002.08.
67. H. Honda, H. Takamatsu, J.J. Wei, Boiling of FC-72 on Silicon Chips with Micro-Pin-Fins and Submicron-Scale Roughness, Trans. JSME, Ser. B.
68. H. Honda, N. Takata, H. Takamatsu, J. -S. Kim, K. Usami, Condensation of Downward-flowing HFC134a in a Staggered Bundle of Horizontal Finned Tubes: Effect of Fin Geometry, Int. J. Refrigeration, 10.1016/S0140-7007(01)00023-8, 25.0, 1.0, 25, 3-10, 2002.01.
69. H. Takamatsu, H. Yamashiro, N. Takata, H. Honda, K. Kasatsugu, Absorption of Water Vapor by LiBr Aqueous Solution in a Vertical Smooth Tube, Proc. IIR Conf. Commission B1, Thermopyhsical Properties and Transfer Processes of New Refrigerants, 2001.10.
70. H. Honda, H. Takamatsu, J. J. Wei, Effect of the Size of Micro-Pin-Fin on Boiling Heat Transfer from Silicon Chips Immersed in FC-72, Therm. Sci. Eng., 9, 4, 27-28, 2001.06.
71. H. Takamatsu, N. Kumagae, B. Rubinsky, The Effect of Temperature on the Viability of Deformed Cells, ASME HTD-VOl. 368/BED-Vol. 47, Advances in Heat and Mass Transfer in Biotechnology 2000, 55-58, 2000.11.
72. H. Honda, N. Takata, H. Takamatsu, J. -S. Kim, K. Usami, Effect of Fin Geometry on Condensation of R134a in a Staggered Bundle of Horizontal Finned Tubes, Proc. 4th JSME/KSME Therm. Eng. Conf., 1, 1-10, 2000.10.
73. H. Honda, H. Yamashiro, H. Takamatsu, Stability of Vapor Film in Subcooled Film Boiling on a Sphere, Proc. 34th Nat. Heat Transfer Conf., 41649.0, 2000.08.
74. H. Honda, N. Takata, H. Takamatsu, J.-S. Kim, K. Usami, Effect of Fin Geometry on Condensation of R134a in a Staggered Bundle of Horizontal Finned Tubes, Trans. JSRAE.
75. H. Honda, N. Takata, H. Takamatsu, J.-S. Kim, K. Usami, Effect of Fin Geometry on Condensation of Zeotropic Refrigerant Mixture R407C in a Staggered Bundle of Horizontal Finned Tubes, Trans. JSRAE.
76. H. Honda, H. Takamatsu, N. Takata, Experimental Measurements for Condensation of Downward-Flowing R123/R134a in a Staggered Bundle of Horizontal Low-Finned Tubes with Four Geometries, Int. J. Refrig., 10.1016/S0140-7007(99)00026-2, 22.0, 8.0, 22, 8, 615-624, 1999.12.
77. H. Takamatsu, M. Fujii, H. Honda, H. Uchiyama, Stability of Annular Liquid Film in Microgravity, Microgravity Science and Technology, 12.0, 1.0, 12, 1, 2-8, 1999.12.
78. H. Takamatsu, B. Rubinsky, Measurement of the Viability of Cells Compressed by Two Parallel Plates, Trans. JSME, Ser. B.
79. H. Takamatsu, B. Rubinsky, Viability of Deformed Cells, Cryobiology, 10.1006/cryo.1999.2207, 39.0, 3.0, 39, 3, 243-251, 1999.11.
80. H. Honda, H. Yamashiro, H. Takamatsu, Stability of Vapor Film in Subcooled Film Boiling on a Sphere, Therm. Sci. Eng..
81. H. Honda, H. Yamasho, H. Takamatsu, K. Tanaka, Effect of the Behavior of Generated Vapor on the Minimum-Heat-Flux Point During Rapid Quenching of a Thin Horizontal Wire, Trans. JSME, Ser. B.
82. H. Honda, H. Takamatsu, N. Takata, M. Tawaraya, Condensation of Downward-Flowing R407C in a Staggered Bundle of Horizontal Low-Finned Tubes, Proc. 1st Int. Conf. Engineering Thermophysics(ICET '99), 404-411, 1999.08.
83. H. Kubo, H. Takamatsu, H. Honda, Effect of Size and Number Density of Micro-Reentrant Cavities on Boiling Heat Transfer from a Silicon Chip Immersed in Degassed and Gas-Dissolved FC-72, J. Enhanced Heat Transfer, 6, 2-4, 151-160, 1999.05.
84. H. Honda, H. Takamatsu, N. Takata, Condensation of Downward-Flowing Zeotropic Mixture HCFC-123/HFC-134a on a Staggered Bundle of Horizontal Low-Finned Tubes, J. Heat Transfer, 10.1115/1.2825993, 121.0, 2.0, 121, 2, 405-412, 1999.05.
85. H. Honda, H. Yamashiro, H. Takamatsu, Effect of the Behavior of Generated Vapor on the Minimum-Heat-Flux Point During Rapid Quenching of a Thin Horizontal Wire, Proc. 5th ASME/JSME Therm. Eng. Conf., AJTE99-6366, 1999.03.
86. K.-S. Cho, H. Honda, H. Takamatsu, H. Nakahama, Effect of Water Temperature on the Cooling Characteristics and Non-Crystallinity of FeSi7.5B15 Wires Rapidly Solidified by the In-Rotating-Water Spinning Method, Proc. 5th ASME/JSME Therm. Eng. Conf., AJTE99-6466, 1999.03.
87. H. Honda, H. Takamatsu, H. Yamashiro, Stability of Vapor film in Subcooled Forced-Convection Film Boiling on a Horizontal Cylinder, Trans. JSME, Ser. B.
88. K. -S. Cho, H. Takamatsu, H. Honda, Effect of Water Temperature on the Non-Crystallinity of FeSi7.5B15(at%) Wires Produced by the In-Ratating-Water Spinning Method, J. Japan Instute of Metals.
89. S.-R. Tian, H. Takamatsu, H. Honda, Experimental Study on the Immersion Cooling of an Upward-Facing Multichip Module with an Opposing Condensing Surface, Heat Transfer Jpn. Res., 27, 7, 497-508, 1998.11.
90. H. Honda, H. Takamatsu, N. Takata, Effect of Fin Geometry on Condensation of Downward-Flowing R123/R134a in a Staggered Bundle of Horizontal Low-Finned, Proc. Eurotherm Seminar 62, 456-465, 1998.11.
91. K.-S. Cho, H. Takamatsu, H. Honda, K. Morinaga, Effect of Spinning condition on Non-Crystallinity of Fe77.5Si7.5B15(at%) Wire Produced by In-Rotating-Water Spinning Method, J. Japan Instute of Metals.
92. H. Honda, H. Takamatsu, H. Yamashiro, Stability of Vapor Film in Subcooled Forced-Convection Film Boiling on a Horizontal Cylinder, Proc. 11th Int. Heat Transfer Conf., 2, 297-302, 1998.08.
93. H. Honda, H. Takamatsu, N. Takata, Condensation of Downward-Flowing Zeotropic Mixture HCFC-123/HFC-134a on a Staggered Bundle of Horizontal Low-Finned Tubes-Effect of Fin Geometry-, Trans. JSRAE.
94. H. Takamatsu, H. Kubo, H. Honda, Immersion Cooling of a Simulated Microelectronic Chip with Artificial Re-entrant Cavities, Trans. JSME, Ser. B.
95. H. Takamatsu, M. Fujii, H. Honda, H. Uchiyama, Stability of Annular Liquid Film in Microgravity, Trans. JSME, Ser. B.
96. H. Honda, H. Takamatsu, N. Takata, Condensation of Downward-Flowing Zeotropic Mixture HCFC-123/HFC-134a on a Staggered Bundle of Horizontal Low-Finned Tubes, Trans. JSRAE.
97. K.-S. Cho, H. Takamatsu, H. Honda, Effect of Spinning Conditions on the Shape of Metallic Wires Produced by the In-Rotating-Water Spinning Method, J. Japan Instute of Metals.
98. H. Yamashiro, H. Takamatsu, H. Honda, Enhancement of Cooling Rate During Rapid Quenching of a Thin Wire by Ultrasonic Vibration, Heat Transfer Jpn. Res., 27, 1, 16-30, 1998.02.
99. H. Yamashiro, H. Takamatsu, H. Honda, Effect of Ultrasonic Vibration on Transient Boiling Heat Transfer During Rapid Quenching of a Thin Wire in Water, J. Heat Transfer, 10.1115/1.2830055, 120.0, 1.0, 120, 1, 282-286, 1998.02.
100. K.-S. Cho, H. Takamatsu, H. Honda, Effect of Spinning Condition on the Cooling Characteristics and Shape of FeSi77.5B15 Wires Rapidly Solidified by the In-Rotating-Water Spinning Method, Heat Transfer Jpn. Res., 27, 1, 31-42, 1998.02.