Kyushu University Academic Staff Educational and Research Activities Database
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Sumiko Watanabe Last modified date:2022.06.27

Lecturer / Medical Technology Course
Department of Health Sciences
Faculty of Medical Sciences

Graduate School

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Philosophy
Field of Specialization
Research Interests
  • Development of cytological support system with Deep Learning
    keyword : morphometry、nuclear chromatin、cytoplasmic change、morphology
  • Investigation of the mechanism of BCG for Bladder cancer
    keyword : DNA、pKi67、p21, p27, cancer cell、cytology、chromatin structure, cell cycle, Lamin
    1992.06DNA、pKi67、cancer cell、cytology、chromatin stracture.
  • Development of cytological morphometry for cancer cells
    keyword : morphometry、nuclear chromatin、cytoplasmic change、morphology
  • Cancer cell Morphology
    keyword : DNA、pKi67、cancer cell、cytology、chromatin structure, cell cycle, Lamin
    1992.06DNA、pKi67、cancer cell、cytology、chromatin stracture.
Current and Past Project
  • Morphologic Diagnosis of Atypical Glandular Lesion using a Conventional Pap Smear from GOG-171 Patients
Academic Activities
1. Sumiko Watanabe, Report on the “scientific progress and woman scientists” course, 基幹教育紀要 (Vol. 1), 2015.04, In the first term of 2014, I taught a course entitled “Scientific Progress and Woman Scientists” as one of General Subjects
at Faculty of Arts and Science (FAC), Kyushu University. Thirty-eight students attended: 25 freshmen, 11 sophomores, a
junior and a senior, belonging to nine different undergraduate schools. The purposes of taking this course were to know
and understand woman scientists through their experiences and to obtain some of the wisdom they had gained. The course
was designed in accord with the study perspective of FAC. In each of the course’s 15 lessons, I introduced a woman
scientist and her work, her co-workers and her affiliations, using slides and other materials. I introduced 11 woman
scientists, 20 universities and nine institutions of eight countries during the course. As an evaluation of the course, I had
the students complete two questionnaire surveys and write a report about woman scientists. In the questionnaire responses
and reports, I found some interesting issues. First, the students’ degree of recognition of women scientists was lower than
that of male scientists. Second, the woman scientist that the students selected as the most interesting scientist was Rosalind
Franklin. Third, it was notable that many students felt that spiritual strength was most important for success as a scientist.
Some students stated that they selected this course while thinking of their future as a scientist. I thus suggest that this
course should be further developed to provide not only knowledge but also encouragement to young scientists..
2. Past, Present and Future of Cytology.
1. 渡邊壽美子, 加来恒壽, 西村和徳, 植村瑞紀, 上原俊貴, 杉島節夫, 加藤聖子, 小田義直,, 岩坂剛:, 子宮頸部腺系病変における細胞像の数量的解析-AGCに注目して-, 日本臨床細胞学会九州連合会雑誌, 2021.08.
2. Sumiko Watanabe, Shota Yamaguchi, Naoto Fujii, Natsuki Eguchi, Hitoshi Katsuta, Setsuo Sugishima, Tsuyoshi Iwasaka, Tsunehisa Kaku:, Nuclear Co-expression of p21 and p27 Induced Effective Cell-cycle Arrest in T24 Cells Treated with BCG., Cytotechnology, 10.1007/s10616-018-0278-5, 2018.09.
3. Kazunori Nishimura, Sumiko Watanabe, Ryo Hayashida, Setsuo Sugishima, Tsuyoshi Iwasaka, Tsunehisa Kaku, Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation, Cytotechnology, 10.1007/s10616-015-9869-6, 2015.04, Many cytological studies have reported
that the numbers of binucleated cells were elevated in
various tumors. However, binucleated cells are observed
in not only malignant tumors but also normal
tissues. Thus, the clinical significance of binucleated
cells is controversial. Here we attempted to elucidate
the characteristics of binucleated HeLa cells using
time-lapse microscopy. To examine the frequency,
viability, proliferation, and formation mechanism of
binucleated cells, we grew HeLa cells on chamber
slides and tissue culture dishes in DMEM supplemented
with (10, 3, 1 and 0.5 % media) and without
fetal bovine serum (0 % medium). The proliferation
was evaluated by the medium improvement examination
(cultured for 2 more days in 10% medium after
culturing in 0% medium; starvation). In the 0 %
medium, 150 binucleated cells were formed by
cytokinesis failure. There were significantly more
binucleated cells in the 0 % medium than in the 10, 3,
1 and 0.5 % media. About twice the number of
binucleated cells underwent mitosis in the improvement
examinations than in the serum-free examination.
We found here that starvation induced the
binucleation of HeLa cells and that some binucleated
cells can reproduce. These findings might be helpful
for understanding binucleated cells in tumors..
4. Micropapillary variant of urotherial carcinoma of the urinary bladder-a case report-.
5. Cytological diagnosis of endometrial hyperplasia-Trials for the distinction of endometrial hyperplasias.
6. 渡辺寿美子、岩坂剛、横山正俊、内山倫子、加来恒寿、松山敏剛, Analysis of Nuclear Chromatin Distribution in Cervical Glandular Abnormalities, Acta Cytologica, Vol.48 No.4, July-August 2004, 2004.07.
1. Sumiko Watanabe, Tsunehisa Kaku, Maki Kusaba, Natsuki Eguchi, Kazunori Nishimura, Setsuko Murata, Setsuo Sugishima, Tsuyoshi Iwasaka, Formation Mechanism of Binucleated HeLa Cells, 38th European Congress of Cytology 2014, 2014.09, Introduction:
Many cytological studies have reported an increase in the numbers of binucleated cells in various tumors. We found that the numbers of binucleated HeLa cells in a serum-free condition was significantly higher than that in a conventional condition. Two reports have revealed different mechanisms for binucleated cell formation: ‘cell–cell fusion’ and ‘abnormal cytokinesis’. Here we attempted to elucidate the formation mechanism of binucleated HeLa cells using CellTracker™.
Materials and Methods:
We grew HeLa cells on chamber slides for 4 days in DMEM supplemented with fetal bovine serum (10% medium). After that, we removed the 10% medium and cultured HeLa cells in DMEM without fetal bovine serum (0% medium) including CellTracker™ for 45 minutes. Then co-culture 2 kinds cells treated with the two different CellTracker™ probes (Red or Green) in 0% medium for 3 days. We observed the cytoplasmic staining condition of binecleated HeLa cells by confocal laser scanning microscopy (CLSM).
We observed 54 binucleated HeLa cells. The ratio of cytoplasmic stained with red, green and yellow (means stained with both) was 40.7%, 57.4% and 1.9%, respectively. The ratio of single-color binucleated cells was 98.1%. If the formation mechanism is cell–cell fusion, cytoplasms of binucleated cells might be stained with red, green and yellow and each theoretical frequency of binucleated cells was about 25%, 25% and 50%, respectively. There were large discrepancies in experimental and theoretical values of cell–cell fusion.
Binucleated HeLa cells might result from abnormal cytokinesis.
2. 渡邊 壽美子, 加来 恒壽, 大喜 雅文, 田宮 貞史, 杉島 節夫, 村田節子, 大石 善丈, 横山正俊, 柏村正道, 柏村賀子, 岩坂剛, Correlation between nuclear chromatin pattern and cell cycle, 18th International Congress Of Cytology, 2013.05, Objectives:
The chromatin pattern of nuclei is one of the most important criteria for cytological diagnosis. However many terms like fine granular, coarse granular, opaque and so on, very few reports have actually referred to the need to clarify the definition of chromatin pattern. In the present study, we quantitatively analyzed nuclear chromatin distribution in 3 cancer cell lines and examined the relationship between the chromatin distribution pattern and the cell cycle.

Materials and Methods:
We used three cultured cell lines; HeLa (moderate differentiated cervical adenocarcinoma), TMCC-1 (undifferentiated cervical adenicarcinoma) and T-24 (high grade urothelial carcinoma). Cells were cultured in Dulbecco’s modified Eagle’s medium: DMEM supplemented with 10% fetal calf serum and 1x antibiotic-antimycotic solution at 37oC in 5 % CO2 for 3,5,7 and 10 days on chamber slides. Three hundreds cells were selected at random in each slide. Cell images stained with the Papanicolaou method were acquired by a digital camera attached to a microscope and saved in a computer in JPEG format. Chromatin patterns were divided into 4 types; fine granular (F), Medium granular (M), coarse granular (C) and opaque (O). DNA contents were measured for the cell cycle analysis with flowcytometer.

Chromatin pattern compositions of the following days were similar between 3 cells. The ratio of C type was increasing (average of 3 cells; 1.7%, 3.0%, 11.0% and 10.3% was day 3, day 5, day 7 and day 10, respectively), while F type was decreasing (average of 3 cells; 69.0% 56.0%, 26.0% and 6.7% was day 3, day 5, day 7 and day 10, respectively). O type was appeared just only day 10. The ratio of G1 of 3 cells was increasing (average of 3 cells; 54.5% 68.1%, 72.7% and 74.5% was day 3, day 5, day 7 and day 10, respectively).

It is suggested that there is a significant correlation between the nuclear chromatin pattern and the ratio of G1 phase of cell cycle.
3. The significance of NUcleoli and Chromatin distribution of glandular cells in cervical adenocarcinoma.
4. Comparison the condition of Papanicolaou stain with the DNA content in voided-urine cytology.
5. Significance of Nucleoli in cervical glandular cells : the second report.
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