| 1. |
細胞内シグナル酵素応答型ポリマーによる遺伝子発現制御. |
| 2. |
ペプチドアレイを用いた表面プラズモン共鳴(SPR)イメージングによる網羅的なOn‐chipリン酸化解析への展開. |
| 3. |
プロテインキナーゼ活性検出のための蛍光プローブの作製と機能評価. |
| 4. |
Gene delivery system use of sonoporation with gene carriers responding to cellular signals. |
| 5. |
クリックケミストリーによるポリマーへの修飾. |
| 6. |
がん細胞内の異常シグナルを利用した遺伝子デリバリーシステム. |
| 7. |
NIIDOME Takuro, OHGA Akira, NIIDOME Yasuro, MORI Takeshi, KATAYAMA Yoshiki, Development of Gold Nanorods That Accumulatate into Tumor, Peptide science : proceedings of the ... Japanese Peptide Symposium, Vol.2008, pp.113-114, 2009.03. |
| 8. |
ペプチド複合体を用いるプロテインキナーゼ計測用蛍光分子プローブ. |
| 9. |
ペプチドグラフト型LPEIを用いたD‐RECS法における癌特異性の向上. |
| 10. |
がん診断を目指した蛍光物質含有超音波造影剤の開発. |
| 11. |
がん細胞特異的に遺伝子を活性化するシグナル応答型遺伝子キャリヤー. |
| 12. |
The effect of PEG grafted on gold nanorods and their injection dose on biodistribution in tumor-bearing mice
Gold nanorods have strong surface plasmon band in the near-infrared region, at which light penetrates deeply into tissues, and convert the absorbed light energy into heat. Therefore, gold nanorods are expected to act as an effective contrast agent for in vivo bioimaging and as a photosensitizer for photothermal therapy. In order to efficiently achieve these applications without side effects, gold nanorods should be selectively accumulated in the target organ. In this study, we optimized the length of the poly (ethylene glycol) (PEG) chain to stabilize the PEG-modified gold nanorods in blood circulation, and investigated the effects of PEG grafting level and injection dose on the biodistribution and enhanced permeability and retention (EPR) effect after intravenous injection into mice. PEG 5,000 - , PEG 10,000 - and PEG 20,000 - modified gold nanorods showed higher blood circulation stability compared with PEG 2,000 - modifed gold nanorods. Higher PEG grafting levels were advantageous for the reticuloendothelial system (RES) avoidance and suppression of aggregation of the gold nanorods in the blood circulation. Modification with a PEG 5,000 : gold molar ratio of 1.5 was sufficient to show both prolonged circulation and the EPR effect. When the injection dose was increased above 39.0 μg of gold, the RES uptake in the liver was saturated and surplus gold nanorods were distributed to other tissues, especially the spleen and tumor. This information is important key to provide the successful application of gold nanorods in the field of nanomedicine. © 2010 Materials Research Society.. |
| 13. |
The combination of drug or gene delivery system responding to cellular signals (D-RECS) and sonoporation system for effective and safe gene delivery
We have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals. © 2010 Materials Research Society.. |
| 14. |
高分子‐ペプチド複合体による遺伝子の転写制御. |
| 15. |
細胞内シグナルに応答したsiRNAデリバリーシステムの開発. |
| 16. |
ポリマー/ペプチドコンジュゲートを用いるがんイメージング剤―前立腺がん特異的プロテアーゼ蛍光プローブ. |
| 17. |
高分子型蛍光プローブによるプロテインキナーゼの活性検出. |
| 18. |
細胞内シグナル応答型遺伝子医薬送達システムの複合体安定化の試み. |
