Kyushu University Academic Staff Educational and Research Activities Database
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Hirofumi Ichinose Last modified date:2023.11.27

Associate Professor / Biomaterial Science
Department of Agro-environmental Sciences
Faculty of Agriculture

Graduate School
Undergraduate School

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Bioengineering, Basidiomycetes
Total Priod of education and research career in the foreign country
Research Interests
  • Biotechnology of Basidiomycetes
    keyword : Basidiomycetes, Cytochrome P450
Academic Activities
1. Hirofumi Ichinose, Cytochrome P450 of wood-rotting basidiomycetes and biotechnological applications, Biotechnology and Applied Biochemistry. 2013;60:71-81., 2013.01.
2. Ichinose Hirofumi, Molecular and functional diversity of fungal cytochrome P450s, Biol Pharm Bull. 2012;35(6):833-7., 2012.06.
1. Natsuki Masunaga, Takuya Kitaoka, Hirofumi Ichinose, Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon, Microbial Biotechnology, 10.1111/1751-7915.14204, 16, 3, 632-644, 2023.03.
2. Dani Permana, Ksenia Niesel, Mark James Ford, Hirofumi Ichinose, Latent Functions and Applications of Cytochrome P450 Monooxygenases from Thamnidium elegans: A Novel Biocatalyst for 14α-Hydroxylation of Testosterone, ACS Omega, doi: 10.1021/acsomega.2c00430, 7, 13932-13941, 2022.04.
3. Hirofumi Ichinose, Shota Ukeba, Takuya Kitaoka, Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene, Enzyme Microb Technol, doi: 10.1016/j.enzmictec.2022.110037, 158, 110037, 2022.08.
4. Diego M. Mendoza, Hirofumi Ichinose, Kojo T. Konadu, Keiko Sasaki, Degradation of powder activated carbon by lactase-mediator system: Model experiments for the improvement of gold recovery from carbonaceous gold ore, JournalJournal of Environmental Chemical Engineering, doi: 10.1016/j.jece.2021.106375, 9, 6, 106375, 2021.12.
5. Toshio Mori, Haruka Ohno, Hirofumi Ichinose, Hirokazu Kawagishi, Hirofumi Hirai, White-rot fungus Phanerochaete chrysosporium metabolizes chloropyridinyl-type neonicotinoid insecticides by an N-dealkylation reaction catalyzed by two cytochrome P450s, J Hazard Mater, doi: 10.1016/j.jhazmat.2020.123831, 402, 123831, 2021.01.
6. Mayumi Hatakeyama, Daisuke Ryuno, Shingo Yokota, Hirofumi Ichinose, Takuya Kitaoka, One-step synthesis of cellooligomer-conjugated gold nanoparticles in a water-in-oil emulsion system and their application in biological sensing, Colloids Surf B Biointerfaces, doi: 10.1016/j.colsurfb.2019.02.051, 178, 74-79, 2019.06.
7. Jianqiao Wang, Haruka Ohno, Yuuri Ide, Hirofumi Ichinose, Toshio Mori, Hirokazu Kawagishi, Hirofumi Hirai, Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium, J Hazard Mater, doi: 10.1016/j.jhazmat.2019.03.042, 371, 494-498, 2019.06.
8. Mayumi Hatakeyama, Fumi Nakada, Hirofumi Ichinose, Takuya Kitaoka, Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose, Colloids Surf B Biointerfaces, doi: 10.1016/j.colsurfb.2018.12.039, 175, 517-522, 2019.03.
9. Kiyota Sakai, Fumiko Matsuzaki, Lisa Wise, Yu Sakai, Sadanari Jindou, Hirofumi Ichinose, Naoki Takaya, Masashi Kato, Hiroyuki Wariishi, Motoyuki Shimizu, Biochemical Characterization of CYP505D6, a Self-Sufficient Cytochrome P450 from the White-Rot Fungus Phanerochaete chrysosporium, Appl Environ Microbiol, doi: 10.1128/AEM.01091-18, 84, 22, e01091, 2018.10.
10. Hirofumi Ichinose, Takuya Kitaoka, Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis: semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism, Microbial Biotechnology, 10.1111/1751-7915.13304, 11, 952-965, 2018.07.
11. Hatakeyama Mayumi, Ichinose Hirofumi, Takuya Kitaoka, Impacts of amino acid substitutions in fungal cytochrome P450 monooxygenase (CYP57B3) on the effective production of 3'-hydroxygenistein, FEMS Microbiology Letters,, 364, 11, FEMS Microbiology Letters, Volume 364, Issue 11, 15 June 2017, fnx107, 2017.05.
12. Lili Jia, Kosuke Minamihata, Hirofumi Ichinose, Kouhei Tsumoto, Noriho Kamiya , Polymeric SpyCatcher Scaffold Enables Bioconjugation in a Ratio-Controllable Manner, Biotechnol J, doi: 10.1002/biot.201700195, 12, 12, 2017.12.
13. 一瀬博文, 畠山真由美, 山内幸代, Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli, J Biosci Bioeng, doi: 10.1016/j.jbiosc.2015.01.019., 120, 2, 268-274, J Biosci Bioeng. 2015 Sep;120(3):268-74. doi: 10.1016/j.jbiosc.2015.01.019., 2015.09.
14. Pornthida Poosala, Hirofumi Ichinose, Takuya Kitaoka, Spatial Geometries of Self-Assembled Chitohexaose Monolayers Regulate Myoblast Fusion, Int J Mol Sci, doi: 10.3390/ijms17050686, 17, 5, 686, 2016.05.
15. Ryuya Fujii, Takahiro Ugai, Hirofumi Ichinose, Mayumi Hatakeyama, Takuto Kosaki, Katsuya Gomi, Isao Fujii, Atsushi Minami, Hideaki Oikawa , Reconstitution of biosynthetic machinery of fungal polyketides: unexpected oxidations of biosynthetic intermediates by expression host, Biosci Biotechnol Biochem, doi: 10.1080/09168451.2015.1104234, 80, 3, 426-431, 2016.03.
16. Hatakeyama Mayumi, Ichinose Hirofumi, Takuya Kitaoka, Heterologous expression of fungal cytochromes P450 (CYP5136A1 and CYP5136A3) from the white-rot basidiomycete Phanerochaete chrysosporium: functionalization with cytochrome b5 in Escherichia coli, Enzyme and Microbial Technology, DOI: 10.1016/j.enzmictec.2016.03.004, 89, 2, 7-14, Enzyme and Microbial Technology, 89(-), 7-14 (2016), 2015.09.
17. Hirofumi Ichinose, Hiroyuki Wariishi, High-level heterologous expression of fungal cytochrome P450s in Escherichia coli, Biochem Biophys Res Commun, 438, 289-294, 2013.09.
18. Ide M, Ichinose H, Wariishi H., Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta, Arch Microbiol, 194, 243-253, 2012.04.
19. Nakamura T, Ichinose H, Wariishi H., Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds., Biodegradation, 23, 343-350, 2012.04.
20. Ichinose H, Wariishi H., Heterologous expression and mechanistic investigation of a fungal cytochrome P450 (CYP5150A2): Involvement of alternative redox partners., Arch Biochem Biophys, 518, 8-15, 2012.01.
21. Nazmul Hussain Nazir KHM, Ichinose H, Wariishi H., Construction and Application of a Functional Library of Cytochrome P450 Monooxygenases from the Filamentous Fungus Aspergillus oryzae, Appl Environ Microbiol, 77, 3147-3150, 2011.05.
1. Hirofumi Ichinose, Functional diversity and applications of fungal cytochromes P450, Korea-Japan smart biodesign workshop: technology exchange for green biotechnology, 2014.01.
2. Mayumi Hatakeyama, Hirofumi Ichinose, Hiroyuki Wariishi, Overexpression of multifunctional cytochrome P450s (CYP5136A1 & CYP5136A3) from white-rot fungi, 18th International conference on cytochrome P450, Biochemistry, Biophysics and Biotechnology, 2013.06.
3. Hirofumi Ichinose, A large-scale screening of heterologous expression of fungal cytochrome P450, 18th International conference on cytochrome P450, Biochemistry, Biophysics and Biotechnology, 2013.06.
Educational Activities
I am in charge of lectures on forest science and biotechnology. I strive to increase basic knowledge of student in these areas. I give specialized knowledge for students by providing opportunities to have discussion on cutting-edge research topics in seminars. In addition, I aim to improve presentation skills of students through practice.