Kyushu University [Kumamaru Wataru (Lecturer) Kyushu University Hospital, Maxill Ofacial Surgery]

Kumamaru Wataru

Last modified date：2020.01.09

Lecturer / Department of Oral and Maxillofacial Surgery, Graduate School of Dental Science, Kyushu University
Maxill Ofacial Surgery
Kyushu University Hospital

1 Research Interests

2 Academic Activities

2.1 Books

2.2 Papers

3 Membership in Academic Society

4 Educational Activities

E-Mail

Phone

092-642-6452

Fax

092-642-6392

Academic Degree

A study on tumor-specific cytotoxic T lymphocytes in patients with oral squamous cell carcinoma

Country of degree conferring institution (Overseas)

Field of Specialization

oral surgery

Total Priod of education and research career in the foreign country

00years00months

Outline Activities

Controlling metastatic lesions is an important part of improving cancer prognosis, in addition to controlling the primary lesion. we successfully established a cell line derived from lower gingival carcinoma (WK2) as well as a line derived from secondary cervical lymph node metastasis (WK3F) through primary cultures of tissue from a patient with oral squamous cell carcinoma. We then investigated the biological characteristics of the cancer cell lines from these primary and metastatic lesions and analyzed metastasis-related genes. The results suggest that these molecules could be important for clarifying the mechanisms that regulate metastasis and provide new therapeutic targets for inhibiting tumor invasion.
I have been teaching dental students and dental residents about oral surgery.

Research

Research Interests

Biological characterization and analysis of metastasis-related genes in cell lines derived from the primary lesion and lymph node metastasis of a squamous cell carcinoma arising in the oral region
keyword : cancer cell line, metastasis-related genes
2012.04～2017.03.
The development of cancer vaccine therapy with tailor-made medicine
keyword : tumor immunity, cytotoxic T lymphocytes
2009.04～2013.03.
The tumor immunotherapy and identification of cancer-associated antigen by use of improved cDNA library method
keyword : tumor immunity, cytotoxic T lymphocytes, cancer-associated antigen
2006.09～2009.03.

Academic Activities

Books

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Papers

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1.	Takahiro Fujinaga, Kumamaru Wataru, Tsuyoshi Sugiura, Yosuke Kobayashi, YUKIKO OHYAMA, TATSUYA IKARI, Mitsuho Onimaru, Naonari Akimoto, Rumi Jogo, Yoshihide Mori, Biological characterization and analysis of metastasis-related genes in cell lines derived from the primary lesion and lymph node metastasis of a squamous cell carcinoma arising in the mandibular gingiva, International Journal of Oncology, 10.3892/ijo.2014.2332, 44, 5, 1614-1624, 2014.05, Controlling metastatic lesions is an important part of improving cancer prognosis, in addition to controlling the primary lesion. There have been numerous histological studies on primary and metastatic lesions, but little basic research has been performed using cell lines from primary and metastatic lesions belonging to the same patient. In this study, we successfully established a cell line derived from lower gingival carcinoma (WK2) as well as a line derived from secondary cervical lymph node metastasis (WK3F) through primary cultures of tissue from a patient with oral squamous cell carcinoma. We then investigated the biological characteristics of the cancer cell lines from these primary and metastatic lesions and analyzed metastasis-related genes. Comparison of the biological characteristics in vitro showed that WK3F had higher cell proliferation ability and shorter cell doubling time than WK2. WK3F also had increased cell migratory ability and higher invasive and self-replication abilities. Heterotransplantation into nude mice resulted in high tumor formation rates in the tongue and high metastasis rates in the cervical lymph nodes. Changes in WK2 and WK3F gene expression were then comprehensively analyzed using microarrays. Genes with increased expression in WK3F compared to WK2 were extracted when the Z-score was ≥2.0 and the ratio was ≥5.0, while genes with reduced expression in WK3F compared to WK2 were extracted when the Z-score was ≤ -2.0 and the ratio was ≤0.2; differences were found in 604 genes. From these, MAGEC1 (88.0 fold), MMP-7 (18.6 fold), SNAI1 (6.6 fold), MACC1 (6.2 fold), and HTRA1 (0.012 fold) were selected as metastasis-related candidate genes. The results suggest that these molecules could be important for clarifying the mechanisms that regulate metastasis and provide new therapeutic targets for inhibiting tumor invasion..
2.	Takeshi Toyoshima, Wataru Kumamaru, Jun-nosuke Hayashida, Masahumi Moriyama, Ryoji Kitamura, Hideaki Tanaka, Akira Yamada, Kyogo Itoh, Seiji Nakamura, In vitro induction of specific CD8+ T lymphocytes by tumor-associated antigenic peptides in patients with oral squamous cell carcinoma, Cancer Letters, 2012.02, The aim of this study was to clarify candidate peptides for peptide-based specific immunotherapy of patients with oral squamous cell carcinoma (SCC). Thirteen peptides were examined for in vitro induction of peptide-specific CD8+ T lymphocyte (CD8+TL) activity in peripheral blood mononuclear cells from 35patients with oral SCC. A correlation between the induction ability of CD8+TL and in vivo immune response of host was carried out immunohistochemically in 23 patients. Peptide-specific activities of CD8+TL for at least one peptide were detectable in 21/35 patients (60.0%). The potent peptides were SART-1690 in 9/35 (25.7%), SART-293, and ART475 in 7/35 (20.0%), respectively. In the 9 patients with SART-1690-specific activity, the whole of activities was significantly inducible for more number of other peptides compared to that in 26 patients without the activity (P = 0.035). Cellular responses in 7 patients with SART-1690-specific activity were significantly stronger than those in 16 patients without the activity (P = 0.027). Furthermore, the number of CD3+ T cells around the SCC was also significantly different between the 2 groups of patients (P = 0.041). In conclusion, SART-1690, SART-293, and ART475 could be applicable as peptide-based specific immunotherapies for the majority of patients with oral SCC..
3.	Wataru KUMAMARU, Seiji NAKAMURA, Tsutomu KADENA, Akira YAMADA, Eiji KAWAMURA, Masanori SASAKI, Yukiko OHYAMA, Takeshi TOYOSHIMA, Jun-nosuke HAYASHIDA, Kyogo ITOH and Kanemitsu SHIRASUNA, T-cell receptor Vbeta gene usage by T cells reactive with the tumor-rejection antigen SART-1 in oral squamous cell carcinoma, International Journal of Cancer, 108巻 5号 686～695頁 , 2004.05.

Membership in Academic Society

Japan Society for Oral Tumors
Japanese Stomatological Society
Asian Journal of Oral and Maxillofacial Surgery
Japanese Society of Oral and Maxillofacial Surgeons
Japanese　Society for Immunology

Educational

Educational Activities

I am teaching dental students and residents who came to training in oral surgery.
I am in charge of student lectures, clinical preparatory exercises and OSCE.

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