Reports

Papers

1.

Haruyoshi Yamaza, Kou Matsuo, Ieyoshi Kobayashi, Hiroko Wada, Tamotsu Kiyoshima, Merina Akhtar, Yukiko Ishibashi, Takako Sakai, Akifumi Akamine, Hidetaka Sakai, Expression of Set-α during morphogenesis of mouse lower first molar, Histochemical Journal, 10.1023/A:1014491111628, 33, 8, 437-441, 2001.12, The detailed in situ expression pattern of the Set-α gene has been studied. Previously we showed that Set-α is a differentially expressed gene in the embryonic mouse mandible at day 10.5 (E10.5) gestational age. Cells expressing Set-α were widely distributed in both the epithelial and underlying ectomesenchymal cells at E10.5. At E12, they were slightly aggregated in an area where tooth germ of the lower first molar is estimated to be formed. At E13.5, Set-α was strongly expressed in the tooth germ. At the cap stage, Set-α was expressed in the enamel organ and dental papilla. At the bell stage, Set-α was distinctly expressed in the inner enamel epithelial and dental papilla cells facing the inner enamel epithelial layer, which were intended to differentiate into ameloblasts and odontoblasts, respectively. Interestingly, Set-α was also expressed in several embryonic craniofacial tissues derived from the ectoderm. This study is the first report that Set-α is distinctly expressed in the developing tooth germ, and suggests that Set-α plays an important role in both the initiation and the growth of the tooth germ, as well as in the differentiation of ameloblasts and odontoblasts..

2.

Haruyoshi Yamaza, K. Matsuo, Tamotsu Kiyoshima, Noriatsu Shigemura, I. Kobayashi, Hiroko Wada, A. Akamime, H. Sakai, Detection of differentially expressed genes in the early developmental stage of the mouse mandible, International Journal of Developmental Biology, 45, 4, 675-680, 2001.08, We previously examined the development of the mouse mandible, and demonstrated that odontogenesis occurs between embryonic day 10.5 (E10.5) and E12. Based on the histological findings, we performed cDNA subtraction between the E10.5 and E12 mandibles to detect any differentially expressed genes which might be involved in the initiation of odontogenesis. By sequencing, homology search and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), we thus found Pgk-1, Ccte, Hsp86, Nucleolin, Hsc73, Frg1, N-ras, Set alpha and Hsj2 from the E10.5 mandible, and E25, ATPase6, Mum2, Thymosin beta4 and L21 from the E12 mandible to be differentially expressed genes. These genes are functionally related to protein transport, signal transduction, transcription, translation and molecular chaperon activity. In situ hybridization analyses of Set alpha and E25 showed that Set alpha was detected in the tooth germ at E12 and E14.5, thus indicating a close relationship of this gene to odontogenesis. Meanwhile, the in situ signal of E25 was found in the muscular layer of the tongue, thus suggesting E25 to be related to the differentiation of muscular tissue. In conclusion, we found 15 differentially expressed genes in the course of the early developmental stage of the mouse mandible using a combination of the cDNA subtraction and semi-quantitative RT-PCR methods, while in addition, two genes were demonstrated to be related to the initiation and the development of both tooth germ and the tongue according to the in situ hybridization technique..

Presentations

1.	Haruyoshi Yamaza, Bilirubin reversibly affects cell death and odontogenic capacity of SHED, ASCB|EMBO 2018 meeting, 2018.12.
2.	Haruyoshi Yamaza, Dihydroorotate dehydrogenase depletion hampersmitochondrial function and osteogenic differentiation in osteoblasts, 頭脳循環Kick Off Symposium, 2015.02.
3.	Haruyoshi Yamaza, Inhibition of mitochondrial function by depletion of Dihydroorotate dehydrogenase depletion in osteoblast, The 25th Fukuoka International Symposium On Pediatiric/Maternal-Child Health Research, 2014.08.
4.	Haruyoshi Yamaza, Kazuaki Nonaka, Dihydroorotate dehydrogenase depletion inhibits mitochondrial function in osteoblasts, Gordon Research Conferences: Craniofacial Morphogenesis & Tissue Regeneration, 2014.03.
5.	Haruyoshi Yamaza, SHED in regenerative dental medicine, USJI Week Event5: Contribution of US-Japan exchange of researchers in development of the molecular basis of dental and maxillofacial regenerative medicine leading collaboration among South-East Asian countries and US-Japan, 2013.09.
6.	Haruyoshi Yamaza, Caloric Restriction in Health, Kyudai Oral Bioscience 2013 -7th International Symposium-, 2013.03.
7.	Haruyoshi Yamaza, Stem cell researches in regenerative dentistry and the future., USJI Week Event4: Craniofacial Translational Research Based on Molecular Craniofacial Developmental Research, 2012.09.
8.	Haruyoshi Yamaza, Caloric restriction in health science, The 22th Fukuoka International Symposium On Pediatiric/Maternal-Child Health Research, 2011.09.

Pre-clinical training for Pediatric dentistry
Clinical training for Pediatric dentistry
Lecture of Pediatric dentistry
Lecture for Special needs dentistry

Oral health examination at Showa-Gakuenn in Fukutsu City, Japan
Oral health examination at Eucaly-Gakuenn, Institute for the handicapped, in Kurume City, Japan
Oral health examination at Center for the handicapped in Fukuoka City, Japan
Oral health examination at public health center of Hakata-ku and Minami-ku in Fukuka City, Japan
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