Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Akiko Maruyama-Nakashita Last modified date:2021.06.16

Associate Professor / Bioscience & Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences / Department of Bioscience and Biotechnology / Faculty of Agriculture


Presentations
1. Liu Zhang, Ryota Kawaguchi, Tomomi Morikawa-Ichinose, Alaa Allahham, Sun-Ju Kim, Akiko Maruyama-Nakashita, Glucosinolate Catabolism Dependent on Two ß-Glucosidases, BGLU28 and BGLU30, is Critical for Plant Growth under Sulfur Deficiency, 第62回日本植物生理学会年会, 2021.03.
2. NGUYEN Ha Trang, SUYAMA Akiko, OHTAKI Chihiro, MARUYAMA-NAKASHITA Akiko , Potential use of sulfur-responsive region of sulfate transporter SULTR2;1 for control of recombinant gene expression in plants, JSBBA West 3rd Student Forum, 2020.12.
3. Zhang Liu, Kawaguchi Ryota, Ichinose Tomomi, Allahham Alaa, Kim Sun-Ju, Maruyama-Nakashita Akiko, Glucosinolate catabolism dependent on two ß-Glucosidases, BGLU28 and BGLU30, is an indispensable plant survival strategy under sulfur deficiency, 日本土壌肥料学会2020 年度大会, 2020.09.
4. 張 柳、川口 諒太、一瀬 智美、Alaa Allahham、金 善州、丸山 明子, Glucosinolate catabolism dependent on two atypical myrosinases is required for sustaining plant growth under sulfur deficiency, 日本農芸化学会2020年度大会, 2020.03.
5. Alaa Allahham、丸山 明子, Sulfur deficiency induced phosphate uptake, translocation, and accumulation in Arabidopsis thaliana, 日本農芸化学会2020年度大会, 2020.03.
6. 張 柳、川口 諒太、一瀬 智美、Alaa Allahham、金 善州、丸山 明子, Two beta-Glucosidases, BGLU28 and BGLU30, are Responsible for Sulfur Deficiency-Induced Glucosinolate Catabolism in Arabidopsis, 第61回 日本植物生理学会, 2020.03.
7. Alaa Allahham, Akiko Maruyama-Nakashita, Effects of Sulfur deficiency on Phosphate accumulation, Uptake, and Translocation in Arabidopsis thaliana, 日本土壌肥料学会2019 年度大会, 2019.09.
8. ZHANG L, TAKAMUNE M, ICHINOSE T, MARUYAMA-NAKASHITA A, Involvement of Salicylic Acid to Low-Sulfur Responses in Arabidopsis thaliana, JSBBA WEST 1st Student Forum, 2018.11.
9. USHIWATARI T, MITSUDA N, MARUYAMA-NAKASHITA A, Identification of a Transcription Factor, SPL7, Maintains the Expression of SULTR2;1 in Arabidopsis Roots, JSBBA WEST 1st Student Forum, 2018.11.
10. Alaa Allahham, Akiko Maruyama-Nakashita, Involvement of Increased Xylem Transport of Phosphate in the Sulfur Deficiency- Induced Phosphate Accumulation in the Shoots of Arabidopsis thaliana, JSBBA WEST 1st Student Forum, 2018.11.
11. Alaa Allahham, Akiko Maruyama-Nakashita, Sulfur Deficiency-Induced Phosphate Accumulation in the Shoot of Arabidopsis thaliana, AFELiSA 2018, 2018.11.
12. Tomomi Ichinose, Yuzo Yamazaki, Daisuke Miura, Akiko Maruyama-Nakashita, Regulation of glucosinolate metabolism by the light conditions in Arabidopsis leaf, 11th International Plant Sulfur Workshop, 2018.09.
13. Khamsalath Soudthedlath, Akiko Maruyama-Nakashita, Phenotypic characterization of sultr2;1sultr3;5 double knockout in Arabidopsis thaliana, 日本土壌肥料学会2018年度神奈川大会, 2018.08.
14. Khamsalath Soudthedlath, Chisato Yamaguchi, Toshiki Nakamura, Tomomi Ichinose, Mori Yuki and Akiko Maruyama-Nakashita , Phenotypic characterization of sultr2;1sultr3;5 double knockout in Arabidopsis thaliana, 5th JASID Western Japan Research Meeting, 2018.08.
15. 一瀬智美、山崎雄三、三浦大典、丸山明子, 暗処理条件におけるグルコシノレート代謝動態解析, 第59回日本植物生理学会, 2018.03, Plants accumulate numerous primary and secondary metabolites to survive under various stress conditions. Glucosinolates (GSLs) are secondary metabolites that play important roles in plant defense against herbivores and pathogens. In Arabidopsis, 40 different types of aliphatic and indolic GSLs are synthesized. Analysis of GSL accumulation and degradation products at various tissues under various growth conditions provide better understanding of the biological significance of GSL in local responses against biotic stress. Spatiotemporal information of endogenous metabolites within tissue is indispensable for understanding of metabolic regulation and function of biological molecules in plants. Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) enables to visualize the distribution of several molecules at high-spatial resolution without any labelling. Previously, visualization of four types of GSLs was reported in Arabidopsis on the abaxial surface of the leaf. In this study, we investigated the distribution of GSLs present in the internal structure of the leaf by MALDI-MSI. For matrix application, we used sublimation with recrystallization method. By removing the surface layer, we succeeded to visualize the specific distribution of several types of aliphatic and indolic glucosinolates in spongy mesophyll layer with high spatial resolution. We confirmed that structurally different GSLs accumulate in the inner region of the leaf with their unique distribution profile. Using the integrated information of spatiotemporal metabolic behavior and quantitative determination of each metabolite enable us to detect local metabolic responses of GSLs in Arabidopsis..
16. 丸山明子, 草島美幸, 高宗万希子, 光田展隆, 木村侑希, 仲下英雄, 髙橋秀樹, 硫黄欠乏下での硫酸イオン吸収の増加に寄与するWRKY転写因子, 第59回日本植物生理学会, 2018.03.
17. Tomomi Ichinose, Yuzo Yamazaki, Daisuke Miura, Akiko Maruyama-Nakashita, Visualization of glucosinolate distribution by mass spectrometry imaging in Arabidopsis, AFELiSA 2017, 2018.11.
18. Alaa Allahham, Akiko Maruyama-Nakashita, Effects of Sulfur Deficiency on Phosphate Accumulation in Shoot of Arabidopsis thaliana
, AFELiSA 2017, 2018.11.
19. Khamsalath Soudthedlath, Chisato Yamaguchi, Toshiki Nakamura, Akiko Maruyama-Nakashita, Contribution of SULTR2;1 and SULTR3;5 to the Cd-induced Root-to-Shoot Translocation of Sulfate., AFELiSA 2017, 2018.11.
20. Akiko Maruyama-Nakashita, Coordination of sulfur metabolism responding to sulfate availability in the environments, TAIWAN-Japan Plant Biology 2017, 2018.11.
21. Tomomi Ichinose, Yuzo Yamazaki, Daisuke Miura, Akiko Maruyama-Nakashita, Spatial distribution of glucosinolates in the inner region of the Arabidopsis leaf, TAIWAN-Japan Plant Biology 2017, 2018.11.
22. Khamsalath Soudthedlath, Chisato Yamaguchi, Toshiki Nakamura, Akiko Maruyama-Nakashita, Contribution of SULTR2;1 and SULTR3;5 to the Cd-induced Root-to-Shoot Translocation of Sulfate., TAIWAN-Japan Plant Biology 2017, 2018.11.
23. Chisato Yamaguchi, Yuki Takimoto, Naoko Ohkama-Ohtsu, Akiko Hokura, Takuro Shinano, Toshiki Nakamura, Akiko Suyama, Akiko Maruyama-Nakashita, Mechanisms of cadmium-induced increases of sulfate uptake, translocation and thiol synthesis in Arabidopsis thaliana., TAIWAN-Japan Plant Biology 2017, 2018.11.
24. Khamsalath Soudthedlath, Chisato Yamaguchi, Akiko Maruyama-Nakashita, Contribution of SULTR2;1 and SULTR3;5 to the Root-to-Shoot Transport of Sulfate, 日本土壌肥料学会2017年度仙台大会, 2017.09.
25. Tomomi Ichinose, Yuzo Yamazaki, Daisuke Miura, Akiko Maruyama-Nakashita, Visualization of glucosinolate distribution by mass spectrometry imaging, ASPB2017, 2017.06, Plants produce diverse secondary metabolites to survive under various stress conditions. Spatiotemporal information of their metabolites within tissue is indispensable for understanding of physiological regulation and function of biological molecules in plants. Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) enables to visualize the distribution of several molecules at high-spatial resolution without any labelling.
In the present study, we investigated the distribution of metabolites present in the internal structure of the leaf by MALDI-MSI. Highly sensitive MSI for visualizing more global endogenous metabolites requires an optimal sample preparation procedure including matrix application and tissue processing in plant. Removing the epidermis is an essential factor to detect the metabolites within the mesophyll in leaves, since cuticles in higher plant leaves work as the barrier for direct MSI of internal metabolites.
We attempted to remove off the upper epidermis and palisade mesophyll from the surface layer of the leaf. For matrix application, we used sublimation with recrystallization method. By removing the surface layer, we succeeded to visualize the specific distribution of several types of indole and aliphatic glucosinolates in spongy mesophyll layer with high spatial resolution, which may be due to the improvement of metabolite-matrix co-crytstallization in the inner region of the leaf .
Herein, we succeeded to simultaneously visualize the distribution of a wide variety of glucosinolates and other endogenous metabolites in spongy mesophyll layer with their unique accumulation profiles. This result indicates that MALDI-MSI enabled us to detect local metabolic responses to various growth conditions. We concluded that successful MSI provides significant clues for elucidating the biosynthetic and degradation pathway of glucosinolates in Arabidopsis.
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26. Akiko Maruyama-Nakashita, Sulfur deficiency responses in plants: Regulation of sulfate transport and Trade-off of the primary and secondary sulfur metabolism in Arabidopsis
, 2016.09.
27. 丸山 明子, 草島 美幸, Tamara Gigolashvili, 小西 智一, 仲下 英雄, SDI1 inhibits aliphatic GSL biosynthesis through the interaction with MYB28, 第58回日本植物生理学会, 2017.03.
28. Akiko Maruyama-Nakashita, Detection and Quantification of Selenate and Chromate Using Sulfur-Responsive Regions of Sulfate Transporters
, The 5th SULPHYTON WORKSHOP, 2016.09.
29. Akiko Maruyama-Nakashita, Sulfur deficiency response in plants: Trade-off of the primary and secondary sulfur metabolism , The 5th SULPHYTON WORKSHOP, 2016.09.
30. Chisato Yamaguchi, Yuki Takimoto, Naoko Ohkama-Ohtsu, Akiko Hokura, Takuro Shinano, Toshiki Nakamura, Akiko Suyama, Akiko Maruyama-Nakashita, Effects of Cadmium Treatment on the Uptake and Translocation of Sulfate in Arabidopsis thaliana, The 5th SULPHYTON WORKSHOP, 2016.09.
31. Akiko Maruyama-Nakashita, Akiko Watanabe-Takahashi, Eri Inoue, Tomoyuki Yamaya, Kazuki Saito, Hideki Takahashi, Sulfur-Responsive Elements in 3’-Non-Transcribed Region of SULFATE TRANSPORTER 2;1 : Essential Role in Transcriptional Regulation, Sulfate Uptake and Translocation in Arabidopsis Roots under Sulfur-Deficient Conditions., The 27th International Conference on Arabidopsis Research, 2016.07.
32. Chisato Yamaguchi, Yuki Takimoto, Akiko Hokura, Naoko Ohkama-Ohtsu, Takuro Shinano, Akiko Suyama, Akiko Maruyama-Nakashita, Effects of Cadmium Treatment on Uptake and Translocation of Sulfate in Arabidopsis thaliana
, The 27th International Conference on Arabidopsis Research, 2016.07.
33. Akiko Maruyama-Nakashita, Yuki Takimoto, Eri Inoue, Kazuki Saito, Hideki Takahashi, Detection and Quantification of Selenate and Chromate Using Sulfur-Responsive Regions of Sulfate Transporters, 13th International Conference on the Biogeochemistry of Trace Elements, ICOBTE 2015 FUKUOKA, 2015.07.
34. Chisato Yamaguchi, Yuki Takimoto, Takuro Shinano, Akiko Hokura, Akiko Maruyama-Nakashita, Effects of Cadmium Treatment on Uptake and Translocation of Sulfate in Arabidopsis thaliana, 13th International Conference on the Biogeochemistry of Trace Elements, ICOBTE 2015 FUKUOKA, 2015.07.
35. Akiko Maruyama-Nakashita, Hiromi Makino, Akiko Suyama, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Sulfate transporters in Marchantia polymorpha: Molecular species and the responses to sulfur nutrition, Marchantia Workshop 2014, 2014.12.
36. Hiromi Makino, Yuka Maeda, Yuuki Jodoi, Akiko Suyama, Sakiko Ishida, Akiko Maruyama-Nakashita, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Functional analysis of MpEIL in relation to ethylene and sulfur nutrition response, Marchantia Workshop 2014, 2014.12.
37. 丸山 明子, Makiko Takamune, Yuki Kimura, Yumiko Nakamura, Kazuki Saito, Hideki Takahashi, A novel cis-acting element and transcription factor involved in the induction of SULTR1;2 expression and sulfate uptake activity under sulfur limitation in Arabidopsis roots, 9th International Workshop SULFUR METABOLISM IN PLANTS, 2014.04, Background:
When plants become sulfur deficient (-S), they increase sulfate uptake capacity as one of the acclimation processes. In Arabidopsis, the transcript level of SULTR1;2, a high-affinity sulfate transporter facilitates sulfate uptake from the environment, is highly induced under –S condition (1, 2). The –S response of SULTR1;2 is controlled by SLIM1 transcription factor (3). To determine the how sulfate uptake activity is induced by -S, we tried to identify the cis-acting element and transcription factors involved in the –S-response of SULTR1;2.

Methods:
SULTR1;2 promoter region was dissected by deletion and base substitution analysis in transgenic Arabidopsis using luciferase as a reporter. Luciferase activities in the roots of transgenic plants grown under sulfur sufficient (+S) and –S conditions were analyzed and the regions indispensable for the –S response were determined.
WRKY transcription factors which transcript levels were induced by –S in SLIM1-dependent manner were collected using microarray data (3). Transcript levels of SULTR1;2 and sulfate uptake activity were analyzed as described in 3.

Results:
The results of deletion analysis indicated that –S-responsive expression of SULTR1;2 requires the regions from –371 to –360 and –328 to –323. The –371/–360 region contained a WRKY binding sequence (TGAC), and the -328/-323 region contained a SEF4 motif (AAAAAT), respectively.
There were 3 WRKY transcription factors which transcript levels were induced by –S in wild-type plants but not in slim1 mutants. Then we isolated T-DNA insertion mutants of the 3 WRKYs, and analyzed SULTR1;2 expression and sulfate uptake activity under +S and –S conditions. Significant down-regulation was observed in a mutant under –S condition, suggesting the involvement of the WRKY transcription factor in –S-inducible expression of SULTR1;2 and sulfate uptake.

Conclusion
A novel cis-acting element and a WRKY transcription factor involved in –S response of SULTR1;2 was determined.

References
1) Shibagaki N, Rose A, McDermott JP, Fujiwara T, Hayashi H, Yoneyama T, Davies JP. Plant J. 29, 475-486.
2) Yoshimoto N, Takahashi H, Smith FW, Yamaya T, Saito K. Plant J. 29, 465-473.
3) Maruyama-Nakashita A, Nakamura Y, Tohge T, Saito K, Takahashi H Plant Cell, 18, 3235-3251.
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38. 丸山 明子, Eri Inoue, Akiko Watanabe-Takahashi, Kazuki Saito, Tomoyuki Yamaya, Hideki Takahashi, The role of sulfate transporter SULTR2;1 3’region in its sulfur limitation response and physiological function, International Workshop on Plant Membrane Biology, 2013.03.
39. Identification of WRKY transcription factors involved in sulfur deficiency inducible sulfate uptake activity.
40. Identification of WRKY transcription factors involved in sulfur deficiency inducible sulfate uptake activity.