| Akiko Maruyama-Nakashita | Last modified date：2022.06.16 |
Associate Professor /
Bioscience & Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences
Department of Bioscience and Biotechnology
Faculty of Agriculture
Department of Bioscience and Biotechnology
Faculty of Agriculture
Graduate School
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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/akiko-maruyama
Reseacher Profiling Tool Kyushu University Pure
Phone
092-802-4712
Fax
092-802-4712
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
Yes
Field of Specialization
Plant Science, Plant Nutrition
Total Priod of education and research career in the foreign country
00years00months
Outline Activities
I am responsible for undergraduate and graduate education in disciplines related to plant nutrition, i.e., Molecular Biology, Analytical Chemistry, and Plant Physiology, etc.
Our research focuses on the mechanisms and regulation of the acquisition, assimilation, and metabolism of sulfur, an essential plant nutrient, as well as the contribution of sulfur metabolism and sulfur-containing metabolites to the environmental stress responses (nutrient deficiencies, heavy metals, drought, disease, etc.) in plants.
I'm also contributing the society through volunteering activities such as education, public seminars, and committee member activities in the related academic organizations.
Our research focuses on the mechanisms and regulation of the acquisition, assimilation, and metabolism of sulfur, an essential plant nutrient, as well as the contribution of sulfur metabolism and sulfur-containing metabolites to the environmental stress responses (nutrient deficiencies, heavy metals, drought, disease, etc.) in plants.
I'm also contributing the society through volunteering activities such as education, public seminars, and committee member activities in the related academic organizations.
Research
Research Interests
Membership in Academic Society
- Effects of sulfur deficiency on the uptake and transport of other nutrients in plants
keyword : sulfate, phosphate, nitrate, transport, vascular tissues
2011.04～2020.03. - Regulatory mechanism of glucosinolates catabolism in plants
keyword : glucosinolates、synthesis、signal transduction
2016.04～2020.03. - Role of sulfur metabolism in plant response and adaptation to environmental stress
keyword : sulfur metabolism, environmental stress
2010.05～2027.05. - Essentiality of selenium for plants and plant tolerance to selenate
keyword : selenium, selenate, essentiality, tolerance
2007.05～2027.05. - Effects of heavy metal treatment on sulfur assimilation and metabolism, and the physiological meaning of the effects.
keyword : Cadmium, Sulfur, Transport, Sulfate
2010.05～2027.05. - Regulatory mechanism of sulfur assimilation and metabolism in plants
keyword : Sulfur, Sulfate transporter, Sulfur containing compounds, Transcriptional regulation, Signal transduction
2010.07～2023.07. - Identification and characterization of the sulfur response mutants to identify novel regulatory proteins of sulfur assimilation and metabolism
keyword : sulfur response mutants, regulatory proteins of sulfur assimilation and metabolism
2001.05～2014.05. - Transcriptional regulation of sulfate transporters in response to sulfur nutritional status
keyword : Sulfate transporter, Transcriptional control, Transcription factor, cis-acting element
2001.05～2027.05. - Molecular mechanism of sulfate uptake and translocation in plants
keyword : Sulfate, uptake, translocation
2001.04～2014.05. - Functional identification of the proteins which transcript accumulation are regulated by sulfur level
keyword : Functional identification、sulfur responsive genes
2001.05～2014.05. - Regulatory mechanism of glucosinolates synthesis in plants
keyword : glucosinolates、synthesis、signal transduction
2001.04～2014.05.
Books
| 1. | Akiko Maruyama-Nakashita, Naoko Ohkama-Ohtsu, Glutathione in plant growth, development and stress tolerance (Eds, MA Hossain, MG Mostofa, PD Vivancos, DJ Burritt, M. Fujita, LS Phan Tran), Springer, 2017.05, Sulfur Assimilation and Glutathione Metabolism in Plants.  |
Reports
Papers
| 1. | Li Hongqiao, Akiko Suyama, Namiki Mitani-Ueno, Ruediger Hell, Akiko Maruyama-Nakashita 学生：1人,学生以外：4人, A Low Level of NaCl Stimulates Plant Growth by Improving Carbon and Sulfur Assimilation in Arabidopsis thaliana, Plants, https://doi.org/10.3390/plants10102138, 10, 10, 2138, 2021.10, [URL], High-salinity stress represses plant growth by inhibiting various metabolic processes. In contrast to the well-studied mechanisms mediating tolerance to high levels of salt, the effects of low levels of salts have not been well studied. In this study, we examined the growth of Arabidopsis thaliana plants under different NaCl concentrations. Interestingly, both shoot and root biomass increased in the presence of 5 mM NaCl, whereas more than 10 mM NaCl decreased plant biomass. To clarify the biological mechanism by which a low level of NaCl stimulated plant growth, we analyzed element accumulation in plants grown under different NaCl concentrations. In addition to the Na and Cl contents, C, S, Zn, and Cu contents were increased under 5 mM NaCl in shoots; this was not observed at higher NaCl concentrations. Adverse effects of high salinity, such as decreased levels of nitrate, phosphate, sulfate, and some cations, did not occur in the presence of 5 mM NaCl. An increase in C was possibly attributed to increased photosynthesis supported by Cl, Zn, and Cu, which also increased in shoots after NaCl application. Salt stress-responsive gene expression was enhanced under 20 mM NaCl but not at lower doses. Among the S metabolites analyzed, cysteine (Cys) was increased by 5 mM NaCl, suggesting that S assimilation was promoted by this dose of NaCl. These results indicate the usefulness of NaCl for plant growth stimulation.. |
| 2. | Akiko Maruyama-Nakashita, Yohei Ishibashi, Kyotaro Yamamoto, Liu Zhang, Tomomi Morikawa-Ichinose, Sun-Ju Kim, Nobuya Hayashi 学生：2人,学生以外：5人, Oxygen plasma modulates glucosinolate levels without affecting lipid contents and composition in Brassica napus seeds, Bioscience, Biotechnology and Biochemistry, https://doi.org/10.1093/bbb/zbab157, 85, 12, 2434-2441, 2021.09, Rapeseed contains high levels of glucosinolates (GSLs), playing pivotal roles in defense against herbivores and pests. As their presence in rapeseed reduces the value of the meal for animal feeding, intensive efforts to reduce them produced low-seed GSL cultivars. However, there is no such variety suitable for the south part of Japan. Here, we tested the effects of cold oxygen plasma (oxygen CP) on seed germination and GSL and lipid content, in three rapeseed cultivars. According to the cultivars, oxygen CP slightly stimulated seed germination and modified the GSL levels; decreased GSL levels in Westar and Kizakinonatane but increased those in Nanashikibu. In contrast, it negligibly affected the lipid content and composition in the three cultivars. Thus, oxygen CP modulated seed GSL levels without affecting seed viability and lipid content. Future optimization of this technique may help optimize rapeseed GSL content without plant breeding.. |
| 3. | Y. Nakai, A. Maruyama-Nakashita, Biosynthesis of Sulfur-Containing Small Biomolecules in Plants., Int. J. Mol. Sci., 10.3390/ijms21103470, 21, 3470, 2020.05. |
| 4. | A. Allahham, S. Kanno, L. Zhang, A. Maruyama-Nakashita, Sulfur deficiency increases phosphate accumulation, uptake, and transport in Arabidopsis thaliana., Int. J. Mol. Sci., 10.3390/ijms21082971, 21, 2971, 2020.04. |
| 5. | T. Morikawa-Ichinose, D. Miura, L. Zhang, S.-J. Kim, A. Maruyama-Nakashita, Involvement of BGLU30 in glucosinolate catabolism in the Arabidopsis leaf under dark conditions., Plant and Cell Physiology, doi.org/10.1093/pcp/pcaa035, 61, 1095-1106, 2020.06. |
| 6. | Liu Zhang, Ryota Kawaguchi, Tomomi Morikawa-Ichinose, Alaa Allahham, Sun Ju Kim, Akiko Maruyama-Nakashita, Sulfur deficiency-induced glucosinolate catabolism attributed to two β-glucosidases, bglu28 and bglu30, is required for plant growth maintenance under sulfur deficiency, Plant and Cell Physiology, 10.1093/pcp/pcaa006, 61, 4, 803-813, 2020.04, Sulfur (S) is an essential element for plants, and S deficiency causes severe growth retardation. Although the catabolic process of glucosinolates (GSLs), the major S-containing metabolites specific to Brassicales including Arabidopsis, has been recognized as one of the S deficiency (S) responses in plants, the physiological function of this metabolic process is not clear. Two β-glucosidases (BGLUs), BGLU28 and BGLU30, are assumed to be responsible for this catabolic process as their transcript levels were highly upregulated byS. To clarify the physiological function of BGLU28 and BGLU30 and their roles in GSL catabolism, we analyzed the accumulation of GSLs and other S-containing compounds in the single and double mutant lines of BGLU28 and BGLU30 and in wild-type plants under different S conditions. GSL levels were highly increased, while the levels of sulfate, cysteine, glutathione and protein were decreased in the double mutant line of BGLU28 and BGLU30 (bglu28/30) underfiS. Furthermore, transcript level of Sulfate Transporter1;2, the main contributor of sulfate uptake from the environment, was increased in bglu28/30 mutants underfiS. With these metabolic and transcriptional changes, bglu28/30 mutants displayed obvious growth retardation underfiS. Overall, our results indicate that BGLU28 and BGLU30 are required for-S-induced GSL catabolism and contribute to sustained plant growth underfiS by recycling sulfate to primary S metabolism.. |
| 7. | Chisato Yamaguchi, Soudthedlath Khamsalath, Yuki Takimoto, Akiko Suyama, Yuki Mori, Naoko Ohkama-Ohtsu and Akiko Maruyama‐Nakashita, SLIM1 Transcription Factor Promotes Sulfate Uptake and Distribution to Shoot,Along with Phytochelatin Accumulation, Under Cadmium Stress in Arabidopsis thaliana, Plants, doi:10.3390/plants9020163, 9, 163, 2020.01. |
| 8. | Yuki Kimura, Tsukasa Ushiwatari, Akiko Suyama, Rumi Tominaga‐Wada, Takuji Wada and Akiko Maruyama‐Nakashita, Contribution of Root Hair Development to Sulfate Uptake in Arabidopsis, Plants, doi:10.3390/plants8040106, 8, 106, 2019.04. |
| 9. | Akiko Maruyama-Nakashita, Metabolic changes sustain the plant life in low-sulfur environments, Current Opinion in Plant Biology, http://dx.doi.org/10.1016/j.pbi.2017.06.015, 93, 144-151, 2017.11, Plants assimilate inorganic sulfate into various organic sulfur (S) compounds, which contributes to the global sulfur cycle in the environment as well as the nutritional supply of this essential element to animals. Plants, to sustain their lives, adapt the flow of their S metabolism to respond to external S status by activating S assimilation and catabolism of stored S compounds, and by repressing the synthesis of secondary S metabolites like glucosinolates. The molecular mechanism of this response has been gradually revealed, including the discovery of several regulatory proteins and enzymes involved in S deficiency responses. Recent progress in this research area and the remaining issues are reviewed here.. |
| 10. | Chisato Yamaguchi, Naoko Ohkama-Ohtsu, Takuro Shinano, Akiko Maruyama-Nakashita, Plants prioritize phytochelatin synthesis during cadmium exposure even under reduced sulfate uptake caused by the disruption of SULTR1;2., Plant Signaling & Behavior, http://dx.doi.org/10.1080/15592324.2017.1325053, 2017.05. |
| 11. | Akiko Maruyama-Nakashita, Akiko Suyama, Hideki Takahashi, 5′-non-transcribed flanking region and 5′-untranslated region play distinctive roles in sulfur deficiency induced expression of SULFATE TRANSPORTER 1;2 in Arabidopsis roots, Plant Biotechnology, DOI: 10.5511/plantbiotechnology.16.1226a, 34, 51-55, 2017.03. |
| 12. | Chisato Yamaguchi, Yuki Takimoto, Naoko Ohkama-Ohtsu, Akiko Hokura, Takuro Shinano, Toshiki Nakamura, Akiko Suyama, Akiko Maruyama-Nakashita, Effects of Cadmium Treatment on the Uptake and Translocation of Sulfate in Arabidopsis thaliana., Plant and Cell Physiology, 57, 2353-2366, 2016.11, Cadmium (Cd) is a highly toxic and non-essential element for plants, whereas phytochelatins and glutathione are lowmolecular- weight sulfur compounds that function as chelators and play important roles in detoxification. Cadmium exposure is known to induce the expression of sulfurassimilating enzymes and sulfate uptake by roots. However, the molecular mechanism underlying Cd-induced changes remains largely unknown. Accordingly, we analyzed the effects of Cd treatment on the uptake and translocation of sulfate and accumulation of thiols in Arabidopsis thaliana. Both wild type (WT) and null mutant (sel1-10 and sel1-18) plants of the sulfate transporter SULTR1;2 exhibited growth inhibition when treated with CdCl2. However, the mutant plants exhibited a lower growth rate and lower Cd accumulation. Cadmium treatment also upregulated the transcription of SULTR1;2 and sulfate uptake activity in WT plants, but not in mutant plants. In addition, the sulfate, phytochelatin and total sulfur contents were preferentially accumulated in the shoots of both WT and mutant plants treated with CdCl2, and sulfur K-edge XANES spectra suggested that sulfate was the main compound responsible for the increased sulfur content in the shoots of CdCl2-treated plants. Our results demonstrate that Cd-induced sulfate uptake depends on SULTR1;2 activity, and that CdCl2 treatment greatly shifts the distribution of sulfate to shoots, increases the sulfate concentration of xylem sap and upregulates the expression of SULTRs involved in root-toshoot sulfate transport. Therefore, we conclude that root-toshoot sulfate transport is stimulated by Cd and suggest that the uptake and translocation of sulfate in CdCl2-treated plants are enhanced by demand-driven regulatory networks.. |
| 13. | Fayezeh Aarabi, Miyuki Kusajima, Takayuki Tohge, Tomokazu Konishi, Tamara Gigolashvili, Makiko Takamune, Yoko Sasazaki, Mutsumi Watanabe, Hideo Nakashita, Alisdair R. Fernie, Kazuki Saito, Hideki Takahashi, Hans-Michael Hubberten, Rainer Hoefgen, Akiko Maruyama-Nakashita, Sulfur deficiency–induced repressor proteins optimize glucosinolate biosynthesis in plants, Science Advances, 2, e1601087, 2016.10, Glucosinolates (GSLs) in the plant order of the Brassicales are sulfur-rich secondary metabolites that harbor antipathogenic and antiherbivory plant-protective functions and have medicinal properties, such as carcinopreventive and antibiotic activities. Plants repress GSL biosynthesis upon sulfur deficiency (−S); hence, field performance and medicinal quality are impaired by inadequate sulfate supply. The molecular mechanism that links –S to GSL biosynthesis has remained understudied. We report here the identification of the –S marker genes sulfur deficiency induced 1 (SDI1) and SDI2 acting as major repressors controlling GSL biosynthesis in Arabidopsis under –S condition. SDI1 and SDI2 expression negatively correlated with GSL biosynthesis in both transcript and metabolite levels. Principal components analysis of transcriptome data indicated that SDI1 regulates aliphatic GSL biosynthesis as part of –S response. SDI1 was localized to the nucleus and interacted with MYB28, a major transcription factor that promotes aliphatic GSL biosynthesis, in both yeast and plant cells. SDI1 inhibited the transcription of aliphatic GSL biosynthetic genes by maintaining the DNA binding composition in the form of an SDI1-MYB28 complex, leading to down-regulation of GSL biosynthesis and prioritization of sulfate usage for primary metabolites under sulfur-deprived conditions.. |
| 14. | Akiko Maruyama-Nakashita, Combinatorial use of sulfur-responsive regions of sulfate transporters provides a highly sensitive plant-based system for detecting selenate and chromate in the environment, Soil Science and Plant Nutrition, 62, 2016.03. |
| 15. | Akiko Maruyama-Nakashita, Akiko Watanabe-Takahashi, Eri Inoue, Tomoyuki Yamaya, Kazuki Saito, Hideki Takahashi, Sulfur-responsive elements in the 3’-non-transcribed intergenic region are essential for the induction of Sulfate Transporter 2;1 gene expression in Arabidopsis roots under sulfur deficiency., The Plant Cell, 27, 1279-1296, 2015.04, Under sulfur deficiency (–S), plants induce expression of the sulfate transport systems in roots to increase uptake and root-to-shoot transport of sulfate. The low-affinity sulfate transporter SULTR2;1 is predominantly expressed in xylem parenchymaand pericycle cells in Arabidopsis thaliana roots under –S. The mechanisms underlying –S-inducible expression of SULTR2;1 in roots have remained unclear, despite the possible significance of SULTR2;1 for acclimation to low-sulfur conditions. In this investigation, examination of deletions and base substitutions in the 3'-intergenic region of SULTR2;1 revealed novel sulfur-responsive elements, SURE21A (5'-CAATGTATC-3') and SURE21B (5'-CTAGTAC-3'), located downstream of the SULTR2;1 3'-untranslated region. SURE21A and SURE21B effectively induced reporter gene expression from fusion constructs under –S in combination with minimal promoters or promoters not inducible by –S, suggesting their versatility in controlling transcription. T-DNA insertions near SURE21A and SURE21B abolished –S-inducible expression of SULTR2;1 in roots and reduced the uptake and root-to-shoot transport of sulfate. In addition, these mutations partially suppressed SULTR2;1 expression in shoots, without changing its –S-responsive expression. These findings indicate that SULTR2;1 contributes to the increase in uptake and internal translocation of sulfate driven by gene expression induced under the control of sulfur-responsive elements in the 3'-nontranscribed intergenic region of SULTR2;1.. |
| 16. | C. Kawashima, N. Yoshimoto, A. Maruyama-Nakashita, Y. Tsuchiya, K. Saito, H. Takahashi, T. Dalamy , Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. , The Plant J. , 57, 313 – 321, 2009.05. |
| 17. | M. Yasuda, A. Ishikawa, Y. Jikumaru, M. Seki, T. Umezawa, T. Asami, A. Maruyama-Nakashita, T. Kudo, K. Shinozaki, S. Yoshida, H. Nakashita , Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis. , The Plant Cell. , 2008.06. |
| 18. | A. Maruyama-Nakashita, Y. Nakamura, T. Tohge, K. Saito, H. Takahashi , Central transcriptional regulator of plant sulfur response and metabolism. , The Plant Cell , 18: 3235-3251., 2006.11. |
| 19. | A. Maruyama-Nakashita, Y. Nakamura, A. Watanabe-Takahashi, E. Inoue, T. Yamaya, H. Takahashi, Identification of a novel cis-acting element conferring sulfur deficiency response in Arabidopsis roots. , The Plant J. , 42: 305-314., 2005.05. |
| 20. | A. Maruyama-Nakashita, Y. Nakamura, T. Yamaya, H. Takahashi , A novel regulatory pathway of sulfate uptake in Arabidopsis roots: implication of CRE1/WOL/AHK4-mediated cytokinin-dependent regulation. , The Plant J. , 38: 779-789., 2004.05. |
| 21. | A. Maruyama-Nakashita, E. Inoue, A. Watanabe-Takahashi, T. Yamaya, H. Takahashi , Transcriptome profiling of sulfur-responsive genes in Arabidopsis reveals global effects of sulfur nutrition on multiple metabolic pathways., Plant Physiol. , 132: 597-605., 2003.05. |
| 22. | A. Maruyama, K. Ishizawa and K. Saito , ß-Cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation. , Plant Mol. Biol. , 46: 749-760., 2001.05. |
| 23. | A. Maruyama, K. Ishizawa and T. Takagi , Purification and characterization of ß-cyanoalanine synthase and cysteine synthases from potato tubers. ß-Cyanoalanine synthase and mitochondrial cysteine synthase are the same enzyme? , Plant Cell Physiol. , 41: 200-208., 2000.05. |
Presentations
- Japan Society for Bioscience, Biotechnology and Agrochemistry
- Japan Transporter Research Association: JTRA
- Japanese Society for Plant Biotechnology
- Japanese Society of Soil Science and Plant Nutrition
- The Japanese Society of Plant Physiologists
Educational
Educational Activities
I'm conducting the education related to Plant Nutrition and the related subject to graduate and undergraduate students.
Class for undergraduate: Experimental course, Plant Physiology & Biochemistry, Scientific English, Molecular Biology, Special Lecture on Advanced Topics of Agriculture.
Class for the graduate: Molecular Biosciences, Advanced Course of Plant Physiology and Biochemistry
Other Educational Activities
Class for undergraduate: Experimental course, Plant Physiology & Biochemistry, Scientific English, Molecular Biology, Special Lecture on Advanced Topics of Agriculture.
Class for the graduate: Molecular Biosciences, Advanced Course of Plant Physiology and Biochemistry
- 2012.04.
- 2009.04.
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