| 星野 友（ほしの ゆう） | データ更新日：2023.11.22 |
教授 ／
工学研究院
応用化学部門
分子生命工学コース
| 1. | Y Hoshino, S Tajima, H Nakayama, Y Okahata, RNA-aligned film prepared from an RNA/lipid complex, MACROMOLECULAR RAPID COMMUNICATIONS, 10.1002/1521-3927(20020301)23:43.0.CO;2-H, 23, 4, 253-255, 2002.03, Communication: Strong films were prepared from an RNA/lipid complex by casting from organic solutions. RNA (mainly tRNA) was extracted from yeasts, followed by a replacement of the sodium counterions of the tRNA phosphate units by cationic amphiphiles. RNA units in the RNA/lipid film could be aligned in one direction by stretching due to the presence of inter-molecular hydrogen bonds. The film showed physical strength and was biodegradable.. |
| 2. | T Kawasaki, Y Hoshino, Y Ishizu, Y Mizushiro, Y Okahata, Control of hydrolysis and condensation activities of thermolysin by ultrasound irradiation, CHEMISTRY LETTERS, 10.1246/cl.2005.1602, 34, 12, 1602-1603, 2005.12, Hydrolysis and condensation reactions of peptides catalyzed by thermolysin can be reversibly controlled by on/off ultrasound irradiation depending on its frequency region.. |
| 3. | Yu Hoshino, Takeo Urakami, Takashi Kodama, Hiroyuki Koide, Naoto Oku, Yoshio Okahata, Kenneth J. Shea, Design of Synthetic Polymer Nanoparticles that Capture and Neutralize a Toxic Peptide, SMALL, 10.1002/smll.200900186, 5, 13, 1562-1568, 2009.07, Designed polymer nanoparticles (NPs) capable of binding and neutralizing a biomacromolecular toxin are prepared. A library of copolymer NPs is synthesized from combinations of functional monomers. The binding capacity and affinity of the NPs are individually analyzed. NPs with optimized composition are capable of neutralizing the toxin even in a complex biological milieu. It is anticipated that this strategy will be a starting point for the design of synthetic alternatives to antibodies.. |
| 4. | SETO Hirokazu, MORII Takato, YONEDA Tamami, MURAKAMI Tatsuya, HOSHINO Yu, MIURA Yoshiko, Preparation of Palladium-loaded Polymer Nanoparticles with Catalytic Activity for Hydrogenation and Suzuki Coupling Reactions, Chemistry letters, 10.1246/cl.2013.301, 42, 3, 301-303, 2013.03, Palladium-loaded polymer nanoparticles with basic functional groups were prepared. When palladium ions were adsorbed on the polymer nanoparticles, the maximum loading capacity on the nanoparticles depended on the basic functional groups in the particles. The reduced palladium had a nanoscale average diameter. The palladium-loaded polymer nanoparticles exhibited catalytic activity for hydrogenation and Suzuki coupling reactions. The catalytic activity of the nanosized palladium-loaded nanoparticles was higher than that of bulk palladium.. |
| 5. | Hirokazu Seto, Chie Yamashita, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Mitsuhiro Matsuno, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Biotinylation of Silicon and Nickel Surfaces and Detection of Streptavidin as Biosensor, Langmuir, 10.1021/la401068n, 29, 30, 9457-9463, 2013.07, The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol-ene click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin-streptvidin-biotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.. |
| 6. | Yutaro Ogata, Hirokazu Seto, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine, Membranes, 10.3390/membranes3030169, 3, 3, 169-181, 2013.07, Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measurements. The interaction between lectin and the glycopolymer immobilized on glass slides was confirmed using fluorescent-labeled proteins. Glycopolymer-immobilized surfaces exhibited specific adsorption of the corresponding lectin, compared with bovine serum albumin. Lectins were continuously rejected by the glycopolymer-immobilized membranes. When the protein solution was permeated through the glycopolymer-immobilized membrane, bovine serum albumin was not adsorbed on the membrane surface. In contrast, concanavalin A and wheat germ agglutinin were rejected by membranes incorporating D-mannose or N-acetyl-D-glucosamine, respectively. The amounts of adsorbed concanavalin A and wheat germ agglutinin was increased five-and two-fold that of adsorbed bovine serum albumin, respectively. © 2013 by the authors licensee MDPI, Basel, Switzerland.. |
| 7. | Mengchen Yue, Yu Hoshino, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment, Angewandte Chemie International Edition, 10.1002/anie.201309758, 53, 10, 2654-2657, 2014.01, Hydrogel films composed of temperature-responsive microgel particles (GPs) containing amine groups work as stimuli-responsive carbon dioxide absorbent with a high capacity of approximately 1.7mmolg(-1). Although the dried films did not show significant absorption, the reversible absorption capacity dramatically increased by adding a small amount of water (1mLg(-1)). The absorption capacity was independent of the amount of added water beyond 1mLg(-1), demonstrating that the GP films can readily be used under wet conditions. The amount of CO2 absorbed by the GP films was proportional to their thickness up to 200-300m (maximum capacity of about 2Lm(-2)). Furthermore, the films consisting of GPs showed faster and greater absorption and desorption of CO2 than that of monolithic hydrogel films. These results indicated the importance of a fast stimulus response rate of the films that are composed of GPs in order to achieve long-range and fast diffusion of bicarbonate ions. Our study revealed the potential of stimuli-responsive GP films as energy-efficient absorbents to sequester CO2 from high-humidity exhaust gases.. |
| 8. | Mengchen Yue, Yu Hoshino, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment, Angewandte Chemie, 10.1002/ange.201309758, 126, 10, 2692-2695, 2014.01. |
| 9. | Yu Hoshino, Ryohei C. Ohashi, Yoshiko Miura, Rational Design of Synthetic Nanoparticles with a Large Reversible Shift of Acid Dissociation Constants: Proton Imprinting in Stimuli Responsive Nanogel Particles, Advanced Materials, 10.1002/adma.201305957, 26, 22, 3718-3723, 2014.03, Temperature-responsive nanogel particles with acids exhibiting large and reversible pK (a) shift are prepared by an ion-imprinting polymerization of stimuli responsive monomers, cross-linkers, and acidic monomers using protons as a template. The principles employed here should be broadly applicable to other stimuli-responsive materials and will be an integral step in the production of active proton transporter.. |
| 10. | Hirokazu Seto, Seiji Kamba, Takashi Kondo, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Novel Detection Technique for Particulate Matter in Air Using Metal Mesh Device Sensors, Chemistry Letters, 10.1246/cl.131040, 43, 4, 408-410, 2014.04, A novel technique for detection of particulate matter in air using metal mesh devices is proposed. A metal mesh device with square apertures was used as membrane filter and as band-pass filter. Frequency shifts in transmittance spectra of the metal mesh device collected with particulate matter had a linear relationship to the particulate matter concentrations with a high coefficient of determination. When metal mesh devices with different opening lengths were stacked, particulate matter was fractionalized by size.. |
| 11. | Hirokazu Seto, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Shigeki Nashima, Yoshinobu Ehara, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Metal Mesh Device Sensor Immobilized with a Trimethoxysilane-Containing Glycopolymer for Label-Free Detection of Proteins and Bacteria, ACS Applied Materials & Interfaces, 10.1021/am503003v, 6, 15, 13234-13241, 2014.08, Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L-1 using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.. |
| 12. | Hirokazu Seto, Tamami Yoneda, Takato Morii, Yu Hoshino, Yoshiko Miura, Tatsuya Murakami, Membrane reactor immobilized with palladium-loaded polymer nanogel for continuous-flow Suzuki coupling reaction, AIChE Journal, 10.1002/aic.14653, 61, 2, 582-589, 2014.10, A catalytic membrane reactor, which was immobilized with palladium-loaded nanogel particles (NPs), was developed for continuous-flow Suzuki coupling reaction. Palladium-loaded membranes were prepared by immobilization of NPs, adsorption of palladium ions, and reduction into palladium(0). The presence of palladium in the membrane was confirmed by the scanning electron microscopy; palladium aggregation was not observed. The catalytic activity of the membrane reactor in continuous-flow Suzuki coupling reaction was approximately double that of a comparable reactor in which palladium ions were directly adsorbed onto an aminated membrane. This was attributed to the formation of small palladium particles. The reusability in the continuous-flow system was higher than that in a batch system, and the palladium-loaded membrane reactor had high long-term stability. (c) 2014 American Institute of Chemical Engineers AIChE J, 61: 582-589, 2015. |
| 13. | Yu Hoshino, Haejoo Lee, Yoshiko Miura, Interaction between synthetic particles and biomacromolecules: fundamental study of nonspecific interaction and design of nanoparticles that recognize target molecules, Polymer Journal, 10.1038/pj.2014.33, 46, 9, 537-545, 2014.05, This article provides a brief review of the literature on the interaction between synthetic particles and biomacromolecules. Understanding the nonspecific interactions between biomacromolecules and synthetic particles is of great importance for using materials in vitro and in vivo. The interaction between functionalized polymer particles and proteins has been studied extensively to identify the main factor that governs the interaction in vitro. Recently, the composition and properties of the protein corona that forms on the surface of nanoparticles (NPs) in biofluids have been studied in the context of the function and distribution of the particles in vivo. In the meantime, NPs that recognize specific biomacromolecules have also been designed by tuning the combination and distribution of functional groups on gold NPs and dendrimers. It has also been shown that nano-gel particles that recognize target molecules can be achieved by (A) optimizing the combination and amount of functional groups in the particles and (B) molecular imprinting polymerization, in combination with (C) affinity purification. Some of these particles are capable of recognizing target molecules and neutralizing their function, even in the bloodstream of living animals, as 'plastic antibodies.'. |
| 14. | Mengchen Yue, Yu Hoshino, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Inside Back Cover: Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment (Angew. Chem. Int. Ed. 10/2014), Angewandte Chemie International Edition, 10.1002/anie.201401095, 53, 10, 2777-2777, 2014.02. |
| 15. | Mengchen Yue, Yu Hoshino, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Innenrücktitelbild: Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment (Angew. Chem. 10/2014), Angewandte Chemie, 10.1002/ange.201401095, 126, 10, 2817-2817, 2014.02. |
| 16. | Keiichi Yoshimatsu, Tomohiko Yamazaki, Yu Hoshino, Paul E. Rose, Linda F. Epstein, Les P. Miranda, Philip Tagari, John M. Beierle, Yusuke Yonamine, Kenneth J. Shea, Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag, Journal of the American Chemical Society, 10.1021/ja410817p, 136, 4, 1194-1197, 2014.01, We describe a novel epitope discovery strategy for creating an affinity agent/peptide tag pair. A synthetic polymer nanoparticle (NP) was used as the "bait" to catch an affinity peptide tag. Biotinylated peptide tag candidates of varied sequence and length were attached to an avidin platform and screened for affinity against the polymer NP. NP affinity for the avidin/peptide tag complexes was used to provide insight into factors that contribute NP/tag binding. The identified epitope sequence with an optimized length (tMel-tag) was fused to two recombinant proteins. The tagged proteins exhibited higher NP affinity than proteins without tags. The results establish that a fusion peptide tag consisting of optimized 15 amino acid residues can provide strong affinity to an abiotic polymer NP. The affinity and selectivity of NP/tMel-tag interactions were exploited for protein purification in conjunction with immobilized metal ion/His6-tag interactions to prepare highly purified recombinant proteins. This strategy makes available inexpensive, abiotic synthetic polymers as affinity agents for peptide tags and provides alternatives for important applications where more costly affinity agents are used.. |
| 17. | Adam Weisman, Yingyao Allie Chen, Yu Hoshino, Huiting Zhang, Kenneth Shea, Engineering Nanoparticle Antitoxins Utilizing Aromatic Interactions, Biomacromolecules, 10.1021/bm500666j, 15, 9, 3290-3295, 2014.09, Methicillin resistant Staphylococcus aureus (MRSA) is a highly virulent bacterium capable of inflicting severe infections. This pathogen has a long history of developing resistance to antibacterial drugs, and many phenotypes are capable of disabling the host immune response by releasing peptide and protein toxins with the capacity to lyse human polymorphonuclear neutrophils. The peptide phenol-soluble modulin alpha 3 (PSM alpha 3) has been identified as an important toxin released by the most virulent strains of MRSA. A library of polymer nonaparticles was synthesized by precipitation polymerization and screened for their ability to bind and neutralize this toxin. To generate high affinity, monomers were chosen to compliment the functional groups of PSM alpha 3. Nanopartides incorporating aromatic monomers provided a high affinity for the peptide and were effective at neutralizing its toxicity in vitro.. |
| 18. | Masahiko Nakamoto, Yu Hoshino, Yoshiko Miura, Effect of Physical Properties of Nanogel Particles on the Kinetic Constants of Multipoint Protein Recognition Process, Biomacromolecules, 10.1021/bm401536v, 15, 2, 541-547, 2014.02, We report the effect of physical properties, such as flexibility and polymer density, of nanogel particles (NPs) on the association/dissociation rates constant (k(on) and k(off)) and equilibrium constants (K-d) of multipoint protein recognition process. NPs having different flexibilities and densities at 25 degrees C were synthesized by tuning cross-linking degrees and the volume phase transition (VPT) temperature. Rate constants were quantified by analyzing time course of protein binding process on NPs monitored by a quartz crystal microbalance (QCM). Both k(on) and k(off) of swollen phase NPs increased with decreasing cross-linking degree, whereas cross- linking degree did not affect k(on) and k(off) of the collapsed phase NPs, indicating that polymer density of NPs governs k(on) and k(off). The results also suggest that the mechanical flexibility of NPs, defined as the Young's modulus, does not always have crucial roles in the multipoint molecular recognition process. On the other hand, K-d was independent of the cross-linking degree and depended only on the phase of NPs, indicating that molecular-scale flexibility, such as side-chain and segmental-mode mobility, as well as the conformation change, of polymer chains assist the formation of stable binding sites in NPs. Our results reveal the rationale for designing NPs having desired affinity and binding kinetics to target molecules.. |
| 19. | Yu Hoshino, Yuka Arata, Yusuke Yonamine, Shih-Hui Lee, Aki Yamasaki, Ryousuke Tsuhara, Katsuhiko Yano, Kenneth J Shea, Yoshiko Miura, Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials, Polymer Journal, 10.1038/pj.2014.101, 47, 2, 220-225, 2015.02, We describe the preparation and evaluation of nanogel-immobilized porous gel beads (GB) for application as a protein purification medium. Nanogel particles (NP) that bind with the Fc fragment of immunoglobulin G (IgG) were immobilized on the pore surface of macroporous hard GB containing quaternary ammonium cations on the surface via multipoint electrostatic interactions. The amount of NPs that were irreversibly immobilized in 1ml of GB slurry was determined to be similar to 30mg using fluorescent-labeled NPs. Images obtained via scanning electron microscopy established that the NPs were uniformly immobilized on the surface of the pores without blocking the macropores. The model target protein (IgG) was reversibly captured by the NP-immobilized GBs through NP-IgG interactions. NP-immobilized GBs have potential applications as novel affinity purification media for proteins, combining inexpensive and stable ligands with high-performance supports.. |
| 20. | Keiichi Yoshimatsu, Hiroyuki Koide, Yu Hoshino, Kenneth J Shea, Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin, Nature Protocols, 10.1038/nprot.2015.032, 10, 4, 595-604, 2015.03, Synthetic polymer nanoparticles (NPs) with intrinsic affinity for target biomacromolecules hold great promise in the development of novel tools for biological and biomedical research. We recently reported the design and synthesis of abiotic, synthetic polymer NPs with high intrinsic affinity for a peptide toxin melittin. The NP was selected by screening a small library of NPs (similar to 100 nm) composed of various ratios of monomers that contain functional groups complementary to the peptide melittin. The selected polymer NP, a co-polymer of acrylic acid (AAc), N-tert-butylacrylamide (TBAm), N-isopropylacrylamide (NIPAm) and N, N'-methylenebisacrylamide (BIS), effectively captures and neutralizes the toxicity of the peptide through a combination of electrostatic and hydrophobic interactions. This protocol describes a step-by-step procedure for the preparation and evaluation of synthetic polymer NPs for sequestration and neutralization of the target peptide toxin. The polymer NPs can be synthesized in a one-step polymerization reaction using commercially available reagents. The polymerization reaction for the synthesis of polymer NPs takes several hours, and the total protocol including subsequent purification and characterization by dynamic light scattering, NMR and toxicity neutralization assays takes 1-2 weeks in total.. |
| 21. | Yoke-Ming Wong, Yu Hoshino, Kumar Sudesh, Yoshiko Miura, Keiji Numata, Optimization of Poly(N-isopropylacrylamide) as an Artificial Amidase, Biomacromolecules, 10.1021/bm501671r, 16, 1, 411-421, 2015.01, Poly(N-isopropylacrylamide) microgel (NMG) has been developed by adding various functional groups to control surface charges, hydrophobicity, pK(a) and protein adsorption capacity. Here, we developed and optimized NMG anchored with three types of functional groups as a polymeric catalyst to hydrolyze amide bonds under optimized mild conditions. Various optimization strategies were evaluated for efficient hydrolysis activity on a p-nitroaniline-based substrate by using a colorimetric assay. Based on the results, we propose a mechanism to hydrolyze amide bonds and determine the theoretical average distance, using NMG bearing functional group of 1-vinylimidazole as the study model. The hydrolysis of amide bonds was inhibited by a transition-state protease inhibitor, which also confirmed the proposed reaction model for NMG. These results provide an insight into the strategies developed to functionalize hydrogels through an enzyme-mimic approach for future robust bio- and chemical conversions as well as therapeutic utilities.. |
| 22. | Lee Haejoo, Hoshino Yu, Wada Yusuke, Arata Yuka, Maruyama Atsushi, Miura Yoshiko, Minimization of Synthetic Polymer Ligands for Specific Recognition and Neutralization of a Toxic Peptide, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/jacs.5b05259, 137, 34, 10878-10881, 2015.09. |
| 23. | Hitoshi Tamagawa, Hiroyuki Kageyama, Yuya Oaki, Yu Hoshino, Yoshiko Miura, Hiroaki Imai, Inverse pH-response of Temperature-sensitive Copolymers by Combination with Porous CaCO3Framework, Chemistry Letters, 10.1246/cl.150676, 44, 10, 1425-1427, 2015.10, The pH response of lower critical solution temperature (LCST) of N-isopropylacrylamide (NIPAAm)/N-[3-(dimethylamino)propyl] methacrylamide (DMAPM) copolymers was switched by combination with porous CaCO3 microbeads. Although LCST of the simple copolymer hydrogels increases with decreasing pH, the critical value of the copolymers trapped in the porous framework decreases with the pH drop. The inversion of the pH response is ascribed to change of the effect of DMAPM by the presence of an ionic inorganic surface.. |
| 24. | Yoshiko Miura, Tomohiro Fukuda, Hirokazu Seto, Yu Hoshino, Development of glycosaminoglycan mimetics using glycopolymers, Polymer Journal, 10.1038/pj.2015.110, 48, 3, 229-237, 2015.11, Glycosaminoglycans (GAGs) are polysaccharides found in living systems that have key biological roles and function as polyelectrolytes owing to their large number of sulfate groups. There have been many reports describing the syntheses of GAGs and the development of GAG mimetics and analogs. The preparation of such GAG mimics has utilized versatile methods ranging from total syntheses to synthetic polymer chemistry approaches. The core of GAG mimetic production is the fusion of complex chemical structures with polymeric properties. Multivalent interactions of the saccharides with specific biological targets, such as proteins, are an essential function of GAGs and other multivalent saccharides. In this review, methods for generating GAGs from glycopolymers are presented and research reports describing the functional characterization of the synthesized GAGs are outlined.. |
| 25. | Masanori Nagao, Yuuki Kurebayashi, Hirokazu Seto, Tomonari Tanaka, Tadanobu Takahashi, Takashi Suzuki, Yu Hoshino, Yoshiko Miura, Synthesis of well-controlled glycopolymers bearing oligosaccharides and their interactions with influenza viruses, Polymer Journal, 10.1038/pj.2016.14, 48, 6, 745-749, 2016.06. |
| 26. | Hirokazu Seto, Kenta Imai, Yu Hoshino, Yoshiko Miura, Polymer microgel particles as basic catalysts for Knoevenagel condensation in water, Polymer Journal, 10.1038/pj.2016.44, 48, 8, 897-904, 2016.08, The catalytic activities of basic and thermoresponsive microgel particles for the Knoevenagel condensation reaction in water at ambient temperature were evaluated. The gel particles (GPs) were incorporated with primary-amino, tertiary-amino, imidazole or pyridyl groups. In the Knoevenagel condensation between benzaldehyde and ethyl cyanoacetate, the GPs that had no basic group did not exhibit any catalytic activity. The GPs that had primary-amino, tertiary-amino and imidazole groups exhibited catalytic activity. The catalytic activity of the basic GPs depended on their base strength. The tertiary-aminated microgel particles had quadruple and double the activities of tertiary-aminated silica gel and trimethylamine, respectively. The higher catalytic activity may be attributable to the local enrichment effect of ethyl cyanoacetate. In terms of gel geometry, the activity of the tertiary amines in the microgel particles was higher than that in the bulk gel. The geometry of the microgel particles allowed the rapid uptake of the substrates. Because the base strength of the tertiary amines in the microgel particles decreased with increasing temperature, the catalysts and residual active methylene substrates were separated by dialysis at 80 degrees C. The recovered microgel particle catalysts were recyclable for an additional Knoevenagel condensation.. |
| 27. | Hirokazu Seto, Hikaru Matsumoto, Makoto Shibuya, Takanori Akiyoshi, Yu Hoshino, Yoshiko Miura, Poly(N-isopropylacrylamide) gel-based macroporous monolith for continuous-flow recovery of palladium(II) ions, Journal of Applied Polymer Science, 10.1002/app.44385, 134, 4, 2016.09, Macroporous monoliths, composed of thermoresponsive, tertiary-aminated, and crosslinking monomers, were prepared for continuous-flow separation of palladium(II) ions. N-Isopropylacrylamide was required to form the porous structure in the monoliths, indicating that the mechanism of porous structure formation involved polymerization-induced phase separation of the poly(N-isopropylacrylamide) gel. Tertiary-aminated monoliths showed adsorption selectivity for palladium(II) ions in hydrochloric media, compared with copper(II) ions. The maximum capacities of the monoliths with tertiary amine contents of 10, 20, 30, and 70 mol % for palladium(II) ions were 0.6, 1.1, 1.3, and 2.3 mmol/g, respectively. Darcy's permeabilities of water through the macroporous monolith were 10(-14) to 10(-13) m(2), and those were comparable to that through a commercially available membrane filter with a pore size of several micrometers. In the continuous-flow process, the macroporous monolith with tertiary amine selectively adsorbed palladium(II) ions in the coexistence of copper(II) ions with 10 times higher concentration than the palladium(II) ions. The palladium(II) ions were eluted from the macroporous monolith, and the concentration of palladium(II) ions in the eluate was up to 45 times of that in the feed solution. The average enrichment factor and total recovery percentage of palladium(II) ions were 8.7 times and 95%, respectively. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44385.. |
| 28. | Xinnan Cui, Yuki Koujima, Hirokazu Seto, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Inhibition of Bacterial Adhesion on Hydroxyapatite Model Teeth by Surface Modification with PEGMA-Phosmer Copolymers, ACS Biomaterials Science & Engineering, 10.1021/acsbiomaterials.5b00349, 2, 2, 205-212, 2016.02, Modification of the interface properties on hydroxyapatite and tooth enamel surfaces was investigated to fabricate bacterial resistance in situ. A series of copolymers containing pendants of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and ethylene glycol methacrylate phosphate (Phosmer) were polymerized by conventional free radical polymerization and changing the feed ratio of monomers. The copolymers were immobilized on hydroxyapatite and tooth enamel via the affinity of phosphate groups to hydroxyapatite to form the stable and durable polymer brushes on the surfaces. The amounts of polymer immobilized depended on the phosphate group ratio in the copolymers. Surface modification altered the interfacial properties of hydroxyapatite and inhibited bacterial adhesion. Copolymers containing 40-60% PEGMA segments showed a significant inhibitory effect on bacterial adhesion of S. epidermidis both in the presence and absence of plaque model biomacromolecules.. |
| 29. | Yoshiko Miura, Yu Hoshino, Hirokazu Seto, Glycopolymer Nanobiotechnology, Chemical Reviews, 10.1021/acs.chemrev.5b00247, 116, 4, 1673-1692, 2016.02. |
| 30. | Masahiko Nakamoto, Tadashi Nonaka, Kenneth J. Shea, Yoshiko Miura, Yu Hoshino, Design of Synthetic Polymer Nanoparticles That Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme, Journal of the American Chemical Society, 10.1021/jacs.5b12600, 138, 13, 4282-4285, 2016.04, Designed polymer hydrogel nanoparticles (NPs) capable of facilitating resolubilization and refolding of an aggregated protein, positively charged lysozyme, are prepared. NPs designed to interact strongly with denatured lysozyme and relatively weakly with native lysozyme, facilitated resolubilization and refolding of aggregated lysozyme. Such NPs could be prepared by copolymerizing optimized combinations and populations of functional monomers. The refolded lysozyme showed native conformation and enzymatic activity. Eleven grams of aggregated protein was refolded by 1 g of NPs. However, NPs having low affinity to denatured lysozyme and NPs having high affinity to both denatured and native lysozyme showed relatively low facilitation activity. Our results suggest a potential strategy for the design of artificial chaperones with high facilitating activity.. |
| 31. | Yu Hoshino, Takaaki Miyoshi, Masahiko Nakamoto, Yoshiko Miura, Wide-range pK(a) tuning of proton imprinted nanoparticles for reversible protonation of target molecules via thermal stimuli, JOURNAL OF MATERIALS CHEMISTRY B, 10.1039/c7tb02107k, 5, 46, 9204-9210, 2017.12, pK(a) tuning of Bronsted acids in synthetic nano-materials is of great importance for the design of ion exchange and bio-/molecular-separation media and polymer catalysis. It has been reported that hydrogel nanoparticles with carboxylic acids that show large and reversible pK(a) shifts in response to thermal stimuli can be prepared by copolymerization of N-isopropylacrylamide (NIPAm), acrylic acids (AAc) and N, N'-methylene bisacrylamide (BIS) via the proton imprinting polymerization process. However, the reported range of pK(a) shifts is limited to the range of 5.3 to 7.5. In this study, we report a procedure to prepare proton imprinted NPs that show pK(a) shifts in the tuned pK(a) range and demonstrate applications of the NPs. pK(a) values ranging from 4.3 to 8.7 were achieved by designing the structure of monomers containing carboxylic acids and applying the proton imprinting procedure. It was demonstrated that proton-imprinted NPs with different pK(a) values could be used for the reversible and selective protonation of target molecules which have specific pK(a) values. Our results establish the generality of the proton imprinting procedure and provide a guide for designing stable and inexpensive materials for sophisticated purification processes.. |
| 32. | Yuhei Terada, Hirokazu Seto, Yu Hoshino, Tatsuya Murakami, Shuhei Shinohara, Kaoru Tamada, Yoshiko Miura, SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties, Polymer Journal, 10.1038/pj.2016.99, 49, 2, 255-262, 2017.02, Glycopolymers consisting of mannose and acrylamide with different mannose incorporation ratios (10 and 100%) were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization, and the polymer layers were prepared on a gold substrate. The detailed polymer layer structure and molecular recognition properties were analyzed by the surface plasmon resonance (SPR) technique. The glycopolymers formed pancake-like thin layers with thicknesses of similar to 2 nm in air and were swollen in the aqueous solution. The molecular recognition against concanavalin A (ConA) was also analyzed by SPR. Binding constants between glycopolymers and ConA were large enough to suggest multivalent effects. Binding rate constants of ConA to glycopolymers were in the same order; however, the dissociation rate constant was lower in the glycopolymer with a mannose ratio of 100% because of the high local mannose density near the binding point of ConA.. |
| 33. | Yu Hoshino, Rational designing of an antidote nanoparticle decorated with abiotic polymer ligands for capturing and neutralizing target toxins, Journal of Controlled Release, 10.1016/j.jconrel.2017.10.028, 268, 335-342, 2017.12, Many of macromolecular toxins induce cell death by directly interacting with cells or induction of inflammatory cytokines. Abiotic polymer ligands (PLs) composed of functional monomers are able to bind and neutralize toxins in vivo and are of great interest for efficient antidotes. However, little has been reported about recognition and neutralization of target molecules in the bloodstream because of readily elimination from the bloodstream. Here, we report a rational design of PLs-decorated lipid nanoparticles (PL-NPs) for neutralizing a target toxin in vivo. PL that decorated on the NPs would cooperatively interacts with target biomacromolecules since the lipid molecules in NPs have a high degree of freedom. In the present study, N-isopropylacrylamide based PLs interacting with histones, major mediators of sepsis, were synthesized. Affinity between PL-NPs and histones depends on monomer composition and polymer length. The optimized PL-NP showed little affinity for plasma proteins. The PL-NPs inhibited the toxicity of histones both in vitro and in vivo, suggesting that PLs on the NPs cooperatively bound to histones and neutralized their toxicity. In addition, circulation time of optimized PL was significantly prolonged by the modification onto NPs. These results provide a platform for designing antidote nanoparticles neutralizing toxic biomacromolecules.. |
| 34. | Yu Hoshino, Monitoring Photosynthetic Activity in Microalgal Cells by Raman Spectroscopy with Deuterium Oxide as a Tracking Probe, ChemBioChem, 10.1002/cbic.201700314, 18, 20, 2063-2068, 2017.10, Microalgae offer great potential for the production of biofuel, but high photosynthetic activity is demanded for the practical realisation of microalgal biofuels. To this end, it is essential to evaluate the photosynthetic activity of single microalgal cells in a heterogeneous population. In this study, we present a method to monitor the photosynthetic activity of microalgae (in particular Euglena gracilis, a microalgal species of unicellular, photosynthetic, flagellate protists as our model organism) at single-cell resolution by Raman spectroscopy with deuterium from deuterium oxide (D2O) as a tracking probe. Specifically, we replaced H2O in culture media with D2O up to a concentration of 20% without disturbing the growth rate of E.gracilis cells and evaluated C-D bond formation as a consequence of photosynthetic reactions by Raman spectroscopy. We used the probe to monitor the kinetics of the C-D bond formation in E.gracilis cells by incubating them in D2O media under light irradiation. Furthermore, we demonstrated Raman microscopy imaging of each single E.gracilis cell to discriminate deuterated cells from normal cells. Our results hold great promise for Raman-based screening of E.gracilis and potentially other microalgae with high photosynthetic activity by using D2O as a tracking probe.. |
| 35. | Hikaru Matsumoto, Hirokazu Seto, Takanori Akiyoshi, Makoto Shibuya, Yu Hoshino, Yoshiko Miura, Macroporous Monolith with Polymer Gel Matrix as Continuous-flow Catalytic Reactor, CHEMISTRY LETTERS, 10.1246/cl.170360, 46, 8, 1065-1067, 2017.08, A macroporous monolith comprising a polymer gel matrix was developed for application in continuous-flow catalytic reactions. Nanosized Pd(0) particles were loaded in a poly(N-isopropylacrylamide)-gel-based monolith by Pd(II) adsorption and subsequent reduction. The permeability of the Pd(0)-loaded monolith was compared with a commercial membrane filter with a pore size of several micrometers. The Pd(0)-loaded monolith exhibited excellent productivity in a continuous-flow Suzuki coupling reaction. Moreover, the Pd(0)-loaded monolith showed sustained performance over a 7-day period without Pd leaching.. |
| 36. | Yu Hoshino, Macroporous Monolith with Polymer Gel Matrix as Continuous-flow Catalytic Reactor, Chemistry Letters, 10.1246/cl.170360, 2017.08. |
| 37. | Hikaru Matsumoto, Hirokazu Seto, Takanori Akiyoshi, Makoto Shibuya, Yu Hoshino, Yoshiko Miura, Macroporous Gel with a Permeable Reaction Platform for Catalytic Flow Synthesis, ACS Omega, 10.1021/acsomega.7b00909, 2, 12, 8796-8802, 2017.12. |
| 38. | Yu Hoshino, Glycoglycan Mimic by Synthetic Polymers, ACS Symposium Series, 10.1021/bk-2017-1253.ch003, 2017.01. |
| 39. | Yu Hoshino, Effects of Hydrophobic Modifications and Phase Transitions of Polyvinylamine Hydrogel Films on Reversible CO2 Capture Behavior: Comparison between Copolymer Films and Blend Films for Temperature-Responsive CO2 Absorption, Macromolecular Chemistry and Physics, 10.1002/macp.201600570, 218, 8, 2017.04, The separation of CO2 from large emission sources is essential to both mitigate the greenhouse effect, as well as generate carbon-based energy. However, energy consumption of conventional CO2 separation processes, which using aqueous amine solution as absorbent, is too large. It is has been previously reported that hydrogel films that are consisting of temperature-responsive amine-containing polymers can be energy efficient CO2 absorbent-the films can reversibly capture and release large amount of CO2 via temperature-induced phase transition of hydrogels. However, the study is limited to the films consisting of gel particles of polyacrylamides. In this study, a series of hydrogel films consisting of a mass-produced amine-containing linear polymer, polyvinyl amine (PVAm), are prepared, and the efficiencies of their reversible CO2 capture are tested. The effects of hydrophobic modifications and the temperature dependent phase transition behaviors of the films on the reversible CO2 capture efficiency are studied in detail. The function of hydrogel films containing modified PVAm (copolymers), as well as blend films of nonmodified PVAm and 100% modified PVAm, are compared for the first time. The results reveal that the reversible CO2 capture efficiency of polyamine films can be improved just by blending with temperature-responsive polymers.. |
| 40. | Masanori Nagao, Yurina Fujiwara, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, Design of Glycopolymers Carrying Sialyl Oligosaccharides for Controlling the Interaction with the Influenza Virus, Biomacromolecules, 10.1021/acs.biomac.7b01426, 18, 12, 4385-4392, 2017.12, We designed glycopolymers carrying sialyl oligosac-charides by "post-click" chemistry and evaluated the interaction with the influenza virus. The glycopolymer structures were synthesized in a well-controlled manner by reversible addition-fragmentation chain transfer polymerization and the Huisgen reaction. Acrylamide-type monomers were copolymerized to give hydrophilicity to the polymer backbones, and the hydrophilicity enabled the successful introduction of the oligosaccharides into the polymer backbones. The glycopolymers with different sugar densities and polymer lengths were designed for the interaction with hemagglutinin on the-virus surface. The synthesized glycopolymers showed the specific molecular recognition against different types of influenza viruses depending on the sugar units (6'- or 3'-sialyllactose). The sugar density and the polymer length of the glycopolymers affected the interaction with the influenza-virus. Inhibitory activity of the glycopolymer against-virus infection was demonstrated.. |
| 41. | Mengchen Yue, Kenta Imai, Yoshiko Miura, Yu Hoshino, Design and preparation of thermo-responsive vinylamine-containing micro-gel particles for reversible absorption of carbon dioxide, POLYMER JOURNAL, 10.1038/pj.2017.28, 49, 8, 601-606, 2017.08, To obtain a temperature-responsive CO2 absorbent with a high amine content, temperature-responsive gel particles (GPs) consisting of poly(N-isopropylacrylamide-co-polyvinylamine) (poly(NIPAm-co-VAm)) were designed and prepared. Stable poly(N-isopropylacrylamide) GPs with a high N-vinylformamide (NVF) content were prepared in aqueous media by optimization of the concentrations of the surfactant and monomers used in the polymerization step. GPs with a high polyvinylamine (pVAm) content up to 3.2 mmol amine per g GPs (similar to 30 mol%) were prepared as a stable aqueous solution via the complete and selective hydrolysis of pNVF in the poly(NIPAm-co-NVF) GPs in a methanol solution, then dialyzed against water. The GPs with 3.2 mmol amine per g GPs pVAm showed a volume phase transition at a temperature of similar to 65 degrees C. Conductivity experiments established that the aqueous solution of the poly(NIPAm-co-VAm) GPs reversibly absorbed CO2 in response to a small thermal stimulus (30-75 degrees C). In addition, the foaming of amine-functionalized GP solutions during CO2 bubbling was prevented by increasing the amount of crosslinking in the GPs.. |
| 42. | Yu Hoshino, Design and preparation of thermo-responsive vinylamine-containing micro-gel particles for reversible absorption of carbon dioxide, Polymer Journal, 10.1038/pj.2017.28, 2017.08. |
| 43. | Yu Hoshino, Anti-biofouling phosphorylated HEMA and PEGMA block copolymers show high affinity to hydroxyapatite, Colloids and Surfaces B: Biointerfaces, 10.1016/j.colsurfb.2017.09.038, 160, 289-296, 2017.12, Four types of phosphorylated 2-hydroxyethyl methacrylate and poly(ethylene glycol) methyl ether methacrylate (PEGMA) block copolymers were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization and post-phosphorylation. These polymers were composed of different phosphate segments and similar PEG brushes. Polymers with defined phosphate segments were investigated to determine the optimal bonding affinity to hydroxyapatite (HAp). Polymers containing short phosphate segments (as low as 23 mer) were capable of immobilizing on HAp surfaces in situ in a short coating time with considerable durability. After surface modification, the dense PEG brushes altered the interfacial properties of HAp. The protein adsorption on the polymer-grafted HAp was drastically reduced compared with the bare HAp. Furthermore, the presence of the PEG brushes on the HAp surface resulted in bacterial inhibition. The polymer with the shortest phosphate segment (23 mer) showed superior inhibition ability. (C) 2017 Elsevier B.V. All rights reserved.. |
| 44. | Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Shih-Hui Lee, Ai Okajima, Saki Ariizumi, Yudai Narita, Yusuke Yonamine, Adam C. Weisman, Yuri Nishimura, Naoto Oku, Yoshiko Miura, Kenneth J. Shea, A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF(165)), NATURE CHEMISTRY, 10.1038/NCHEM.2749, 9, 7, 715-722, 2017.07, Protein affinity reagents are widely used in basic research, diagnostics and separations and for clinical applications, the most common of which are antibodies. However, they often suffer from high cost, and difficulties in their development, production and storage. Here we show that a synthetic polymer nanoparticle (NP) can be engineered to have many of the functions of a protein affinity reagent. Polymer NPs with nM affinity to a key vascular endothelial growth factor (VEGF(165)) inhibit binding of the signalling protein to its receptor VEGFR-2, preventing receptor phosphorylation and downstream VEGF(165)-dependent endothelial cell migration and invasion into the extracellular matrix. In addition, the NPs inhibit VEGF-mediated new blood vessel formation in Matrigel plugs in vivo. Importantly, the non-toxic NPs were not found to exhibit off-target activity. These results support the assertion that synthetic polymers offer a new paradigm in the search for abiotic protein affinity reagents by providing many of the functions of their protein counterparts.. |
| 45. | Yu Hoshino, A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF165), Nature Chemistry, 10.1038/nchem.2749, 2017.07. |
| 46. | Hikaru Matsumoto, Takanori Akiyoshi, Yu Hoshino, Hirokazu Seto, Yoshiko Miura, Size-tuned hydrogel network of palladium-confining polymer particles: a highly active and durable catalyst for Suzuki coupling reactions in water at ambient temperature, Polymer Journal, 10.1038/s41428-018-0102-2, 50, 12, 1179-1186, 2018.12. |
| 47. | Takahiro Oh, Masanori Nagao, Yu Hoshino, Yoshiko Miura, Self-Assembly of a Double Hydrophilic Block Glycopolymer and the Investigation of Its Mechanism, Langmuir, 10.1021/acs.langmuir.8b01527, 34, 29, 8591-8598, 2018.07. |
| 48. | Yu Hoshino, Toshiki Jibiki, Masahiko Nakamoto, Yoshiko Miura, Reversible pKa Modulation of Carboxylic Acids in Temperature-Responsive Nanoparticles through Imprinted Electrostatic Interactions, ACS Applied Materials & Interfaces, 10.1021/acsami.8b11397, 10, 37, 31096-31105, 2018.09, The acid dissociation constants (p Ka values) of Brønsted acids at the active sites of proteins are reversibly modulated by intramolecular electrostatic interactions with neighboring ions in a reaction cycle. The resulting p Ka shift is crucial for the proteins to capture, transfer, and release target ions. On the other hand, reversible p Ka modulation through electrostatic interactions in synthetic polymer materials has seldom been realized because the interactions are strongly shielded by solvation water molecules in aqueous media. Here, we prepared hydrogel nanoparticles (NPs) bearing carboxylic acid groups whose p Ka values can be reversibly modulated by electrostatic interactions with counterions in the particles. We found that the deprotonated states of the acids were stabilized by electrostatic interactions with countercations only when the acids and cations were both imprinted in hydrophobic microdomains in the NPs during polymerization. Cationic monomers, like primary amine- and guanidium group-containing monomers, which interacted strongly with growing NPs showed greater p Ka modulation than monomers that did not interact with the NPs, such as quaternary ammonium group-containing monomers. Modulation was enhanced when the guanidium moieties were protected with hydrophobic groups during polymerization, so that the guanidium ions were imprinted in the hydrophobic microdomains; the lowest p Ka of ∼4.0 was achieved as a result. The p Ka modulation of the acids could be reversibly removed by inducing a temperature-dependent volume phase transition of the gel NPs. These design principles are applicable to other stimuli-responsive materials and integral to the development of synthetic materials that can be used to capture, transport, and separate target ions.. |
| 49. | Yu Hoshino, Intelligent Image-Activated Cell Sorting, Cell, 10.1016/j.cell.2018.08.028, 175, 1, 266-276, 2018.09, A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.. |
| 50. | Xinnan Cui, Tatsuya Murakami, Yukihiko Tamura, Kazuhiro Aoki, Yu Hoshino, Yoshiko Miura, Bacterial Inhibition and Osteoblast Adhesion on Ti Alloy Surfaces Modified by Poly(PEGMA-r-Phosmer) Coating, ACS Applied Materials & Interfaces, 10.1021/acsami.8b07757, 10, 28, 23674-23681, 2018.07, We have synthesized and m olailized PEGMAsuo-Phosmer to Ti6A14V surfaces by a simple procedure to reduce bacteria -associated infection without degrading the cell response. Adhered bacteria coverage was lessened to I% on polymer -coated surfaces when exposed to Escherichia colt, Staphylococcus epiciermidis and Streptococcus inutans. ivloreover, PEGMAso-Phosrner and hornoPhosrner coatings presented better responses to MC3T3-E1 preosteoblast cells when compared with the results for PEGMA2000Phosmer-coated and raw Ti alloy surfaces. The behavior of balancing bacterial inhibition and cell attraction of the PEGMAsoogroups, with an appropriate PEG brush length facilitating greater levels when compared with that of the raw Ti alloy surface.. |
| 51. | Masanori Nagao, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, Topological Design of Star Glycopolymers for Controlling the Interaction with the Influenza Virus, Bioconjugate Chemistry, 10.1021/acs.bioconjchem.9b00134, 30, 4, 1192-1198, 2019.04, The precise design of synthetic polymer ligands using controlled polymerization techniques provides an advantage for the field of nanoscience. We report the topological design of glyco-ligands based on synthetic polymers for targeting hemagglutinin (HA, lectin on the influenza virus). To achieve precise arrangement of the glycounits toward the sugar-binding pockets of HA, triarm star glycopolymers were synthesized. The interaction of the star glycopolymers with HA was found to depend on the length of the polymer arms and was maximized when the hydrodynamic diameter of the star glycopolymer was comparable to the distance between the sugar-binding pockets of HA. Following the formula of multivalent interaction, the number of binding sites in the interaction of the glycopolymers with HA was estimated as 1.8-2.7. Considering one HA molecule has three sugar-binding pockets, these values were reasonable. The binding mode of synthetic glycopolymer-ligands toward lectins could be tuned using controlled radical polymerization techniques.. |
| 52. | Masanori Nagao, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, Synthesis of Various Glycopolymers Bearing Sialyllactose and the Effect of Their Molecular Mobility on Interaction with the Influenza Virus, Biomacromolecules, 10.1021/acs.biomac.9b00515, 20, 7, 2763-2769, 2019.07, Synthetic glyco-ligands are promising candidates for effective nanomedicines against pathogens. Glycopolymers bearing sialyl-oligosaccharides interact with hemagglutinin present on the surface of influenza viruses. In designing new glycopolymers that further enhance the interaction with viruses, both static and dynamic properties of the glycopolymers should be considered. In this report, we evaluated the correlation between dynamic properties of glycopolymers and their interaction with the influenza virus. Glycopolymers with pendant sialyllactoses and various linker structures were synthesized, and their molecular mobility was determined by proton spin-spin relaxation time measurements. The molecular mobility of the glycounits increased as the length of the linker structures increased. Interestingly, glycopolymers with the medium-length linker structure exhibited the strongest interaction with the influenza virus, suggesting that optimal molecular mobility is required for maximizing multivalent interactions with the target.. |
| 53. | Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Saki Ariizumi, Anna Okishima, Takafumi Ide, Hiromichi Egami, Yoshitaka Hamashima, Yuri Nishimura, Hiroaki Kanazawa, Yoshiko Miura, Tomohiro Asai, Naoto Oku, Kenneth J Shea, Sequestering and inhibiting a vascular endothelial growth factor in vivo by systemic administration of a synthetic polymer nanoparticle., Journal of controlled release : official journal of the Controlled Release Society, 10.1016/j.jconrel.2018.12.033, 295, 13-20, 2019.02, Protein affinity reagents (PARs), frequently antibodies, are essential tools for basic research, diagnostics, separations and for clinical applications. However, there is growing concern about the reproducibility, quality and cost of recombinant and animal-derived antibodies. This has prompted the development of alternatives that could offer economic, and time-saving advantages without the use of living organisms. Synthetic copolymer nanoparticles (NPs), engineered with affinity for specific protein targets, are potential alternatives to PARs. Although there are now a number of examples of abiotic protein affinity reagents (APARs), most have been evaluated in vitro limiting a realistic assessment of their potential for more demanding, practical in vivo applications. We demonstrate for the first time that an abiotic copolymer hydrogel nanoparticle (NP1) engineered to bind a key signaling protein, vascular endothelial growth factor (VEGF165), functions in vivo to suppress tumor growth by regulating angiogenesis. Lightly cross-linked N-isopropylacrylamide based NPs that incorporate both sulfated N-acetylglucosamine and hydrophobic monomers were optimized by dynamic chemical evolution for VEGF165 affinity. NP1 efficacy in vivo was evaluated by systemic administration to tumor-bearing mice. The study found that NP1 suppresses tumor growth and reduces tumor vasculature density. Combination therapy with doxorubicin resulted in increased doxorubicin concentration in the tumor and dramatic inhibition of tumor growth. NP1 treatment did not show off target anti-coagulant activity. In addition, >97% of injected NPs are rapidly excreted from the body following IV injection. These results establish the use of APARs as inhibitors of protein-protein interactions in vivo and may point the way to their broader use as abiotic, cost effective protein affinity reagents for the treatment of certain cancers and more broadly for regulating signal transduction.. |
| 54. | Yuri Kimoto, Yuhei Terada, Yu Hoshino, Yoshiko Miura, Screening of a Glycopolymer Library of GM1 Mimics Containing Hydrophobic Units Using Surface Plasmon Resonance Imaging, ACS Omega, 10.1021/acsomega.9b02877, 4, 24, 20690-20696, 2019.12, Effective screening methods for the development of glycopolymers as molecular recognition materials are desirable for the discovery of novel biofunctional materials. A glycopolymer library was prepared to obtain guidelines for the design of glycopolymers for the recognition of cholera toxin B subunits (CTB). Glycopolymers with varying ratios of hydrophobic and sugar units were synthesized by reversible addition fragmentation chain transfer polymerization. N-tert-Butylacrylamide, N-phenylacrylamide, and N-cyclohexylacrylamide as hydrophobic units were copolymerized in the polymer backbone, and galactose, which contributes to CTB recognition, was introduced into the side chains by "post-click" chemistry. The thiol-terminated glycopolymers were immobilized on a gold surface. The polymer immobilization substrate was analyzed in terms of interaction with galactose recognition proteins (CTB, peanut agglutinin, and Ricinus communis agglutinin I) using surface plasmon resonance imaging. The polymers with high ratios of sugar and hydrophobic units had the strongest interactions with the CTB, which was different from the trend with peanut agglutinin and Ricinus communis agglutinin I. The binding constant of the CTB with the glycopolymer with hydrophobic units was 4.1 × 106 M-1, which was approximately eight times larger than that of the polymer without hydrophobic units. A correlation was observed between the log P value and the binding constant, indicating that the hydrophobic interaction played an important role in binding. New guidelines for the design of recognition materials were obtained by our screening method.. |
| 55. | Masanori Nagao, Yu Hoshino, Yoshiko Miura, Quantitative preparation of multiblock glycopolymers bearing glycounits at the terminal segments by aqueous reversible addition-fragmentation chain transfer polymerization of acrylamide monomers, Journal of Polymer Science Part A: Polymer Chemistry, 10.1002/pola.29344, 2019.04. |
| 56. | Takahiro Oh, Kazuki Jono, Yuri Kimoto, Yu Hoshino, Yoshiko Miura, Preparation of multifunctional glycopolymers using double orthogonal reactions and the effect of electrostatic groups on the glycopolymer–lectin interaction, Polymer Journal, 10.1038/s41428-019-0244-x, 51, 12, 1299-1308, 2019.12. |
| 57. | Nobutoshi Ota, Yusuke Yonamine, Takuya Asai, Yaxiaer Yalikun, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Yo Tanaka, Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis, Analytical Chemistry, 10.1021/acs.analchem.9b01007, 91, 15, 9631-9639, 2019.08, Time-course analysis of single cells is important to characterize heterogeneous activities of individual cells such as the metabolic response to their environment. Single-cell isolation is an essential step prior to time-course analysis of individual cells by collecting, culturing, and identifying multiple single-cell targets. Although single-cell isolation has been performed by various methods previously, a glass microfluidic device with semiclosed microchannels dramatically improved this process with its simple operation and easy transfer for time-course analysis of identified single cells. This study demonstrates isolating single cells of the highly motile microalgae, Euglena gracilis, by semiclosed microchannels with liquid flow only. The isolated single cells were identified in isolating channels and continuously cultured to track, by Raman microscopy, for the formation of subcellular granules composed of polysaccharide paramylon, a unique metabolite of E. gracilis, generated through photosynthesis. Through low-temperature glass bonding, a thin glass interface was incorporated to the microfluidic device. Thus, the device could perform the direct measurements of cultured single cells at high magnification by Raman microscopy with low background noise. In this study, the first demonstration of sequential monitoring of paramylon biogenesis in a single identified E. gracilis cell is shown.. |
| 58. | Yu Hoshino, High-throughput label-free molecular fingerprinting flow cytometry, Science Advances, 10.1126/sciadv.aau0241, 5, 1, eaau0241, 2019.01, Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm−1) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of |
| 59. | Yuhei Terada, Yu Hoshino, Yoshiko Miura, Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition, Chemistry – An Asian Journal, 10.1002/asia.201900053, 14, 7, 1021-1027, 2019.04, Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.. |
| 60. | Hiroyuki Koide, Tatsuya Fukuta, Anna Okishim, Saki Ariizumi, Chiaki Kiyokawa, Hiroki Tsuchida, Masahiko Nakamoto, Keiichi Yoshimatsu, Hidenori Ando, Takehisa Dewa, Tomohiro Asai, Naoto Oku, Yu Hoshino, Kenneth J Shea, Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery., Biomacromolecules, 10.1021/acs.biomac.9b00611, 20, 10, 3648-3657, 2019.10, The affinity of a synthetic polymer nanoparticle (NP) to a target biomacromolecule is determined by the association and dissociation rate constants (kon, koff) of the interaction. The individual rates and their sensitivity to local environmental influences are important factors for the on-demand capture and release a target biomacromolecule. Positively charged NPs for small interfering RNA (siRNA) delivery is a case in point. The knockdown efficacy of siRNA can be strongly influenced by the binding kinetics to the NP. Here, we show that kon and koff of siRNA to NPs can be individually engineered by tuning the chemical structure and composition of the NP. N-Isopropylacrylamide-based NPs functionalized with hydrophobic and amine monomers were used. koff decreased by increasing the amount of amine groups in the NP, whereas kon did not change. Importantly, NPs showing a low koff at pH 5.5 together with a high koff at pH 7.4 showed high knockdown efficiency when NP/siRNA complexes were packaged in lipid nanoparticles. These results provide direct evidence for the premise that the efficacy of an siRNA delivery vector is linked with the strong affinity to the siRNA in the endosome and low affinity in the cytoplasm.. |
| 61. | Anna Okishima, Hiroyuki Koide, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of Synthetic Polymer Nanoparticles Specifically Capturing Indole, a Small Toxic Molecule, Biomacromolecules, 10.1021/acs.biomac.8b01820, 20, 4, 1644-1654, 2019.04, Synthetic polymers are of interest as stable and cost-effective biomolecule-affinity reagents, since these polymers interact with target biomolecules both in vitro and in the bloodstream. However, little has been reported about orally administered polymers capable of capturing a target molecule and inhibiting its intestinal absorption. Here, we describe the design of synthetic polymer nanoparticles (NPs) specifically capturing indole, a major factor exacerbating chronic kidney disease, in the intestine. N-isopropylacrylamide-based NPs were prepared with various hydrophobic monomers. The amounts of indole captured by NPs depended on the structures and feed ratios of the hydrophobic monomers and the polymer density but not on the particle size. The combination of hydrophobic and quadrupole interaction was effective to enhance the affinity and specificity of NPs for indole. The optimized NPs specifically inhibited intestinal absorption of orally administered indole in mice. These results showed the potential of synthetic polymer NPs for inhibiting the intestinal absorption of a target molecule.. |
| 62. | Yuhei Terada, Yu Hoshino, Yoshiko Miura, Cover Feature: Glycopolymers Mimicking GM1 Gangliosides: Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition (Chem. Asian J. 7/2019), Chemistry – An Asian Journal, 10.1002/asia.201900255, 14, 7, 918-918, 2019.04. |
| 63. | Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda, Author Correction: A practical guide to intelligent image-activated cell sorting., Nature protocols, 10.1038/s41596-019-0252-5, 14, 11, 3273-3273, 2019.11, An amendment to this paper has been published and can be accessed via a link at the top of the paper.. |
| 64. | J. Gao, Yida Liu, Yu Hoshino, Gen Inoue, Amine-containing nanogel particles supported on porous carriers for enhanced carbon dioxide capture, Applied Energy, 10.1016/j.apenergy.2019.113567, 253, 2019.11, © 2019 Elsevier Ltd Amine-containing nanogel particles with a lower cooling and heating cycles (303–348 K), which is beneficial to limit the degradation and volatile of amine, has been developed as promising absorbents for CO2 capture. It is extremely important to understand the CO2 capture kinetics and mechanisms for design and operation of corresponding processes. In present work, poly tetra fluoroethylene and carbon paper were used to support the GPs by filtering and loading method. The CO2 uptake and release performance of poly tetra fluoroethylene and carbon paper supported nanogel particles was measured. It was found that carbon paper as supporter shows higher CO2 capacity and desorption rate. A comparison of three different kinetic models shows that carbon paper as supporter gives a more enhanced kinetic behavior. Avrami's fractional order model presents the best fit to experimental data. To further investigate the mechanism of CO2 uptake on absorbents, interparticle diffusion model, intraparticle diffusion model and Boyd's film diffusion model were also applied. Film diffusion resistance governed the mass transfer rate of CO2 uptake on studied absorbents at the initial CO2 uptake stages. In the following stages, intra-particle diffusion resistance plays a major role until the equilibrium is reached. The sensible heat of carbon paper as supporter is about 25% lower than that of poly tetra fluoroethylene as supporter. The desorption activation energy obtained from the Arrhenius equation is 18 kJ/mol for carbon paper as supporter, which is 84% lower than that of the typical aqueous MEA solvent.. |
| 65. | Yu Hoshino, A practical guide to intelligent image-activated cell sorting, Nature Protocols, 10.1038/s41596-019-0183-1, 14, 8, 2370-2415, 2019.07, Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capabilities of fluorescence-activated cell sorting (FACS) from fluorescence intensity profiles of cells to multidimensional images, thereby enabling high-content sorting of cells or cell clusters with unique spatial chemical and morphological traits. Therefore, iIACS serves as an integral part of holistic single-cell analysis by enabling direct links between population-level analysis (flow cytometry), cell-level analysis (microscopy), and gene-level analysis (sequencing). Specifically, iIACS is based on a seamless integration of high-throughput cell microscopy (e.g., multicolor fluorescence imaging, bright-field imaging), cell focusing, cell sorting, and deep learning on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, intelligent decision making, and actuation. Here, we provide a practical guide to iIACS that describes how to design, build, characterize, and use an iIACS machine. The guide includes the consideration of several important design parameters, such as throughput, sensitivity, dynamic range, image quality, sort purity, and sort yield; the development and integration of optical, microfluidic, electrical, computational, and mechanical components; and the characterization and practical usage of the integrated system. Assuming that all components are readily available, a team of several researchers experienced in optics, electronics, digital signal processing, microfluidics, mechatronics, and flow cytometry can complete this protocol in ~3 months.. |
| 66. | Ryutaro Honda, Akira Hamasaki, Yoshiko Miura, Yu Hoshino, Thermoresponsive CO2 absorbent for various CO2 concentrations: tuning the pKa of ammonium ions for effective carbon capture, Polymer Journal, 10.1038/s41428-020-00407-5, 53, 1, 157-167, 2020.09. |
| 67. | Benshuai Guo, Yu Hoshino, Fan Gao, Keisuke Hayashi, Yoshiko Miura, Nobuo Kimizuka, Teppei Yamada, Thermocells Driven by Phase Transition of Hydrogel Nanoparticles, Journal of the American Chemical Society, 10.1021/jacs.0c08600, 142, 41, 17318-17322, 2020.10, Thermoelectric conversion of low temperature, delocalized, and abundant thermal sources is crucial for the development of the Internet of Things (IoT) and/or a carbon-free society. Thermocells are of great interest in thermoelectric conversion of low-temperature heat due to the low cost and flexibility of components. However, significant improvement of the conversion efficiency is required for the practical use of the cells. Here, we report thermo-electrochemical cells driven by volume phase transition (VPT) of hydrogel nanoparticles (NPs). Entropically driven VPT of poly(N-isopropylacrylamide) NPs containing carboxylic acids and amines generates a pH gradient of up to 0.049 and -0.053 pH K-1, respectively, around physiological temperature. The pH gradient triggers the proton-coupled electron transfer (PCET) reactions of quinhydrone on the electrodes, resulting in the highly efficient thermoelectric conversion with a Seebeck coefficient (Se) of -6.7 and +6.1 mV K-1. Thermocells driven by phase transition of hydrogels provide a nontoxic, flexible, and inexpensive charger that harvests carbon-free energy from abundant energy sources such as solar, body and waste heat.. |
| 68. | Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda, Raman image-activated cell sorting., Nature communications, 10.1038/s41467-020-17285-3, 11, 1, 3452-3452, 2020.07, The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.. |
| 69. | Hikaru Matsumoto, Yu Hoshino, Tomohiro Iwai, Masaya Sawamura, Yoshiko Miura, Polystyrene-Supported PPh(3)in Monolithic Porous Material: Effect of Cross-Linking Degree on Coordination Mode and Catalytic Activity in Pd-Catalyzed C-C Cross-Coupling of Aryl Chlorides, CHEMCATCHEM, 10.1002/cctc.202000651, 12, 16, 4034-4037, 2020.08, Hybridization of porous synthetic polymer and sophisticated ligands play an important role in transition-metal catalysis for chemical transformations at laboratory and industrial levels. A monolithic porous polymer, which is a single piece with continuous macropores, is desired for high permeability, fast mass transfer properties, high stability, and easy modification. Herein, we first develop a monolithic porous polystyrene containing three-fold cross-linked PPh3(M-PS-TPP) for transition-metal catalysis. The monolithic and macroporous structure ofM-PS-TPPwas fabricated via polymerization-induced phase separation using porogenic solvent. Moreover, theM-PS-TPPwas synthesized using different feed ratios of divinylbenzene (DVB) for site-isolation and mono-P-ligating behavior of PPh3.P-31 CP/MAS NMR analysis revealed that the different selectivity ofM-PS-TPPs was obtained in formation of mono-P-ligation toward Pd-II. The macroporous properties and controlled mono-P-ligating behavior ofM-PS-TPPfacilitated the challenging Pd-catalyzed Suzuki-Miyaura cross-coupling reaction of chloroarenes.. |
| 70. | Hikaru Matsumoto, Yu Hoshino, Tomohiro Iwai, Masaya Sawamura, Yoshiko Miura, Polystyrene-Cross-Linking Triphenylphosphine on a Porous Monolith: Enhanced Catalytic Activity for Aryl Chloride Cross-Coupling in Biphasic Flow, Industrial & Engineering Chemistry Research, 10.1021/acs.iecr.0c02404, 59, 34, 15179-15187, 2020.08, Immobilized transition metals for continuous-flow catalyses are greatly in demand to achieve automation, scale-up, facile separation, regeneration, and energy-saving production with high level of sustainability and efficiency. Here, we report a tertiary phosphine immobilized on a macroporous monolith (M-PS-TPP) for the challenging Pd-catalyzed cross-coupling reaction of aryl chloride in a continuous-flow system. The monolithic and macroporous structure of M-PS-TPP was fabricated by bulk polymerization in the presence of a high internal phase emulsion (HIPE) template. Owing to the large pore size and high porosity, the M-PS-TPP showed high permeability against continuous flow of the mobile phase. The continuous-flow Suzuki-Miyaura cross-coupling reaction was realized by permeation of organic/aqueous media containing inorganic salt through a Pd-loaded monolith (M-PS-TPP-Pd) column without serious clogging. Controlling coordination chemistry and hydrodynamics of M-PS-TPP-Pd boosted highly active phosphine-metal complex formation and fast mass transfer of reactants. Indeed, the M-PS-TPP-Pd column showed surprisingly higher yields (similar to 93%) and turnover numbers (2704) under continuous-flow conditions than that under batch conditions (similar to 6%).. |
| 71. | Jubao Gao, Yida Liu, Yuki Terayama, Kota Katafuchi, Yu Hoshino, Gen Inoue, Polyamine nanogel particles spray-coated on carbon paper for efficient CO |
| 72. | Masaya Kichize, Masanori Nagao, Yu Hoshino, Yoshiko Miura, Multi-block and sequence-controlled polymerization of glycopolymers, and interaction with lectin, European Polymer Journal, 10.1016/j.eurpolymj.2020.110044, 140, 110044-110044, 2020.11. |
| 73. | Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Intelligent image-activated cell sorting 2.0., Lab on a chip, 10.1039/d0lc00080a, 20, 13, 2263-2273, 2020.06, The advent of intelligent image-activated cell sorting (iIACS) has enabled high-throughput intelligent image-based sorting of single live cells from heterogeneous populations. iIACS is an on-chip microfluidic technology that builds on a seamless integration of a high-throughput fluorescence microscope, cell focuser, cell sorter, and deep neural network on a hybrid software-hardware data management architecture, thereby providing the combined merits of optical microscopy, fluorescence-activated cell sorting (FACS), and deep learning. Here we report an iIACS machine that far surpasses the state-of-the-art iIACS machine in system performance in order to expand the range of applications and discoveries enabled by the technology. Specifically, it provides a high throughput of ∼2000 events per second and a high sensitivity of ∼50 molecules of equivalent soluble fluorophores (MESFs), both of which are 20 times superior to those achieved in previous reports. This is made possible by employing (i) an image-sensor-based optomechanical flow imaging method known as virtual-freezing fluorescence imaging and (ii) a real-time intelligent image processor on an 8-PC server equipped with 8 multi-core CPUs and GPUs for intelligent decision-making, in order to significantly boost the imaging performance and computational power of the iIACS machine. We characterize the iIACS machine with fluorescent particles and various cell types and show that the performance of the iIACS machine is close to its achievable design specification. Equipped with the improved capabilities, this new generation of the iIACS technology holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology.. |
| 74. | Jubao Gao, Yu Hoshino, Gen Inoue, Honeycomb-carbon-fiber-supported amine-containing nanogel particles for CO |
| 75. | Yu Hoshino, Shohei Taniguchi, Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura, Homogeneous Oligomeric Ligands Prepared via Radical Polymerization that Recognize and Neutralize a Target Peptide, Angewandte Chemie International Edition, 10.1002/anie.201910558, 59, 2, 679-683, 2020.01, Abiotic ligands that bind to specific biomolecules have attracted attention as substitutes for biomolecular ligands, such as antibodies and aptamers. Radical polymerization enables the production of robust polymeric ligands from inexpensive functional monomers. However, little has been reported about the production of monodispersed polymeric ligands. Herein, we present homogeneous ligands prepared via radical polymerization that recognize epitope sequences on a target peptide and neutralize the toxicity of the peptide. Taking advantage of controlled radical polymerization and separation, a library of multifunctional oligomers with discrete numbers of functional groups was prepared. Affinity screening revealed that the sequence specificity of the oligomer ligands strongly depended on the number of functional groups. The process reported here will become a general step for the development of abiotic ligands that recognize specific peptide sequences.. |
| 76. | Yu Hoshino, Shohei Taniguchi, Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura, Homogeneous Oligomeric Ligands Prepared via Radical Polymerization that Recognize and Neutralize a Target Peptide, Angewandte Chemie, 10.1002/ange.201910558, 132, 2, 689-693, 2020.01. |
| 77. | Yida Liu, Takashi Kodama, Taisuke Kojima, Ikuo Taniguchi, Hirokazu Seto, Yoshiko Miura, Yu Hoshino, Fine-tuning of the surface porosity of micropatterned polyethersulfone membranes prepared by phase separation micromolding, Polymer Journal, 10.1038/s41428-019-0298-9, 52, 4, 397-403, 2020.04. |
| 78. | Ryutaro Honda, Tomohiro Gyobu, Hideto Shimahara, Yoshiko Miura, Yu Hoshino, Electrostatic Interactions between Acid-/Base-Containing Polymer Nanoparticles and Proteins: Impact of Polymerization pH, ACS Applied Bio Materials, 10.1021/acsabm.0c00390, 3, 6, 3827-3834, 2020.06. |
| 79. | Yu Hoshino, Mitsunori Moribe, Naoki Gondo, Toshiki Jibiki, Masahiko Nakamoto, Benshuai Guo, Rinoka Adachi, Yoshiko Miura, Combining Acid- and Base-Imprinted Nanoparticles in a Hydrogel Film for Temperature-Responsive Quick and Reversible Capture of Salt, ACS Applied Polymer Materials, 10.1021/acsapm.9b00940, 2, 2, 505-514, 2020.02. |
| 80. | Hiroyuki Koide, Anna Okishima, Yu Hoshino, Yuri Kamon, Keiichi Yoshimatsu, Kazuhiro Saito, Ikumi Yamauchi, Saki Ariizumi, Yuqi Zhou, Ting-Hui Xiao, Keisuke Goda, Naoto Oku, Tomohiro Asai, Kenneth J Shea, Synthetic hydrogel nanoparticles for sepsis therapy., Nature communications, 10.1038/s41467-021-25847-2, 12, 1, 5552-5552, 2021.09, Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therapeutic agent for late-stage sepsis. The HNP captures and neutralizes all variants of histones, a major inflammatory mediator released during sepsis. The highly optimized HNP has high capacity and long-term circulation capability for the selective sequestration and neutralization of histones. Intravenous injection of the HNP protects mice against a lethal dose of histones through the inhibition of platelet aggregation and migration into the lungs. In vivo administration in murine sepsis model mice results in near complete survival. These results establish the potential for synthetic, nonbiological polymer hydrogel sequestrants as a new intervention strategy for sepsis therapy and adds to our understanding of the importance of histones to this condition.. |
| 81. | Benshuai Guo, Yoshiko Miura, Yu Hoshino, Rational Design of Thermocells Driven by the Volume Phase Transition of Hydrogel Nanoparticles, ACS Applied Materials & Interfaces, 10.1021/acsami.1c07266, 13, 27, 32184-32192, 2021.07. |
| 82. | Yusuke Yonamine, Takuya Asai, Yuta Suzuki, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Probing the Biogenesis of Polysaccharide Granules in Algal Cells at Sub-Organellar Resolution via Raman Microscopy with Stable Isotope Labeling., Analytical chemistry, 10.1021/acs.analchem.1c03216, 93, 50, 16796-16803, 2021.12, Phototrophs assimilate CO2 into organic compounds that accumulate in storage organelles. Elucidation of the carbon dynamics of storage organelles could enhance the production efficiency of valuable compounds and facilitate the screening of strains with high photosynthetic activity. To comprehensively elucidate the carbon dynamics of these organelles, the intraorganellar distribution of the carbon atoms that accumulate at specific time periods should be probed. In this study, the biosynthesis of polysaccharides in storage organelles was spatiotemporally probed via stimulated Raman scattering (SRS) microscopy using a stable isotope (13C) as the tracking probe. Paramylon granules (a storage organelle of β-1,3-glucan) accumulated in a unicellular photosynthetic alga, Euglena gracilis, were investigated as a model organelle. The carbon source of the culture medium was switched from NaH12CO3 to NaH13CO3 during the production of the paramylon granules; this resulted in the distribution of the 12C and 13C constituents in the granules, so that the biosynthetic process could be tracked. Taking advantage of high-resolution SRS imaging and label switching, the localization of the 12C and 13C constituents inside a single paramylon granule could be visualized in three dimensions, thus revealing the growth process of paramylon granules. We propose that this method can be used for comprehensive elucidation of the dynamic activities of storage organelles.. |
| 83. | Masanori Nagao, Masaya Kichize, Yu Hoshino, Yoshiko Miura, Influence of Monomer Structures for Polymeric Multivalent Ligands: Consideration of the Molecular Mobility of Glycopolymers, Biomacromolecules, 10.1021/acs.biomac.1c00553, 22, 7, 3119-3127, 2021.07. |
| 84. | Hiroyuki Koide, Naoki Hayashi, Go Yasuno, Anna Okishima, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of synthetic polymer nanoparticles that inhibit glucose absorption from the intestine, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2021.05.005, 561, 1-6, 2021.07. |
| 85. | Hiroyuki Koide, Naoki Hayashi, Go Yasuno, Anna Okishima, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of synthetic polymer nanoparticles that inhibit glucose absorption from the intestine., Biochemical and biophysical research communications, 10.1016/j.bbrc.2021.05.005, 561, 1-6, 2021.07, Synthetic polymers prepared using several functional monomers have attracted attention as cost-effective protein affinity reagents and alternative to antibodies. We previously reported the synthesis of poly NIPAm-based nanoparticles (NPs) using several functional monomers that can capture target molecules. In this study, we designed NPs for capturing glucose and inhibiting intestinal absorption in living mice. For capturing glucose, we focused on the Maillard reaction between primary amines and aldehyde residues. We hypothesized that the primary amine-containing NPs can capture the open-chain structure of glucose via the Maillard reaction and inhibit intestinal absorption. NPs were prepared by the precipitation polymerization of NIPAm, N-tert-butylacrylamide (TBAm), trifluoroacetate-protected N-(3-aminopropyl)methacrylamide (T-APM), and N,N'-methylenebisacrylamide. Then, T-APM in NPs was deprotected by NH3 (aq). The amount of glucose captured by NPs depended on the percentage of TBAm and APM in vitro. After 24 h, only 2% of orally administered NPs remained in the body after administration, suggesting that many NPs were excreted without being absorbed. The prepared NPs significantly inhibited an increase in blood glucose concentration after the oral administration of glucose and NPs, indicating that NPs capture glucose and inhibit intestinal absorption. These results show the potential of using synthetic polymer nanoparticles for inhibiting postprandial hyperglycemia.. |
| 86. | Hiroyuki Koide, Ikumi Yamauchi, Yu Hoshino, Go Yasuno, Takumi Okamoto, Sotaro Akashi, Kazuhiro Saito, Naoto Oku, Tomohiro Asai, Design of abiotic polymer ligand-decorated lipid nanoparticles for effective neutralization of target toxins in the blood., Biomaterials science, 10.1039/d1bm00515d, 9, 16, 5588-5598, 2021.08, Macromolecular toxins often induce inflammatory cytokine production, multiple-organ dysfunction, and cell death. Synthetic polymer ligands (PLs) prepared with several functional monomers have the potential of neutralizing target toxins after binding to them; therefore, they are of significant interest as abiotic antidotes. Although PLs show little toxin neutralization effect in the bloodstream because of immediate elimination from there, the toxin neutralization effect is significantly improved by the direct decoration of PLs onto lipid nanoparticles (PL-LNPs). However, this direct decoration decreases PL mobility, induces LNP aggregation after capturing the target, and decreases LNP blood circulation time. We designed novel PL-LNPs to improve PL mobility, inhibit the aggregation tendency after capturing the target, and increase LNP blood circulation time in order to achieve highly effective toxin neutralization in vivo. Specifically, LNPs were modified with PLs-conjugated polyethylene glycol (PEG), and additional PEG was used to modify the PL-decorated LNPs (PL-PEG-LNPs). Histones were used as target toxins, and N-isopropylacrylamide-based PLs were used for histone capture. PEGylation increased the plasma LNP level 24 h after intravenous injection by ∼90 times and inhibited LNP aggregation after histone capture. The dissociation constant (Kd) of PL-PEG-LNPs against histone was two times smaller compared to that of PL-LNPs. Although PL-LNPs inhibited histone-platelet interaction in the bloodstream, a large amount of histone-PL-LNP complexes accumulated in the lungs because of aggregation. However, PL-PEG-LNPs inhibited both histone-platelet interaction and histone accumulation in the lungs. Importantly, PL-PEG-LNP treatment increased the survival rate of histone-treated mice compared to PL-LNPs. These results provide a platform for the development of abiotic antidote nanoparticles in vivo.. |
| 87. | Yu Hoshino, Tomohiro Gyobu, Kazushi Imamura, Akira Hamasaki, Ryutaro Honda, Ryoga Horii, Chie Yamashita, Yuki Terayama, Takeshi Watanabe, Shoma Aki, Yida Liu, Junko Matsuda, Yoshiko Miura, Ikuo Taniguchi, Assembly of Defect-Free Microgel Nanomembranes for CO2 Separation, ACS Applied Materials & Interfaces, 10.1021/acsami.1c06447, 13, 25, 30030-30038, 2021.06. |
| 88. | Takato Ishida, Masanori Nagao, Takahiro Oh, Takeshi Mori, Yu Hoshino, Yoshiko Miura, Synthesis of Glycopolymers Carrying 3′-Sialyllactose for Suppressing Inflammatory Reaction via Siglec-E, Chemistry Letters, 10.1246/cl.210740, 51, 3, 308-311, 2022.03. |
| 89. | Yusuke Saito, Ryutaro Honda, Sotaro Akashi, Hinata Takimoto, Masanori Nagao, Yoshiko Miura, Yu Hoshino, Polymer Nanoparticles with Uniform Monomer Sequences for Sequence‐Specific Peptide Recognition, Angewandte Chemie, 10.1002/ange.202206456, 134, 30, 2022.07. |
| 90. | Masanori Nagao, Yuri Kimoto, Yu Hoshino, Yoshiko Miura, Facile Preparation of a Glycopolymer Library by PET-RAFT Polymerization for Screening the Polymer Structures of GM1 Mimics, ACS Omega, 10.1021/acsomega.2c00719, 7, 15, 13254-13259, 2022.04. |
| 91. | Pisanee Srisawat, Mieko Higuchi-Takeuchi, Ryutaro Honda, Tomokazu Shirai, Akihiko Kondo, Yu Hoshino, Keiji Numata, Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria, ACS Sustainable Chemistry & Engineering, 10.1021/acssuschemeng.1c07252, 10, 13, 4133-4142, 2022.04. |
| 92. | Tomoki Imoto, Hikaru Matsumoto, Seiya Nonaka, Keita Shichijo, Masanori Nagao, Hisashi Shimakoshi, Yu Hoshino, Yoshiko Miura, 4-Amino-TEMPO-Immobilized Polymer Monolith: Preparations, and Recycling Performance of Catalyst for Alcohol Oxidation, Polymers, 10.3390/polym14235123, 14, 23, 5123-5123, 2022.11, Continuous flow reactors with immobilized catalysts are in great demand in various industries, to achieve easy separation, regeneration, and recycling of catalysts from products. Oxidation of alcohols with 4-amino-TEMPO-immobilized monolith catalyst was investigated in batch and continuous flow systems. The polymer monoliths were prepared by polymerization-induced phase separation using styrene derivatives, and 4-amino-TEMPO was immobilized on the polymer monolith with a flow reaction. The prepared 4-amino-TEMPO-immobilized monoliths showed high permeability, due to their high porosity. In batch oxidation, the reaction rate of 4-amino-TEMPO-immobilized monolith varied with stirring. In flow oxidation, the eluent permeated without clogging, and efficient flow oxidation was possible with residence times of 2–8 min. In the recycling test of the flow oxidation reaction, the catalyst could be used at least six times without catalyst deactivation.. |
| 93. | Masanori Nagao, Ai Yamaguchi, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, De Novo Design of Star-Shaped Glycoligands with Synthetic Polymer Structures toward an Influenza Hemagglutinin Inhibitor, Biomacromolecules, 10.1021/acs.biomac.1c01483, 23, 3, 1232-1241, 2022.03. |
| 94. | Hiroyuki Koide, Kazuhiro Saito, Keiichi Yoshimatsu, Beverly Chou, Yu Hoshino, Sei Yonezawa, Naoto Oku, Tomohiro Asai, Kenneth J Shea, Cooling-induced, localized release of cytotoxic peptides from engineered polymer nanoparticles in living mice for cancer therapy., Journal of controlled release : official journal of the Controlled Release Society, 10.1016/j.jconrel.2023.02.020, 355, 745-759, 2023.03, Temperature-responsive polymers are often characterized by an abrupt change in the degree of swelling brought about by small changes in temperature. Polymers with a lower critical solution temperature (LCST) in particular, are important as drug and gene delivery vehicles. Drug molecules are taken up by the polymer in their solvent swollen state below their LCST. Increasing the temperature above the LCST, typically physiological temperatures, results in desolvation of polymer chains and microstructure collapse. The trapped drug is released slowly by passive diffusion through the collapsed polymer network. Since diffusion is dependent on many variables, localizing and control of the drug delivery rate can be challenging. Here, we report a fundamentally different approach for the rapid (seconds) tumor-specific delivery of a biomacromolecular drug. A copolymer nanoparticle (NP) was engineered with affinity for melittin, a peptide with potent anti-cancer activity, at physiological temperature. Intravenous injection of the NP-melittin complex results in its accumulation in organs and at the tumor. We demonstrate that by local cooling of the tumor the melittin is rapidly released from the NP-melittin complex. The release occurs only at the cooled tumor site. Importantly, tumor growth was significantly suppressed using this technique demonstrating therapeutically useful quantities of the drug can be delivered. This work reports the first example of an in vivo site-specific release of a macromolecular drug by local cooling for cancer therapy. In view of the increasing number of cryotherapeutic devices for in vivo applications, this work has the potential to stimulate cryotherapy for in vivo drug delivery.. |
| 95. | Hiroyuki Koide, Kazuhiro Saito, Keiichi Yoshimatsu, Beverly Chou, Yu Hoshino, Sei Yonezawa, Naoto Oku, Tomohiro Asai, Kenneth J Shea, Cooling-induced, localized release of cytotoxic peptides from engineered polymer nanoparticles in living mice for cancer therapy, Journal of Controlled Release, 10.1016/j.jconrel.2023.02.020, 745, 2023.03, [URL]. |
| 96. | Tomoki Imoto, Hikaru Matsumoto, Seiya Nonaka, Keita Shichijo, Masanori Nagao, Hisashi Shimakoshi, Yu Hoshino, Yoshiko Miura, 4-Amino-TEMPO-Immobilized Polymer Monolith: Preparations, and Recycling Performance of Catalyst for Alcohol Oxidation, Polymers, 10.3390/polym14235123, e202206456, 2022.11, [URL]. |
| 97. | Yusuke Saito, Ryutaro Honda, Sotaro Akashi, Hinata Takimoto, Masanori Nagao, Yoshiko Miura, Yu Hoshino, Polymer Nanoparticles with Uniform Monomer Sequences for Sequence Specific Peptide Recognition, Angew. Chem. Int. Ed, 10.1002/ange.202206456, e202206456, 2022.05, [URL], Synthetic polymer nanoparticles (NPs) that recognize and neutralize target biomacromolecules are of considerable interest as “plastic antibodies”, synthetic mimics of antibodies. However, monomer sequences in the synthetic NPs are heterogeneous. The heterogeneity limits the target specificity and safety of the NPs. Herein, we report the synthesis of NPs with uniform monomer sequences for recognition and neutralization of target peptides. A multifunctional oligomer with a precise monomer sequence that recognizes the target peptide was prepared via cycles of reversible addition–fragmentation chain transfer (RAFT) polymerization and flash chromatography. The oligomer or blend of oligomers was used as a chain transfer agent and introduced into poly(N-isopropyl acrylamide) hydrogel NPs by radical polymerization. Evaluation of the interaction with the peptides revealed that multiple oligomers in NPs cooperatively recognized the sequence of the target peptide and neutralized its toxicity. Effect of sequence, combination, density and molecular weight distribution of precision oligomers on the affinity to the peptides was also investigated.. |
| 98. | Masanori Nagao, Yuri Kimoto, Yu Hoshino, Yoshiko Miura, Facile Preparation of a Glycopolymer Library by PET-RAFT Polymerization for Screening the Polymer Structures of GM1 Mimics, ACS Omega, 10.1021/acsomega.2c00719, 7, 15, 13254, 2022.04, [URL]. |
| 99. | Pisanee Srisawat, Mieko Higuchi-Takeuchi, Ryutaro Honda, Tomokazu Shirai, Akihiko Kondo, Yu Hoshino, Keiji Numata, Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria, ACS Sustainable Chemistry & Engineering, 10.1021/acssuschemeng.1c07252, 10, 13, 4133, 2022.03, [URL]. |
| 100. | Takato Ishida, Masanori Nagao, Takahiro Oh, Takeshi Mori, Yu Hoshino, Yoshiko Miura, Synthesis of Glycopolymers Carrying 3′-Sialyllactose for Suppressing Inflammatory Reaction via Siglec-E, Chemistry Letters, 10.1246/cl.210740, 51, 3, 308, 2022.03, [URL]. |
| 101. | T Oh, T Uemura, M Nagao, Y Hoshino, Y Miura, A QCM study of strong carbohydrate–carbohydrate interactions of glycopolymers carrying mannosides on substrates, Journal of Materials Chemistry B, 10.1039/D1TB02344F, 10, 2587, 2021.12, [URL]. |
| 102. | Masanori Nagao, Ai Yamaguchi, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, De Novo Design of Star-Shaped Glycoligands with Synthetic Polymer Structures toward an Influenza Hemagglutinin Inhibitor, Biomacromolecules, 10.1021/acs.biomac.1c01483, 23, 1232, 2021.12, [URL]. |
| 103. | Yusuke Yonamine, Takuya Asai, Yuta Suzuki, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Probing the biogenesis of polysaccharide granules in algal cells at sub-organellar resolution via Raman microscopy with stable isotope labeling, Analytical Chemistry, 10.1021/acs.analchem.1c03216, 93, 16796, 2021.12, [URL]. |
| 104. | Seiya Nonaka, Hikaru Matsumoto, Masanori Nagao, Yu Hoshino, Yoshiko Miura, Investigation of the effect of microflow reactor diameter on condensation reactions in l-proline-immobilized polymer monoliths, Reaction Chemistry & Engineering, 10.1039/D1RE00386K, 7, 55, 2021.11, [URL]. |
| 105. | Yu Hoshino, Tomohiro Gyobu, Kazushi Imamura, Akira Hamasaki, Ryutaro Honda, Ryoga Horii, Chie Yamashita, Yuki Terayama, Takeshi Watanabe, Shoma Aki, Yida Liu, Junko Matsuda, Yoshiko Miura, Ikuo Taniguchi, Assembly of Defect-Free Microgel Nanomembranes for CO2 Separation, ACS Applied Bio Materials, 3, 6, 2021.07. |
| 106. | Koide, H. Naoki Hayashi, Go Yasuno, Anna Okishima, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of synthetic polymer nanoparticles that inhibit glucose absorption from the intestine, Biochemical and Biophysical Research Communications, Doi: 10.1016/j.bbrc.2021.05.005, 561, 5, 1-6, 2021.07. |
| 107. | Hiroyuki Koide, Naoki Hayashi, Go Yasuno, Anna Okishima, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of synthetic polymer nanoparticles that inhibit glucose absorption from the intestine, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2021.05.005, 561, 1, 2021.07, [URL]. |
| 108. | Benshuai Guo, Yoshiko Miura, Yu Hoshino, Rational design of thermocells driven by the volume phase transition of hydrogel nanoparticles, ACS Applied Materials & Interfaces, 10.1021/acsami.1c07266, 13, 32184, 2021.07, [URL]. |
| 109. | Hiroyuki Koide, Ikumi Yamauchi, Yu Hoshino, Go Yasuno, Takumi Okamoto, Sotaro Akashi, Kazuhiro Saito, Naoto Oku, Tomohiro Asai, Design of abiotic polymer ligand-decorated lipid nanoparticles for effective neutralization of target toxins in the blood, Biomaterials Science, 10.1039/D1BM00515D, 268, 5588, 2021.07, [URL]. |
| 110. | Masanori Nagao, Masaya Kichize, Yu Hoshino, Yoshiko Miura, Influence of Monomer Structures for Polymeric Multivalent Ligands: Consideration of the Molecular Mobility of Glycopolymers, Biomacromolecules, 10.1021/acs.biomac.1c00553, 22, 3119, 2021.06, [URL]. |
| 111. | Ryutaro Honda, Akira Hamasaki, Yoshiko Miura, Yu Hoshino, Thermoresponsive CO 2 absorbent for various CO 2 concentrations: tuning the p K a of ammonium ions for effective carbon capture, Polymer Journal, 10.1038/s41428-020-00407-5, 53, 157-167, 2021.01, [URL]. |
| 112. | Yoshiko Miura, Yuki Kojima, Hirokazu Seto, Yu Hoshino, Bio-inert properties of TEG modified dendrimer interface, Analytical Sciences, 10.2116/analsci.20P388, 73, 3, 519-523, 2021.01, [URL].  |
| 113. | Jubao Gao, Yida Liu, Yuki Terayama, Kota Katafuchi, Yu Hoshino, Gen Inoue, Polyamine nanogel particles spray-coated on carbon paper for efficient CO2 capture in a milli-channel reactor, Chemical Engineering Journal, doi.org/10.1016/j.cej.2020.126059, 401, 126059, 2020.12. |
| 114. | Masaya Kichize, Masanori Nagao, Yu Hoshino, Yoshiko Miura, Multi-block and sequence-controlled polymerization of glycopolymers, andinteraction with lectin, Eur. Polym. J., doi.org/10.1016/j.eurpolymj.2020.110044, 140, 110044, 2020.11. |
| 115. | Takahiro Oh, Yu Hoshino, Yoshiko Miura,, Aggregation of a double hydrophilic block glycopolymer: the effect ofblock polymer ratio, J. Mat. Chemi.B, doi.org/10.1039/DoTB02093A, 8, 10101-10107, 2020.10. |
| 116. | Benshuai Guo, Yu Hoshino, Fan Gao, Keisuke Hayashi, Yoshiko Miura, Nobuo Kimizuka, Teppei Yamada, Thermocells driven by phase transition of hydrogel nanoparticles, J. Am. Chem Soc, doi.org/10.1021/jacs.0c08600, 142, 41, 17318-17322, 2020.09, Thermoelectric conversion of low temperature, delocalized, and abundant thermal sources is crucial for the development of the Internet of Things (IoT) and/or a carbon-free society. Thermocells are of great interest in thermoelectric conversion of low-temperature heat due to the low cost and flexibility of components. However, significant improvement of the conversion efficiency is required for the practical use of the cells. Here, we report thermo-electrochemical cells driven by volume phase transition (VPT) of hydrogel nanoparticles (NPs). Entropically driven VPT of poly(N-isopropylacrylamide) NPs containing carboxylic acids and amines generates a pH gradient of up to 0.049 and −0.053 pH K–1, respectively, around physiological temperature. The pH gradient triggers the proton-coupled electron transfer (PCET) reactions of quinhydrone on the electrodes, resulting in the highly efficient thermoelectric conversion with a Seebeck coefficient (Se) of −6.7 and +6.1 mV K–1. Thermocells driven by phase transition of hydrogels provide a nontoxic, flexible, and inexpensive charger that harvests carbon-free energy from abundant energy sources such as solar, body and waste heat.. |
| 117. | Hiroyuki Koide, Anna Okishima, Yu Hoshino, Yuri Kamon, Keiichi Yoshimatsu, Kazuhiro Saito, Ikumi Yamauchi, Saki Ariizumi, Yuqi Zhou, Ting-Hui Xiao, Keisuke Goda, Naoto Oku, Tomohiro Asai, Kenneth J Shea, Synthetic hydrogel nanoparticles for sepsis therapy, Nature communications, 10.1038/s41467-021-25847-2, 12, 5552, 2020.09, [URL], Sepsis is a life-threatening condition caused by the extreme release of inflammatory mediators into the blood in response to infection (e.g., bacterial infection, COVID-19), resulting in the dysfunction of multiple organs. Currently, there is no direct treatment for sepsis. Here we report an abiotic hydrogel nanoparticle (HNP) as a potential therapeutic agent for late-stage sepsis. The HNP captures and neutralizes all variants of histones, a major inflammatory mediator released during sepsis. The highly optimized HNP has high capacity and long-term circulation capability for the selective sequestration and neutralization of histones. Intravenous injection of the HNP protects mice against a lethal dose of histones through the inhibition of platelet aggregation and migration into the lungs. In vivo administration in murine sepsis model mice results in near complete survival. These results establish the potential for synthetic, nonbiological polymer hydrogel sequestrants as a new intervention strategy for sepsis therapy and adds to our understanding of the importance of histones to this condition.. |
| 118. | Yu Hoshino, Shinnosuke Shimohara, Yusuke Wada, Masahiko Nakamoto, Yoshiko Miura, Affinity Purification of Multifunctional Oligomeric Ligands Synthesizedvia Controlled Radical Polymerization, J Mat. Chem B, doi.org/10.1039/d0tb00849d, 8, 26, 5597-5601, 2020.07. |
| 119. | Hikaru Matsumoto, Yu Hoshino, Tomohiro Iwai, Masaya Sawamura, Yoshiko Miura, Polystyrene-Cross-Linking Triphenylphosphine on a Porous Monolith: EnhancedCatalytic Activity for Aryl Chloride Cross-Coupling in Biphasic Flow, Ind.Eng. Chem. Res, doi.org/10.1021/acs.iecr.0c02404, 59, 34, 15179-15187, 2020.07. |
| 120. | Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda, Raman image-activated cell sorting, Nature communications, 10.1038/s41467-020-17285-3, 11, 3452, 2020.07, [URL]. |
| 121. | Hinata Takimoto, Sho Katakami, Yoshiko Miura, Yu Hoshino, Controlling the block sequence of multi-block oligomer ligands for neutralizationof a target peptide, Materials Advances, doi.org/10.1039/DoMA00149J, 4, 604-608, 2020.06. |
| 122. | Ryutaro Honda,Tomohiro Gyobu, Hideo Shimahara, Yoshiko Miura, Yu Hoshino, Electrostatic Interactions Between Acid-/Base-Containing Polymer Nanoparticles and Proteins: Impact of Polymerization pH, ACS Applied Bio Materials, doi.org/10.1021/acsabm.0c00390, 3, 6, 3827-3834, 2020.05. |
| 123. | Hikaru Matsumoto, Yu Hoshino, Tomohiro Iwai, Masaya Sawamura, Yoshiko Miura, Polystyrene‐Supported PPh3 in Monolithic Porous Material: Effect of Cross‐LinkingDegree on Coordination Mode and Catalytic Activity in Pd‐Catalyzed C− CCross‐Coupling of Aryl Chlorides, ChemCatChem, doi.org/10.1002/cctc.202000651, 12, 16, 4034-4037, 2020.05. |
| 124. | Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Intelligent image-activated cell sorting 2.0, Lab on a Chip, 10.1039/D0LC00080A , 20, 2273, 2020.05, [URL]. |
| 125. | Yusuke Yonamine, Kotaro Hiramatsu, Takuro Ideguchi, Takuro Ito, Tomomi FujiwaraYoshiko Miura, Keisuke Goda, Yu Hoshino, Spatiotemporal monitoring of intracellular metabolic dynamics by resonanceRaman microscopy with isotope labeling, RSC Advances, doi.org/10.1039/DoRA02803G, 10, 16679-16686, 2020.04. |
| 126. | Yu Hoshino,Mitsunori Moribe, Naoki Gondo, Toshiki Jibiki, Masahiko Nakamoto, BenshuaiGuo, Rinoka Adachi, Yoshiko Miura, Combining Acid- and Base-Imprinted Nanoparticles in a Hydrogel Film forTemperature-Responsive Quick and Reversible Capture of Salt, ACS Appl. Polym. Mater, doi.org/10.1021/acsapm.9b00940, 2, 505-514, 2020.02, [URL]. |
| 127. | Yu Hoshino, Shohei Taniguchi,Hinata Takimoto, Sotaro Akashi, Sho Katakami, Yusuke Yonamine, Yoshiko Miura, Homogeneous Oligomeric Ligands Prepared via Radical Polymerization thatRecognize and Neutralize a Target Peptide, Angew. Chem. Int. Ed, doi.org/10.1002/anie.201910558, 132, 689-693, 2020.02, [URL], Abiotic ligands that bind to specific biomolecules have attracted attention as substitutes for biomolecular ligands, such as antibodies and aptamers. Radical polymerization enables the production of robust polymeric ligands from inexpensive functional monomers. However, little has been reported about the production of monodispersed polymeric ligands. Herein, we present homogeneous ligands prepared via radical polymerization that recognize epitope sequences on a target peptide and neutralize the toxicity of the peptide. Taking advantage of controlled radical polymerization and separation, a library of multifunctional oligomers with discrete numbers of functional groups was prepared. Affinity screening revealed that the sequence specificity of the oligomer ligands strongly depended on the number of functional groups. The process reported here will become a general step for the development of abiotic ligands that recognize specific peptide sequences.. |
| 128. | Yida Liu, Takashi Kodama, Taisuke Kojima, Ikuo Taniguchi, Hirokazu Seto, Yoshiko Miura, Yu Hoshino, Fine-tuning of the surface porosity of micropatterned polyethersulfonemembranes prepared by phase separation micromolding, Polymer J, doi.org/10.1038/s41428-019-0298-9, 52, 397-403, 2020.01, [URL]. |
| 129. | Yuri Kimoto, Yuhei Terada, Yu Hoshino, Yoshiko Miura, Screening of a glycopolymer library of GM1 mimics containing hydrophobic units using surface plasmon resonance imaging, ACS omega, 10.1021/acsomega.9b02877, 4, 24, 20690-20696, 2019.11. |
| 130. | Yoshiko Miura, Hirokazu Seto, Makoto Shibuya, Yu Hoshino, Biopolymer monolith for protein purification, Faraday discussions, 10.1039/C9FD00018F, 219, 154-167, 2019.11, [URL]. |
| 131. | Hiroyuki Koide, Tatsuya Fukuta, Anna Okishim, Saki Ariizumi, Chiaki Kiyokawa, Hiroki Tsuchida, Masahiko Nakamoto, Keiichi Yoshimatsu, Hidenori Ando, Takehisa Dewa, Tomohiro Asai, Naoto Oku, Yu Hoshino, Kenneth J Shea, Engineering the binding kinetics of synthetic polymer nanoparticles for siRNA delivery, Biomacromolecules, 10.1021/acs.biomac.9b00611, 20, 10, 3648-3657, 2019.09, [URL]. |
| 132. | Xinnan Cui, Yu Hoshino, Yoshiko Miura, Fibronectin Coating on Implant Material Surface Attracted Both Osteoblasts and Bacteria, Chemistry Letters, 10.1246/cl.190293, 48, 8, 764-767, 2019.08, [URL]. |
| 133. | Nobutoshi Ota, Yusuke Yonamine, Takuya Asai, Yaxiaer Yalikun, Takuro Ito, Yasuyuki Ozeki, Yu Hoshino, Yo Tanaka, Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis, Analytical chemistry, 10.1021/acs.analchem.9b01007, 91, 9631, 2019.07, [URL]. |
| 134. | Nagao,M., Matsubara, T., Hoshino, Y., Sato, T., Miura, Y., Synthesis of Various Glycopolymers Bearing Sialyllactose and the Effectof Their Molecular Mobility on Interaction with the Influenza Virus., Biomacromolecules, doi.org/10.1021/acs.biomac.9b00515, 20, 2763-2769, 2019.06, [URL]. |
| 135. | Jubao Gao, Yu Hoshino, Gen Inoue, Honeycomb-carbon-fiber-supported amine-containing nanogel particles forCO2 capture using a rotating column TVSA, Chemical Engineering Journal, doi.org/10.1016/j.cej.2019.123123, 383, 123, 2019.06, [URL]. |
| 136. | Anna Okishima, Hiroyuki Koide, Yu Hoshino, Hiromichi Egami, Yoshitaka Hamashima, Naoto Oku, Tomohiro Asai, Design of Synthetic Polymer Nanoparticles Specifically Capturing Indole, a Small Toxic Molecule, Biomacromolecules, 10.1021/acs.biomac.8b01820, 20, 4, 1644-1654, 2019.04, [URL]. |
| 137. | Yuhei Terada, Yu Hoshino, Yoshiko Miura, Glycopolymers Mimicking GM1 Gangliosides Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition, Chemistry - An Asian Journal, 10.1002/asia.201900053, 14, 7, 1021-1027, 2019.04, [URL]. |
| 138. | Masanori Nagao, Yu Hoshino, Yoshiko Miura, Quantitative preparation of multiblock glycopolymers bearing glycounits at the terminal segments by aqueous reversible addition–fragmentation chain transfer polymerization of acrylamide monomers, Journal of Polymer Science, Part A: Polymer Chemistry, 10.1002/pola.29344, 57, 8, 857-861, 2019.04, [URL]. |
| 139. | Masanori Nagao, Teruhiko Matsubara,Yu Hoshino, Toshinori Sato, and Yoshiko Miura, Topological Design of Star Glycopolymers for Controlling the Interactionwith the Influenza Virus, Bioconjugate Chemistry, 10.1021/acs.bioconjchem.9b00134, 30, 1192-1198, 2019.03, [URL]. |
| 140. | Oh, T. Jono, K. Kimoto, Y. Hoshino, Y. Miura, Y., Preparation of multifunctional glycopolymers using double orthogonal reactionsand the effect of electrostatic groups on the glycopolymer–lectin interaction, Polymer Journal, doi.org/10.1038/s41428-019-0244-x, 51, 1299-1308, 2019.03, [URL]. |
| 141. | Hiroyuki Koide, Keiichi Yoshimatsu, Yu Hoshino, Saki Ariizumi, Anna Okishima, Takafumi Ide, Hiromichi Egami, Yoshitaka Hamashima, Yuri Nishimura, Hiroaki Kanazawa, Yoshiko Miura, Tomohiro Asai, Naoto Oku, Kenneth J. Shea, Sequestering and inhibiting a vascular endothelial growth factor in vivo by systemic administration of a synthetic polymer nanoparticle, Journal of Controlled Release, 10.1016/j.jconrel.2018.12.033, 295, 13-20, 2019.02, [URL]. |
| 142. | Koide, H. Yoshimatsu, K. Hoshino, Y. Ariizumi, S. Okishima,A. Ide, T. Egami, H. Hamashima, Y. Nishimura, Y. Kanazawa, H. Miura, Y.Asai, T. Oku, N. Shea, K.J., Sequestering and inhibiting a vascular endothelial growth factorin vivo by systemic administration of a synthetic polymer nanoparticle , Journal of Controlled Release, Doi: 10.1016/j.jconrel.2018.12.033, 295, 13-20, 2019.02. |
| 143. | Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, Sang Wook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda, High-throughput label-free molecular fingerprinting flow cytometry, Science Advances, 10.1126/sciadv.aau0241, 5, 1, 2019.01, [URL]. |
| 144. | Hikaru Matsumoto, Takanori Akiyoshi, Yu Hoshino, Hirokazu Seto, Yoshiko Miura, Size-tuned hydrogel network of palladium-confining polymer particles a highly active and durable catalyst for Suzuki coupling reactions in water at ambient temperature, Polymer Journal, 10.1038/s41428-018-0102-2, 50, 12, 1179-1186, 2018.12, [URL]. |
| 145. | Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, Sang Wook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda, Intelligent Image-Activated Cell Sorting, Cell, 10.1016/j.cell.2018.08.028, 175, 1, 266-276.e13, 2018.09, [URL], A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machinebased scientific discovery in biological, pharmaceutical, and medical sciences.. |
| 146. | Yu Hoshino, Toshiki Jibiki, Masahiko Nakamoto, Yoshiko Miura, Reversible p Ka Modulation of Carboxylic Acids in Temperature-Responsive Nanoparticles through Imprinted Electrostatic Interactions, ACS Applied Materials and Interfaces, 10.1021/acsami.8b11397, 10, 37, 31096-31105, 2018.09, [URL]. |
| 147. | Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda, A practical guide to intelligent image-activated cell sorting, Nature protocols, 10.1038/s41596-019-0183-1, 14, 2370, 2018.08, [URL]. |
| 148. | Xinnan Cui, Tatsuya Murakami, Yukihiko Tamura, Kazuhiro Aoki, Yu Hoshino, Yoshiko Miura, Bacterial Inhibition and Osteoblast Adhesion on Ti Alloy Surfaces Modified by Poly(PEGMA- r-Phosmer) Coating, ACS Applied Materials and Interfaces, 10.1021/acsami.8b07757, 10, 28, 23674-23681, 2018.07, [URL]. |
| 149. | Takahiro Oh, Masanori Nagao, Yu Hoshino, Yoshiko Miura, Self-Assembly of a Double Hydrophilic Block Glycopolymer and the Investigation of Its Mechanism, Langmuir, 10.1021/acs.langmuir.8b01527, 34, 29, 8591-8598, 2018.07, [URL]. |
| 150. | Yu Hoshino, Temperature-responsive on-off switching of target recognition by nanogel particles, Kobunshi, 67, 5, 253-254, 2018.05. |
| 151. | Xinnan Cui, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Anti-biofouling phosphorylated HEMA and PEGMA block copolymers show high affinity to hydroxyapatite, Colloids and Surfaces B: Biointerfaces, 10.1016/j.colsurfb.2017.09.038, 160, 289-296, 2017.12, [URL]. |
| 152. | Masanori Nagao, Yurina Fujiwara, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura, Design of Glycopolymers Carrying Sialyl Oligosaccharides for Controlling the Interaction with the Influenza Virus, Biomacromolecules, 10.1021/acs.biomac.7b01426, 18, 12, 4385-4392, 2017.12, [URL]. |
| 153. | Hiroyuki Koide, Hiroki Tsuchida, Masahiko Nakamoto, Anna Okishima, Saki Ariizumi, Chiaki Kiyokawa, Tomohiro Asai, Yu Hoshino, Naoto Oku, Rational designing of an antidote nanoparticle decorated with abiotic polymer ligands for capturing and neutralizing target toxins, Journal of Controlled Release, 10.1016/j.jconrel.2017.10.028, 268, 335-342, 2017.12, [URL]. |
| 154. | Yoshiko Miura, Tomohiro Fukuda, Hirokazu Seto, Yu Hoshino, Syntheses and functions of Glycosaminoglycan mimicking polymers, Coupling and Decoupling of Diverse Molecular Units in Glycosciences, 10.1007/978-3-319-65587-1_10, 213-224, 2017.11, [URL]. |
| 155. | Yusuke Yonamine, Yuta Suzuki, Takuro Ito, Yoshiko Miura, Keisuke Goda, Yasuyuki Ozeki, Yu Hoshino, Monitoring Photosynthetic Activity in Microalgal Cells by Raman Spectroscopy with Deuterium Oxide as a Tracking Probe, ChemBioChem, 10.1002/cbic.201700314, 18, 20, 2063-2068, 2017.10, [URL]. |
| 156. | Hiromu Kashida, Chie Kojima, Takamasa Sakai, Takaya Terashima, Keiji Numata, Yu Hoshino, Hiroshi Yabu, Kotaro Satoh, The coming SPSJ under the changing environment of R&D, Kobunshi, 66, 4, 167-174, 2017.04. |
| 157. | H. Koide, K. Yoshimatsu, Y. Hoshino, S.-H. Lee, A. Okajima, S. Ariizumi, Y. Narita, Y. Yonamine, A. C. Weisman, Y. Nishimura, N. Oku, Y. Miura, K. J. Shea, A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF165), Nat. Chem., 10.1038/nchem.2749, 2017.03. |
| 158. | M. Yue, K. Imai, C. Yamashita, Y. Miura, Y. Hoshino, Effects of Hydrophobic Modifications and Phase Transitions of Polyvinylamine Hydrogel Films on Reversible CO2 Capture Behavior: Comparison between Copolymer Films and Blend Films for Temperature‐Responsive CO2 Absorption, Macromol. Chem. and Phys., 10.1002/macp.201600570, 218, 1600570, 2017.02. |
| 159. | K. Jono, M. Nagao, T. Oh, S. Sonoda, Yu Hoshino, Yoshiko Miura, Controlling the lectin recognition of glycopolymers Via distance arrangement of sugar blocks, Chemical Communications, 10.1039/c7cc07107h, 54, 1, 82-85, 2017.01, [URL]. |
| 160. | Hikaru Matsumoto, Hirokazu Seto, Takanori Akiyoshi, Makoto Shibuya, Yu Hoshino, Yoshiko Miura, Macroporous gel with a permeable reaction platform for catalytic flow synthesis, ACS Omega, 10.1021/acsomega.7b00909, 2, 12, 8796-8802, 2017.01, [URL]. |
| 161. | Yu Hoshino, Takaaki Miyoshi, Masahiko Nakamoto, Yoshiko Miura, Wide-range p K a tuning of proton imprinted nanoparticles for reversible protonation of target molecules via thermal stimuli, Journal of Materials Chemistry B, 10.1039/c7tb02107k, 5, 46, 9204-9210, 2017.01, [URL]. |
| 162. | Xinnan Cui, Hirokazu Seto, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Inhibition of Bacterial Adhesion on Hydroxyapatite Model Teeth by Surface Modification with PEGMA-Phosmer Copolymers, ACS Biomater. Sci. Eng, 10.1021/acsbiomaterials.5b00349, 2, 2, 205-212, 2016.02. |
| 163. | Yoshiko Miura, Yu Hoshino, Hirokazu Seto, Glycopolymer Nanobiotechnology, Chemical Reviews, 10.1021/acs.chemrev.5b00247, 116, 1673-1692, 2016.02. |
| 164. | Masanori Nagao, Yuuki Kurebayashi, Hirokazu Seto, Tomonari Tanaka, Tadanobu Takahashi, Takashi Suzuki, Yu Hoshino, Yoshiko Miura, Synthesis of well-controlled glycopolymers bearing oligosaccharides and their interactions with influenza viruses, Polymer Journal, 2016.02. |
| 165. | Masahiko Nakamoto, Tadashi Nonaka, Yoshiko Miura, Kenneth J. Shea, Yu Hoshino*, Design of Synthetic Polymer Nanoparticles that Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme, J. Am. Chem. Soc., 10.1021/jacs.5b12600, 138, 2016.02, [URL]. |
| 166. | Hirokazu Seto, Kenta Imai, Yu Hoshino, Yoshiko Miura, Polymer microgel particles as basic catalysts for Knoevenagel condensation in water, Polymer Journal, 2016.02. |
| 167. | Yoshiko Miura, Tomohiro Fukuda, Hirokazu Seto, Yu Hoshino, Development of glycosaminoglycan mimetics using glycopolymers, Polymer Journal, 10.1038/pj.2015.110, 2015.11. |
| 168. | Lee Haejoo, Yu Hoshino*, Yusuke Wada, Yuka Arata, Atsushi Maruyama, Yoshiko Miura, Minimization of synthetic polymer ligands for specific recognition and neutralization of a toxic peptide, J. Am. Chem. Soc., 10.1021/jacs.5b05259, 137, 10878-10881, 2015.09, [URL]. |
| 169. | Mengchen Yue, Yu Hoshino*, Yoshiko Miura, Design Rationale of Thermally Responsive Microgel Particle Films That Reversibly Absorb Large Amounts of CO2: Fine Tuning the pKa of Ammonium Ions in the Particles, Chem. Sci., 10.1039/C5SC01978H, ASAP, 2015.08, [URL]. |
| 170. | Hidekazu Seto, Seiji Kamba, Takashi Kondo, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody, Anal. Sci., 10.2116/analsci.31.173, 31, 173-176, 2015.05, [URL]. |
| 171. | Keiichi Yoshimatsu, Hiroyuki Koide, Yu Hoshino, Kenneth J. Shea, Preparation of abiotic polymer nanoparticles for sequestration and neutralization of a target peptide toxin, Nature protocols, 10.1038/nprot.2015.032, 10, 595–604, 2015.04, [URL]. |
| 172. | Yu Hoshino*, Yuka Arata, Haejoo Lee, Yusuke Yonamine, Shih-Hui Lee, Aki Yamasaki, Ryousuke Tsuhara, Katsuhiko Yano, Kenneth J Shea, Yoshiko Miura, Preparation of nanogel-immobilized porous gel beads for affinity separation of proteins: fusion of nano and micro gel materials, Polymer Journal, 10.1038/pj.2014.101, 47, 220–225, 2015.01, [URL]. |
| 173. | Yusuke Wada, Haejoo Lee, Yu Hoshino*, Shunsuke Kotani, Kenneth J. Shea, Yoshiko Miura, Design of multi-functional linear polymers that capture and neutralize a toxic peptide: a comparison with cross-linked nanoparticles, Journal of Materials Chemistry B, 10.1039/C4TB01967A, 3, 1706-1711, 2015.01, [URL]. |
| 174. | Yoke-Ming Wong, Yu Hoshino*, Kumar Sudesh, Yoshiko Miura, Keiji Numata*, Optimization of poly (N-isopropylacrylamide) as an artificial amidase, Biomacromolecules, 10.1021/bm501671r, 16, 411–421, 2015.01, [URL]. |
| 175. | Yu Hoshino, LEE HAEJOO, Yoshiko Miura, Interaction between synthetic particles and biomacromolecules: fundamental study of nonspecific interaction and design of nanoparticles that recognize target molecules, Polymer Journal, 10.1038/pj.2014.33, 46, 537–545, 2014.10, [URL]. |
| 176. | Hidekazu Seto, Tamami Yoneda, Takato Morii, Yu Hoshino, Yoshiko Miura, Tatsuya Murakami, Membrane reactor immobilized with palladium‐loaded polymer nanogel for continuous‐flow Suzuki coupling reaction, AIChE Journal, 10.1002/aic.14653, 61, 582–589, 2014.10, [URL]. |
| 177. | Hidekazu Seto, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Shigeki Nashima, Yoshinobu Ehara, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Metal Mesh Device Sensor Immobilized with a Trimethoxysilane-Containing Glycopolymer for Label-Free Detection of Proteins and Bacteria, ACS applied materials & interfaces, 10.1021/am503003v, 6, 15, 13234-13241, 2014.08, [URL]. |
| 178. | Adam Weisman, Yingyao Allie Chen, Yu Hoshino, Huiting Zhang, Kenneth J. Shea, Engineering Nanoparticle Antitoxins Utilizing Aromatic Interactions, Biomacromolecules, 10.1021/bm500666j, 15, 3290–3295, 2014.06, [URL]. |
| 179. | Yu Hoshino*, Ryohei C. Ohashi, Yoshiko Miura, Rational Design of Synthetic Nanoparticles with a Large Reversible Shift of Acid Dissociation Constants: Proton Imprinting in Stimuli Responsive Nanogel Particles, Advanced Mater, 10.1002/adma.201305957, 26, 2449–2606, 2014.03, [URL]. |
| 180. | Yuhei Terada, Wakana Hashimoto, Tatsuro Endo, Hidekazu Seto, Tatsuya Murakami, Hideaki Hisamoto, Yu Hoshino, Yoshiko Miura, Signal amplified two-dimensional photonic crystal biosensor immobilized with glyco-nanoparticles, J. Mater. Chem. B, 10.1039/C4TB00028E, in press, 2014.02, [URL]. |
| 181. | K. Yoshimatsu, T. Yamazaki, Yu Hoshino, L. F. Epstein, L. P. Miranda, P. Tagari, J. M. Beierle, Y. Ynamine, K. J. Shea, Epitope Discovery for a Synthetic Polymer Nanoparticle: A New Strategy for Developing a Peptide Tag, J. Am. Chem. Soc., 10.1021/ja410817p, 136, 1194–1197, 2014.01, [URL]. |
| 182. | Mengchen Yue, Yu Hoshino*, Yukinori Ohshiro, Kazushi Imamura, Yoshiko Miura, Temperature-Responsive Microgel Films as Reversible Carbon Dioxide Absorbents in Wet Environment, Angew. Chem. Int. Ed., 10.1002/ange.201309758, 126, 2692–2695, 2014.01, [URL]. |
| 183. | Yuri Nishimura, Hiroki Shudo, Hidekazu Seto, Yu Hoshino, Yoshiko Miura, Syntheses of sulfated glycopolymers and analyses of their BACE-1 inhibitory activity, Bioorganic & Medicinal Chemistry Letters, 10.1016/j.bmcl.2013.09.057, 23, 6390–6395, 2013.12, [URL]. |
| 184. | Masahiko Nakamoto, Yu Hoshino*, Yoshiko Miura*, Effect of Physical Properties of Nanogel Particles on the Kinetic Constants of Multipoint Protein Recognition Process, Biomacromolecules, 10.1021/bm401536v, 15, 541–547, 2013.12, [URL]. |
| 185. | Seto Hidekazu, Seiji Kamba, Takashi Kondo, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Novel Detection Technique for Particulate Matter in Air Using Metal Mesh Device Sensors, Chem. Lett., 10.1246/cl.131040, 43, 408-410, 2013.11, [URL]. |
| 186. | Masaki Takara, Masayuki Toyoshima, Hidekazu Seto, Yu Hoshino, Yoshiko Miura, Polymer-modified gold nanoparticles via RAFT polymerization: a detailed study for a biosensing application, Polym. Chem., 10.1039/C3PY01001E, 5, 931-939, 2013.09, [URL]. |
| 187. | Seto Hidekazu, Chie Yamashita, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Mitsuhiro Matsuno, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura, Biotinylation of Silicon and Nickel Surfaces and Detection of Streptavidin as Biosensor, Langmuir, 10.1021/la401068n, 29, 9457–9463, 2013.07, [URL]. |
| 188. | Y. Ogata, Hidekazu Seto, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura, Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine, Membranes, 10.3390/membranes3030169, 3, 169-181, 2013.07, [URL]. |
| 189. | Seto, Hidekazu, Morii, Takato, Yoneda, Tamami, Murakami, Tatsuya, Yu Hoshino, Yoshiko Miura, Preparation of Palladium-loaded Polymer Nanoparticles with Catalytic Activity for Hydrogenation and Suzuki Coupling Reactions, Chem. Lett. , 10.1246/cl.2013.301, 42, 3, 301-303, 2013.03, [URL]. |
| 190. | Yonamine, Yusuke, Yoshimatsu, Keiichi, Lee, Shih-Hui, Yu Hoshino, Okahata, Yoshio, Shea, Kenneth J, Polymer Nanoparticle-Protein Interface. Evaluation of the Contribution of Positively Charged Functional Groups to Protein Affinity, ACS APPLIED MATERIALS & INTERFACES, 10.1021/am302404q, 5, 2, 374-379, 2013.01. |
| 191. | Y. Hoshino*, K. Imamura , M. Yue , G. Inoue , Y. Miura*, Reversible absorption of CO2 triggered by phase transition of amine-containing micro- and nano-gel particles, J. Am. Chem. Soc., 10.1021/ja3080192, 134, 18177-18180, 2012.10, [URL]. |
| 192. | H. Seto , M. Takara , C. Yamashita , T. Murakami , T. Hasegawa , Y. Hoshino , and Y. Miura, Surface Modification of Siliceous Materials Using Maleimidation and Various Functional Polymers Synthesized by Reversible Addition Fragmentation Chain Transfer Polymerization, ACS Appl. Mater. Interfaces, 10.1021/am301637q, 4, 5125–5133, 2012.09, [URL]. |
| 193. | Y. Hoshino*, M. Nakamoto, and Y. Miura*, Control of protein-binding kinetics on synthetic polymer nanoparticles by tuning flexibility and inducing conformation changes of polymer chains, J. Am. Chem. Soc., 10.1021/ja306053s, 134, 15209−15212, 2012.09, [URL]. |
| 194. | S.-H. Lee, Y. Hoshino, A. Randall, Z. Zeng, P. Baldi, R.-a. Doong and K. J. Shea, Engineered Synthetic Polymer Nanoparticles as IgG Affinity Ligands, J. Am. Chem. Soc., 10.1021/ja303612d, 134, 15765−15772, 2012.09, [URL]. |
| 195. | Y. Yonamine, Y. Hoshino, and K. J. Shea, An ELISA-mimic screen for synthetic polymer nanoparticles with high affinity to target proteins, Biomacromolecules, 10.1021/bm300986j, 13, 2952–2957, 2012.07, [URL]. |
| 196. | K. Yoshimatsu, B. K. Lesel, Y. Yonamine, J. M. Beierle, Y. Hoshino, and K. J. Shea, Temperature-Responsive “Catch and Release” of Proteins by using Multifunctional Polymer-Based Nanoparticles, Angew. Chem. Int. Ed., 10.1002/anie.201107797, 51, 2405–2408, 2012.03, [URL]. |
| 197. | Y. Hoshino, M. Nakamoto, Y. Miura, Influence of Polymer Density of Plastic Antibodies on Target Binding Kinetics, Polymer Preprint, 53, 656-657, 2012.03. |
| 198. | H. Seto, Y. Ogata, T. Murakami, Y. Hoshino, and Y. Miura, Selective Protein Separation Using Siliceous Materials with a Trimethoxysilane-Containing Glycopolymer, ACS Appl. Mater. Interfaces, 10.1021/am2014713, 4, 411-417, 2012.01, [URL]. |
| 199. | Y. Hoshino*, H. Koide, K. Furuya, W. W. Haberaecker III, S. Lee, T. Kodama, H. Kanazawa, N. Oku, and K. J. Shea*, The Rational Design of a Synthetic Polymer Nanoparticles that Neutralizes a Toxic Peptide in Vivo, Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1112828109, 109, 33-38, 2012.01, [URL]. |
| 200. | Keiichi YOSHIMATSU, Ben LESEL, Yu HOSHINO, Kenneth J. SHEA, Interactions between Designed Synthetic Polymer Nanoparticles and a Toxic Peptide: Toward Understanding of Factors behind the High Affinity, Polymer Preprints, 52, 486, 2011.03. |
| 201. | Yusuke YONAMINE, Tomoki YOKOYAMA, Kosuke SHIMIZU, Yu HOSHINO, Naoto OKU, Kenneth J. SHEA, Synthetic Polymer Nanoparticles that Capture the Antigen Protein of Japanese Cedar Pollen Allergy, Polymer Preprints, 52, 490, 2011.03. |
| 202. | Masaya WADA, Yuta MIYAZAWA, Yu HOSHINO, Yoshiko MIURA, Specific Biological Ability of Trehalose and Multivalent Trehalose on Aβ Aggregation, Polymer Preprints, 52, 491-492, 2011.03. |
| 203. | Shih-Hui LEE, Yu HOSHINO, Ruey-an DOONG, Kenneth J. SHEA, Development of Plastic Nanoparticles that Capture Antibodies: “Plastic Protein A”, Polymer Preprints, 52, 502-503, 2011.03. |
| 204. | Yu HOSHINO, Hiroyuki KOIDE, Dai OYAMA, Yusuke YONAMINE, Shih-Hui LEE, Naoto OKU, Kenneth J. SHEA, Design of Polymer Nanoparticles that are Capable of Neutralizing Toxicity of Fetal Proteins, Polymer Preprints, 52, 591, 2011.03. |
| 205. | Y. Hoshino, and K. J. Shea*, Evolution of Plastic Antibodies, J. Mat. Chem., 2010.10, [URL]. |
| 206. | Y. Hoshino*, W. W. Haberaecker III, T. Kodama, Z. Zeng, Y. Okahata, and K. J. Shea*, Affinity Purification of Multifunctional Polymer Nanoparticles, J. Am. Chem. Soc., 10.1021/ja1058982, 132, 13648-13650, 2010.09, [URL]. |
| 207. | Y. Hoshino*, H. Koide, T. Urakami, H. Kanazawa, T. Kodama, N. Oku, and K. J. Shea*, Recognition, Neutralization, and Clearance of Target Peptides in the Bloodstream of Living Mice by Molecularly Imprinted Polymer Nanoparticles: A Plastic Antibody, J. Am. Chem. Soc., 10.1021/ja102148f, 132, 6644–6645, 2010.04, [URL]. |
| 208. | Z. Zeng, Y. Hoshino, A. Rodoriguez, H. Yoo, and K. J. Shea, Synthetic Polymer Nanoparticles with Antibody-Like Affinity for a Hydrophilic Peptide, ACS nano, 10.1021/nn901256s, 4, 199–204, 2009.12, [URL]. |
| 209. | K. J. Shea and Y. Hoshino , Molecularly Imprinted Polymers. Synthetic Receptors for Small Molecules, Peptides and Proteins. The Evolution of a “Plastic Antibody”, MedChem News, 19, 12-16, 2009.08, [URL], 分子インプリント法により抗体と同様の大きさ、分子認識能力を有するプラスチック抗体を合成できることを示した論文。. |
| 210. | T. Kawasaki, M. Toyoda, Y. Hoshino, and Y. Okahata , Pulsed Ultrasound Effect on DNA Polymerase Reaction Monitored on a QCM, Chem. Lett. , 10.1246/cl.2009.538, 38, 538-539, 2009.03, [URL]. |
| 211. | Y. Hoshino*, T. Urakami, T. Kodama, H. Koide, N. Oku, Y. Okahata, and K. J. Shea*, Design of Synthetic Polymer Nanoparticles that Capture and Neutralize Toxic Peptide, Small, 10.1002/smll.200900186, 5, 1562–1568, 2009.03. |
| 212. | Y. Hoshino, T. Kodama, Y. Okahata, and K. J. Shea, Peptide Imprinted Polymer nanoparticles “Plastic Antibodies”, J. Am. Chem. Soc., 10.1021/ja8062875, 130, 15242-15243, 2008.10, [URL]. |
| 213. | Y. Hoshino, T. Kawasaki, and Y. Okahata, Effect of Ultrasound on DNA Polymerase Reactions: Monitoring on a 27-MHz Quartz Crystal Microbalance, Biomacromolecules, 10.1021/bm050738e, 7, 682–685, 2006.02, [URL]. |
| 214. | T. Kawasaki, Y. Hoshino, Y. Ishizu, Y. Mizushiro, and Y. Okahata, Control of Hydrolysis and Condensation Activities of Thermolysin by Ultrasound Irradiation, Chem. Lett., 10.1246/cl.2005.1602, 34, 1602-1603, 2005.09, [URL]. |
| 215. | Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata, A RNA-aligned Film Prepared from a RNA-Lipid Complex, Macromol. Rapid Commun., 10.1002/1521-3927(20020301)23:4, 23, 253-255, 2002.03, [URL]. |
| 216. | Y. Hoshino, H. Nakayama, and Y. Okahata, Preparations of a RNA-lipid Complex Film and Its Physical Properties, Nucleic Acids Res. Suppl., 10.1093/nass/1.1.61, 1, 61-62, 2001.10, [URL]. |
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