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Baba Takashi Last modified date:2024.06.03

Associate Professor / Sex differences (Molecular Biology)
Department of Molecular Biology
Faculty of Medical Sciences

Graduate School

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Academic Degree
Ph. D
Country of degree conferring institution (Overseas)
Field of Specialization
Molecular Biology
ORCID(Open Researcher and Contributor ID)
Total Priod of education and research career in the foreign country
Outline Activities
My research interests are as below.
1. Elucidation of the mechanism establishing whole body sex by corticosterone secreted from adrenal glands.
2. Elucidation of the molecular mechanism underlying the regulation of energy metabolism by nuclear receptors.
Research Interests
  • Elucidation of sex-specific functions of adrenal cortex cells.
    keyword : Adrenal cortex, Sex difference
  • Elucidation of the mechanism inducing sex differences in energy metabolism in skeletal muscle.
    keyword : Skeletal muscle, sex difference
  • Identification of sex differences in chromatin structure established by histone demethylases encoded by sex chromosomes.
    keyword : sex chromosome, histone demethylase (Utx, Uty, Smcx, Smcy)
  • Energy metabolism regulated by a nuclear receptor, Ad4BP.
    keyword : Metabolism
  • Regulation of cellular energy production by nuclear receptor, Ad4BP/SF-1.
    keyword : glycolysis, regulation of gene expression, energy production
  • Identification of mechanism of the establishment of sex differences
    keyword : Chromatin modification, sex difference
Academic Activities
1. Fumiya Takahashi, Takashi Baba* Antonius Christianto Shogo Yanai, Hyeon-Cheol Lee-Okada, Keisuke Ishiwata, Kazuhiko Nakabayashi, Kenichiro Hata, Tomohiro Ishii, Tomonobu Hasegawa, Takehiko Yokomizo, Man Ho Choi, and Ken-ichirou Morohashi, Development of sexual dimorphism of skeletal muscles through the adrenal cortex, caused by androgen-induced global gene suppression, Cell Reports,, 43, 113715, 2024.02.
2. Fumiya Takahashi, Takashi Baba, Antonius Christianto, Shogo Yanai, Keisuke Ishiwata, Kazuhiko Nakabayashi, Kenichiro Hata, Tomohiro Ishii, Tomonobu Hasegawa, Man Ho Choi, Ken-ichirou Morohashi, Development of Sexual Dimorphism Through the Adrenal Cortex, Caused by Androgen-Induced Global Gene Suppression, Sneak Peek, 10.2139/ssrn.4425878, 2023.04, The zona fasciculata (zF) in the adrenal cortex contributes to multiple physiological actions through glucocorticoid synthesis. The size, proliferation, and glucocorticoid synthesis characteristics are all fe- male biased, and sexual dimorphism is established by androgen. In this study, transcriptomes were ob- tained to unveil the sex differentiation mechanism. Interestingly, both the amount of mRNA and the ex- pressions of nearly all genes were higher in females. The expression of Nr5a1, which is essential for steroidogenic cell differentiation, was also female biased. Whole-genome studies demonstrated that NR5A1 regulates nearly all gene expression directly or indirectly. This suggests that androgen-induced global gene suppression is potentially mediated by NR5A1. Using Nr5a1 heterozygous mice, whose ad- renal cortex is smaller than the wild type, we demonstrated that the size of skeletal muscles is possibly regulated by glucocorticoid synthesized by zF. Taken together, considering the ubiquitous presence of glucocorticoid receptors, our findings provide a pathway for sex differentiation through glucocorticoid synthesis..
3. Yanai S, Baba T, Inui K, Miyabayashi K, Han S, Inoue M, Takahashi F, Kanai Y, Ohkawa Y, Choi MH, Morohashi KI., Gene expression and functional abnormalities in XX/Sry Leydig cells, SCIENTIFIC REPORTS, 10.1038/s41598-020-80741-z, 11, 1, 2021.01.
4. Baba T, Otake H, Inoue M, Sato T, Ishihara Y, Moon JY, Tsuchiya M, Miyabayashi K, Ogawa H, Shima Y, Wang L, Sato R, Yamazaki T, Suyama M, Nomura M, Choi MH, Ohkawa Y, Morohashi KI., Ad4BP/SF-1 regulates cholesterol synthesis to boost the production of steroids, Communications Biology, 10.1038/s42003-018-0020-z, 18, 2018.02.
5. Takashi Baba, Hiroyuki Otake, Tetsuya Sato, Kanako Miyabayashi, Yurina Shishido, Chia-Yih Wang, Yuichi Shima, Hiroshi Kimura, Mikako Yagi, Yasuhiro Ishihara, Shinjiro Hino, Hidesato Ogawa, Mitsuyoshi Nakao, Takeshi Yamazaki, Dongchon Kang, Yasuyuki Ohkawa, Mikita Suyama, Bon-Chu Chung, Ken-Ichirou Morohashi, Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1, Nature Communications, 10.1038/ncomms4634, 5, 3634-3634, 2014.04, Genetic deficiencies in transcription factors can lead to the loss of certain types of cells and tissue. The steroidogenic tissue-specific nuclear receptor Ad4BP/SF-1 (NR5A1) is one such gene, because mice in which this gene is disrupted fail to develop the adrenal gland and gonads. However, the specific role of Ad4BP/SF-1 in these biological events remains unclear. Here we use chromatin immunoprecipitation sequencing to show that nearly all genes in the glycolytic pathway are regulated by Ad4BP/SF-1. Suppression of Ad4BP/SF-1 by small interfering RNA reduces production of the energy carriers ATP and nicotinamide adenine dinucleotide phosphate, as well as lowers expression of genes involved in glucose metabolism. Together, these observations may explain tissue dysgenesis as a result of Ad4BP/SF-1 gene disruption in vivo. Considering the function of estrogen-related receptor a, the present study raises the possibility that certain types of nuclear receptors regulate sets of genes involved in metabolic pathways to generate energy carriers..
6. Keiko Nohara, Takashi Baba, Hikari Murai, Yayoi Kobayashi, Takehiko Suzuki, Yukiyo Tateishi, Michiyo Matsumoto, Noriko Nishimura, and Tomoharu Sano, Genomic DNA methylation in the mouse liver is affected by methyl deficiency and arsenic in a sex- and a dietary-fat-content-dependent manner, Arch. Toxicol., 85, 653-661, 2011.01.
7. Baba, T.; Shima, Y.; Owaki, A.; Mimura, J.; Oshima, M.; Fujii-Kuriyama, Y.; Morohashi, K. -i., Disruption of aryl hydrocarbon receptor (AhR) induces regression of the seminal vesicle in aged male mice, Sex. Dev., 10.1159/000117714, 2, 1, 1-11, 2008.01.
8. Takashi Baba, Junsei Mimura, Naohito Nakamura, Nobuhiro Harada, Masayuki Yamamoto, Ken-Ichirou Morohashi, Yoshiaki Fujii-Kuriyama, Intrinsic function of the aryl hydrocarbon (dioxin) receptor as a key factor in female reproduction., Molecular and cellular biology, 25, 22, 10040-51, 2005.11, Dioxins exert a variety of adverse effects on organisms, including teratogenesis, immunosuppression, tumor promotion, and estrogenic action. Studies using aryl hydrocarbon receptor (AhR)-deficient mice suggest that the majority of these toxic effects are mediated by the AhR. In spite of the adverse effects mediated by this receptor, the AhR gene is conserved among a number of animal species, ranging from invertebrates to vertebrates. This high degree of conservation strongly suggests that AhR possesses an important physiologic function, and a critical function is also supported by the reduced fertility observed with AhR-null female mice. We demonstrate that AhR plays a crucial role in female reproduction by regulating the expression of ovarian P450 aromatase (Cyp19), a key enzyme in estrogen synthesis. As revealed by in vitro reporter gene assay and in vivo chromatin immunoprecipitation assay, AhR cooperates with an orphan nuclear receptor, Ad4BP/SF-1, to activate Cyp19 gene transcription in ovarian granulosa cells. Administration to female mice of an AhR ligand, DMBA (9,10-dimethyl-1,2-benzanthracene), induced ovarian Cyp19 gene expression, irrespective of the intrinsic phase of the estrus cycle. In addition to elucidating a physiological function for AhR, our studies also suggest a possible mechanism for the toxic effects of exogenous AhR ligands as endocrine disruptors..
1. Fumiya Takahashi, Takashi Baba, Ken-ichirou Morohashi, Development of sexual dimorphism through the adrenal cortex, caused by androgen-induced global gene suppression, 9th International Conference of Brain and Gonadal Biology, 2023.10.
2. Takashi Baba, Yoshihiro Izumi, Keisuke Tomohara, Masatomo Takahashi, Fumiya Takahashi, Antonius Christianto, Hiroki Yamazaki, Takeru Nose, Takeshi Bamba & Ken-ichirou Morohashi, Absolute quantification of fructose-2,6-bisphosphate, a regulator of glycolysis, 9th International Conference of Brain and Gonadal Biology, 2023.10.
3. Takashi Baba, Deciphering Cellular Sex difference: Insights from Skeletal Muscle and the Adrenal Glands, 5th International Seminar on Smart Molecules of Natural Resources, 2023.10.
4. Takashi Baba, Hiroyuki Otake, Miki Inoue, Kanako Miyabayashi, Yuichi Shima, Tetsuya Sato, Yasuyuki Ohkawa, Mikita Suyama, Ken-ichirou Morohashi., ORCHESTRATION OF HOUSEKEEPING AND CELL-SPECIFIC METABOLISM FOR STEROID HORMONE SYNTHESIS BY A SINGLE TRANSCRIPTION FACTOR, Ad4BP/SF-1., 8th International Symposium on the Biology of Vertebrate Sex Determination, 2018.04.
5. 馬場 崇、Bing Li、諸橋 憲一郎, Role of Ad4-Binding Protein/Steroidogenic Factor-1 (Ad4BP/SF-1) in regulating NADPH production in adrenocortical Y-1 cells, Asian Sex Differentiation Network (7th Gonad Biology Joint Meeting), 2017.10.
6. 馬場 崇, 諸橋 憲一郎, Regulation of de novo cholesterol synthesis by a nuclear receptor Ad4BP/SF-1, XVIIth Adrenal Cortex Conference, 2016.03.
7. 馬場 崇, Nuclear receptor and metabolism -Rediscovery of the "Regulon" system-, 19th International Conference on Cytochrome P450, 2015.06.
8. 馬場 崇, Identification of Ad4BP/SF-1 target genes by mRNA-seq and ChIP-seq in Y-1 adrenocortical cells, 6th International Symposium on the Biology of Vertebrate Sex Determination, 2012.04.
Membership in Academic Society
  • American Society for Biochemistry and Molecular Biology
  • JBC/Herbert Tabor Young Investigator Award
  • Dr. Baba gave a presentation entitled “Nuclear Receptors and Metabolism” in the 19th. International Symposium on Cytochrome P450 and received this award.