Kyushu University [Ohuchida Kenoki (Associate Professor) Faculty of Medical Sciences, Department of Clinical Medicine]

Ohuchida Kenoki

Last modified date：2024.04.17

Associate Professor / Department of Surgery and Oncology / Department of Clinical Medicine / Faculty of Medical Sciences

Books

Okumura T, Ohuchida K, Nakamura M, An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding, Humana Press, 10.1007/978-1-4939-8879-2_11, Methods Mol Biol,1882:135-141, 2018.04, Abstract
Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue..

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