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Naoki Iwamori Last modified date:2023.11.27

Associate Professor / Agricultural Bioresource Sciences
Department of Bioresource Sciences
Faculty of Agriculture

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Stem cell biology, Epigenetics, Reproductive biology
Total Priod of education and research career in the foreign country
Outline Activities
My research interest is epigenetic regulation, evolution, and diversity of development and differentiation of stem cells as well as germ cells. Now I am focusing on epigenetic regulation of spermatogonial, neural, and pluripotent stem cells as well as gametogenesis.
Research Interests
  • Validation and dissection of stem cell ability in spiny mouse (Acomys cahirinus).
    keyword : spiny mouse, Acomys cahirinus, iPS cell, pluripotent stem cell, tissue stem cell, self-renewal
  • Generation of induced pluripotent stem cells and in vitro derivation of germ cells from non-rodent mammals to apply for rare animals
    keyword : pluripotent stem cells, iPS cells, in vitro derivation of germ cells, rare animals
  • The role of histone demethylases on sex chromosome in the sex determination.
    keyword : sex determination, germ cell, histone demethylase, sex chromosome, gender difference, brain
  • The study of epigenetic regulation during neural differentiation
    keyword : epigenetics, neural stem cell, neural differentiation, histone demethylase
  • Epigenetic regulation of germ cell development and differentiation
    ・The role of histone demethylases in the regulation of spermatogonial stem cells
    ・Regulatory mechanisms of histone acetylation during spermatogenesis
    keyword : Epigenetics, Spermatogonial stem cells, Spermatogenesis, Oogenesis, Histone demethylase, Acetylation of histones
Academic Activities
1. N. IWAMORI, Regulation of spermatogonial stem cell compartment in the mouse testis, Fukuoka Igaku Zassh, 2014.01.
1. Sultana Tasrin, Iwamori Tokuko, Iwamori Naoki, TSNAXIP1 is required for sperm head formation and male fertility., Reproductive Medicine and Biology, 10.1002/rmb2.12520, 22, 1, e12520, 2023.06.
2. Tominaga Kaoru、Sakashita Eiji、Kasashima Katsumi、Kuroiwa Kenji、Nagao Yasumitsu、Iwamori Naoki、Endo Hitoshi, Tip60/KAT5 Histone Acetyltransferase Is Required for Maintenance and Neurogenesis of Embryonic Neural Stem Cells., International Journal of Molecular Sciences, 10.3390/ijms24032113, 24, 3, 2113, 2023.01.
3. Wang Y, Iwamori T, Kaneko T, Iida H, Iwamori N., Comparative distributions of RSBN1 and methylated histone H4 Lysine 20 in the mouse spermatogenesis, PLOS One, 10.1371/journal.pone.0253897, 16, 6, e0253897, 2021.06, During spermatogenesis, nuclear architecture of male germ cells is dynamically changed and epigenetic modifications, in particular methylation of histones, highly contribute to its regulation as well as differentiation of male germ cells. Although several methyltransferases and demethylases for histone H3 are involved in the regulation of spermatogenesis, roles of either histone H4 lysine 20 (H4K20) methyltransferases or H4K20 demethylases during spermatogenesis still remain to be elucidated. Recently, RSBN1 which is a testis-specific gene expressed in round spermatids was identified as a demethylase for dimethyl H4K20. In this study, therefore, we confirm the demethylase function of RSBN1 and compare distributions between RSBN1 and methylated H4K20 in the seminiferous tubules. Unlike previous report, expression analyses for RSBN1 reveal that RSBN1 is not a testis-specific gene and is expressed not only in round spermatids but also in elongated spermatids. In addition, RSBN1 can demethylate not only dimethyl H4K20 but also trimethyl H4K20 and could convert both dimethyl H4K20 and trimethyl H4K20 into monomethyl H4K20. When distribution pattern of RSBN1 in the seminiferous tubule is compared to that of methylated H4K20, both dimethyl H4K20 and trimethyl H4K20 but not monomethyl H4K20 are disappeared from RSBN1 positive germ cells, suggesting that testis-specific distribution patterns of methylated H4K20 might be constructed by RSBN1. Thus, novel expression and function of RSBN1 could be useful to comprehend epigenetic regulation during spermatogenesis..
4. Naoki IWAMORI, Kaoru Tominaga, Sato Tetsuya, Kevin Riehle, Tokuko Iwamori, Yasuyuki Ohkawa, Cristian Coarfa, Etsuro Ono, Martin M. Matzuk, MRG15 is required for pre-mRNA splicing and spermatogenesis, PNAS, 10.1073/pnas.1611995113, 113, 37, E5408-E5415, 2016.09, Splicing can be epigenetically regulated and involved in cellular differentiation in somatic cells, but the interplay of epigenetic factors and the splicing machinery during spermatogenesis re- mains unclear. To study these interactions in vivo, we generated a germline deletion of MORF-related gene on chromosome 15 (MRG15), a multifunctional chromatin organizer that binds to methylated histone H3 lysine 36 (H3K36) in introns of transcriptionally active genes and has been implicated in regulation of histone acetyla- tion, homology-directed DNA repair, and alternative splicing in somatic cells. Conditional KO (cKO) males lacking MRG15 in the germline are sterile secondary to spermatogenic arrest at the round spermatid stage. There were no significant alterations in meiotic division and histone acetylation. Specific mRNA sequences disappeared from 66 germ cell-expressed genes in the absence of MRG15, and specific intronic sequences were retained in mRNAs of 4 genes in the MRG15 cKO testes. In particular, introns were retained in mRNAs encoding the transition proteins that replace histones during sperm chromatin condensation. In round spermatids, MRG15 colocalizes with splicing factors PTBP1 and PTBP2 at H3K36me3 sites between the exons and single intron of transition nuclear protein 2 (Tnp2). Thus, our results reveal that MRG15 is essential for pre- mRNA splicing during spermatogenesis and that epigenetic regula- tion of pre-mRNA splicing by histone modification could be useful to understand not only spermatogenesis but also, epigenetic disor- ders underlying male infertile patients..
5. Dae Hwi Park, Sung Jun Hong, Ryan D. Salinas, Siyuan John Liu, Shawn W. Sun, Jacopo Sgualdino, Giuseppe Testa, Martin M. Matzuk, N. IWAMORI, Daniel A. Lim, Activation of Neuronal Gene Expression by the JMJD3 Demethylase Is Required for Postnatal and Adult Brain Neurogenesis, CELL REPORTS, 10.1016/j.celrep.2014.07.060, 8, 5, 1290-1299, 2014.09.
6. Naoki Iwamori, Tokuko Iwamori, Martin M. Matzuk, H3K27 demethylase, JMJD3, regulates fragmentation of spermatogonial cysts., PLoS ONE, 10.1371/journal.pone.0072689, 8, 8, e72689, 2013.08.
7. Iwamori N, Iwamori T, Matzuk MM, Characterization of spermatogonial stem cells lacking intercellular bridges and genetic replacement of a mutation in spermatogonial stem cells., PLoS One, 7, 6, e38914, 2012.06.
8. Iwamori N, Zhao M, Meistrich ML, Matzuk MM, The testis-enriched histone demethylase, JMJD2D, regulates methylation of histone H3 lysine 9 during spermatogenesis but is dispensable for fertility. , Biol. Reprod, 84, 6, 1225-1234, 2011.06.
1. Tasrin Sultana、Tokuko Iwamori、Naoki Iwamori, Localization and functional analysis of predominant testis expressed protein TSNAXIP1, 第115回日本繁殖生物学会大会, 2022.09.
2. Tasrin Sultana、岩森督子、岩森巨樹, TEX14部分ペプチドの抗腫瘍活性, 第93回日本動物学会早稲田大会, 2022.09.
3. Tasrin Sultana, Tokuko Iwamori, Naoki Iwamori, TEX14部分ペプチドの抗腫瘍活性, 第78回 九州・沖縄生殖医学会, 2022.04.
4. Sakurako Shima, Tokuko Iwamori, Hiroshi Iida, Naoki Iwamori, Regulation of spermatogonial stem cells by H3K27 demethylases., The 52th SSR annual meeting, 2019.07.
5. Naoki Iwamori, Sakurako Shima, Tokuko Iwamori, Hiroshi Iida, The role of H3K27 demethylases in the regulation of spermatogonial stem cells, ISSCR 2019, 2019.06.
6. Sakurako Shima、Hiroshi Iida、Naoki Iwamori, Regulation of spermatogonial stem cells by H3K27 demethylases., ISSCR 2018, 2018.06.
7. Naoki Iwamori, Sakurako Shima, Tokuko Iwamori, Hiroshi Iida, The role of H3K27 demethylases in the regulation of spermatogonial stem cells, 第16回幹細胞シンポジウム, 2018.06, 精子形成の起源となる精子幹細胞の制御機構を解析し、ヒストンH3リジン27(H3K27)の脱メチル化酵素が幹細胞老化に関与することを見出した。生殖細胞特異的にH3K27脱メチル化酵素JMJD3を欠損した雄マウスは長期にわたり良好な繁殖能力を示し、実際に、精巣内の精子幹細胞数増加、脱分化的幹細胞供給の増加が見られた。精巣以外の組織を含めたJMDJ3による幹細胞制御機構を調べたところ、神経幹細胞の分化に寄与することが明らかとなったほか、飢餓ストレスを与えた際に肝臓においてミトコンドリア脂肪酸酸化経路を活性化することが示された。精巣においてもエネルギー代謝経路、オートファジー経路とJMJD3の関連が幹細胞老化に関わることが示され、幹細胞制御に応用できる可能性が示唆された。.
8. Iwamori N, Iwamori T, Shima S, Iida H, The role of JMJD3 in the regulation of intercellular bridges during the fragmentation of spermatogonial cysts., The 4th WCRB, 2017.09.
9. Iwamori N, Iwamori T, Iida H, The role of JMJD3 in the regulation of spermatogonial stem cells., ISSCR 2017, 2017.06.
10. N. IWAMORI, The role of H3K27 demethylase, JMJD3, in the regulation of spermatogonial stem cells, The 3rd SKLRB Symposium on Reproductive Biology, 2014.10.
Membership in Academic Society
  • Japan Society for Reproductive Medicine
  • Stem Cell Research Symposium
  • The Zoological Society of Japan
  • Japanese Society for Epigenetics
  • The Society of Reproduction and Development
  • The Molecular Biology Society of Japan
  • The society for study of reproduction
  • International society for stem cells research