Kyushu University Academic Staff Educational and Research Activities Database
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Shibata Toshio Last modified date:2023.11.27

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Philosophy
Country of degree conferring institution (Overseas)
Field of Specialization
Biochemistry, Innate immunity, Protein science
Total Priod of education and research career in the foreign country
Research Interests
  • Unconventional protein secretion pathway mediated by fatty acylation
    keyword : Myristoylation, Palmitoylation, Exosomes
  • Transglutaminase-catalyzed Crosslinking Suppresses Innate Immune Signaling in the Drosophila Gut
    keyword : Transglutaminase, Drosophila, IMD pathway
Academic Activities
1. Shibata Toshio, Shun-ichiro Kawabata, Transglutaminases-Multiple Functional Modifiers and Targets for New Drug Discovery, Springer Japan, 2015.12, The existing knowledge of invertebrate transglutaminases with an emphasis on the importance of various physiological properties in innate immune reactions..
1. Pluripotency and a secretion mechanism of Drosophila transglutaminase
Transglutaminase (TG) catalyses the formation of an isopeptide bond between glutamine and lysine residues and amine incorporation into specific glutamine residues. TG is conserved in all metazoans and functions both intracellularly and extracellularly. Here we review the existing knowledge of Drosophila TG with an emphasis on its pluripotency: Drosophila TG (i) plays a key role in cuticular morphogenesis, haemolymph coagulation, and entrapment against invading pathogens, (ii) suppresses the immune deficiency pathway to enable immune tolerance against commensal bacteria through the incorporation of polyamines into the nuclear factor-iB-like transcription factor Relish as well as through the protein-protein cross-linking of Relish, (iii) forms a physical matrix in the gut through cross-linking of chitin-binding proteins and (iv) is involved in the maintenance of homeostasis in microbiota in the gut. Moreover, we review the evidence that TG-A, one of alternative splicing-derived isoforms of Drosophila TG, is secreted through an endoplasmic reticulum/Golgi-independent pathway involving exosomes and fatty acylations..
1. Toshio Shibata, Jinki Hadano, Daichi Kawasaki, Xiaoqing, Shun-ichiro Kawabata, Drosophila TG-A transglutaminase is secreted via an unconventional Golgi-independent mechanism involving exosomes and two types of fatty acylations, The Journal of Biological Chemistry, 10.1074/jbc.M117.779710, 292, 25, 10723-10734, 2017.06.
2. Toshio Shibata, Kouki Maki, Jinki Hadano, Takumi Fujikawa, Kazuki Kitazaki, Takumi Koshiba, Shun-ichiro Kawabata, Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins, PLOS Pathogens, 10.1371/journal.ppat.1005244, 11, 10, e1005244, e1005244, 2015.10.
3. Toshio Shibata, Sanae Sekihara, Takumi Fujikawa, Ryuta Miyaji, Kouki Maki, Takeshi Ishihara, Takumi Koshiba, Shun-ichiro Kawabata, Transglutaminase-Catalyzed Protein-Protein Cross-Linking Suppresses the Activity of the NF-κB–Like Transcription Factor Relish, Science Signaling, 10.1126/scisignal.2003970, 6, 285, ra61, 2013.07.
1. 柴田 俊生, Transglutaminase-Catalyzed Protein-Protein Crosslinking Maintains the Gut Epithelial Immunity in Drosophila, The 55th Annual Drosophila Research Conference, 2014.03.