Kyushu University Academic Staff Educational and Research Activities Database
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Hisako TAKIGAWA-IMAMURA Last modified date:2021.07.07

Assistant Professor / Bioregulation
Department of Basic Medicine
Faculty of Medical Sciences

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Philosophy
Country of degree conferring institution (Overseas)
Yes Doctor
Field of Specialization
Mathematical Biology, Developmental Biology, Biophysics
Total Priod of education and research career in the foreign country
Outline Activities
Theoretical investigation in developmental biology.
Research Interests
  • Mathematical modeling for the branching formation by cell migration of endothelial cells
    keyword : mathematical model, branching pattern, angiogenesis
  • Computational model of the leaf shape change owing to epidermal deformation
    keyword : Arabidopsis, Ric-1, computational model, epidermal cell
  • Mechanism underlying dynamic scaling properties observed in the contour of spreading epithelial monolayer
    keyword : epithelial cell, collective cell migration, self-affine fractal
  • Phosphatidylinositol 3-Phosphate 5-kinase, FAB1 and Rho-of-Plant 10coordinately mediate root hair shank hardening in Arabidopsis
    keyword : Arabidopsis, Mathematical model, root hair
  • Mechanical modeling for intestinal villi formation
    keyword : intestine, villi, mathematical model, buckling
  • Modeling of the cell shape change during lung morphogenesis
    keyword : lung, morphogenesis, Eisuke transgenic mouse, ERK, FGF10
  • Numerical simulation for the uplift pattern of leaf epidermal cells
    keyword : Arabidopsis, Mathematical model, pavement cell, guard cell
  • Mathematical modeling of the jigsaw-like epidermis pattern of dicot plants
    keyword : mathematical mode, epidermis, dicotyledon
  • Building vascularized tissues in vitro
    keyword : organ culture, vascular endothelial cells, angiogenesis, lung
  • Imaging FGF-signaling in lung morphogenesis
    keyword : lung, morphogenesis, Eisuke transgenic mouse, ERK, FGF10
  • Application of microfluidics for the imaging of angiogenesis in tooth germ
    keyword : microfluidics, angiogenesis, tooth germ
  • Microfluidics-generated biomolecules gradient for bioimaging
    keyword : microfluidics, morphogen, bioimaging
  • The role of VEGF in cell migration during angiogenesis
    keyword : angiogenesis, cell migration, angiogenesis
  • Mathematical model of cap stage formation of tooth germ

    keyword : tooth germ, mathematical model, buckling
Academic Activities
1. Tomoko Hirano, Hiroki Konno, Seiji Takeda, Liam Dolan, Mariko Kato, Takashi Aoyama, Takumi Higaki, Hisako Takigawa-Imamura, Masa H Sato, PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis., Nature plants, 10.1038/s41477-018-0277-8, 4, 11, 888-897, 2018.11, Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the mechanism by which root hairs harden the shank. Here we show that phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2), the enzymatic product of FORMATION OF APLOID AND BINUCLEATE CELLS 1 (FAB1), is involved in the hardening of the shank in root hairs in Arabidopsis. FAB1 and PtdIns(3,5)P2 localize to the plasma membrane along the shank of growing root hairs. By contrast, phosphatidylinositol 4-phosphate 5-kinase 3 (PIP5K3) and PtdIns(4,5)P2 localize to the apex of the root hair where they are required for tip growth. Reduction of FAB1 function results in the formation of wavy root hairs while those of the wild type are straight. The localization of FAB1 in the plasma membrane of the root hair shank requires the activity of Rho-related GTPases from plants 10 (ROP10) and localization of ROP10 requires FAB1 activity. Computational modelling of root hair morphogenesis successfully reproduces the wavy root hair phenotype. Taken together, these data demonstrate that root hair shank hardening requires PtdIns(3,5)P2/ROP10 signalling..
2. Katsumi Fumoto, Hisako Takigawa-Imamura, Kenta Sumiyama, Tomoyuki Kaneiwa, Akira Kikuchi, Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition., Development, 10.1242/dev.141325, 144, 1, 151-162, 2017.01, In lung development, the apically constricted columnar epithelium forms numerous buds during the pseudoglandular stage. Subsequently, these epithelial cells change shape into the flat or cuboidal pneumocytes that form the air sacs during the canalicular and saccular (canalicular-saccular) stages, yet the impact of cell shape on tissue morphogenesis remains unclear. Here, we show that the expression of Wnt components is decreased in the canalicular-saccular stages, and that genetically constitutive activation of Wnt signaling impairs air sac formation by inducing apical constriction in the epithelium as seen in the pseudoglandular stage. Organ culture models also demonstrate that Wnt signaling induces apical constriction through apical actomyosin cytoskeletal organization. Mathematical modeling reveals that apical constriction induces bud formation and that loss of apical constriction is required for the formation of an air sac-like structure. We identify MAP/microtubule affinity-regulating kinase 1 (Mark1) as a downstream molecule of Wnt signaling and show that it is required for apical cytoskeletal organization and bud formation. These results suggest that Wnt signaling is required for bud formation by inducing apical constriction during the pseudoglandular stage, whereas loss of Wnt signaling is necessary for air sac formation in the canalicular-saccular stages..
3. Hisako Takigawa-Imamura, Ritsuko Morita, Takafumi Iwaki, Takashi Tsuji, Kenichi Yoshikawa, Tooth germ invagination from cell-cell interaction: Working hypothesis on mechanical instability., Journal of theoretical biology, 10.1016/j.jtbi.2015.07.006, 382, 284-91, 2015.10, In the early stage of tooth germ development, the bud of the dental epithelium is invaginated by the underlying mesenchyme, resulting in the formation of a cap-like folded shape. This bud-to-cap transition plays a critical role in determining the steric design of the tooth. The epithelial-mesenchymal interaction within a tooth germ is essential for mediating the bud-to-cap transition. Here, we present a theoretical model to describe the autonomous process of the morphological transition, in which we introduce mechanical interactions among cells. Based on our observations, we assumed that peripheral cells of the dental epithelium bound tightly to each other to form an elastic sheet, and mesenchymal cells that covered the tooth germ would restrict its growth. By considering the time-dependent growth of cells, we were able to numerically show that the epithelium within the tooth germ buckled spontaneously, which is reminiscent of the cap-stage form. The difference in growth rates between the peripheral and interior parts of the dental epithelium, together with the steric size of the tooth germ, were determining factors for the number of invaginations. Our theoretical results provide a new hypothesis to explain the histological features of the tooth germ..
1. 今村寿子, Observation of FGF response in lung epithelium and modeling for
branching morphogenesis, 新学術領域研究「上皮管腔組織形成」第2回国際シンポジウム, 2015.08, The differences in cellular behavior underlying morphogenesis are governed by signaling interactions in the growing tissue. In lung branching morphogenesis, for instance, the high sensitivity of cells to the distribution of diffusive signals within the developing tissue is considered to be the principle mechanism guiding shape change. Here I investigated the response and sensitivity of lung epithelium to FGF10 that mediates epithelial branching to realize the tissue-specific shape. I demonstrated that uptake of FGF10 by epithelial explants of the pseudoglandular stage lung in Matrigel was sensitive over a wide range of FGF10 concentrations in the gel. It was also indicated that MAP kinase activity downstream of FGF10 was affected by the epithelial explant size and shape as well as the FGF10 concentration. These cellular responses of lung epithelium to FGF10 were higher in E13 than E14. To assess how these cellular responses result in shape formation of the lung epithelium, I constructed a framework employing a mathematical model in which an epithelial tip splits depending on the proliferative and chemotactic activities. Experimental results on lung epithelium were incorporated into the model and how the ordered structure of lung emerges will be discussed..
Membership in Academic Society
  • The Biophysical Society of Japan
  • Molecular Biology Society of Japan
  • Japanese Society of Developmental Biologists
  • Japanese Society for Mathematical Biology
Educational Activities
Histology and Anatomy