Language：English   Publishing type：Research paper (scientific journal)  

Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering.

DOI： 10.1016/j.jbiosc.2013.05.020

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

Growth factors (GFs) are indispensable in regenerative medicine because of their high effectiveness. However, as GFs degenerate easily, the development of a suitable carrier with improved stability for GFs is necessary. In this study, we developed a gel-in-oil (G/O) emulsion technology for the transdermal delivery of growth factors. Nanogel particles prepared with heparin-immobilized gelatin that can bind growth factors were dispersed in isopropyl myristate. The particle size of the G/O emulsion could be controlled by changing the surfactant concentration, volume ratio of the water phase to the oil phase, and gelatin concentration. In vitro skin penetration studies showed better penetration through the stratum corneum of fluorescent proteins containing G/O emulsions than of the aqueous solution of GF. Similarly, an in vivo study showed an angiogenesis-inducing effect after transdermal application of GF-immobilized G/O emulsion. Angiogenesis in mice was confirmed owing to both an increased blood vessel network and higher hemoglobin content in the blood. Therefore, the G/O emulsion could be a promising carrier for GFs with better stability and can effectively deliver GFs at the target site.

DOI： 10.1016/j.jbiosc.2021.03.015

Language：Japanese  

Language：Others   Publishing type：Research paper (scientific journal)  

© 2020 The Japanese Society for Regenerative Medicine Introduction: Cells have various applications in biomedical research. Cryopreservation is a cell-preservation technique that provides cells for such applications. After cryopreservation, sensitive cells, such as primary hepatocytes, suffer from low viability due to the physical damage caused by ice crystals, highlighting the need for better methods of cryopreservation to improve cell viability. Given the importance of effectively suppressing ice crystal formation to protect cellular structure, trehalose has attracted attention as cryoprotectant based on its ability to inhibit ice crystal formation; however, trehalose induces osmotic stress. Therefore, to establish a cell-cryopreservation technique, it is necessary to provide an optimal balance between the protective and damaging effects of trehalose. Methods: In this study, we evaluated the effects of osmotic stress and ice crystal formation on the viability and function of primary rat hepatocytes at wide range of trehalose concentration. Results: There was no osmotic stress at very low concentrations (2.6 μM) of trehalose, and 2.6 μM trehalose drives the formation of finer ice crystals, which are less damaging to the cell membrane. Furthermore, we found that the number of viable hepatocytes after cryopreservation were 70% higher under the 2.6 μM trehalose-supplemented conditions than under the dimethyl sulfoxide-supplemented conditions. Moreover, non-cryopreserved cells and cells cryopreserved with trehalose showed comparable intracellular dehydrogenase activity. Conclusions: We showed that trehalose at very low concentrations (2.6 μM) improved dramatically viability and liver function of hepatocyte after cryopreservation.

DOI： 10.1016/j.reth.2020.08.003

Language：English   Publishing type：Research paper (scientific journal)  

© 2019 Elsevier B.V. We developed a new oil-based delivery system for transdermal protein delivery, a gel-in-oil (G/O) nanosuspension, where gelatin-based hydrogel was coated with hydrophobic surfactants. The high entrapment efficiency of a model protein, phycocyanin (PC), into nano-sized gelatin hydrogel particles was achieved. Spectroscopic evaluation of PC suggested that the G/O nanosuspension could retain the functional form of PC in isopropyl myristate. In vitro skin permeation studies showed that the G/O nanosuspension facilitated the delivery of PC through the stratum corneum of Yucatan micropig skin.

DOI： 10.1016/j.ijpharm.2019.118495

Language：Others   Publishing type：Research paper (scientific journal)  

Aims: The main aim of our study was to examine the concentration of surfactant that can cause significant disruption of the resulting decellularized liver structure. Furthermore, it is our goal to determine the suitable solvent that can boost the potential of each surfactant.
Methodology: The porcine liver discs of 8-mm diameter and 2-mm thickness were prepared. These were soaked in aqueous solution of either sodium dodecyl sulfate (SDS) or Triton X-100 (TX), and placed on a rotational shaking machine (100 rpm).
Results: TX was unable to completely remove the cellular components under any of our experimental conditions. The salt concentration did not affect the decellularization in TX. The pH buffer, however, was found to affect the decellularization. Also, in the solvent study, the conditions under which SDS effectively exerted power were not the salt concentration and pH, but the condition that was close to water. We also confirmed that the shrinkage of tissue occurred when decellularization with 0.1% SDS in CMF-PBS. However, 0.1% SDS in distilled water didn't cause the deformation of tissue. This is considered to be due to the low salt concentration of solvent.
Conclusion: This work establishes the concentration range of the surfactant that causes the collapse of the cellular structure during decellularization. In addition, the solvent suitable for each surfactant has also been established.

DOI： 10.9734/ajrb/2019/v5i130079

Language：English   Publishing type：Research paper (scientific journal)  

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Hydrogels and their medical applications in tissue engineering have been widely studied due to their three-dimensional network structure, biocompatibility, and cell adhesion. However, the development of an artificial bile duct to replace the recipient’s tissue is still desired. Some challenges remain in the tissue engineering field, such as infection due to residual artifacts. In other words, at present, there are no established technologies for bile duct reconstruction as strength and biocompatibility problems. Therefore, this study investigated hydrogel as an artificial bile duct base material that can replace tissue without any risk of infectious diseases. First, an antibacterial agent (ABA), Finibax (an ABA used for the clinical treatment of biliary tract infection), was immobilized in gelatin using a crosslinking agent, and the antibacterial properties of the gel and its sustainability were tested. Furthermore, the immobilized amount and the improvement of the proliferation of the human umbilical vein endothelial cells (HUVECs) were cultured as the ABA-Gelatin hydrogel was introduced to prepare a 3D scaffold. Finally, we performed hematoxylin and eosin (H&E) staining after subcutaneous implantation in the rat. Overall, the ABA-Gelatin hydrogel was found to be viable for use in hydrogel applications for tissue engineering due to its good bactericidal ability, cell adhesion, and proliferation, as well as having no cytotoxicity to cells.

DOI： 10.3390/gels5020032

Language：English   Publishing type：Research paper (scientific journal)  

© 2018 Acta Materialia Inc. Fabrication of functional scaffolds is one of the main goals of tissue engineering, particularly scaffolds mimicking the native extracellular matrix (ECM) and stimulating a microenvironment that supports cell growth and function. In this study, a scaffold was developed using liver-derived ECM (L-ECM), gelatin, and polycaprolactone (PCL) by conventional electrospinning. This study aimed to improve the gelatin-PCL nanofiber blend by incorporating L-ECM. The morphology of the nanofibrous scaffold was investigated by scanning electron microscopy. Characterizations such as Fourier transform infrared spectroscopy (FTIR), tensile strength test, and water contact angle analysis revealed the favorable characteristics of the scaffold. Particularly, the FTIR spectra confirmed the presence of L-ECM, gelatin, and PCL, even after crosslinking treatment, while tensile strength analysis revealed suitable mechanical properties and water contact angle measurement showed the improved hydrophilic characteristics of the scaffold. The biocompatibility of the composite nanofibers was evaluated by in vitro culture of primary hepatocytes. Phase-contrast microscope images verified that the L-ECM nanofibers improved hepatocyte adhesion and facilitated the formation of a tissue-like structure. Moreover, high liver-specific functions were obtained in L-ECM nanofibers compared to gelatin/PCL nanofibers. Thus, L-ECM nanofibers can be used in tissue engineering, particularly in liver regeneration.

DOI： 10.1016/j.mtla.2018.11.014

Language：Others  

DOI： 10.1002/9781119296034.ch3

Language：English   Publishing type：Research paper (scientific journal)  

The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 degrees C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified.

DOI： 10.3390/gels4020039

Language：English   Publishing type：Research paper (scientific journal)  

Two-dimensional monolayer culture is the most popular cell culture method. However, the cells may not respond as they do in vivo because the culture conditions are different from in vivo conditions. However, hydrogel-embedding culture, which cultures cells in a biocompatible culture substrate, can produce in vivo-like cell responses, but in situ evaluation of cells in a gel is difficult. In this study, we realized an in vivo-like environment in vitro to produce cell responses similar to those in vivo and established an in situ evaluation system for hydrogel-embedded cell responses. The extracellular matrix (ECM)-modeled gel consisted of collagen and heparin (Hep-col) to mimic an in vivo-like environment. The Hep-col gel could immobilize growth factors, which is important for ECM functions. Neural stem/progenitor cells cultured in the Hep-col gel grew and differentiated more actively than in collagen, indicating an in vivo-like environment in the Hep-col gel. Second, a thin-layered gel culture system was developed to realize in situ evaluation of the gel-embedded cells. Cells in a 200-μm-thick gel could be evaluated clearly by a phase-contrast microscope and immunofluorescence staining through reduced optical and diffusional effects. Finally, we found that the neural cells cultured in this system had synaptic connections and neuronal action potentials by immunofluorescence staining and Ca²⁺ imaging. In conclusion, this culture method may be a valuable evaluation system for neurotoxicity testing.

DOI： 10.1016/j.jbiosc.2017.04.018

Language：English   Publishing type：Research paper (scientific journal)  

Conventionally, some bioartificial liver devices are used with separate plasmapheresis unit to separate out plasma from whole blood and adsorbent column to detoxify plasma before it passes through a hepatocytes-laden bioreactor. We aim to develop a hybrid bioreactor that integrates the separate modules in one compact design improving the efficacy of the cryogel based bioreactor as a bioartificial liver support. A plasma separation membrane and an activated carbon cloth are placed over a HepG2-loaded cryogel scaffold in a three-chambered bioreactor design. This bioreactor is consequently connected extracorporeally to a rat model of acute liver failure for 3 h and major biochemical parameters studied. Bilirubin and aspartate transaminase showed a percentage decrease of 20-60% in the integrated bioreactor as opposed to 5-15% in the conventional setup. Urea and ammonia levels which showed negligible change in the conventional setup increase (40%) and decrease (18%), respectively in the integrated system. Also, an overall increase of 5% in human albumin in rat plasma indicated bioreactor functionality in terms of synthetic functions. These results were corroborated by offline evaluation of patient plasma. Hence, integrating the plasmapheresis and adsorbent units with the bioreactor module in one compact design improves the efficacy of the bioartificial liver device.

DOI： 10.1038/srep40323

Language：Others  

© Springer Nature Singapore Pte Ltd. 2017. Tissue Engineering consists of cells, a scaffold and cytokines. Decellularization represents the removal of cells from tissues or organs. Recently, decellularized tissue has been investigated as a scaffold for tissue engineering, termed decellularized tissue engineering. Importantly, the decellularized organ retains its original structure, which is then used as a template for organ construction. The decellularized organ also retains the tissue-specific extracellular matrix. Therefore, decellularized tissue can be used as a matrix to provide a suitable microenvironment for inoculated cells. Based on these concepts, the reconstruction of tissues/organs with decellularized tissue/organ has been attempted using decellularized tissue engineering. In this chapter, we introduce the typical methods used, history and attainment level for the reconstruction of specific tissues/organs. First, the different decellularized techniques and characteristics are introduced. Then, the commonly used analysis methods and cautionary points during decellularization and reconstruction with decellularized tissues/organs are explained. Next, the specific methods and characteristics of decellularized tissue engineering for specific tissues/organs are introduced. In these sections, the current conditions, problems and future work are explained. Finally, we conclude with a summary of this chapter.

DOI： 10.1007/978-981-10-3328-5_5

Language：English   Publishing type：Research paper (scientific journal)  

DOI： DOI:10.14326/abe.5.105

Other Link： https://www.jstage.jst.go.jp/article/abe/5/0/5_5_105/_article

Language：English   Publishing type：Research paper (scientific journal)  

Cell transplantation is a potential alternative for orthotopic liver transplantation because of the chronic donor shortage. Functional liver tissue is needed for cell transplantations. However, large functional liver tissue is difficult to construct because of the high oxygen consumption of hepatocytes. In our previous study, we developed a novel method to generate hybrid organoids. In this study, we used fetal liver cells (FLCs) to construct a hybrid organoid. Nucleus numbers, angiogenesis, and albumin production were measured in transplanted samples. Higher cell viability and larger liver tissue was found in FLC-containing samples than in hepatocyte-containing samples. Furthermore, the therapeutic efficiency of FLC-containing samples was evaluated by transplantation into Nagase analbuminemia rats. As a result, an increase in albumin concentration was found in rat blood. In summary, transplantation of a FLC-containing hybrid organoid is a potential approach for cell transplantation.

DOI： 10.1016/j.jbiosc.2015.11.006

Language：English   Publishing type：Research paper (scientific journal)  

Hepatocyte transplantation is a potential therapy for treating various liver diseases. However, oxygen shortage leading to loss of hepatocyte function becomes a limitation following hepatocyte transplantation. To overcome this problem, we developed a hybrid organoid, consisting of growth factor (GF)-immobilizable gel particles combined with hepatocytes. Benefits of the hybrid organoid were evaluated in three groups: (i) hybrid organoid consisting of cells and GF-immobilizable gel particles (HG-C); (ii) hybrid organoid consisting of cells and gel particles (G-C); and (iii) cells suspended in collagen (C-C). We found liver-specific functions of HG-C were maintained longer than in the other conditions during in vitro culture. Furthermore, after transplantation, HG-C was effective in maintaining viability of transplanted hepatocytes and promoting angiogenesis around the hepatocytes. In summary, transplantation of HG-C is a potential method for future liver tissue engineering.

DOI： 10.1016/j.jbiosc.2015.01.004

Language：English   Publishing type：Research paper (scientific journal)  

The growth of transplanted hepatocytes is required for the construction of tissue-engineered liver. In this study, cell-embedded hydrogel-filled polyurethane foam plates were subcutaneously transplanted into rat. A liver tissue-like structure was formed by transplanted fetal liver cells in 70% partial hepatectomy treated rat.

DOI： 10.1016/j.jbiosc.2012.11.015

Language：English   Publishing type：Research paper (scientific journal)  

Growth factor (GF)-immobilizable materials were developed as a practical hepatocyte transplantation method for reconstructing a tissue-like structure in liver tissue engineering. Two GF-immobilizable scaffolds, namely single hepatocyte-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam, and hepatocyte spheroid-embedded, heparin-immobilized, collagen-gel-filled polyurethane foam were developed by covalently incorporating heparin into collagen gel, using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/. N-hydroxysuccinimide for hepatocyte transplantation. Seventy percent partial hepatectomy (PH) was performed at the same time after hepatocyte transplantation. Angiogenesis efficiency and viability of transplanted cells are discussed in terms of normalized hemoglobin content, nuclear density and histological observations after transplantation. In summary, the normalized hemoglobin content and viability of transplanted cells were higher in GF-immobilized scaffolds with PH pretreatment than in the other scaffolds with/without PH pretreatment. These materials have the potential for in vivo hepatocyte transplantation, as GFs released from remnant liver were easily incorporated into the heparin-immobilized collagen gel system. These GF-heparin complexes may promote the survival of embedded cells. Furthermore, the transplantation of spheroids promoted increased angiogenesis compared with hepatocytes, and resulted in sufficient vascularization for cell survival.

DOI： 10.1016/j.bej.2012.09.007

Language：English   Publishing type：Research paper (scientific journal)  

The construction of a functional liver-tissue equivalent using tissue engineering is a very important goal because the liver is a central organ in the body. However, the construction of functional organ-scale liver tissue is impossible because it requires a high-density blood vessel network. In this study, we focused on decellularization technology to solve this problem. Decellularized liver tissue with a fine vascular tree network template was obtained using Triton X-100. The distance between each vascular structure was less than 1 mm. Endothelialization of the blood vessel network with human umbilical vein endothelial cells (HUVECs) was successfully performed without any leakage of HUVECs to the outside of the vessel structure. Furthermore, hepatocytes/spheroids could be located around the blood vessel structure. This study indicates that decellularized liver tissue is a potential scaffold for creating a practical liver tissue using tissue engineering technology.

DOI： 10.1016/j.jbiosc.2012.05.022

Event date： 2015.3

Language：Japanese  

Venue：横浜   Country：Japan  

Event date： 2014.11

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：福岡   Country：Japan  

Language：English  

Venue：熊本   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：岐阜   Country：Japan  

Venue：京都   Country：Japan  

Venue：京都   Country：Japan  

Language：Japanese  

Venue：熊本   Country：Japan  

Event date： 2023.3

Language：Japanese  

Venue：国立京都国際会館   Country：Japan  

Event date： 2023.3

Language：Japanese  

Venue：国立京都国際会館   Country：Japan  

Event date： 2023.3

Language：Japanese  

Venue：国立京都国際会館   Country：Japan  

Event date： 2023.3

Language：Japanese  

Venue：東京農工大学 小金井キャンパス   Country：Japan  

Event date： 2022.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2022.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2022.3

Language：Japanese  

Event date： 2022.3

Language：English  

Event date： 2021.11

Language：Japanese  

Event date： 2020.5

Language：Japanese  

Event date： 2020.5

Language：Japanese  

Event date： 2020.3

Language：Japanese  

Event date： 2018.9

Language：English  

Venue：国立京都国際会館   Country：Japan  

Event date： 2018.9

Language：English  

Venue：国立京都国際会館   Country：Japan  

Event date： 2018.9

Language：English  

Venue：国立京都国際会館   Country：Japan  

Event date： 2018.9

Language：English  

Venue：国立京都国際会館   Country：Japan  

Event date： 2018.9

Language：English  

Venue：国立京都国際会館   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2018.3

Language：Japanese  

Venue：関西大学　千里山キャンパス   Country：Japan  

Event date： 2018.3

Language：Japanese  

Venue：九州工業大学　飯塚キャンパス   Country：Japan  

Event date： 2018.3

Language：Japanese  

Venue：九州工業大学　飯塚キャンパス   Country：Japan  

Event date： 2018.1

Language：English  

Venue：OIST   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.10

Language：Japanese  

Venue：九州大学西新プラザ   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：九州大学   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2017.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2017.3

Language：Japanese  

Venue：九州大学　伊都キャンパス   Country：Japan  

Event date： 2017.3

Language：Japanese  

Venue：九州大学　伊都キャンパス   Country：Japan  

Event date： 2017.3

Language：Japanese  

Venue：九州大学　伊都キャンパス   Country：Japan  

Event date： 2016.12

Language：English  

Country：Thailand  

Event date： 2016.12

Language：Japanese  

Venue：九州工業大学　飯塚キャンパス   Country：Japan  

Event date： 2016.12

Language：Japanese  

Venue：九州工業大学　飯塚キャンパス   Country：Japan  

Event date： 2016.12

Language：Japanese  

Venue：九州工業大学　飯塚キャンパス   Country：Japan  

Event date： 2016.12

Language：English  

Venue：宮崎市　シーガイアコンベンションセンター   Country：Japan  

Event date： 2016.12

Language：English  

Venue：宮崎市　シーガイアコンベンションセンター   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：新科学技術推進協会（JACI）　A, B 会議室   Country：Japan  

脱細胞化組織・臓器を用いた組織構築が広く検討されている中で、我々は肝臓の構築に取り組んでいる。肝臓は酸素供給のための緻密な血管網の構築が鍵となると考え、我々は界面活性剤潅流法による微細な構造を維持したままでのラット肝臓の脱細胞化法を開発した。これを足場とした肝臓構築への取り組みについて紹介する。

Event date： 2016.11

Language：Japanese  

Venue：九州大学西新プラザ   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2016.11

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2016.10

Language：Japanese  

Venue：九州大学医学部百年講堂   Country：Japan  

Event date： 2016.9

Language：Japanese  

Venue：富山国際会議場、富山市民プラザ   Country：Japan  

Construction of organ engineered-liver which will be effective for clinical treatment

Event date： 2016.9

Language：Japanese  

Venue：九州大学   Country：Japan  

Numerical Analysis on Liver formation and Angiogenesis - Lobule and Verification -

Event date： 2016.9

Language：Japanese  

Venue：徳島大学　常三島キャンパス  理工学部・総合科学部   Country：Japan  

Event date： 2016.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2016.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2016.7

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Event date： 2016.4

Language：Japanese  

Venue：富山国際会議場、富山市民プラザ   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：大阪国際会議場   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：大阪国際会議場   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：大阪国際会議場   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：大阪国際会議場   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：関西大学　千里山キャンパス   Country：Japan  

Hybrid organoid as a functional liver tissue construct

Event date： 2016.3

Language：Japanese  

Venue：関西大学　千里山キャンパス   Country：Japan  

Study for functional evaluation of constructed liver with extracorporeal circulation

Event date： 2016.3

Language：Japanese  

Venue：佐賀大学 本庄キャンパス 理工学部大学院棟   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：福岡大学七隈キャンパス   Country：Japan  

Heparin-immobilized agarose-gelatin sponge for tissue-engineered blood vessel

Event date： 2016.3

Language：Japanese  

Venue：福岡大学七隈キャンパス   Country：Japan  

Development of sulubilized matrix derived from decellilarized porcine liver as a substratum for transplantation

Event date： 2016.3

Language：Japanese  

Venue：福岡大学七隈キャンパス   Country：Japan  

Heparin cros s linked collagen as a brain-s pecific ECM-modelized s ubstratum for neural cell culture

Event date： 2016.3

Language：Japanese  

Venue：福岡大学七隈キャンパス   Country：Japan  

Hepatocyte culture using a collagen-based material for construction recellularization liver

Event date： 2016.3

Language：Japanese  

Venue：福岡大学七隈キャンパス   Country：Japan  

Investigation of cell seeding method for transplantable recellularized liver

Event date： 2016.3

Language：Japanese  

Venue：佐賀大学 本庄キャンパス 理工学部大学院棟   Country：Japan  

Event date： 2016.3

Language：Japanese  

Venue：佐賀大学 本庄キャンパス 理工学部大学院棟   Country：Japan  

Event date： 2015.12

Language：Japanese  

Venue：宮崎大学 農学部講義棟（木花キャンパス）   Country：Japan  

Event date： 2015.12

Language：Japanese  

Venue：宮崎大学 農学部講義棟（木花キャンパス）   Country：Japan  

Event date： 2015.12

Language：Japanese  

Venue：宮崎大学 農学部講義棟（木花キャンパス）   Country：Japan  

Event date： 2015.12

Language：Japanese  

Venue：宮崎大学 農学部講義棟（木花キャンパス）   Country：Japan  

Event date： 2015.10

Language：Japanese  

Venue：城山観光ホテル   Country：Japan  

Event date： 2015.10

Language：Japanese  

Venue：城山観光ホテル   Country：Japan  

Whole organ engineering toward construction of transplantable liver

Event date： 2015.10

Language：Japanese  

Venue：九州大学伊都キャンパス椎木講堂   Country：Japan  

Event date： 2015.10

Language：Japanese  

Venue：九州大学伊都キャンパス椎木講堂   Country：Japan  

Event date： 2015.10

Language：Japanese  

Venue：九州大学伊都キャンパス椎木講堂   Country：Japan  

Event date： 2015.10

Language：Japanese  

Venue：九州大学伊都キャンパス椎木講堂   Country：Japan  

Event date： 2015.9

Language：English  

Venue：岡山国際交流センター   Country：Japan  

Event date： 2015.9

Language：English  

Venue：岡山国際交流センター   Country：Japan  

Event date： 2015.9

Language：English  

Venue：岡山国際交流センター   Country：Japan  

Event date： 2015.9

Language：English  

Venue：Boston Marriott Copley Place   Country：United States  

Event date： 2015.9

Language：English  

Venue：Boston Marriott Copley Place   Country：United States  

Event date： 2015.3

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2015.3

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2014.11

Venue：福岡   Country：Japan  

Event date： 2014.11

Venue：福岡   Country：Japan  

Event date： 2014.11

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：Daegu   Country：Korea, Republic of  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Event date： 2014.9

Venue：福岡   Country：Japan  

Language：English  

Language：Japanese  

Language：Japanese  

Language：English  

Language：English  

Language：Japanese  

Venue：鹿児島大学   Country：Japan  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Studies on decellularization and recellularization conditions of liver toward a creation clinical liver graft for transplantation

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Development of substrate having antibacterial activity for tissue-engineered artificial bile duct

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Co-culture of  hepatocyte and endothelial cell in functional gel

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Development of a novel culture method enables both in vivo-like environment and easy observation/evaluation

Language：Japanese  

Venue：北九州国際会議場   Country：Japan  

Prototype of a blood circulatable liver graft based on whole organ engineering

Language：Japanese  

Venue：九州大学西新プラザ   Country：Japan  

Language：Japanese  

Venue：福岡   Country：Japan  

Numerical Simulation of Liver Cell Proliferation with Angiogenesis　- Hepatic Lobule Formation-

Venue：熊本   Country：Japan  

Venue：熊本   Country：Japan  

Venue：熊本   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：福岡   Country：Japan  

Venue：岐阜   Country：Japan  

Venue：佐賀   Country：Japan  

Venue：佐賀   Country：Japan  

Venue：佐賀   Country：Japan  

Language：English  

Venue：熊本   Country：Japan  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

3P-246 Evaluation of a recellularized liver by applying a blood extracorporeal circulation system to hepatic failure rats

Language：English  

Language：English  

Language：Japanese  

3P-227 Whole organ engineering toward construction of transplantable liver

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

2Gp22 Basic Study for Liver Tissue Engineering by Using Decellularized Organ

Language：Japanese  

2Gp06 Development of growth factor immobilizable extracellular matrix culture substratum

Language：Japanese  

Language：Japanese  

2Jp24 Basic Study for Liver Tissue Engineering by Developing Hepatocytes-Embedded Functional Hydroeel-Filled Scaffold Culture

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：3

Type：Competition, symposium, etc. 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：2

Type：Competition, symposium, etc. 

Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

Type：Lecture

Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

Type：Lecture

Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

Type：Other