Kyushu University [Yoshihiro Izumi (Associate Professor) Medical Institute of Bioregulation, Medical Research Center for High Depth Omics]

With the recent breakthrough in metabolomics technologies, application of metabolomics has been increasing in the medical ﬁeld. Identification and semiquantitation of the compounds in the metabolome is defined as metabolic profiling, and it is applied to define metabolic changes related to genetic differences, environmental influences and disease or drug perturbations. Medical metabolomics are two major purposes for its use; the ﬁrst is to acquire knowledge on the mechanisms of drug action or the disease itself, and another is biomarker detection and disease diagnosis. Our group has developed a novel high-sensitivity and absolute quantitative metabolomics methodology based on gas chromatography, liquid chromatography, and supercritical fluid chromatography coupled to mass spectrometry. Advances in metabolic profiling offer comprehensive coverage of a metabolome as well as provide valuable insight towards understanding the diﬀerent biochemical proﬁles of a biosystem.

Research

Research Interests

Development of basic technologies for single-cell metabolomics
keyword : Single-cell metabolomics
2016.10.
Next-generation metabolomics: Technological development and medical application
keyword : Metabolomics, Metabolome analysis, Widely-targeted Quantitaive metabolomics, Non-target metabolomics
2015.04.

Academic Activities

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Reports

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Papers

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Hiroaki Takeda, Yoshihiro Izumi*, Shohei Tamura, Tomonari Koike, Yui Koike, Masashi Shiomi, Takeshi Bamba, Lipid Profiling of Serum and Lipoprotein Fractions in Response to Pitavastatin Using an Animal Model of Familial Hypercholesterolemia, Journal of Proteome Research, 10.1021/acs.jproteome.9b00602, 19, 3, 1100-1108, 2020.03, Statins are widely used for the treatment of atherosclerotic cardiovascular diseases. They inhibit cholesterol biosynthesis in the liver and cause pleiotropic effects, including anti-inflammatory and antioxidant effects. To develop novel therapeutic drugs, the effect of blood-borne lipid molecules on the pleiotropic effects of statins must be elucidated. Myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits, an animal model for hypercholesterolemia, are suitable for the determination of lipid molecules in the blood in response to statins because their lipoprotein metabolism is similar to that of humans. Herein, lipid molecules were investigated by lipidome analysis in response to pitavastatin using WHHLMI rabbits. Various lipid molecules in the blood were measured using a supercritical fluid chromatography triple quadrupole mass spectrometry. Cholesterol and cholesterol ester blood concentrations decreased by reducing the secretion of very low density lipoproteins from the liver. Independent of the inhibition effects of cholesterol biosynthesis, the concentrations of some lipids with anti-inflammation and antioxidant effects (phospholipid molecules with n-6 fatty acid side chains, lysophosphatidylcholines, phosphatidylethanolamine plasmalogens, and ceramide molecules) were significantly altered. These findings may lead to further investigation of the mechanism of statin action..

Kosuke Hata, Yoshihiro Izumi*, Takeshi Hara, Masaki Matsumoto*, Takeshi Bamba, In-Line Sample Processing System with an Immobilized Trypsin-Packed Fused-Silica Capillary Tube for the Proteomic Analysis of a Small Number of Mammalian Cells, Analytical chemistry, 10.1021/acs.analchem.9b03993, 92, 4, 2997-3005, 2020.02, Omics analysis at single-cell resolution has helped to demonstrate the shaping of cellular heterogeneity on the basis of the expression of various molecules. However, in-depth proteomic analysis of low-quantity samples has remained challenging because of difficulties associated with the measurement of large numbers of proteins by shotgun proteomics using nanoflow liquid chromatography tandem mass spectrometry (nano-LC/MS/MS). To meet such a demand, we developed a method called in-line sample preparation for efficient cellular proteomics (ISPEC) in which cells were captured, directly lysed, and digested with immobilized trypsin within fused-silica capillaries. ISPEC minimized sample loss during the sample preparation processes with a relatively small number of mammalian cells (<1000 cells) and improved the stability and efficiency of digestion by immobilized trypsin, compared to a conventional preparation method. Using our optimized ISPEC method with nano-LC/MS/MS analysis, we identified 1351, 351, and 60 proteins from 100 cells, 10 cells, and single cells, respectively. The linear response of the signal intensity of each peptide to the introduced cell number indicates the quantitative recovery of the proteome from a very small number of cells. Thus, our ISPEC strategy facilitates quantitative proteomic analysis of small cell populations..

Presentations

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Membership in Academic Society

The Mass Spectrometry Society of Japan
The Society for Biotechnology, Japan
The Japanese Biochemical Society
The Society for Chromatographic Sciences
Japan Society for Bioscience, Biotechnology, and Agrochemistry
Japan Society for SFC

Awards

Supercritical fluid chromatography/triple-quadrupole mass spectrometry-based method for highly sensitive and high-throughput analysis of multiresidue pesticides

Educational

Educational Activities

Division of Metabolomics, Graduate School of Systems Life Sciences, Kyushu University

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https://kyushu-u.pure.elsevier.com/en/persons/yoshihiro-izumi Reseacher Profiling Tool Kyushu University Pure

http://bamba-lab.com/HP: Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University .

https://kyushu-u.pure.elsevier.com/en/persons/yoshihiro-izumi
　Reseacher Profiling Tool　Kyushu University Pure

http://bamba-lab.com/
HP: Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University .