Updated on 2025/07/01

Information

 

写真a

 
HASEGAWA DAIGAKU
 
Organization
Kyushu University Hospital Endodontics Lecturer
Graduate School of Dental Science Department of Dental Science（Concurrent）
Graduate School of Dental Science （Concurrent）
School of Dentistry Department of Dentistry（Concurrent）
Title
Lecturer
Contact information
メールアドレス
Tel
0926426432
Profile
【研究活動】 ・歯根膜組織の恒常性維持におけるWnt5aの機能と役割についての研究 ・歯根膜細胞の幹細胞転換を基盤とした新規歯周組織再生法の開発研究 【臨床活動】 ・保存治療（歯内療法、修復治療） ・外科的歯内療法（歯根端切除術、意図的再植術など） ・前歯部における審美的CR修復治療 【教育活動】 ・歯学部学生（5、6年生）に対する臨床実習 ・初期研修医および後期研修医に対する卒後臨床教育 ・大学院生に対する臨床および研究指導 ・留学生に対する研究指導
External link

Degree

  • doctor of dental science

Research Interests・Research Keywords

  • Research theme： Understanding Fate Determination of Craniofacial Skeletal Stem Cells

    Keyword： Skeletal stem cells, β-catenin, GATA3

    Research period： 2020.10 - 2022.3

  • Research theme： Creation of a novel periodontal tissue regeneration method based on stem cell conversion of periodontal ligament cells.

    Keyword： periodontal stem cell, stem cell conversion, MEST

    Research period： 2018.4

  • Research theme： The role of Tenomodulin on the periodontal ligament tissue

    Keyword： Periodontal ligament, Tenomodulin

    Research period： 2016.4

  • Research theme： Development of the periodontal tissue regenerating method utilized a novel marker of periodontal ligament stem cells

    Keyword： periodontal ligament, stem cell, regeneration

    Research period： 2011.4 - 2020.9

  • Research theme： The effect of Wnt5a on human periodontal ligament cells

    Keyword： periodontal ligament, Wnt signal pathway

    Research period： 2011.4

Awards

  • 九大歯学優秀研究者賞（IF部門）

    2023.8   九州大学   Takamitsu Maruyama, Daigaku Hasegawa (Co-first author), Tomas Valenta, Jody Haigh, Maxime Bouchard, Konrad Basler, Wei Hsu. GATA3 mediates nonclassical β-catenin signaling in skeletal cell fate determination and ectopic chondrogenesis. Science Advances, Vol 8, Issue 48, eadd6172, 2022.

  • JEAワカイ賞

    2924.7   日本歯内療法学会  

    糸山知宏、虎谷渓、門脇正敬、吉田晋一郎、長谷川大学、杉井英樹、兼子大志、前田英史

  • 優秀ポスター賞

    2016.10   日本歯科保存学会  

  • JEA会長賞

    2016.7   日本歯内療法学会  

  • 優秀ポスター賞

    2015.10   日本歯科保存学会  

  • 優秀ポスター賞

    2014.10   日本歯科保存学会  

  • 大学院中間発表賞

    2014.2   九州大学歯学部  

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Papers

  • GATA3 mediates nonclassical β-catenin signaling in skeletal cell fate determination and ectopic chondrogenesis Reviewed International journal

    Maruyama T (Co-First), Hasegawa D (Co-First), Valenta T, Haigh J, Bouchard M, Basler K, Hsu W

    Science Advances   2022.12

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues Reviewed International journal

    Kaneko H, Hasegawa D, Itoyama T, Yoshida S, Tomokiyo A, Hamano S, Sugii H, Maeda H

    Archives of oral biology   2022.2

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers Reviewed International journal

    Tomokiyo A, Hasegawa D, Ono T, Nagano R, Ipposhi K, Yamashita K, Alhasan MA, Maeda H

    Odontology   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  • MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells Reviewed International journal

    Daigaku Hasegawa, Kana Hasegawa, Hiroshi Kaneko, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Tamotsu Kiyoshima, Hidefumi Maeda

    Stem Cells International   2020.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1155/2020/9672673

  • R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway Reviewed International journal

    Mai Arima, Daigaku Hasegawa, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Hidefumi Maeda

    Journal of Periodontal Research   2019.4

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling Invited Reviewed International journal

    Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda

    Journal of Cellular Physiology   2017.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through TGFβ1-mediated Upregulation of Periostin Expression

    Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Satoshi Monnouchi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine

    Journal of Cellular Physiology   2015.2

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    Language：English  

    DOI： 10.1002/jcp.24950

  • Establishment of Periodontal Ligament Stem Cell-like Cells Derived from Feeder-Free Cultured Induced Pluripotent Stem Cells Reviewed

    Yamashita, D; Hamano, S; Hasegawa, D; Sugii, H; Itoyama, T; Ikeya, M; Maeda, H

    STEM CELLS AND DEVELOPMENT   2024.12

  • Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone Reviewed

    Albougha, MS; Sugii, H; Adachi, O; Mardini, B; Soeno, S; Hamano, S; Hasegawa, D; Yoshida, S; Itoyama, T; Obata, J; Maeda, H

    BIOMOLECULES   2024.11

  • 4-META/MMA-TBB resin containing nano hydroxyapatite induces the healing of periodontal tissue repair in perforations at the pulp chamber floor

    Sugii, H; Yoshida, S; Albougha, MS; Hamano, S; Hasegawa, D; Itoyama, T; Obata, J; Kaneko, H; Minowa, F; Tomokiyo, A; Maeda, H

    CELL BIOCHEMISTRY AND FUNCTION   42 ( 4 )   e4058   2024.6   （ ISSN:0263-6484 eISSN:1099-0844 ）

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    Language：English   Publisher：Cell Biochemistry and Function  

    We aimed to evaluate the materials based on 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane (Super-bond [SB]) and nano hydroxyapatite (naHAp) for the repair of perforation at pulp chamber floor (PPF) in vitro and in vivo models. SB and naHAp were mixed in the mass ratio of 10% or 30% to produce naHAp/SB. Human periodontal ligament stem cells (HPDLSCs) were cultured on resin discs of SB or naHAp/SB to analyze the effects of naHAp/SB on cell adhesion, proliferation, and cementoblastic differentiation. A rat PPF model was treated with SB or naHAp/SB to examine the effects of naHAp/SB on the healing of defected cementum and periodontal ligament (PDL) at the site of PPF. HPDLSCs were spindle-shaped and adhered to all resin discs. Changing the resin from SB to naHAp/SB did not significantly alter cell proliferation. Both 10% and 30% naHAp/SB were more effective than SB in promoting cementoblastic differentiation of HPDLSCs. In the rat PPF model, 30% naHAp/SB was more effective than SB in promoting the formation Sharpey's fiber-like structures with expression of the PDL-related marker and cementum-like structures with expression of cementum-related markers. In conclusion, 30% naHAp/SB can be the new restorative material for PPF because it exhibited the abilities of adhering to dentin and healing of defected periodontal tissue.

    DOI： 10.1002/cbf.4058

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  • Effect of Fibrillin-2 on Differentiation into Periodontal Ligament Stem Cell-Like Cells Derived from Human-Induced Pluripotent Stem Cells

    Hamano, S; Yamashita, D; Hasegawa, D; Sugii, H; Itoyama, T; Maeda, H

    STEM CELLS AND DEVELOPMENT   33 ( 9-10 )   228 - 238   2024.5   （ ISSN:1547-3287 eISSN:1557-8534 ）

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    Language：English   Publisher：Stem Cells and Development  

    Periodontal tissue regeneration is important for preserving teeth. Periodontal ligament stem cells (PDLSCs) are useful in periodontal tissue regeneration; however, tooth extraction is required to obtain these cells. Therefore, we focused on induced pluripotent stem (iPS) cells and established a method to obtain PDLSC-like cells from iPS cells. Specifically, we first differentiated iPS cells into neural crest-like cells (iNCs). Next, we obtained PDLSC-like cells (iPDLSCs) by culturing iNCs on extracellular matrix (ECM) derived from human primary periodontal ligament cells (HPDLCs). This differentiation method suggested that ECM derived from HPDLCs is important for iPDLSC differentiation. Thus, we aimed to identify the PDLSC-inducing factor present in HPDLC-derived ECM in this study. We first performed comprehensive analyses of HPDLC genes and identified fibrillin-2 (FBN2), an ECM-related factor. Furthermore, to clarify the effect of FBN2 on iPDLSC differentiation, we cultured iNCs using ECM derived from HPDLCs with FBN2 knocked down. As a result, expression of PDL-related markers was reduced in iNCs cultured on ECM derived from HPDLCs transfected with FBN2 siRNA (iNC-siFBN2) compared with iPDLSCs. Furthermore, the expression of CD105 (a mesenchymal stem cell marker), proliferation ability, and multipotency of iNC-siFBN2 were lower compared with iPDLSCs. Next, we cultured iNCs on FBN2 recombinant protein; however, expression of PDL-related markers did not increase compared with iPDLSC. The present results suggest the critical involvement of FBN2 in inducing iPDLSCs from iNCs when in fact it does not promote iPDLSC differantiation. Therefore, we need to elucidate the entire HPDLC-ECMs, responsible for iPDLSCs induction.

    DOI： 10.1089/scd.2024.0013

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  • Effect of fibrillin-2 on differentiation into periodontal ligament stem cell-like cells derived from human-induced pluripotent stem cells Reviewed International journal

    Hamano S, Yamashita D, Hasegawa D, Sugii H, Itoyama T, Maeda H

    Stem Cells Dev   2024.3

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  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

    Fujino, S; Hamano, S; Tomokiyo, A; Sugiura, R; Yamashita, D; Hasegawa, D; Sugii, H; Fujii, S; Itoyama, T; Miyaji, H; Maeda, H

    SCIENTIFIC REPORTS   13 ( 1 )   5668   2023.4   （ ISSN:2045-2322 ）

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    Language：English   Publisher：Scientific Reports  

    Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

    DOI： 10.1038/s41598-023-32126-1

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  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells Reviewed International journal

    Fujino S, Hamano S, Tomokiyo A, Sugiura R, Yamashita D, Hasegawa D, Sugii H, Fujii S, Itoyama T, Miyaji H, Maeda H

    Sci Rep   2023.4

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  • Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Reviewed International journal

    Naati Fakatava, Hiromi Mitarai, Asuka Yuda, Akira Haraguchi, Hiroko Wada, Daigaku Hasegawa, Hidefumi Maeda, Naohisa Wada

    J Dent Sci   2023.4

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  • Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway

    Fakatava, N; Mitarai, H; Yuda, A; Haraguchi, A; Wada, H; Hasegawa, D; Maeda, H; Wada, N

    JOURNAL OF DENTAL SCIENCES   18 ( 2 )   567 - 576   2023.4   （ ISSN:1991-7902 eISSN:2213-8862 ）

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    Language：English   Publisher：Journal of Dental Sciences  

    Background/purpose: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells. Materials and methods: RNA expression was determined using semi-quantitative reverse transcription–polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells. Results: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2–23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2–23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1–stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3. Conclusion: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies.

    DOI： 10.1016/j.jds.2022.08.030

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  • Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells

    Adachi, O; Sugii, H; Itoyama, T; Fujino, S; Kaneko, H; Tomokiyo, A; Hamano, S; Hasegawa, D; Obata, J; Yoshida, S; Kadowaki, M; Sugiura, R; Albougha, MS; Maeda, H

    MOLECULES   27 ( 23 )   2022.12   （ eISSN:1420-3049 ）

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    Language：English   Publisher：Molecules  

    The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats by immunohistochemistry and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs) and preosteoblasts by Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, and western blotting. The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery compared with the nonwounded PDL tissue, whereas its expression was not changed in the osteoblastic layer around the wounded alveolar bone. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs, but it did not affect the osteoblastic differentiation of preosteoblasts. ERK1/2 phosphorylation was upregulated during the DCN-induced osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation through ERK1/2 signaling in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.

    DOI： 10.3390/molecules27238224

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  • Current Application of iPS Cells in the Dental Tissue Regeneration

    Hamano, S; Sugiura, R; Yamashita, D; Tomokiyo, A; Hasegawa, D; Maeda, H

    BIOMEDICINES   10 ( 12 )   2022.12   （ ISSN:2227-9059 eISSN:2227-9059 ）

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  • Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells Reviewed International journal

    Adachi O, Sugii H, Itoyama T, Fujino S, Kaneko H, Tomokiyo A, Hamano S, Hasegawa D, Obata J, Yoshida S, Kadowaki M, Sugiura R, Albougha MS, Maeda H

    Molecules   2022.11

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  • Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation Reviewed International journal

    Kadowaki M, Yoshida S, Itoyama T, Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Kaneko H, Sugiura R, Maeda H

    Life (Basel)   2022.11

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  • Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation

    Kadowaki, M; Yoshida, S; Itoyama, T; Tomokiyo, A; Hamano, S; Hasegawa, D; Sugii, H; Kaneko, H; Sugiura, R; Maeda, H

    LIFE-BASEL   12 ( 11 )   2022.11   （ ISSN:2075-1729 eISSN:2075-1729 ）

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    In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.

    DOI： 10.3390/life12111812

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  • PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix

    Sugiura, R; Hamano, S; Tomokiyo, A; Hasegawa, D; Yoshida, S; Sugii, H; Fujino, S; Adachi, O; Kadowaki, M; Yamashita, D; Maeda, H

    BIOMEDICINES   10 ( 10 )   2022.10   （ ISSN:2227-9059 eISSN:2227-9059 ）

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    Language：English   Publisher：Biomedicines  

    Periodontal ligament stem cells (PDLSCs) play central roles in periodontal ligament (PDL) tissue homeostasis, repair, and regeneration. Previously, we established a protocol to differentiate human-induced pluripotent stem cell-derived neural crest-like cells (iNCs) into PDLSC-like cells (iPDLSCs) using human PDL cell-derived extracellular matrix (ECM). However, it remained unclear what factors principally regulate the differentiation of iNCs into iPDLSCs. In this study, we aimed to identify the transcription factor regulating production of human PDL cell-derived ECM, which is responsible for the generation of iPDLSCs. We cultured iNCs on ECMs of two human PDL cell lines (HPDLC-3S and HPDLC-3U) and of human dermal fibroblasts (HDF). iNCs cultured on HPDLC-3U demonstrated higher iPDLSC-associated gene expression and mesenchymal differentiation capacity than cells cultured on HDF or HPDLC-3S. The transcription factor PAX9 was highly expressed in HPDLC-3U compared with HDF and HPDLC-3S. iNCs cultured on siPAX9-transfected HPDLC-3U displayed downregulation of iPDLSC-associated marker expression and adipocytic differentiation capacity relative to controls. Our findings suggest that PAX9 is one of the transcription factors regulating ECM production in human PDL cells, which is responsible for the differentiation of iNCs into iPDLSCs.

    DOI： 10.3390/biomedicines10102366

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  • PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix Reviewed International journal

    Sugiura R, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Fujino S, Adachi O, Kadowaki M, Yamashita D, Maeda H

    Biomedicines   2022.9

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  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues

    Kaneko, H; Hasegawa, D; Itoyama, T; Yoshida, S; Tomokiyo, A; Hamano, S; Sugii, H; Maeda, H

    ARCHIVES OF ORAL BIOLOGY   134   105323   2022.2   （ ISSN:0003-9969 eISSN:1879-1506 ）

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    Language：English   Publisher：Archives of Oral Biology  

    Objectives: Few clinical treatments to regenerate periodontal tissue lost due to severe endodontic and periodontal disease have yet been developed. Therefore, the development of new treatment methods for the regeneration of periodontal tissue is expected. The purpose of this study was to investigate the effects of a c-Jun N-terminal kinase (JNK) inhibitor, SP600125, on the osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro, and the function of SP600125 on the regeneration of alveolar bone in vivo. Design: Alizarin red S staining, quantitative RT-PCR, and western blotting analysis was performed to determine whether SP600125 affects osteoblastic differentiation of human PDLSCs (HPDLSCs) and bone-related intracellular signaling. The effect of SP600125 on the regeneration of alveolar bone was assessed by using a rat periodontal defect model. The healing of periodontal defects was evaluated using micro-CT scans and histological analysis. Results: SP600125 promoted the osteoblastic differentiation such as Alizarin red S-positive mineralized nodule formation and the expression of osteoblast-related genes in HPDLSCs under osteogenic conditions. In addition, this inhibitor upregulated the BMP2 expression and the phosphorylation of Smad1/5/8 in HPDLSCs under the same conditions. The inhibition of Smad1/5/8 signaling by LDN193189 suppressed the SP600125-induced osteoblastic differentiation of HPDLSCs. Furthermore, the application of SP600125 promoted the regeneration of not only alveolar bone but also PDL tissue in periodontal defects. Conclusion: This study suggested that inhibition of JNK signaling promotes the osteoblastic differentiation of HPDLSCs through BMP2-Smad1/5/8 signaling, leading to the regeneration of periodontal tissues such as alveolar bone and PDL tissue.

    DOI： 10.1016/j.archoralbio.2021.105323

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  • Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers

    Tomokiyo, A; Hasegawa, D; Ono, T; Nagano, R; Ipposhi, K; Yamashita, K; Alhasan, MA; Maeda, H

    ODONTOLOGY   110 ( 1 )   127 - 137   2022.1   （ ISSN:1618-1247 eISSN:1618-1255 ）

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    Language：English   Publisher：Odontology  

    The aim of this study was to characterize a clonal human periodontal ligament (PDL) stem cell line (line 2–23 cells) cultured with root canal sealers based on methacrylate resin (SuperBond sealer; SB), bioactive glass (Nishika Canal Sealer BG; BG), or silicon (GuttaFlow 2; GF). The sealers were set in rubber molds to form sealer discs. Line 2–23 cells were cultured with or without the discs for 3 days. The cell viability was evaluated by direct cell counting and MTT assay. Inflammation-, PDL-, collagen-, and cell cycle-related gene expression was investigated by real-time RT-PCR. Collagen production was analyzed by Picro Sirius Red staining. Calcium ion concentration in the culture was measured by a QuantiChrom calcium assay kit. Line 2–23 cells survived when cultured with GF discs, but decreased cell viability was observed with SB and BG discs. The expression of inflammation-related genes was higher in cells cultured with SB discs, and expression of PDL-related genes was lower in cells exposed to SB and BG discs. These discs also down-regulated collagen production in line 2–23 cells. BG discs increased calcium ion concentration in the culture medium. Cells exposed to GF discs exhibited the same inflammation-, PDL-, collagen-, and cell cycle-related gene expression and collagen production as untreated cells. These results suggested that the characteristics of line 2–23 cells cultured with GF discs was highly resemble to untreated cells throughout the 3 days of the culture model.

    DOI： 10.1007/s10266-021-00648-7

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  • Activin A Promotes Osteoblastic Differentiation of Human Preosteoblasts through the ALK1-Smad1/5/9 Pathway Reviewed International journal

    Sugii H, Albougha MS, Adachi O, Tomita H, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Itoyama T, Maeda H

    Int J Mol Sci.   2021.12

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  • Development of a novel direct pulp-capping material using 4-META/MA-TBB resin with nanohydroxyapatite Reviewed International journal

    Yoshida S, Sugii H, Itoyama T, Kadowaki M, Hasegawa D, Tomokiyo A, Hamano S, Ipposhi K, Yamahita K, Maeda H

    Mater Sci Eng C Mater Biol Appl.   2021.9

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  • Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells Reviewed International journal

    Ipposhi K, Tomokiyo A, Ono T, Yamashita K, Anas MA, Hasegawa D, Hamano S, Yoshida S, Sugii H, Itoyama T, Ogawa M, Maeda H

    Cells   2021.9

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  • Possible function of GDNF and Schwann cells in wound healing of periodontal tissue Reviewed International journal

    Itoyama T, Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Ono T, Fujino S, Maeda H

    J Periodont Res.   2020.12

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  • Functions of beta2-adrenergic receptor in human periodontal ligament cells Reviewed International journal

    Hamano S, Tomokiyo A, Hasegawa D, Yuda A, Sugii H, Yoshida S, Mitarai H, Wada N, Maeda H

    J Cell Biochem.   2020.3

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 未重合レジンセメントと未重合フロアブルレジンの結合力 Reviewed

    杉井英樹、吉田晋一郎、友清淳、濱野さゆり、長谷川大学、前田英史

    日歯保存誌   2020.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Expression and function of dopamine in odontoblasts Invited Reviewed International journal

    Fujino S, Hamano S, Tomokiyo A, Itoyama T, Hasegawa D, Sugii H, Yoshida S, Washio A, Nozu A, Ono T, Wada N, Kitamura C, Maeda H

    J Cell Physiol.   2019.10

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  • 骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する Reviewed

    吉田晋一郎、和田尚久、長谷川大学、御手洗裕美、有馬麻衣、友清淳、濱野さゆり、杉井英樹、前田英史

    日本歯科保存学会誌   2018.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Senescence and odontoblastic differentiation of dental pulp cells Reviewed

    Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hiromi Mitarai, Shuntaro Taniguchi, Naohisa Wada, Hidefumi Maeda

    Journal of cellular physiology   234 ( 1 )   849 - 859   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor-α (TNF-α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF-α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence-related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblast-inductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF-α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF-α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF-α-treated sHDPCs, whereas restored the reduction in TNF-α-treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF-α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF-α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF-α.

    DOI： 10.1002/jcp.26905

  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells Invited Reviewed International journal

    Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H

    Stem cells and development   2017.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • 根管治療ならびに修復処置関連溶液によって生じるWhite Mineral Trioxide Aggregateの色調変化に関する比較分析 Invited Reviewed International journal

    友清 淳, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 御手洗 裕美, 有馬 麻衣, 野津 葵, 和田尚久, 前田 英史

    日本歯科保存学会誌   60 ( 4 )   200 - 210   2017.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells Invited Reviewed International journal

    Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda

    Bone   2017.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells Invited Reviewed International journal

    Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda

    Journal of Periodontal research   2017.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells Invited Reviewed International journal

    Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Hiroyuki Mizumachi, Hiromi Mitarai, Satoshi Monnouchi, Naohisa Wada, Hidefumi Maeda

    Archives of oral biology   2017.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • GDNF from Human Periodontal Ligament Cells Treated with Proinflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells Reviewed International journal

    Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Satoshi Monnouchi, Asuka Yuda, Hidefumi Maeda

    Journal of Cellular Biochemistry   2016.7

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  • Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells Reviewed International journal

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Hirofumi Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

    Journal of Dental Research   2016.6

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  • Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells Reviewed International journal

    Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P. Mark Bartold, Danijela Menicanin, Fujii Shinsuke, Shinichiro Yoshida, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine

    Stem Cells and Development   2014.9

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  • The role of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells Invited Reviewed International journal

    Koori K, Maeda H, Fujii S, Tomokiyo A, Kawachi G, Hasegawa D, Hamano S, Sugii H, Wada N, Akamine A

    Cell and Tissue Research   2014.8

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  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells Reviewed International journal

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohide Yamamoto, Naohisa Wada, Katsuaki Koori, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Satoshi Monnouchi, Akifumi Akamine

    Bone   2014.6

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  • Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of a human periodontal ligament stem/progenitor cell line Reviewed International journal

    Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Satoshi Monnouchi, Naohide Yamamoto, Naohisa Wada, Katsuaki Koori, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Akifumi Akamine

    Journal of Cellular Physiology   2014.6

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Presentations

  • BMP4によるヒト歯根膜細胞の幹細胞転換の可能性, 第23回日本再生医療学会総会

    長谷川大学, 田下滉大, Yuxin Huang, 兼子大志, 前田英史

    第23回日本再生医療学会総会  2024.3 

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    Event date： 2024.3

    Language：Japanese  

    Country：Japan  

  • c-Jun N-terminal kinase阻害剤を用いた歯周組織再生過程における抗炎症作用の検討

    兼子大志、長谷川大学、糸山知宏、門脇正敬、前田英史

    第23回日本再生医療学会総会  2024.3 

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    Event date： 2024.3

    Language：Japanese  

    Country：Japan  

  • Cadherin-2はヒト歯根膜細胞の幹細胞転換に関与する

    長谷川大学、田下滉大、Yuxin Huang、兼子大志、前田英史

    第159回日本歯科保存学会秋季学術大会  2023.11 

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    Event date： 2023.11

    Language：Japanese  

    Country：Japan  

  • Tenomodulinはヒト歯根膜細胞のコラーゲン産生に関与する

    長谷川大学、田下晃大、兼子大志、前田英史

    第157回日本歯科保存学会秋季学術大会  2022.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • レジン系、バイオガラス系およびシリコン系根管充填用シーラーのヒト歯根膜幹細胞株に対する細胞親和性比較

    友清淳、長谷川大学、小野太雅、一法師啓太、山下梢、M. Anas Alhasan、濱野さゆり、杉井英樹、吉田晋一郎、前田英史

    第152回日本歯科保存学会春季学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • JNK阻害による歯根膜幹細胞の骨芽細胞分化促進のメカニズム解析および歯周組織再生への影響

    兼子大志、長谷川大学、糸山知宏、吉田晋一郎、友清淳、濱野さゆり、杉井英樹、前田英史

    第155回日本歯科保存学会秋季学術大会  2021.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Inhibition of c-Jun N-terminal kinase affects regeneration of periodontal tissues International conference

    Kaneko H, Hasegawa D, Itoyama T, Yoshida S, Tomokiyo A, Hamano S, Sugii H, Maeda H

    KOB & OBT 5th Joint International Symposium  2021.11 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • Non-canonical Wntシグナル経路の阻害が未分化なヒト歯根膜細胞株の骨芽細胞様分化に及ぼす影響

    兼子大志、長谷川大学、糸山知宏、友清淳、濱野さゆり、吉田晋一郎、杉井英樹、前田英史

    日本歯科保存学会2019年度秋季学術大会  2019.11 

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    Event date： 2019.12

    Language：Japanese  

    Venue：福岡国際会議場（福岡市）   Country：Japan  

  • LGR4が未分化なヒト歯根膜細胞の増殖能、走化性および骨芽細胞様分化に及ぼす影響

    有馬麻衣、長谷川大学、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、杉井英樹、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会  2018.11 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都市勧業館みやこめっせ   Country：Japan  

  • 新規幹細胞関連因子MESTがヒト歯根膜細胞の幹細胞転換に及ぼす影響

    長谷川大学、長谷川佳那、御手洗裕美、有馬麻衣、濱野さゆり、吉田晋一郎、友清淳、杉井英樹、和田尚久、清島保、前田英史

    日本歯科保存学会2018年度春季学術大会  2018.6 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜みなとみらいホール   Country：Japan  

  • ヒト歯根膜幹細胞における新規幹細胞特性制御因子としてのMESTの可能性

    長谷川大学、御手洗裕美、長谷川佳那、有馬麻衣、濱野さゆり、吉田晋一郎、友清淳、杉井英樹、和田尚久、清島保、前田英史

    第17回日本再生医療学会総会  2018.3 

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    Event date： 2018.3

    Language：Japanese  

    Venue：パシフィコ横浜（横浜市）   Country：Japan  

  • R-spondin2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the canonical Wnt signaling pathway International conference

    Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H

    Kyudai Oral Bioscience 2018 -12nd International Symposium-  2018.2 

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    Event date： 2018.2

    Language：English  

    Venue：Fukuoka Recent Hotel, Fukuoka   Country：Japan  

  • R-spondin2はカノニカルWntシグナルを介して未分化なヒト歯根膜細胞の骨芽細胞様分化を促進する

    有馬麻衣、長谷川大学、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、杉井英樹、和田尚久、前田英史

    第147回日本歯科保存学会秋季学術大会  2017.10 

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    Event date： 2017.10 - 2018.10

    Language：Japanese  

    Venue：マリオス（盛岡市）   Country：Japan  

  • Tenomodulinがヒト歯根膜細胞の機能維持に及ぼす影響について

    長谷川大学、和田尚久、有馬麻衣、吉田晋一郎、友清淳、濱野さゆり、御手洗裕美、前田英史

    第146回日本歯科保存学会春季学術大会  2017.6 

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    Event date： 2017.6

    Language：Japanese  

    Venue：リンクステーションホール青森（青森市）   Country：Japan  

  • R-spondin2が未分化なヒト歯根膜細胞の線維芽細胞様分化に及ぼす影響

    園田麻衣、長谷川大学、和田尚久、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、前田英史

    第145回日本歯科保存学会秋季学術大会  2016.10 

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    Event date： 2016.10 - 2018.10

    Language：Japanese  

    Venue：キッセイ文化ホール（松本市）   Country：Japan  

  • Identification of a novel periodontal ligament stem cell marker International conference

    Hasegawa D, Wada N, Hamano S, Tomokiyo A, Yoshida S, Mitarai H, Sonoda M, Sugii H, Maeda H

    94th General Session & Exhibition of the IADR  2016.6 

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    Event date： 2016.6 - 2018.6

    Language：English  

    Venue：COEX Convention & Exhibition Center (Seoul)   Country：Japan  

  • MESTはヒト歯根膜幹細胞における幹細胞特性の維持に関与する

    長谷川大学、和田尚久、濱野さゆり、友清淳、吉田晋一郎、御手洗裕美、園田麻衣、杉井英樹、前田英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date： 2016.6

    Language：Japanese  

    Venue：栃木県総合文化センター（宇都宮市）   Country：Japan  

  • JNK阻害剤はヒト歯髄幹細胞の象牙芽細胞様分化を促進する

    黄玉鑫、長谷川大学、田下滉大、兼子大志、前田英史

    第161回日本歯科保存学会秋季学術大会  2024.11 

  • 細胞接着分子ICAM2が歯髄細胞に及ぼす影響について

    田下滉大、長谷川大学、兼子大志、黄玉鑫、前田英史

    第45回日本歯内療法学会学術大会  2024.7 

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    Presentation type：Poster presentation  

  • 下顎管に交通した根尖病変を有する下顎第一大臼歯に対してElectro-Magnetic Apical Treatment（EMAT）を併用して治癒を図った一症例

    木下康平、長谷川大学、兼子大志、鄭霞暎、前田英史

    第161回日本歯科保存学会秋季学術大会  2024.11 

  • The effects of Wnt5a on Human Periodontal Ligament Cells

    Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Satoshi　Monnouchi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine

    93rd General Session & Exhibition of the IADR  2015.3 

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    Language：English  

    Country：United States  

  • 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション

    長谷川 大学, 和田 尚久, 前田 英史, 藤井 慎介, 郡 勝明, 友清 淳, 門野内 聡, 河野 清美, 山本 直秀, 寺松 陽子, 濱野 さゆり, 祐田 明香, 赤峰 昭文

    第137回 日本歯科保存学会秋季学術大会  2012.11 

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    Language：Japanese  

    Country：Japan  

  • Wnt5aがヒト歯根膜細胞に及ぼす影響について

    長谷川 大学, 和田 尚久, 前田 英史, 友清 淳, 門野内 聡, 郡 勝明, 吉田 晋一郎, 寺松 陽子, 濱野 さゆり, 祐田 明香, 杉井 英樹, 赤峰 昭文

    第139回 日本歯科保存学会秋季学術大会  2013.10 

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    Language：Japanese  

    Country：Japan  

  • The Efects of Wnt5a on Human Periodontal Ligament Cells

    Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Satoshi　Monnouchi, Katsuaki Koori, Sayuri Hamano, Hiromi Mitarai, Akifumi Akamine

    Kyudai Oral Bioscience 2014 -8th International Symposium  2014.2 

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    Language：English  

    Country：Japan  

  • Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する

    長谷川 大学, 和田 尚久, 前田 英史, 吉田 晋一郎, 門野内 聡, 御手洗 裕美, 濱野 さゆり, 祐田 明香, 赤峰 昭文

    第140回 日本歯科保存学会春季学術大会  2014.6 

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    Language：Japanese  

    Country：Japan  

  • Wnt5aはRor2-JNKシグナルを介してヒト歯根膜幹細胞の骨芽細胞様分化を抑制する

    長谷川 大学, 和田 尚久, 前田 英史, 吉田 晋一郎, 御手洗 裕美, 門野内 聡, 濱野 さゆり, 祐田 明香, 赤峰 昭文

    第141回 日本歯科保存学会秋季学術大会  2014.10 

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    Language：Japanese  

    Country：Japan  

  • Exosomes from human periodontal ligament stem cells promote bone healing International conference

    Albougha MS, Sugii H, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Obata J, Itoyama T, Kaneko H, Kadowaki M, Kinoshita K, Maeda H

    The 71st Annual Meeting of Japanese Association for Dental Research  2023.11 

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    Event date： 2023.11

    Language：English  

    Country：Japan  

  • The 4-META/MMA-TBB Resin Containing Nano Hydroxyapatite Regenerates Wounded Periodontal Tissue At The Perforation Site International conference

    Sugii S, Yoshida S, Hamano S, Hasegawa D, Obata J, Itoyama T, Albougha MS, Minowa F, Tomokiyo A, Maeda H

    The 22nd Scientific Congress of Asian Pacific Endodontic Confederation  2023.8 

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    Event date： 2023.8

    Language：English  

    Country：Taiwan, Province of China  

  • NaClOに浸漬したMineral Trioxide Aggregateはヒト歯根膜幹細胞株の骨芽細胞分化能を低下させる

    山下梢、友清淳、濱野さゆり、 長谷川大学、杉井英樹、吉田晋一郎、小野太雅、一法師啓太、M.Anas Alhasan、前田英史

    第152回日本歯科保存学会春季学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Transgelinは、Integrinを介した細胞外基質への接着に関与する

    御手洗裕美、祐田明香、Naati Fakatava、長谷川大学、前田英史、和田尚久

    第152回日本歯科保存学会春季学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • The Role of ACTA2 in Periodontal Ligament Cell Stimulated with TGF-beta1

    Naati Fakatava、御手洗裕美、祐田明香、長谷川大学、前田英史、和田尚久

    第152回日本歯科保存学会春季学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • フェノール系貼薬剤は硬化後のWhite Mineral Trioxide Aggregateの崩壊および色調変化を誘導する

    友清淳、山下梢、小野太雅、一法師啓太、濱野さゆり、長谷川大学、杉井英樹、吉田晋一郎、前田英史

    第41回日本歯内療法学会学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • sFRP1は歯髄細胞の象牙芽細胞様分化を誘導し、デンティンブリッジ形成を促進する

    一法師啓太、友清淳、小野太雅、山下梢、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、小川真里奈、前田英史

    第41回日本歯内療法学会学術大会  2020.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Decorinが未分化なヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響

    足立織利恵、杉井英樹、糸山知宏、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、Mhd Safwan Albougha、前田英史

    第153回日本歯科保存学会秋季学術大会  2020.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • 直接覆髄後の歯髄組織におけるM2マクロファージの集簇, , 2020.11.

    門脇正敬、吉田晋一郎、糸山知宏、友清淳、濱野さゆり、長谷川大学、杉井英樹、前田英史

    第153回日本歯科保存学会秋季学術大会  2020.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • iPS細胞から歯根膜幹細胞様細胞への分化誘導能を有する転写因子の同定

    杉浦梨紗、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、前田英史

    第153回日本歯科保存学会秋季学術大会  2020.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation by regulating Notch signaling in dental pulp cells International conference

    Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, M, Anas Alhasan, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Hidefumi Maeda

    Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2021  2021.2 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • Nano hydroxyapatite含有4-META/MMA-TBBレジンは、Calcium-sensing receptor-MEK/ERK経路を介してヒト歯髄幹細胞の象牙芽細胞様分化を促進する

    吉田晋一郎、杉井英樹、糸山知宏、門脇正敬、一法師啓太、山下梢、友清淳、濱野さゆり、長谷川大学、前田英史

    第154回日本歯科保存学会春季学術大会  2021.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Hyaluronic Acid Could Enhance the Differentiation of Neural Crest like Cells to Periodontal Ligament Stem Cells

    M Anas Alhasan, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Taiga Ono, Keita Ipposhi, Kozue Yamashita, Hidefumi Maeda

    第154回日本歯科保存学会春季学術大会  2021.6 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • 4-META/MMA-TBB resin including nano-hydroxyapatite as a direct pulp capping material International conference

    Shinichiro Yoshida, Hideki Sugii, Masataka Kadowaki, Tomohiro Itoyama, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Keita Ipposhi, Kozue Yamashita, Hidefumi Maeda

    99th General Session & Exhibition of the IADR  2021.7 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • Activin A mediates the ALK1-Smad1/5/9 pathway during osteoblastic differentiation of human pre-osteoblasts International conference

    Hideki Sugii, Mhd Safwan Albougha, Orie Adachi, Hiroka Tomita, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Junko Obata, Tomohiro Itoyama, Taiga Ono, Keita Ippoushi and Hidefumi Maeda

    99th General Session & Exhibition of the IADR  2021.7 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • WMTA の組成成分に及ぼすフェノール系貼薬剤の影響について

    友清淳、山下梢、小野太雅、一法師啓太、濱野さゆり、長谷川大学、杉井英樹、吉田晋一郎、前田英史

    第42回日本歯内療法学会学術大会・第24回日本歯科医学会学術大会併催  2021.9 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Activin Aによるヒト前骨芽細胞の分化誘導に関わるシグナル伝達の解析

    杉井英樹、Mhd Safwan Albougha、足立織利恵、富田洋嘉、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、糸山知宏、小幡純子、前田英史

    第155回日本歯科保存学会秋季学術大会  2021.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Activin Aがヒト歯髄細胞の象牙芽細胞様分化に及ぼす影響について

    富田洋嘉、杉井英樹、門脇正敬、Mhd Safwan Albougha、足立織利恵、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、糸山知宏、小幡純子、前田英史

    第155回日本歯科保存学会秋季学術大会  2021.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation in dental pulp cells International conference

    Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, M. Anas Alhasan, Hirofumi Miyaji, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Tsutomu Sugaya, Hidefumi Maeda

    The International Scientific Meeting of 30th Anniversary of Korean Academy of Endodontics & the 19th JEA-KAE Joint Scientific Meeting  2021.10 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • iPS細胞から歯根膜幹細胞様細胞への分化誘導能を有する候補因子の同定

    杉浦梨紗、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、山下大輝、前田英史

    第21回日本再生医療学会総会  2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts

    Mhd Safwan Albougha, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Taiga Ono, Orie Adachi, Hidefumi Maeda

    第156回日本歯科保存学会春季学術大会  2022.6 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • Decorinによるヒト歯根膜幹細胞の骨芽細胞様分化誘導に関する分子機構の解析

    足立織利恵、杉井英樹、糸山知宏、兼子大志、友清淳、濱野さゆり、長谷川大学、小幡純子、吉田晋一郎、藤野翔香、Mhd Safwan Albougha、前田英史

    第156回日本歯科保存学会春季学術大会  2022.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • ACTA2 regulates human PDL function via interaction with or without TGF-β1

    Naati Fakatava、御手洗裕美、祐田明香、原口晃、長谷川大学、前田英史、和田尚久

    第156回日本歯科保存学会春季学術大会  2022.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • ドーパミンは修復象牙質形成能を有する

    藤野翔香、杉浦梨沙、濱野さゆり、友清淳、山下大樹、長谷川大学、杉井英樹、糸山知宏、前田英史

    第３回象牙質歯髄治療学会学術大会  2022.9 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • フェノール系貼薬剤はケイ酸カルシウムの体積増加を介して硬化後のWhite Mineral Trioxide Aggregateの崩壊を誘導し封鎖性を低下させる

    友清淳、山下梢、小野太雅、一法師啓太、M.Anas Alhasan、濱野さゆり、長谷川大学、杉井英樹、前田英史

    第157回日本歯科保存学会秋季学術大会  2022.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Hyaluronic Acid Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells

    M Anas Alhasan, Atushi Tomokiyo, Taiga Ono, Keita Ippoushi, Kozue Yamashita, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Hidefumi Maeda

    第157回日本歯科保存学会秋季学術大会  2022.11 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • セメント質誘導型ナノハイドロキシアパタイト含有レジンの新規開発

    杉井英樹、吉田晋一郎、Mhd Safwan Albougha、足立織利恵、友清淳、濱野さゆり、長谷川大学、前田英史

    第22回日本再生医療学会総会  2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • 継代培養したiPS細胞由来神経堤細胞様細胞の歯根膜幹細胞分化能について

    杉浦梨沙、濱野さゆり、山下大輝、友清淳、長谷川大学、吉田晋一郎、杉井英樹、藤野翔香、前田英史

    第22回日本再生医療学会総会  2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • AhRシグナルの活性化はヒト歯根膜細胞のMMP12発現を亢進する

    門脇正敬、友清淳、糸山知宏、長谷川大学、濱野さゆり、杉井英樹、前田英史

    第158回日本歯科保存学会春季学術大会  2023.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • iPS細胞由来歯根膜幹細胞様細胞の分化誘導におけるFibrillin-2の役割

    濱野さゆり、山下大輝、糸山知宏、友清淳、長谷川大学、杉井英樹、兼子大志、Bara Mardini、前田英史

    第158回日本歯科保存学会春季学術大会  2023.6 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • シュワン細胞はヒト前骨芽細胞の骨芽細胞分化を促進する

    糸山知宏、吉田晋一郎、友清淳、長谷川大学、濱野さゆり、杉井英樹、小野太雅、藤野翔香、一法師啓太、前田英史

    日本歯科保存学会2019年度秋季学術大会  2019.11 

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    Event date： 2019.12

    Language：Japanese  

    Venue：福岡国際会議場（福岡市）   Country：Japan  

  • Activin Aが有する二極性の細胞分化誘導能に関する分子機構の解明

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、Mhd Safwan Albougha、前田英史

    日本歯科保存学会2019年度春季学術大会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：石川県立音楽堂（金沢市）   Country：Japan  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト前骨芽細胞に及ぼす影響について

    吉田晋一郎、糸山知宏、野津葵、杉井英樹、長谷川大学、友清淳、濱野さゆり、前田英史

    日本歯科保存学会2019年度春季学術大会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：石川県立音楽堂（金沢市）   Country：Japan  

  • 穿孔および破折ファイルを有する右側下顎第一大臼歯に対して意図的再植を行った症例

    友清淳、濱野さゆり、長谷川大学、杉井英樹、吉田晋一郎、小野太雅、一法師啓太、山下梢、前田英史

    第40回日本歯内療法学会学術大会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：タワーホール船堀（東京都）   Country：Japan  

  • 次亜塩素酸ナトリウムに浸漬したMineral Trioxide Aggregate が多分化能を有するヒト歯根膜クローン細胞株の石灰化器質形成に及ぼす影響

    山下梢、友清淳、濱野さゆり、長谷川大学、杉井英樹、吉田晋一郎、小野太雅、一法師啓太、前田英史

    第40回日本歯内療法学会学術大会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：タワーホール船堀（東京都）   Country：Japan  

  • TNF-α刺激した老化ヒト歯髄細胞の象牙芽細胞様分化の解析

    野津葵、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、一法師啓太、前田英史

    第42回日本基礎老化学会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：仙台国際センター   Country：Japan  

  • Effects of dopamine on odontoblastic differentiation through PKA signaling.

    Shoko Fujino, Hamano Sayuri, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Ayako Washio, Hiromi Mitarai, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda

    The 97th General Session & Exhibition of the IADR  2019.6 

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    Event date： 2019.12

    Language：English  

    Venue：Vancouver   Country：Canada  

  • Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts.

    Mhd Safwan Albougha, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Taiga Ono, Orie Adachi, Hidefumi Maeda

    日本歯科保存学会2019年度秋季学術大会  2019.11 

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    Event date： 2019.12

    Language：Japanese  

    Venue：福岡国際会議場（福岡市）   Country：Japan  

  • Semaphorin 3Aによる修復象牙質形成過程へのSonic hedgehogシグナルの関与

    吉田晋一郎、糸山知宏、長谷川大学、有馬麻衣、友清淳、濱野さゆり、杉井英樹、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会  2018.11 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都市勧業館みやこめっせ   Country：Japan  

  • GDNFは未分化なヒト歯根膜細胞のシュワン細胞様分化を誘導する

    糸山知宏、吉田晋一郎、友清淳、長谷川大学、濱野さゆり、杉井英樹、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会  2018.11 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都市勧業館みやこめっせ   Country：Japan  

  • Basic Fibroblast Growth FactorおよびephrinB2がヒト歯根膜細胞の増殖に及ぼす影響について

    小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、御手洗裕美、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会  2018.6 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜みなとみらいホール   Country：Japan  

  • 老化したヒト歯髄細胞の象牙芽細胞様分化に及ぼすTNF-αの影響について

    野津葵、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、御手洗裕美、一法師啓太、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会  2018.6 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜みなとみらいホール   Country：Japan  

  • Activin Aがヒト歯根膜細胞およびヒト前骨芽細胞の骨芽細胞様分化に及ぼす影響について

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、御手洗裕美、野津葵、有馬麻衣、糸山知宏、小野太雅、藤野翔香、一法師啓太、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会  2018.6 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜みなとみらいホール   Country：Japan  

  • 象牙芽細胞分化に及ぼすドーパミンの影響について

    藤野翔香、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、鷲尾絢子、御手洗裕美、野津葵、有馬麻衣、和田尚久、北村知昭、前田英史

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福岡国際会議場   Country：Japan  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、野津葵、有馬麻衣、濱野さゆり、御手洗裕美、和田尚久、前田英史

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福岡国際会議場   Country：Japan  

  • ヒト歯髄細胞の象牙芽細胞様分化に及ぼすsFRP1の影響について

    一法師啓太、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会  2018.11 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都市勧業館みやこめっせ   Country：Japan  

  • 未分化なヒト歯根膜クローン細胞株およびハイドロキシアパタイト焼成体を用いた人工歯根作製について

    小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、有馬麻衣、小川真里奈、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会  2018.11 

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    Event date： 2019.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都市勧業館みやこめっせ   Country：Japan  

  • Effects of TNF-a. on Senescent human dental pulp cells.

    Aoi Nozu, Sayuri Hamano ,Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii,Hiromi Mitarai, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda

    Kyudai Oral Bioscience＆OBT Research Center Joint International Symposium 2019  2019.3 

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    Event date： 2019.3

    Language：English  

    Venue：Kyushu University   Country：Japan  

  • Activin A reversely works on the osteoblastic differentiation in human pre-osteoblastic cells and periodontal ligament cells. International conference

    Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Hiromi Mitarai, Aoi Nozu, Mai Arima, Tomohiro Itoyama, Taiga Ono, Shoko Fujino, Keita Ippoushi, Naohisa Wada, Hidefumi Maeda

    The 65th Annual Meeting of Japanese Association for Dental Research  2017.11 

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    Event date： 2018.8

    Language：Japanese  

    Venue：Tokyo   Country：Japan  

  • Sema3Aがヒト歯根膜細胞の幹細胞/未分化細胞誘導に及ぼす影響

    和田 尚久, 前田 英史, 長谷川 大学, 藤井 慎介, 山本 直秀, 友清 淳, 門野内 聡, 赤峰 昭文

    第136回日本歯科保存学会春季学術大会  2012.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • CTGFが未分化なヒト歯根膜細胞株の骨芽細胞様分化に及ぼす影響

    祐田 明香, 前田 英史, 藤井 慎介, 門野内 聡, 山本 直秀, 門野内 聡, 和田 尚久, 友清 淳, 郡 勝明, 河野 清美, 寺松陽子, 濱野 さゆり, 長谷川 大学, 赤峰 昭文

    第137回日本歯科保存学会秋季学術大会  2012.11 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • β2アドレナリン受容体の非作動薬であるPropranololがヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響

    濱野 さゆり, 前田 英史, 友清 淳, 山本 直秀, 門野内 聡, 和田 尚久, 河野 清美, 郡 勝明, 寺松陽子, 長谷川 大学, 祐田 明香, 赤峰 昭文

    第137回日本歯科保存学会秋季学術大会  2012.11 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A

    Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A

    The Australian Health and Medical Research Congress 2012  2012.11 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Conversion of periodontal ligament cells into multipotent stem-like cells

    Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A

    91st General Session & Exhibition of the IADR  2013.3 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Dental pulp stem cells on bone tissue express periodontal ligament-related gene

    Shinichiro Yoshida, Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Hiromi Sato, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine

    The 9th World Endodontic Congress  2013.5 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Activin Aがヒト歯根膜細胞に及ぼす影響について

    杉井 英樹, 前田 英史, 友清 淳, 山本 直秀, 郡 勝明, 和田 尚久, 門野内 聡, 濱野 さゆり, 長谷川 大学, 祐田 明香, 寺松 陽子, 河野 清美, 吉田 晋一郎, 赤峰 昭文

    第138回日本歯科保存学会春季学術大会  2013.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • CTGF/CCN2が未分化なヒト歯根膜細胞株の骨芽細胞様分化に及ぼす影響

    祐田 明香, 前田 英史, 藤井 慎介, 門野内 聡, 山本 直秀, 和田 尚久, 友清 淳, 郡 勝明, 濱野 さゆり, 長谷川 大学, 赤峰 昭文

    第5回CCNファミリー研究会  2013.9 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • β2アドレナリン受容体の作動薬および非作動薬がヒト歯根膜細胞に及ぼす影響

    濱野 さゆり, 前田 英史, 長谷川 大学, 和田 尚久, 友清 淳, 門野内 聡, 祐田 明香, 杉井 英樹, 赤峰昭文

    第140回日本歯科保存学会春季学術大会  2014.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Semaphorin3Aがヒト歯髄幹細胞による硬組織形成に及ぼす影響, 第140回日本歯科保存学会

    吉田 晋一郎, 和田 尚久, 前田 英史, 門野内 聡, 長谷川 大学, 御手洗 裕美, 濱野 さゆり, 祐田 明香, 杉井 英樹, 赤峰 昭文

    第140回日本歯科保存学会春季学術大会  2014.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 骨芽細胞様分化におけるActivin Aの作用は前骨芽細胞と歯根膜細胞とで相反する

    杉井 英樹, 前田 英史, 友清 淳, 和田 尚久, 門野内 聡, 長谷川 大学, 濱野 さゆり, 祐田 明香, 吉田 晋一郎, 赤峰 昭文

    第141回日本歯科保存学会秋季学術大会  2014.10 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohisa Wada, Satoshi Monnouchi, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Akifumi Akamine

    Kyudai Oral Bioscience 2015-9th International Symposium-  2015.2 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Collaborative Activity of CTGF/CCN2 and TGF-β1 on On Osteogenesis and Fibrogenesis International conference

    Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Satoshi Monnouchi, Naohisa Wada, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine

    93rd General Session & Exhibition of the IADR  2015.3 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Effects of β2 Adrenergic Receptor oh Human Periodontal Ligament Cells International conference

    Sayuri Hamano, Hidefumi Maeda, Daigaku Hasegawa, Satoshi Monnouchi, Naohisa Wada, Atsushi Tomokiyo, Asuka Yuda, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine

    93rd General Session & Exhibition of the IADR  2015.3 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 歯根膜および皮膚由来ヒト人工多能性幹細胞（iPSC）を用いた神経堤細胞様細胞の樹立とその表現型の比較

    友清 淳, 前田 英史, 和田 尚久, 門野内 聡, 濱野 さゆり, 長谷川 大学, 祐田 明香, 赤峰 昭文

    第142回日本歯科保存学会春季学術大会  2015.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 歯根膜細胞におけるα-SMA発現にTransgelinが関与する

    御手洗 裕美, 和田 尚久, 前田 英史, 長谷川 大学, 吉田 晋一郎, 濱野 さゆり, 祐田 明香, 友清 淳, 赤峰 昭文

    第142回日本歯科保存学会春季学術大会  2015.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Generation of neural crest-like cells from human periodontal ligament cell-derived induced pluripotent stem cells

    Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda

    63rd Annual Meeting of Japanese Association for Dental research  2015.10 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • ヒト歯髄細胞の石灰化におけるCalcium-sensing receptorの関与について

    水町 博之, 友清 淳, 長谷川 大学, 濱野 さゆり, 吉田 晋一郎, 杉井 英樹, 芹田 俊, 御手洗 裕美, 和田 尚久, 前田 英史

    第143回日本歯科保存学会秋季学術大会  2015.11 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 炎症性サイトカインで刺激したヒト歯根膜細胞由来のGDNFはPC12の神経細胞分化を促進する

    吉田 晋一郎, 山本 直秀, 和田 尚久, 友清 淳, 長谷川 大学, 濱野 さゆり, 祐田 明香, 御手洗 裕美, 杉井 英樹, 前田 英史

    第143回日本歯科保存学会秋季学術大会  2015.11 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Activin A reversely works between human pre-osteoblastic cells and periodontal ligament cells on their osteoblastic differentiation International conference

    Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Aoi Nozu, Marina Ogawa, Mai Sonoda, Naohisa Wada, Hidefumi Maeda

    2nd symposium of Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers  2016.2 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells International conference

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda

    Kyudai Oral Bioscience 2016 –Frontiers in Dental Research and Education in East Asia-  2016.2 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Directing human iPS cells toward PDL stem cells International conference

    Sayuri Hamano, Atsushi Tomokiyo, Naohisa Wada, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Hidefumi Maeda

    94th General Session & Exhibition of the IADR  2016.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells International conference

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda

    94th General Session & Exhibition of the IADR  2016.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 歯髄細胞におけるβig-h3の発現および機能について

    芹田 俊, 友清 淳, 長谷川 大学, 濱野 さゆり, 杉井 英樹, 吉田 晋一郎, 水町 博之, 御手洗 裕美, 和田 尚久, 前田 英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • iPS細胞由来の歯根膜幹細胞様細胞の樹立

    濱野 さゆり, 友清 淳, 和田 尚久, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 芹田 俊, 水町 博之, 御手洗 裕美, 前田 英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • ヒト歯髄細胞の老化と象牙芽細胞様分化に及ぼすTNF-αの影響について

    野津 葵, 友清 淳, 長谷川 大学, 濱野 さゆり, 吉田 晋一郎, 芹田 俊, 御手洗 裕美, 和田 尚久, 前田 英史

    第37回日本歯内療法学会  2016.7 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 歯根膜および皮膚由来ヒト人工多能性幹細胞（iPSC）を用いた神経堤細胞様細胞の樹立とキャラクタリゼーション

    友清 淳, 和田 尚久, 長谷川 大学, 濱野 さゆり, 杉井 英樹, 吉田 晋一郎, 前田 英史

    第23回日本歯科医学会総会  2016.10 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 根管治療ならびに修復処置関連溶液によって誘導されるMineral Trioxide Aggregateの色調変化に関する比較分析

    友清 淳, 和田 尚久, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 芹田 俊, 御手洗 裕美, 水町 博之, 前田 英史

    第145回日本歯科保存学会秋季学術大会  2016.10 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Transgelin Mediates TGF-beta1-induced Human Periodontal Ligament Cell Proliferation International conference

    Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

    95th General Session & Exhibition of the IADR  2017.3 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • 新規象牙芽細胞マーカーとしてのチロシン水酸化酵素の可能性

    藤野 翔香, 濱野 さゆり, 芹田 俊, 友清 淳, 長谷川 大学, 吉田 晋一郎, 水町 博之, 御手洗 裕美, 和田 尚久, 清島 保, 前田 英史

    第146回日本歯科保存学会春季学術大会  2017.6 

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    Event date： 2018.8

    Language：Japanese  

    Country：Japan  

  • Nano Hydoxyapatite含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、御手洗裕美、有馬麻衣、濱野さゆり、野津葵、和田尚久、前田英史

    第147回日本歯科保存学会秋季学術大会  2017.10 

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    Event date： 2018.8

    Language：Japanese  

    Venue：マリオス（盛岡市）   Country：Japan  

  • Superoxide Dismutase 2の発現抑制による歯髄細胞の細胞老化が石灰化誘導能に及ぼす影響

    糸山知宏、虎谷渓、門脇正敬、吉田晋一郎、長谷川大学、杉井英樹、兼子大志、前田英史

    第45回日本歯内療法学会学術大会  2024.7 

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    Presentation type：Oral presentation (general)  

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MISC

  • Current Application of iPS Cells in the Dental Tissue Regeneration Reviewed

    Hamano S, Sugiura R, Yamashita D, Tomokiyo A, Hasegawa D, Maeda H

    Biomedicines   2022.12

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.3390/biomedicines10123269

  • Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy Reviewed

    Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Maeda H

    Biology (Basel)   2020.7

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Detection, characterization, and clinical application of mesenchymal stem cells in periodontal ligament tissue

    Tomokiyo, A., Yoshida, S., Hamano, S., Hasegawa, D., Sugii, H. & Maeda, H.

    Stem Cells International.   2018.8

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues

    Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda

    Journal of Stem Cell Research & Therapeutics   2016.7

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Professional Memberships

  • 日本歯科保存学会

  • 日本歯内療法学会

  • 日本再生医療学会

  • International Association of Dental Research

  • 日本軟骨代謝学会

  • 日本骨代謝学会

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Committee Memberships

  • 日本歯科保存学会   Steering committee member   Domestic

    2023.4 - Present   

  • 日本歯科保存学会   編集委員会委員   Domestic

    2023.4 - Present   

Academic Activities

  • コメンテーター

    第22回日本再生医療学会  （ Japan ） 2023.3

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    Type：Competition, symposium, etc. 

  • 大会準備委員長

    第151回日本歯科保存学会  （ Japan ） 2019.11

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    Type：Competition, symposium, etc. 

Research Projects

  • 歯根膜幹細胞転換を応用した革新的歯周組織再生法の創出

    2024.4 - 2027.3

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    Authorship：Principal investigator 

  • 歯根膜幹細胞転換を応用した革新的歯周組織再生法の創出

    Grant number：24K12948  2024 - 2026

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 歯根膜幹細胞転換を誘導する化合物の探索およびそれを用いた新規歯周組織再生法の創出

    2022.4 - 2024.3

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    Authorship：Principal investigator 

  • 歯根膜幹細胞転換を誘導する化合物の探索およびそれを用いた新規歯周組織再生法の創出

    Grant number：22K21018  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity start-up

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 頭蓋冠の発達と疾患を制御する骨格形成シグナルの解明 International coauthorship

    2020.10 - 2022.3

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    Authorship：Principal investigator 

  • 2020年度上原記念生命科学海外留学助成リサーチフェローシップ/頭蓋冠の発達と疾患を制御する骨格形成シグナルの解明

    2020

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    Grant type：Donation

  • 歯根膜幹細胞転換を基盤とした歯周組織再生の有用性の検討とそのメカニズムの解明

    2019.4 - 2020.9

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    Authorship：Principal investigator 

  • 歯根膜幹細胞転換を基盤とした歯周組織再生の有用性の検討とそのメカニズムの解明

    Grant number：19K19002  2019 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Early-Career Scientists

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • iPS創薬とゲノム創薬の融合による歯根膜幹細胞賦活化物質の創出と再生医療への応用

    2018.4 - 2021.3

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    Authorship：Coinvestigator(s) 

  • 誘導性ヒト歯根膜幹細胞を活用した新規歯周組織再生療法の開発

    2018.4 - 2019.3

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    Authorship：Principal investigator 

  • iPS創薬とゲノム創薬の融合による歯根膜幹細胞賦活化物質の創出と再生医療への応用

    Grant number：18K19651  2018 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 誘導性ヒト歯根膜幹細胞を活用した新規歯周組織再生療法の開発

    2018

    平成30年度QRプログラム・わかばチャレンジ

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

  • iPS細胞を用いた3次元的歯根膜作製法の確立と人工歯根開発への応用

    2017.4 - 2022.3

  • iPS由来神経堤細胞によるバイオミメティクスに基づいた新規歯周組織再生療法の開発

    2017.4 - 2021.3

  • iPS細胞を用いた3次元的歯根膜作製方法の確立と人工歯根開発への応用

    Grant number：17H01598  2017 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • iPS由来神経堤細胞によるバイオミメティクスに基づいた新規歯周組織再生療法の開発

    Grant number：17H04385  2017 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • Wnt5aシグナルによる効果的な歯根膜組織再生を用いた次世代型意図的再植法の開発

    2016.4 - 2018.3

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    Authorship：Principal investigator 

  • Wnt5aシグナルによる効果的な歯根膜組織再生を用いた次世代型意図的再植法の開発

    Grant number：16K20458  2016 - 2017

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • Wnt5aによる歯根膜線維組織再生誘導を基盤とした改良型意図的再植法の開発

    2015.4 - 2016.3

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    Authorship：Principal investigator 

  • Wnt5aによる歯根膜線維組織再生誘導を基盤とした改良型意図的再植法の開発

    Grant number：15H06487  2015 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity start-up

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 新規の歯根膜幹細胞表面抗原および歯根膜マーカーを活用した歯周組織再生法の開発

    2011.4 - 2014.3

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Educational Activities

  • ・歯学部4年生に対する講義
    ・歯学部5年生に対する臨床予備実習
    ・歯学部5，6年生に対する病院臨床実習
    ・初期研修医および後期研修医に対する臨床指導
    ・大学院生に対する研究および臨床指導
    ・社会人大学院生に対する研究指導
    ・シリア人および中国人留学生に対する研究指導

Class subject

  • 歯科保存学Ⅰ

    2025.4 - 2025.9   First semester

  • 歯科保存学Ⅰ

    2024.4 - 2024.9   First semester

  • 歯科保存学Ⅰ

    2023.4 - 2023.9   First semester

  • 歯科保存学Ⅰ

    2022.4 - 2022.9   First semester

FD Participation

  • 2025.6   Title：WHO口腔保健プログラムでの仕事：ユニバーサルヘルスガバレッジ達成に向けて

  • 2025.6   Title：歯科における重症偶発症への対策と対応

  • 2023.6   Title：科学研究費補助金採択率向上に向けた工夫

  • 2019.10   Role：Speech   Title：令和元年度馬出地区４部局合同男女共同参画FD

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2018.3   Title：東京医歯大における国家試験対策の実践

  • 2017.11   Title：ARO次世代医療センターの活用方法

  • 2016.12   Title：反グローバル化と国際交流

  • 2016.12   Title：歯学教育の現状と課題

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Outline of Social Contribution and International Cooperation activities

  • ・当科受診患者に対しメンテナンス・リコールを行うことで、口腔ケアの重要性の啓発に寄与している。
    ・CR修復に関する症例発表会において発表するなど、大学病院と開業医との臨床連携活動に貢献している。
    ・シリア人および中国人留学生に対し研究指導・共同研究を行うことで、学術的国際交流の発展に寄与している。

Travel Abroad

  • 2021.6 - 2022.3

    Staying countory name 1：United States   Staying institution name 1：Forsyth Institute

  • 2020.10 - 2021.5

    Staying countory name 1：United States   Staying institution name 1：University of Rochester medical Center

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

Clinician qualification

  • Certifying physician

    日本歯科保存学会

Year of medical license acquisition

  • 2010

Notable Clinical Activities

  • ・難治性の根尖性歯周炎に対して、歯科用顕微鏡（マイクロスコープ）やCBCT等の機材を用いた高度先進歯内治療を行い、歯の可及的保存を図っている。 ・歯根端切除術等の外科的歯内療法を行うことで、通常の根管治療では治癒しきらなかった歯を治癒へと導いている。 ・通常抜歯適応と診断されている歯根破折歯に対して、ファイバーポストを併用した接着システムを活用した意図的再植術を行い、歯の保存適応範囲の拡大を図っている。 ・開業医において対応困難とされる、破折した根管治療用器具の除去を行っている。 ・外傷等による前歯部歯冠破折症例に対し、審美性に配慮したコンポジットレジン修復を行っている。