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Taichi Itoh Last modified date:2021.12.21

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Doctor of Science
Country of degree conferring institution (Overseas)
Field of Specialization
Total Priod of education and research career in the foreign country
Outline Activities
Comprehensive analysis of the sleep regulation
Research Interests
  • Behavioral analysis of Hydra
    keyword : Hydra, Nerve nets, clock genes
  • Identifying new clock genes in Drosophila
    keyword : Clock genes Drosophila melanogaster
Academic Activities
1. Itoh T.Q., Matsumoto A., Self Assembly (SA)-cloning: A rapid construction method for recombinant molecules from multiple fragments., BioTechniques Protocol Guide 2012, 2011.12.
1. Tomas S. Andreani, Taichi Q. Itoh, Evrim Yildirim, Dae-Sung Hwangbo, Ravi Allada, Genetics of Circadian Rhythms, 10.1016/j.jsmc.2015.08.007, Vol.10, No.4, pp.413-421, 2015.12.
2. ショウジョウバエを用いた概日測時機構解析の40年―第2部 ショウジョウバエ再び―.
3. ショウジョウバエを用いた概日測時機構解析の40年―第1部 ショウジョウバエは再びとべるか?―.
1. Hiroyuki J. Kanaya, Sungeon Park, Ji-hyung Kim, Junko Kusumi, Sofian Krenenou, Etsuko Sawatari, Aya Sato, Jongbin Lee, Hyunwoo Bang, Yoshitaka Kobayakawa, Chunghun Lim, Taichi Q. Itoh, A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system, Science Advances, 10.1126/sciadv.abb9415, 6, 41, eabb9415-eabb9415, 2020.10, Sleep behaviors are observed even in nematodes and arthropods, yet little is known about how sleep-regulatory mechanisms have emerged during evolution. Here, we report a sleep-like state in the cnidarian Hydra vulgaris with a primitive nervous organization. Hydra sleep was shaped by homeostasis and necessary for cell proliferation, but it lacked free-running circadian rhythms. Instead, we detected 4-hour rhythms that might be generated by ultradian oscillators underlying Hydra sleep. Microarray analysis in sleep-deprived Hydra revealed sleep-dependent expression of 212 genes, including cGMP-dependent protein kinase 1 (PRKG1) and ornithine aminotransferase. Sleep-promoting effects of melatonin, GABA, and PRKG1 were conserved in Hydra. However, arousing dopamine unexpectedly induced Hydra sleep. Opposing effects of ornithine metabolism on sleep were also evident between Hydra and Drosophila, suggesting the evolutionary switch of their sleep-regulatory functions. Thus, sleep-relevant physiology and sleep-regulatory components may have already been acquired at molecular levels in a brain-less metazoan phylum and reprogrammed accordingly..
2. Hiroyuki J. Kanaya, Yoshitaka Kobayakawa, Taichi Q. Itoh, Hydra vulgaris exhibits day-night variation in behavior and gene expression levels, Zoological Letters, 10.1186/s40851-019-0127-1, 5, 1, 2019.12.
3. Shun Uchizono, Yumi Tabuki, Natsumi Kawaguchi, Teiichi Tanimura, Taichi Q. Itoh, Mated Drosophila melanogaster females consume more amino acids during the dark phase, PLOS ONE, 10.1371/journal.pone.0172886, 12, 2, e0172886-e0172886, 2017.02.
4. Taichi Q. Itoh, Teiichi Tanimura, Akira Matsumoto, Membrane-bound transporter controls the circadian transcription of clock genes in Drosophila, Genes to Cells, 10.1111/j.1365-2443.2011.01559.x, 16, 12, 1159-1167, 2011.12.
5. Akira Matsumoto, Taichi Q. Itoh, Self-assembly cloning: a rapid construction method for recombinant molecules from multiple fragments, BioTechniques, 10.2144/000113705, 51, 1, 55-56, 2011.07, Enzyme-free cloning (EFC) can rapidly produce an in-frame fusion gene with multiple fragments. To practically apply EFC, we investigated the extent and sequence of complementary staggered overhangs necessary to direct self-assembly of multiple fragments as well as a size limitation of the constructed DNA molecule. Six-base pair overhangs with 50% GC content were sufficient to direct self-assembly. A functional plasmid that exceeded 10 kb, which includes an in-frame fusion domain, was efficiently constructed from four PCR fragments in one step by our improved method..
1. T.Q. Itoh, A sleep-like state in Hydra unravels conserved sleep mechanisms., SLEEP 2021, the 35th annual meeting of the APSS, 2021.06.
2. T.Q. Itoh, Circadian and sleep behavior in clockless and brainless animal Hydra., The 2nd Korean Biological Rhythm Society Symposium, 2021.02.
Membership in Academic Society