| 1. |
Sugii H, Albougha MS, Adachi O, Tomita Y, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Itoyama T, Maeda H., Activin A Promotes Osteoblastic Differentiation of Human Preosteoblasts through the ALK1-Smad1/5/9 Pathway, International journal of molecular sciences, 2021.12. |
| 2. |
Kaneko H, Hasegawa D, Itoyama T, Yoshida S, Tomokiyo A, Hamano S, Sugii H, Maeda H., Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues, Archives o Oral Biology, 2022.02. |
| 3. |
Yamashita K, Tomokiyo A, Ono T, Ipposhi K, Anas MA, Tsuchiya A, Hamano S, Sugii H, Yoshida S, Itoyama T, Maeda H, Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells., Scientific Reports, 2021.11. |
| 4. |
Yoshida S, Sugii H, Itoyama T, Kadowaki M, Hasegawa D, Tomokiyo A, Hamano S, Ipposhi K, Yamahita K, Maeda H., Development of a novel direct pulp-capping material using 4-META/MA-TBB resin with nanohydroxyapatite., Materials Science and Engineering: C, 10.1016/j.msec.2021.112426, 2021.11. |
| 5. |
Ipposhi K, Tomokiyo A, Ono T, Yamashita K, Anas MA, Hasegawa D, Hamano S, Yoshida S, Sugii H, Itoyama T, Ogawa M, Maeda H, Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells., Cells, 10.3390/cells10092491, 10, 9, 2021.09. |
| 6. |
Hasegawa D, Hasegawa K, Kaneko H, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Wada N, Kiyoshima T, Maeda H, MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells. , Stem cells international, 2020.07. |
| 7. |
Tomohiro Itoyama, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Taiga Ono, Shoko Fujino, Hidefumi Maeda, Possible function of GDNF and Schwann cells in wound healing of periodontal tissue., Journal of Periodontal Research, 2020.06. |
| 8. |
Hamano S, Tomokiyo A, Hasegawa D, Yuda A, Sugii H, Yoshida S, Mitarai H, Wada N,Maeda H., Functions of beta2-adrenergic receptor in human periodontal ligament cells., Journal of cellular biochemistry, 2020.03. |
| 9. |
Fujino S, Hamano S, Tomokiyo A, Itoyama T, Hasegawa D, Sugii H, Yoshida S, Washio A, Nozu A, Ono T, Wada N, Kitamura C, Maeda H., Expression and function of dopamine in odontoblasts., Journal od Cellular Physiology, 2019.10. |
| 10. |
Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiy A, Hamano S, Sugii H, Wada N, Maeda H., R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway., Journal of Periodontal Research, 2018.04. |
| 11. |
Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H., Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue., Stem Cells Intrnational, 2018.08. |
| 12. |
Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H, Senescence and odontoblastic differentiation of dental pulp cells., Journal of cellular physiology, 2018.01. |
| 13. |
Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H., Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells., Stem cells and development., 2017.12. |
| 14. |
Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda, Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling., Journal of Cellular Physiology, 2017.08. |
| 15. |
Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda, Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells., Bone., 2017.08. |
| 16. |
Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hiroyuki Mizumachi, Hidefumi Maeda, Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells., Journal of Periodontal research., 2017.06. |
| 17. |
Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Hiroyuki Mizumachi, Hiromi Mitarai, Satoshi Monnouchi, Naohisa Wada, Hidefumi Maeda, Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells., Archives of oral biology., 2017.06. |
| 18. |
Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda, Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues., Journal of Stem Cell Research & Therapy, 2016.07. |
| 19. |
Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Satoshi Monnouchi, Asuka Yuda, Hidefumi Maeda, GDNF from Human Periodontal Ligament Cells Treated with Proinflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells., Journal of Cellular Biochemistry, 2016.07, Glial cell line-derived neurotrophic factor (GDNF) is known to mediate multiple biological activities such as promotion of cell motility and proliferation, and morphogenesis. However, little is known about its effects on periodontal ligament (PDL) cells. Recently, we reported that GDNF expression is increased in wounded rat PDL tissue and human PDL cells (HPDLCs) treated with proinflammatory cytokines. Here, we investigated the associated expression of GDNF and the proinflammatory cytokine interleukin-1 beta (IL-1β) in wounded PDL tissue, and whether HPDLCs secrete GDNF which affects neurocytic differentiation. Rat PDL cells near the wounded area showed intense immunoreactions against an anti-GDNF antibody, where immunoreactivity was also increased against an anti-IL-1β antibody. Compared with untreated cells, HPDLCs treated with IL-1β or tumor necrosis factor-alpha showed an increase in the secretion of GDNF protein. Conditioned medium of IL-1β-treated HPDLCs (IL-1β-CM) increased neurite outgrowth of PC12 rat adrenal pheochromocytoma cells. The expression levels of two neural regeneration-associated genes, growth-associated protein-43 (Gap-43) and small proline-rich repeat protein 1A (Sprr1A), were also upregulated in IL-1β-CM-treated PC12 cells. These stimulatory effects of IL-1β-CM were significantly inhibited by a neutralizing antibody against GDNF. In addition, U0126, a MEK inhibitor, inhibited GDNF-induced neurite outgrowth of PC12 cells. These findings suggest that an increase of GDNF in wounded PDL tissue might play an important role in neural regeneration probably via the MEK/ERK signaling pathway.. |
| 20. |
Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Hirofumi Miyaji, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda, Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells, Journal of Dental Research, 2016.06, In cases of pulp exposure due to deep dental caries or severe traumatic injuries, existing pulp-capping materials have a limited ability to reconstruct dentin-pulp complexes and can result in pulpectomy because of their low potentials to accelerate dental pulp cell activities, such as migration, proliferation, and differentiation. Therefore, the development of more effective therapeutic agents has been anticipated for direct pulp capping. Dental pulp tissues are enriched with dental pulp stem cells (DPSCs). Here, the authors investigated the effects of semaphorin 3A (Sema3A) on various functions of human DPSCs in vitro and reparative dentin formation in vivo in a rat dental pulp exposure model. Immunofluorescence staining revealed expression of Sema3A and its receptor Nrp1 (neuropilin 1) in rat dental pulp tissue and human DPSC clones. Sema3A induced cell migration, chemotaxis, proliferation, and odontoblastic differentiation of DPSC clones. In addition, Sema3A treatment of DPSC clones increased β-catenin nuclear accumulation, upregulated expression of the FARP2 gene (FERM, RhoGEF, and pleckstrin domain protein 2), and activated Rac1 in DPSC clones. Furthermore, in the rat dental pulp exposure model, Sema3A promoted reparative dentin formation with dentin tubules and a well-aligned odontoblast-like cell layer at the dental pulp exposure site and with novel reparative dentin almost completely covering pulp tissue at 4 wk after direct pulp capping. These findings suggest that Sema3A could play an important role in dentin regeneration via canonical Wnt/β-catenin signaling. Sema3A might be an alternative agent for direct pulp capping, which requires further study.. |
| 21. |
Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Satoshi Monnouchi, Sayuri Hamano, Asuka Yuda, Akifumi Akamine, Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through TGFβ1-mediated Upregulation of Periostin Expression., Journal of Cellular Physiology, 2015.11. |
| 22. |
Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P. Mark Bartold, Danijela Menicanin, Fujii Shinsuke, Shinichiro Yoshida, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine, Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells., Stem Cells and Development, 2014.09. |
