| TSE KA FAI WILLIAM | Last modified date：2024.06.03 |
Associate Professor /
Attached Promotive Center for International Education and Research of Agriculture
Faculty of Agriculture
Faculty of Agriculture
Undergraduate School
Other Organization
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Homepage
https://kyushu-u.elsevierpure.com/en/persons/ka-fai-william-tse
Reseacher Profiling Tool Kyushu University Pure
Phone
092-642-7046
Fax
092-642-2804
Academic Degree
Ph.D; Hong Kong Baptist University (Hong Kong S.A.R.)
Country of degree conferring institution (Overseas)
Yes Bachelor Doctor
Field of Specialization
Developmental Biology; Fish Osmoregulation; Experimental pathology
ORCID(Open Researcher and Contributor ID)
0000-0002-3738-0460
Total Priod of education and research career in the foreign country
08years00months
Outline Activities
Research:
We have four major research themes:
1) Developmental functions of deubiquitylating enzyme: Deconjugation of ubiquitin and/or ubiquitin-like modified protein substrates is essential to modulate protein-protein interactions and, thus, signaling processes in cells. Although deubiquitylating (deubiquitinating) enzymes (DUBs) play a key role in this process, however, their function and regulation remain insufficiently understood. Our group performed the first genome-wide DUBs loss-of-function analysis in zebrafish, and we are now undergoing different experiments on selected DUBs to unfold their regulatory mechanisms and specific developmental functions. In 2013, we have identified that zebrafish transforming growth factor-β-stimulated clone 22 domain 3 (Tsc22d3) could function with two DUBs via the bone morphogenetic protein (bmp) pathway (Tse et al., 2013a). We are now working on the biochemical mechanism of the selected DUBs.
2) Disease model and mechanism: Zebrafish have been widely used in developmental biology, their well-established genome database and transparent embryos shaped them as an excellent developmental model. Recently, they are recruited as a new disease model. The zebrafish genome project identified that over 70% human disease causing genes can be found in the zebrafish genome. We own several organ-specific GFP-lines and mutants for different disease-based studies. Our group has a special interest on the mechanism of cleft lip and/or palate (CLP). CLP is one of the most common birth defects in the world, with an average frequency of 1/700. CLP besides affecting the appearance also causes significant morbidity in form and function. We selected several potential human CLP causing genes to study their functional roles in the facial development, which aim to understand the disease mechanism and thus identify small molecules to treat the disease before birth. Recently, we have identified the pathogenesis of the Type 3 Treacher Collins Syndrome by using a zebrafish model (Lau et al. 2016).
3) Osmoregulation: The capability of animal cells to maintain a constant cell volume is a prerequisite for cellular life. When eukaryotic cells are exposed to extracellular osmotic stress, they undergo rapid regulatory processes to maintain their cellular homeostatic status. The mechanism is particularly important in gill epithelia in fishes. Recently, our group applied the next-generation sequencing (NGS) and proteomics technologies to report the first eel gill specific transcriptome data and osmo-responsive proteins in eel gill (Tse et al. 2013b; Tse et al. 2014; Lai et al. 2015). Furthermore, our group use different fish models (eel, medaka, and zebrafish) to understand the molecular issues in the osmoregulation process (Chow et al. 2013; Lai et al. 2013; Tse et al. 2013c).
4) Developmental toxicity: Our group recently starts to apply developmental biology to environmental toxicology studies. Effects of toxicants on embryogenesis can be easily being tested in zebrafish embryos in a large scale. We have found that the exposure of environmental pollutant bisphenol A (BPA) and Triclosan could influence zebrafish embryos’ early development (Tse et al. 2013d) or lipid metabolism (Ho et al. 2016), respectively. Our group is now testing some other pollutants on their potential hazards on embryonic development.
Education:
I have teaching duties in the English-taught International undergraduate program, and am involved in the "Global Human Resource Development Project" (Hong Kong region).
We have four major research themes:
1) Developmental functions of deubiquitylating enzyme: Deconjugation of ubiquitin and/or ubiquitin-like modified protein substrates is essential to modulate protein-protein interactions and, thus, signaling processes in cells. Although deubiquitylating (deubiquitinating) enzymes (DUBs) play a key role in this process, however, their function and regulation remain insufficiently understood. Our group performed the first genome-wide DUBs loss-of-function analysis in zebrafish, and we are now undergoing different experiments on selected DUBs to unfold their regulatory mechanisms and specific developmental functions. In 2013, we have identified that zebrafish transforming growth factor-β-stimulated clone 22 domain 3 (Tsc22d3) could function with two DUBs via the bone morphogenetic protein (bmp) pathway (Tse et al., 2013a). We are now working on the biochemical mechanism of the selected DUBs.
2) Disease model and mechanism: Zebrafish have been widely used in developmental biology, their well-established genome database and transparent embryos shaped them as an excellent developmental model. Recently, they are recruited as a new disease model. The zebrafish genome project identified that over 70% human disease causing genes can be found in the zebrafish genome. We own several organ-specific GFP-lines and mutants for different disease-based studies. Our group has a special interest on the mechanism of cleft lip and/or palate (CLP). CLP is one of the most common birth defects in the world, with an average frequency of 1/700. CLP besides affecting the appearance also causes significant morbidity in form and function. We selected several potential human CLP causing genes to study their functional roles in the facial development, which aim to understand the disease mechanism and thus identify small molecules to treat the disease before birth. Recently, we have identified the pathogenesis of the Type 3 Treacher Collins Syndrome by using a zebrafish model (Lau et al. 2016).
3) Osmoregulation: The capability of animal cells to maintain a constant cell volume is a prerequisite for cellular life. When eukaryotic cells are exposed to extracellular osmotic stress, they undergo rapid regulatory processes to maintain their cellular homeostatic status. The mechanism is particularly important in gill epithelia in fishes. Recently, our group applied the next-generation sequencing (NGS) and proteomics technologies to report the first eel gill specific transcriptome data and osmo-responsive proteins in eel gill (Tse et al. 2013b; Tse et al. 2014; Lai et al. 2015). Furthermore, our group use different fish models (eel, medaka, and zebrafish) to understand the molecular issues in the osmoregulation process (Chow et al. 2013; Lai et al. 2013; Tse et al. 2013c).
4) Developmental toxicity: Our group recently starts to apply developmental biology to environmental toxicology studies. Effects of toxicants on embryogenesis can be easily being tested in zebrafish embryos in a large scale. We have found that the exposure of environmental pollutant bisphenol A (BPA) and Triclosan could influence zebrafish embryos’ early development (Tse et al. 2013d) or lipid metabolism (Ho et al. 2016), respectively. Our group is now testing some other pollutants on their potential hazards on embryonic development.
Education:
I have teaching duties in the English-taught International undergraduate program, and am involved in the "Global Human Resource Development Project" (Hong Kong region).
Research
Research Interests
- Rare disease model; Environmental toxicant screening; Developmental Toxicology; Integrated omics
keyword : Zebrafish; Disease model; Rare diseases; Craniofacial Developmental Biology; Signaling pathways; Experimental pathology; Environmental Sciences; Pollutant; Developmental toxicology; PFAS; Fish osmoregulation; Osmotic stress; Gill; Gut; Medaka
2006.09～2034.02. - disease pathogenesis
keyword : zebrafish; disease model; disease mechanism
2013.09. - developmental toxicology
keyword : zebrafish; environmental pollutants
2013.09. - osmoregulation
keyword : fish; gill; cellular volume; ion transporters; cytoskeleton
2006.09.
Papers
Educational
Educational Activities
I am involved in five courses in the IUP:
Applied Cell Biology
Bioresource and Bioenvironment Experiments and Practice 1
Fundamental Cell Biology
Physiology
Special Lecture on Advanced Topics of Agriculture 1
Applied Cell Biology
Bioresource and Bioenvironment Experiments and Practice 1
Fundamental Cell Biology
Physiology
Special Lecture on Advanced Topics of Agriculture 1
Social
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