Kyushu University Academic Staff Educational and Research Activities Database
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Yukiko Ogino Last modified date:2023.06.20

Associate Professor / Animal and Marine Bioscience Educational Course, Graduate School pf Bioresource and Bioenvironment Sciences
Attached Promotive Center for International Education and Research of Agriculture
Faculty of Agriculture

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 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Field of Specialization
Endocrinology, Evolutionary Biology, Environmental Science
Outline Activities
Education: I am in charge of the classes regarding Ecology and Evolutionary Biology in IUPE general education courses.
Research: My research is focused on the molecular basis of androgen-dependent sex characteristics development and evolution, which brings the morphological specification of male-type sex attractive and reproductive organs, and the wide variety of reproductive strategies in vertebrates.
Research Interests
  • Sex spectrum of the secondary sex characteristics : Regulation of size and tissue specificity of sex characteristics by androgen
    keyword : tissue specificity, sex characteristics, chromatin, androgen effector gene
  • Mechanisms of diversification of sex characteristics: a new insight from the functional analysis of androgen receptor genes
    keyword : Androgen receptor, medaka
  • Analysis of chromatin dynamics during the sex characteristics development
    keyword : androgen receptor
  • Analysis of the relation between sex characteristics development and the individual recognition, the emotional control system using fish as model animal
    keyword : environment, endocrine system, stress
  • Molecular basis of androgen-dependent sex characteristics development and evolution
    Generation of bio-monitoring medaka
    keyword : androgen, sex characteristics, morphological diversification, genome duplication, endocrine disruptors
Academic Activities
1. Yukiko Ogino, Saki Tohyama, Satomi Kohno, Kenji Toyota, Gen Yamada, Ryohei Yatsu, Tohru Kobayashi, Norihisa Tatarazako, Tomomi Sato, Hajime Matsubara, Anke Lange, Charles R. Tyler, Yoshinao Katsu, Taisen Iguchi, Shinichi Miyagawa, Functional distinctions associated with the diversity of sex steroid hormone receptors ESR and AR, Journal of Steroid Biochemistry and Molecular Biology, 10.1016/j.jsbmb.2018.06.002, 2018.11, Sex steroid hormones including estrogens and androgens play fundamental roles in regulating reproductive activities and they act through estrogen and androgen receptors (ESR and AR). These steroid receptors have evolved from a common ancestor in association with several gene duplications. In most vertebrates, this has resulted in two ESR subtypes (ESR1 and ESR2) and one AR, whereas in teleost fish there are at least three ESRs (ESR1, ESR2a and ESR2b) and two ARs (ARα and ARβ) due to a lineage-specific whole genome duplication. Functional distinctions have been suggested among these receptors, but to date their roles have only been characterized in a limited number of species. Sexual differentiation and the development of reproductive organs are indispensable for all animal species and in vertebrates these events depend on the action of sex steroid hormones. Here we review the recent progress in understanding of the functions of the ESRs and ARs in the development and expression of sexually dimorphic characteristics associated with steroid hormone signaling in vertebrates, with representative fish, amphibians, reptiles, birds and mammals..
1. Yukiko Ogino1,2*, Satoshi Ansai3,4, Eiji Watanabe5,6, Masaki Yasugi7,8, Yukitoshi Katayama9, Hirotaka Sakamoto9, Keigo Okamoto1, Kataaki Okubo10, Yasuhiro Yamamoto11, Ikuyo Hara12,13, Touko Yamazaki12, Ai Kato13, Yasuhiro Kamei6,14, Kiyoshi Naruse6,12,13, Kohei Ohta15, Hajime Ogino16, Tatsuya Sakamoto9, Shinichi Miyagawa17, Tomomi Sato18, Gen Yamada19, Michael E. Baker20, Taisen Iguchi18, Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish, Nat Commun,, 14, 1, article NO.1428, 2023.03, Teleost fishes exhibit complex sexual characteristics, such as fin enlargement and courtship display, in response to androgens. However, the molecular mechanisms underlying their evolutionary acquisition remain largely unknown. To address this question, we analysed medaka (Oryzias latipes) mutants deficient in teleost-specific androgen receptor ohnologs (ara and arb). We discovered that neither ar ohnolog was required for spermatogenesis, whilst they appear to be functionally redundant for the courtship display in males. However, both were required for reproductive success: ara for tooth enlargement and the reproductive behaviour eliciting female receptivity, arb for male-specific fin morphogenesis and sexual motivation. We further showed that differences between the two ars in their transcription, cellular localisation of their encoded proteins, and their downstream genetic programs could be responsible for the phenotypic diversity between the ara and arb mutants. These findings suggest that the ar ohnologs have diverged in two ways: first through the loss of their roles in spermatogenesis, and second, through the gene duplication followed by functional differentiation that has likely resolved the pleiotropic roles derived from their ancestral gene. Thus, our results provide insights into how genome duplication impacts the massive diversification of sexual characteristics in the teleost lineage..
2. Yukiko Ogino, Shigehiro Kuraku, Hiroshi Ishibashi, Hitoshi Miyakawa, Eri Sumiya, Shinichi Miyagawa, Hajime Matsubara, Gen yamada, Michael E. Baker, Taisen Iguchi, Neofunctionalization of androgen receptor by gain-of-function mutations in teleost fish lineage, Molecular Biology and Evolution, 33, 228-244, 2016.01.
3. Yukiko Ogino, Ikumi Hirakawa, Keiji Inohaya, Eri Sumiya, Shinichi Miyagawa, Nancy Denslow, Gen Yamada, Norihisa Tatarazako, Taisen Iguchi, Bmp7 and Lef1 are the down stream effectors of androgen signaling in androgen-induced sex characteristics development in medaka, Endocrinology, 155, 449-462, 2014.02.
4. Yukiko Ogino, Shinichi Miyagawa, Hironori Katoh, Gail S. Prins, Taisen Iguchi, Gen Yamada, Essential functions of androgen signaling emerged through the developmental analysis of vertebrate sex characteristics, Evolution and Development, 13, 315-325, 2011.05.
5. Yukiko Ogino, Hironori Katoh, Shigehiro Kuraku, Gen Yamada, Evolutionary history and functional characterization of androgen receptor genes in jawed vertebrates, Endocrinology, 150, 5415-5427, 2009.12.
1. Yukiko Ogino, Satoshi Ansai, Hirotaka Sakamoto, Eiji Watanabe, Kiyoshi Naruse and Taisen Iguchi, Molecular basis of androgen dependent sex characteristics development and evolution with teleost fishes as model species, 第42回日本分子生物学会年会 , 2019.12, Teleost fishes offer a clue to understanding the evolution of secondary sex characteristics, because they acquired two different subtypes of the AR gene, ARa and ARb, in the teleost-specific whole genome duplication, which might have led to their diversified masculine sex characteristics for appendage development and also sexual behavior. To address the significance of the AR gene duplication, we analyzed the morphological and behavioral phenotypes of medaka mutants for ARa and ARb genes. ARb have predominant function for external masculine phenotypes, and AR might contribute to the development of traits for male-male competition. Both ARs showed the functional redundancy in the courtship behavior, whereas double mutant male of ARa and ARb is infertile due to the loss of courtship behavior, indicating that AR gene duplication can ensure each AR to acquire the attractive function for reproduction, while stabilizing the capability of reproduction by functional redundancy. We also generated the knock-in medaka lines expressing each AR as a fusion protein with FLAG, which allows the production of mClover3 as a separated protein to visualize the spatio-temporal expression and intracellular localization of each AR in vivo. Consistent with the mutant phenotypes, ARb is predominantly expressed in the condensed mesenchyme enriched for expression of androgen effector genes in the anal fin, while ARa is highly expressed in the brain of superior male, indicating the relationship between the expression divergence of each AR and morphological diversification. Our findings would provide a historical explanation for the retention of the duplicated AR copies in the teleost genome..
2. Yukiko Ogino, Hirotaka Sakamoto, Eiji Watanabe and Taisen Iguchi, Diversification of androgen receptor function underlies secondary sex characteristics development of teleost fishes. , 4th Strategic Meeting for Medaka Research,3rd Regional Fish Meeting, 2018.04, Gene duplication is a dominant driving force of evolution. Steroid hormone receptor gene family is thought to have arisen from gene duplication. However, the molecular events which produce new protein functions after the genome duplication have not been fully understood. Teleost fishes present a good model to investigate an accurate evolutionary history of protein function after whole genome duplication, because the teleost-specific whole genome duplication (TSGD) 350 million years ago resulted in a variety of duplicated genes that exist in modern fishes. We focused on the androgen receptor (AR) gene, since two different subtype genes, AR and AR, were generated in the TSGD. AR has retained the ancestral function, whereas the AR evolved as a hyperactive form of AR in the teleost lineage. Results of our combined functional and 3 dimensional analyses of medaka (Oryzias latipes) ARs identified the substitutions that led to changes in protein structure and function between medaka AR and AR. One substitution located within the helices 10/11 of ligand binding domain is sufficient for generating higher transactivation of AR. This amino acid change affects the stability of ligand binding by modifying the hydrogen bonds between ligand and AR.
To further address the functional differences between AR and AR in vivo, we isolated the nonsense mutants of AR and AR from the medaka tilling library. AR have predominant function for external masculine phenotypes, and AR might participate the development of traits for male-male competition. Our findings would provide an historical explanation for the retention of the duplicated AR copies in euteleost genome..
3. 荻野 由紀子, 井口泰泉, Evolutionary diversification of androgen dependent sex characteristics development in teleost fishes., The 22nd International Congress of Zoology, 2016.11.
4. 荻野 由紀子, Functional and evolutionary analyses of androgen receptor gene in teleost fishes: Molecular basis of diversified sex characteristics development, 小型魚類研究会, 2016.08.
Membership in Academic Society
  • Japan Society for Comparative Endocrinology
  • Zoological Society of Japan
  • The Molecular Biology Society of Japan
  • Japanese society of developmental biologists
  • Japanese Society of Scientific Fisheries
  • Japan Society of Endocrine Disrupter Research
Educational Activities
To develop the ability of the creative thinking that is necessary to solve the global environmental problem, I teach a wide variety of biological research areas of biological sciences, such as ecology, environmental science, evolutionary biology, and molecular biology.
In education and research activities for graduate students, I have focused on the hormonal regulatory mechanism of sex characteristics development, which is the basis of biodiversity.
Other Educational Activities
  • 2017.05, I am in charge of the classes regarding the ecology, evolutionary biology iin IUPE general education courses. Student can widely learn the mechanism of biodiversity from the scopes of ecology, evolutionary biology, and environmental science..