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Matsuoka Yuta Last modified date:2021.10.18

Assistant Professor / Division of Molecular Bioinformatics
Department of Chemo-Pharmaceutical Sciences
Faculty of Pharmaceutical Sciences


Graduate School
Undergraduate School


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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/yuta-matsuoka
 Reseacher Profiling Tool Kyushu University Pure
http://bukka.phar.kyushu-u.ac.jp/
Phone
092-642-6625
Fax
092-642-6626
Academic Degree
PhD
Country of degree conferring institution (Overseas)
No
Field of Specialization
Biophysics
Total Priod of education and research career in the foreign country
00years00months
Research
Research Interests
  • A method for the comprehensive analysis of biological aldehydes derived from oxidized lipids
    keyword : Lipid peroxidation, Aldehydes, HPLC, Mass spectrometry
    2018.04.
  • Detection and identification methods for biological free radicals
    keyword : Free radical, Lipid, Protein, Nitroxyl radical, Fluorometric analysis, LCMS
    2015.04~2018.03.
Academic Activities
Papers
1. Matsuoka Y, Yamada KI, Detection and structural analysis of lipid-derived radicals in vitro and in vivo, Free Radic Res, 10.1080/10715762.2021.1881500, 8, 1-9, 2021.02, Lipids can be oxidized by reactive oxygen species, resulting in lipid peroxidation and the formation of reactive metabolites such as lipid-derived electrophiles. These products have been reported to induce inflammation, angiogenesis, and ferroptosis. Lipid peroxidation can produce many different products, each of which performs a different function, and which can be challenging to detect in vivo. The initial products of lipid oxidation are lipid-derived radicals, which can cause extensive chain reactions leading to lipid peroxidation. Hence, the ability to detect lipid radicals may provide information about this important class of molecules and the mechanism by which they cause cellular and tissue damage in a wide range of oxidative conditions. In this review, we report recent scientific advances in the detection of lipid-derived radicals in vitro and in cultured cells. We also introduce the possibility of visualization and structural analysis of lipid-derived radicals generated not only in in cells but also in animal tissue samples from oxidative disease models, using fluorescence-based lipid radicals' detection probes. We anticipate that the various innovative techniques summarized in this paper will be applied and further developed to clarify the role of lipid peroxidation in the pathogenesis of oxidative stress-associated diseases..
2. Matsuoka Y, Izumi Y, Takahashi M, Bamba T, Yamada KI., Method for Structural Determination of Lipid-Derived Radicals, American Chemical Society, 10.1021/acs.analchem.0c00053, 19, 92(10), 6993-7002, 2020.05, Diversified oxidized-lipid molecules are responsible for inflammation and cell death, including ferroptosis. Lipid radicals are the source of these oxidized lipids, which are the initial key molecules in the lipid peroxidation chain reaction. However, owing to their extremely high reactivity and short half-life, an established detection technique is not available. Here, we propose a high-performance liquid chromatography fluorometry and high-resolution tandem mass spectrometry system combined with a fluorescent probe as a structural analysis method for lipid-derived radicals. We detected 132 lipid-derived radicals, including 111 new species, from five polyunsaturated fatty acids. In addition, a database was constructed for which the initial fatty acid could be determined using the radical structure. Further, 12 endogenous lipid-derived radicals were identified in carcinogen-induced liver cancer mouse models. Therefore, this method and its corresponding database will provide novel insights into mechanisms underlying the lipid peroxidation, including the associated inflammation and ferroptosis..
Membership in Academic Society
  • The Pharmaceutical Society of Japan
  • Society of Free Radical Research Japan
  • The Mass Spectrometry Society of Japan
  • The Society of Electron Spin Science and Technology
  • Japanese Society for Chemical Biology