| 1. |
Keigo Shibahara, Koichiro Hayashi, Yasuharu Nakashima, Kunio Ishikawa, Reconstruction of Load-Bearing Segmental Bone Defects Using Carbonate Apatite Honeycomb Blocks, ACS Materials Au, 10.1021/acsmaterialsau.3c00008, 2023.04. |
| 2. |
Koichiro Hayashi, Toshiki Yanagisawa, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Gear-shaped carbonate apatite granules with a hexagonal macropore for rapid bone regeneration, Computational and Structural Biotechnology Journal, 10.1016/j.csbj.2023.03.053, 21, 2514-2523, 2023.04. |
| 3. |
Koichiro Hayashi, Kunio Ishikawa, Endowing Osseointegration Ability to Bioinert Alumina by Carbonate Apatite Coating, Surfaces and Interfaces, 10.1016/j.surfin.2022.102617, 102617, 2023.02. |
| 4. |
Koichiro Hayashi, Toshiki Yanagisawa, Ryo Kishida, Kunio Ishikawa, Effects of Scaffold Shape on Bone Regeneration: Tiny Shape Differences Affect the Entire System, ACS Nano, 10.1021/acsnano.2c03776, 16, 8, 11755-11768, 2022.07, Although studies on scaffolds for tissue generation have mainly focused on the chemical composition and pore structure, the effects of scaffold shape have been overlooked. Scaffold shape determines the scaffold surface area (SA) at the single-scaffold level (i.e., microscopic effects), although it also affects the amount of interscaffold space in the tissue defect at the whole-system level (i.e., macroscopic effects). To clarify these microscopic and macroscopic effects, this study reports the osteogenesis abilities of three types of carbonate apatite granular scaffolds with different shapes, namely, irregularly shaped dense granules (DGs) and two types of honeycomb granules (HCGs) with seven hexagonal channels (∼255 μm in length between opposite sides). The HCGs possessed either 12 protuberances (∼75 μm in length) or no protuberances. Protuberances increased the SA of each granule by 3.24 mm2 while also widening interscaffold spaces and increasing the space percentage in the defect by ∼7.6%. Interscaffold spaces were lower in DGs than HCGs. On DGs, new bone formed only on the surface, whereas on HCGs, bone simultaneously formed on the surface and in intrascaffold channels. Interestingly, HCGs without protuberances formed approximately 30% more new bone than those with protuberances. Thus, even tiny protuberances on the scaffold surface can affect the percentage of interscaffold space, thereby exerting dominant effects on osteogenesis. Our findings demonstrate that bone regeneration can be improved by considering macroscopic shape effects beyond the microscopic effects of the scaffold.. |
| 5. |
Koichiro Hayashi, Toshiki Yanagisawa, Masaya Shimabukuro, Ryo Kishida, Kunio Ishikawa, Granular honeycomb scaffolds composed of carbonate apatite for simultaneous intra- and inter-granular osteogenesis and angiogenesis, Materials Today Bio, 10.1016/j.mtbio.2022.100247, 14, 2022.03, Granular porous calcium phosphate scaffolds are used for bone regeneration in dentistry. However, in conventional granules, the macropore interconnectivity is poor and has varying size. Herein, we developed a productive method for fabricating carbonate apatite honeycomb granules with uniformly sized macropores based on extrusion molding. Each honeycomb granule possesses three hexagonal macropores of ∼290 μm along its diagonal. Owing to these macropores, honeycomb granules simultaneously formed new and mature bone and blood vessels in both the interior and exterior of the granules at 4 weeks after implantation. The honeycomb granules are useful for achieving rapid osteogenesis and angiogenesis.. |
| 6. |
Koichiro Hayashi, Akira Tsuchiya, Masaya Shimabukuro, Kunio Ishikawa, Multiscale Porous Scaffolds Constructed of Carbonate Apatite Honeycomb Granules for Bone Regeneration, Materials & Design, 10.1016/j.matdes.2022.110468, 215, 110468, 2022.03, The use of scaffolds with pores ranging from macroscale to nanoscale (i.e., multiscale pores), is an effective strategy to achieve favorable bone regeneration. Here, we report the fabrication of multiscale porous scaffolds (MPSs) and evaluate the effects of macropores (>100 μm) and micropores ( |
| 7. |
Koichiro Hayashi, Masaya Shimabukuro, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Structurally Optimized Honeycomb Scaffolds with Outstanding Ability for Vertical Bone Augmentation, Journal of Advanced Research, 10.1016/j.jare.2021.12.010, 41, 101-112, 2022.11, IntroductionCases of intractable dental implant require vertical bone augmentation; however, the predicted bone height and volume are difficult to obtain. In vertical bone augmentation, the contact surface between the scaffold and the bone is limited to the bottom face of the scaffold. Furthermore, the strength decrease caused by scaffold resorption leads to collapse of the augmented site, leading to a decrease in the bone volume and height.ObjectivesTo promote bone ingrowth, we fabricated carbonate apatite (i.e., bone mineral) honeycomb (HC) scaffolds with uniaxial channels vertically penetrating the scaffold. Furthermore, we controlled the scaffold resorption rate, eventually the endurability for compression, and the bone height and volume by controlling the strut thickness.MethodsThe channel aperture was controlled to be 230–260 μm to promote bone ingrowth. Furthermore, the strut thicknesses of the HC scaffolds were adjusted to 100, 200, and 300 μm to control the scaffold resorption; these scaffolds were designated as HC100, HC200, and HC300, respectively.ResultsAt 4 weeks post-implantation on rabbit calvarium, all scaffolds had already vertically augmented new bone close to the top surface of the scaffold. In the following 8 weeks, the height and amount of new bone in all scaffolds increased. Notably, HC300 was resorbed synchronously with new bone formation, allowing it to endure the compression from the fasciae for 12 weeks post-implantation. Furthermore, HC300 formed larger-diameter blood vessels than those of HC100 and HC200.ConclusionThe HC scaffolds surpassed the various combined scaffolds and growth factors or stem cells in the ability for vertical bone augmentation. Thus, the HC structure is inherently suitable for vertical bone augmentation. Notably, the HC scaffolds with 300-μm-thick struts enhanced both new bone formation and angiogenesis. This study revealed a structurally suitable design for achieving an outstanding outcome in vertical bone augmentation.. |
| 8. |
Koichiro Hayashi, Masaya Shimabukuro, Kunio Ishikawa, Antibacterial Honeycomb Scaffolds for Achieving Infection Prevention and Bone Regeneration, ACS Applied Materials & Interfaces, 10.1021/acsami.1c20204, 14, 3, 3762-3772, 2022.01, Surgical site infection (SSI) is a severe complication associated with orthopedic bone reconstruction. For both infection prevention and bone regeneration, the framework surface of osteoconductive and bioresorbable scaffolds must be locally
modified by minimum antibacterial substances, without sacrificing the osteoconductivity of the scaffold framework. In this study, we fabricated antibacterial honeycomb scaffolds by replacing carbonate apatite, which is the main component of the scaffold, with silver phosphate locally on the scaffold surface via dissolution−precipitation reactions. When the silver content was 9.9 × 10−4 wt %, the honeycomb scaffolds showed antibacterial activity without cytotoxicity and allowed cell proliferation, differentiation, and mineralization. Furthermore, the antibacterial honeycomb scaffolds perfectly prevented bacterial infection in vivo in the presence of methicillin-resistant Staphylococcus aureus, formed new bone at 2 weeks after surgery, and were gradually replaced with a new bone. Thus, the antibacterial honeycomb scaffolds achieved both infection prevention and bone regeneration. In contrast, severe infection symptoms, including abscess formation, osteolytic lesions, and inflammation, occurred 2 weeks after surgery when honeycomb scaffolds without silver phosphate modification were implanted. Nevertheless, the unmodified honeycomb scaffolds eliminated bacteria and necrotic bone through their scaffold channels, resulting in symptom improvement and bone formation. These results suggest that the honeycomb structure is inherently effective in hindering bacterial growth. This novel insight may contribute to the development of antibacterial scaffolds. Moreover, our modification method is useful for providing antibacterial activity to various biomaterials.. |
| 9. |
Koichiro Hayashi,Ryo Kishida,Akira Tsuchiya,Kunio Ishikawa, Channel Aperture Characteristics of Carbonate Apatite Honeycomb Scaffolds Affect Ingrowths of Bone and Fibrous Tissues in Vertical Bone Augmentation, Bioengineering, 9, 11, 627, 2022.11. |
| 10. |
Koichiro Hayashi, Kunio Ishikawa, Honeycomb scaffolds capable of ectopic osteogenesis: Histological evaluation of osteoinduction mechanism, Nano Select, 10.1002/nano.202000283, 3, 1, 60-77, 2022.01. |
| 11. |
Keigo Shibahara, Koichiro Hayashi, Yasuharu Nakashima, Kunio Ishikawa, Effects of Channels and Micropores in Honeycomb Scaffolds on the Reconstruction of Segmental Bone Defects, Frontiers in Bioengineering and Biotechnology, 10.3389/fbioe.2022.825831, 10, 2022.03. |
| 12. |
Keiko Kudoh, Naoyuki Fukuda, Kazuya Akita, Takaharu Kudoh, Natsumi Takamaru, Naito Kurio, Koichiro Hayashi, Kunio Ishikawa, Youji Miyamoto, Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure, Journal of Materials Science: Materials in Medicine , 10.1007/s10856-022-06710-2, 34, 1, 2022.12. |
| 13. |
Masaya Shimabukuro, Koichiro Hayashi, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Surface functionalization with copper endows carbonate apatite honeycomb scaffold with antibacterial, proangiogenic, and pro-osteogenic activities, Biomaterials Advances, 10.1016/j.bioadv.2022.212751, 212751, 2022.03. |
| 14. |
Pery Freitas, Ryo Kishida, Koichiro Hayashi, Akira Tsuchiya, Masaya Shimabukuro, Kunio Ishikawa, Fabrication and histological evaluation of porous carbonate apatite blocks using disodium hydrogen phosphate crystals as a porogen and phosphatization accelerator, Journal of Biomedical Materials Research Part A, 10.1002/jbm.a.37374, 2022.02. |
| 15. |
Maab Elsheikh, Ryo Kishida, Koichiro Hayashi, Akira Tsuchiya, Masaya Shimabukuro, Kunio Ishikawa, Effects of Pore Interconnectivity on Bone Regeneration in Carbonate Apatite Blocks, Regenerative Biomaterials, 10.1093/rb/rbac010, rbac010, 2022.02. |
| 16. |
Tansza Setiana Putri, Sunarso, Koichiro Hayashi, Kanji Tsuru, Kunio Ishikawa, Feasibility study on surface morphology regulation of β-tricalcium phosphate bone graft for enhancing cellular response, Ceramics International, 10.1016/j.ceramint.2022.02.200, 48, 9, 13395-13399, 2022.05. |
| 17. |
Masaya Shimabukuro, Koichiro Hayashi, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, No-Observed-Effect Level of Silver Phosphate in Carbonate Apatite Artificial Bone on Initial Bone Regeneration, ACS Infectious Diseases, https://doi.org/10.1021/acsinfecdis.1c00480, 8, 1, 159-169, 2022.01. |
| 18. |
Kunio Ishikawa, Pery Freitas, Ryo Kishida, Koichiro Hayashi, Akira Tsuchiya, Fabrication of vaterite blocks from a calcium hydroxide compact, Ceramics International, 10.1016/j.ceramint.2021.10.206, 48, 3, 4153-4157 , 2022.02. |
| 19. |
Masaya Shimabukuro,Koichiro Hayashi,Ryo Kishida,Akira Tsuchiya,Kunio Ishikawa, Effects of carbonate ions in phosphate solution on the fabrication of carbonate apatite through a dissolution–precipitation reaction, Ceramics International, 10.1016/j.ceramint.2021.09.188, 48, 1, 1032-1037, 2022.01. |
| 20. |
Koichiro Hayashi, Masaya Shimabukuro, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Honeycomb Scaffolds Capable of Achieving Barrier Membrane-Free Guided Bone Regeneration, Materials Advances, 10.1039/d1ma00698c, 2, 23, 7638-7649 , 2021.12, Barrier membrane-free guided bone regeneration (GBR) with a synthetic scaffold may resolve the current challenges in vertical bone augmentation. To realize such GBR, we fabricated carbonate apatite honeycomb (HC) scaffolds capable of preventing soft tissue invasion and enhancing bone ingrowth. These HC scaffolds with 230-, 460-, and 630 μm-aperture channels were designated as HC230, HC460, and HC630, respectively. They were constructed by interconnecting carbonate apatite microspheres; they possessed micropores and nanopores in the struts and were implanted on the rabbit calvarium. The amount of new bone and soft tissues in the HC scaffolds significantly increased and decreased, respectively, with the decrease in the channel aperture size. The new bone height in HC230 at 4 and 12 weeks post-implantation was 3.4 ± 0.5 and 3.8 ± 0.2 mm, respectively, reaching the top edge of the struts. The percent volume of new bone in HC230 at 4 and 12 weeks post-implantation was 38.6% ± 2.2% and 49.9% ± 1.5%, respectively. These findings demonstrated that HC230 augmented faster, higher, and a greater amount of vertical bone growth than the reported combinations of scaffolds and growth factors or barrier membranes. Therefore, the multiscale-architectural control of HC scaffolds may pioneer barrier membrane-free GBR.. |
| 21. |
Koichiro Hayashi,Nao Kato,Masaki Kato,Kunio Ishikawa, Impacts of Channel Direction on Bone Tissue Engineering in 3D-Printed Carbonate Apatite Scaffolds, Materials & Design, 10.1016/j.matdes.2021.109686, 204, 109686, 2021.06, Although the channel architecture of a scaffold is critical for bone regeneration, little is known for the channel direction. In this study, four types of carbonate apatite cylindrical scaffolds; scaffolds with biaxial channels (VH-scaffold), with uniaxial vertical channels (V-scaffold), with uniaxial horizontal channels (H-scaffold), and without channels (N-scaffold), were implanted in a rabbit femur defect for 4 and 12 weeks. Although the largest bone was formed 4 weeks post-implantation in the VH-scaffold, newly formed bone disappeared with the scaffold after 12 weeks. Thus, biaxial channels resulted in the rapid dissolution of the scaffold and were counterproductive in long-term bone regeneration. The V-scaffold that had channels connected to the periosteum was gradually resorbed throughout 12 weeks post-implantation. The percentage of mineralized bone in the V-scaffolds was equal to that in the natural bone. The resorption and bone percentage of H-scaffolds that had no channels connected to the periosteum were slower and lower, respectively, than those of V-scaffolds. Thus, channels should be connected to the periosteum to achieve smooth replacement by the new bone. In the N-scaffold, much less bone was formed inside the scaffold. This study contributes to providing a design guide for scaffold development in bone engineering.. |
| 22. |
Koichiro Hayashi, Kunio Ishikawa, Honeycomb Scaffolds Fabricated Using Extrusion Molding and Sphere Packing Theory for Bone Regeneration, ACS Applied Bio Materials, 10.1021/acsabm.0c01279, 4, 1, 721-730, 2021.01. |
| 23. |
Keigo Shibahara,Koichiro Hayashi,Yasuharu Nakashima,Kunio Ishikawa, Honeycomb Scaffold-Guided Bone Reconstruction of Critical-Sized Defects in Rabbit Ulnar Shafts, ACS Applied Bio Materials, 10.1021/acsabm.1c00533, 2021.09. |
| 24. |
Yuta Sakemi, Koichiro Hayashi, Akira Tsuchiya, Yasuharu Nakashima, Kunio Ishikawa, Reconstruction of critical-size segmental defects in rat femurs using carbonate apatite honeycomb scaffolds, Journal of Biomedical Materials Research Part A, 10.1002/jbm.a.37157, 109, 9, 1613-1622, 2021.09. |
| 25. |
Cheng Zhang, Koichiro Hayashi, Kunio Ishikawa, Enhancement of bone to polylactic acid plate bonding by carbonate apatite coating, Ceramics International, 10.1016/j.ceramint.2021.06.252, 47, 20, 28348-28356, 2021.10. |
| 26. |
Ryo Kishida,Maab Elsheikh,Koichiro Hayashi,Akira Tsuchiya,Kunio Ishikawa, Fabrication of highly interconnected porous carbonate apatite blocks based on the setting reaction of calcium sulfate hemihydrate granules, Ceramics International, https://doi.org/10.1016/j.ceramint.2021.03.324, 47, 14, 19856-19863, 2021.07. |
| 27. |
Koichiro Hayashi, Kunio Ishikawa, Effects of Nanopores on the Mechanical Strength, Osteoclastogenesis, and Osteogenesis in Honeycomb Scaffolds（selected as a Journal of Materials Chemistry B HOT Paper）, Journal of Materials Chemistry B, 10.1039/D0TB01498B, 2020.08, The scaffold chemical composition and pore architecture are critical for successful bone regeneration. Although the effects of chemical composition, micron-scale pores, and macropores (Z100 mm) are known, those of nanometer-scale pores (nanopores) are unknown. Here, honeycomb scaffolds (HCSs) composed of carbonate apatite and bone mineral, were fabricated with three distinct nanopore volumes, while other parameters were comparable between HCSs. Their compressive strengths and nanopore volumes linearly correlated. The HCSs were implanted into critical-sized bone defects (CSDs) in the rabbit femur distal epiphyses. The nanopore volume affected both osteoclastogenesis and osteogenesis. HCSs with nanopore volumes of Z0.15 cm3 g-1 promoted osteoclastogenesis, contributing to bone maturation and bone formation within 4 weeks. However, HCSs with nanopore volumes of 0.07 cm3 g-1 promoted significantly less bone maturation and neoformation. Nevertheless, HCSs with nanopore volumes of Z0.18 cm3 g-1 did not undergo continuous bone regeneration throughout the 12 week period due to excessive osteoclastogenesis, which favored HCS resorption over bone neoformation. When the nanopore volume was 0.15 cm3 g-1, osteoclastogenesis and osteogenesis progressed harmonically, resulting in HCS replacement with new bone. Our results demonstrate that the nanopore volume is critical for controlling osteoclastogenesis and osteogenesis. These insights may help establish a coherent strategy for developing scaffolds for different applications.. |
| 28. |
Michihiro Nakamura, Koichiro Hayashi, Junna Nakamura, Chihiro Mochizuki, Takuya Murakami, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Near-Infrared Fluorescent Thiol-Organosilica Nanoparticles That Are Functionalized with IR-820 and Their Applications for Long-Term Imaging of in Situ Labeled Cells and Depth-Dependent Tumor in Vivo Imaging , Chemistry of Materials, 10.1021/acs.chemmater.0c01414, 2020.08, Thiol-organosilica nanoparticles that are internally functionalized with IR-820 (thiol-OS/IR820) were prepared via a one-pot process for near-infrared (NIR) fluorescence in vivo imaging. Thiol-OS/IR820 demonstrated broad-band emissive NIR fluorescence with multiple new fluorescent peaks that differed from those of the IR-820 molecule and upconversion fluorescence originated from thiol-OS. Thiol-OS/IR820 was biocompatible and did not show significant toxicity in vitro and in vivo. We conducted in vivo tracking of in situ labeled cells against subcutaneous xenograft cells. The in vivo imaging showed a migration and an accumulation of the in situ labeled cells to the site of xenograft cells. Then, a reduction of the grafted cells was observed after 3 weeks. Next, we conducted in vivo tumor imaging of a mouse with a subcutaneous xenograft tumor using intravenous administration of thiol-OS/IR820. Using three wavelengths of light emission, depth-dependent NIR fluorescence imaging of a mouse with a subcutaneous xenograft tumor was conducted. The accumulation of particles in the tumor tissue due to the enhanced permeability and retention (EPR) effect was observed depth-dependently. NIR fluorescence in vivo imaging using thiol-OS/IR820 is useful for long-term observation and shows substantial promise for the visualization of novel biological phenomena in vivo.. |
| 29. |
Hyungjin Kim, Daniel Röth, Yasuhiro Isoe, Koichiro Hayashi, Chihiro Mochizuki, Kalkum Markus, Michihiro Nakamuraa, Protein Corona Components of Polyethylene Glycol-conjugated Organosilica Nanoparticles Modulates Macrophage Uptake, Colloids and Surfaces B: Biointerfaces
, 10.1016/j.colsurfb.2020.111527, 2020.12. |
| 30. |
Koichiro Hayashi, Atsuto Tokuda, Jin Nakamura, Ayae Sugawara-Narutaki, Chikara Ohtsuki, Tearable and fillable composite sponges capable of heat generation and drug release in response to alternating magnetic field, Materials, 10.1039/10.3390/ma13163637, 2020.08. |
| 31. |
Rui Shi,Koichiro Hayashi,Kunio Ishikawa, Rapid Osseointegration Bestowed by Carbonate Apatite Coating of Rough Titanium, Advanced Materials Interfaces, 10.1002/admi.202000636, 2020.07, Titanium (Ti) implants that realize rapid osseointegration are required for favorable outcomes. Rough implant surfaces favor osseointegration, hence, coating implants with natural bone mineral, i.e., carbonate apatite (CO3Ap), may be effective for osseointegration. To achieve rapid osseointegration, rough-Ti substrates are coated with CO3Ap (CO3Ap-Ti) and the effects are evaluated in vitro and in vivo. For comparison, rough-Ti without coating (rough-Ti) and calcite-coated rough-Ti (calcite-Ti) substrates are fabricated. The adhesive strengths of calcite and CO3Ap to the substrates are ≈56.6 and ≈76.8 MPa, respectively, being significantly higher than the strength defined in ISO13779-2 (15 MPa). Calcite and CO3Ap coatings significantly promote preosteoblastic MC3T3-E1 cell proliferation. Additionally, the CO3Ap coating promotes higher osteogenic differentiation activity than the calcite coating. Implantation of CO3Ap-Ti into rabbit tibia defects prompts bone maturation, compared to calcite-Ti or rough-Ti implantation. The bone-implant contact percentage with CO3Ap-Ti and calcite-Ti is higher than that with rough-Ti. Consequently, CO3Ap-Ti acquires a robust bond with the host bone at an early stage (4 weeks postimplantation), compared to calcite-Ti or rough-Ti: the CO3Ap-Ti–bone bonding strength is ≈1.9- and ≈5.5-fold higher than that of calcite-Ti and rough-Ti, respectively. Thus, CO3Ap coating of Ti implants effectively achieve rapid osseointegration.. |
| 32. |
Koichiro Hayashi, Melvin L. Munar, Kunio Ishikawa, Effects of macropore size in carbonate apatite honeycomb scaffolds on bone regeneration, Materials Science and Engineering: C, https://doi.org/10.1016/j.msec.2020.110848, 111, 110848, 2020.06, The pore architecture of scaffolds is a critical factor for angiogenesis and bone regeneration. Although the effects of scaffold macropore size have been investigated, most scaffolds feature macropores with poor uniformity and interconnectivity, and other parameters (e.g., microporosity, chemical composition, and strut thickness) differ among scaffolds. To clarify the threshold of effective macropore size, we fabricated honeycomb scaffolds (HCSs) with distinct macropore (i.e., channel) sizes (~100, ~200, and ~300 μm). The HCSs were composed of AB-type carbonate apatite with ~8.5% carbonate ions, i.e., the same composition as human bone mineral. Their honeycomb architecture displayed uniformly sized and orderly arranged channels with extremely high interconnectivity, and all the HCSs displayed ~100-μm-thick struts and 0.06 cm3 g−1 of micropore volume. The compressive strengths of
HCSs with ~100-, ~200-, and ~300-μm channels were higher than those of reported scaffolds, and decreased with increasing channel size: 62 ± 6, 55 ± 9, and 43 ± 8 MPa, respectively. At four weeks after implantation in rabbit femur bone defects, new bone and blood vessels were formed in all the channels of these HCSs. Notably, the ~300-μm channels were extensively occupied by new bone. We demonstrated that high interconnectivity and uniformity of channels can decrease the threshold of effective macropore size, enabling the scaffolds to maintain high mechanical properties and osteogenic ability and serve as implants for weight-bearing areas.. |
| 33. |
Tansza S. Putri, Koichiro Hayashi, Kunio Ishikawa, Fabrication of three-dimensional interconnected porous blocks composed of robust carbonate apatite frameworks, Ceramics International, https://doi.org/10.1016/j.ceramint.2020.05.076, 2020.05. |
| 34. |
Koichiro Hayashi, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Granular Honeycombs Composed of Carbonate Apatite, Hydroxyapatite, and β-Tricalcium Phosphate as Bone Graft Substitutes: Effects of Composition on Bone Formation and Maturation, ACS Applied Bio Materials, https://doi.org/10.1021/acsabm.0c00060, 3, 3, 1787-1795, 2020.03. |
| 35. |
Thet Thet Swe, Khairul Anuar Shariff, Hasmaliza Mohamad, Kunio Ishikawa, Koichiro Hayashi, Mohamad Hafizi Abu Bakar, Behavioural response of cells and bacteria on single and multiple doped Sr and Ag S53P4 Sol-Gel Bioglass, Ceramics International, https://doi.org/10.1016/j.ceramint.2020.04.094, 2020.04. |
| 36. |
Tansza Setiana Putri, Koichiro Hayashi, Kunio Ishikawa, Bone regeneration using β-tricalcium phosphate (β-TCP) block with interconnected pores made by setting reaction of β-TCP granules, Journal of Biomedical Materials Research Part A, 108, 625-632, 2020.01. |
| 37. |
Rui Shi, Koichiro Hayashi, LT Bang, Kunio Ishikawa, Effects of surface roughening and calcite coating of titanium on cell growth and differentiation, Journal of Biomaterials Applications, 34, 917-927, 2020.02. |
| 38. |
Koichiro Hayashi, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Carbonate Apatite Micro-Honeycombed Blocks Generate Bone Marrow-Like Tissues as well as Bone, Advanced Biosystems, 2019.09. |
| 39. |
Koichiro Hayashi, Ryo Kishida, Akira Tsuchiya, Kunio Ishikawa, Honeycomb blocks composed of carbonate apatite, β-tricalcium phosphate, and hydroxyapatite for bone regeneration: effects of composition on biological responses, Materials Today Bio, 4, 100031, 2019.09, Synthetic scaffolds exhibiting bone repair ability equal to that of autogenous bone are required in the fields of orthopedics and dentistry. A suitable synthetic bone graft substitute should induce osteogenic differentiation of mesenchymal stem cells, osteogenesis, and angiogenesis. In this study, three types of honeycomb blocks (HCBs), composed of hydroxyapatite (HAp), β-tricalcium phosphate (TCP), and carbonate apatite (CO3Ap), were fabricated, and the effects of HCB composition on bone formation and maturation were investigated. The HC structure was selected to promote cell penetration and tissue ingrowth. HAp and β-TCP HCBs were fabricated by extrusion molding followed by sintering. The CO3Ap HCBs were fabricated by extrusion molding followed by sintering and dissolution-precipitation reactions. These HCBs had similar macroporous structures: all harbored uniformly distributed macropores (∼160 μm) that were regularly arrayed and penetrated the blocks unidirectionally. Moreover, the volumes of macropores were nearly equal (∼0.15 cm3/g). The compressive strengths of CO3Ap, HAp, and β-TCP HCBs were 22.8 ± 3.5, 34.2 ± 3.3, and 24.4 ± 2.4 MPa, respectively. Owing to the honeycomb-type macroporous structure, the compressive strengths of these HCBs were higher than those of commercial scaffolds with intricate three-dimensional or unidirectional macroporous structure. Notably, bone maturation was markedly faster in CO3Ap HCB grafting than in β-TCP and HAp HCB grafting, and the mature bone area percentages for CO3Ap HCBs at postsurgery weeks 4 and 12 were 14.3- and 4.3-fold higher and 7.5- and 1.4-fold higher than those for HAp and β-TCP HCBs, respectively. The differences in bone maturation and formation were probably caused by the disparity in concentrations of calcium ions surrounding the HCBs, which were dictated by the inherent material resorption behavior and mechanism; generally, CO3Ap is resorbed only by osteoclastic resorption, HAp is not resorbed, and β-TCP is rapidly dissolved even in the absence of osteoclasts. Besides the composition, the microporous structure of HC struts, inevitably generated during the formation of HCBs of various compositions, may contribute to the differences in bone maturation and formation.. |
| 40. |
Koichiro Hayashi, Melvin L. Munar, Kunio Ishikawa, Carbonate apatite granules with uniformly sized pores that arrange regularly and penetrate straight through granules in one direction for bone regeneration, Ceramics International, 45, 15429-15434, 2019.05. |
| 41. |
Yuta Sakemi, Koichiro Hayashi, Akira Tsuchiya, Yasuharu Nakashima, Kunio Ishikawa, Fabrication and Histological Evaluation of Porous Carbonate Apatite Block from Gypsum Block Containing Spherical Phenol Resin as a Porogen, Materials, https://doi.org/10.3390/ma12233997, 12, 3997, 2019.12. |
| 42. |
Kunio Ishikawa, Tya Arifta, Koichiro Hayashi, Kanji Tsuru, Fabrication and Evaluation of Interconnected Porous Carbonate Apatite from Alpha Tricalcium Phosphate Spheres, Journal of Biomedical Materials Research: Part B - Applied Biomaterials, 107, 2, 269-277, 2019.02. |
| 43. |
Koichiro Hayashi, Takuma Maruhashi, Wataru Sakamoto, Toshinobu Yogo, Organic-inorganic Hybrid Hollow Nanoparticles Suppress Oxidative Stress and Repair Damaged Tissues for Treatment of Hepatic Fibrosis, Advanced Functional Materials, 28, 1706332, 2018.03, Current therapeutic options for the treatment of liver fibrosis are limited, and transplantation is often the only effective option for end-stage fibrotic diseases. To overcome this problem, a nanoparticle-based treatment as an alternative to transplantation is developed. Multifunctional organic–inorganic hybrid hollow nanoparticles (HNPs) containing silibinin are synthesized by mixing precursors in ammonia water at 60 °C for 1 min. The HNPs are mainly composed of siloxanes and disulfides and have surface thiols. The disulfides are cleaved by intracellular glutathione and reduced to thiols, leading to the deformation of the HNPs. Silibinin molecules are released through the cracks formed by HNP deformation. Furthermore, the HNPs suppress the generation of hydroxyl radicals, a major cause of liver fibrosis, via sulfenylation reactions of HNP thiols. Retinol-modified HNPs target Kupffer cells and hepatic stellate cells, which are essential for hepatic fibrogenesis. The combined suppression of hydroxyl radical generation and release of silibinin using the HNPs decreases the proportion of fibrotic tissues and improves hepatic function. The therapeutic efficacy is greater than can be achieved by the suppression of hydroxyl radical generation alone and the injection of silibinin alone. Thus, HNPs are promising for the treatment of liver fibrosis.. |
| 44. |
Koichiro Hayashi, Shota Yamada, Hikaru Hayashi, Wataru Sakamoto, Toshinobu Yogo, Red Blood Cell-like Particles with the Ability to Avoid Lung and Spleen Accumulation for the Treatment of Liver Fibrosis, Biomaterials, 156, 45-55, 2018.02. |
| 45. |
Koichiro Hayashi, Shota Yamada, Wataru Sakamoto, Eri Usugi, Masatoshi Watanabe, and Toshinobu Yogo, Red Blood Cell-Shaped Microparticles with Red Blood Cell Membrane Demonstrate Prolonged Circulation Time in Blood, ACS Biomaterials Science & Engineering, 2018.07. |
| 46. |
Koichiro Hayashi, Hikaru Hayashi, Shota Yamada, Wataru Sakamoto, Toshinobu Yogo, Cellulose-Based Molecularly Imprinted Red-Blood-Cell-Like Microparticles for the Selective Capture of Cortisol, Carbohydrate Polymers, 193, 173-178, 2018.04, Magnetite-nanoparticle-containing red-blood-cell-like-microparticles (M-RBC-MPs) with a selective ability for trapping cortisol (COR) were synthesized by an electrospray technique of a molecularly imprinted ethyl(hydroxyethyl) cellulose (EHEC)-based precursor. The as-synthezied M-RBC-MPs were ∼3-μm-disks with a dent. MRBC-MPs contained magnetite nanoparticles below 15 nm in diameter, which exhibited magnetization and no room-temperature coercivity. The molecularly imprinted M-RBC-MPs (MI-M-RBC-MPs) passed through pores less than their diameter. The MI-M-RBC-MPs selectively trapped COR from a solution containing molecules similar to COR, whereas non-imprinted M-RBC-MPs did not trap COR. Furthermore, magnets were used to capture the water-dispersed MI-M-RBC-MPs flowing in a tube. Based on the above results, MI-M-RBC-MPs may selectively trap COR while simultaneously circulating in the blood, followed by their removal from the blood using magnets.. |
| 47. |
Koichiro Hayashi, Atsuto Tokuda, and Wataru Sakamoto, Hydroxyl Radical-Suppressing Mechanism and Efficiency of Melanin-Mimetic Nanoparticles (invited), International Journal of Molecular Sciences, 2018.08. |
| 48. |
Naoya Ozawa, Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, Synthesis of titania nanoparticle-dispersed hybrid membranes fromallyloxytitanium and phosphonic acid derivatives for fuel cell, Journal of Membrane Science, 563, 221-228, 2018.10. |
| 49. |
Kunio Ishikawa, Youji Miyamoto, Akira Tsuchiya, Koichiro Hayashi, Kanji Tsuru and Go Ohe, Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes, Materials, 11, 1993, 2018.10. |
| 50. |
Hirokazu Miki, Shingen Nakamura, Asuka Oda, Hirofumi Tenshin, Jumpei Teramachi, Masahiro Hiasa, Ariunzaya Bat-Erdene, Yusaku Maeda, Masahiro Oura, Mamiko Takahashi, Masami Iwasa, Takeshi Harada, Shiro Fujii, Kiyoe Kurahashi, Sumiko Yoshida, Kumiko Kagawa, Itsuro Endo, Kenichi Aihara, Mariko Ikuo, Kohji Itoh, Koichiro Hayashi, Michihiro Nakamura and Masahiro Abe, Effective impairment of myeloma cells and their progenitors by hyperthermia, Oncotarget, 9, 10307-10316, 2018.02. |
| 51. |
Kouta Noritake, Wataru Sakamoto, Isamu Yuitoo, Teruaki Takeuchi, Koichiro Hayashi, Toshinobu Yogo, Fabrication of Lead-Free Piezoelectric Li2CO3-Added (Ba,Ca)(Ti,Sn)O3 Ceramics under Controlled Low Oxygen Partial Pressure and Their Properties, Japanese Journal of Applied Physics, 57, 021501-1‒021501-7, 2018.02. |
| 52. |
Masaya Takemoto, Masatomo Hattori, Koichiro Hayashi, Shin-ichi Yamaura, Wei Zhang, Wataru Sakamoto, Toshinobu Yogo, Metallic Glass Separators for Fuel Cells at Intermediate Temperatures, Materials Letters, 206, 87–90, 2017.11. |
| 53. |
Wataru Sakamoto, Kouta Noritake, Hiroki Ichikawa, Koichiro Hayashi, Toshinobu Yogo, Fabrication and Properties of Nonreducible Lead-Free Piezoelectric Mn-Doped (Ba,Ca)TiO3 Ceramics, Ceramics International, 43, S166‒S171, 2017.08. |
| 54. |
Masaya Takemoto, Koichiro Hayashi, Shin-ichi Yamaura, Wei Zhang, Wataru Sakamoto, Toshinobu Yogo, Synthesis of Inorganic-organic Hybrid Membranes Consisting of Organotrisiloxane Linkages and Their Fuel Cell Properties at Intermediate Temperatures, Polymer, 120, 264–271, 2017.06. |
| 55. |
Michihiro Nakamura, Koichiro Hayashi, Hitoshi Kubo, Masafumi Harada, Keisuke Izumi, Yoshihiro Tsuruo, and Toshinobu Yogo, Mesoscopic Multimodal Imaging Provides New Insight to Tumor Tissue Evaluation: An Example of Macrophage Imaging of Hepatic Tumor using Organosilica Nanoparticles, Scientific Reports, 7, 3953, 2017.06. |
| 56. |
Koichiro Hayashi, Yusuke Sato, Hiroki Maruoka, Wataru Sakamoto, and Toshinobu Yogo, Organic-Inorganic Hybrid Nanoparticles for Tracking the Same Cells Seamlessly at the Cellular, Tissue, and Whole Body Levels, ACS Biomaterials Science & Engineering, 3, 1129–1135, 2017.06. |
| 57. |
Michihiro Nakamura, Koichiro Hayashi, Hitoshi Kubo, Takafumi Kanadani, Masafumi Harada, Toshinobu Yogo, Relaxometric Property of Organosilica Nanoparticles Internally Functionalized with Iron Oxide and Fluorescent Dye for Multimodal Imaging, Journal of Colloid and Interface Science, 49, 127–135, 2017.04. |
| 58. |
Koichiro Hayashi, Yoshitaka Sato, Wataru Sakamoto, Toshinobu Yogo, Theranostic Nanoparticles for MRI-Guided Thermochemotherapy: Tight Clustering of Magnetic Nanoparticles Boosts Relaxivity and Heat-Generation Power, ACS Biomaterials Science & Engineering, 3, 95–105, 2017.01. |
| 59. |
Koichiro Hayashi, Takuma Maruhashi, Michihiro Nakamura, Wataru Sakamoto, Toshinobu Yogo, One-Pot Synthesis of Dual Stimulus-Responsive Degradable Hollow Hybrid Nanoparticles for Image-guided Trimodal Therapy, Advanced Functional Materials, 26, 8613–8622, 2016.12. |
| 60. |
Naoya Ozawa, Koichiro Hayashi, Shin-ichi Yamaura, Wei Zhang, Wataru Sakamoto, Toshinobu Yogo, Synthesis of Inorganic-Organic Hybrid Membranes Consisting of Triazole Linkages Formed by the Azide-Alkyne Click Reaction, Journal of Membrane Science, 517, 21–29, 2016.11. |
| 61. |
Rika Maruyama, Wataru Sakamoto, Isamu Yuitoo, Teruaki Takeuchi, Koichiro Hayashi, Toshinobu Yogo, Photocurrent Enhancement of Chemically Synthesized Ag Nanoparticle-Embedded BiFeO3 Thin Films, Japanese Journal of Applied Physics, 55, 10TA14-1, 2016.10. |
| 62. |
Koji Takahashi, Junji Umeda, Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, One-Pot Synthesis of Inorganic/Organic Hybrid Membranes from Organoalkoxysilane, Hydroimidazole Derivative, and Cyclic Sulfonic Acid Ester, Journal of Materials Science, 51, 3398–3407, 2016.04. |
| 63. |
Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, Smart Ferrofluid with Quick Gel Transformation in Tumors for MRI-Guided Local Magnetic Thermochemotherapy, Advanced Functional Materials, 26, 1708–1718, 2016.03. |
| 64. |
Tatsuo Hoshino, Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, One-Pot Synthesis of Proton-Conductive Inorganic–Organic Hybrid Membranes from Organoalkoxysilane and Phosphonic Acid Derivatives, Journal of Membrane Science, 502, 133–140, 2016.03. |
| 65. |
Sergey Zhukov, Yuri A. Genenko, Jurij Koruza, Jan Schultheiß, Heinz von Seggern, Wataru Sakamoto, Hiroki Ichikawa, Tatsuro Murata, Koichiro Hayashi, Toshinobu Yogo, Effect of Texturing on Polarization Switching Dynamics in Ferroelectric Ceramics, Applied Physics Letters, 108, 012907, 2016.01. |
| 66. |
Takeshi Katayama, Wataru Sakamoto, Isamu Yuitoo, Teruaki Takeuchi, Koichiro Hayashi, Toshinobu Yogo, Enhancement of Photoinduced Electrical Properties of Al-Doped ZnO/BiFeO3 Layered Thin Films Prepared by Chemical Solution Deposition, Japanese Journal of Applied Physics, 54, 10NA05, 2015.09. |
| 67. |
Michihiro Nakamura, Koichiro Hayashi, Mutsuki Nakano, Takafumi Kanadani, Kazue Miyamoto, Toshinari Kori, Kazuki Horikawa, Identification of Polyethylene Glycol-Resistant Macrophages on Stealth Imaging in Vitro Using Fluorescent Organosilica Nanoparticles, ACS Nano, 9, 1058–1071, 2015.02. |
| 68. |
Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, Iodinated Silica/Porphyrin Hybrid Nanoparticles for X-Ray Computedtomography/Fluorescence Dual-Modal Imaging of Tumors, Journal of Asian Ceramic Societies, 2, 429–434, 2014.12. |
| 69. |
Kiyofumi Katagiri, Keiko Ohta, Kaori Sako, Kei Inumaru, Koichiro Hayashi, Yoshihiro Sasaki, Kazunari Akiyoshi, Development and Potential Theranostic Applications of a Self-Assembled Hybrid of Magnetic Nanoparticle Clusters with Polysaccharide Nanogels, ChemPlusChem, 79, 1631–1637, 2014.11. |
| 70. |
Koichiro Hayashi, Michihiro Nakamura, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Wataru Sakamoto, Toshinobu Yogo, Kazunori Ishimura, Magnetically Responsive Smart Nanoparticles for Cancer Treatment with a Combination of Magnetic Hyperthermia and Remote-Control Drug Release, Theranostics, 4, 834–844, 2014.06. |
| 71. |
Koichiro Hayashi, Michihiro Nakamura, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Kazunori Ishimura, Photostable Iodinated Silica/Porphyrin Hybrid Nanoparticles with Heavy-Atom Effect for Wide-Field Photodynamic/Photothermal Therapy Using Single Light Source, Advanced Functional Materials, 24, 503–513, 2014.01. |
| 72. |
Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, One-Pot Synthesis of Magnetic Nanoparticles Assembled on Polysiloxane Rod and Their Response to Magnetic Field, Colloid and Polymer Science, 291, 2837–2842, 2013.12. |
| 73. |
Koichiro Hayashi, Michihiro Nakamura, Wataru Sakamoto, Toshinobu Yogo, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Kazunori Ishimura, Superparamagnetic Nanoparticle Clusters for Cancer Theranostics Combining Magnetic Resonance Imaging and Hyperthermia Treatment, Theranostics, 3, 366–376, 2013.04. |
| 74. |
Koichiro Hayashi, Michihiro Nakamura, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Kazunori Ishimura, Gold Nanoparticle Cluster/Plasmon-Enhanced Fluorescent Silica Core-Shell Nanoparticles for X-Ray Computed Tomography/Fluorescence Dual-Mode Imaging of Tumors, Chemical Communications, 49, 5334–5336, 2013.04. |
| 75. |
Koichiro Hayashi, Michihiro Nakamura, Kazunori Ishimura, Near-Infrared Fluorescent Silica-Coated Gold Nanoparticle Clusters for X-Ray Computed Tomography/Optical Dual Modal Imaging of the Lymphatic System, Advanced Healthcare Materials, 2, 756–763, 2013.03. |
| 76. |
Michihiro Nakamura, Kazunori Miyamoto, Koichiro Hayashi, Aziz Awaad, Masahito Ochiai, Kazunori Ishimura, Time-Lapse Fluorescence Imaging and Quantitative Single Cell and Endosomal Analysis of Peritoneal Macrophages Using Fluorescent Organosilica Nanoparticles, Nanomedicine: Nanotechnology, Biology, and Medicine, 9, 274–283, 2013.02. |
| 77. |
Koichiro Hayashi, Michihiro Nakamura, Wataru Sakamoto, Toshinobu Yogo, Kazunori Ishimura, Synthesis and 3D Hierarchical Organization of 2D Structured Iron Oxide Based on Enzymatic Structure, Activity and Thermostability, Materials Research Bulletin, 47, 3959–3964, 2012.12. |
| 78. |
Michihiro Nakamura, Aziz Awaad, Koichiro Hayashi, Kazuhiko Ochiai, Kazunori Ishimura, Thiol-Organosilica Particles Internally Functionalized with Propidium Iodide as a Multicolor Fluorescence and X-ray Computed Tomography Probe and Application for Non-Invasive Functional Gastrointestinal Tract Imaging, Chemistry of Materials, 24, 3772–3779, 2012.10. |
| 79. |
Koichiro Hayashi, Michihiro Nakamura, Hirokazu Miki, Shuji Ozaki, Masahiro Abe, Toshio Matsumoto, Kazunori Ishimura, Near-Infrared Fluorescent Silica/Porphyrin Hybrid Nanorings for In Vivo Cancer Imaging, Advanced Functional Materials, 22, 3539–3546, 2012.09. |
| 80. |
Koichiro Hayashi, Kazuki Maeda, Makoto Moriya, Wataru Sakamoto, Toshinobu Yogo, In Situ Synthesis of Cobalt Ferrite Nanoparticle/Polymer Hybrid from a Mixed Fe-Co Methacrylate for Magnetic Hyperthermia, Journal of Magnetism and Magnetic Materials, 324, 3158–3164, 2012.09. |
| 81. |
Koichiro Hayashi, Michihiro Nakamura, Kazunori Ishimura, Silica-Porphyrin Hybrid Nanotubes for In Vivo Cell Tracking by Near-Infrared Fluorescence Imaging, Chemical Communications, 48, 3830–3832, 2012.02. |
| 82. |
Koichiro Hayashi, Michihiro Nakamura, Yoji Makita, Rise Fujiwara, Toshinari Kori, Kazunori Ishimura, Synthesis and Photocatalytic Activity of Sea Urchin-Shaped Rutile TiO2 Nanocrystals, Materials Letters, 65, 3037–3040, 2011.10. |
| 83. |
Koichiro Hayashi, Michihiro Nakamura, Wataru Sakamoto, Toshinobu Yogo, Toshinari Kori, Kazunori Ishimura, Formation of TiO2 Nanostructures by Enzyme-Mediated Self-Assembly for the Destruction of Macrophages, Chemistry of Materials, 23, 3341–3347, 2011.07. |
| 84. |
Koichiro Hayashi, Michihiro Nakamura, Kazunori Ishimura, In Situ Synthesis and Photoresponsive Rupture of Organosilica Nanocapsules, Chemical Communications, 47, 1518–1520, 2010.11. |
| 85. |
Koichiro Hayashi, Kenji Ono, Hiromi Suzuki, Makoto Sawada, Makoto Moriya, Wataru Sakamoto, Toshinobu Yogo, Electrosprayed Synthesis of Red-Blood-Cell-Like Particles with Dual-Modality for Magnetic Resonance and Fluorescence Imaging, Small, 6, 2384–2391, 2010.11. |
| 86. |
Koichiro Hayashi, Kenji Ono, Hiromi Suzuki, Makoto Sawada, Makoto Moriya, Wataru Sakamoto, Toshinobu Yogo, High-Frequency, Magnetic Field-Responsive Drug Release from Magnetic Nanoparticle/Organic Hybrid Based on Hyperthermic Effect, ACS Applied Materials & Interfaces, 2, 1903–1911, 2010.07. |
| 87. |
Koichiro Hayashi, Kenji Ono, Hiromi Suzuki, Makoto Sawada, Makoto Moriya, Wataru Sakamoto, Toshinobu Yogo, One-Pot Biofunctionalization of Magnetic Nanoparticles via Thiol-Ene Click Reaction for Magnetic Hyperthermia and Magnetic Resonance Imaging, Chemistry of Materials, 22, 3768–3772, 2010.06. |
| 88. |
Koichiro Hayashi, Makoto Moriya, Wataru Sakamoto, Toshinobu Yogo, Chemoselective Synthesis of Folic Acid-Functionalized Magnetite Nanoparticles via Click Chemistry for Magnetic Hyperthermia, Chemistry of Materials, 21, 1318–1325, 2009.04. |
| 89. |
Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, Magnetic and Rheological Properties of Monodisperse Fe3O4 Nanoparticle/Organic Hybrid, Journal of Magnetism and Magnetic Materials, 321, 450–457, 2009.03. |
| 90. |
Koichiro Hayashi, Toshifumi Shimizu, Hidefumi Asano, Wataru Sakamoto, Toshinobu Yogo, Synthesis of Spinel Iron Oxide Nanoparticle/Organic Hybrid for Hyperthermia, Journal of Materials Research, 23, 3415–3424, 2008.12. |
| 91. |
Koichiro Hayashi, Rintaro Fujikawa, Wataru Sakamoto, Mitsuteru Inoue, Toshinobu Yogo, Synthesis of Highly Transparent Lithium Ferrite Nanoparticle/Polymer Hybrid Self-standing Films Exhibiting Faraday Rotation in the Visible Region, Journal of Physical Chemistry C, 112, 14255–14261, 2008.09. |
| 92. |
Koichiro Hayashi, Wataru Sakamoto, Toshinobu Yogo, In Situ Synthesis of Lithium Ferrite Nanoparticle/Polymer Hybrid, Journal of Materials Research, 22, 974–981, 2007.04. |
