Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips Reviewed International journal

Yusuke Sakai, Yukiko Yoshiura, Kohji Nakazawa

Journal of Bioscience and Bioengineering   111 ( 1 )   85 - 91   2011.1

Technique for the control of spheroid diameter using microfabricated chips Reviewed International journal

Yusuke Sakai, Kohji Nakazawa

Acta Biomaterialia   3 ( 6 )   1033 - 1040   2007.11

Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing Reviewed International journal

Junji Fukuda, Yusuke Sakai, Kohji Nakazawa

Biomaterials   27 ( 7 )   1061 - 1070   2006.3

A first-in-human clinical study of laparoscopic autologous myoblast sheet transplantation to prevent delayed perforation after duodenal endoscopic mucosal dissection Reviewed International journal

Kengo Kanetaka, Yasuhiro Maruya, Miki Higashi, Shun Yamaguchi, Ryo Matsumoto, Shinichiro Kobayashi, Keiichi Hashiguchi, Fumiya Oohashi, Masaki Matsumura, Takahiro Naka, Yusuke Sakai, Kazuhiko Nakao, Shigeru Miyagawa, Susumu Eguchi

Cell Research & Therapy   15 ( 1 )   117 (13 pages)   2024.4

Subcutaneous angiogenesis induced by transdermal delivery of gel-in-oil nanogel dispersion Reviewed International journal

Yi Zhang, Jannatul Fardous, Yuuta Inoue, Ryota Doi, Asami Obata, Yusuke Sakai, Shinichi Aishima, Hiroyuki Ijima

Biomaterials Advances   154   213628   2023.9

Decellularized mouse liver as a small-scale scaffold for the creation of a miniaturized human liver Reviewed International journal

Hiroyuki Ijima, Yukako Fukuda, Mario Kokichi Uehara, Muhammad Shafiq, Fanqi Wu, Jaeyong Cho, Yusuke Sakai, Tatsunori Miyata, Takanobu Yamao, Yosuke Nakao, Yosuke Kuroda, Takashi Motomura, Yo-Ichi Yamashita, Hideo Baba

Journal of Chemical Engineering of Japan   56 ( 1 )   2204899   2023.5

Highly feasible procedure for laparoscopic transplantation of cell sheets under pneumoperitoneum in porcine model Reviewed International journal

Shun Yamaguchi, Kengo Kanetaka, Yasuhiro Maruya, Miki Higashi, Shinichiro Kobayashi, Keiichi Hashiguchi, Fumiya Oohashi, Yusuke Sakai, Kazuhiko Nakao, Susumu Eguchi

Surgical Endoscopy   36   3911 - 3919   2022.6

Successful induction of human chemically induced liver progenitors with small molecules from damaged liver Reviewed International journal

Takayuki Miyoshi, Masaaki Hidaka, Daisuke Miyamoto, Yusuke Sakai, Shunsuke Murakami, Yu Huang, Takanobu Hara, Akihiko Soyama, Kengo Kanetaka, Takahiro Ochiya, Susumu Eguchi

Journal of Gastroenterology   57 ( 6 )   441 - 452   2022.6

Functional changes of cocultured hepatocyte sheets subjected to continuous liver regeneration stimulation in cDNA-uPA/SCID mouse: Differences in transplantation sites Reviewed International journal

Daisuke Miyamoto, Yusuke Sakai, Yu Huang, Chihiro Yamasaki, Chise Tateno, Hideko Hasegawa, Tomomi Murai, Takanobu Hara, Tomohiko Adachi, Akihiko Soyama, Masaaki Hidaka, Shinichiro Ito, Kengo Kanetaka, Susumu Eguchi

Regenerative Therapy   2021.12

Creation of a novel lipid-trehalose derivative showing positive interaction with the cell membrane Reviewed International journal

Kozue Yoshida, Fumiyasu Ono, Takehiro Chouno, Shota Nakada, Yasuhiro Ikegami, Nana Shirakigawa, Yusuke Sakai, Hiroyuki Ijima

Journal of Bioscience and Bioengineering   132 ( 1 )   71 - 80   2021.7

Chemical conversion of aged hepatocytes into bipotent liver progenitor cells Reviewed International journal

Yu Huang, Daisuke Miyamoto, Pei-Lin Li, Yusuke Sakai, Takanobu Hara, Tomohiko Adachi, Akihiko Soyama, Masaaki Hidaka, Kengo Kanetaka, Wei-Li Gu, Susumu Eguchi

Hepatology Research   2021.3

Subcutaneous transplantation of engineered islet/adipose-derived mesenchymal stem cell sheets in diabetic pigs with total pancreatectomy Reviewed International journal

Mampei Yamashita, Toshiyuki Adachi, Tomohiko Adachi, Shinichiro Ono, Naomi Matsumura, Kyoichiro Maekawa, Yusuke Sakai, Masaaki Hidaka, Kengo Kanetaka, Tamotsu Kuroki, Susumu Eguchi

Regenerative Therapy   2021.3

Bile duct reconstruction using scaffold-free tubular constructs created by Bio 3D printer Reviewed International journal

Takashi Hamada, Anna Nakamura, Akihiko Soyama, Yusuke Sakai, Takayuki Miyoshi, Shun Yamaguchi, Masaaki Hidaka, Takanobu Hara, Tota Kugiyama, Mitsuhisa Takatsuki, Akihide Kamiya, Koichi Nakayama, Susumu Eguchi

Regenerative Therapy   2021.3

Exploiting synergistic effect of externally loaded bFGF and endogenous growth factors for accelerated wound healing using heparin functionalized PCL/gelatin co-spun nanofibrous patches Reviewed International journal

Akshat Joshi, Zhe Xu, Yasuhiro Ikegami, Kozue Yoshida, Yusuke Sakai, Akshay Joshi, Tejinder Kaur, Yosuke Nakao, Yo-ichi Yamashita, Hideo Baba, Shinichi Aishima, Neetu Singh, Hiroyuki Ijima

Chemical Engineering Journal   404   126518   2021.1

The efficacy of autologous myoblast sheet transplantation to prevent perforation after duodenal endoscopic submucosal dissection in porcine model Reviewed International journal

Ryo Matsumoto, Kengo Kanetaka, Yasuhiro Maruya, Shun Yamaguchi, Shinichiro Kobayashi, Daisuke Miyamoto, Ken Ohnita, Yusuke Sakai, Keiichi Hashiguchi, Kazuhiko Nakao, Susumu Eguchi

Cell Transplantation   2021

Differentiation of chemically induced liver progenitor cells to cholangiocytes: investigation of the optimal conditions Reviewed International journal

Yu Huang, Yusuke Sakai, Takanobu Hara, Takeshi Katsuda, Takahiro Ochiya, Wei-Li Gu, Daisuke Miyamoto, Takashi Hamada, Kengo Kanetaka, Tomohiko Adachi, Susumu Eguchi

Journal of Bioscience and Bioengineering   2020.11

The relationship between lymphangiogenesis and liver regeneration after partial hepatectomy in cholestatic mice Reviewed International journal

Takashi Hamada, Masaaki Hidaka, Mitsuhisa Takatsuki, Yusuke Sakai, Haung Yu, Koji Natsuda, Shinichiro Ono, Tomohiko Adachi, Akihiko Soyama, Susumu Eguchi

Lymphatic Research and Biology   18 ( 4 )   322 - 328   2020.8

A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application Reviewed International journal

Yoshiyuki Kasai, Ryo Takagi, Shinichiro Kobayashi, Toshiyuki Owaki, Naoyuki Yamaguchi, Hiroko Fukuda, Yusuke Sakai, Yoshinori Sumita, Nobuo Kanai, Hajime Isomoto, Kengo Kanetaka, Takeshi Ohki, Izumi Asahina, Kazuhiro Nagai, Kazuhiko Nakao, Naoya Takeda, Teruo Okano, Susumu Eguchi, Masayuki Yamato

Regenerative Therapy   14   87 - 94   2020.6

Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets Reviewed International journal

Takanobu Hara, Akihiko Soyama, Toshiyuki Adachi, Shinichiro Kobayashi, Yusuke Sakai, Yasuhiro Maruya, Tota Kugiyama, Masaaki Hidaka, Satomi Okada, Takashi Hamada, Kyoichiro Maekawa, Shinichiro Ono, Tomohiko Adachi, Mitsuhisa Takatsuki, Susumu Eguchi

Regenerative Therapy   14   79 - 86   2020.6

In vitro and in vivo fabrication of stable human hepatocyte tissue in combination with normal fibroblasts derived from donors of various ages Reviewed International journal

Yusuke Sakai, Makiko Koike, Tomomi Murai, Masaaki Hidaka, Akihiko Soyama, Mitsuhisa Takatsuki, Susumu Eguchi

Journal of Bioscience and Bioengineering   128 ( 6 )   766 - 772   2019.12

Fabrication of functional cell sheets with human thyrocytes from non-tumorous thyroid tissue Reviewed International journal

Yu Huang, Kosho Yamanouchi, Yusuke Sakai, Sayaka Kuba, Chika Sakimura, Michi Morita, Kengo Kanetaka, Mitsuhisa Takatsuki, Susumu Eguchi

Tissue Engineering and Regenerative Medicine   16 ( 5 )   491 - 499   2019.10

Development of bifunctional three-dimensional cysts from chemically induced liver progenitors Reviewed International journal

Yu Huang, Yusuke Sakai, Takanobu Hara, Takeshi Katsuda, Takahiro Ochiya, Tomohiko Adachi, Masaaki Hidaka, Weili Gu, Susumu Eguchi

Stem Cells International   3975689   2019.9

Role of HGF for reprogramming human liver progenitor cells: Non-essential but stimulative supplement Reviewed International journal

Yu Huang, Takayuki Miyoshi, Yusuke Sakai, Takanobu Hara, Wei-li Gu, Susumu Eguchi

Journal of Hepatology   71 ( 2 )   438 - 439   2019.8

Controlled cell morphology and liver-specific function of engineered primary hepatocytes by fibroblast layer cell densities Reviewed International journal

Yusuke Sakai, Makiko Koike, Daisuke Kawahara, Hideko Hasegawa, Tomomi Murai, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Fumihiko Fujita, Tamotsu Kuroki, Susumu Eguchi

Journal of Bioscience and Bioengineering   126 ( 2 )   246 - 257   2018.8

An engineered cell sheet composed of human islets and human fibroblast, bone marrow–derived mesenchymal stem cells, or adipose–derived mesenchymal stem cells: An in vitro comparison study Reviewed International journal

10 ( 3 )   e1445948   2018.5

Spontaneous hepatocyte migration towards an endothelial cell tube network Reviewed International journal

Zhassulan Baimakhanov, Yusuke Sakai, Kosho Yamanouchi, Masaaki Hidaka, Akihiko Soyama, Mitsuhisa Takatsuki, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   12 ( 3 )   e1767 - e1771   2018.3

In vivo construction of liver tissue by implantation of a hepatic non-parenchymal/adipose-derived stem cell sheet Reviewed International journal

Mio Fujii, Kosho Yamanouchi, Yusuke Sakai, Zhassulan Baimakhanov, Izumi Yamaguchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   12 ( 1 )   e287 - e295   2018.1

Improvement of anal function by adipose-derived stem cell sheets Reviewed International journal

Yusuke Inoue, Fumihiko Fujita, Izumi Yamaguchi, Hiroko Kinoe, Daisuke Kawahara, Yusuke Sakai, Tamotsu Kuroki, Susumu Eguchi

Digestive Surgery   35 ( 1 )   64 - 69   2018.1

Oral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation Reviewed International journal

Naoyuki Yamaguchi, Hajime Isomoto, Shinichiro Kobayashi, Nobuo Kanai, Kengo Kanetaka, Yusuke Sakai, Yoshiyuki Kasai, Ryo Takagi, Takeshi Ohki, Hiroko Fukuda, Tsutomu Kanda, Kazuhiro Nagai, Izumi Asahina, Kazuhiko Nakao, Masayuki Yamato, Teruo Okano, Susumu Eguchi

Scientific Reports   7 ( 1 )   17460   2017.12

Toyocamycin attenuates free fatty acid-induced hepatic steatosis and apoptosis in cultured hepatocytes and ameliorates nonalcoholic fatty liver disease in mice Reviewed International journal

Ikuko Takahara, Yuko Akazawa, Maiko Tabuchi, Katsuya Matsuda, Hisamitsu Miyaaki, Youko Kido, Yasuko Kanda, Naota Taura, Ken Ohnita, Fuminao Takeshima, Yusuke Sakai, Susumu Eguchi, Masahiro Nakashima, Kazuhiko Nakao

PLoS One   12 ( 3 )   e0170591   2017.3

A modified method for purifying gallbladder epithelial cells using fluorescence-activated cell sorting Reviewed International journal

Hajime Imamura, Tomohiko Adachi, Amane Kitasato, Yusuke Sakai, Shinichiro Ono, Takanobu Hara, Koji Natsuda, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

In Vivo   31 ( 2 )   169 - 173   2017.3

Cell sheet technology for the regeneration of gastrointestinal tissue using a novel gastric perforation rat model Reviewed International journal

Shiro Tanaka, Kengo Kanetaka, Mio Fujii, Shinichiro Ito, Yusuke Sakai, Shinichiro Kobayashi, Kosho Yamanouchi, Fumihiko Fujita, Tamotsu Kuroki, Susumu Eguchi

Surgery Today   47 ( 1 )   114 - 121   2017.1

Generating tissue-engineered intestinal epithelium from cultured Lgr5 stem cells in vivo Reviewed International journal

Zhassulan Baimakhanov, Yasuhiro Torashima, Akihiko Soyama, Yusuke Inoue, Yusuke Sakai, Mitsuhisa Takatsuki, Fumihiko Fujita, Kengo Kanetaka, Tamotsu Kuroki, Susumu Eguchi

Regenerative Therapy   5   46 - 48   2016.12

Human fibroblast sheet promotes human pancreatic islet survival and function in vitro Reviewed International journal

Hajime Matsushima, Tamotsu Kuroki, Tomohiko Adachi, Amae Kitasato, Shinichiro Ono, Takayuki Tanaka, Masataka Hirabaru, Naoki Kuroshima, Takanori Hirayama, Yusuke Sakai, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tatsuya Kin, James Shapiro, Susumu Eguchi

Cell Transplantation   25 ( 8 )   1525 - 1537   2016.8

Equal distribution of MSCs after hepatic ischemia reperfusion injury Reviewed International journal

Askhat Isbambetov, Zhassulan Baimakhanov, Akihiko Soyama, Masaaki Hidaka, Yusuke Sakai, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

Journal of Surgical Research   203 ( 2 )   360 - 367   2016.6

Up-regulated extracellular matrix components and inflammatory chemokines may impair the regeneration of cholestatic liver Reviewed International journal

Shuai Zhang, Tao-Sheng Li, Akihiko Soyama, Takayuki Tanaka, Chen Yan, Yusuke Sakai, Masaaki Hidaka, Ayaka Kinoshita, Koji Natsuda, Mio Fujii, Tota Kugiyama, Zhassulan Baimakhanov, Tamotsu Kuroki, Weili Gu, Susumu Eguchi

Scientific Reports   6   26540   2016.5

Efficacy of multi-layered hepatocyte sheet transplantation for radiation-induced liver damage and partial hepatectomy in a rat model Reviewed International journal

Zhassulan Baimakhanov, Kosho Yamanouchi, Yusuke Sakai, Makiko Koike, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Fumihiko Fujita, Kengo Kanetaka, Tamotsu Kuroki, Susumu Eguchi

Cell Transplantation   25 ( 3 )   549 - 558   2016.3

A method for performing islet transplantation using tissue-engineered sheets of islets and mesenchymal stem cells Reviewed International journal

Masataka Hirabaru, Tamotsu Kuroki, Tomohiko Adachi, Amae Kitasato, Shinichiro Ono, Takayuki Tanaka, Hajime Matsushima, Yusuke Sakai, Akihiko Soyama, Masaaki Hidaka, Kosho Yamanouchi, Mitsuhisa Takatsuki, Teruo Okano, Susumu Eguchi

Tissue Engineering Part C: Methods   21 ( 12 )   1205 - 1215   2015.12

Rapid production of engineered human primary hepatocyte/fibroblast sheets Reviewed International journal

Yusuke Sakai, Makiko Koike, Akihiko Soyama, Masaaki Hidaka, Tamotsu Kuroki, Susumu Eguchi

Data in Brief   5   498 - 501   2015.12

Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model Reviewed International journal

Izumi Muraoka, Mitsuhisa Takatsuki, Yusuke Sakai, Tetsuo Tomonaga, Akihiko Soyama, Masaaki Hidaka, Yoshitaka Hishikawa, Takehiko Koji, Rie Utoh, Kazuo Ohashi, Teruo Okano, Takashi Kanematsu, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   9 ( 11 )   E108 - E115   2015.11

Efficacy of multi-layered hepatocyte sheet transplantation for radiation-induced liver damage and partial hepatectomy in a rat model Reviewed International journal

Zhassulan Baimakhanov, Kosho Yamanouchi, Yusuke Sakai, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Fumihiko Fujita, Kengo Kanetaka, Tamotsu Kuroki, Susumu Eguchi

Journal of the American College of Surgeons   221 ( 4, Supplement 1 )   S157   2015.10

How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets Reviewed International journal

Ryo Takagi, Shinichiro Kobayashi, Masayuki Yamato, Toshiyuki Owaki, Yoshiyuki Kasai, Takahiro Hosoi, Yusuke Sakai, Kengo Kanetaka, Tokutaro Minamizato, Asuka Minematsu, Makoto Kondo, Nobuo Kanai, Naoyuki Yamaguchi, Kazuhiro Nagai, Yasushi Miyazaki, Naoya Takeda, Fumio Fukai, Izumi Asahina, Taiga Miyazaki, Shigeru Kohno, Masakazu Yamamoto, Kazuhiko Nakao, Susumu Eguchi, Teruo Okano

Regenerative Therapy   1   1 - 4   2015.6

Micropatterned culture of HepG2 spheroids using microwell chip with honeycomb-patterned polymer film Reviewed International journal

Hidekazu Yamazaki, Shun Goto, Koju Ito, Souichi Kohashi, Yuki Goto, Yukiko Yoshiura, Yusuke Sakai, Hiroshi Yabu, Masatsugu Shimomura, Kohji Nakazawa

Journal of Bioscience and Bioengineering   118 ( 4 )   455 - 460   2014.10

Rapid fabrication of engineered liver tissue using novel fibroblast system Reviewed International journal

Yusuke Sakai, Makiko Koike, Hideko Hasegawa, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Teruo Okano, Susumu Eguchi

ALTEX Proceedings   3 ( 1 )   44   2014.8

Detachably assembled microfluidic device for perfusion culture and post-culture analysis of a spheroid array Reviewed International journal

Yusuke Sakai, Koji Hattori, Fumiki Yanagawa, Shinji Sugiura, Toshiyuki Kanamori, Kohji Nakazawa

Biotechnology Journal   9 ( 7 )   971 - 979   2014.7

Construction of liver tissue in vivo with preparative partial hepatic irradiation and growth stimulus: investigations of less invasive techniques and progenitor cells Reviewed International journal

Kensuke Miyazaki, Kosho Yamanouchi, Yusuke Sakai, Izumi Yamaguchi, Mitsuhisa Takatsuki, Tamotsu Kuroki, Chandan Guha, Susumu Eguchi

Journal of Surgical Research   185 ( 2 )   889 - 895   2013.12

Characterization of mouse embryoid bodies cultured on microwell chips with different well sizes Reviewed International journal

Kohji Nakazawa, Yukiko Yoshiura, Haruka Koga, Yusuke Sakai

Journal of Bioscience and Bioengineering   116 ( 5 )   628 - 633   2013.11

A novel animal model of long-term sustainable anal sphincter dysfunction Reviewed International journal

Izumi Yamaguchi, Fumihiko Fujita, Kosho Yamanouchi, Takehiro Mishima, Daisuke Kawahara, Yusuke Sakai, Shinichiro Ito, Kengo Kanetaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

Journal of Surgical Research   184 ( 2 )   813 - 818   2013.10

Toshiyuki Kanamori. Reversibly-assebmled perfusion culture chip with microwell array for controllable spheroid culture and post-culture analysis Reviewed International journal

Shinji Sugiura, Koji Hattori, Yusuke Sakai, Kohji Nakazawa

Proc. microTAS 2012 conference   470 - 472   2012.12

Development of a multilayered fibroblast/hepatocyte sheet for regenerative medicine Reviewed International journal

Yusuke Sakai, Mitsuhisa Takatsuki, Kosho Yamanouchi, Akihiko Soyama, Izumi Muraoka, Rie Utoh, Kazuo Ohashi, Teruo Okano, Tamotsu Kuroki, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   6 ( Supplement 1 )   123   2012.9

Reproducible animal model for the study of anal sphincter dysfunction for regenerative medicine Reviewed International journal

Izumi Yamaguchi, Fumihiko Fujita, Kosho Yamanouchi, Daisuke Kawahara, Yusuke Sakai, Sinichiro Ito, Kengo Kanetaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   6 ( Supplement 1 )   396   2012.9

Micropatterned co-culture of rat hepatocytes with 3T3 cells using microstencil technique Reviewed International journal

Kohji Nakazawa, Yukako Shinmura, Ami Higuchi, Yusuke Sakai

Journal of Tissue Engineering and Regenerative Medicine   6 ( Supplement 1 )   374   2012.9

Construction of liver tissue in vivo with preparative hepatic irradiation and growth stimuli: lower invasive techniques and progenitor cells Reviewed International journal

Kosho Yamanouchi, Izumi Yamaguchi, Yusuke Sakai, Akihiko Soyama, Fumihiko Fujita, Mitsuhisa Takatsuki, Kengo Kanetaka, Tamotsu Kuroki, Chandan Guha, Susumu Eguchi

Journal of Tissue Engineering and Regenerative Medicine   6 ( Supplement 1 )   124   2012.9

Novel microchip technique for the transfer of spheroids as floating cultures to micropatterned-adherent cultures Reviewed International journal

Yusuke Sakai, Kohji Nakazawa

Journal of Biochips & Tissue chips   S4   001   2011.12

Effects of culture conditions on a micropatterned co-culture of rat hepatocytes with 3T3 cells Reviewed International journal

Kohji Nakazawa, Yukako Shinmura, Ami Higuchi, Yusuke Sakai

Journal of Bioprocessing & Biotechniques   S3   002   2011.9

Superior oxygen and glucose supply in perfusion cell cultures compared to static cell cultures demonstrated by simulations using the finite element method Reviewed International journal

Shinji Sugiura, Yusuke Sakai, Kohji Nakazawa, Toshiyuki Kanamori

Biomicrofluidics   5 ( 2 )   022202   2011.6

Effect of cell spot sizes on micropatterned cultures of rat hepatocytes Reviewed International journal

Kohji Nakazawa, Yukako Shinmura, Yukiko Yoshiura, Yusuke Sakai

Biochemical Engineering Journal   53 ( 1 )   85 - 91   2010.12

Effect of microwell chip structure on cell microsphere production of various animal cells Reviewed International journal

Yusuke Sakai, Shirou Yoshida, Yukiko Yoshiura, Ryuhei Mori, Tomoko Tamura, Kanji Yahiro, Hideki Mori, Yonehiro Kanemura, Mami Yamasaki, Kohji Nakazawa

Journal of Bioscience and Bioengineering   110 ( 2 )   223 - 229   2010.8

Alkoxyresorufin O-dealkylase assay using a rat hepatocyte spheroid microarray Reviewed International journal

Yusuke Sakai, Tomoko Tanaka, Junji Fukuda, Kohji Nakazawa

Journal of Bioscience and Bioengineering   109 ( 4 )   395 - 399   2010.4

Comparative analysis of gene expression in rat liver tissue and monolayer- and spheroid-cultured hepatocytes Reviewed International journal

Yusuke Sakai, Seita Yamagami, Kohji Nakazawa

Cells Tissues Organs   191 ( 4 )   281 - 288   2010.3

Co-cultured hepatocyte-spheroid chip constructed using the microfabrication technique Reviewed International journal

Yusuke Sakai, Kohji Nakazawa

Animal Cell Technology: Basic & Applied Aspects   16   183 - 189   2008.11

Micropatterned hepatocyte culture using microstencil technique Reviewed International journal

Yukako Shinmura, Ami Higuchi, Yusuke Sakai, Kohji Nakazawa

Animal Cell Technology: Basic & Applied Aspects   16   391 - 396   2008.11

Micropatterned organoid culture of rat hepatocytes and HepG2 cells Reviewed International journal

Ryuhei Mori, Yusuke Sakai, Kohji Nakazawa

Journal of Bioscience and Bioengineering   106 ( 3 )   237 - 242   2008.9

Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip Reviewed International journal

Tomoko Tamura, Yusuke Sakai, Kohji Nakazawa

Journal of Materials Science: Materials in Medicine   19 ( 5 )   2071 - 2077   2008.5

Two-dimensional microarray of hepatocyte spheroids Reviewed International journal

Yusuke Sakai, Ryuhei Mori, Kohji Nakazawa

BIOMATERIALS in REGENERATIVE MEDICINE   151 - 154   2006.10

マルチマイクロキャビティ構造を有するスフェロイドアレイ化チップ

堺裕輔、森龍平、中澤浩二

シンポジウム「細胞チップの最前線」  2007.7 

Development of Hepatocyte Spheroid Microarray International conference

Yusuke Sakai, Kohji Nakazawa

The first Japan-Korea joint symposium on bio-microsensing technology  2008.5 

肝細胞スフェロイドアレイの開発とその性能評価

堺裕輔、中澤浩二

第15回肝細胞研究会  2008.6 

界面設計に基づく細胞パターニング技術

堺裕輔、中澤浩二

第54回高分子夏季大学  2008.7 

マイクロスフェア形成のための微小環境設計

堺裕輔、中澤浩二

第57回高分子討論会  2008.9 

Co-cultured hepatocyte spheroid chip using microfabrication technique International conference

Yusuke Sakai, Kohji Nakazawa

The 21st Annual and International Meeting of the JAACT  2008.11 

Three-dimensional co-culture system of hepatocytes and fibroblasts using microchip technique International conference

Yusuke Sakai, Kohji Nakazawa

The TERMIS North America 2008 Annual Conference & Exposition  2005.7 

血管誘導を伴う皮下性ヒト肝組織構築での遺伝子発現解析

堺裕輔、高槻光寿、江口晋

第119回日本外科学会定期学術集会  2019.4 

Cell-chip Technology for 3D Co-culture of Hepatocytes and Fibroblasts International conference

Yusuke Sakai, Yukiko Yoshiura, Kohji Nakazawa

The 10th International Cell Transplant Congress  2009.4 

Expression of Liver Specific Functions in Rat Hepatocyte Spheroid Array International conference

Yusuke Sakai, Seita Yamagami, Kohji Nakazawa

2nd TERMIS World Congress in conjunction with 2009 Seoul Stem Cell Symposium  2009.8 

ハニカムフィルムを利用したスフェロイドアレイの開発

堺裕輔、伊藤晃寿、白土健太郎、田中賢、下村政嗣、中澤浩二

第58回高分子討論会  2009.9 

肝細胞マイクロパターニング条件と機能発現の関係

堺裕輔、新村友佳子、吉浦由貴子、中澤浩二

第2回化学工学3支部合同北九州大会  2009.10 

Gene expression analysis in microarray system of rat hepatocyte spheroid International conference

Yusuke Sakai, Seita Yamagami, Kohji Nakazawa

The second Japan-Korea joint symposium on bio-microsensing technology  2009.11 

共培養型スフェロイド形成と非実質細胞混合比の効果

堺裕輔、中澤浩二

第17回肝細胞研究会  2010.6 

マイクロチップを利用したスフェロイド転写技術の確立

堺裕輔、袁一丹、中澤浩二

第59回高分子討論会  2010.9 

PDMSマイクロ流路チップを用いたHepG2細胞スフェロイドの灌流培養

堺裕輔、服部浩二、杉浦慎治、金森敏幸、中澤浩二

第22回化学とマイクロ・ナノシステム研究会  2010.6 

Mass Production of Spherical Multicellular Aggregate using Microfabricated Chips International conference

Yusuke Sakai, Kohji Nakazawa

The TERMIS North America 2010 Annual Conference  2010.12 

灌流マイクロデバイスを用いた肝細胞スフェロイド培養

堺裕輔、服部浩二、杉浦慎治、金森敏幸、中澤浩二

化学工学会 第76年会  2011.3 

培養表面設計に基づく肝細胞組織体培養

堺裕輔、黒木保、高槻光寿、金高賢悟、藤田文彦、山之内孝彰、曽山明彦、村岡いづみ、田中貴之、田中史朗、山口泉、江口晋

第12回長崎障害者支援再生医療研究会  2011.8 

スフェロイド培養のための灌流マイクロデバイス

堺裕輔、服部浩二、杉浦慎治、吉浦由貴子、金森敏幸、中澤浩二、江口晋

化学工学会 第77年会  2012.3 

ヒト肝細胞シートによる肝再生を目指して ～ヒト肝細胞シート作製技術の確立～

堺裕輔、高槻光寿、山之内孝彰、曽山明彦、村岡いづみ、大橋一夫、江口晋

第48回日本肝臓学会総会  2012.6 

共培養技術に基づく重層化肝細胞シートの開発

堺裕輔、高槻光寿、山之内孝彰、曽山明彦、村岡いづみ、田中貴之、山口泉、木下綾華、原貴信、鵜頭理恵、大橋一夫、岡野光夫、黒木保、江口晋

第11回日本再生医療学会総会  2012.6 

線維芽細胞/肝細胞重層化シートの開発およびin vivo肝特異機能発現

堺裕輔、高槻光寿、山之内孝彰、曽山明彦、鵜頭理恵、大橋一夫、岡野光夫、黒木保、江口晋

第19回肝細胞研究会  2012.6 

Development of a multilayered fibroblast/hepatocyte sheet for regenerative medicine International conference

Yusuke Sakai, Mitsuhisa Takatsuki, Kosho Yamanouchi, Akihiko Soyama, Izumi Muraoka, Rie Utoh, Kazuo Ohashi, Teruo Okano, Tamotsu Kuroki, Susumu Eguchi

3rd TERMIS World Congress in Vienna  2012.9 

再生医療のための肝細胞組織工学

堺裕輔、小池真章子、山之内孝彰、曽山明彦、高槻光寿、黒木保、江口晋

第27回長崎DDS・再生医療研究会  2012.11 

Reversibly-assembled microfluidic device consists of microwells and microchannels for stable perfusion culture of spheroid array International conference

Yusuke Sakai, Koji Hattori, Shinji Sugiura, Toshiyuki Kanamori, Kohji Nakazawa

2012 IEEE EMBS Micro and Nanotechnology in Medicine Conference  2012.12 

Reversibly-assembled microfluidic device consists of microwells and microchannels for stable perfusion culture of spheroid array

Yusuke Sakai, Koji Hattori, Shinji Sugiura, Toshiyuki Kanamori, Kohji Nakazawa

2012.12 

ヒト初代肝細胞/線維芽細胞複合シートによる異所性肝組織構築

堺裕輔、小池真章子、長谷川英子、山之内孝彰、曽山明彦、高槻光寿、黒木保、鵜頭理恵、大橋一夫、岡野光夫、江口晋

第12回日本再生医療学会総会  2013.3 

異所性肝組織構築のための再生医療デバイスの創製

堺裕輔、小池真章子、山之内孝彰、曽山明彦、高槻光寿、黒木保、鵜頭理恵、大橋一夫、岡野光夫、江口晋

第68回日本消化器外科学会総会  2013.7 

ヒト初代肝細胞/線維芽細胞複合シートが創造する第二の肝臓

堺裕輔、高槻光寿、江口晋

2013.10 

ヒト初代肝細胞複合シート移植における支持細胞の種類の効果

堺裕輔、小池真章子、長谷川英子、山之内孝彰、日高匡章、曽山明彦、高槻光寿、黒木保、岡野光夫、江口晋

第13回日本再生医療学会総会  2014.3 

Rapid fabrication of engineered liver tissue using novel fibroblast system International conference

Yusuke Sakai, Makiko Koike, Hideko Hasegawa, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Teruo Okano, Susumu Eguchi

9th World Congress on Alternatives and Animal Use in the Life Sciences  2014.5 

細胞シート工学を利用した血管誘導ヒト肝組織の迅速な自己構築 Invited

堺裕輔、小池真章子、長谷川英子、山之内孝彰、曽山明彦、日高匡章、高槻光寿、黒木保、江口晋

第115回日本外科学会定期学術集会  2015.4 

Reconstruction of vascularized subcutaneous liver tissue for minimally invasive cell therapy International conference

Yusuke Sakai, Makiko Koike, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi

ASGCT 18th Annual Meeting  2015.5 

皮下性ヒト肝組織構築による肝疾患治療

堺裕輔、小池真章子、長谷川英子、山之内孝彰、曽山明彦、日高匡章、黒木保、江口晋

第22回肝細胞研究会  2015.6 

皮下性ヒト肝組織構築に伴う血管網と肝特異機能発現の評価

堺裕輔、小池真章子、山之内孝彰、曽山明彦、日高匡章、黒木保、江口晋

第15回日本再生医療学会総会  2016.3 

肝特異的な特徴を有するヒト肝組織の皮下構築と機能発現解析

堺裕輔、小池真章子、長谷川英子、山之内孝彰、曽山明彦、日高匡章、高槻光寿、江口晋

第23回肝細胞研究会  2016.7 

術後急性肝不全予防のための迅速な肝細胞複合シート作製

堺裕輔、小池真章子、曽山明彦、日高匡章、高槻光寿、江口晋

第16回日本再生医療学会総会  2017.3 

第二の肝臓を皮下に創る Invited

堺裕輔

ものづくりライフイノベーション　最先端科学技術融合セミナー（第1回）  2017.3 

細胞シート工学を利用した血管網を有する皮下培養型ヒト肝組織作製

堺裕輔、高槻光寿、江口晋

第103回日本消化器病学会総会  2017.4 

Time-dependent liver-specific gene expressions of vascularized subcutaneous human liver tissue International conference

Yusuke Sakai, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Susumu Eguchi

5th TERMIS World Congress in Kyoto  2018.9 

第二の肝臓を皮下に創る Invited

堺裕輔

フェニックスバイオ　セミナー  2018.10 

血管誘導を伴う皮下性ヒト肝組織構築

堺裕輔、日髙匡章、高槻光寿、江口晋

第38回長崎障害者支援再生医療研究会  2019.1 

管腔構造を有するヒト肝細胞／内皮細胞索状組織作製技術の確立

堺裕輔、中澤浩二、日高匡章、高槻光寿、江口晋

第16回日本再生医療学会総会  2019.3 

血管誘導を伴う皮下性ヒト肝組織構築での遺伝子発現解析

堺裕輔、高槻光寿、江口晋

第119回日本外科学会定期学術集会  2019.4 

管腔構造を有するヒト肝細胞／内皮細胞索状組織作製技術の確立

堺裕輔、中澤浩二、日髙匡章、高槻光寿、井嶋博之、江口晋

第1回細胞シート工学イノベーションフォーラム  2019.7 

肝臓再構築に向けた研究と展望 Invited

堺裕輔、井嶋博之、落谷孝広、江口晋

日本再生医療学会 第1回秋季科学シンポジウム  2019.10 

管腔構造を有するヒト肝細胞／内皮細胞索状組織作製

堺裕輔、中澤浩二、江口晋、井嶋博之

第55回九大生体材料・力学研究会  2019.12 

ヒト肝細胞複合シート作製におけるヒト線維芽細胞ドナー年齢の影響

堺裕輔、江口晋、井嶋博之

第19回日本再生医療学会総会  2020.5 

臓器特異的ECMゲルを使用したチャイニーズハムスター腎臓由来幹細胞からの嚢胞様組織形成

堺裕輔、久保 善弘、河邉 佳典、上平 正道、井嶋 博之

第73回日本生物工学会大会  2021.10 

再生医工学研究とDXへの期待

堺裕輔

デジタル社会創造研究部門 第２回研究会  2022.12 

胆汁排泄機能を再構築した肝組織の創製

堺裕輔、森翔一郎、西凌太朗、江口晋、井嶋博之

化学工学会第88年会  2023.3 

細胞を用いた肝再生医療の最前線

岡田尚子、堺裕輔、江口晋

2017.12

組織工学による低侵襲な肝疾患治療

堺裕輔、江口晋

細胞   2015.7

肝細胞移植

堺裕輔、江口晋

再生医療用語ハンドブック   2015.3

肝胆膵外科医からみた再生医療

江口晋、堺裕輔

再生医療における臨床研究と製品開発   2013.9

ミクロ組織体を利用した細胞チップの開発

中澤浩二、堺裕輔、吉田詩朗、吉浦由貴子

若手研究者の大きな可能性を秘めた研究開発 若手ケミカルエンジニアリング連絡会編   2009.7

界面設計に基づく動物細胞のマイクロパターニング技術

中澤浩二、堺裕輔

日本化学会 コロイドおよび界面化学ニュースレター   2007.1

座長

化学工学会第88年会  2023.3

Screening of academic papers

Role(s)： Peer review

2023

化学工学会　ポスター賞審査員

第59回化学関連支部合同九州大会  2022.7

Screening of academic papers

Role(s)： Peer review

2022

次世代アニマルセルインダストリー研究部会　優秀学生発表賞　座長・審査員

第73回日本生物工学会大会  2021.10

化学工学会　ポスター賞審査員

第58回化学関連支部合同九州大会  2021.7

Screening of academic papers

Role(s)： Peer review

2021

運営委員 International contribution

2020.10

Screening of academic papers

Role(s)： Peer review

2020

化学工学会　ポスター賞審査員

第56回化学関連支部合同九州大会  2019.7

Screening of academic papers

Role(s)： Peer review

2019

Screening of academic papers

Role(s)： Peer review

2018

Screening of academic papers

Role(s)： Peer review

2016

Screening of academic papers

Role(s)： Peer review

2015

Screening of academic papers

Role(s)： Peer review

2013

Screening of academic papers

Role(s)： Peer review

2012

表在性非乳頭十二指腸腫瘍に対する内視鏡治療と腹腔鏡手術と再生医療を組み合わせた革新的な術式の開発

2020 - 2022

科学研究費助成事業  日本医療研究開発機構研究費（AMED）、再生医療実用化研究事業　多能性幹細胞（iPS/ES細胞）、体性幹細胞等を用いて、企業の協力を得ながらプロトコールを組む医師主導治験

肝細胞リプログラミング制御による効率的な肝構成細胞への分化

2020

九州大学QRプログラム わかばチャレンジ

肝細胞／類洞／胆管を複合した新規肝再構築技術の創出

Grant number：19H04445  2019 - 2021

日本学術振興会  科学研究費助成事業  基盤研究(B)

低分子化合物によるヒト肝前駆細胞を用いた肝硬変治療

2019 - 2021

科学研究費助成事業  日本医療研究開発機構研究費（AMED）、再生医療実現拠点ネットワークプログラム（技術開発個別課題＜ﾄﾗﾝｽﾚｰｼｮﾅﾙ･ﾘｻｰﾁ2（非臨床POC取得）＞）

マイクロニードルを基盤とした着脱可能なNeo-Liverの創製

2019 - 2021

工学研究新分野開拓助成

増殖-分化制御システムを取り入れたCHK細胞を用いたバイオ医薬品生産細胞の構築

2019 - 2020

科学研究費助成事業  日本医療研究開発機構研究費（AMED）、次世代治療・診断実現のための創薬基盤技術開発事業（バイオ医薬品の高度製造技術の開発　先端的バイオ製造技術開発）

機能性胆管ネットワークの高密度形成技術の確立

2019 - 2020

上原記念生命科学財団 研究奨励金（研究助成）

機能性胆管ネットワークの高密度形成技術の確立

2019

九州大学QRプログラム わかばチャレンジ

肝毛細胆管－肝内胆管構造・機能のin vitro再構築

Grant number：18K19932  2018 - 2020

日本学術振興会  科学研究費助成事業  挑戦的研究（萌芽）

肝細胞－内皮細胞索状組織移植による肝小葉構造を模倣した立体ヒト肝臓の創製

Grant number：16H05908  2016 - 2018

科学研究費助成事業  若手研究(A,B)

安全かつ有効な膵島細胞／間葉系幹細胞複合シートの皮下パッチ技術の開発

2016 - 2018

科学研究費助成事業  日本医療研究開発機構研究費（AMED）、難治性疾患等実用化研究事業 免疫アレルギー疾患等実用化研究事業

術後急性肝不全予防のための自己ヒト肝細胞移植技術の確立

2016 - 2018

武田科学振興財団 医学系研究奨励継続助成

二次元細胞配列／三次元培養による立体ヒト肝組織作製

2016 - 2017

上原記念生命科学財団 研究奨励金（研究助成）

ハイブリッド人工胆管構築技術を基盤とした術後胆管狭窄予防

Grant number：15K01295  2015 - 2017

日本学術振興会  科学研究費助成事業  基盤研究(C)

迅速な肝細胞複合シート作製技術を基盤とした急性肝不全治療

Grant number：26461916  2014 - 2016

日本学術振興会  科学研究費助成事業  基盤研究(C)

術後急性肝不全予防のための自己ヒト肝細胞移植技術の確立

2014 - 2016

武田科学振興財団 医学系研究奨励

脂肪組織由来多能性幹細胞移植による肝代謝性疾患治療の試み

Grant number：25462097  2013 - 2015

日本学術振興会  科学研究費助成事業  基盤研究(C)

ヒト肝細胞/非実質細胞複合シートによる異所性血管誘導肝組織の構築

Grant number：25861161  2013 - 2014

科学研究費助成事業  若手研究(A,B)

ヒト肝細胞/非実質細胞複合シートによる異所性血管誘導肝組織の構築

2013

長崎大学高度化推進経費 若手教員への研究支援事業

共培養技術に基づくヒト肝細胞シートの迅速作製法の開発

2012

長崎大学高度化推進経費 チャレンジ支援事業

微小培養環境設計による幹細胞の分化制御技術の確立

Grant number：09J08932  2009 - 2010

日本学術振興会  科学研究費助成事業  特別研究員奨励費

微小培養環境設計による幹細胞の分化制御技術の確立

2009 - 2010

日本学術振興会  特別研究員

再生医用工学

2023.12 - 2024.2   Winter quarter

生物化学工学

2023.12 - 2024.2   Winter quarter

課題協学

2023.10 - 2024.3   Second semester

化学工学実験第三

2023.10 - 2024.3   Second semester

化学工学実験第二

2023.4 - 2023.9   First semester

化学工学実験第一

2022.10 - 2023.3   Second semester

物質科学工学実験第三

2022.10 - 2023.3   Second semester

物質科学工学実験第二

2022.4 - 2022.9   First semester

物質科学工学実験第三

2021.10 - 2022.3   Second semester

物質科学工学実験第二

2021.4 - 2021.9   First semester

物質科学工学実験第三

2020.10 - 2021.3   Second semester

物質科学工学実験第一

2020.10 - 2021.3   Second semester

物質科学工学実験第二

2020.4 - 2020.9   First semester

物質科学工学実験第三

2019.10 - 2020.3   Second semester

物質科学工学実験第二

2019.4 - 2019.9   First semester

2019.9   Role：Participation   Title：産学連携　マネジメントFD

Organizer：[Undergraduate school/graduate school/graduate faculty]

2019.4   Role：Participation   Title：平成３１年度 第１回全学ＦＤ（新任教員の研修）

Organizer：University-wide