1. |
Ikumi Imajo, Tomohiro Yamada, Takahiro Fujinaga, Koki Nagano, Wataru Kumamaru, Toru Chikui, Tamotsu Kiyoshima, Mandibular metastasis from prostate cancer mimicked osteosarcoma, a case report., Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 2023.02. |
2. |
Minami Shibuya, Kotaro Ishii, Koki Nagano, Takahiro Fujinaga, Yuta Yanai, Tomohiro Yamada, Yuko Wada, Mayumi Shimizu, Yoshihide Mori , Paget’s disease of bone involving the mandible may causes temporomandibular joint ankylosis: A case report, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 2023.01. |
3. |
Koki Nagano, Tomohiro Yamada, Kotaro Ishii, Takahiro Fujinaga, Minami Shibuya, Yuta Yanai, Tamotsu Kiyoshima, Yoshihide Mori, A case of AIDS-associated Kaposi’s sarcoma occurring in the oral cavity and systemic lymph nodes, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, 2022.05. |
4. |
Takahiro Fujinaga, Yukiko Ohyama, Koki Nagano, Ikumi Imajo, Tomohiro Yamada, Tamotsu Kiyoshima, Yoshihide Mori, Methotrexate-associated lymphoproliferative disorder affecting the gingiva and intestinal mucosa with intestinal perforation: A case report, Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology,, 2022.03. |
5. |
Kana Ishibashi, Kotaro Ishii, Goro Sugiyama, Tomoki Sumida, Tsuyoshi Sugiura, Yu Kamata, Katsuhiro Seki, Takahiro Fujinaga, Wataru Kumamaru, Yosuke Kobayashi, Naomi Hiyake, Hiroyuki Nakano, Tomohiro Yamada, Yoshihide Mori, Deregulation of nicotinamide N-methyltransferase and gap junction protein alpha-1 causes metastasis in adenoid cystic carcinoma, Anticancer research, 10.21873/anticanres.12207, 38, 1, 187-197, 2018.01, Background/Aim: Adenoid cystic carcinoma (AdCC) is a malignant tumor that occurs in the salivary glands and frequently metastasizes. The aim of this study was to identify factors mediating AdCC metastasis. Materials and Methods: We established three AdCC cell lines by orthotropic transplantation and in vivo selection: parental, highly metastatic (ACCS-M-GFP), and lymph node metastatic (ACCS-LN-GFP) cells. Results: We examined the three cell lines. DNA microarray indicated significantly altered processes in ACCS-LN-GFP cells: particularly, the expression of nicotinamide N-methyltransferase (NNMT) was enhanced the most. NNMT is associated with tumorigenesis and is a potential tumor biomarker. Concomitantly, we found-significant down-regulation of gap junction protein alpha-1. We suggest that ACCS-LN-GFP cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. Conclusion: NNMT is a potential biomarker of AdCC.. |
6. |
Takahiro Fujinaga, Wataru Kumamaru, Tsuyoshi Sugiura, Yosuke Kobayashi, Yukiko Ohyama, Tatsuya Ikari, Mitsuho Onimaru, Naonari Akimoto, Rumi Jogo, Yoshihide Mori, Biological characterization and analysis of metastasis-related genes in cell lines derived from the primary lesion and lymph node metastasis of a squamous cell carcinoma arising in the mandibular gingiva, International journal of oncology, 10.3892/ijo.2014.2332, 44, 5, 1614-1624, 2014.05, Controlling metastatic lesions is an important part of improving cancer prognosis, in addition to controlling the primary lesion. There have been numerous histological studies on primary and metastatic lesions, but little basic research has been performed using cell lines from primary and metastatic lesions belonging to the same patient. In this study, we successfully established a cell line derived from lower gingival carcinoma (WK2) as well as a line derived from secondary cervical lymph node metastasis (WK3F) through primary cultures of tissue from a patient with oral squamous cell carcinoma. We then investigated the biological characteristics of the cancer cell lines from these primary and metastatic lesions and analyzed metastasis-related genes. Comparison of the biological characteristics in vitro showed that WK3F had higher cell proliferation ability and shorter cell doubling time than WK2. WK3F also had increased cell migratory ability and higher invasive and self-replication abilities. Heterotransplantation into nude mice resulted in high tumor formation rates in the tongue and high metastasis rates in the cervical lymph nodes. Changes in WK2 and WK3F gene expression were then comprehensively analyzed using micro-arrays. Genes with increased expression in WK3F compared to WK2 were extracted when the Z-score was ≥2.0 and the ratio was ≥5.0, while genes with reduced expression in WK3F compared to WK2 were extracted when the Z-score was ≤-2.0 and the ratio was ≤0.2; differences were found in 604 genes. From these, MAGEC1 (88.0-fold), MMP-7 (18.6-fold), SNAI1 (6.6-fold), MACC1 (6.2-fold), and HTRA1 (0.012-fold) were selected as metastasis-related candidate genes. The results suggest that these molecules could be important for clarifying the mechanisms that regulate metastasis and provide new therapeutic targets for inhibiting tumor invasion.. |
7. |
Yuta Yanai, Tsuyoshi Sugiura, Ikumi Imajyo, Naoya Yoshihama, Naonari Akimoto, Yosuke Kobayashi, Kohei Hayashi, Takahiro Fujinaga, Kanemitsu Shirasuna, Yasuharu Takenoshita, Yoshihide Mori, Retrospective study of selective submandibular neck dissection versus radical neck dissection for N0 or N1 necks in level i patients with oral squamous cell carcinoma, Journal of Oncology, 10.1155/2012/634183, 2012.06, Objective. To evaluate the efficacy of selective submandibular neck dissection (SMND) in patients with oral squamous cell carcinoma (OSCC) with or without nodal metastasis. Patients. From a total of 384 patients with untreated OSCC who underwent radical excision, we identified 229 with clinically N0 necks and 68 with clinically N1 necks in level I. Main Outcome Measures. The Kaplan-Meier 5-year regional control and 5-year disease specific survival (DSS) were compared for SMND, radical neck dissection (RND), and modified radical neck dissection (MRND). Results. In clinically node-negative necks, the regional control rates were 85.2% with SMND and 83.3% with MRND (P=0.89), and 5-year DSS rates were 86.5% and 87.0%, respectively, (P=0.94). In clinically N1 necks, the regional control rates were 81.3% with SMND and 83.0% with RND (P=0.72), and the DSS rates were 81.3% and 80.0%, respectively, (P=0.94). Type of neck dissection was not significantly associated with regional control or DSS on either univariate or multivariate analysis using Cox's proportional hazard model. Conclusions. SMND can be effectively applied in elective and therapeutic management to patients with OSCC that are clinically assessed as N0 or N1 to level I of the neck.. |